Mercurial > repos > rmarenco > hubarchivecreator
comparison util/subtools.py @ 29:7e8a8b732db3 draft
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 1a81ebd0ddea950b84af3fc830e9267a4814b29f
author | yating-l |
---|---|
date | Wed, 16 May 2018 18:04:20 -0400 |
parents | df42241d3731 |
children |
comparison
equal
deleted
inserted
replaced
28:6aa28a85cc38 | 29:7e8a8b732db3 |
---|---|
11 import subprocess | 11 import subprocess |
12 import sys | 12 import sys |
13 import string | 13 import string |
14 import tempfile | 14 import tempfile |
15 | 15 |
16 | |
16 class PopenError(Exception): | 17 class PopenError(Exception): |
17 def __init__(self, cmd, error, return_code): | 18 def __init__(self, cmd, error, return_code): |
18 self.cmd = cmd | 19 self.cmd = cmd |
19 self.error = error | 20 self.error = error |
20 self.return_code = return_code | 21 self.return_code = return_code |
21 | 22 |
22 def __str__(self): | 23 def __str__(self): |
23 message = "The subprocess {0} has returned the error: {1}.".format(self.cmd, self.return_code) | 24 message = "The subprocess {0} has returned the error: {1}.".format( |
24 message = ','.join((message, "Its error message is: {0}".format(self.error))) | 25 self.cmd, self.return_code) |
26 message = ','.join( | |
27 (message, "Its error message is: {0}".format(self.error))) | |
25 return repr(message) | 28 return repr(message) |
29 | |
26 | 30 |
27 def _handleExceptionAndCheckCall(array_call, **kwargs): | 31 def _handleExceptionAndCheckCall(array_call, **kwargs): |
28 """ | 32 """ |
29 This class handle exceptions and call the tool. | 33 This class handle exceptions and call the tool. |
30 It maps the signature of subprocess.check_call: | 34 It maps the signature of subprocess.check_call: |
39 output = None | 43 output = None |
40 error = None | 44 error = None |
41 | 45 |
42 # TODO: Check the value of array_call and <=[0] | 46 # TODO: Check the value of array_call and <=[0] |
43 logging.debug("Calling {0}:".format(cmd)) | 47 logging.debug("Calling {0}:".format(cmd)) |
44 | 48 logging.debug("%s", array_call) |
45 logging.debug("---------") | 49 logging.debug("---------") |
46 | 50 |
47 # TODO: Use universal_newlines option from Popen? | 51 # TODO: Use universal_newlines option from Popen? |
48 try: | 52 try: |
49 p = subprocess.Popen(array_call, stdout=stdout, stderr=stderr, shell=shell) | 53 p = subprocess.Popen(array_call, stdout=stdout, |
54 stderr=stderr, shell=shell) | |
50 | 55 |
51 # TODO: Change this because of possible memory issues => https://docs.python.org/2/library/subprocess.html#subprocess.Popen.communicate | 56 # TODO: Change this because of possible memory issues => https://docs.python.org/2/library/subprocess.html#subprocess.Popen.communicate |
52 | 57 |
53 output, error = p.communicate() | 58 output, error = p.communicate() |
54 | 59 |
62 if p.returncode: | 67 if p.returncode: |
63 if stderr == subprocess.PIPE: | 68 if stderr == subprocess.PIPE: |
64 raise PopenError(cmd, error, p.returncode) | 69 raise PopenError(cmd, error, p.returncode) |
65 else: | 70 else: |
66 # TODO: To Handle properly with a design behind, if we received a option as a file for the error | 71 # TODO: To Handle properly with a design behind, if we received a option as a file for the error |
67 raise Exception("Error when calling {0}. Error as been logged in your file {1}. Error code: {2}"\ | 72 raise Exception("Error when calling {0}. Error as been logged in your file {1}. Error code: {2}" |
68 .format(cmd, stderr.name, p.returncode)) | 73 .format(cmd, stderr.name, p.returncode)) |
69 | 74 |
70 except OSError as e: | 75 except OSError as e: |
71 message = "The subprocess {0} has encountered an OSError: {1}".format(cmd, e.strerror) | 76 message = "The subprocess {0} has encountered an OSError: {1}".format( |
