Mercurial > repos > rnateam > chipseeker
diff chipseeker.R @ 6:b418a1d3585d draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chipseeker commit d83142dbe2432fcb0f56dcd6311a05c061628ecc
author | rnateam |
---|---|
date | Wed, 13 Mar 2019 03:05:16 -0400 |
parents | 90fe78a19b55 |
children | 1b9a9409831d |
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--- a/chipseeker.R Tue Dec 04 10:01:20 2018 -0500 +++ b/chipseeker.R Wed Mar 13 03:05:16 2019 -0400 @@ -17,6 +17,8 @@ make_option(c("-d","--downstream"), type="integer", help="TSS downstream region"), make_option(c("-F","--flankgeneinfo"), type="logical", help="Add flanking gene info"), make_option(c("-D","--flankgenedist"), type="integer", help="Flanking gene distance"), + make_option(c("-j","--ignoreUpstream"), type="logical", help="Ignore upstream"), + make_option(c("-k","--ignoreDownstream"), type="logical", help="Ignore downstream"), make_option(c("-f","--format"), type="character", help="Output format (interval or tabular)."), make_option(c("-p","--plots"), type="logical", help="PDF of plots."), make_option(c("-r","--rdata"), type="logical", help="Output RData file.") @@ -31,15 +33,36 @@ down = args$downstream format = args$format +if (!is.null(args$flankgeneinfo)) { + flankgeneinfo <- TRUE +} else { + flankgeneinfo <- FALSE +} + +if (!is.null(args$ignoreUpstream)) { + ignoreUpstream <- TRUE +} else { + ignoreUpstream <- FALSE +} + +if (!is.null(args$ignoreDownstream)) { + ignoreDownstream <- TRUE +} else { + ignoreDownstream <- FALSE +} + peaks <- readPeakFile(peaks) # Make TxDb from GTF txdb <- makeTxDbFromGFF(gtf, format="gtf") -if (!is.null(args$flankgeneinfo)) { - peakAnno <- annotatePeak(peaks, TxDb=txdb, tssRegion=c(-up, down), addFlankGeneInfo=args$flankgeneinfo, flankDistance=args$flankgenedist) -} else { - peakAnno <- annotatePeak(peaks, TxDb=txdb, tssRegion=c(-up, down)) -} + +# Annotate peaks +peakAnno <- annotatePeak(peaks, TxDb=txdb, + tssRegion=c(-up, down), + addFlankGeneInfo=flankgeneinfo, + flankDistance=args$flankgenedist, + ignoreUpstream=ignoreUpstream, + ignoreDownstream=ignoreDownstream) # Add gene name features <- import(gtf, format="gtf")