comparison gplib.py @ 5:ddcf35a868b8 draft default tip

planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/graphprot commit ad60258f5759eaa205fec4af6143c728ea131419

4:4ad83aed5c3c

import gzip
import random
import re
import statistics
import subprocess
from distutils.spawn import find_executable


"""

Run doctests:

python3 -m doctest gplib.py


"""


###############################################################################

def graphprot_predictions_get_median(predictions_file):
    """
    Given a GraphProt .predictions file, read in site scores and return
    the median value.

    f.close()
    # Return the median.
    return statistics.median(sc_list)


###############################################################################

def graphprot_profile_get_tsm(profile_file,
                              profile_type="profile",
                              avg_profile_extlr=5):

    """
    Given a GraphProt .profile file, extract for each site (identified by
    column 1 ID) the top (= highest) score. Then return the median of these
    top scores.

        if profile_type == "profile":
            max_sc = max(lists_dic[seq_id])
            max_list.append(max_sc)
        elif profile_type == "avg_profile":
            # Convert profile score list to average profile scores list.
            aps_list = \
                list_moving_window_average_values(lists_dic[seq_id],
                                                  win_extlr=avg_profile_extlr)
            max_sc = max(aps_list)
            max_list.append(max_sc)
        else:
            assert 0, "invalid profile_type argument given: \"%s\"" \
                % (profile_type)
    # Return the median.
    return statistics.median(max_list)


###############################################################################

def list_moving_window_average_values(in_list,
                                      win_extlr=5,
                                      method=1):
    """
    Take a list of numeric values, and calculate for each position a new value,
    by taking the mean value of the window of positions -win_extlr and
    +win_extlr. If full extension is not possible (at list ends), it just
    takes what it gets.

    else:
        assert 0, "invalid method ID given (%i)" % (method)
    return new_list


###############################################################################

def echo_add_to_file(echo_string, out_file):
    """
    Add a string to file, using echo command.

    if output:
        error = True
    assert not error, "echo is complaining:\n%s\n%s" % (check_cmd, output)


###############################################################################

def is_tool(name):
    """Check whether tool "name" is in PATH."""
    return find_executable(name) is not None


###############################################################################

def count_fasta_headers(fasta_file):
    """
    Count number of FASTA headers in fasta_file using grep.

    output = subprocess.getoutput(check_cmd)
    row_count = int(output.strip())
    return row_count


###############################################################################

def make_file_copy(in_file, out_file):
    """
    Make a file copy by copying in_file to out_file.

    """
    check_cmd = "cat " + in_file + " > " + out_file
    assert in_file != out_file, \
        "cat does not like to cat file into same file (%s)" % (check_cmd)
    output = subprocess.getoutput(check_cmd)
    error = False
    if output:
        error = True
    assert not error, \
        "cat did not like your input (in_file: %s, out_file: %s):\n%s" \
        % (in_file, out_file, output)


###############################################################################

def split_fasta_into_test_train_files(in_fasta, test_out_fa, train_out_fa,
                                      test_size=500):
    """
    Split in_fasta .fa file into two files (e.g. test, train).

    """
    # Read in in_fasta.

    c_out = 0
    TESTOUT = open(test_out_fa, "w")
    TRAINOUT = open(train_out_fa, "w")
    for seq_id in rand_ids_list:
        seq = seqs_dic[seq_id]
        if (c_out >= test_size):
            TRAINOUT.write(">%s\n%s\n" % (seq_id, seq))
        else:
            TESTOUT.write(">%s\n%s\n" % (seq_id, seq))
        c_out += 1
    TESTOUT.close()
    TRAINOUT.close()


###############################################################################

def check_seqs_dic_format(seqs_dic):
    """
    Check sequence dictionary for lowercase-only sequences or sequences
    wich have lowercase nts in between uppercase nts.

        if re.search("[ACGTUN][acgtun]+[ACGTUN]", seq):
            bad_seq_ids.append(seq_id)
    return bad_seq_ids


###############################################################################

def read_fasta_into_dic(fasta_file,
                        seqs_dic=False,
                        ids_dic=False,
                        read_dna=False,
                        short_ensembl=False,
                        reject_lc=False,
                        convert_to_uc=False,
                        skip_n_seqs=True):
    """
    Read in FASTA sequences, convert to RNA, store in dictionary
    and return dictionary.