77 cmd, e.strerror) | |
72 if e.filename: | 78 if e.filename: |
73 message = '\n'.join((message, ", against this file: {0}".format(e.filename))) | 79 message = '\n'.join( |
80 (message, ", against this file: {0}".format(e.filename))) | |
74 logging.error(message) | 81 logging.error(message) |
75 sys.exit(-1) | 82 sys.exit(-1) |
76 except PopenError as p: | 83 except PopenError as p: |
77 message = "The subprocess {0} has returned the error: {1}.".format(p.cmd, p.return_code) | 84 message = "The subprocess {0} has returned the error: {1}.".format( |
78 message = '\n'.join((message, "Its error message is: {0}".format(p.error))) | 85 p.cmd, p.return_code) |
86 message = '\n'.join( | |
87 (message, "Its error message is: {0}".format(p.error))) | |
79 | 88 |
80 logging.exception(message) | 89 logging.exception(message) |
81 | 90 |
82 sys.exit(p.return_code) | 91 sys.exit(p.return_code) |
83 except Exception as e: | 92 except Exception as e: |
84 message = "The subprocess {0} has encountered an unknown error: {1}".format(cmd, e) | 93 message = "The subprocess {0} has encountered an unknown error: {1}".format( |
94 cmd, e) | |
85 logging.exception(message) | 95 logging.exception(message) |
86 | 96 |
87 sys.exit(-1) | 97 sys.exit(-1) |
88 return p | 98 return p |
99 | |
89 | 100 |
90 def twoBitInfo(two_bit_file_name, two_bit_info_file): | 101 def twoBitInfo(two_bit_file_name, two_bit_info_file): |
91 """ | 102 """ |
92 Call twoBitInfo and write the result into twoBit_info_file | 103 Call twoBitInfo and write the result into twoBit_info_file |
93 :param two_bit_file_name: | 104 :param two_bit_file_name: |
96 """ | 107 """ |
97 array_call = ['twoBitInfo', two_bit_file_name, two_bit_info_file] | 108 array_call = ['twoBitInfo', two_bit_file_name, two_bit_info_file] |
98 p = _handleExceptionAndCheckCall(array_call) | 109 p = _handleExceptionAndCheckCall(array_call) |
99 return p | 110 return p |
100 | 111 |
112 | |
101 def faToTwoBit(fasta_file_name, twoBitFile): | 113 def faToTwoBit(fasta_file_name, twoBitFile): |
102 """ | 114 """ |
103 This function call faToTwoBit UCSC tool, and return the twoBitFile | 115 This function call faToTwoBit UCSC tool, and return the twoBitFile |
104 :param fasta_file_name: | 116 :param fasta_file_name: |
105 :param mySpecieFolder: | 117 :param mySpecieFolder: |
109 array_call = ['faToTwoBit', fasta_file_name, twoBitFile] | 121 array_call = ['faToTwoBit', fasta_file_name, twoBitFile] |
110 _handleExceptionAndCheckCall(array_call) | 122 _handleExceptionAndCheckCall(array_call) |
111 | 123 |
112 return twoBitFile | 124 return twoBitFile |
113 | 125 |
126 | |
114 def gtfToGenePred(input_gtf_file_name, gene_pred_file_name): | 127 def gtfToGenePred(input_gtf_file_name, gene_pred_file_name): |
115 """ | 128 """ |
116 Call gtfToGenePred and write the result into gene_pred_file_name | 129 Call gtfToGenePred and write the result into gene_pred_file_name |
117 :param input_gtf_file_name: | 130 :param input_gtf_file_name: |
118 :param gene_pred_file_name: | 131 :param gene_pred_file_name: |
120 """ | 133 """ |
121 array_call = ['gtfToGenePred', input_gtf_file_name, gene_pred_file_name] | 134 array_call = ['gtfToGenePred', input_gtf_file_name, gene_pred_file_name] |
122 p = _handleExceptionAndCheckCall(array_call) | 135 p = _handleExceptionAndCheckCall(array_call) |
123 return p | 136 return p |
124 | 137 |
138 | |
125 def gff3ToGenePred(input_gff3_file_name, gene_pred_file_name): | 139 def gff3ToGenePred(input_gff3_file_name, gene_pred_file_name): |
126 """ | 140 """ |
127 Call gff3ToGenePred and write the result into gene_pred_file_name | 141 Call gff3ToGenePred and write the result into gene_pred_file_name |
128 :param input_gff3_file_name: | 142 :param input_gff3_file_name: |
