Mercurial > repos > rnateam > blockclust
diff blockclust.xml @ 0:768a67f6ae18 draft
Uploaded
| author | rnateam |
|---|---|
| date | Tue, 06 May 2014 07:49:25 -0400 |
| parents | |
| children | a92d8689aa30 |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/blockclust.xml Tue May 06 07:49:25 2014 -0400 @@ -0,0 +1,186 @@ +<tool id="blockclust" name="BlockClust" version="1.0.0"> + <description>Non-coding RNA clustering from deep sequencing read profiles</description> + <requirements> + <requirement type="package" version="1.0">blockclust</requirement> + <requirement type="package" version="1.1">eden</requirement> + <requirement type="package" version="3.0.1">R_3_0_1</requirement> + <requirement type="package" version="0.1.19">samtools</requirement> + <requirement type="package" version="12.135">mcl</requirement> + <requirement type="package" version="1.0">blockclust_rlibs</requirement> + </requirements> + <version_command>echo '1.0'</version_command> + <command> + + #if str($tool_mode.operation) == "pre": + #set $outputdir = $tags_bed.extra_files_path + BlockClustPipeLine.pl -m PRE -bam $tool_mode.reads_bam -tbed $tags_bed + #elif str($tool_mode.operation) == "clust": + #set $outputdir = $clusters.extra_files_path + #set $accept_bed=list() + #set $reject_bed=list() + ## prepare annotations + #if str($tool_mode.reference) == "hg19": + $accept_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/hg19/hg19.accept.bed") + $reject_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/hg19/hg19.reject.bed") + #elif str($tool_mode.reference) == "mm10": + $accept_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/mm10/mm10.accept.bed") + $reject_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/mm10/mm10.reject.bed") + #elif str($tool_mode.reference) == "dm3": + $accept_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/dm3/dm3.accept.bed") + $reject_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/dm3/dm3.reject.bed") + #elif str($tool_mode.reference) == "rheMac3": + $accept_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/rheMac3/rheMac3.accept.bed") + $reject_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/rheMac3/rheMac3.reject.bed") + #elif str($tool_mode.reference) == "panTro4": + $accept_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/panTro3/panTro4.accept.bed") + $reject_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/panTro3/panTro4.reject.bed") + #elif str($tool_mode.reference) == "xenTro3": + $accept_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/xenTro3/xenTro3.accept.bed") + $reject_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/xenTro3/xenTro3.reject.bed") + #elif str($tool_mode.reference) == "celWS235": + $accept_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/celWS235/celWS235.accept.bed") + $reject_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/celWS235/celWS235.reject.bed") + #elif str($tool_mode.reference) == "tair10": + $accept_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/tair10/tair10.accept.bed") + $reject_bed.append("\$BLOCKCLUST_DATA_PATH/annotations/tair10/tair10.reject.bed") + #end if + BlockClustPipeLine.pl -m TEST -f SEQUENCE -c \$BLOCKCLUST_DATA_PATH/blockclust.config + -t $tool_mode.input_bbo + -a #echo ''.join( $accept_bed ) + -r #echo ''.join( $reject_bed ) + -o $outputdir + #if str($tool_mode.pred.enable_pred) == "yes": + -p + -pm $tool_mode.pred.pred_mode + -md \$BLOCKCLUST_DATA_PATH/models + -x 3; + #if str($tool_mode.pred.pred_mode) == "nearest_neighbour": + cp #echo os.path.join($outputdir,'nearest_neighbour_predictions.txt')# $nearest_neighbour_pred_bed; + #elif str($tool_mode.pred.pred_mode) == "model_based": + cp #echo os.path.join($outputdir,'model_based_predictions.txt')# $model_based_pred_bed; + #end if + #else: + ; + #end if + + cp #echo os.path.join($outputdir, 'mcl_clusters','all_clusters.bed')# $clusters; + cp #echo os.path.join($outputdir, 'hclust_tree.pdf')# $hclust_plot; + cp #echo os.path.join($outputdir, 'discretized.gspan.tab')# $sim_tab_out + #elif str($tool_mode.operation) == "post": + #set $outputdir = $clusters_bed.extra_files_path + BlockClustPipeLine.pl -m POST -cbed $tool_mode.clusters_bed -cm $tool_mode.cmsearch_out -tab $tool_mode.sim_tab_in -o $outputdir; + cp #echo os.path.join($outputdir, 'cluster_distribution.pdf')# $cluster_dist; + cp #echo os.path.join($outputdir, 'hclust_tree_clusters.pdf')# $cluster_hclust + #end if + </command> + <inputs> + <conditional name="tool_mode"> + <param name="operation" type="select" label="Select mode of operation"> + <option value="pre">Pre-processing </option> + <option value="clust">Clustering</option> + <option value="post">Post-processing</option> + </param> + <when value="pre"> + <param name="reads_bam" type="data" format="bam" label="BAM file containing alignments" /> + </when> + <when value="clust"> + <param name="input_bbo" type="data" format="tabular" label="Input blockgroups file" /> + <param name="reference" type="select" label="Select reference genome"> + <option value="hg19">Human (hg19)</option> + <option value="mm10">Mouse (mm10)</option> + <option value="dm3">Fly (dm3)</option> + <option value="rheMac3">Monkey (rheMac3)</option> + <option value="panTro4">Chimp (panTro4)</option> + <option value="xenTro3">Frog (xenTro3)</option> + <option value="celWS235">C. elegans (celWS235)</option> + <option value="tair10">Arabidopsis thaliana (tair10)</option> + </param> + <conditional name="pred"> + <param name="enable_pred" type="select" label="Would you like to perform classification?"> + <option value="no">No</option> + <option value="yes">Yes</option> + </param> + <when value="yes"> + <param name="pred_mode" type="select" label="Mode of classification"> + <option value="model_based">Model based</option> + <option value="nearest_neighbour">Nearest neighbour</option> + </param> + </when> + </conditional> + </when> + <when value="post"> + <param name="cmsearch_out" type="data" format="tabular" label="Output of cmsearch tool" /> + <param name="clusters_bed" type="data" format="bed" label="BED file containing clusters (output of BlockClust)" /> + <param name="sim_tab_in" type="data" format="tabular" label="Pairwise similarities file" /> + </when> + </conditional> + </inputs> + + <outputs> + <data format="bed" name="tags_bed" label="BlockClust: BAM to BED on ${on_string}"> + <filter> tool_mode["operation"]=="pre"</filter> + </data> + <data format="pdf" name="hclust_plot" label="BlockClust: Hierarchical clustering plot on ${on_string}" > + <filter> tool_mode["operation"]=="clust"</filter> + </data> + <data format="bed" name="clusters" label="BlockClust: Clustering BED on ${on_string}"> + <filter> tool_mode["operation"]=="clust"</filter> + </data> + <data format="bed" name="model_based_pred_bed" label="BlockClust: Model based prediction BED on ${on_string}"> + <filter> + (( + tool_mode["operation"] == 'clust' and + tool_mode["pred"]["enable_pred"] == "yes" and + tool_mode["pred"]["pred_mode"] == "model_based" + )) + </filter> + </data> + <data format="bed" name="nearest_neighbour_pred_bed" label="BlockClust: Nearest neighbour prediction BED on ${on_string}"> + <filter> + (( + tool_mode["operation"] == 'clust' and + tool_mode["pred"]["enable_pred"] == "yes" and + tool_mode["pred"]["pred_mode"] == "nearest_neighbour" + )) + </filter> + </data> + <data format="tabular" name="sim_tab_out" label="BlockClust: Pairwise similarities on ${on_string}"> + <filter> tool_mode["operation"]=="clust"</filter> + </data> + <data format="pdf" name="cluster_dist" label="BlockClust: Cluster distribution on ${on_string}" > + <filter> tool_mode["operation"]=="post"</filter> + </data> + <data format="pdf" name="cluster_hclust" label="BlockClust: Hierarchical clustering plot of cluster centroids on ${on_string}" > + <filter> tool_mode["operation"]=="post"</filter> + </data> + </outputs> + <help> + +.. class:: infomark + +**What it does** + +Clusters the read profiles (i.e., blockgroups) from the blockbuster tool. + +**Inputs** + +BlockClust needs output of tool blockbuster as input + +**Output** + +BlockClust produces a fasta file containing clusters. + +------ + +**Licenses** + +If **BlockClust** is used to obtain results for scientific publications it +should be cited as [1]_. + +**References** + +.. [1] + + + </help> +</tool>