Mercurial > repos > rnateam > mirdeep2_mapper
changeset 4:dbbe92348c7a draft default tip
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/mirdeep2/mirdeep2_mapper commit 04c75332ac618b43ce5c3f307f7866e97147e865
| author | rnateam |
|---|---|
| date | Thu, 05 Apr 2018 08:55:27 -0400 |
| parents | a8d24f4b6d95 |
| children | |
| files | mapper.xml test-data/reads_sample1.fa test-data/reads_sample2.fa |
| diffstat | 3 files changed, 1096 insertions(+), 33 deletions(-) [+] |
line wrap: on
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--- a/mapper.xml Wed Jul 12 14:38:46 2017 -0400 +++ b/mapper.xml Thu Apr 05 08:55:27 2018 -0400 @@ -1,4 +1,4 @@ -<tool id="rbc_mirdeep2_mapper" name="MiRDeep2 Mapper" version="2.0.0"> +<tool id="rbc_mirdeep2_mapper" name="MiRDeep2 Mapper" version="2.0.0.8.1"> <description>process and map reads to a reference genome</description> <macros> <macro name="map_params"> @@ -28,36 +28,38 @@ <requirements> <requirement type="package" version="2.0.0.8">mirdeep2</requirement> </requirements> - <stdio> - <!-- Anything other than zero is an error --> - <exit_code range="1:" /> - <exit_code range=":-1" /> - <!-- In case the return code has not been set propery check stderr too --> - <regex match="Error:" /> - <regex match="Exception:" /> - </stdio> - <command> + <command detect_errors="aggressive"> <![CDATA[ - #if $operation.collapse_map == "collapse_and_map" or $operation.collapse_map == "only_map" #if $operation.refGenomeSource.genomeSource == "history" bowtie-build '$operation.refGenomeSource.ownFile' custom_bowtie_indices && #end if #end if - mapper.pl - - '$reads' - - #if $reads.extension.startswith("fasta") - -c - #else if $reads.extension.startswith("fastq") - -e - -h + mapper.pl + + #if $input.type == "single": + '$input.reads' + + #if $input.reads.extension.startswith("fasta") + -c + #else if $input.reads.extension.startswith("fastq") + -e + -h + #end if + #else: + '$samples' -d + + #if $input.reads_list[0].reads.extension.startswith("fasta") + -c + #else if $input.reads_list[0].reads.extension.startswith("fastq") + -e + -h + #end if #end if $remove_non_canon - + $convert_rna_dna #if $clip_adapter.clip == "true" @@ -69,10 +71,10 @@ #if $operation.collapse_map == "collapse_and_map" or $operation.collapse_map == "only_collapse" -m -s '$output_reads_collapsed' #end if - + #if $operation.collapse_map == "collapse_and_map" or $operation.collapse_map == "only_map" - -p - + -p + #if $operation.refGenomeSource.genomeSource == "history" custom_bowtie_indices #else @@ -80,15 +82,38 @@ #end if $operation.map_mismatch -r $operation.map_threshold - + -t '$output_mapping' #end if -v -n ]]> </command> + <configfiles> + <configfile name="samples"><![CDATA[#if $input.type == "multiple": +#for $r in $input.reads_list: +$r.reads $r.sample_name +#end for +#end if]]></configfile> + </configfiles> <inputs> - <param format="fastq, fasta" name="reads" type="data" optional="false" label="Deep sequencing reads" help="Reads in fastq or FASTA format"/> + <conditional name="input"> + <param name="type" type="select" label="Pool multiple read sets"> + <option value="single" selected="true">No</option> + <option value="multiple">Yes</option> + </param> + <when value="single"> + <param format="fastq,fasta" name="reads" type="data" label="Deep sequencing reads" help="Reads in fastq or FASTA format"/> + </when> + <when value="multiple"> + <repeat name="reads_list" title="Reads"> + <param name="sample_name" value="" type="text" label="Sample name" help="Must be a 3 letters/digits code"> + <validator type="expression" message="The sample name must be a 3 letters/digits code">len(value) == 3 and value.isalnum()</validator> + </param> + <param format="fastq,fasta" name="reads" type="data" optional="false" label="Deep sequencing reads" help="Reads in fastq or FASTA format"/> + </repeat> + </when> + </conditional> <param name="remove_non_canon" type="boolean" truevalue="-j" falsevalue="" checked="false" label="Remove reads with non-standard nucleotides" help="Remove all entries that have a sequence that contains letters other than a,c,g,t,u,n,A,C,G,T,U,N. (-j)"/> <param name="convert_rna_dna" type="boolean" truevalue="-i" falsevalue="" checked="false" label="Convert RNA to DNA alphabet (to map against genome)" help="(-i)"/> @@ -104,11 +129,11 @@ </when> <when value="false"/> </conditional> - + <param name="discard_short_reads" value="18" type="integer" optional="false" label="Discard reads shorter than this length" help="Set to 0 to keep all reads. (-l)"> <validator type="in_range" min="0" message="Minimum value is 0"/> </param> - + <conditional name="operation"> <param name="collapse_map" type="select" label="Collapse reads and/or Map" help="(-m) and/or (-p)"> <option value="collapse_and_map">Collapse reads and Map</option> @@ -144,7 +169,10 @@ </outputs> <tests> <test> - <param name="reads" value="reads.fa"/> + <conditional name="input"> + <param name="type" value="single"/> + <param name="reads" value="reads.fa"/> + </conditional> <param name="remove_non_canon" value="True"/> <param name="clip" value="true"/> <param name="adapter_seq" value="TCGTATGCCGTCTTCTGCTTGT"/> @@ -171,22 +199,57 @@ </assert_contents> </output> </test> + <test> + <conditional name="input"> + <param name="type" value="multiple"/> + <repeat name="reads_list"> + <param name="sample_name" value="sa1"/> + <param name="reads" value="reads_sample1.fa"/> + </repeat> + <repeat name="reads_list"> + <param name="sample_name" value="sa2"/> + <param name="reads" value="reads_sample2.fa"/> + </repeat> + </conditional> + <param name="remove_non_canon" value="True"/> + <param name="clip" value="true"/> + <param name="adapter_seq" value="TCGTATGCCGTCTTCTGCTTGT"/> + <param name="discard_short_reads" value="18"/> + <param name="collapse_map" value="collapse_and_map"/> + <param name="genomeSource" value="history"/> + <param name="ownFile" value="cel_cluster.fa"/> + <output name="output_reads_collapsed"> + <assert_contents> + <has_text text=">sa1_220_x1"/> + <has_text text="TCACCGGGTGTACATCAGC"/> + <has_text text=">sa2_0_x250"/> + <has_text text="AATGACACTGGTTATCTTTTCCATCG"/> + </assert_contents> + </output> + <output name="output_mapping"> + <assert_contents> + <has_line_matching expression="^.*22\t1\t22\ttcaccgggtggaaactagcagt\tchrII:11534525-11540624\t22\t3060\t3081.*$"/> + <has_line_matching expression="^.*21\t1\t21\ttcaccgggtggaaactagcag\tchrII:11534525-11540624\t21\t3060\t3080.*$"/> + <has_line_matching expression="^.*22\t1\t22\ttcaccgggtgtacatcagctaa\tchrII:11534525-11540624\t22\t3631\t3652.