# HG changeset patch
# User qfabrepo
# Date 1600071223 0
# Node ID af6d9ad14a0fe88b4cfb84de98d9f993dd2d9e98
"planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_net commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
diff -r 000000000000 -r af6d9ad14a0f phyloseq_net.r
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/phyloseq_net.r Mon Sep 14 08:13:43 2020 +0000
@@ -0,0 +1,147 @@
+library('getopt')
+library('data.table')
+suppressPackageStartupMessages(library('phyloseq'))
+suppressPackageStartupMessages(library('DESeq2'))
+
+Sys.setenv("DISPLAY"=":1")
+
+options(warn= -1)
+option_specification = matrix(c(
+ 'infile','i',2,'character',
+ 'metafile','m',2,'character',
+ 'biom','b',2,'character',
+ 'obsfile','o',2,'character',
+ 'norm','n',2,'logical',
+ 'xcolumn','x',2,'numeric',
+ 'lcolumn','l',2,'numeric',
+ 'outdir','d',2,'character',
+ 'htmlfile','h',2,'character'
+),byrow=TRUE,ncol=4);
+
+
+options <- getopt(option_specification)
+options(bitmapType="cairo")
+
+matrix.format<-function(x) {
+ m<-as.matrix(x[,-1])
+ rownames(m)<-x[,1]
+ m
+}
+
+
+gm_mean = function(x, na.rm=TRUE){ exp(sum(log(x[x > 0]), na.rm=na.rm) / length(x))}
+
+
+tax_col_norm <- c("Kingdom","Phylum","Class","Order","Family","Genus","Species")
+tax_col_extra <- c("None","Kingdom","Phylum","Class","Order","Family","Genus","Species")
+
+tax_col_norm_otu <- c("OTUID","Kingdom","Phylum","Class","Order","Family","Genus","Species")
+tax_col_extra_otu <- c("OTUID","None","Kingdom","Phylum","Class","Order","Family","Genus","Species")
+
+if (!is.null(options$outdir)) {
+ # Create the directory
+ dir.create(options$outdir,FALSE)
+}
+
+is.biom<-options$biom
+
+
+pdffile <- gsub("[ ]+", "", paste(options$outdir,"/pdffile.pdf"))
+pngfile_net <- gsub("[ ]+", "", paste(options$outdir,"/net.png"))
+htmlfile <- gsub("[ ]+", "", paste(options$htmlfile))
+
+if(is.biom=="set_biom"){
+
+galaxy_biom <- import_biom(options$infile)
+galaxy_map <- import_qiime_sample_data(options$metafile)
+
+number.of.tax.rank<-length(colnames(tax_table(galaxy_biom)))
+
+ if(number.of.tax.rank == 7){
+ colnames(tax_table(galaxy_biom)) <- tax_col_norm
+ } else {
+ colnames(tax_table(galaxy_biom)) <- tax_col_extra
+ }
+
+physeq_galaxy <- merge_phyloseq(galaxy_biom,galaxy_map)
+
+} else {
+
+count.table<-read.table(options$infile,header=T,sep="\t",comment.char="",stringsAsFactors = F)
+meta.table<-read.table(options$metafile,header=T,sep="\t",comment.char="",stringsAsFactors = F)
+tax.table<-read.table(options$obsfile,header=T,sep="\t",comment.char="",stringsAsFactors = F)
+
+
+colnames(count.table)<-gsub("^X","",colnames(count.table))
+colnames(meta.table)<-gsub("^X.","",colnames(meta.table))
+colnames(tax.table)<-gsub("^X.","",colnames(tax.table))
+
+count.table.formatted<-matrix.format(count.table)
+OTU<-otu_table(count.table.formatted,taxa_are_rows = TRUE)
+
+tax.table.new<-as.data.frame(cbind(tax.table[,1],t(as.data.table(strsplit(tax.table[,2],";")))))
+
+
+if(length(colnames(tax.table.new)) != length(tax_col_extra_otu)){
+colnames(tax.table.new)<-tax_col_norm_otu
+}else{
+colnames(tax.table.new)<-tax_col_extra_otu
+}
+
+tax.table.formatted<-matrix.format(tax.table.new)
+
+TAX<-tax_table(tax.table.formatted)
+
+physeq_galaxy <- phyloseq(OTU, TAX)
+
+
+galaxy_map<-meta.table
+
+rownames(galaxy_map)<-meta.table[,1]
+
+sampledata<-sample_data(as.data.frame(galaxy_map,row.names=sample_names(galaxy_map),stringsAsFactos=F))
+
+sample_data(physeq_galaxy)<-sampledata
+
+}
+
+
+x.selectedColumn<-colnames(galaxy_map)[options$xcolumn]
+l.selectedColumn<-colnames(galaxy_map)[options$lcolumn]
+
+### normalisation
+if(is.null(options$norm) || options$norm =="false"){
+suppressMessages(raw.count.deseq2.obj<-phyloseq_to_deseq2(physeq_galaxy,as.formula(paste('~',x.selectedColumn,sep=""))))
+geoMeans = apply(counts(raw.count.deseq2.obj), 1, gm_mean)
+
+deseq.obj = estimateSizeFactors(raw.count.deseq2.obj, geoMeans = geoMeans)
+deseq.obj.norm<-otu_table(as.matrix(counts(deseq.obj,normalized=T)),taxa_are_rows=TRUE)
+
+otu_table(physeq_galaxy)<-deseq.obj.norm
+}
+
+
+# Produce PDF file
+
+pdf(pdffile);
+plot_net(physeq_galaxy,point_label=x.selectedColumn,color=l.selectedColumn)
+garbage<-dev.off();
+
+#Cairo(pngfile_net, type="png", bg="white",pointsize=12,dpi=100,units="in",width=6,height=6)
+png(pngfile_net,units="in",width=6,height=6,pointsize=12,res=100,bg="white")
+plot_net(physeq_galaxy,point_label=x.selectedColumn,color=l.selectedColumn)
+garbage<-dev.off()
+
+# Produce the HTML file
+htmlfile_handle <- file(htmlfile)
+html_output = c('
',
+ '',
+ '',
+ '',
+ '',
+ ' | ',
+ '
',
+ '
',
+ '');
+writeLines(html_output, htmlfile_handle);
+close(htmlfile_handle);
diff -r 000000000000 -r af6d9ad14a0f phyloseq_net.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/phyloseq_net.xml Mon Sep 14 08:13:43 2020 +0000
@@ -0,0 +1,125 @@
+
+ Phyloseq Network Plot
+
+ r-base
+ bioconductor-phyloseq
+ bioconductor-deseq2
+ r-data.table
+ r-getopt
+ ghostscript
+
+ /dev/null | grep -v -i "WARNING: ")
+ ]]>
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+**What it does**
+Creates a correlation network plot between samples using an R package called phyloseq_.
