# HG changeset patch # User rnateam # Date 1482359457 18000 # Node ID a8705df7c57f1af629c3d917eda7cfb148490aa6 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/methylkit commit 14f0b39f64982773ef0367379b915f742eabcc1b diff -r 000000000000 -r a8705df7c57f methylkit.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/methylkit.xml Wed Dec 21 17:30:57 2016 -0500 @@ -0,0 +1,527 @@ + + DNA methylation analysis and annotation + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + bioconductor-methylkit + + + + + + + + + + + + library("methylKit") + + test_files = list() + control_files = list() + + test_ids = list() + control_ids = list() + + #for $i, $s in enumerate( $test_series ) + test_ids[${i}+1] = paste("test ", ${i}+1, sep="") + test_files[${i}+1] = "${s.input.file_name}" + #end for + + #for $i, $s in enumerate( $control_series ) + control_ids[${i}+1] = paste("control ", ${i}+1, sep="") + control_files[${i}+1] = "${s.input.file_name}" + #end for + + input_files = append(test_files, control_files) + sample_ids = append(test_ids, control_ids) + treatment_tag = c(rep.int(1, length(test_ids)), rep.int(0, length(control_ids))) + + myobj=methRead(input_files, sample.id=sample_ids, assembly="${assembly}", + pipeline="${pipeline}", treatment=treatment_tag) + + pdf('output_statistics.pdf') + for (obj in myobj){ + getMethylationStats(obj,plot=TRUE,both.strands=FALSE) + getCoverageStats(obj,plot=TRUE,both.strands=FALSE) + } + devname = dev.off() + + ## unite function + methidh=unite(myobj) + + pdf("output_correlation.pdf") + getCorrelation(object = methidh, plot=TRUE, method = "${correlation}") + devname = dev.off() + + #if $input_type.choice in ["all", "differential_methylation"]: + ## the last two arguments slim, weighted.mean + ## have the redundant counterparts in effect and adjust, + ## so turning them off to avoide the possible conflict. + myDiff = calculateDiffMeth(methidh, overdispersion="${input_type.overdispersion}", + adjust="${input_type.adjust}", effect="${input_type.effect}", test="${input_type.test}", + slim=FALSE, weighted.mean=FALSE) + + bedgraph(myDiff, file.name="output_myDiff.bedgraph", col.name="meth.diff", + unmeth=FALSE, log.transform=FALSE, negative=FALSE, add.on="") + + MethPerChr = diffMethPerChr(myDiff, plot=FALSE, + qvalue.cutoff=${input_type.qvalue_cutoff}, + meth.cutoff=${input_type.meth_cutoff}) + write.table(MethPerChr, sep="\t", row.names=FALSE, quote=FALSE, file="output_MethPerChr.tsv") + + MethylDiff = getMethylDiff(myDiff, difference=${input_type.meth_cutoff}, + qvalue=${input_type.qvalue_cutoff}, type="${input_type.type}") + bedgraph(MethylDiff, file.name="output_MethylDiff.bedgraph", col.name="meth.diff", + unmeth=FALSE,log.transform=FALSE,negative=FALSE,add.on="") + #end if + + #if $input_type.choice in ["all", "clustering"]: + pdf( "output_clustering.pdf" ) + methClust = clusterSamples(methidh, dist="${input_type.dist}", method="${input_type.method}") + devname = dev.off() + + pdf( "output_PCA.pdf" ) + PCASamples(methidh) + devname = dev.off() + #end if + + #if $input_type.choice in ["all", "segmentation"]: + ## methSeg works for methylRaw or methylDiff with resolution region, + ## so methylBase has to be tiled before + tileRaw = tileMethylCounts(myobj[[1]]) + tileBase = tileMethylCounts(methidh) + tileDiff = calculateDiffMeth(tileBase) + + ## methseg generates Granges + segRaw = methSeg(tileRaw, diagnostic.plot = FALSE) + segDiff = methSeg(tileDiff, diagnostic.plot = FALSE) + + ## and can be exported as BED + methSeg2bed(segments = segRaw, filename = "output_seg_raw.bed") + methSeg2bed(segments = segDiff, filename = "output_seg_diff.bed") + #end if + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + input_type['choice'] in ['all', 'differential_methylation'] + + + + input_type['choice'] in ['all', 'differential_methylation'] + + + + input_type['choice'] in ['all', 'differential_methylation'] + + + + input_type['choice'] in ['all', 'clustering'] + + + + input_type['choice'] in ['all', 'clustering'] + + + + input_type['choice'] in ['all', 'segmentation'] + + + + input_type['choice'] in ['all', 'segmentation'] + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 10.1186/gb-2012-13-10-r87 + + diff -r 000000000000 -r a8705df7c57f test-data/input_control1.myCpG.