*$"/> + </assert_contents> + </output> + </test> </tests> <help> <![CDATA[ **What it does** -The MiRDeep2 Mapper module is designed as a tool to process deep sequencing reads and/or map them to the reference genome. -The module works in sequence space, and can process or map data that is in sequence FASTA format. +The MiRDeep2 Mapper module is designed as a tool to process deep sequencing reads and/or map them to the reference genome. +The module works in sequence space, and can process or map data that is in sequence FASTA format. A number of the functions of the mapper module are implemented specifically with Solexa/Illumina data in mind. **Input** -Default input is a file in FASTA format, seq.txt or qseq.txt format. More input can be given depending on the options used. +Default input is a file in FASTA format, seq.txt or qseq.txt format. More input can be given depending on the options used. **Output** -The output depends on the options used. Either a FASTA file with processed reads or an arf file with with mapped reads, or both, are output. +The output depends on the options used. Either a FASTA file with processed reads or an arf file with with mapped reads, or both, are output. Arf format: Is a proprietary file format generated and processed by miRDeep2. It contains information of reads mapped to a reference genome. Each line in such a file contains 13 columns:
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/reads_sample1.fa Thu Apr 05 08:55:27 2018 -0400 @@ -0,0 +1,500 @@ +>nematode_1 +TCACCGGGTGTAAATCAGCTTGTCGTATGCCGTCT +>nematode_2 +TCACCGGGTGAACACTTGCAGTTCGTATGACGTCT +>nematode_3 +TCACCGGGTGAACACTTGCAGTTCGTATGCCGTCT +>nematode_4 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_5 +TCACCGGGTGGAAACTAGCTGTCGTATGCCGTCTT +>nematode_6 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_7 +TCACCGGGGGGAAACTAGCAGTTCGTATGCTGTTT +>nematode_8 +TCACCGGGTGAACACCTGCAGTTCGTATGCCGTCT +>nematode_9 +TCACCGGGCGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_10 +TCACCGGGTGAACACTTGCAGTTCGTATGCCGTCT +>nematode_11 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_12 +TCACCGGGTGAACACTTGCAGTTCGTATGCCGTCT +>nematode_13 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_14 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_15 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_16 +TCACCGGGTGAACACTTGCAGTTCGTATGCCGTCT +>nematode_17 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_18 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+TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_190 +TCACCGGGGGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_191 +TCACCGGGTGAACACTTGCAGTTCGTATGCCTTCT +>nematode_192 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_193 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_194 +TCACCGGGTGGTAACTAGCAGTTCGTATGCCGTCT +>nematode_195 +TCACCGGGTGGAAACTAGCAGTTCGTATGACGCCT +>nematode_196 +TCACCGGGAGAACACTTGCAGTTCGTATGCTGTCT +>nematode_197 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_198 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_199 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_200 +TCACCGGGTGAAAATTCGCATGTCGTATGCCGTCT +>nematode_201 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_202 +TCACCGGGTGAACACTTGCAGTTCGTATGCCGTCT +>nematode_203 +TCACCGGGCGAACACTTGCAGTTCGTATGACGTCT +>nematode_204 +TCACAGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_205 +TCACCGGGTGAAAATTCGCATGTCGTATGCCGTCT +>nematode_206 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_207 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_208 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_209 +TCACCGGGCGAACACTTGCAGTTCGTATGCCGTCT +>nematode_210 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_211 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_212 +TCACCGGGTGAACACTTGCAGTTCGTATGCCGTCT +>nematode_213 +TCACCGGGCGGAAACTAGCAGTTCGTATCCCTTCT +>nematode_214 +TCACCGGGTGTACTTCAGCTAATCGTATGCCGTCT +>nematode_215 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_216 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_217 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_218 +TCACCGGGTGAACACTTGCAGTTCGTATGCCGTCT +>nematode_219 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_220 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_221 +TCACCGGGTGGAAACTAGCAGTCGTATGCCGTCTT +>nematode_222 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_223 +TCACCGGGTGAAAATTCGCATGTCGTATGCCGTCT +>nematode_224 +TCACCGGGAGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_225 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_226 +TCACCGGGTGGAAACTAGCAGTTCGTATGCTGTCT +>nematode_227 +TCACCGGGTGAACACTTGCAGTTCGTATGCCGTCT +>nematode_228 +TCACCGGGGGAACACTTGCAGTTCGTATGCCGTCT +>nematode_229 +TCACCGGGTGGAAACTAGCAGTTTCGTATGCCGTC +>nematode_230 +TCACAGGGTGTACATCAGCTAATCGTATGCCGTCT +>nematode_231 +TCACCGGGTGAACACTTGCAGTTCGTATGCCGTCT +>nematode_232 +TCACCGGGTGAACACTTGCAGTTCGTATGCCGTCT +>nematode_233 +TCACCGGGTGAAAATTCGCATGTCGTATGCCGTCT +>nematode_234 +TCACCGGGTGAACACTTGCAGTTCGTATGCAGTCT +>nematode_235 +TCACCGGGTGGAAACTAGCAGTCGTATGCCGTCTT +>nematode_236 +TCACCGGGTGTACATCAGCTCGTATGCCGTCTTCT +>nematode_237 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_238 +TCACCGGGTGAACACTTGCAGTTCGTATGCCGTCT +>nematode_239 +TCACCGGGTGGAAACTAGCAGTTTCGTATGCCGTC +>nematode_240 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_241 +TCACCGGGTGGAAACTAGCAGTCGTATGCCGTCTT +>nematode_242 +TCACCGGGTGTACATCAGCTAATTCGTATGCCGTC +>nematode_243 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_244 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_245 +TCACCGGGTGAACACTTGCAGTTCGTATGCCGTCT +>nematode_246 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT +>nematode_247 +TCACCGGGTGAACACTTGCAGTTCGTATGCCGTCT +>nematode_248 +TCACCGGGTGAAAATTCGCATGTCGTATGCCGTCT +>nematode_249 +TCACCGGGTGAACACTTGCAGTTCGTATGCCGTCT +>nematode_250 +TCACCGGGTGGAAACTAGCAGTTCGTATGCCGTCT
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/reads_sample2.fa Thu Apr 05 08:55:27 2018 -0400 @@ -0,0 +1,500 @@ +>nematode_378083 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378084 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378085 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378086 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378087 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378088 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378089 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378090 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378091 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378092 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378093 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378094 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378095 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378096 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378097 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378098 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378099 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378100 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378101 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378102 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378103 