    >>> test_fasta = "test-data/test.fa"

    seq_id = ""
    seq = ""

    # Go through FASTA file, extract sequences.
    if re.search(r".+\.gz$", fasta_file):
        f = gzip.open(fasta_file, 'rt')
    else:
        f = open(fasta_file, "r")
    for line in f:
        if re.search(">.+", line):
            m = re.search(">(.+)", line)

            # This assumes ENSEMBL header format ">ENST00000631435.1 cdna ..."
            if short_ensembl:
                if re.search(r".+\..+", seq_id):
                    m = re.search(r"(.+?)\..+", seq_id)
                    seq_id = m.group(1)
            assert seq_id not in seqs_dic, \
                "non-unique FASTA header \"%s\" in \"%s\"" \
                % (seq_id, fasta_file)
            if ids_dic:
                if seq_id in ids_dic:
                    seqs_dic[seq_id] = ""
            else:
                seqs_dic[seq_id] = ""
        elif re.search("[ACGTUN]+", line, re.I):
            if seq_id in seqs_dic:
                m = re.search("([ACGTUN]+)", line, re.I)
                seq = m.group(1)
                if reject_lc:
                    assert \
                        not re.search("[a-z]", seq), \
                        "lc char detected in seq \"%i\" (reject_lc=True)" \
                        % (seq_id)
                if convert_to_uc:
                    seq = seq.upper()
                # If sequences with N nucleotides should be skipped.
                if skip_n_seqs:
                    if "n" in m.group(1) or "N" in m.group(1):
                        print("WARNING: \"%s\" contains N. Discarding "
                              "sequence ... " % (seq_id))
                        del seqs_dic[seq_id]
                        continue
                # Convert to RNA, concatenate sequence.
                if read_dna:
                    seqs_dic[seq_id] += \
                        m.group(1).replace("U", "T").replace("u", "t")
                else:
                    seqs_dic[seq_id] += \
                        m.group(1).replace("T", "U").replace("t", "u")
    f.close()
    return seqs_dic


###############################################################################

def random_order_dic_keys_into_list(in_dic):
    """
    Read in dictionary keys, and return random order list of IDs.

        id_list.append(key)
    random.shuffle(id_list)
    return id_list


###############################################################################

def graphprot_get_param_string(params_file):
    """
    Get parameter string from GraphProt .params file.

                    if setting == "sequence":
                        param_string += "-onlyseq "
                else:
                    param_string += "-%s %s " % (par, setting)
            else:
                assert 0, "pattern matching failed for string \"%s\"" % (param)
    return param_string


###############################################################################

def seqs_dic_count_uc_nts(seqs_dic):
    """
    Count number of uppercase nucleotides in sequences stored in sequence
    dictionary.

    """
    assert seqs_dic, "Given sequence dictionary empty"
    c_uc = 0
    for seq_id in seqs_dic:
        c_uc += len(re.findall(r'[A-Z]', seqs_dic[seq_id]))
    return c_uc


###############################################################################

def seqs_dic_count_lc_nts(seqs_dic):
    """
    Count number of lowercase nucleotides in sequences stored in sequence
    dictionary.

    """
    assert seqs_dic, "Given sequence dictionary empty"
    c_uc = 0
    for seq_id in seqs_dic:
        c_uc += len(re.findall(r'[a-z]', seqs_dic[seq_id]))
    return c_uc


###############################################################################

def count_file_rows(in_file):
    """
    Count number of file rows for given input file.

    output = subprocess.getoutput(check_cmd)
    row_count = int(output.strip())
    return row_count


###############################################################################

def bed_check_six_col_format(bed_file):
    """
    Check whether given .bed file has 6 columns.

            break
    f.closed
    return six_col_format


###############################################################################

def bed_check_unique_ids(bed_file):
    """
    Check whether .bed file (6 column format with IDs in column 4)
    has unique column 4 IDs.

        return False
    else:
        return True


###############################################################################

def get_seq_lengths_from_seqs_dic(seqs_dic):
    """
    Given a dictionary of sequences, return dictionary of sequence lengths.
    Mapping is sequence ID -> sequence length.

        seq_l = len(seqs_dic[seq_id])
        seq_len_dic[seq_id] = seq_l
    return seq_len_dic


