Mercurial > repos > proteore > proteore_expression_levels_by_tissue
comparison README.rst @ 8:2c0bab71a436 draft
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author | proteore |
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date | Tue, 18 Dec 2018 09:48:29 -0500 |
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children | 0a0f6c37f05e |
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1 Wrapper for Get expression data by tissue Tool | 1 Wrapper for Get expression profiles by tissue Tool |
2 ================================================= | 2 ================================================= |
3 | 3 |
4 **Authors** | 4 **Authors** |
5 | 5 |
6 T.P. Lien Nguyen, Florence Combes, Yves Vandenbrouck CEA, INSERM, CNRS, Grenoble-Alpes University, BIG Institute, FR | 6 T.P. Lien Nguyen, Florence Combes, Yves Vandenbrouck CEA, INSERM, CNRS, Grenoble-Alpes University, BIG Institute, FR |
11 | 11 |
12 Contact support@proteore.org for any questions or concerns about the Galaxy implementation of this tool. | 12 Contact support@proteore.org for any questions or concerns about the Galaxy implementation of this tool. |
13 | 13 |
14 ------------------------------------------------- | 14 ------------------------------------------------- |
15 | 15 |
16 This tool retrieve information from Human Protein Atlas (https://www.proteinatlas.org/) | 16 This tool retrieve information from Human Protein Atlas (https://www.proteinatlas.org/) regarding the expression profiles of human genes both on the mRNA and protein level. |
17 regarding the expression profiles of human genes both on the mRNA and protein level. | |
18 | 17 |
19 A list of ENSG (Ensembl gene) IDs must be entered (either via a copy/paste or by choosing a file), | 18 A list of ENSG (Ensembl gene) IDs must be entered (either via a copy/paste or by choosing a file), if it's not the case, please use the ID_Convert tool from ProteoRE. |
20 if it's not the case, please use the ID_Convert tool from ProteoRE. | |
21 | 19 |
22 The resources from Human Protein Atlas that can be queried are the following: | 20 The resources from Human Protein Atlas that can be queried are the following: |
23 | 21 |
24 * **Human normal tissue data**: expression profiles for proteins in human tissues based on immunohistochemisty using tissue micro arrays. | 22 * **Human normal tissue data**: expression profiles for proteins in human tissues based on immunohistochemisty using tissue micro arrays. |
25 | 23 |
30 * **Human tumor tissue data**: staining profiles for proteins in human tumor tissue based on immunohistochemisty using tissue micro arrays and log-rank P value for Kaplan-Meier analysis of correlation between mRNA expression level and patient survival. | 28 * **Human tumor tissue data**: staining profiles for proteins in human tumor tissue based on immunohistochemisty using tissue micro arrays and log-rank P value for Kaplan-Meier analysis of correlation between mRNA expression level and patient survival. |
31 | 29 |
32 The tab-separated file includes Ensembl gene identifier ("Gene"), gene name ("Gene name"), tumor name ("Cancer"), the number of patients annotated for different staining levels ("High", "Medium", "Low" & "Not detected") and log-rank p values for patient survival and mRNA correlation ("prognostic - favourable", "unprognostic - favourable", "prognostic - unfavourable", "unprognostic - unfavourable"). | 30 The tab-separated file includes Ensembl gene identifier ("Gene"), gene name ("Gene name"), tumor name ("Cancer"), the number of patients annotated for different staining levels ("High", "Medium", "Low" & "Not detected") and log-rank p values for patient survival and mRNA correlation ("prognostic - favourable", "unprognostic - favourable", "prognostic - unfavourable", "unprognostic - unfavourable"). |
33 | 31 |
34 The data is based on The Human Protein Atlas version 18 and Ensembl version 88.38. | 32 The data is based on The Human Protein Atlas version 18 and Ensembl version 88.38. |
35 | |
36 ----- | |
37 | |
38 **Reliability score** | |
39 | |
40 Reliability score is divided into Enhanced, Supported, Approved, or Uncertain with respect | |
41 to the definitions from HPA: | |
42 | |
43 Enhanced - One or several antibodies with non-overlapping epitopes targeting the same gene | |
44 have obtained enhanced validation based on orthogonal or independent antibody validation method. | |
45 | |
46 Supported - Consistency with RNA-seq and/or protein/gene characterization data, | |
47 in combination with similar staining pattern if independent antibodies are available. | |
48 | |
49 Approved - Consistency with RNA-seq data in combination with inconsistency with, or lack of, | |
50 protein/gene characterization data. Alternatively, consistency with protein/gene characterization data | |
51 in combination with inconsistency with RNA-seq data. If independent antibodies are available, | |
52 the staining pattern is partly similar or dissimilar. | |
53 | |
54 Uncertain - Inconsistency with, or lack of, RNA-seq and/or protein/gene characterization data, | |
55 in combination with dissimilar staining pattern if independent antibodies are available. |