Mercurial > repos > spanish_national_institue_of_bioinformatics > nucleosome_dynamics
diff nucleR_stats.xml @ 2:e88c806ddf3e draft default tip
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author | spanish_national_institue_of_bioinformatics |
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date | Fri, 12 Apr 2019 05:28:43 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/nucleR_stats.xml Fri Apr 12 05:28:43 2019 -0400 @@ -0,0 +1,73 @@ +<tool id="nucleR_stats" name="NucleR Statistics" version="0.1"> + <description>: gather gene and genome-wide statistics on NucleR results</description> + <requirements> + <requirement type="binary">docker</requirement> + </requirements> + <command> + <![CDATA[ + docker run -v $__root_dir__/database/files:$__root_dir__/database/files -v /data:/data -v /tmp:/tmp -u `id -u`:`id -g` mmbirb/nucleosome-dynamics nucleR_stats --input $gff_file --out_genes $output_genes_csv_file --out_gw $output_gw_csv_file + #if $assembly.source == "buildin": + --genome ${assembly.ref_genome_gff_buildin} + #else if $assembly.source == "history": + --genome ${assembly.ref_genome_gff_history} + #end if; + ]]> + </command> + <inputs> + <param name="gff_file" type="data" format="gff" label="Nucleosome Calls (GFF)" help="Nucleosome Calls in GFF format as obtained from nucleR" /> + <conditional name="assembly"> + <param name="source" type="select" label="Select a built-in reference genome or use one from your history" help="Taking from each assembly their annotated genes."> + <option value="buildin" selected="True">Use a built-in genome</option> + <option value="history">Use a genome from the history</option> + </param> + <when value="buildin"> + <param name="ref_genome_gff_buildin" type="select" label="Select reference genome (Genes)" help="Select gene annotations for your reference genome. If your genome of interest is not listed, contact the Galaxy team."> + <options from_file="nucldyn_publicdata.loc"> + <column name="name" index="2"/> + <column name="value" index="4"/> + </options> + </param> + </when> + <when value="history"> + <param name="ref_genome_gff_history" type="data" format="gff" label="Reference genome (Genes)" help="Upload gene annotations for your reference genome. Check below the documentation for learning about the file format."/> + </when> + </conditional> +</inputs> + <outputs> + <data format="csv" name="output_genes_csv_file" label="NR__stats__${os.path.splitext(($gff_file.name.split('__'))[1])[0]}.genes.csv" /> + <data format="csv" name="output_gw_csv_file" label="NR__stats__${os.path.splitext(($gff_file.name.split('__'))[1])[0]}.gw.csv" /> + </outputs> + <tests> + <test> + <param name="gff_file" value="NR__cellcycleM_chrII.gff" /> + <param name="ref_genome_gff_buildin" value="/data/nucldyn_publicdata/refGenomes/R64-1-1/genes.gff" /> + <output name="output_genes_csv_file" value="NR__stats__cellcycleM_chrII.genes.csv" /> + <output name="output_gw_csv_file" value="NR__stats__cellcycleM_chrII.gw.csv"/> + </test> + </tests> + <help> +.. image:: ${static_path}/images/NucleosomeDynamicsLogo.png + :height: 80 + :width: 200 + +----- + +Nucleosome Dynamics is a set of tools that take MNase-seq and ATAC-seq aligned reads and performs a serie of nucleosome-related analyses on them. + +.. class:: infomark + +Visit the documentation of the original application for learning more about the accepted values and formats. http://mmb.irbbarcelona.org/NucleosomeDynamics/help/usage/nucleosome-dynamics + + </help> + <citations> + <citation type="bibtex"> +@misc{github, + author = {Buitrago D}, + year = {2019}, + title = {Nucleosome Dynamics suite: containerized installation}, + publisher = {GitHub}, + journal = {GitHub repository}, + url = {https://github.com/nucleosome-dynamics/nucleosome_dynamics}, +}</citation> + </citations> +</tool>