Mercurial > repos > rmarenco > hubarchivecreator
annotate util/subtools.py @ 15:2a45cd656e8e draft
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit f11032a9df62c79a46f6e4a055a3c7ae981ea721
author | rmarenco |
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date | Fri, 02 Sep 2016 15:41:51 -0400 |
parents | 25809f699cb3 |
children | 3233451a3bd6 |
rev | line source |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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1 #!/usr/bin/python |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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2 # -*- coding: utf8 -*- |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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3 |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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4 """ |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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5 This class handles the subprocess calls of the different tools used |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
rmarenco
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6 in HubArchiveCreator |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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7 """ |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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8 |
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25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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9 import logging |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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10 import os |
fb5e60d4d18a
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11 import subprocess |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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12 import sys |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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13 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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14 class PopenError(Exception): |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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15 def __init__(self, cmd, error, return_code): |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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16 self.cmd = cmd |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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17 self.error = error |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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18 self.return_code = return_code |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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19 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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20 def __str__(self): |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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21 message = "The subprocess {0} has returned the error: {1}.".format(self.cmd, self.return_code) |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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22 message = ','.join((message, "Its error message is: {0}".format(self.error))) |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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23 return repr(message) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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24 |
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25 def _handleExceptionAndCheckCall(array_call, **kwargs): |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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26 """ |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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27 This class handle exceptions and call the tool. |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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28 It maps the signature of subprocess.check_call: |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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29 See https://docs.python.org/2/library/subprocess.html#subprocess.check_call |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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30 """ |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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31 stdin = kwargs.get('stdin') |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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32 stdout = kwargs.get('stdout') |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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33 stderr = kwargs.get('stderr') |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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34 shell = kwargs.get('shell') |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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35 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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36 cmd = array_call[0] |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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37 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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38 output = None |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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39 error = None |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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40 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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41 # TODO: Check the value of array_call and <=[0] |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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42 logging.debug("Calling {0}:".format(cmd)) |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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43 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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44 logging.debug("---------") |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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45 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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46 # TODO: Use universal_newlines option from Popen? |
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47 try: |
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48 p = subprocess.Popen(array_call, stdout=subprocess.PIPE, stderr=subprocess.PIPE, shell=shell) |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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49 output, error = p.communicate() |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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50 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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51 logging.debug("\t{0}".format(output)) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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52 # If we detect an error from the subprocess, then we raise an exception |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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53 # TODO: Manage if we raise an exception for everything, or use CRITICAL etc... but not stop process |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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54 # TODO: The responsability of returning a sys.exit() should not be there, but up in the app. |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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55 if p.returncode: |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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56 raise PopenError(cmd, error, p.returncode) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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57 |
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58 except OSError as e: |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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59 message = "The subprocess {0} has encountered an OSError: {1}".format(cmd, e.strerror) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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60 if e.filename: |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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61 message = '\n'.join((message, ", against this file: {0}".format(e.filename))) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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62 logging.error(message) |
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63 sys.exit(-1) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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64 except PopenError as p: |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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65 message = "The subprocess {0} has returned the error: {1}.".format(p.cmd, p.return_code) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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66 message = '\n'.join((message, "Its error message is: {0}".format(p.error))) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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67 |
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68 logging.exception(message) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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69 |
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70 sys.exit(p.return_code) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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71 except Exception as e: |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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72 message = "The subprocess {0} has encountered an unknown error: {1}".format(cmd, e) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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73 logging.exception(message) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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74 |
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75 sys.exit(-1) |
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76 return p |
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77 |
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78 |
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79 def twoBitInfo(two_bit_file_name, two_bit_info_file): |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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80 """ |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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81 Call twoBitInfo and write the result into twoBit_info_file |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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82 :param two_bit_file_name: |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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83 :param two_bit_info_file: |
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84 :return the subprocess.check_call return object: |
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85 """ |
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86 array_call = ['twoBitInfo', two_bit_file_name, two_bit_info_file] |
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87 p = _handleExceptionAndCheckCall(array_call) |
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88 return p |
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89 |
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90 |
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91 def faToTwoBit(fasta_file_name, twoBitFile): |
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92 """ |
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93 This function call faToTwoBit UCSC tool, and return the twoBitFile |
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94 :param fasta_file_name: |
