Mercurial > repos > sarahinraauzeville > star
view README_STARV2 @ 3:aeebcdb9b8b2 draft
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author | sarahinraauzeville |
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date | Tue, 12 Dec 2017 10:16:00 -0500 |
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************************************************************************************ ****** Add perl and xml wrapper files in tools/ d ****** ************************************************************************************ Download files with hg clone in tools/ directory, then you have these files availables (wrappers and scripts): sm_STAR2_V2.pl sm_STAR2_V2.xml GalaxyPath.pm STAR_indexes.loc.sample STAR_indexes.loc.sample Add GalaxyPath.pm file in /path/to/galaxy_sources/ directory ************************************************************************************ ****** loc file ****** ************************************************************************************ mv STAR_indexes.loc.sample STAR_indexes.loc then Add STAR_indexes.loc in yout tool-data/ repository ************************************************************************************ ****** tool_conf.xml file to update ****** ************************************************************************************ Add a tag <tool> in your section "RNAseq" (for instance) in your config/tool_conf.xml file: <tool file="/path/to/sm_STAR2_V2.xml" /> ************************************************************************************ ****** job_conf.xml file to update ****** ************************************************************************************ Add a new section in config/job_conf.xml file, in <destinations> tag, add these lines: <destination id="sm_star_single_V2_job" runner="drmaa"> <param id="galaxy_external_runjob_script">scripts/drmaa_external_runner.py</param> <param id="galaxy_external_killjob_script">scripts/drmaa_external_killer.py</param> <param id="galaxy_external_chown_script">scripts/external_chown_script.py</param> <param id="nativeSpecification">-clear -V -q galaxy.q -l mem=12G -l h_vmem=16G -pe parallel_smp 2</param> <env file="/galaxydata/galaxy-prod/my_config/local_env.sh"/> </destination> and in <tools> tag: <tool id="sm_star_single_V2" destination="sm_star_single_V2_job"/> ************************************************************************************ ****** tool_data_table_conf.xml file to update ****** ************************************************************************************ Add these lines in your tool_data_table_conf.xml file: <!-- location of loc file for STAR V2 tool --> <table name="STAR_indexes" comment_char="#"> <columns>value, dbkey, name, path</columns> <file path="my_tool-data/STAR_indexes.loc" /> </table> ************************************************************************************ ****** Add paths in galaxy.ini configuation file ****** ************************************************************************************ Add a my_bin/ directory in galaxy repository with symbolic links to binaries: $ ls -ltrah /path/to/galaxy/my_bin/STAR* lrwxrwxrwx 1 galaxy-prod wbioinfo 11 3 juin 2016 /galaxydata/galaxy-prod/my_bin/STAR -> STAR_2.4.0i Add paths to binaries and a section [workPath], at the end of galaxy.ini file: Dans geany ../../config/galaxy.ini & [workPath] STAR_PATH=/path/to/galaxy/my_bin/STAR Add a my_workspace/ directory in galaxy repository to run this module in debug mode: cd /path/to/galaxy/; mkdir my_workspace/; chmod 777 my_workspace/; Add paths to binaries and a section [workPath], at the end of galaxy.ini file: Dans geany ../../config/galaxy.ini & [workPath] MYWORKSPACE=/path/to/galaxy/my_workspace/