###############################################################################

def bed_get_region_lengths(bed_file):
    """
    Read in .bed file, store and return region lengths in dictionary.
    key   :  region ID (.bed col4)

            cols = line.strip().split("\t")
            site_s = int(cols[1])
            site_e = int(cols[2])
            site_id = cols[3]
            site_l = site_e - site_s
            assert site_id \
                not in id2len_dic, \
                "column 4 IDs not unique in given .bed file \"%s\"" \
                % (bed_file)
            id2len_dic[site_id] = site_l
    f.closed
    assert id2len_dic, \
        "No IDs read into dic (input file \"%s\" empty or malformatted?)" \
        % (bed_file)
    return id2len_dic


###############################################################################

def graphprot_get_param_dic(params_file):
    """
    Read in GraphProt .params file and store in dictionary.
    key = parameter

                param_dic[par] = setting
    f.close()
    return param_dic


###############################################################################

def graphprot_filter_predictions_file(in_file, out_file,
                                      sc_thr=0):
    """
    Filter GraphProt .predictions file by given score thr_sc.
    """
    OUTPRED = open(out_file, "w")
    with open(in_file) as f:

            OUTPRED.write("%s\n" % (row))
    f.close()
    OUTPRED.close()


###############################################################################

def fasta_read_in_ids(fasta_file):
    """
    Given a .fa file, read in header IDs in order appearing in file,
    and store in list.

                ids_list.append(seq_id)
    f.close()
    return ids_list


###############################################################################

def graphprot_profile_calc_avg_profile(in_file, out_file,
                                       ap_extlr=5,
                                       seq_ids_list=False,
                                       method=1):
    """
    Given a GraphProt .profile file, calculate average profiles and output
    average profile file.
    Average profile means that the position-wise scores will get smoothed
    out by calculating for each position a new score, taking a sequence

        f.close()
        # Check number of IDs (# FASTA IDs has to be same as # site IDs).
        if seq_ids_list:
            c_seq_ids = len(seq_ids_list)
            c_site_ids = len(site_starts_dic)
            assert c_seq_ids == c_site_ids, \
                "# sequence IDs != # site IDs (%i != %i)" \
                % (c_seq_ids, c_site_ids)
        OUTPROF = open(out_file, "w")
        # For each site, calculate average profile scores list.
        for site_id in lists_dic:
            # Convert profile score list to average profile scores list.
            aps_list = list_moving_window_average_values(lists_dic[site_id],
                                                         win_extlr=ap_extlr)
            start_pos = site_starts_dic[site_id]
            # Get original FASTA sequence ID.
            if seq_ids_list:
                site_id = seq_ids_list[site_id]
            for i, sc in enumerate(aps_list):

                    site_starts_dic[cur_id] = pos
                # Case: new site (new column 1 ID).
                if cur_id != old_id:
                    # Process old id scores.
                    if scores_list:
                        aps_list = \
                            list_moving_window_average_values(
                                scores_list,
                                win_extlr=ap_extlr)
                        start_pos = site_starts_dic[old_id]
                        seq_id = old_id
                        # Get original FASTA sequence ID.
                        if seq_ids_list:
                            seq_id = seq_ids_list[old_id]

                    # Add to scores_list.
                    scores_list.append(score)
        f.close()
        # Process last block.
        if scores_list:
            aps_list = list_moving_window_average_values(scores_list,
                                                         win_extlr=ap_extlr)
            start_pos = site_starts_dic[old_id]
            seq_id = old_id
            # Get original FASTA sequence ID.
            if seq_ids_list:
                seq_id = seq_ids_list[old_id]

                pos = i + start_pos + 1  # make 1-based.
                OUTPROF.write("%s\t%i\t%f\n" % (seq_id, pos, sc))
        OUTPROF.close()


###############################################################################

def graphprot_profile_extract_peak_regions(in_file, out_file,
                                           max_merge_dist=0,
                                           sc_thr=0):
    """
    Extract peak regions from GraphProt .profile file.
    Store the peak regions (defined as regions with scores >= sc_thr)
    as to out_file in 6-column .bed.