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378104 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378105 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378106 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378107 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378108 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378109 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378110 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378111 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378112 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378113 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378114 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378115 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378116 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378117 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378118 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378119 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378120 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378121 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378122 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378123 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378124 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378125 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378126 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378127 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378128 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378129 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378130 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378131 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378132 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378133 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378134 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378135 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378136 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378137 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378138 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378139 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378140 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378141 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378142 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378143 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378144 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378145 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378146 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378147 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378148 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378149 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378150 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378151 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378152 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378153 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378154 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378155 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378156 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378157 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378158 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378159 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378160 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378161 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378162 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378163 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378164 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378165 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378166 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378167 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378168 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378169 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378170 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378171 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378172 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378173 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378174 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378175 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378176 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378177 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378178 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378179 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378180 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378181 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378182 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378183 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378184 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378185 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378186 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378187 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378188 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378189 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378190 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378191 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378192 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378193 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378194 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378195 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378196 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378197 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378198 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378199 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378200 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378201 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378202 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378203 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378204 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378205 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378206 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378207 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378208 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378209 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378210 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378211 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378212 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378213 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378214 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378215 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378216 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378217 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378218 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378219 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378220 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378221 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378222 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378223 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378224 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378225 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378226 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378227 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378228 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378229 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378230 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378231 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378232 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378233 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378234 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378235 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378236 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378237 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378238 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378239 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378240 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378241 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378242 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378243 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378244 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378245 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378246 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378247 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378248 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378249 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378250 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378251 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378252 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378253 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378254 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378255 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378256 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378257 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378258 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378259 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378260 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378261 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378262 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378263 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378264 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378265 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378266 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378267 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378268 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378269 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378270 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378271 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378272 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378273 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378274 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378275 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378276 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378277 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378278 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378279 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378280 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378281 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378282 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378283 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378284 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378285 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378286 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378287 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378288 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378289 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378290 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378291 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378292 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378293 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378294 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378295 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378296 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378297 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378298 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378299 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378300 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378301 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378302 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378303 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378304 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378305 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378306 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378307 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378308 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378309 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378310 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378311 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378312 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378313 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378314 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378315 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378316 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378317 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378318 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378319 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378320 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378321 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378322 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378323 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378324 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378325 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378326 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378327 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378328 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378329 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378330 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378331 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC +>nematode_378332 +AATGACACTGGTTATCTTTTCCATCGTCGTATGC