129 :param gene_pred_file_name: | 143 :param gene_pred_file_name: |
130 :return: | 144 :return: |
131 """ | 145 """ |
132 valid_gff3_file = tempfile.NamedTemporaryFile(bufsize=0, suffix=".gff3") | 146 array_call = ['gff3ToGenePred', input_gff3_file_name, gene_pred_file_name] |
133 validateGff(input_gff3_file_name, valid_gff3_file.name) | 147 p = _handleExceptionAndCheckCall(array_call) |
134 array_call = ['gff3ToGenePred', valid_gff3_file.name, gene_pred_file_name] | 148 return p |
135 p = _handleExceptionAndCheckCall(array_call) | 149 |
136 return p | |
137 | 150 |
138 def genePredToBigGenePred(gene_pred_file_name, unsorted_bigGenePred_file_name): | 151 def genePredToBigGenePred(gene_pred_file_name, unsorted_bigGenePred_file_name): |
139 """ | 152 """ |
140 Call genePredToBigGenePred and write the result into unsorted_bigGenePred_file_name | 153 Call genePredToBigGenePred and write the result into unsorted_bigGenePred_file_name |
141 :param gene_pred_file_name: | 154 :param gene_pred_file_name: |
146 gene_pred_file_name, | 159 gene_pred_file_name, |
147 unsorted_bigGenePred_file_name] | 160 unsorted_bigGenePred_file_name] |
148 p = _handleExceptionAndCheckCall(array_call) | 161 p = _handleExceptionAndCheckCall(array_call) |
149 return p | 162 return p |
150 | 163 |
164 | |
151 def genePredToBed(gene_pred_file_name, unsorted_bed_file_name): | 165 def genePredToBed(gene_pred_file_name, unsorted_bed_file_name): |
152 """ | 166 """ |
153 Call genePredToBed and write the result into unsorted_bed_file_name | 167 Call genePredToBed and write the result into unsorted_bed_file_name |
154 :param gene_pred_file_name: | 168 :param gene_pred_file_name: |
155 :param unsorted_bed_file_name: | 169 :param unsorted_bed_file_name: |
157 """ | 171 """ |
158 array_call = ['genePredToBed', gene_pred_file_name, unsorted_bed_file_name] | 172 array_call = ['genePredToBed', gene_pred_file_name, unsorted_bed_file_name] |
159 p = _handleExceptionAndCheckCall(array_call) | 173 p = _handleExceptionAndCheckCall(array_call) |
160 return p | 174 return p |
161 | 175 |
176 | |
162 def sort(unsorted_bed_file_name, sorted_bed_file_name): | 177 def sort(unsorted_bed_file_name, sorted_bed_file_name): |
163 """ | 178 """ |
164 Call sort with -k1,1 -k2,2n on unsorted_bed_file_name and write the result into sorted_bed_file_name | 179 Call sort with -k1,1 -k2,2n on unsorted_bed_file_name and write the result into sorted_bed_file_name |
165 :param unsorted_bed_file_name: | 180 :param unsorted_bed_file_name: |
166 :param sorted_bed_file_name: | 181 :param sorted_bed_file_name: |
167 :return: | 182 :return: |
168 """ | 183 """ |
169 array_call = ['sort', '-k', '1,1', '-k', '2,2n', unsorted_bed_file_name, '-o', sorted_bed_file_name] | 184 array_call = ['sort', '-k', '1,1', '-k', '2,2n', |
170 p = _handleExceptionAndCheckCall(array_call) | 185 unsorted_bed_file_name, '-o', sorted_bed_file_name] |
171 return p | 186 p = _handleExceptionAndCheckCall(array_call) |
187 return p | |
188 | |
172 | 189 |
173 def sortChromSizes(two_bit_info_file_name, chrom_sizes_file_name): | 190 def sortChromSizes(two_bit_info_file_name, chrom_sizes_file_name): |
174 """ | 191 """ |
175 Call sort with -k2rn on two_bit_info_file_name and write the result into chrom_sizes_file_name | 192 Call sort with -k2rn on two_bit_info_file_name and write the result into chrom_sizes_file_name |
176 :param two_bit_info_file_name: | 193 :param two_bit_info_file_name: |
177 :param chrom_sizes_file_name: | 194 :param chrom_sizes_file_name: |
178 :return: | 195 :return: |
179 """ | 196 """ |
180 array_call = ['sort', '-k2rn', two_bit_info_file_name, '-o', chrom_sizes_file_name] | 197 array_call = ['sort', '-k2rn', two_bit_info_file_name, |
181 p = _handleExceptionAndCheckCall(array_call) | 198 '-o', chrom_sizes_file_name] |