            # Case: new site (new column 1 ID).
            if cur_id != old_id:
                # Process old id scores.
                if scores_list:
                    # Extract peaks from region.
                    peak_list = \
                        list_extract_peaks(scores_list,
                                           max_merge_dist=max_merge_dist,
                                           coords="bed",
                                           sc_thr=sc_thr)
                    start_pos = site_starts_dic[old_id]
                    # Print out peaks in .bed format.
                    for ln in peak_list:
                        peak_s = start_pos + ln[0]
                        peak_e = start_pos + ln[1]
                        site_id = "%s,%i" % (old_id, ln[2])
                        OUTPEAKS.write("%s\t%i\t%i"
                                       "\t%s\t%f\t+\n"
                                       % (old_id, peak_s,
                                          peak_e, site_id, ln[3]))
                    # Reset list.
                    scores_list = []
                old_id = cur_id
                scores_list.append(score)
            else:

                scores_list.append(score)
    f.close()
    # Process last block.
    if scores_list:
        # Extract peaks from region.
        peak_list = list_extract_peaks(scores_list,
                                       max_merge_dist=max_merge_dist,
                                       coords="bed",
                                       sc_thr=sc_thr)
        start_pos = site_starts_dic[old_id]
        # Print out peaks in .bed format.
        for ln in peak_list:
            peak_s = start_pos + ln[0]
            peak_e = start_pos + ln[1]
            site_id = "%s,%i" % (old_id, ln[2])  # best score also 1-based.
            OUTPEAKS.write("%s\t%i\t%i\t%s\t%f\t+\n"
                           % (old_id, peak_s, peak_e, site_id, ln[3]))
    OUTPEAKS.close()


###############################################################################

def list_extract_peaks(in_list,
                       max_merge_dist=0,
                       coords="list",
                       sc_thr=0):
    """
    Extract peak regions from list.
    Peak region is defined as region >= score threshold.

    coords=bed  :  peak start 0-based, peak end 1-based.

                    new_top_pos = peak_list[i][2]
                    new_top_sc = peak_list[i][3]
                    if peak_list[i][3] < peak_list[j][3]:
                        new_top_pos = peak_list[j][2]
                        new_top_sc = peak_list[j][3]
                    new_peak = [peak_list[i][0], peak_list[j][1],
                                new_top_pos, new_top_sc]
                # If two peaks were merged.
                if new_peak:
                    merged_peak_list.append(new_peak)
                    added_peaks_dic[i] = 1
                    added_peaks_dic[j] = 1

            peak_list[i][1] += 1
            peak_list[i][2] += 1  # 1-base best score position too.
    return peak_list


###############################################################################

def bed_peaks_to_genomic_peaks(peak_file, genomic_peak_file, genomic_sites_bed,
                               print_rows=False):
    """
    Given a .bed file of sequence peak regions (possible coordinates from
    0 to length of s), convert peak coordinates to genomic coordinates.
    Do this by taking genomic regions of sequences as input.

    with open(genomic_sites_bed) as f:
        for line in f:
            row = line.strip()
            cols = line.strip().split("\t")
            site_id = cols[3]
            assert site_id \
                not in id2row_dic, \
                "column 4 IDs not unique in given .bed file \"%s\"" \
                % (genomic_sites_bed)
            id2row_dic[site_id] = row
    f.close()

    # Read in peaks file and convert coordinates.
    OUTPEAKS = open(genomic_peak_file, "w")

            site_id = cols[0]
            site_s = int(cols[1])
            site_e = int(cols[2])
            site_id2 = cols[3]
            site_sc = float(cols[4])
            assert re.search(".+,.+", site_id2), \
                "regular expression failed for ID \"%s\"" % (site_id2)
            m = re.search(r".+,(\d+)", site_id2)
            sc_pos = int(m.group(1))  # 1-based.
            assert site_id in id2row_dic, \
                "site ID \"%s\" not found in genomic sites dictionary" \
                % (site_id)
            row = id2row_dic[site_id]
            rowl = row.split("\t")
            gen_chr = rowl[0]
            gen_s = int(rowl[1])
            gen_e = int(rowl[2])

            new_sc_pos = sc_pos + gen_s
            if gen_pol == "-":
                new_s = gen_e - site_e
                new_e = gen_e - site_s
                new_sc_pos = gen_e - sc_pos + 1  # keep 1-based.
            new_row = "%s\t%i\t%i\t%s,%i\t%f\t%s" \
                      % (gen_chr, new_s, new_e,
                         site_id, new_sc_pos, site_sc, gen_pol)
            OUTPEAKS.write("%s\n" % (new_row))
            if print_rows:
                print(new_row)
    OUTPEAKS.close()


