# HG changeset patch # User rnateam # Date 1393068062 18000 # Node ID 6ae946589f04bf358bb65c2e5669dfb30f6e7036 initial Uploaded diff -r 000000000000 -r 6ae946589f04 blockbuster.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/blockbuster.xml Sat Feb 22 06:21:02 2014 -0500 @@ -0,0 +1,59 @@ + + detects blocks of overlapping reads using a gaussian-distribution approach + + blockbuster + + + blockbuster.x + -distance $distance + -minClusterHeight $minClusterHeight + -minBlockHeight $minBlockHeight + -scale $scale + -merge $merge + -tagFilter $tagFilter + -print $print + $input + + > $output + + + + + + + + + + + + + + + + + + + + +.. class:: infomark + +**What it does** + +Blockbuster_ detects blocks of overlapping reads using a gaussian-distribution approach. + + +Once short read sequences are mapped to a reference genome, one will face the problem of dividing consecutive reads into blocks to detect specific expression patterns. Due to biological variability and sequencing inaccuracies, the read arrangement does not always show exact block boundaries. The blockbuster tool automatically assigns reads to blocks and gives a unique chance to actually see the different origins where the short reads come from. + +.. _Blockbuster: http://hoffmann.bioinf.uni-leipzig.de/LIFE/blockbuster.html + + +**References** + + +If you use this program in your work you might want to cite: + +Langenberger D, Bermudez-Santana C, Hertel J, Hoffmann S, Khaitovitch P, Stadler PF: "Evidence for Human microRNA-Offset RNAs in Small RNA Sequencing Data", Bioinformatics (2009) vol. 25 (18) pp. 2298-301 + + + + diff -r 000000000000 -r 6ae946589f04 tool_dependencies.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Sat Feb 22 06:21:02 2014 -0500 @@ -0,0 +1,24 @@ + + + + + + + https://github.com/bgruening/download_store/raw/master/blockbuster/blockbuster-0.0.1.tar.gz + make + + blockbuster.x + $INSTALL_DIR/bin + + + $INSTALL_DIR/bin + + + + + Blockbuster detects blocks of overlapping reads using a gaussian-distribution approach. + GCC is needed to compilte Blockbuster. + http://hoffmann.bioinf.uni-leipzig.de/LIFE/blockbuster.html + + +