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author | q2d2 |
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date | Wed, 30 Oct 2024 19:55:36 +0000 |
parents | f14be3f75d11 |
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<?xml version='1.0' encoding='utf-8'?> <!-- Copyright (c) 2024, QIIME 2 development team. Distributed under the terms of the Modified BSD License. (SPDX: BSD-3-Clause) --> <!-- This tool was automatically generated by: q2galaxy (version: 2024.10.0) for: qiime2 (version: 2024.10.1) --> <tool name="qiime2 rescript trim-alignment" id="qiime2__rescript__trim_alignment" version="2024.10.0+q2galaxy.2024.10.0" profile="22.05" license="BSD-3-Clause"> <description>Trim alignment based on provided primers or specific positions.</description> <xrefs> <xref type="bio.tools">qiime2</xref> </xrefs> <requirements> <container type="docker">quay.io/qiime2/amplicon:2024.10</container> </requirements> <version_command>q2galaxy version rescript</version_command> <command detect_errors="exit_code">q2galaxy run rescript trim_alignment '$inputs'</command> <configfiles> <inputs name="inputs" data_style="staging_path_and_source_path"/> </configfiles> <inputs> <param name="aligned_sequences" type="data" format="qza" label="aligned_sequences: FeatureData[AlignedSequence]" help="[required] Aligned DNA sequences."> <options options_filter_attribute="metadata.semantic_type"> <filter type="add_value" value="FeatureData[AlignedSequence]"/> </options> <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['FeatureData[AlignedSequence]']</validator> </param> <section name="__q2galaxy__GUI__section__extra_opts__" title="Click here for additional options"> <conditional name="__q2galaxy__GUI__conditional__primer_fwd__" label="primer_fwd: Str"> <param name="__q2galaxy__GUI__select__" type="select" label="primer_fwd: Str" help="[optional] Forward primer used to find the start position for alignment trimming. Provide as 5'-3'."> <option value="__q2galaxy__::control::default" selected="true">None (Use default behavior)</option> <option value="__q2galaxy__::control::provide">Provide a value</option> </param> <when value="__q2galaxy__::control::default"> <param name="primer_fwd" type="hidden" value="__q2galaxy__::literal::None"/> </when> <when value="__q2galaxy__::control::provide"> <param name="primer_fwd" type="text"> <sanitizer> <valid initial="string.printable"/> </sanitizer> </param> </when> </conditional> <conditional name="__q2galaxy__GUI__conditional__primer_rev__" label="primer_rev: Str"> <param name="__q2galaxy__GUI__select__" type="select" label="primer_rev: Str" help="[optional] Reverse primer used to find the end position for alignment trimming. Provide as 5'-3'."> <option value="__q2galaxy__::control::default" selected="true">None (Use default behavior)</option> <option value="__q2galaxy__::control::provide">Provide a value</option> </param> <when value="__q2galaxy__::control::default"> <param name="primer_rev" type="hidden" value="__q2galaxy__::literal::None"/> </when> <when value="__q2galaxy__::control::provide"> <param name="primer_rev" type="text"> <sanitizer> <valid initial="string.printable"/> </sanitizer> </param> </when> </conditional> <param name="position_start" type="integer" min="1" optional="true" label="position_start: Int % Range(1, None)" help="[optional] Position within the alignment where the trimming will begin. If not provided, alignment will notbe trimmed at the beginning. If forward primer isspecified this parameter will be ignored."/> <param name="position_end" type="integer" min="1" optional="true" label="position_end: Int % Range(1, None)" help="[optional] Position within the alignment where the trimming will end. If not provided, alignment will not be trimmed at the end. If reverse primer is specified this parameter will be ignored."/> <param name="n_threads" type="integer" min="1" value="1" label="n_threads: Int % Range(1, None)" help="[default: 1] Number of threads to use for primer-based trimming, otherwise ignored. (Use `auto` to automatically use all available cores)"/> </section> </inputs> <outputs> <data name="trimmed_sequences" format="qza" label="${tool.name} on ${on_string}: trimmed_sequences.qza" from_work_dir="trimmed_sequences.qza"/> </outputs> <tests/> <help> QIIME 2: rescript trim-alignment ================================ Trim alignment based on provided primers or specific positions. Outputs: -------- :trimmed_sequences.qza: Trimmed sequence alignment. | Description: ------------ Trim an existing alignment based on provided primers or specific, pre-defined positions. Primers take precedence over the positions,i.e. if both are provided, positions will be ignored.When using primers in combination with a DNA alignment, a new alignment will be generated to locate primer positions. Subsequently, start (5'-most) and end (3'-most) position from fwd and rev primer located within the new alignment is identified and used for slicing the original alignment. | </help> <citations> <citation type="doi">10.1371/journal.pcbi.1009581</citation> <citation type="doi">10.1038/s41587-019-0209-9</citation> </citations> </tool>