182 return p | 199 p = _handleExceptionAndCheckCall(array_call) |
183 | 200 return p |
184 def bedToBigBed(sorted_bed_file_name, chrom_sizes_file_name, big_bed_file_name, | 201 |
185 typeOption=None, autoSql=None, tab=False, extraIndex=None): | 202 |
203 def bedToBigBed(sorted_bed_file_name, chrom_sizes_file_name, big_bed_file_name, options=None): | |
186 """ | 204 """ |
187 Call bedToBigBed on sorted_bed_file_name, using chrom_sizes_file_name and write the result into big_bed_file_name | 205 Call bedToBigBed on sorted_bed_file_name, using chrom_sizes_file_name and write the result into big_bed_file_name |
188 :param sorted_bed_file_name: | 206 :param sorted_bed_file_name: |
189 :param chrom_sizes_file_name: | 207 :param chrom_sizes_file_name: |
190 :param big_bed_file_name: | 208 :param big_bed_file_name: |
191 :return: | 209 :return: |
192 """ | 210 """ |
193 | 211 |
194 # TODO: Move this into the _handleExceptionAndCheckCall function | 212 array_call = ['bedToBigBed', sorted_bed_file_name, |
195 # Parse the array | 213 chrom_sizes_file_name, big_bed_file_name] |
196 logging.debug("sorted_bed_file_name: {0}".format(sorted_bed_file_name)) | 214 if options: |
197 logging.debug("chrom_sizes_file_name: {0}".format(chrom_sizes_file_name)) | 215 typeOption = options.get("typeOption") |
198 logging.debug("big_bed_file_name: {0}".format(big_bed_file_name)) | 216 autoSql = options.get("autoSql") |
199 logging.debug("typeOption: {0}".format(typeOption)) | 217 tab = options.get("tab") |
200 logging.debug("autoSql: {0}".format(autoSql)) | 218 extraIndex = options.get("extraIndex") |
201 logging.debug("tab option: {0}".format(tab)) | 219 if typeOption: |
202 | 220 typeOption = ''.join(['-type=', typeOption]) |
203 array_call = ['bedToBigBed', sorted_bed_file_name, chrom_sizes_file_name, big_bed_file_name] | 221 array_call.append(typeOption) |
204 if typeOption: | 222 if autoSql: |
205 typeOption = ''.join(['-type=', typeOption]) | 223 autoSql = ''.join(['-as=', autoSql]) |
206 array_call.append(typeOption) | 224 array_call.append(autoSql) |
207 if autoSql: | 225 if tab: |
208 autoSql = ''.join(['-as=', autoSql]) | 226 array_call.append('-tab') |
209 array_call.append(autoSql) | 227 if extraIndex: |
210 if tab: | 228 index = ''.join(['-extraIndex=', extraIndex]) |
211 array_call.append('-tab') | 229 array_call.append(index) |
212 if extraIndex: | |
213 index = ''.join(['-extraIndex=', extraIndex]) | |
214 array_call.append(index) | |
215 | |
216 p = _handleExceptionAndCheckCall(array_call) | 230 p = _handleExceptionAndCheckCall(array_call) |
217 return p | 231 return p |
218 | 232 |
219 def sortBam(input_bam_file_name, output_sorted_bam_name): | 233 def sortBam(input_bam_file_name, output_sorted_bam_name): |
220 """ | 234 """ |
221 Call samtools on input_bam_file_name and output the result in output_sorted_bam_name | 235 Call samtools on input_bam_file_name and output the result in output_sorted_bam_name |
222 :param input_bam_file_name: | 236 :param input_bam_file_name: |
223 :param output_sorted_bam_name: | 237 :param output_sorted_bam_name: |
224 :return: | 238 :return: |
225 """ | 239 """ |
226 array_call = ['samtools', 'sort', input_bam_file_name, '-o', output_sorted_bam_name] | 240 array_call = ['samtools', 'sort', |
227 p = _handleExceptionAndCheckCall(array_call) | 241 input_bam_file_name, '-o', output_sorted_bam_name] |
228 return p | 242 p = _handleExceptionAndCheckCall(array_call) |
243 return p | |
244 | |
229 | 245 |
230 def createBamIndex(input_sorted_bam_file_name, output_name_index_name): | 246 def createBamIndex(input_sorted_bam_file_name, output_name_index_name): |
231 """ | 247 """ |
