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author | q2d2 |
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date | Wed, 30 Oct 2024 19:54:39 +0000 |
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<?xml version='1.0' encoding='utf-8'?> <!-- Copyright (c) 2024, QIIME 2 development team. Distributed under the terms of the Modified BSD License. (SPDX: BSD-3-Clause) --> <!-- This tool was automatically generated by: q2galaxy (version: 2024.10.0) for: qiime2 (version: 2024.10.1) --> <tool name="qiime2 rescript orient-seqs" id="qiime2__rescript__orient_seqs" version="2024.10.0+q2galaxy.2024.10.0" profile="22.05" license="BSD-3-Clause"> <description>Orient input sequences by comparison against reference.</description> <xrefs> <xref type="bio.tools">qiime2</xref> </xrefs> <requirements> <container type="docker">quay.io/qiime2/amplicon:2024.10</container> </requirements> <version_command>q2galaxy version rescript</version_command> <command detect_errors="exit_code">q2galaxy run rescript orient_seqs '$inputs'</command> <configfiles> <inputs name="inputs" data_style="staging_path_and_source_path"/> </configfiles> <inputs> <param name="sequences" type="data" format="qza" label="sequences: FeatureData[Sequence]" help="[required] Sequences to be oriented."> <options options_filter_attribute="metadata.semantic_type"> <filter type="add_value" value="FeatureData[Sequence]"/> </options> <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['FeatureData[Sequence]']</validator> </param> <section name="__q2galaxy__GUI__section__extra_opts__" title="Click here for additional options"> <param name="reference_sequences" type="data" format="qza" optional="true" label="reference_sequences: FeatureData[Sequence]" help="[optional] Reference sequences to orient against. If no reference is provided, all the sequences will be reverse complemented and all parameters will be ignored."> <options options_filter_attribute="metadata.semantic_type"> <filter type="add_value" value="FeatureData[Sequence]"/> </options> <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['FeatureData[Sequence]']</validator> </param> <param name="threads" type="integer" min="1" max="255" value="1" label="threads: Int % Range(1, 256)" help="[default: 1] Number of computation threads to use (1 to 256). The number of threads should be lesser or equal to the number of available CPU cores."/> <param name="dbmask" type="select" label="dbmask: Str % Choices('none', 'dust', 'soft')" display="radio"> <option value="__q2galaxy__::literal::None" selected="true">None (Use default behavior)</option> <option value="none">none</option> <option value="dust">dust</option> <option value="soft">soft</option> </param> <conditional name="__q2galaxy__GUI__conditional__relabel__" label="relabel: Str"> <param name="__q2galaxy__GUI__select__" type="select" label="relabel: Str" help="[optional] Relabel sequences using the prefix string and a ticker (1, 2, 3, etc.) to construct the new headers. Use --sizeout to conserve the abundance annotations."> <option value="__q2galaxy__::control::default" selected="true">None (Use default behavior)</option> <option value="__q2galaxy__::control::provide">Provide a value</option> </param> <when value="__q2galaxy__::control::default"> <param name="relabel" type="hidden" value="__q2galaxy__::literal::None"/> </when> <when value="__q2galaxy__::control::provide"> <param name="relabel" type="text"> <sanitizer> <valid initial="string.printable"/> </sanitizer> </param> </when> </conditional> <param name="relabel_keep" type="select" label="relabel_keep: Bool" help="[optional] When relabeling, keep the original identifier in the header after a space." display="radio"> <option value="__q2galaxy__::literal::None" selected="true">None (Use default behavior)</option> <option value="__q2galaxy__::literal::True">Yes</option> <option value="__q2galaxy__::literal::False">No</option> </param> <param name="relabel_md5" type="select" label="relabel_md5: Bool" help="[optional] When relabeling, use the MD5 digest of the sequence as the new identifier. Use --sizeout to conserve the abundance annotations." display="radio"> <option value="__q2galaxy__::literal::None" selected="true">None (Use default behavior)</option> <option value="__q2galaxy__::literal::True">Yes</option> <option value="__q2galaxy__::literal::False">No</option> </param> <param name="relabel_self" type="select" label="relabel_self: Bool" help="[optional] Relabel sequences using the sequence itself as a label." display="radio"> <option value="__q2galaxy__::literal::None" selected="true">None (Use default behavior)</option> <option value="__q2galaxy__::literal::True">Yes</option> <option value="__q2galaxy__::literal::False">No</option> </param> <param name="relabel_sha1" type="select" label="relabel_sha1: Bool" help="[optional] When relabeling, use the SHA1 digest of the sequence as the new identifier. The probability of a collision is smaller than the MD5 algorithm." display="radio"> <option value="__q2galaxy__::literal::None" selected="true">None (Use default behavior)</option> <option value="__q2galaxy__::literal::True">Yes</option> <option value="__q2galaxy__::literal::False">No</option> </param> <param name="sizein" type="select" label="sizein: Bool" help="[optional] In de novo mode, abundance annotations (pattern `[>;]size=integer[;]`) present in sequence headers are taken into account." display="radio"> <option value="__q2galaxy__::literal::None" selected="true">None (Use default behavior)</option> <option value="__q2galaxy__::literal::True">Yes</option> <option value="__q2galaxy__::literal::False">No</option> </param> <param name="sizeout" type="select" label="sizeout: Bool" help="[optional] Add abundance annotations to the output FASTA files." display="radio"> <option value="__q2galaxy__::literal::None" selected="true">None (Use default behavior)</option> <option value="__q2galaxy__::literal::True">Yes</option> <option value="__q2galaxy__::literal::False">No</option> </param> </section> </inputs> <outputs> <data name="oriented_seqs" format="qza" label="${tool.name} on ${on_string}: oriented_seqs.qza" from_work_dir="oriented_seqs.qza"/> <data name="unmatched_seqs" format="qza" label="${tool.name} on ${on_string}: unmatched_seqs.qza" from_work_dir="unmatched_seqs.qza"/> </outputs> <tests/> <help> QIIME 2: rescript orient-seqs ============================= Orient input sequences by comparison against reference. Outputs: -------- :oriented_seqs.qza: Query sequences in same orientation as top matching reference sequence. :unmatched_seqs.qza: Query sequences that fail to match at least one reference sequence in either + or - orientation. This will be empty if no refrence is provided. | Description: ------------ Orient input sequences by comparison against a set of reference sequences using VSEARCH. This action can also be used to quickly filter out sequences that (do not) match a set of reference sequences in either orientation. Alternatively, if no reference sequences are provided as input, all input sequences will be reverse-complemented. In this case, no alignment is performed, and all alignment parameters (`dbmask`, `relabel`, `relabel_keep`, `relabel_md5`, `relabel_self`, `relabel_sha1`, `sizein`, `sizeout` and `threads`) are ignored. | </help> <citations> <citation type="doi">10.7717/peerj.2584</citation> <citation type="doi">10.1371/journal.pcbi.1009581</citation> <citation type="doi">10.1038/s41587-019-0209-9</citation> </citations> </tool>