###############################################################################

def diff_two_files_identical(file1, file2):
    """
    Check whether two files are identical. Return true if diff reports no
    differences.

    if output:
        same = False
    return same


###############################################################################

5:ddcf35a868b8

import gzip
import random
import re
import statistics
import subprocess
from distutils.spawn import find_executable

"""

Run doctests:

python3 -m doctest gplib.py


"""


#######################################################################


def graphprot_predictions_get_median(predictions_file):
    """
    Given a GraphProt .predictions file, read in site scores and return
    the median value.

    f.close()
    # Return the median.
    return statistics.median(sc_list)


#######################################################################


def graphprot_profile_get_tsm(
    profile_file, profile_type="profile", avg_profile_extlr=5
):

    """
    Given a GraphProt .profile file, extract for each site (identified by
    column 1 ID) the top (= highest) score. Then return the median of these
    top scores.

        if profile_type == "profile":
            max_sc = max(lists_dic[seq_id])
            max_list.append(max_sc)
        elif profile_type == "avg_profile":
            # Convert profile score list to average profile scores list.
            aps_list = list_moving_window_average_values(
                lists_dic[seq_id], win_extlr=avg_profile_extlr
            )
            max_sc = max(aps_list)
            max_list.append(max_sc)
        else:
            assert 0, 'invalid profile_type argument given: "%s"' % (profile_type)
    # Return the median.
    return statistics.median(max_list)


#######################################################################


def list_moving_window_average_values(in_list, win_extlr=5, method=1):
    """
    Take a list of numeric values, and calculate for each position a new value,
    by taking the mean value of the window of positions -win_extlr and
    +win_extlr. If full extension is not possible (at list ends), it just
    takes what it gets.

    else:
        assert 0, "invalid method ID given (%i)" % (method)
    return new_list


#######################################################################


def echo_add_to_file(echo_string, out_file):
    """
    Add a string to file, using echo command.

    if output:
        error = True
    assert not error, "echo is complaining:\n%s\n%s" % (check_cmd, output)


#######################################################################


def is_tool(name):
    """Check whether tool "name" is in PATH."""
    return find_executable(name) is not None


#######################################################################


def count_fasta_headers(fasta_file):
    """
    Count number of FASTA headers in fasta_file using grep.

    output = subprocess.getoutput(check_cmd)
    row_count = int(output.strip())
    return row_count


#######################################################################


def make_file_copy(in_file, out_file):
    """
    Make a file copy by copying in_file to out_file.

    """
    check_cmd = "cat " + in_file + " > " + out_file
    assert in_file != out_file, "cat does not like to cat file into same file (%s)" % (
        check_cmd
    )
    output = subprocess.getoutput(check_cmd)
    error = False
    if output:
        error = True
    assert not error, "cat did not like your input (in_file: %s, out_file: %s):\n%s" % (
        in_file,
        out_file,
        output,
    )


#######################################################################


def split_fasta_into_test_train_files(
    in_fasta, test_out_fa, train_out_fa, test_size=500
):
    """
    Split in_fasta .fa file into two files (e.g. test, train).

    """
    # Read in in_fasta.

    c_out = 0
    TESTOUT = open(test_out_fa, "w")
    TRAINOUT = open(train_out_fa, "w")
    for seq_id in rand_ids_list:
        seq = seqs_dic[seq_id]
        if c_out >= test_size:
            TRAINOUT.write(">%s\n%s\n" % (seq_id, seq))
        else:
            TESTOUT.write(">%s\n%s\n" % (seq_id, seq))
        c_out += 1
    TESTOUT.close()
    TRAINOUT.close()


#######################################################################


def check_seqs_dic_format(seqs_dic):
    """
    Check sequence dictionary for lowercase-only sequences or sequences
    wich have lowercase nts in between uppercase nts.

        if re.search("[ACGTUN][acgtun]+[ACGTUN]", seq):
            bad_seq_ids.append(seq_id)
    return bad_seq_ids


#######################################################################


def read_fasta_into_dic(
    fasta_file,
    seqs_dic=False,
    ids_dic=False,
    read_dna=False,
    short_ensembl=False,
    reject_lc=False,
    convert_to_uc=False,
    skip_n_seqs=True,
):
    """
    Read in FASTA sequences, convert to RNA, store in dictionary
    and return dictionary.

