Mercurial > repos > qfabrepo > metadegalaxy_pear_stats
diff pear_stats.xml @ 0:ec62f17fcfe6 draft default tip
"planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/pear_stats commit 0db3cb4e9a87400bb2f8402ffc23334e24ad4b4e"
author | qfabrepo |
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date | Mon, 14 Sep 2020 04:50:28 +0000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/pear_stats.xml Mon Sep 14 04:50:28 2020 +0000 @@ -0,0 +1,100 @@ +<tool id="pearStat" name="PEAR Statistics" version="1.0.0"> + <description>Generate paired-end reads overlap Statistic from PEAR log file</description> + <version_command> + python ${__tool_directory__}/pear_stats.py --version + </version_command> + <command detect_errors="aggressive"><![CDATA[ + #set files="" + #for $index,$file in enumerate($input): + #set $files += str($file)+"," + #end for + #set $files=$files[:-1] + + #set names=','.join( [ str( $name ) for $name in $input.keys() ]) + + python $__tool_directory__/pear_stats.py -i "$files" -s "$names" -o $output + ]]> </command> + <inputs> + <param name="input" type="data_collection" format="txt" collection_type="list" label="PEAR LOG FILE"/> + </inputs> + <outputs> + <!--<data format="tabular" name="output" label="${tool.name}.${input.display_name}"/>--> + <data format="tabular" name="output" label="${tool.name}.log"/> + </outputs> + <tests> + <test> + <param name="input"> + <collection type="list"> + <element name="F3D0" value="F3D0.log" /> + <element name="F3D1" value="F3D1.log" /> + </collection> + </param> + <output name="output" file="test_output.txt"/> + </test> + </tests> + + <help> +** what it does ** + +Creates summary log file by extracting "Assembled reads", "Discarded reads" and "Not assembled reads" from PEAR_ log. + +.. _PEAR: https://sco.h-its.org/exelixis/web/software/pear/doc.html + +----- + +======= +Example +======= + +PEAR v0.9.6 [January 15, 2015] + +Citation - PEAR: a fast and accurate Illumina Paired-End reAd mergeR +Zhang et al (2014) Bioinformatics 30(5): 614-620 | doi:10.1093/bioinformatics/btt593 + +==================================== ======================================= +Field Parameter and output +------------------------------------ --------------------------------------- +Forward reads file.................: /mnt/galaxy/files/014/dataset_14938.dat +Reverse reads file.................: /mnt/galaxy/files/014/dataset_14939.dat +PHRED..............................: 33 +Using empirical frequencies........: NO +Statistical method.................: OES +Maximum assembly length............: 999999 +Minimum assembly length............: 50 +p-value............................: 0.010000 +Quality score threshold (trimming).: 0 +Minimum read size after trimming...: 1 +Maximal ratio of uncalled bases....: 1.000000 +Minimum overlap....................: 10 +Scoring method.....................: Scaled score +Threads............................: 2 + +Allocating memory..................: 200,000,000 bytes +Assemblying reads: 0% +Assemblying reads: 100% + +Assembled reads ...................: 906 / 990 (91.515%) +Discarded reads ...................: 0 / 990 (0.000%) +Not assembled reads ...............: 84 / 990 (8.485%) +Assembled reads file...............: pear.assembled.fastq +Discarded reads file...............: pear.discarded.fastq +Unassembled forward reads file.....: pear.unassembled.forward.fastq +Unassembled reverse reads file.....: pear.unassembled.reverse.fastq +==================================== ======================================= + + +----- + +========= +Resources +========= + +**Wrapper Authors** + +QFAB Bioinformatics (support@qfab.org) + + </help> + <citations> + <citation type="doi">10.1093/bioinformatics/btt593</citation> + </citations> +</tool>