Mercurial > repos > q2d2 > qiime2__feature_table__normalize
comparison qiime2__feature_table__normalize.xml @ 0:36f8db68eca4 draft default tip
planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__feature_table commit b1fccfb38b4873cd4699743033449014a2978e7d
author | q2d2 |
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date | Mon, 05 May 2025 18:54:07 +0000 |
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1 <?xml version='1.0' encoding='utf-8'?> | |
2 <!-- | |
3 Copyright (c) 2025, QIIME 2 development team. | |
4 | |
5 Distributed under the terms of the Modified BSD License. (SPDX: BSD-3-Clause) | |
6 --> | |
7 <!-- | |
8 This tool was automatically generated by: | |
9 q2galaxy (version: 2025.4.0) | |
10 for: | |
11 qiime2 (version: 2025.4.0) | |
12 --> | |
13 <tool name="qiime2 feature-table normalize" id="qiime2__feature_table__normalize" version="2025.4.0+q2galaxy.2025.4.0" profile="22.05" license="BSD-3-Clause"> | |
14 <description>Normalize FeatureTable</description> | |
15 <xrefs> | |
16 <xref type="bio.tools">qiime2</xref> | |
17 </xrefs> | |
18 <requirements> | |
19 <container type="docker">quay.io/qiime2/amplicon:2025.4</container> | |
20 </requirements> | |
21 <version_command>q2galaxy version feature_table</version_command> | |
22 <command detect_errors="exit_code">q2galaxy run feature_table normalize '$inputs'</command> | |
23 <configfiles> | |
24 <inputs name="inputs" data_style="staging_path_and_source_path"/> | |
25 </configfiles> | |
26 <inputs> | |
27 <param name="table" type="data" format="qza" label="table: FeatureTable[Frequency]" help="[required] Feature table with gene counts."> | |
28 <options options_filter_attribute="metadata.semantic_type"> | |
29 <filter type="add_value" value="FeatureTable[Frequency]"/> | |
30 </options> | |
31 <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['FeatureTable[Frequency]']</validator> | |
32 </param> | |
33 <param name="method" type="select" label="method: Str % Choices('tpm')¹ | Str % Choices('fpkm')² | Str % Choices('tmm')³ | Str % Choices('uq')⁴ | Str % Choices('cuf')⁵ | Str % Choices('ctf')⁶ | Str % Choices('cpm')⁷" help="[required] Specify the normalization method to be used. Use FPKM or TPM for within comparisons and TMM, UQ, CUF or CTF for between sample camparisons. Check https://www.genialis.com/wp-content/uploads/2023/12/2023-Normalizing-RNA-seq-data-in-Python-with-RNAnorm.pdf for more information on the methods."> | |
34 <option value="__q2galaxy__::literal::None" selected="true">Selection required</option> | |
35 <option value="tpm">tpm (Str)</option> | |
36 <option value="fpkm">fpkm (Str)</option> | |
37 <option value="tmm">tmm (Str)</option> | |
38 <option value="uq">uq (Str)</option> | |
39 <option value="cuf">cuf (Str)</option> | |
40 <option value="ctf">ctf (Str)</option> | |
41 <option value="cpm">cpm (Str)</option> | |
42 <validator type="expression" message="Please verify this parameter.">value != '__q2galaxy__::literal::None'</validator> | |
43 </param> | |
44 <section name="__q2galaxy__GUI__section__extra_opts__" title="Click here for additional options"> | |
45 <param name="gene_length" type="data" format="qza" optional="true" label="gene_length: FeatureData[SequenceCharacteristics % Properties('length')]" help="[optional] Gene lengths of all genes in the feature table."> | |
46 <options options_filter_attribute="metadata.semantic_type"> | |
47 <filter type="add_value" value="FeatureData[SequenceCharacteristics % Properties('length')]"/> | |
48 </options> | |
49 <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ["FeatureData[SequenceCharacteristics % Properties('length')]"]</validator> | |
50 </param> | |
51 <param name="m_trim" type="float" min="0" max="1" optional="true" label="m_trim: Float % Range(0, 1, inclusive_end=True)" help="[optional] Two sided cutoff for M-values. Can only be used for methods TMM and CTF. (default = 0.3)"/> | |
52 <param name="a_trim" type="float" min="0" max="1" optional="true" label="a_trim: Float % Range(0, 1, inclusive_end=True)" help="[optional] Two sided cutoff for A-values. Can only be used for methods TMM and CTF. (default = 0.05)"/> | |
53 </section> | |
54 </inputs> | |
55 <outputs> | |
56 <data name="normalized_table" format="qza" label="${tool.name} on ${on_string}: normalized_table.qza" from_work_dir="normalized_table.qza"/> | |
57 </outputs> | |
58 <tests/> | |
59 <help> | |
60 QIIME 2: feature-table normalize | |
61 ================================ | |
62 Normalize FeatureTable | |
63 | |
64 | |
65 Outputs: | |
66 -------- | |
67 :normalized_table.qza: Feature table normalized with specified method. | |
68 | |
69 | | |
70 | |
71 Description: | |
72 ------------ | |
73 Normalize FeatureTable by gene length, library size and composition with common methods for RNA-seq. | |
74 | |
75 | |
76 | | |
77 | |
78 </help> | |
79 <citations> | |
80 <citation type="bibtex">@misc{cite1, | |
81 author = {Zmrzlikar, Jure and Žganec, Matjaž and Ausec, Luka and Štajdohar, Miha}, | |
82 month = {July}, | |
83 title = {RNAnorm: RNA-seq data normalization in Python}, | |
84 url = {https://github.com/genialis/RNAnorm}, | |
85 version = {2.1.0}, | |
86 year = {2023} | |
87 } | |
88 </citation> | |
89 <citation type="doi">10.1038/s41587-019-0209-9</citation> | |
90 </citations> | |
91 </tool> |