    >>> test_fasta = "test-data/test.fa"

    seq_id = ""
    seq = ""

    # Go through FASTA file, extract sequences.
    if re.search(r".+\.gz$", fasta_file):
        f = gzip.open(fasta_file, "rt")
    else:
        f = open(fasta_file, "r")
    for line in f:
        if re.search(">.+", line):
            m = re.search(">(.+)", line)

            # This assumes ENSEMBL header format ">ENST00000631435.1 cdna ..."
            if short_ensembl:
                if re.search(r".+\..+", seq_id):
                    m = re.search(r"(.+?)\..+", seq_id)
                    seq_id = m.group(1)
            assert seq_id not in seqs_dic, 'non-unique FASTA header "%s" in "%s"' % (
                seq_id,
                fasta_file,
            )
            if ids_dic:
                if seq_id in ids_dic:
                    seqs_dic[seq_id] = ""
            else:
                seqs_dic[seq_id] = ""
        elif re.search("[ACGTUN]+", line, re.I):
            if seq_id in seqs_dic:
                m = re.search("([ACGTUN]+)", line, re.I)
                seq = m.group(1)
                if reject_lc:
                    assert not re.search(
                        "[a-z]", seq
                    ), 'lc char detected in seq "%i" (reject_lc=True)' % (seq_id)
                if convert_to_uc:
                    seq = seq.upper()
                # If sequences with N nucleotides should be skipped.
                if skip_n_seqs:
                    if "n" in m.group(1) or "N" in m.group(1):
                        print(
                            'WARNING: "%s" contains N. Discarding '
                            "sequence ... " % (seq_id)
                        )
                        del seqs_dic[seq_id]
                        continue
                # Convert to RNA, concatenate sequence.
                if read_dna:
                    seqs_dic[seq_id] += m.group(1).replace("U", "T").replace("u", "t")
                else:
                    seqs_dic[seq_id] += m.group(1).replace("T", "U").replace("t", "u")
    f.close()
    return seqs_dic


#######################################################################


def random_order_dic_keys_into_list(in_dic):
    """
    Read in dictionary keys, and return random order list of IDs.

        id_list.append(key)
    random.shuffle(id_list)
    return id_list


#######################################################################


def graphprot_get_param_string(params_file):
    """
    Get parameter string from GraphProt .params file.

                    if setting == "sequence":
                        param_string += "-onlyseq "
                else:
                    param_string += "-%s %s " % (par, setting)
            else:
                assert 0, 'pattern matching failed for string "%s"' % (param)
    return param_string


#######################################################################


def seqs_dic_count_uc_nts(seqs_dic):
    """
    Count number of uppercase nucleotides in sequences stored in sequence
    dictionary.

    """
    assert seqs_dic, "Given sequence dictionary empty"
    c_uc = 0
    for seq_id in seqs_dic:
        c_uc += len(re.findall(r"[A-Z]", seqs_dic[seq_id]))
    return c_uc


#######################################################################


def seqs_dic_count_lc_nts(seqs_dic):
    """
    Count number of lowercase nucleotides in sequences stored in sequence
    dictionary.

    """
    assert seqs_dic, "Given sequence dictionary empty"
    c_uc = 0
    for seq_id in seqs_dic:
        c_uc += len(re.findall(r"[a-z]", seqs_dic[seq_id]))
    return c_uc


#######################################################################


def count_file_rows(in_file):
    """
    Count number of file rows for given input file.

    output = subprocess.getoutput(check_cmd)
    row_count = int(output.strip())
    return row_count


#######################################################################


def bed_check_six_col_format(bed_file):
    """
    Check whether given .bed file has 6 columns.

            break
    f.closed
    return six_col_format


#######################################################################


def bed_check_unique_ids(bed_file):
    """
    Check whether .bed file (6 column format with IDs in column 4)
    has unique column 4 IDs.

        return False
    else:
        return True


#######################################################################


def get_seq_lengths_from_seqs_dic(seqs_dic):
    """
    Given a dictionary of sequences, return dictionary of sequence lengths.
    Mapping is sequence ID -> sequence length.

        seq_l = len(seqs_dic[seq_id])
        seq_len_dic[seq_id] = seq_l
    return seq_len_dic


