diff pyPRADA_1.2/tools/samtools-0.1.16/misc/zoom2sam.pl @ 0:acc2ca1a3ba4

Uploaded

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/pyPRADA_1.2/tools/samtools-0.1.16/misc/zoom2sam.pl	Thu Feb 20 00:44:58 2014 -0500
@@ -0,0 +1,97 @@
+#!/usr/bin/perl -w
+
+# Contact: lh3
+# Version: 0.1.0
+
+use strict;
+use warnings;
+use Getopt::Std;
+
+&zoom2sam;
+exit;
+
+sub mating {
+  my ($s1, $s2) = @_;
+  my $isize = 0;
+  if ($s1->[2] ne '*' && $s1->[2] eq $s2->[2]) { # then calculate $isize
+	my $x1 = ($s1->[1] & 0x10)? $s1->[3] + length($s1->[9]) : $s1->[3];
+	my $x2 = ($s2->[1] & 0x10)? $s2->[3] + length($s2->[9]) : $s2->[3];
+	$isize = $x2 - $x1;
+  }
+  # update mate coordinate
+  if ($s2->[2] ne '*') {
+	@$s1[6..8] = (($s2->[2] eq $s1->[2])? "=" : $s2->[2], $s2->[3], $isize);
+	$s1->[1] |= 0x20 if ($s2->[1] & 0x10);
+  } else {
+	$s1->[1] |= 0x8;
+  }
+  if ($s1->[2] ne '*') {
+	@$s2[6..8] = (($s1->[2] eq $s2->[2])? "=" : $s1->[2], $s1->[3], -$isize);
+	$s2->[1] |= 0x20 if ($s1->[1] & 0x10);
+  } else {
+	$s2->[1] |= 0x8;
+  }
+}
+
+sub zoom2sam {
+  my %opts = ();
+  getopts("p", \%opts);
+  die("Usage: zoom2sam.pl [-p] <readLen> <aln.zoom>
+Warnings: This script only supports the default Illumina outputs.\n") if (@ARGV < 2);
+  my $is_paired = defined($opts{p});
+  my $len = shift(@ARGV);
+  # core loop
+  my @s1 = ();
+  my @s2 = ();
+  my ($s_last, $s_curr) = (\@s1, \@s2);
+  while (<>) {
+	&zoom2sam_aux($_, $s_curr, $is_paired, $len);
+	if (@$s_last != 0 && $s_last->[0] eq $s_curr->[0]) {
+	  &mating($s_last, $s_curr);
+	  print join("\t", @$s_last), "\n";
+	  print join("\t", @$s_curr), "\n";
+	  @$s_last = (); @$s_curr = ();
+	} else {
+	  print join("\t", @$s_last), "\n" if (@$s_last != 0);
+	  my $s = $s_last; $s_last = $s_curr; $s_curr = $s;
+	}
+  }
+  print join("\t", @$s_last), "\n" if (@$s_last != 0);
+}
+
+sub zoom2sam_aux {
+  my ($line, $s, $is_paired, $len) = @_;
+  chomp($line);
+  my @t = split("\t", $line);
+  @$s = ();
+  # read name
+  $s->[0] = $t[0];
+  # initial flag (will be updated later)
+  $s->[1] = 0;
+  $s->[1] |= 1 | 1<<6 if ($s->[0] =~ /_F$/);
+  $s->[1] |= 1 | 1<<7 if ($s->[0] =~ /_R$/);
+  $s->[1] |= 2 if ($is_paired);
+  # read & quality
+  $s->[9] = "*"; $s->[10] = "*";
+  # cigar
+  $s->[5] = $len . "M";
+  # coor
+  my @s = split(/\s+/, $t[1]);
+  $s->[2] = $s[0];
+  $t[1] =~ /:(\d+)$/;
+  $s->[3] = $1 + 1;
+  if ($s->[0] =~ /_[FR]$/) {
+	my $u = ($s->[0] =~ /_F$/)? 1 : 0;
+	my $w = ($t[2] eq '+')? 1 : 0;
+	$s->[1] |= 0x10 if ($u ^ $w);
+	$s->[0] =~ s/_[FR]$//;
+  } else {
+	$s->[1] |= 0x10 if ($t[2] eq '-');
+  }
+  # mapQ
+  $s->[4] = 30;
+  # mate coordinate
+  $s->[6] = '*'; $s->[7] = $s->[8] = 0;
+  # aux
+  push(@$s, "NM:i:$t[3]");
+}