Mercurial > repos > qfabrepo > metadegalaxy_phyloseq_net
diff phyloseq_net.xml @ 0:af6d9ad14a0f draft
"planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_net commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
author | qfabrepo |
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date | Mon, 14 Sep 2020 08:13:43 +0000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/phyloseq_net.xml Mon Sep 14 08:13:43 2020 +0000 @@ -0,0 +1,125 @@ +<tool id="phyloseq_net" name="Phyloseq Network Plot" version="1.24.2" hidden="false"> + <description>Phyloseq Network Plot</description> + <requirements> + <requirement type="package" version="3.4.1">r-base</requirement> + <requirement type="package" version="1.24.2">bioconductor-phyloseq</requirement> + <requirement type="package" version="1.20.0">bioconductor-deseq2</requirement> + <requirement type="package" version="1.10.4">r-data.table</requirement> + <requirement type="package" version="1.20.0">r-getopt</requirement> + <requirement type="package" version="9.18">ghostscript</requirement> + </requirements> + <version_command><![CDATA[ + echo $(R --version | grep version | grep -v GNU)", phyloseq version" $(R --vanilla --slave -e "library(phyloseq); cat(sessionInfo()\$otherPkgs\$phyloseq\$Version)" 2> /dev/null | grep -v -i "WARNING: ") + ]]></version_command> + <command detect_errors="exit_code"><![CDATA[ + Rscript '${__tool_directory__}/phyloseq_net.r' + #if str($file_source.file_source_selector) == "set_biom": + --infile='$input' + --metafile='$metadata_input' + --biom="${file_source.file_source_selector}" + --xcolumn="${xaxis_column}" + --lcolumn="${legend_column}" + --outdir="$htmlfile.files_path" + --htmlfile='$htmlfile' + #else: + --infile='$input' + --metafile='$metadata_input' + --biom="${file_source.file_source_selector}" + --obsfile="${file_source.obstable}" + --norm="${file_source.norm_bool}" + --xcolumn="${xaxis_column}" + --lcolumn="${legend_column}" + --outdir="$htmlfile.files_path" + --htmlfile='$htmlfile' + #end if + ]]></command> + + <inputs> + + <conditional name="file_source"> + <param name="file_source_selector" type="select" label="Choose a file type"> + <option value="set_biom" selected="True"> BIOM File</option> + <option value="set_table"> Count File </option> + </param> + <when value="set_biom"> + <param format="biom1" name="input" type="data" label="Input File"/> + + </when> + <when value="set_table"> + <param format="tabular" name="input" type="data" label="Input File"/> + <param format="tabular" name="obstable" type="data" label="Observation file"/> + <param name="norm_bool" type="boolean" truevalue="true" falsevalue="false" checked="false" label="is the data normalised?"/> + </when> + </conditional> + <param format="tabular" name="metadata_input" type="data" label="Metadata file"/> + <param name="xaxis_column" type="data_column" data_ref="metadata_input" use_header_names="TRUE" label="Select a group for distance matrix"/> + <param name="legend_column" type="data_column" data_ref="metadata_input" use_header_names="TRUE" label="Column used as legend"/> + </inputs> + + <outputs> + <data format="html" name="htmlfile" label="${tool.name}.html"/> + </outputs> + + <tests> + <test> <!-- Test #1: Test BIOM format input --> + <!-- <conditional name="file_source"> --> + <param name="file_source_selector" value="set_biom"/> + <param name="input" value="GP.biom" ftype="biom1" /> + <param name="norm_bool" value="true"/> + <param name="metadata_input" value="metadata.txt" ftype="tabular"/> + <param name="xaxis_column" value="5" /> + <param name="legend_column" value="4" /> + <!-- </conditional>--> + <output name="htmlfile" ftype="html" file="biom_out.html" /> + </test> + <test> <!-- Test #2: Test TABULAR format inputs --> + <!-- <conditional name="file_source"> --> + <param name="file_source_selector" value="set_table" /> + <param name="input" value="count.txt" ftype="tabular" /> + <param name="obstable" value="observation.txt" ftype="tabular" /> + <param name="metadata_input" value="metadata.txt" ftype="tabular" /> + <param name="norm_bool" value="true" /> + <param name="xaxis_column" value="5" /> + <param name="legend_column" value="4" /> + <!-- </conditional> --> + <output name="htmlfile" ftype="html" file="test.html" /> + </test> + </tests> + + <help> +**What it does** +Creates a correlation network plot between samples using an R package called phyloseq_. + +.. _phyloseq: https://joey711.github.io/phyloseq/plot_network-examples.html + +----- + +**Input** + + +- **Choose a file type** - choose the input file type (e.g., BIOM or count table) +- **Input file** - choose the input file +- **Observation** - appear only if the "count table" selected in "choose a file type" +- **is the data normalised** - appear only if the "count table" selected in "choose a file type" +- **Select a group for distance matrix** - This group will be used to calculate the distance matrix +- **Column used as legend** - select a group from the metadata for legend + + +.. _BIOM: http://biom-format.org/ + +----- +========= +Resources +========= + +**Wrapper Authors** + +QFAB Bioinformatics (support@qfab.org) + + </help> + <citations> + <citation type="doi">10.18129/B9.bioc.phyloseq</citation> + <citation type="dio">doi:10.1186/2047-217X-1-7</citation> + </citations> + +</tool>