232 Call `samtools index` on imput_sorted_bam_file_name and output the result in output_name_index_name | 248 Call `samtools index` on imput_sorted_bam_file_name and output the result in output_name_index_name |
233 :param input_sorted_bam_file_name: | 249 :param input_sorted_bam_file_name: |
234 :param output_name_index_name: | 250 :param output_name_index_name: |
235 :return: | 251 :return: |
236 """ | 252 """ |
237 array_call = ['samtools', 'index', input_sorted_bam_file_name, output_name_index_name] | 253 array_call = ['samtools', 'index', |
238 p = _handleExceptionAndCheckCall(array_call) | 254 input_sorted_bam_file_name, output_name_index_name] |
239 return p | 255 p = _handleExceptionAndCheckCall(array_call) |
256 return p | |
257 | |
240 | 258 |
241 def pslToBigPsl(input_psl_file_name, output_bed12_file_name): | 259 def pslToBigPsl(input_psl_file_name, output_bed12_file_name): |
242 """ | 260 """ |
243 Call `pslToBigPsl` on input_psl_file_name and output the result in output_bed12_file_name | 261 Call `pslToBigPsl` on input_psl_file_name and output the result in output_bed12_file_name |
244 :param input_psl_file_name: Name of the psl input file | 262 :param input_psl_file_name: Name of the psl input file |
249 array_call = ['pslToBigPsl', input_psl_file_name, output_bed12_file_name] | 267 array_call = ['pslToBigPsl', input_psl_file_name, output_bed12_file_name] |
250 | 268 |
251 p = _handleExceptionAndCheckCall(array_call) | 269 p = _handleExceptionAndCheckCall(array_call) |
252 return p | 270 return p |
253 | 271 |
254 #santitize trackName. Because track name must begin with a letter and | 272 # santitize trackName. Because track name must begin with a letter and |
255 # contain only the following chars: [a-zA-Z0-9_]. | 273 # contain only the following chars: [a-zA-Z0-9_]. |
256 # See the "track" Common settings at: | 274 # See the "track" Common settings at: |
257 #https://genome.ucsc.edu/goldenpath/help/trackDb/trackDbHub.html#bigPsl_-_Pairwise_Alignments | 275 # https://genome.ucsc.edu/goldenpath/help/trackDb/trackDbHub.html#bigPsl_-_Pairwise_Alignments |
258 def fixName(filename): | 276 |
259 if filename == 'cytoBandIdeo': | 277 def validateFiles(input_file, chrom_sizes_file_name, file_type, options=None): |
260 return filename | 278 """ |
261 valid_chars = "_%s%s" % (string.ascii_letters, string.digits) | 279 Call validateFiles on input_file, using chrom_sizes_file_name and file_type |
262 sanitize_name = ''.join([c if c in valid_chars else '_' for c in filename]) | 280 :param input_file: |
263 sanitize_name = "gonramp_" + sanitize_name | 281 :param chrom_sizes_file_name: |
264 return sanitize_name | 282 :param file_type: |
265 | 283 :return: |
266 def validateGff(orig_gff3, valid_gff3): | 284 """ |
267 """ | 285 |
268 Remove extra meta line: ##gff-version 3 | 286 array_call = ['validateFiles', '-chromInfo=' + chrom_sizes_file_name, '-type='+ file_type, input_file] |
269 """ | 287 if options: |
270 valid = open(valid_gff3, 'w') | 288 tab = options.get("tab") |
271 num = 0 | 289 autoSql = options.get("autoSql") |
272 with open(orig_gff3, 'r') as f: | 290 logging.debug("tab: {0}".format(tab)) |
273 for line in f: | 291 logging.debug("autoSql: {0}".format(autoSql)) |
274 if '##gff-version 3' in line: | 292 if autoSql: |
275 if num == 0: | 293 autoSql = ''.join(['-as=', autoSql]) |
276 num += 1 | 294 array_call.append(autoSql) |
277 else: | 295 if tab: |
278 continue | 296 array_call.append('-tab') |
279 valid.write(line) | 297 p = _handleExceptionAndCheckCall(array_call) |
280 | 298 return p |
299 | |
300 def pslCheck(input_file, options=None): | |
301 """ | |
302 Call pslCheck on input_file | |
303 :param input_file: | |
304 :return: | |
305 """ | |
306 | |
307 array_call = ['pslCheck', input_file] | |
308 p = _handleExceptionAndCheckCall(array_call) | |
309 return p | |
310 | |
311 | |
312 |