#######################################################################


def bed_get_region_lengths(bed_file):
    """
    Read in .bed file, store and return region lengths in dictionary.
    key   :  region ID (.bed col4)

            cols = line.strip().split("\t")
            site_s = int(cols[1])
            site_e = int(cols[2])
            site_id = cols[3]
            site_l = site_e - site_s
            assert (
                site_id not in id2len_dic
            ), 'column 4 IDs not unique in given .bed file "%s"' % (bed_file)
            id2len_dic[site_id] = site_l
    f.closed
    assert (
        id2len_dic
    ), 'No IDs read into dic (input file "%s" empty or malformatted?)' % (bed_file)
    return id2len_dic


#######################################################################


def graphprot_get_param_dic(params_file):
    """
    Read in GraphProt .params file and store in dictionary.
    key = parameter

                param_dic[par] = setting
    f.close()
    return param_dic


#######################################################################


def graphprot_filter_predictions_file(in_file, out_file, sc_thr=0):
    """
    Filter GraphProt .predictions file by given score thr_sc.
    """
    OUTPRED = open(out_file, "w")
    with open(in_file) as f:

            OUTPRED.write("%s\n" % (row))
    f.close()
    OUTPRED.close()


#######################################################################


def fasta_read_in_ids(fasta_file):
    """
    Given a .fa file, read in header IDs in order appearing in file,
    and store in list.

                ids_list.append(seq_id)
    f.close()
    return ids_list


#######################################################################


def graphprot_profile_calc_avg_profile(
    in_file, out_file, ap_extlr=5, seq_ids_list=False, method=1
):
    """
    Given a GraphProt .profile file, calculate average profiles and output
    average profile file.
    Average profile means that the position-wise scores will get smoothed
    out by calculating for each position a new score, taking a sequence

        f.close()
        # Check number of IDs (# FASTA IDs has to be same as # site IDs).
        if seq_ids_list:
            c_seq_ids = len(seq_ids_list)
            c_site_ids = len(site_starts_dic)
            assert (
                c_seq_ids == c_site_ids
            ), "# sequence IDs != # site IDs (%i != %i)" % (c_seq_ids, c_site_ids)
        OUTPROF = open(out_file, "w")
        # For each site, calculate average profile scores list.
        for site_id in lists_dic:
            # Convert profile score list to average profile scores list.
            aps_list = list_moving_window_average_values(
                lists_dic[site_id], win_extlr=ap_extlr
            )
            start_pos = site_starts_dic[site_id]
            # Get original FASTA sequence ID.
            if seq_ids_list:
                site_id = seq_ids_list[site_id]
            for i, sc in enumerate(aps_list):

                    site_starts_dic[cur_id] = pos
                # Case: new site (new column 1 ID).
                if cur_id != old_id:
                    # Process old id scores.
                    if scores_list:
                        aps_list = list_moving_window_average_values(
                            scores_list, win_extlr=ap_extlr
                        )
                        start_pos = site_starts_dic[old_id]
                        seq_id = old_id
                        # Get original FASTA sequence ID.
                        if seq_ids_list:
                            seq_id = seq_ids_list[old_id]

                    # Add to scores_list.
                    scores_list.append(score)
        f.close()
        # Process last block.
        if scores_list:
            aps_list = list_moving_window_average_values(
                scores_list, win_extlr=ap_extlr
            )
            start_pos = site_starts_dic[old_id]
            seq_id = old_id
            # Get original FASTA sequence ID.
            if seq_ids_list:
                seq_id = seq_ids_list[old_id]

                pos = i + start_pos + 1  # make 1-based.
                OUTPROF.write("%s\t%i\t%f\n" % (seq_id, pos, sc))
        OUTPROF.close()


#######################################################################


def graphprot_profile_extract_peak_regions(
    in_file, out_file, max_merge_dist=0, sc_thr=0
):
    """
    Extract peak regions from GraphProt .profile file.
    Store the peak regions (defined as regions with scores >= sc_thr)
    as to out_file in 6-column .bed.

