Mercurial > repos > public-health-bioinformatics > adjust_bracken_for_unclassified_reads
comparison test-data/input/README.md @ 0:3ab9d37e547e draft
"planemo upload for repository https://github.com/public-health-bioinformatics/galaxy_tools/blob/master/tools/adjust_bracken_for_unclassified_reads commit 0d1d1f356cdfd8ef6dbcdd1bfe76c4637587ff53"
author | public-health-bioinformatics |
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date | Thu, 10 Mar 2022 21:35:14 +0000 |
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3 ## Obtain original sequence data | |
4 | |
5 ``` | |
6 wget ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR176/049/SRR17619849/SRR17619849_1.fastq.gz | |
7 wget ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR176/049/SRR17619849/SRR17619849_2.fastq.gz | |
8 wget ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR179/045/SRR17907745/SRR17907745_1.fastq.gz | |
9 wget ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR179/045/SRR17907745/SRR17907745_2.fastq.gz | |
10 ``` | |
11 | |
12 ## Obtain kraken2/bracken database | |
13 | |
14 large file ~38GB compressed, ~50GB uncompressed | |
15 | |
16 ``` | |
17 wget https://genome-idx.s3.amazonaws.com/kraken/k2_standard_20210517.tar.gz | |
18 ``` | |
19 | |
20 ## Run kraken2 | |
21 | |
22 ``` | |
23 kraken2 --db k2_standard_20210517 --report --report SRR17619849_kraken2.txt --paired SRR17619849_1.fastq.gz SRR17619849_2.fastq.gz | |
24 kraken2 --db k2_standard_20210517 --report --report SRR17907745_kraken2.txt --paired SRR17907745_1.fastq.gz SRR17907745_2.fastq.gz | |
25 ``` | |
26 | |
27 ## Run bracken | |
28 | |
29 ``` | |
30 bracken -d k2_standard_20210517 -i SRR17619849_kraken2.txt -o SRR17619849_bracken_abundances.tsv -r 250 -l S | |
31 bracken -d k2_standard_20210517 -i SRR17907745_kraken2.txt -o SRR17907745_bracken_abundances.tsv -r 150 -l S | |
32 ``` |