annotate qiime2__rescript__trim_alignment.xml @ 1:f14be3f75d11 draft

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date Mon, 03 Jun 2024 23:31:13 +0000
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1 <?xml version='1.0' encoding='utf-8'?>
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2 <!--
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3 Copyright (c) 2024, QIIME 2 development team.
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5 Distributed under the terms of the Modified BSD License. (SPDX: BSD-3-Clause)
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6 -->
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7 <!--
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8 This tool was automatically generated by:
1
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9 q2galaxy (version: 2024.5.0)
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10 for:
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11 qiime2 (version: 2024.5.0)
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12 -->
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13 <tool name="qiime2 rescript trim-alignment" id="qiime2__rescript__trim_alignment" version="2024.5.0+q2galaxy.2024.5.0" profile="22.05" license="BSD-3-Clause">
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14 <description>Trim alignment based on provided primers or specific positions.</description>
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15 <requirements>
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16 <container type="docker">quay.io/qiime2/amplicon:2024.5</container>
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17 </requirements>
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18 <version_command>q2galaxy version rescript</version_command>
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19 <command detect_errors="exit_code">q2galaxy run rescript trim_alignment '$inputs'</command>
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20 <configfiles>
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21 <inputs name="inputs" data_style="staging_path_and_source_path"/>
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22 </configfiles>
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23 <inputs>
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24 <param name="aligned_sequences" type="data" format="qza" label="aligned_sequences: FeatureData[AlignedSequence]" help="[required] Aligned DNA sequences.">
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25 <options options_filter_attribute="metadata.semantic_type">
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26 <filter type="add_value" value="FeatureData[AlignedSequence]"/>
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27 </options>
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28 <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['FeatureData[AlignedSequence]']</validator>
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29 </param>
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30 <section name="__q2galaxy__GUI__section__extra_opts__" title="Click here for additional options">
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31 <conditional name="__q2galaxy__GUI__conditional__primer_fwd__" label="primer_fwd: Str">
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32 <param name="__q2galaxy__GUI__select__" type="select" label="primer_fwd: Str" help="[optional] Forward primer used to find the start position for alignment trimming.">
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33 <option value="__q2galaxy__::control::default" selected="true">None (Use default behavior)</option>
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34 <option value="__q2galaxy__::control::provide">Provide a value</option>
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35 </param>
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36 <when value="__q2galaxy__::control::default">
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37 <param name="primer_fwd" type="hidden" value="__q2galaxy__::literal::None"/>
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38 </when>
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39 <when value="__q2galaxy__::control::provide">
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40 <param name="primer_fwd" type="text">
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41 <sanitizer>
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42 <valid initial="string.printable"/>
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43 </sanitizer>
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44 </param>
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45 </when>
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46 </conditional>
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47 <conditional name="__q2galaxy__GUI__conditional__primer_rev__" label="primer_rev: Str">
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48 <param name="__q2galaxy__GUI__select__" type="select" label="primer_rev: Str" help="[optional] Reverse primer used to find the end position for alignment trimming.">
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49 <option value="__q2galaxy__::control::default" selected="true">None (Use default behavior)</option>
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50 <option value="__q2galaxy__::control::provide">Provide a value</option>
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51 </param>
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52 <when value="__q2galaxy__::control::default">
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53 <param name="primer_rev" type="hidden" value="__q2galaxy__::literal::None"/>
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54 </when>
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55 <when value="__q2galaxy__::control::provide">
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56 <param name="primer_rev" type="text">
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57 <sanitizer>
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58 <valid initial="string.printable"/>
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59 </sanitizer>
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60 </param>
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61 </when>
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62 </conditional>
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63 <param name="position_start" type="integer" min="1" optional="true" label="position_start: Int % Range(1, None)" help="[optional] Position within the alignment where the trimming will begin. If not provided, alignment will notbe trimmed at the beginning. If forward primer isspecified this parameter will be ignored."/>
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64 <param name="position_end" type="integer" min="1" optional="true" label="position_end: Int % Range(1, None)" help="[optional] Position within the alignment where the trimming will end. If not provided, alignment will not be trimmed at the end. If reverse primer is specified this parameter will be ignored."/>
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65 </section>
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66 </inputs>
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67 <outputs>
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68 <data name="trimmed_sequences" format="qza" label="${tool.name} on ${on_string}: trimmed_sequences.qza" from_work_dir="trimmed_sequences.qza"/>
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69 </outputs>
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70 <tests/>
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71 <help>
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72 QIIME 2: rescript trim-alignment
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73 ================================
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74 Trim alignment based on provided primers or specific positions.
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75
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76
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77 Outputs:
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78 --------
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79 :trimmed_sequences.qza: Trimmed sequence alignment.
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80
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81 |
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82
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83 Description:
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84 ------------
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85 Trim an existing alignment based on provided primers or specific, pre-defined positions. Primers take precedence over the positions,i.e. if both are provided, positions will be ignored.When using primers in combination with a DNA alignment, a new alignment will be generated to locate primer positions. Subsequently, start (5'-most) and end (3'-most) position from fwd and rev primer located within the new alignment is identified and used for slicing the original alignment.
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86
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87
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88 |
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89
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90 </help>
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91 <citations>
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92 <citation type="doi">10.1371/journal.pcbi.1009581</citation>
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93 <citation type="doi">10.1038/s41587-019-0209-9</citation>
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94 </citations>
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95 </tool>