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author | q2d2 |
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date | Mon, 29 Aug 2022 19:25:44 +0000 |
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children | 86e36a2b44b0 |
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<?xml version='1.0' encoding='utf-8'?> <!-- Copyright (c) 2022, QIIME 2 development team. Distributed under the terms of the Modified BSD License. (SPDX: BSD-3-Clause) --> <!-- This tool was automatically generated by: q2galaxy (version: 2022.8.1) for: qiime2 (version: 2022.8.1) --> <tool name="qiime2 deblur denoise-other" id="qiime2__deblur__denoise_other" version="2022.8.0+q2galaxy.2022.8.1.2" profile="22.05" license="BSD-3-Clause"> <description>Deblur sequences using a user-specified positive filter.</description> <requirements> <container type="docker">quay.io/qiime2/core:2022.8</container> </requirements> <version_command>q2galaxy version deblur</version_command> <command detect_errors="aggressive">q2galaxy run deblur denoise_other '$inputs'</command> <configfiles> <inputs name="inputs" data_style="paths"/> </configfiles> <inputs> <param name="demultiplexed_seqs" type="data" format="qza" label="demultiplexed_seqs: SampleData[SequencesWithQuality | PairedEndSequencesWithQuality | JoinedSequencesWithQuality]" help="[required] The demultiplexed sequences to be denoised."> <options options_filter_attribute="metadata.semantic_type"> <filter type="add_value" value="SampleData[PairedEndSequencesWithQuality]"/> <filter type="add_value" value="SampleData[JoinedSequencesWithQuality]"/> <filter type="add_value" value="SampleData[SequencesWithQuality]"/> </options> <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['SampleData[JoinedSequencesWithQuality]', 'SampleData[PairedEndSequencesWithQuality]', 'SampleData[SequencesWithQuality]']</validator> </param> <param name="reference_seqs" type="data" format="qza" label="reference_seqs: FeatureData[Sequence]" help="[required] Positive filtering database. Keep all sequences aligning to these sequences."> <options options_filter_attribute="metadata.semantic_type"> <filter type="add_value" value="FeatureData[Sequence]"/> </options> <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['FeatureData[Sequence]']</validator> </param> <param name="trim_length" type="integer" value="" label="trim_length: Int" help="[required] Sequence trim length, specify -1 to disable trimming."/> <section name="__q2galaxy__GUI__section__extra_opts__" title="Click here for additional options"> <param name="left_trim_len" type="integer" min="0" value="0" label="left_trim_len: Int % Range(0, None)" help="[default: 0] Sequence trimming from the 5' end. A value of 0 will disable this trim."/> <param name="sample_stats" type="boolean" truevalue="__q2galaxy__::literal::True" falsevalue="__q2galaxy__::literal::False" label="sample_stats: Bool" help="[default: No] If true, gather stats per sample."/> <param name="mean_error" type="float" value="0.005" label="mean_error: Float" help="[default: 0.005] The mean per nucleotide error, used for original sequence estimate."/> <param name="indel_prob" type="float" value="0.01" label="indel_prob: Float" help="[default: 0.01] Insertion/deletion (indel) probability (same for N indels)."/> <param name="indel_max" type="integer" value="3" label="indel_max: Int" help="[default: 3] Maximum number of insertion/deletions."/> <param name="min_reads" type="integer" value="10" label="min_reads: Int" help="[default: 10] Retain only features appearing at least min_reads times across all samples in the resulting feature table."/> <param name="min_size" type="integer" value="2" label="min_size: Int" help="[default: 2] In each sample, discard all features with an abundance less than min_size."/> <param name="jobs_to_start" type="integer" value="1" label="jobs_to_start: Int" help="[default: 1] Number of jobs to start (if to run in parallel)."/> <param name="hashed_feature_ids" type="boolean" truevalue="__q2galaxy__::literal::True" falsevalue="__q2galaxy__::literal::False" checked="true" label="hashed_feature_ids: Bool" help="[default: Yes] If true, hash the feature IDs."/> </section> </inputs> <outputs> <data name="table" format="qza" label="${tool.name} on ${on_string}: table.qza" from_work_dir="table.qza"/> <data name="representative_sequences" format="qza" label="${tool.name} on ${on_string}: representative_sequences.qza" from_work_dir="representative_sequences.qza"/> <data name="stats" format="qza" label="${tool.name} on ${on_string}: stats.qza" from_work_dir="stats.qza"/> </outputs> <tests/> <help> QIIME 2: deblur denoise-other ============================= Deblur sequences using a user-specified positive filter. Outputs: -------- :table.qza: The resulting denoised feature table. :representative_sequences.qza: The resulting feature sequences. :stats.qza: Per-sample stats if requested. | Description: ------------ Perform sequence quality control for Illumina data using the Deblur workflow, including positive alignment-based filtering. Only forward reads are supported at this time. This mode of execution is particularly useful when operating on non-16S data. For example, to apply Deblur to 18S data, you would want to specify a reference composed of 18S sequences in order to filter out sequences which do not appear to be 18S. The assessment is performed by local alignment using SortMeRNA with a permissive e-value threshold. | </help> <citations> <citation type="bibtex">@article{cite1, author = {Amir, Amnon and McDonald, Daniel and Navas-Molina, Jose A and Kopylova, Evguenia and Morton, James T and Xu, Zhenjiang Zech and Kightley, Eric P and Thompson, Luke R and Hyde, Embriette R and Gonzalez, Antonio and Knight, Rob}, journal = {MSystems}, number = {2}, pages = {e00191--16}, publisher = {Am Soc Microbiol}, title = {Deblur rapidly resolves single-nucleotide community sequence patterns}, volume = {2}, year = {2017} } </citation> <citation type="doi">10.1038/s41587-019-0209-9</citation> </citations> </tool>