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/input_control1.myCpG.txt Wed Dec 21 17:30:57 2016 -0500 @@ -0,0 +1,2378 @@ +chrBase chr base strand coverage freqC freqT +chr21.9826907 chr21 9826907 F 96 18.75 81.25 +chr21.9853326 chr21 9853326 F 16 87.50 12.50 +chr21.9853296 chr21 9853296 F 18 88.89 11.11 +chr21.9860126 chr21 9860126 F 83 100.00 0.00 +chr21.9906663 chr21 9906663 R 14 92.86 7.14 +chr21.9906677 chr21 9906677 R 14 78.57 21.43 +chr21.9906700 chr21 9906700 F 30 26.67 73.33 +chr21.9906954 chr21 9906954 F 30 86.67 13.33 +chr21.9906644 chr21 9906644 F 23 95.65 4.35 +chr21.9906873 chr21 9906873 F 15 26.67 73.33 +chr21.9906634 chr21 9906634 F 23 69.57 30.43 +chr21.9906704 chr21 9906704 F 18 94.44 5.56 +chr21.9906655 chr21 9906655 R 14 92.86 7.14 +chr21.9906645 chr21 9906645 R 13 100.00 0.00 +chr21.9906926 chr21 9906926 R 17 64.71 35.29 +chr21.9906619 chr21 9906619 F 22 81.82 18.18 +chr21.9906949 chr21 9906949 F 30 53.33 46.67 +chr21.9906910 chr21 9906910 R 16 56.25 43.75 +chr21.9906907 chr21 9906907 R 17 11.76 88.24 +chr21.9906681 chr21 9906681 F 37 37.84 62.16 +chr21.9906955 chr21 9906955 R 13 69.23 30.77 +chr21.9906724 chr21 9906724 F 18 72.22 27.78 +chr21.9906822 chr21 9906822 R 14 100.00 0.00 +chr21.9906808 chr21 9906808 R 13 100.00 0.00 +chr21.9906847 chr21 9906847 R 14 71.43 28.57 +chr21.9906660 chr21 9906660 R 14 64.29 35.71 +chr21.9906839 chr21 9906839 R 13 69.23 30.77 +chr21.9906846 chr21 9906846 F 15 93.33 6.67 +chr21.9906928 chr21 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01 00:00:00 1970 +0000 +++ b/test-data/output_seg_diff.bed Wed Dec 21 17:30:57 2016 -0500 @@ -0,0 +1,4 @@ +track name="meth segments" description="meth segments" itemRgb=on +chr21 9853000 13991000 1 -10.9674674784423 . 9853000 13991000 190,190,190 +chr21 13991000 14019000 2 17.5358121475575 . 13991000 14019000 0,100,0 +chr21 14057000 19944000 1 -7.21990925670131 . 14057000 19944000 140,207,140 diff -r 000000000000 -r a8705df7c57f test-data/output_seg_raw.bed --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output_seg_raw.bed Wed Dec 21 17:30:57 2016 -0500 @@ -0,0 +1,6 @@ +track name="meth segments" description="meth segments" itemRgb=on +chr21 9764000 14382000 2 68.3360848593166 . 9764000 14382000 0,227,0 +chr21 14510000 14774000 1 37.2301737330093 . 14510000 14774000 162,199,162 +chr21 14791000 15242000 3 93.231961836613 . 14791000 15242000 0,100,0 +chr21 15286000 15583000 1 32.8700430825969 . 15286000 15583000 190,190,190 +chr21 15583000 19944000 2 67.8414022933781 . 15583000 19944000 0,230,0 diff -r 000000000000 -r a8705df7c57f test-data/output_statistics.pdf Binary file test-data/output_statistics.pdf has changed diff -r 000000000000 -r a8705df7c57f test-data/test.r --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/test.r Wed Dec 21 17:30:57 2016 -0500 @@ -0,0 +1,60 @@ +library("methylKit") + +file.list = list("input_test1.myCpG.txt", "input_test2.myCpG.txt", "input_control1.myCpG.txt", "input_control2.myCpG.txt") + +myobj=methRead( file.list, + sample.id=list("test 1","test 2","control 1","control 2"),assembly="hg18",pipeline="amp",treatment=c(1,1,0,0)) + +pdf('output_statistics.pdf') +for (obj in myobj){ + getMethylationStats(obj,plot=TRUE,both.strands=FALSE) + getCoverageStats(obj,plot=TRUE,both.strands=FALSE) +} +devname = dev.off() + +# unite function +methidh = unite(myobj) + +pdf("output_correlation.pdf") +getCorrelation(object = methidh, plot=TRUE, method = "pearson") +devname = dev.off() + +# differential methylation +myDiff = calculateDiffMeth(methidh, overdispersion="none", + adjust="SLIM", effect="wmean", test="Chisq", + slim=FALSE, weighted.mean=FALSE) + +bedgraph(myDiff, file.name="output_myDiff.bedgraph", col.name="meth.diff", + unmeth=FALSE, log.transform=FALSE, negative=FALSE, add.on="") + +MethPerChr = diffMethPerChr(myDiff, plot=FALSE, + qvalue.cutoff=0.01, + meth.cutoff=25) +write.table(MethPerChr, sep="\t", row.names=FALSE, quote=FALSE, file="output_MethPerChr.tsv") + +MethylDiff = getMethylDiff(myDiff, difference=25, + qvalue=0.01, type="all") +bedgraph(MethylDiff, file.name="output_MethylDiff.bedgraph", col.name="meth.diff", + unmeth=FALSE,log.transform=FALSE,negative=FALSE,add.on="") + +pdf( "output_clustering.pdf" ) +methClust = clusterSamples(methidh, dist="correlation", method="ward") +devname = dev.off() + +pdf( "output_PCA.pdf" ) +PCASamples(methidh) +devname = dev.off() + +## methSeg works for methylRaw or methylDiff with resolution region, +## so methylBase has to be tiled before +tileRaw = tileMethylCounts(myobj[[1]]) +tileBase = tileMethylCounts(methidh) +tileDiff = calculateDiffMeth(tileBase) + +## methseg generates Granges +segRaw = methSeg(tileRaw, diagnostic.plot = FALSE) +segDiff = methSeg(tileDiff, diagnostic.plot = FALSE) + +## and can be exported as BED +methSeg2bed(segments = segRaw, filename = "output_seg_raw.bed") +methSeg2bed(segments = segDiff, filename = "output_seg_diff.bed")