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1 <tool id="idba_ud" name="idba_ud" version="1.1.1 (Oct 18, 2012)">
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2 <description>IDBA-UD - Iterative de Bruijn Graph Assembler for sequencing data with highly uneven depth.</description>
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3 <command>
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4
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5 idba_ud
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6
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7 --read $read
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8
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9 #if $long_read:
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10
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11 --long_read $long_read
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12
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13 #end if
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14
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15 #if $read_level_2:
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16
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17 --read_level_2 $read_level_2
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18
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19 #end if
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20
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21 #if $read_level_3:
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22
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23 --read_level_3 $read_level_3
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24
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25 #end if
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26
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27 #if $read_level_4:
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28
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29 --read_level_4 $read_level_4
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30
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31 #end if
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32
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33 #if $read_level_5:
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34
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35 --read_level_5 $read_level_5
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36
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37 #end if
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38
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39 --mink $mink --maxk $maxk $step --inner_mink $inner_mink --inner_step $inner_step --prefix $prefix --min_count $min_count --min_support $min_support --num_threads $num_threads --seed_kmer $seed_kmer --min_contig $min_contig --similar $similar --max_mismatch $max_mismatch --min_pairs $min_pairs
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40
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41 #if str( $other ) != "None":
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42
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43 $other
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44
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45 #end if
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46
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47 ;
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48
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49 mv out/scaffold.fa $output
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50
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51 </command>
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52
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53 <inputs>
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54 <param name="read" type="data" format="fasta" label="Fasta read file. Lower or equal to 250b (--read)"/>
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55 <param name="long_read" type="data" format="fasta" optional="true" label="Fasta long read file. More than 250b (--long_read)"/>
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56
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57 <param name="read_level_2" type="data" format="fasta" optional="true" label="Fasta Paired-end reads for second level scaffolds (--read_level_2)"/>
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58 <param name="read_level_3" type="data" format="fasta" optional="true" label="Fasta Paired-end reads for third level scaffolds (--read_level_3)"/>
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59 <param name="read_level_4" type="data" format="fasta" optional="true" label="Fasta Paired-end reads for fourth level scaffolds (--read_level_4)"/>
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60 <param name="read_level_5" type="data" format="fasta" optional="true" label="Fasta Paired-end reads for fifth level scaffolds (--read_level_5)"/>
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61
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62 <param name="mink" type="integer" value="20" max="124" label="Minimum k value. Lower or equal to 124 (--mink)"/>
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63 <param name="maxk" type="integer" value="100" max="124" label="Maximum k value. Lower or equal to 124 (--maxk)"/>
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64 <param name="step" type="integer" value="20" label="Increment of k-mer of each iteration (--step)"/>
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65 <param name="inner_mink" type="integer" value="10" label="Inner minimum k value (--inner_mink)"/>
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66 <param name="inner_step" type="integer" value="5" label="Inner increment of k-mer (--inner_step)"/>
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67 <param name="prefix" type="integer" value="3" label="Prefix length used to build sub k-mer table (--prefix)"/>
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68 <param name="min_count" type="integer" value="2" label="Minimum multiplicity for filtering k-mer when building the graph (--min_count)"/>
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69 <param name="min_support" type="integer" value="1" label="Minimum supoort in each iteration (--min_support)"/>
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70 <param name="num_threads" type="integer" value="0" label="Number of threads (--num_threads)"/>
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71 <param name="seed_kmer" type="integer" value="30" label="Seed kmer size for alignment (--seed_kmer)"/>
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72 <param name="min_contig" type="integer" value="200" label="Minimum size of contig (--min_contig)"/>
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73 <param name="similar" type="float" value="0.95" label="Similarity for alignment (--similar)"/>
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74 <param name="max_mismatch" type="integer" value="3" label="Max mismatch of error correction (--max_mismatch)"/>
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75 <param name="min_pairs" type="integer" value="3" label="Minimum number of pairs (--min_pairs)"/>
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76
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77 <param name="other" type="select" display="checkboxes" separator=" " multiple="true" label="Other options">
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78 <option value="--no_bubble">Do not merge bubble (--no_bubble)</option>
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79 <option value="--no_local">Do not use local assembly (--no_local)</option>
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80 <option value="--no_coverage">Do not iterate on coverage (--no_coverage)</option>
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81 <option value="--no_correct">Do not do correction (--no_correct)</option>
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82 <option value="--pre_correction">Perform pre-correction before assembly (--pre_correction)</option>
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83 </param>
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84 </inputs>
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85
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86 <outputs>
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87 <data name="output" format="fasta"/>
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88 </outputs>
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89
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90 </tool>
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