Mercurial > repos > proteore > proteore_expression_rnaseq_abbased
diff add_expression_data.xml @ 14:133309fd6875 draft
"planemo upload commit c4edbc8a0ffd86395f088cb5b6592f77db658ac7"
author | proteore |
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date | Fri, 24 Jan 2020 04:22:48 -0500 |
parents | 0b279190f90d |
children | 208b87582b4c |
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--- a/add_expression_data.xml Fri Jun 28 05:01:37 2019 -0400 +++ b/add_expression_data.xml Fri Jan 24 04:22:48 2020 -0500 @@ -1,28 +1,41 @@ -<tool id="rna_abbased_data" name="Add expression data" version="2019.06.27"> +<tool id="rna_abbased_data" name="Add expression data" version="2020.01.24"> <description> (RNAseq or Immuno-assays)[Human Protein Atlas] </description> <requirements> - <requirement type="package" version="3.4.1">R</requirement> + <requirement type="package" version="3.4.4">R</requirement> </requirements> <stdio> <exit_code range="1:" /> </stdio> <command><![CDATA[ - #if $inputtype.filetype == "copy_paste": - - Rscript $__tool_directory__/add_expression_HPA.R --inputtype="copypaste" --input='$inputtype.genelist' --atlas="$__tool_directory__/proteinatlas.csv" --select='$options.hpaparams' --output='$output' + Rscript $__tool_directory__/add_expression_HPA.R + --input='$inputtype.genelist' + --select='$options.hpaparams' + --output='$output' + + #if $inputtype.filetype == "copy_paste": + --inputtype="copypaste" + #else + --inputtype="tabfile" + --header='$inputtype.header' + --column='$inputtype.column' + #end if - #else - - Rscript $__tool_directory__/add_expression_HPA.R --inputtype="tabfile" --input='$inputtype.genelist' --header='$inputtype.header' --atlas="$__tool_directory__/proteinatlas.csv" --column='$inputtype.column' --select='$options.hpaparams' --output='$output' - - #end if - + #if "protein_atlas" in str($ref_file).split("/") + --atlas="$ref_file" + #else + --atlasf="$__tool_directory__/$ref_file" + #end if ]]></command> <inputs> + <param name="ref_file" type="select" label="HPA source file version" > + <options from_data_table="proteore_protein_full_atlas"> + <filter type="sort_by" column="0"/> + </options> + </param> <conditional name="inputtype"> <param name="filetype" type="select" label="Enter your IDs (Ensembl gene IDs only, e.g. ENSG00000064787)" help="Copy/paste or from a file (e.g. table)"> <option value="file_all" selected="true">Input file containing your IDs</option> @@ -54,12 +67,12 @@ <option value="Gene description" selected="false">Gene description</option> <option value="Evidence">Evidence (at protein level, at transcript level or no evidence)</option> <option value="Antibody">Antibody reference</option> - <option value="RNA tissue category">RNA tissue category</option> - <option value="Reliability (IH)">IH detection level</option> + <option value="RNA tissue specificity">RNA tissue category</option> + <option value="Reliability (IH)">IH detection level</option> <option value="Reliability (IF)">IF detection level</option> <option value="Subcellular location">Subcellular location</option> - <option value="RNA TS TPM">RNA tissue specificity abundance in 'Transcript Per Million'</option> - <option value="TPM max in non-specific">RNA non-specific tissue abundance in 'Transcript Per Million'</option> + <option value="RNA tissue specific NX">RNA tissue specificity abundance in 'Transcript Per Million'</option> + <option value="TPM max in non-specific">RNA non-specific tissue abundance in 'Transcript Per Million' (only for 23/10/2018 release)</option> </param> </section> @@ -124,7 +137,7 @@ - RNA tissue specificity abundance in 'Transcript Per Million': For each gene is reported the tissue specificity abundance in 'Transcript Per Million' (TPM) as the sum of the TPM values of all its protein-coding transcripts. -- RNA non-specific tissue abundance in 'Transcript Per Million': please refer to http://www.proteinatlas.org/about/assays+annotation#rna. +- RNA non-specific tissue abundance in 'Transcript Per Million' (only available from 23/10/2018 release): please refer to http://www.proteinatlas.org/about/assays+annotation#rna. ----- @@ -134,9 +147,13 @@ ----- -**Data sources (release date)** +**HPA source file version (release date)** + +Release date 23/10/2018: data are based on the Human Protein Atlas version 18.1 and Ensembl version 88.38 -HPA source file (data are based on the Human Protein Atlas version 18.1 and Ensembl version 88.38): http://www.proteinatlas.org/download/proteinatlas.tab.gz +Release date 21/01/2020: a subset of the data in the Human Protein Atlas version 19.1 + +downloaded from: http://www.proteinatlas.org/download/proteinatlas.tab.gz -----