+
+.. _phyloseq: https://joey711.github.io/phyloseq/plot_network-examples.html
+
+-----
+
+**Input**
+
+
+- **Choose a file type** - choose the input file type (e.g., BIOM or count table)
+- **Input file** - choose the input file
+- **Observation** - appear only if the "count table" selected in "choose a file type"
+- **is the data normalised** - appear only if the "count table" selected in "choose a file type"
+- **Select a group for distance matrix** - This group will be used to calculate the distance matrix
+- **Column used as legend** - select a group from the metadata for legend
+
+
+.. _BIOM: http://biom-format.org/
+
+-----
+=========
+Resources
+=========
+
+**Wrapper Authors**
+
+QFAB Bioinformatics (support@qfab.org)
+
+
+
+ 10.18129/B9.bioc.phyloseq
+ doi:10.1186/2047-217X-1-7
+
+
+
diff -r 000000000000 -r af6d9ad14a0f test-data/GP.biom
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/GP.biom Mon Sep 14 08:13:43 2020 +0000
@@ -0,0 +1,1 @@
+{"id": "None","format": "Biological Observation Matrix 1.0.0","format_url": "http://biom-format.org","matrix_type": "sparse","generated_by": "BIOM-Format 2.1.5","date": "2020-04-06T10:47:50.459145","type": "OTU table","matrix_element_type": "float","shape": [3104, 20],"data": 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diff -r 000000000000 -r af6d9ad14a0f test-data/biom_out.html
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/biom_out.html Mon Sep 14 08:13:43 2020 +0000
@@ -0,0 +1,13 @@
+
+
+
+
+<<<<<<< HEAD
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+ |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000
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diff -r 000000000000 -r af6d9ad14a0f test-data/metadata.txt
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/metadata.txt Mon Sep 14 08:13:43 2020 +0000
@@ -0,0 +1,19 @@
+#SampleID dpw time Food Replicate_Group
+F3D0 0 Early None Group1
+F3D1 1 Early None Group1
+F3D2 2 Early None Group1
+F3D3 3 Early Cheddar Group2
+F3D5 5 Early Cheddar Group2
+F3D6 6 Early Cheddar Group2
+F3D7 7 Early Swiss Group3
+F3D8 8 Early Swiss Group3
+F3D9 9 Early Swiss Group3
+F3D141 141 Late Cheddar Group4
+F3D142 142 Late Cheddar Group4
+F3D143 143 Late Cheddar Group4
+F3D144 144 Late None Group5
+F3D145 145 Late None Group5
+F3D146 146 Late None Group5
+F3D147 147 Late Swiss Group6
+F3D148 148 Late Swiss Group6
+F3D149 149 Late Swiss Group6
diff -r 000000000000 -r af6d9ad14a0f test-data/observation.txt
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/observation.txt Mon Sep 14 08:13:43 2020 +0000
@@ -0,0 +1,3359 @@
+OTUID taxonomy
+775126 k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__; g__; s__
+228502 k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Ruminococcaceae; g__Ruminococcus; s__
+314713 k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Ruminococcaceae; g__Ruminococcus; s__
+314425 k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Ruminococcaceae; g__Ruminococcus; s__
+316122 k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__; g__; s__
+432015 k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__; g__; s__
+4374047 k__Bacteria; p__Actinobacteria; c__Coriobacteriia; o__Coriobacteriales; f__Coriobacteriaceae; g__Adlercreutzia; s__
+344726 k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Lachnospiraceae; g__; s__
+1078157 k__Bacteria; p__Firmicutes; c__Bacilli; o__Bacillales; f__Staphylococcaceae; g__Staphylococcus; s__
+974097 k__Bacteria; p__Firmicutes; c__Bacilli; o__Bacillales; f__Staphylococcaceae; g__Staphylococcus; s__
+323154 k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Lachnospiraceae; g__; s__
+266555 k__Bacteria; p__Actinobacteria; c__Coriobacteriia; o__Coriobacteriales; f__Coriobacteriaceae; g__Adlercreutzia; s__
+4316029 k__Bacteria; p__Firmicutes; c__Bacilli; o__Bacillales; f__Bacillaceae; g__; s__
+317867 k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__; g__; s__
+190425 k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__; g__; s__
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diff -r 000000000000 -r af6d9ad14a0f test-data/test.html
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/test.html Mon Sep 14 08:13:43 2020 +0000
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