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95 :param mySpecieFolder: |
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96 :return: |
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97 """ |
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98 |
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99 array_call = ['faToTwoBit', fasta_file_name, twoBitFile] |
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100 _handleExceptionAndCheckCall(array_call) |
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101 |
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102 return twoBitFile |
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103 |
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104 |
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105 def gtfToGenePred(input_gtf_file_name, gene_pred_file_name): |
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106 """ |
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107 Call gtfToGenePred and write the result into gene_pred_file_name |
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108 :param input_gtf_file_name: |
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109 :param gene_pred_file_name: |
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110 :return: |
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111 """ |
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112 array_call = ['gtfToGenePred', input_gtf_file_name, gene_pred_file_name] |
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113 p = _handleExceptionAndCheckCall(array_call) |
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114 return p |
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115 |
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116 |
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117 def gff3ToGenePred(input_gff3_file_name, gene_pred_file_name): |
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118 """ |
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119 Call gff3ToGenePred and write the result into gene_pred_file_name |
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120 :param input_gff3_file_name: |
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121 :param gene_pred_file_name: |
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122 :return: |
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123 """ |
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124 array_call = ['gff3ToGenePred', input_gff3_file_name, gene_pred_file_name] |
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125 p = _handleExceptionAndCheckCall(array_call) |
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126 return p |
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127 |
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128 def genePredToBigGenePred(gene_pred_file_name, unsorted_bigGenePred_file_name): |
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129 """ |
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130 Call genePredToBigGenePred and write the result into unsorted_bigGenePred_file_name |
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131 :param gene_pred_file_name: |
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132 :param unsorted_bigGenePred_file_name: |
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133 :return: |
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134 """ |
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135 array_call = ['genePredToBigGenePred', |
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136 gene_pred_file_name, |
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137 unsorted_bigGenePred_file_name] |
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138 p = _handleExceptionAndCheckCall(array_call) |
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139 return p |
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140 |
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141 def genePredToBed(gene_pred_file_name, unsorted_bed_file_name): |
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142 """ |
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143 Call genePredToBed and write the result into unsorted_bed_file_name |
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144 :param gene_pred_file_name: |
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145 :param unsorted_bed_file_name: |
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146 :return: |
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147 """ |
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148 array_call = ['genePredToBed', gene_pred_file_name, unsorted_bed_file_name] |
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149 p = _handleExceptionAndCheckCall(array_call) |
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150 return p |
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151 |
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152 |
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153 def sort(unsorted_bed_file_name, sorted_bed_file_name): |
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154 """ |
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155 Call sort with -k1,1 -k2,2n on unsorted_bed_file_name and write the result into sorted_bed_file_name |
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156 :param unsorted_bed_file_name: |
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157 :param sorted_bed_file_name: |
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158 :return: |
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159 """ |
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160 array_call = ['sort', '-k', '1,1', '-k', '2,2n', unsorted_bed_file_name, '-o', sorted_bed_file_name] |
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161 p = _handleExceptionAndCheckCall(array_call) |
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162 return p |
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163 |
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164 |
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165 def sortChromSizes(two_bit_info_file_name, chrom_sizes_file_name): |
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166 """ |
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167 Call sort with -k2rn on two_bit_info_file_name and write the result into chrom_sizes_file_name |
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168 :param two_bit_info_file_name: |
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169 :param chrom_sizes_file_name: |
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170 :return: |
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171 """ |
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172 array_call = ['sort', '-k2rn', two_bit_info_file_name, '-o', chrom_sizes_file_name] |
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173 p = _handleExceptionAndCheckCall(array_call) |
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174 return p |
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175 |
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176 |
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177 def bedToBigBed(sorted_bed_file_name, chrom_sizes_file_name, big_bed_file_name, |
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178 typeOption=None, autoSql=None, tab=False): |
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179 """ |
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180 Call bedToBigBed on sorted_bed_file_name, using chrom_sizes_file_name and write the result into big_bed_file_name |
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181 :param sorted_bed_file_name: |
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182 :param chrom_sizes_file_name: |
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183 :param big_bed_file_name: |
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184 :return: |
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185 """ |
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186 |
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187 # TODO: Move this into the _handleExceptionAndCheckCall function |
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188 # Parse the array |
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189 logging.debug("sorted_bed_file_name: {0}".format(sorted_bed_file_name)) |
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190 logging.debug("chrom_sizes_file_name: {0}".format(chrom_sizes_file_name)) |
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191 logging.debug("big_bed_file_name: {0}".format(big_bed_file_name)) |
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192 logging.debug("typeOption: {0}".format(typeOption)) |
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193 logging.debug("autoSql: {0}".format(autoSql)) |
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194 logging.debug("tab option: {0}".format(tab)) |
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195 |
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196 array_call = ['bedToBigBed', sorted_bed_file_name, chrom_sizes_file_name, big_bed_file_name] |
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197 if typeOption: |
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198 typeOption = ''.join(['-type=', typeOption]) |
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199 array_call.append(typeOption) |
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200 if autoSql: |
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201 autoSql = ''.join(['-as=', autoSql]) |
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202 array_call.append(autoSql) |
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203 if tab: |
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204 array_call.append('-tab') |
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205 |
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206 p = _handleExceptionAndCheckCall(array_call) |
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207 return p |
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208 |
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209 |
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210 def sortBam(input_bam_file_name, output_sorted_bam_name): |
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211 """ |
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212 Call samtools on input_bam_file_name and output the result in output_sorted_bam_name |
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213 :param input_bam_file_name: |
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214 :param output_sorted_bam_name: |
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215 :return: |
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216 """ |
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217 array_call = ['samtools', 'sort', input_bam_file_name, '-o', output_sorted_bam_name] |
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218 p = _handleExceptionAndCheckCall(array_call) |
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219 return p |
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220 |
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221 |
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222 def createBamIndex(input_sorted_bam_file_name, output_name_index_name): |
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223 """ |
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224 Call `samtools index` on imput_sorted_bam_file_name and output the result in output_name_index_name |
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225 :param input_sorted_bam_file_name: |
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226 :param output_name_index_name: |
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227 :return: |
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228 """ |
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229 array_call = ['samtools', 'index', input_sorted_bam_file_name, output_name_index_name] |
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230 p = _handleExceptionAndCheckCall(array_call) |
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231 return p |