Mercurial > repos > romaingred > pirna_pipeline
changeset 11:ee6b4b2072a9 draft
Uploaded
author | romaingred |
---|---|
date | Fri, 13 Oct 2017 08:46:31 -0400 |
parents | 42bc59c7db3a |
children | eec6ce306605 |
files | bin/Rcall.pm |
diffstat | 1 files changed, 39 insertions(+), 4 deletions(-) [+] |
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--- a/bin/Rcall.pm Fri Oct 13 04:20:17 2017 -0400 +++ b/bin/Rcall.pm Fri Oct 13 08:46:31 2017 -0400 @@ -49,9 +49,17 @@ $R->send( qq`library('Sushi') fai =read.table("$fai") - bgP = read.table("$bgP") - bgM = read.table("$bgM") - fun = function(chr,end) { + if ( file.info("$bgP")\$size !=0 ) + { + bgP = read.table("$bgP") + } else { bgP = data.frame(factor(),integer()) } + + if ( file.info("$bgM")\$size !=0 ) + { + bgM = read.table("$bgM") + } else { bgM = data.frame(factor(),integer()) } + + f_both = function(chr,end) { jpeg( paste0("$dir",as.character(chr),".png"), quality=100) par(mfrow=c(2,1),mar=c(1,10,1,3)) plotBedgraph(bgP, chrom=chr,chromstart=0,chromend=end,transparency=.50, color=SushiColors(2)(2)[1]) @@ -63,7 +71,34 @@ mtext("Scaled Read Depth",side=2,line=4.5,cex=1,font=2) dev.off() } - mapply( fun, fai\$V1, fai\$V2)`); + + f_plus = function(chr,end) { + jpeg( paste0("$dir",as.character(chr),".png"), quality=100) + plotBedgraph(bgP, chrom=chr,chromstart=0,chromend=end,transparency=.50, color=SushiColors(2)(2)[1]) + labelgenome(chrom=chr,chromstart=0,chromend=end,n=3,scale="$sb", line=0, chromline = 0.5, scaleline = 0.5, scaleadjust =1.05, chromadjust = -0.4) + axis(side=2,las=2,tcl=.2) + mtext("Scaled Read Depth",side=2,line=4,cex=1,font=2) + dev.off() + } + + f_minus = function(chr,end) { + jpeg( paste0("$dir",as.character(chr),".png"), quality=100) + plotBedgraph(bgM, chrom=chr,chromstart=0,chromend=end,transparency=.50, flip=TRUE, color=SushiColors(2)(2)[2]) + labelgenome(chrom=chr,chromstart=0,chromend=end,n=3,scale="$sb", line=0, chromline = 0.5, scaleline = 0.5, scaleadjust =1.05, chromadjust = -0.4) + axis(side=2,las=2,tcl=.2,at=pretty(par("yaxp")[c(1,2)]),labels=-1*pretty(par("yaxp")[c(1,2)])) + mtext("Scaled Read Depth",side=2,line=4.5,cex=1,font=2) + dev.off() + } + + fai_b = fai[fai\$V1 %in% intersect(bgM\$V1,bgP\$V1), ] + mapply( f_both, fai_b\$V1, fai_b\$V2) + + fai_p = fai[fai\$V1 %in% setdiff(bgP\$V1,bgM\$V1), ] + mapply( f_plus, fai_p\$V1, fai_p\$V2) + + fai_m = fai[fai\$V1 %in% setdiff(bgM\$V1,bgP\$V1), ] + mapply( f_minus, fai_m\$V1, fai_m\$V2) `); + $R->stopR(); }