# HG changeset patch # User q2d2 # Date 1714080002 0 # Node ID bb971260ea0796665df2f50435636f8be58ab3ec planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__rescript commit 389df0134cd0763dcf02aac6e623fc15f8861c1e diff -r 000000000000 -r bb971260ea07 qiime2__rescript__extract_seq_segments.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/qiime2__rescript__extract_seq_segments.xml Thu Apr 25 21:20:02 2024 +0000 @@ -0,0 +1,73 @@ + + + + + Use reference sequences to extract shorter matching sequence segments from longer sequences based on a user-defined 'perc-identity' value. + + quay.io/qiime2/amplicon:2024.2 + + q2galaxy version rescript + q2galaxy run rescript extract_seq_segments '$inputs' + + + + + + + + + hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['FeatureData[Sequence]'] + + + + + + hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['FeatureData[Sequence]'] + +
+ + + +
+
+ + + + + + +QIIME 2: rescript extract-seq-segments +====================================== +Use reference sequences to extract shorter matching sequence segments from longer sequences based on a user-defined 'perc-identity' value. + + +Outputs: +-------- +:extracted_sequence_segments.qza: Extracted sequence segments from 'input-sequences' that succesfully aligned to 'reference-segment-sequences'. +:unmatched_sequences.qza: Sequences in 'input-sequences' that did not have matching sequence segments within 'reference-segment-sequences'. + +| + +Description: +------------ +This action provides the ability to extract a region, or segment, of sequence without the need to specify primer pairs. This is very useful in cases when one or more of the primer sequences are not present within the target sequences, which prevents extraction of the (amplicon) region through primer-pair searching. Here, VSEARCH is used to extract these segments based on a reference pool of sequences that only span the region of interest. + + +| + + + + 10.7717/peerj.2584 + 10.1371/journal.pcbi.1009581 + 10.1038/s41587-019-0209-9 + +
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