            # Case: new site (new column 1 ID).
            if cur_id != old_id:
                # Process old id scores.
                if scores_list:
                    # Extract peaks from region.
                    peak_list = list_extract_peaks(
                        scores_list,
                        max_merge_dist=max_merge_dist,
                        coords="bed",
                        sc_thr=sc_thr,
                    )
                    start_pos = site_starts_dic[old_id]
                    # Print out peaks in .bed format.
                    for ln in peak_list:
                        peak_s = start_pos + ln[0]
                        peak_e = start_pos + ln[1]
                        site_id = "%s,%i" % (old_id, ln[2])
                        OUTPEAKS.write(
                            "%s\t%i\t%i"
                            "\t%s\t%f\t+\n" % (old_id, peak_s, peak_e, site_id, ln[3])
                        )
                    # Reset list.
                    scores_list = []
                old_id = cur_id
                scores_list.append(score)
            else:

                scores_list.append(score)
    f.close()
    # Process last block.
    if scores_list:
        # Extract peaks from region.
        peak_list = list_extract_peaks(
            scores_list, max_merge_dist=max_merge_dist, coords="bed", sc_thr=sc_thr
        )
        start_pos = site_starts_dic[old_id]
        # Print out peaks in .bed format.
        for ln in peak_list:
            peak_s = start_pos + ln[0]
            peak_e = start_pos + ln[1]
            site_id = "%s,%i" % (old_id, ln[2])  # best score also 1-based.
            OUTPEAKS.write(
                "%s\t%i\t%i\t%s\t%f\t+\n" % (old_id, peak_s, peak_e, site_id, ln[3])
            )
    OUTPEAKS.close()


#######################################################################


def list_extract_peaks(in_list, max_merge_dist=0, coords="list", sc_thr=0):
    """
    Extract peak regions from list.
    Peak region is defined as region >= score threshold.

    coords=bed  :  peak start 0-based, peak end 1-based.

                    new_top_pos = peak_list[i][2]
                    new_top_sc = peak_list[i][3]
                    if peak_list[i][3] < peak_list[j][3]:
                        new_top_pos = peak_list[j][2]
                        new_top_sc = peak_list[j][3]
                    new_peak = [
                        peak_list[i][0],
                        peak_list[j][1],
                        new_top_pos,
                        new_top_sc,
                    ]
                # If two peaks were merged.
                if new_peak:
                    merged_peak_list.append(new_peak)
                    added_peaks_dic[i] = 1
                    added_peaks_dic[j] = 1

            peak_list[i][1] += 1
            peak_list[i][2] += 1  # 1-base best score position too.
    return peak_list


#######################################################################


def bed_peaks_to_genomic_peaks(
    peak_file, genomic_peak_file, genomic_sites_bed, print_rows=False
):
    """
    Given a .bed file of sequence peak regions (possible coordinates from
    0 to length of s), convert peak coordinates to genomic coordinates.
    Do this by taking genomic regions of sequences as input.

    with open(genomic_sites_bed) as f:
        for line in f:
            row = line.strip()
            cols = line.strip().split("\t")
            site_id = cols[3]
            assert (
                site_id not in id2row_dic
            ), 'column 4 IDs not unique in given .bed file "%s"' % (genomic_sites_bed)
            id2row_dic[site_id] = row
    f.close()

    # Read in peaks file and convert coordinates.
    OUTPEAKS = open(genomic_peak_file, "w")

            site_id = cols[0]
            site_s = int(cols[1])
            site_e = int(cols[2])
            site_id2 = cols[3]
            site_sc = float(cols[4])
            assert re.search(
                ".+,.+", site_id2
            ), 'regular expression failed for ID "%s"' % (site_id2)
            m = re.search(r".+,(\d+)", site_id2)
            sc_pos = int(m.group(1))  # 1-based.
            assert (
                site_id in id2row_dic
            ), 'site ID "%s" not found in genomic sites dictionary' % (site_id)
            row = id2row_dic[site_id]
            rowl = row.split("\t")
            gen_chr = rowl[0]
            gen_s = int(rowl[1])
            gen_e = int(rowl[2])

            new_sc_pos = sc_pos + gen_s
            if gen_pol == "-":
                new_s = gen_e - site_e
                new_e = gen_e - site_s
                new_sc_pos = gen_e - sc_pos + 1  # keep 1-based.
            new_row = "%s\t%i\t%i\t%s,%i\t%f\t%s" % (
                gen_chr,
                new_s,
                new_e,
                site_id,
                new_sc_pos,
                site_sc,
                gen_pol,
            )
            OUTPEAKS.write("%s\n" % (new_row))
            if print_rows:
                print(new_row)
    OUTPEAKS.close()


#######################################################################


def diff_two_files_identical(file1, file2):
    """
    Check whether two files are identical. Return true if diff reports no
    differences.

    if output:
        same = False
    return same


#######################################################################