# HG changeset patch # User tduigou # Date 1639758373 0 # Node ID 90025c4cd50e03db9473a657a755b35c4c16025d "planemo upload commit 2f1af427fa4c4f2aad53ab94c4cdb51456c66019-dirty" diff -r 000000000000 -r 90025c4cd50e dnaweaver.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/dnaweaver.xml Fri Dec 17 16:26:13 2021 +0000 @@ -0,0 +1,130 @@ + + Given a SBOL input, calculate assembly parts for Gibson or Golden Gate + + dnaweaver_synbiocad + + + + + + + + + + + + + +

+ +

+ + + + + + + + + + + + `_ + +Method +-------- + + - We assume that the different standard parts are available or will be ordered, with the exact sequence provided in the input file (in the future it would be easy to automatically break long parts into smaller fragments). + - The desired construct sequence for a genetic part design is simply the concatenation of that design's part sequences in the right order (no assembly overhang is included). + - Buy primers with overhangs to extend the parts fragments via PCR and create homologies between them so they can be assembled together. + - Assemble each construct in a single step with Golden Gate assembly if possible (that is if at least one site out of BsaI, BbsI, and BsmBI is totally absent from the construct sequence), else with Gibson assembly. It can also be only one of the two methods if the option Gibson or golden_gate is selected instead of any_method. + - Start with the first design, and for each subsequence design assembly plan reuse the primers ordered and fragments PCRed in previous designs, if relevant. + +Here is a schema of the supply network used: + +.. image:: https://raw.githubusercontent.com/brsynth/synbiocad-galaxy-wrappers/master/DNAWeaver/img/image4.png + :width: 30 % + :align: center + +| + +The output Excel spreadsheet contains the following sub-sheets: + + - construct_parts: the ID and list of part names (in the right order) for each design. + - construct_sequences: the final sequence of the constructs to build. + - part_sequences: the list of each standard part and its sequence (same information as in the input SBOL file). + - fragment_extensions: for each PCR fragment, the standard part and the primers to use + - assembly_plan: for each design, the list of PCR fragments to use. + - errors: list of errors to help troubleshooting assemblies for which no valid assembly plan was found. + +Limitations +-------------- + + +For constructs with repeated parts and other homologies (such as, in the example, the designs with several "Ter" in a row, ), Gibson assembly (and probably LCR assembly too) may create mis-annealed constructs and more clones will need to be picked. This is not taken into account by the script at the moment. This could be fixed by buying custom fragments from a commercial vendor for extreme cases (i.e. by amending the current implementation to forbid Gibson cuts in regions with homologies elsewhere and add a DNA vendor in the supply network). + +Version +------- + +1.0.1 + +Authors +------- + +* **Valentin Zulkower** +* Melchior du Lac + +License +------- + +`MIT `_ + +References +--------------- + +`DNA Weaver: optimal DNA assembly strategies via supply networks and shortest-path algorithms `_ + +Acknowledgments +--------------- + +* Thomas Duigou +* Joan Hérisson + + ]]> + diff -r 000000000000 -r 90025c4cd50e test-data/assembly_plan_lycopene.xlsx Binary file test-data/assembly_plan_lycopene.xlsx has changed diff -r 000000000000 -r 90025c4cd50e test-data/constructs_lycopene.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/constructs_lycopene.xml Fri Dec 17 16:26:13 2021 +0000 @@ -0,0 +1,8911 @@ + + + + P21683_10000_cds_seq + ATGAACAACCCGTCACTTTTGAATCATGCCGTGGAAACTATGGCAGTGGGCAGCAAAAGCTTTGCCACCGCGTCCAAACTATTCGATGCGAAAACGCGGCGCTCAGTACTTATGCTGTACGCGTGGTGCCGCCACTGTGATGATGTTATTGATGATCAAACATTGGGTTTTCAAGCCCGCCAGCCAGCTTTGCAGACCCCGGAACAGCGTCTTATGCAGCTGGAAATGAAAACCCGACAGGCCTATGCAGGGAGTCAGATGCACGAGCCGGCGTTTGCGGCGTTCCAGGAGGTGGCCATGGCCCATGATATCGCACCGGCGTACGCCTTCGATCATCTTGAAGGCTTTGCAATGGATGTGCGCGAAGCGCAATACTCGCAGCTGGACGACACCCTGCGGTATTGTTACCACGTAGCCGGTGTTGTCGGTCTGATGATGGCACAAATTATGGGGGTGCGCGACAACGCGACCTTGGATAGGGCCTGTGACCTGGGCCTAGCATTCCAGTTGACCAACATTGCGCGCGATATCGTGGATGATGCACACGCCGGTCGCTGCTACCTACCGGCGAGTTGGCTGGAACATGAAGGCCTGAATAAGGAAAATTATGCCGCCCCTGAAAACCGCCAGGCGCTGTCTCGCATTGCGCGTCGTCTTGTTCAGGAGGCCGAACCGTATTATCTTAGCGCGACCGCCGGGTTAGCCGGTCTGCCGCTGCGTAGCGCTTGGGCGATTGCCACCGCCAAGCAGGTATACCGTAAAATCGGCGTTAAAGTTGAACAGGCCGGCCAGCAAGCGTGGGATCAGCGCCAGAGCACGACCACCCCTGAAAAGTTGACGTTACTGCTGGCCGCGTCGGGCCAGGCGCTTACCAGCCGCATGCGCGCGCACCCACCGCGACCGGCTCATCTGTGGCAGCGCCCGCTG + + + 1 + + + P21683_10000_gene_seq + TGGGCCAGATATGATGTAGGAAGCGATCATTAGCCTGCGAATCCTGGAGTCTATGTGAGACTTGAATCTTAATTTTGGAGCTAAAAATGAACAACCCGTCACTTTTGAATCATGCCGTGGAAACTATGGCAGTGGGCAGCAAAAGCTTTGCCACCGCGTCCAAACTATTCGATGCGAAAACGCGGCGCTCAGTACTTATGCTGTACGCGTGGTGCCGCCACTGTGATGATGTTATTGATGATCAAACATTGGGTTTTCAAGCCCGCCAGCCAGCTTTGCAGACCCCGGAACAGCGTCTTATGCAGCTGGAAATGAAAACCCGACAGGCCTATGCAGGGAGTCAGATGCACGAGCCGGCGTTTGCGGCGTTCCAGGAGGTGGCCATGGCCCATGATATCGCACCGGCGTACGCCTTCGATCATCTTGAAGGCTTTGCAATGGATGTGCGCGAAGCGCAATACTCGCAGCTGGACGACACCCTGCGGTATTGTTACCACGTAGCCGGTGTTGTCGGTCTGATGATGGCACAAATTATGGGGGTGCGCGACAACGCGACCTTGGATAGGGCCTGTGACCTGGGCCTAGCATTCCAGTTGACCAACATTGCGCGCGATATCGTGGATGATGCACACGCCGGTCGCTGCTACCTACCGGCGAGTTGGCTGGAACATGAAGGCCTGAATAAGGAAAATTATGCCGCCCCTGAAAACCGCCAGGCGCTGTCTCGCATTGCGCGTCGTCTTGTTCAGGAGGCCGAACCGTATTATCTTAGCGCGACCGCCGGGTTAGCCGGTCTGCCGCTGCGTAGCGCTTGGGCGATTGCCACCGCCAAGCAGGTATACCGTAAAATCGGCGTTAAAGTTGAACAGGCCGGCCAGCAAGCGTGGGATCAGCGCCAGAGCACGACCACCCCTGAAAAGTTGACGTTACTGCTGGCCGCGTCGGGCCAGGCGCTTACCAGCCGCATGCGCGCGCACCCACCGCGACCGGCTCATCTGTGGCAGCGCCCGCTGTGACTGACTCTGACACTTTATAGAACTAGGCATGTAAGCCGAGCTTTTCTATGTTTAAAAGTGGCATCATTAGCCTGCGAATCCTGGAGTCTATGTGAGACTTGAATCTTAATTTTGGAGCTAAAAATGAACAACCCGTCACTTTTGAATCATGCCGTGGAAACTATGGCAGTGGGCAGCAAAAGCTTTGCCACCGCGTCCAAACTATTCGATGCGAAAACGCGGCGCTCAGTACTTATGCTGTACGCGTGGTGCCGCCACTGTGATGATGTTATTGATGATCAAACATTGGGTTTTCAAGCCCGCCAGCCAGCTTTGCAGACCCCGGAACAGCGTCTTATGCAGCTGGAAATGAAAACCCGACAGGCCTATGCAGGGAGTCAGATGCACGAGCCGGCGTTTGCGGCGTTCCAGGAGGTGGCCATGGCCCATGATATCGCACCGGCGTACGCCTTCGATCATCTTGAAGGCTTTGCAATGGATGTGCGCGAAGCGCAATACTCGCAGCTGGACGACACCCTGCGGTATTGTTACCACGTAGCCGGTGTTGTCGGTCTGATGATGGCACAAATTATGGGGGTGCGCGACAACGCGACCTTGGATAGGGCCTGTGACCTGGGCCTAGCATTCCAGTTGACCAACATTGCGCGCGATATCGTGGATGATGCACACGCCGGTCGCTGCTACCTACCGGCGAGTTGGCTGGAACATGAAGGCCTGAATAAGGAAAATTATGCCGCCCCTGAAAACCGCCAGGCGCTGTCTCGCATTGCGCGTCGTCTTGTTCAGGAGGCCGAACCGTATTATCTTAGCGCGACCGCCGGGTTAGCCGGTCTGCCGCTGCGTAGCGCTTGGGCGATTGCCACCGCCAAGCAGGTATACCGTAAAATCGGCGTTAAAGTTGAACAGGCCGGCCAGCAAGCGTGGGATCAGCGCCAGAGCACGACCACCCCTGAAAAGTTGACGTTACTGCTGGCCGCGTCGGGCCAGGCGCTTACCAGCCGCATGCGCGCGCACCCACCGCGACCGGCTCATCTGTGGCAGCGCCCGCTGAGTGAATCTAAACGTGTCTTTCTGGGAAGATCGGGGACATGGGGATCGATCACCAATAGCTCATTAGCCTGCGAATCCTGGAGTCTATGTGAGACTTGAATCTTAATTTTGGAGCTAAAAATGAACAACCCGTCACTTTTGAATCATGCCGTGGAAACTATGGCAGTGGGCAGCAAAAGCTTTGCCACCGCGTCCAAACTATTCGATGCGAAAACGCGGCGCTCAGTACTTATGCTGTACGCGTGGTGCCGCCACTGTGATGATGTTATTGATGATCAAACATTGGGTTTTCAAGCCCGCCAGCCAGCTTTGCAGACCCCGGAACAGCGTCTTATGCAGCTGGAAATGAAAACCCGACAGGCCTATGCAGGGAGTCAGATGCACGAGCCGGCGTTTGCGGCGTTCCAGGAGGTGGCCATGGCCCATGATATCGCACCGGCGTACGCCTTCGATCATCTTGAAGGCTTTGCAATGGATGTGCGCGAAGCGCAATACTCGCAGCTGGACGACACCCTGCGGTATTGTTACCACGTAGCCGGTGTTGTCGGTCTGATGATGGCACAAATTATGGGGGTGCGCGACAACGCGACCTTGGATAGGGCCTGTGACCTGGGCCTAGCATTCCAGTTGACCAACATTGCGCGCGATATCGTGGATGATGCACACGCCGGTCGCTGCTACCTACCGGCGAGTTGGCTGGAACATGAAGGCCTGAATAAGGAAAATTATGCCGCCCCTGAAAACCGCCAGGCGCTGTCTCGCATTGCGCGTCGTCTTGTTCAGGAGGCCGAACCGTATTATCTTAGCGCGACCGCCGGGTTAGCCGGTCTGCCGCTGCGTAGCGCTTGGGCGATTGCCACCGCCAAGCAGGTATACCGTAAAATCGGCGTTAAAGTTGAACAGGCCGGCCAGCAAGCGTGGGATCAGCGCCAGAGCACGACCACCCCTGAAAAGTTGACGTTACTGCTGGCCGCGTCGGGCCAGGCGCTTACCAGCCGCATGCGCGCGCACCCACCGCGACCGGCTCATCTGTGGCAGCGCCCGCTGTACGCCTGGAAAACATTACTGACCCCC + + + 1 + + + P21683_10000_rbs_seq + TCATTAGCCTGCGAATCCTGGAGTCTATGTGAGACTTGAATCTTAATTTTGGAGCTAAAA + + + 1 + + + P21683_20000_cds_seq + ATGAACAACCCAAGCCTGTTAAACCATGCCGTCGAAACGATGGCGGTCGGCAGCAAGAGCTTTGCCACGGCAAGTAAACTGTTTGACGCTAAAACACGTCGCAGCGTTTTAATGCTGTATGCCTGGTGTCGCCACTGTGACGATGTGATTGACGACCAGACCCTGGGCTTTCAGGCTCGCCAGCCCGCGTTACAGACGCCGGAACAGCGTCTCATGCAGCTCGAAATGAAGACGCGACAGGCTTATGCAGGTAGCCAAATGCACGAACCGGCATTCGCCGCGTTCCAAGAAGTTGCGATGGCGCATGACATTGCCCCAGCCTACGCTTTCGATCACCTCGAAGGTTTCGCAATGGACGTCCGCGAGGCCCAATATAGTCAGCTGGACGATACGCTCCGATACTGCTACCATGTTGCGGGAGTTGTAGGTCTTATGATGGCGCAGATAATGGGCGTCCGTGATAACGCGACCCTGGATCGTGCGTGTGATCTGGGCCTGGCGTTCCAACTGACTAACATAGCACGCGACATTGTGGATGATGCGCACGCGGGGCGCTGTTACCTCCCGGCGAGTTGGCTGGAGCATGAAGGACTTAACAAAGAAAACTATGCTGCTCCAGAAAATCGCCAGGCGCTCAGTCGAATTGCGCGTCGCCTGGTTCAGGAAGCAGAACCGTATTACCTCTCAGCGACCGCCGGCCTGGCGGGTTTACCCCTGCGTTCGGCCTGGGCAATTGCGACCGCGAAGCAGGTGTACAGGAAAATTGGCGTGAAGGTAGAACAGGCGGGCCAGCAAGCGTGGGATCAGCGTCAGAGCACTACTACCCCGGAAAAGTTGACGCTGTTGTTAGCCGCGAGCGGCCAGGCCCTGACCAGTCGCATGCGTGCTCATCCGCCCAGACCGGCACATCTATGGCAGCGCCCGCTG + + + 1 + + + P21683_20000_gene_seq + TGGGCCAGATATGATGTAGGAAGCGAATCTTTTGTTGCTCCAATCATAAGCGGCCACCAGCCACAAACACCTGAGGGAGTCTCCTTATGAACAACCCAAGCCTGTTAAACCATGCCGTCGAAACGATGGCGGTCGGCAGCAAGAGCTTTGCCACGGCAAGTAAACTGTTTGACGCTAAAACACGTCGCAGCGTTTTAATGCTGTATGCCTGGTGTCGCCACTGTGACGATGTGATTGACGACCAGACCCTGGGCTTTCAGGCTCGCCAGCCCGCGTTACAGACGCCGGAACAGCGTCTCATGCAGCTCGAAATGAAGACGCGACAGGCTTATGCAGGTAGCCAAATGCACGAACCGGCATTCGCCGCGTTCCAAGAAGTTGCGATGGCGCATGACATTGCCCCAGCCTACGCTTTCGATCACCTCGAAGGTTTCGCAATGGACGTCCGCGAGGCCCAATATAGTCAGCTGGACGATACGCTCCGATACTGCTACCATGTTGCGGGAGTTGTAGGTCTTATGATGGCGCAGATAATGGGCGTCCGTGATAACGCGACCCTGGATCGTGCGTGTGATCTGGGCCTGGCGTTCCAACTGACTAACATAGCACGCGACATTGTGGATGATGCGCACGCGGGGCGCTGTTACCTCCCGGCGAGTTGGCTGGAGCATGAAGGACTTAACAAAGAAAACTATGCTGCTCCAGAAAATCGCCAGGCGCTCAGTCGAATTGCGCGTCGCCTGGTTCAGGAAGCAGAACCGTATTACCTCTCAGCGACCGCCGGCCTGGCGGGTTTACCCCTGCGTTCGGCCTGGGCAATTGCGACCGCGAAGCAGGTGTACAGGAAAATTGGCGTGAAGGTAGAACAGGCGGGCCAGCAAGCGTGGGATCAGCGTCAGAGCACTACTACCCCGGAAAAGTTGACGCTGTTGTTAGCCGCGAGCGGCCAGGCCCTGACCAGTCGCATGCGTGCTCATCCGCCCAGACCGGCACATCTATGGCAGCGCCCGCTGTGACTGACTCTGACACTTTATAGAACTAGGCATGTAAGCCGAGCTTTTCTATGTTTAAAAGTGGCAATCTTTTGTTGCTCCAATCATAAGCGGCCACCAGCCACAAACACCTGAGGGAGTCTCCTTATGAACAACCCAAGCCTGTTAAACCATGCCGTCGAAACGATGGCGGTCGGCAGCAAGAGCTTTGCCACGGCAAGTAAACTGTTTGACGCTAAAACACGTCGCAGCGTTTTAATGCTGTATGCCTGGTGTCGCCACTGTGACGATGTGATTGACGACCAGACCCTGGGCTTTCAGGCTCGCCAGCCCGCGTTACAGACGCCGGAACAGCGTCTCATGCAGCTCGAAATGAAGACGCGACAGGCTTATGCAGGTAGCCAAATGCACGAACCGGCATTCGCCGCGTTCCAAGAAGTTGCGATGGCGCATGACATTGCCCCAGCCTACGCTTTCGATCACCTCGAAGGTTTCGCAATGGACGTCCGCGAGGCCCAATATAGTCAGCTGGACGATACGCTCCGATACTGCTACCATGTTGCGGGAGTTGTAGGTCTTATGATGGCGCAGATAATGGGCGTCCGTGATAACGCGACCCTGGATCGTGCGTGTGATCTGGGCCTGGCGTTCCAACTGACTAACATAGCACGCGACATTGTGGATGATGCGCACGCGGGGCGCTGTTACCTCCCGGCGAGTTGGCTGGAGCATGAAGGACTTAACAAAGAAAACTATGCTGCTCCAGAAAATCGCCAGGCGCTCAGTCGAATTGCGCGTCGCCTGGTTCAGGAAGCAGAACCGTATTACCTCTCAGCGACCGCCGGCCTGGCGGGTTTACCCCTGCGTTCGGCCTGGGCAATTGCGACCGCGAAGCAGGTGTACAGGAAAATTGGCGTGAAGGTAGAACAGGCGGGCCAGCAAGCGTGGGATCAGCGTCAGAGCACTACTACCCCGGAAAAGTTGACGCTGTTGTTAGCCGCGAGCGGCCAGGCCCTGACCAGTCGCATGCGTGCTCATCCGCCCAGACCGGCACATCTATGGCAGCGCCCGCTGAGTGAATCTAAACGTGTCTTTCTGGGAAGATCGGGGACATGGGGATCGATCACCAATAGCATCTTTTGTTGCTCCAATCATAAGCGGCCACCAGCCACAAACACCTGAGGGAGTCTCCTTATGAACAACCCAAGCCTGTTAAACCATGCCGTCGAAACGATGGCGGTCGGCAGCAAGAGCTTTGCCACGGCAAGTAAACTGTTTGACGCTAAAACACGTCGCAGCGTTTTAATGCTGTATGCCTGGTGTCGCCACTGTGACGATGTGATTGACGACCAGACCCTGGGCTTTCAGGCTCGCCAGCCCGCGTTACAGACGCCGGAACAGCGTCTCATGCAGCTCGAAATGAAGACGCGACAGGCTTATGCAGGTAGCCAAATGCACGAACCGGCATTCGCCGCGTTCCAAGAAGTTGCGATGGCGCATGACATTGCCCCAGCCTACGCTTTCGATCACCTCGAAGGTTTCGCAATGGACGTCCGCGAGGCCCAATATAGTCAGCTGGACGATACGCTCCGATACTGCTACCATGTTGCGGGAGTTGTAGGTCTTATGATGGCGCAGATAATGGGCGTCCGTGATAACGCGACCCTGGATCGTGCGTGTGATCTGGGCCTGGCGTTCCAACTGACTAACATAGCACGCGACATTGTGGATGATGCGCACGCGGGGCGCTGTTACCTCCCGGCGAGTTGGCTGGAGCATGAAGGACTTAACAAAGAAAACTATGCTGCTCCAGAAAATCGCCAGGCGCTCAGTCGAATTGCGCGTCGCCTGGTTCAGGAAGCAGAACCGTATTACCTCTCAGCGACCGCCGGCCTGGCGGGTTTACCCCTGCGTTCGGCCTGGGCAATTGCGACCGCGAAGCAGGTGTACAGGAAAATTGGCGTGAAGGTAGAACAGGCGGGCCAGCAAGCGTGGGATCAGCGTCAGAGCACTACTACCCCGGAAAAGTTGACGCTGTTGTTAGCCGCGAGCGGCCAGGCCCTGACCAGTCGCATGCGTGCTCATCCGCCCAGACCGGCACATCTATGGCAGCGCCCGCTGTACGCCTGGAAAACATTACTGACCCCC + + + 1 + + + P21683_20000_rbs_seq + ATCTTTTGTTGCTCCAATCATAAGCGGCCACCAGCCACAAACACCTGAGGGAGTCTCCTT + + + 1 + + + P21683_30000_cds_seq + ATGAACAACCCTTCCCTTTTAAATCACGCCGTCGAAACTATGGCCGTTGGCAGCAAATCTTTTGCGACTGCGAGTAAGCTGTTTGACGCGAAGACGCGCCGATCGGTGCTGATGCTGTATGCATGGTGCCGCCATTGCGACGATGTGATTGATGATCAGACTCTGGGTTTTCAGGCGCGCCAGCCGGCGTTACAGACACCGGAACAACGTCTGATGCAATTGGAGATGAAGACCCGGCAAGCATATGCAGGCAGCCAGATGCACGAACCCGCGTTTGCTGCATTTCAGGAAGTGGCAATGGCCCACGATATCGCCCCAGCGTATGCTTTTGATCATTTAGAAGGTTTCGCCATGGATGTGCGTGAGGCCCAGTACTCGCAGCTCGATGATACGCTGCGGTACTGCTATCATGTGGCCGGTGTGGTGGGACTGATGATGGCGCAAATCATGGGCGTGCGTGATAATGCGACTTTAGATAGGGCATGTGATCTGGGTCTAGCATTTCAGTTAACCAACATTGCACGAGATATTGTGGACGACGCCCATGCCGGTCGCTGCTATCTCCCAGCATCCTGGCTGGAGCATGAAGGGTTAAATAAAGAGAACTACGCGGCCCCTGAAAATCGCCAGGCACTGTCCCGGATCGCACGCCGCCTGGTGCAAGAAGCCGAACCTTATTACTTGTCGGCGACCGCAGGTCTGGCCGGGCTGCCGTTGCGTAGTGCCTGGGCGATTGCCACAGCAAAACAAGTGTATCGTAAAATTGGAGTAAAAGTAGAACAGGCTGGTCAGCAGGCTTGGGATCAACGTCAATCGACGACAACCCCCGAAAAATTAACGTTGCTGCTGGCGGCCAGCGGCCAGGCCCTGACGTCTCGCATGCGGGCTCATCCGCCGCGCCCGGCCCATCTGTGGCAGCGACCATTG + + + 1 + + + P21683_30000_gene_seq + TGGGCCAGATATGATGTAGGAAGCGAGGAATCACTAGTTTTGACGATCATAAACTGAGACTTCTCGCTAGCCACAGGAGCACACAGATGAACAACCCTTCCCTTTTAAATCACGCCGTCGAAACTATGGCCGTTGGCAGCAAATCTTTTGCGACTGCGAGTAAGCTGTTTGACGCGAAGACGCGCCGATCGGTGCTGATGCTGTATGCATGGTGCCGCCATTGCGACGATGTGATTGATGATCAGACTCTGGGTTTTCAGGCGCGCCAGCCGGCGTTACAGACACCGGAACAACGTCTGATGCAATTGGAGATGAAGACCCGGCAAGCATATGCAGGCAGCCAGATGCACGAACCCGCGTTTGCTGCATTTCAGGAAGTGGCAATGGCCCACGATATCGCCCCAGCGTATGCTTTTGATCATTTAGAAGGTTTCGCCATGGATGTGCGTGAGGCCCAGTACTCGCAGCTCGATGATACGCTGCGGTACTGCTATCATGTGGCCGGTGTGGTGGGACTGATGATGGCGCAAATCATGGGCGTGCGTGATAATGCGACTTTAGATAGGGCATGTGATCTGGGTCTAGCATTTCAGTTAACCAACATTGCACGAGATATTGTGGACGACGCCCATGCCGGTCGCTGCTATCTCCCAGCATCCTGGCTGGAGCATGAAGGGTTAAATAAAGAGAACTACGCGGCCCCTGAAAATCGCCAGGCACTGTCCCGGATCGCACGCCGCCTGGTGCAAGAAGCCGAACCTTATTACTTGTCGGCGACCGCAGGTCTGGCCGGGCTGCCGTTGCGTAGTGCCTGGGCGATTGCCACAGCAAAACAAGTGTATCGTAAAATTGGAGTAAAAGTAGAACAGGCTGGTCAGCAGGCTTGGGATCAACGTCAATCGACGACAACCCCCGAAAAATTAACGTTGCTGCTGGCGGCCAGCGGCCAGGCCCTGACGTCTCGCATGCGGGCTCATCCGCCGCGCCCGGCCCATCTGTGGCAGCGACCATTGTGACTGACTCTGACACTTTATAGAACTAGGCATGTAAGCCGAGCTTTTCTATGTTTAAAAGTGGCAGGAATCACTAGTTTTGACGATCATAAACTGAGACTTCTCGCTAGCCACAGGAGCACACAGATGAACAACCCTTCCCTTTTAAATCACGCCGTCGAAACTATGGCCGTTGGCAGCAAATCTTTTGCGACTGCGAGTAAGCTGTTTGACGCGAAGACGCGCCGATCGGTGCTGATGCTGTATGCATGGTGCCGCCATTGCGACGATGTGATTGATGATCAGACTCTGGGTTTTCAGGCGCGCCAGCCGGCGTTACAGACACCGGAACAACGTCTGATGCAATTGGAGATGAAGACCCGGCAAGCATATGCAGGCAGCCAGATGCACGAACCCGCGTTTGCTGCATTTCAGGAAGTGGCAATGGCCCACGATATCGCCCCAGCGTATGCTTTTGATCATTTAGAAGGTTTCGCCATGGATGTGCGTGAGGCCCAGTACTCGCAGCTCGATGATACGCTGCGGTACTGCTATCATGTGGCCGGTGTGGTGGGACTGATGATGGCGCAAATCATGGGCGTGCGTGATAATGCGACTTTAGATAGGGCATGTGATCTGGGTCTAGCATTTCAGTTAACCAACATTGCACGAGATATTGTGGACGACGCCCATGCCGGTCGCTGCTATCTCCCAGCATCCTGGCTGGAGCATGAAGGGTTAAATAAAGAGAACTACGCGGCCCCTGAAAATCGCCAGGCACTGTCCCGGATCGCACGCCGCCTGGTGCAAGAAGCCGAACCTTATTACTTGTCGGCGACCGCAGGTCTGGCCGGGCTGCCGTTGCGTAGTGCCTGGGCGATTGCCACAGCAAAACAAGTGTATCGTAAAATTGGAGTAAAAGTAGAACAGGCTGGTCAGCAGGCTTGGGATCAACGTCAATCGACGACAACCCCCGAAAAATTAACGTTGCTGCTGGCGGCCAGCGGCCAGGCCCTGACGTCTCGCATGCGGGCTCATCCGCCGCGCCCGGCCCATCTGTGGCAGCGACCATTGAGTGAATCTAAACGTGTCTTTCTGGGAAGATCGGGGACATGGGGATCGATCACCAATAGCGGAATCACTAGTTTTGACGATCATAAACTGAGACTTCTCGCTAGCCACAGGAGCACACAGATGAACAACCCTTCCCTTTTAAATCACGCCGTCGAAACTATGGCCGTTGGCAGCAAATCTTTTGCGACTGCGAGTAAGCTGTTTGACGCGAAGACGCGCCGATCGGTGCTGATGCTGTATGCATGGTGCCGCCATTGCGACGATGTGATTGATGATCAGACTCTGGGTTTTCAGGCGCGCCAGCCGGCGTTACAGACACCGGAACAACGTCTGATGCAATTGGAGATGAAGACCCGGCAAGCATATGCAGGCAGCCAGATGCACGAACCCGCGTTTGCTGCATTTCAGGAAGTGGCAATGGCCCACGATATCGCCCCAGCGTATGCTTTTGATCATTTAGAAGGTTTCGCCATGGATGTGCGTGAGGCCCAGTACTCGCAGCTCGATGATACGCTGCGGTACTGCTATCATGTGGCCGGTGTGGTGGGACTGATGATGGCGCAAATCATGGGCGTGCGTGATAATGCGACTTTAGATAGGGCATGTGATCTGGGTCTAGCATTTCAGTTAACCAACATTGCACGAGATATTGTGGACGACGCCCATGCCGGTCGCTGCTATCTCCCAGCATCCTGGCTGGAGCATGAAGGGTTAAATAAAGAGAACTACGCGGCCCCTGAAAATCGCCAGGCACTGTCCCGGATCGCACGCCGCCTGGTGCAAGAAGCCGAACCTTATTACTTGTCGGCGACCGCAGGTCTGGCCGGGCTGCCGTTGCGTAGTGCCTGGGCGATTGCCACAGCAAAACAAGTGTATCGTAAAATTGGAGTAAAAGTAGAACAGGCTGGTCAGCAGGCTTGGGATCAACGTCAATCGACGACAACCCCCGAAAAATTAACGTTGCTGCTGGCGGCCAGCGGCCAGGCCCTGACGTCTCGCATGCGGGCTCATCCGCCGCGCCCGGCCCATCTGTGGCAGCGACCATTGTACGCCTGGAAAACATTACTGACCCCC + + + 1 + + + P21683_30000_rbs_seq + GGAATCACTAGTTTTGACGATCATAAACTGAGACTTCTCGCTAGCCACAGGAGCACACAG + + + 1 + + + P21684_10000_cds_seq + ATGACAGTTTGTGCCAAGAAACACGTGCACCTGACGCGTGATGCGGCCGAACAGTTATTAGCAGATATTGACCGTCGTCTGGACCAACTGCTGCCAGTCGAGGGCGAACGGGATGTTGTCGGAGCGGCAATGCGGGAAGGCGCATTGGCGCCGGGGAAACGCATCCGCCCGATGCTGCTGTTACTGACTGCGCGGGATTTAGGTTGTGCCGTGTCACACGACGGACTGCTGGATCTGGCATGCGCCGTAGAAATGGTGCATGCGGCGAGTCTGATCCTTGACGATATGCCGTGTATGGACGATGCAAAACTGCGCCGTGGCCGTCCGACTATCCACTCCCATTACGGCGAACATGTAGCCATCCTCGCGGCCGTGGCGTTGCTGTCCAAAGCGTTCGGCGTGATTGCTGATGCCGACGGTCTCACCCCGCTGGCCAAAAATCGTGCCGTTTCAGAACTGAGCAACGCAATTGGCATGCAAGGTTTAGTCCAGGGCCAGTTTAAAGATTTAAGTGAAGGTGATAAACCGCGTTCGGCCGAAGCCATCCTGATGACTAACCATTTCAAAACCTCGACCCTGTTCTGCGCCAGTATGCAGATGGCCAGCATCGTGGCCAACGCCTCTTCAGAAGCCCGGGATTGCTTACATCGATTTAGCCTGGATCTAGGCCAAGCGTTCCAACTTCTGGATGATTTAACAGATGGCATGACCGATACGGGGAAAGATAGCAACCAAGATGCGGGCAAAAGTACCCTGGTGAATTTGCTGGGACCGCGTGCCGTTGAAGAGCGCTTACGACAGCATCTCCAGCTGGCAAGCGAGCATTTATCAGCCGCATGTCAGCATGGACACGCAACGCAACACTTTATTCAAGCGTGGTTCGATAAAAAGCTGGCGGCGGTGTCC + + + 1 + + + P21684_10000_gene_seq + TGGGCCAGATATGATGTAGGAAGCGATTGCGACTGTGGCTTTCCGGTATCACTCTTTAACTTTTCTAAATCCGGGAGGCTATCAGAATGACAGTTTGTGCCAAGAAACACGTGCACCTGACGCGTGATGCGGCCGAACAGTTATTAGCAGATATTGACCGTCGTCTGGACCAACTGCTGCCAGTCGAGGGCGAACGGGATGTTGTCGGAGCGGCAATGCGGGAAGGCGCATTGGCGCCGGGGAAACGCATCCGCCCGATGCTGCTGTTACTGACTGCGCGGGATTTAGGTTGTGCCGTGTCACACGACGGACTGCTGGATCTGGCATGCGCCGTAGAAATGGTGCATGCGGCGAGTCTGATCCTTGACGATATGCCGTGTATGGACGATGCAAAACTGCGCCGTGGCCGTCCGACTATCCACTCCCATTACGGCGAACATGTAGCCATCCTCGCGGCCGTGGCGTTGCTGTCCAAAGCGTTCGGCGTGATTGCTGATGCCGACGGTCTCACCCCGCTGGCCAAAAATCGTGCCGTTTCAGAACTGAGCAACGCAATTGGCATGCAAGGTTTAGTCCAGGGCCAGTTTAAAGATTTAAGTGAAGGTGATAAACCGCGTTCGGCCGAAGCCATCCTGATGACTAACCATTTCAAAACCTCGACCCTGTTCTGCGCCAGTATGCAGATGGCCAGCATCGTGGCCAACGCCTCTTCAGAAGCCCGGGATTGCTTACATCGATTTAGCCTGGATCTAGGCCAAGCGTTCCAACTTCTGGATGATTTAACAGATGGCATGACCGATACGGGGAAAGATAGCAACCAAGATGCGGGCAAAAGTACCCTGGTGAATTTGCTGGGACCGCGTGCCGTTGAAGAGCGCTTACGACAGCATCTCCAGCTGGCAAGCGAGCATTTATCAGCCGCATGTCAGCATGGACACGCAACGCAACACTTTATTCAAGCGTGGTTCGATAAAAAGCTGGCGGCGGTGTCCTGACTGACTCTGACACTTTATAGAACTAGGCATGTAAGCCGAGCTTTTCTATGTTTAAAAGTGGCATTGCGACTGTGGCTTTCCGGTATCACTCTTTAACTTTTCTAAATCCGGGAGGCTATCAGAATGACAGTTTGTGCCAAGAAACACGTGCACCTGACGCGTGATGCGGCCGAACAGTTATTAGCAGATATTGACCGTCGTCTGGACCAACTGCTGCCAGTCGAGGGCGAACGGGATGTTGTCGGAGCGGCAATGCGGGAAGGCGCATTGGCGCCGGGGAAACGCATCCGCCCGATGCTGCTGTTACTGACTGCGCGGGATTTAGGTTGTGCCGTGTCACACGACGGACTGCTGGATCTGGCATGCGCCGTAGAAATGGTGCATGCGGCGAGTCTGATCCTTGACGATATGCCGTGTATGGACGATGCAAAACTGCGCCGTGGCCGTCCGACTATCCACTCCCATTACGGCGAACATGTAGCCATCCTCGCGGCCGTGGCGTTGCTGTCCAAAGCGTTCGGCGTGATTGCTGATGCCGACGGTCTCACCCCGCTGGCCAAAAATCGTGCCGTTTCAGAACTGAGCAACGCAATTGGCATGCAAGGTTTAGTCCAGGGCCAGTTTAAAGATTTAAGTGAAGGTGATAAACCGCGTTCGGCCGAAGCCATCCTGATGACTAACCATTTCAAAACCTCGACCCTGTTCTGCGCCAGTATGCAGATGGCCAGCATCGTGGCCAACGCCTCTTCAGAAGCCCGGGATTGCTTACATCGATTTAGCCTGGATCTAGGCCAAGCGTTCCAACTTCTGGATGATTTAACAGATGGCATGACCGATACGGGGAAAGATAGCAACCAAGATGCGGGCAAAAGTACCCTGGTGAATTTGCTGGGACCGCGTGCCGTTGAAGAGCGCTTACGACAGCATCTCCAGCTGGCAAGCGAGCATTTATCAGCCGCATGTCAGCATGGACACGCAACGCAACACTTTATTCAAGCGTGGTTCGATAAAAAGCTGGCGGCGGTGTCCAGTGAATCTAAACGTGTCTTTCTGGGAAGATCG + + + 1 + + + P21684_10000_rbs_seq + TTGCGACTGTGGCTTTCCGGTATCACTCTTTAACTTTTCTAAATCCGGGAGGCTATCAGA + + + 1 + + + P21684_20000_cds_seq + ATGACCGTCTGCGCGAAGAAACATGTCCATTTAACTCGTGACGCCGCGGAGCAGTTACTGGCGGATATAGATCGTCGTTTAGACCAGCTTCTGCCCGTCGAAGGAGAACGCGATGTGGTGGGGGCAGCCATGCGTGAAGGCGCCCTGGCCCCGGGTAAACGTATTCGCCCGATGCTGCTCTTATTAACCGCGCGTGATCTGGGCTGCGCAGTCTCGCATGATGGATTGTTAGACCTGGCGTGTGCTGTAGAGATGGTCCACGCTGCGTCGCTGATTTTGGATGACATGCCGTGCATGGATGATGCCAAGCTGCGCCGTGGTCGACCTACTATTCACTCACATTACGGCGAACACGTGGCAATCCTTGCGGCGGTGGCGCTGCTGAGCAAAGCGTTTGGCGTGATTGCTGATGCAGACGGTCTGACCCCTCTAGCTAAGAATCGCGCGGTGTCAGAGCTGTCAAATGCTATCGGCATGCAGGGGTTGGTGCAGGGCCAGTTCAAAGATCTGTCTGAAGGCGACAAGCCACGCTCAGCCGAAGCCATTCTGATGACGAATCACTTCAAGACAAGTACGTTATTCTGTGCGAGCATGCAAATGGCTTCAATCGTGGCCAATGCCTCTTCCGAAGCGCGCGATTGTCTGCATCGCTTTAGTCTGGATCTGGGCCAGGCGTTTCAACTGTTAGATGACCTAACAGATGGCATGACAGACACAGGCAAAGATTCGAATCAGGATGCTGGTAAGAGTACCCTGGTCAACCTGCTGGGTCCACGTGCGGTGGAAGAGCGGCTCCGCCAGCATTTACAGTTAGCGTCCGAACATCTGAGCGCGGCATGCCAACATGGTCACGCCACCCAGCATTTTATCCAAGCATGGTTTGACAAGAAGCTCGCGGCAGTATCT + + + 1 + + + P21684_20000_gene_seq + TGGGCCAGATATGATGTAGGAAGCGAACACATGACGTGACGAGTACCGGACACTGCTTGACCCAAGCAGACATTCAGGGAGGAGTGATGACCGTCTGCGCGAAGAAACATGTCCATTTAACTCGTGACGCCGCGGAGCAGTTACTGGCGGATATAGATCGTCGTTTAGACCAGCTTCTGCCCGTCGAAGGAGAACGCGATGTGGTGGGGGCAGCCATGCGTGAAGGCGCCCTGGCCCCGGGTAAACGTATTCGCCCGATGCTGCTCTTATTAACCGCGCGTGATCTGGGCTGCGCAGTCTCGCATGATGGATTGTTAGACCTGGCGTGTGCTGTAGAGATGGTCCACGCTGCGTCGCTGATTTTGGATGACATGCCGTGCATGGATGATGCCAAGCTGCGCCGTGGTCGACCTACTATTCACTCACATTACGGCGAACACGTGGCAATCCTTGCGGCGGTGGCGCTGCTGAGCAAAGCGTTTGGCGTGATTGCTGATGCAGACGGTCTGACCCCTCTAGCTAAGAATCGCGCGGTGTCAGAGCTGTCAAATGCTATCGGCATGCAGGGGTTGGTGCAGGGCCAGTTCAAAGATCTGTCTGAAGGCGACAAGCCACGCTCAGCCGAAGCCATTCTGATGACGAATCACTTCAAGACAAGTACGTTATTCTGTGCGAGCATGCAAATGGCTTCAATCGTGGCCAATGCCTCTTCCGAAGCGCGCGATTGTCTGCATCGCTTTAGTCTGGATCTGGGCCAGGCGTTTCAACTGTTAGATGACCTAACAGATGGCATGACAGACACAGGCAAAGATTCGAATCAGGATGCTGGTAAGAGTACCCTGGTCAACCTGCTGGGTCCACGTGCGGTGGAAGAGCGGCTCCGCCAGCATTTACAGTTAGCGTCCGAACATCTGAGCGCGGCATGCCAACATGGTCACGCCACCCAGCATTTTATCCAAGCATGGTTTGACAAGAAGCTCGCGGCAGTATCTTGACTGACTCTGACACTTTATAGAACTAGGCATGTAAGCCGAGCTTTTCTATGTTTAAAAGTGGCAACACATGACGTGACGAGTACCGGACACTGCTTGACCCAAGCAGACATTCAGGGAGGAGTGATGACCGTCTGCGCGAAGAAACATGTCCATTTAACTCGTGACGCCGCGGAGCAGTTACTGGCGGATATAGATCGTCGTTTAGACCAGCTTCTGCCCGTCGAAGGAGAACGCGATGTGGTGGGGGCAGCCATGCGTGAAGGCGCCCTGGCCCCGGGTAAACGTATTCGCCCGATGCTGCTCTTATTAACCGCGCGTGATCTGGGCTGCGCAGTCTCGCATGATGGATTGTTAGACCTGGCGTGTGCTGTAGAGATGGTCCACGCTGCGTCGCTGATTTTGGATGACATGCCGTGCATGGATGATGCCAAGCTGCGCCGTGGTCGACCTACTATTCACTCACATTACGGCGAACACGTGGCAATCCTTGCGGCGGTGGCGCTGCTGAGCAAAGCGTTTGGCGTGATTGCTGATGCAGACGGTCTGACCCCTCTAGCTAAGAATCGCGCGGTGTCAGAGCTGTCAAATGCTATCGGCATGCAGGGGTTGGTGCAGGGCCAGTTCAAAGATCTGTCTGAAGGCGACAAGCCACGCTCAGCCGAAGCCATTCTGATGACGAATCACTTCAAGACAAGTACGTTATTCTGTGCGAGCATGCAAATGGCTTCAATCGTGGCCAATGCCTCTTCCGAAGCGCGCGATTGTCTGCATCGCTTTAGTCTGGATCTGGGCCAGGCGTTTCAACTGTTAGATGACCTAACAGATGGCATGACAGACACAGGCAAAGATTCGAATCAGGATGCTGGTAAGAGTACCCTGGTCAACCTGCTGGGTCCACGTGCGGTGGAAGAGCGGCTCCGCCAGCATTTACAGTTAGCGTCCGAACATCTGAGCGCGGCATGCCAACATGGTCACGCCACCCAGCATTTTATCCAAGCATGGTTTGACAAGAAGCTCGCGGCAGTATCTAGTGAATCTAAACGTGTCTTTCTGGGAAGATCG + + + 1 + + + P21684_20000_rbs_seq + ACACATGACGTGACGAGTACCGGACACTGCTTGACCCAAGCAGACATTCAGGGAGGAGTG + + + 1 + + + P21684_30000_cds_seq + ATGACCGTCTGCGCAAAGAAACACGTCCACCTGACACGCGATGCCGCAGAGCAGCTGTTGGCGGATATAGATCGTCGCCTGGATCAGCTGTTGCCGGTGGAAGGCGAACGCGACGTTGTGGGAGCTGCCATGCGTGAAGGCGCGTTGGCTCCGGGTAAACGTATCCGCCCGATGCTGTTACTTCTGACCGCTCGCGATCTTGGCTGCGCAGTATCACATGATGGTTTGTTAGACCTGGCGTGCGCCGTTGAAATGGTACACGCAGCCTCCTTGATTTTGGACGATATGCCCTGCATGGACGATGCGAAGCTTCGCCGTGGCCGCCCGACCATTCACAGCCACTACGGCGAACACGTAGCGATTTTGGCAGCCGTAGCATTGTTGAGCAAAGCTTTCGGCGTTATTGCCGATGCAGACGGGTTAACTCCTCTGGCGAAGAATCGCGCTGTGTCAGAGCTGTCAAACGCTATCGGCATGCAGGGATTGGTACAGGGCCAGTTCAAAGATCTGAGTGAAGGCGATAAACCTCGCTCAGCCGAAGCGATTTTAATGACGAATCACTTTAAAACGTCCACTCTGTTTTGCGCGTCGATGCAAATGGCCTCCATTGTTGCAAACGCCAGCTCCGAAGCCCGGGATTGCCTCCATCGATTTTCGCTCGATCTGGGCCAGGCTTTTCAGCTGTTAGATGACTTAACGGATGGTATGACGGATACCGGCAAAGATAGTAATCAGGATGCAGGAAAAAGCACGCTGGTCAATCTCCTTGGACCTAGGGCCGTGGAAGAACGTCTGCGTCAACACCTGCAGCTGGCATCCGAACATCTGAGCGCCGCTTGCCAACACGGACACGCGACTCAGCATTTCATTCAGGCCTGGTTTGATAAGAAACTGGCAGCCGTTAGC + + + 1 + + + P21684_30000_gene_seq + TGGGCCAGATATGATGTAGGAAGCGATTCAGATCAGCCAAGGTCACGTTCGTACGATGTATACAGTACACACACAAGGAGAGAGCAATGACCGTCTGCGCAAAGAAACACGTCCACCTGACACGCGATGCCGCAGAGCAGCTGTTGGCGGATATAGATCGTCGCCTGGATCAGCTGTTGCCGGTGGAAGGCGAACGCGACGTTGTGGGAGCTGCCATGCGTGAAGGCGCGTTGGCTCCGGGTAAACGTATCCGCCCGATGCTGTTACTTCTGACCGCTCGCGATCTTGGCTGCGCAGTATCACATGATGGTTTGTTAGACCTGGCGTGCGCCGTTGAAATGGTACACGCAGCCTCCTTGATTTTGGACGATATGCCCTGCATGGACGATGCGAAGCTTCGCCGTGGCCGCCCGACCATTCACAGCCACTACGGCGAACACGTAGCGATTTTGGCAGCCGTAGCATTGTTGAGCAAAGCTTTCGGCGTTATTGCCGATGCAGACGGGTTAACTCCTCTGGCGAAGAATCGCGCTGTGTCAGAGCTGTCAAACGCTATCGGCATGCAGGGATTGGTACAGGGCCAGTTCAAAGATCTGAGTGAAGGCGATAAACCTCGCTCAGCCGAAGCGATTTTAATGACGAATCACTTTAAAACGTCCACTCTGTTTTGCGCGTCGATGCAAATGGCCTCCATTGTTGCAAACGCCAGCTCCGAAGCCCGGGATTGCCTCCATCGATTTTCGCTCGATCTGGGCCAGGCTTTTCAGCTGTTAGATGACTTAACGGATGGTATGACGGATACCGGCAAAGATAGTAATCAGGATGCAGGAAAAAGCACGCTGGTCAATCTCCTTGGACCTAGGGCCGTGGAAGAACGTCTGCGTCAACACCTGCAGCTGGCATCCGAACATCTGAGCGCCGCTTGCCAACACGGACACGCGACTCAGCATTTCATTCAGGCCTGGTTTGATAAGAAACTGGCAGCCGTTAGCTGACTGACTCTGACACTTTATAGAACTAGGCATGTAAGCCGAGCTTTTCTATGTTTAAAAGTGGCATTCAGATCAGCCAAGGTCACGTTCGTACGATGTATACAGTACACACACAAGGAGAGAGCAATGACCGTCTGCGCAAAGAAACACGTCCACCTGACACGCGATGCCGCAGAGCAGCTGTTGGCGGATATAGATCGTCGCCTGGATCAGCTGTTGCCGGTGGAAGGCGAACGCGACGTTGTGGGAGCTGCCATGCGTGAAGGCGCGTTGGCTCCGGGTAAACGTATCCGCCCGATGCTGTTACTTCTGACCGCTCGCGATCTTGGCTGCGCAGTATCACATGATGGTTTGTTAGACCTGGCGTGCGCCGTTGAAATGGTACACGCAGCCTCCTTGATTTTGGACGATATGCCCTGCATGGACGATGCGAAGCTTCGCCGTGGCCGCCCGACCATTCACAGCCACTACGGCGAACACGTAGCGATTTTGGCAGCCGTAGCATTGTTGAGCAAAGCTTTCGGCGTTATTGCCGATGCAGACGGGTTAACTCCTCTGGCGAAGAATCGCGCTGTGTCAGAGCTGTCAAACGCTATCGGCATGCAGGGATTGGTACAGGGCCAGTTCAAAGATCTGAGTGAAGGCGATAAACCTCGCTCAGCCGAAGCGATTTTAATGACGAATCACTTTAAAACGTCCACTCTGTTTTGCGCGTCGATGCAAATGGCCTCCATTGTTGCAAACGCCAGCTCCGAAGCCCGGGATTGCCTCCATCGATTTTCGCTCGATCTGGGCCAGGCTTTTCAGCTGTTAGATGACTTAACGGATGGTATGACGGATACCGGCAAAGATAGTAATCAGGATGCAGGAAAAAGCACGCTGGTCAATCTCCTTGGACCTAGGGCCGTGGAAGAACGTCTGCGTCAACACCTGCAGCTGGCATCCGAACATCTGAGCGCCGCTTGCCAACACGGACACGCGACTCAGCATTTCATTCAGGCCTGGTTTGATAAGAAACTGGCAGCCGTTAGCAGTGAATCTAAACGTGTCTTTCTGGGAAGATCG + + + 1 + + + P21684_30000_rbs_seq + TTCAGATCAGCCAAGGTCACGTTCGTACGATGTATACAGTACACACACAAGGAGAGAGCA + + + 1 + + + P21685_10000_cds_seq + ATGAAGCCAACCACGGTAATTGGTGCCGGTTTTGGCGGGTTAGCCCTGGCCATCCGCCTGCAAGCGGCAGGGATTCCTGTGCTTTTGTTGGAACAGCGCGATAAGCCGGGCGGCCGTGCCTACGTTTACGAAGATCAGGGCTTCACCTTTGATGCGGGCCCGACGGTGATCACTGATCCTTCAGCAATCGAAGAACTCTTTGCCCTGGCAGGGAAACAACTTAAAGAGTATGTGGAATTGCTACCAGTCACCCCGTTTTACCGCCTGTGCTGGGAAAGCGGCAAAGTGTTTAATTATGATAACGATCAGACCCGCCTGGAGGCTCAGATTCAACAATTTAATCCGCGTGACGTTGAGGGGTATCGCCAGTTTCTGGATTATTCACGTGCGGTCTTCAAAGAGGGATATCTGAAACTCGGCACAGTTCCATTTCTGAGCTTTCGTGATATGCTCCGTGCAGCACCGCAGCTGGCAAAACTGCAGGCCTGGCGTAGCGTTTATAGCAAAGTCGCGTCGTATATCGAGGATGAGCACCTGCGCCAGGCATTTAGTTTTCACAGTCTGCTGGTGGGCGGCAATCCGTTCGCTACGAGTTCGATTTACACGTTGATTCATGCTCTGGAGCGTGAATGGGGCGTATGGTTCCCGCGCGGTGGCACCGGGGCGCTTGTACAGGGCATGATTAAACTCTTCCAGGATCTCGGTGGCGAAGTGGTGCTGAATGCGCGGGTGTCACACATGGAGACCACTGGTAATAAAATTGAAGCCGTACATTTAGAAGACGGCCGCAGGTTCCTGACACAGGCGGTAGCTTCTAACGCAGATGTTGTGCATACCTACCGAGACCTGCTATCACAGCATCCGGCGGCGGTGAAACAGAGCAACAAGCTCCAGACTAAACGTATGTCCAACAGCCTTTTCGTGCTGTACTTTGGGCTGAACCACCATCATGATCAGTTAGCACATCATACGGTGTGTTTCGGGCCACGGTACCGCGAACTTATTGACGAGATTTTTAATCACGATGGTCTAGCCGAAGATTTCTCTCTGTACCTGCACGCCCCGTGCGTGACCGATTCGAGCCTAGCTCCAGAAGGTTGCGGTAGTTATTACGTTCTCGCCCCGGTGCCCCATCTCGGGACTGCAAACCTGGATTGGACTGTAGAAGGCCCCAAGCTGCGCGACCGAATTTTTGCCTACCTTGAGCAGCACTACATGCCTGGCCTTCGATCTCAGTTAGTAACTCACCGCATGTTCACCCCATTCGACTTCCGCGATCAGCTGAACGCGTACCATGGCAGCGCGTTTAGCGTCGAACCGGTGCTTACGCAGAGCGCATGGTTTCGACCGCATAACCGAGATAAGACGATCACGAATCTGTATTTAGTAGGCGCGGGCACCCATCCTGGCGCGGGAATCCCAGGCGTGATCGGCAGTGCAAAAGCCACGGCGGGCCTGATGCTGGAGGATCTCATC + + + 1 + + + P21685_10000_gene_seq + GGGACATGGGGATCGATCACCAATAGCCAGAGGGGCGTAATTACTGCGCCCCGAGTCATTAACTCAAACTGCGTGAGGGAGGTTATTATGAAGCCAACCACGGTAATTGGTGCCGGTTTTGGCGGGTTAGCCCTGGCCATCCGCCTGCAAGCGGCAGGGATTCCTGTGCTTTTGTTGGAACAGCGCGATAAGCCGGGCGGCCGTGCCTACGTTTACGAAGATCAGGGCTTCACCTTTGATGCGGGCCCGACGGTGATCACTGATCCTTCAGCAATCGAAGAACTCTTTGCCCTGGCAGGGAAACAACTTAAAGAGTATGTGGAATTGCTACCAGTCACCCCGTTTTACCGCCTGTGCTGGGAAAGCGGCAAAGTGTTTAATTATGATAACGATCAGACCCGCCTGGAGGCTCAGATTCAACAATTTAATCCGCGTGACGTTGAGGGGTATCGCCAGTTTCTGGATTATTCACGTGCGGTCTTCAAAGAGGGATATCTGAAACTCGGCACAGTTCCATTTCTGAGCTTTCGTGATATGCTCCGTGCAGCACCGCAGCTGGCAAAACTGCAGGCCTGGCGTAGCGTTTATAGCAAAGTCGCGTCGTATATCGAGGATGAGCACCTGCGCCAGGCATTTAGTTTTCACAGTCTGCTGGTGGGCGGCAATCCGTTCGCTACGAGTTCGATTTACACGTTGATTCATGCTCTGGAGCGTGAATGGGGCGTATGGTTCCCGCGCGGTGGCACCGGGGCGCTTGTACAGGGCATGATTAAACTCTTCCAGGATCTCGGTGGCGAAGTGGTGCTGAATGCGCGGGTGTCACACATGGAGACCACTGGTAATAAAATTGAAGCCGTACATTTAGAAGACGGCCGCAGGTTCCTGACACAGGCGGTAGCTTCTAACGCAGATGTTGTGCATACCTACCGAGACCTGCTATCACAGCATCCGGCGGCGGTGAAACAGAGCAACAAGCTCCAGACTAAACGTATGTCCAACAGCCTTTTCGTGCTGTACTTTGGGCTGAACCACCATCATGATCAGTTAGCACATCATACGGTGTGTTTCGGGCCACGGTACCGCGAACTTATTGACGAGATTTTTAATCACGATGGTCTAGCCGAAGATTTCTCTCTGTACCTGCACGCCCCGTGCGTGACCGATTCGAGCCTAGCTCCAGAAGGTTGCGGTAGTTATTACGTTCTCGCCCCGGTGCCCCATCTCGGGACTGCAAACCTGGATTGGACTGTAGAAGGCCCCAAGCTGCGCGACCGAATTTTTGCCTACCTTGAGCAGCACTACATGCCTGGCCTTCGATCTCAGTTAGTAACTCACCGCATGTTCACCCCATTCGACTTCCGCGATCAGCTGAACGCGTACCATGGCAGCGCGTTTAGCGTCGAACCGGTGCTTACGCAGAGCGCATGGTTTCGACCGCATAACCGAGATAAGACGATCACGAATCTGTATTTAGTAGGCGCGGGCACCCATCCTGGCGCGGGAATCCCAGGCGTGATCGGCAGTGCAAAAGCCACGGCGGGCCTGATGCTGGAGGATCTCATCTACGCCTGGAAAACATTACTGACCCCC + + + 1 + + + P21685_10000_rbs_seq + CAGAGGGGCGTAATTACTGCGCCCCGAGTCATTAACTCAAACTGCGTGAGGGAGGTTATT + + + 1 + + + P21685_20000_cds_seq + ATGAAACCAACGACCGTAATCGGCGCCGGCTTTGGGGGTTTGGCGCTTGCGATTCGCCTGCAGGCAGCGGGGATTCCGGTCTTGCTGCTCGAGCAGCGTGATAAACCGGGCGGCCGCGCTTATGTATATGAAGATCAGGGCTTTACCTTTGATGCAGGCCCGACGGTGATTACGGATCCGAGCGCGATAGAAGAACTTTTCGCCCTGGCCGGCAAACAACTGAAAGAGTATGTGGAGTTGCTGCCCGTAACCCCTTTCTATCGGTTGTGCTGGGAATCCGGTAAGGTGTTCAATTATGACAACGATCAAACCCGTCTGGAAGCCCAGATTCAACAGTTTAACCCGCGTGACGTTGAAGGCTATCGGCAGTTCCTTGACTACAGTCGCGCTGTCTTTAAAGAGGGTTACTTAAAACTGGGCACCGTGCCGTTCTTGTCGTTCCGCGATATGCTACGCGCAGCGCCTCAATTAGCCAAGCTGCAGGCATGGCGTTCCGTGTATAGCAAAGTCGCGAGTTATATTGAGGATGAACACCTGCGCCAGGCCTTTTCTTTTCATAGTCTGTTGGTAGGTGGCAACCCGTTCGCCACGTCCTCAATCTACACCCTGATCCATGCGCTGGAGCGTGAATGGGGCGTGTGGTTCCCGCGCGGGGGCACGGGGGCCCTGGTGCAGGGCATGATCAAACTGTTCCAGGACCTGGGCGGAGAAGTGGTTTTGAATGCCCGCGTGTCGCATATGGAAACGACAGGCAATAAAATTGAAGCGGTGCATTTAGAAGACGGCCGTCGCTTTCTGACCCAGGCTGTTGCCTCGAACGCGGATGTGGTACACACCTATCGGGATCTGCTCAGCCAGCATCCGGCAGCCGTAAAACAGTCTAACAAACTGCAAACGAAACGTATGAGCAACTCACTGTTCGTTCTATATTTTGGCCTGAACCACCACCACGACCAACTGGCACATCACACGGTTTGTTTTGGGCCGCGGTATCGCGAATTAATTGATGAAATTTTTAATCATGACGGGTTGGCGGAAGATTTTTCCCTGTATTTGCACGCGCCGTGTGTGACCGATAGCAGTTTGGCCCCGGAAGGTTGCGGGAGCTACTATGTACTGGCGCCGGTGCCTCATCTTGGAACCGCAAACCTGGACTGGACCGTGGAAGGCCCAAAACTCCGCGACCGTATATTTGCGTATCTGGAACAACACTACATGCCCGGCCTCCGCAGTCAACTGGTGACTCACCGCATGTTTACCCCGTTTGATTTTCGTGATCAGCTGAATGCTTACCATGGAAGTGCATTTAGCGTGGAACCAGTACTGACACAATCCGCCTGGTTCCGCCCGCATAATAGGGATAAAACCATAACCAATCTCTATCTGGTGGGTGCTGGCACTCATCCGGGCGCTGGTATTCCGGGCGTAATCGGTAGCGCCAAAGCTACCGCAGGGTTGATGCTTGAGGATCTGATT + + + 1 + + + P21685_20000_gene_seq + GGGACATGGGGATCGATCACCAATAGCTCCGGATTCGTCACGCTCGTTCGTGCTGGTAGACTTTTATAACAGGAAGGAGGAAGACACATGAAACCAACGACCGTAATCGGCGCCGGCTTTGGGGGTTTGGCGCTTGCGATTCGCCTGCAGGCAGCGGGGATTCCGGTCTTGCTGCTCGAGCAGCGTGATAAACCGGGCGGCCGCGCTTATGTATATGAAGATCAGGGCTTTACCTTTGATGCAGGCCCGACGGTGATTACGGATCCGAGCGCGATAGAAGAACTTTTCGCCCTGGCCGGCAAACAACTGAAAGAGTATGTGGAGTTGCTGCCCGTAACCCCTTTCTATCGGTTGTGCTGGGAATCCGGTAAGGTGTTCAATTATGACAACGATCAAACCCGTCTGGAAGCCCAGATTCAACAGTTTAACCCGCGTGACGTTGAAGGCTATCGGCAGTTCCTTGACTACAGTCGCGCTGTCTTTAAAGAGGGTTACTTAAAACTGGGCACCGTGCCGTTCTTGTCGTTCCGCGATATGCTACGCGCAGCGCCTCAATTAGCCAAGCTGCAGGCATGGCGTTCCGTGTATAGCAAAGTCGCGAGTTATATTGAGGATGAACACCTGCGCCAGGCCTTTTCTTTTCATAGTCTGTTGGTAGGTGGCAACCCGTTCGCCACGTCCTCAATCTACACCCTGATCCATGCGCTGGAGCGTGAATGGGGCGTGTGGTTCCCGCGCGGGGGCACGGGGGCCCTGGTGCAGGGCATGATCAAACTGTTCCAGGACCTGGGCGGAGAAGTGGTTTTGAATGCCCGCGTGTCGCATATGGAAACGACAGGCAATAAAATTGAAGCGGTGCATTTAGAAGACGGCCGTCGCTTTCTGACCCAGGCTGTTGCCTCGAACGCGGATGTGGTACACACCTATCGGGATCTGCTCAGCCAGCATCCGGCAGCCGTAAAACAGTCTAACAAACTGCAAACGAAACGTATGAGCAACTCACTGTTCGTTCTATATTTTGGCCTGAACCACCACCACGACCAACTGGCACATCACACGGTTTGTTTTGGGCCGCGGTATCGCGAATTAATTGATGAAATTTTTAATCATGACGGGTTGGCGGAAGATTTTTCCCTGTATTTGCACGCGCCGTGTGTGACCGATAGCAGTTTGGCCCCGGAAGGTTGCGGGAGCTACTATGTACTGGCGCCGGTGCCTCATCTTGGAACCGCAAACCTGGACTGGACCGTGGAAGGCCCAAAACTCCGCGACCGTATATTTGCGTATCTGGAACAACACTACATGCCCGGCCTCCGCAGTCAACTGGTGACTCACCGCATGTTTACCCCGTTTGATTTTCGTGATCAGCTGAATGCTTACCATGGAAGTGCATTTAGCGTGGAACCAGTACTGACACAATCCGCCTGGTTCCGCCCGCATAATAGGGATAAAACCATAACCAATCTCTATCTGGTGGGTGCTGGCACTCATCCGGGCGCTGGTATTCCGGGCGTAATCGGTAGCGCCAAAGCTACCGCAGGGTTGATGCTTGAGGATCTGATTTACGCCTGGAAAACATTACTGACCCCC + + + 1 + + + P21685_20000_rbs_seq + TCCGGATTCGTCACGCTCGTTCGTGCTGGTAGACTTTTATAACAGGAAGGAGGAAGACAC + + + 1 + + + P21685_30000_cds_seq + ATGAAACCGACTACGGTGATTGGCGCAGGTTTTGGCGGCTTGGCGCTTGCGATTCGGTTACAGGCCGCGGGCATCCCGGTACTGTTGCTCGAACAACGTGATAAACCTGGTGGGCGAGCGTACGTTTACGAGGATCAAGGCTTTACTTTTGATGCCGGTCCTACAGTAATTACTGATCCGAGCGCAATTGAAGAATTATTTGCCCTTGCAGGCAAACAGCTGAAAGAATATGTCGAGCTTCTCCCGGTGACGCCGTTTTATCGCCTGTGTTGGGAGAGCGGCAAAGTCTTCAACTACGACAATGATCAGACCCGTCTGGAAGCTCAGATTCAGCAGTTTAACCCGAGGGATGTGGAGGGCTACCGACAGTTTCTGGATTACAGTCGCGCCGTGTTTAAAGAAGGCTACCTGAAACTGGGGACTGTCCCTTTCCTGTCGTTCCGTGATATGCTGCGCGCGGCGCCTCAGTTAGCTAAGCTGCAAGCGTGGCGCTCGGTTTATTCTAAAGTAGCATCATATATCGAAGATGAACATCTGCGCCAGGCGTTTTCTTTTCATTCCCTGCTGGTCGGGGGTAATCCGTTTGCAACCTCCTCGATTTACACCCTGATCCATGCGTTAGAACGCGAATGGGGCGTATGGTTCCCACGCGGCGGCACAGGTGCCCTCGTACAGGGTATGATCAAACTCTTTCAGGATCTGGGCGGTGAAGTTGTGCTGAATGCGAGGGTATCACACATGGAAACGACCGGTAACAAAATCGAAGCCGTCCACCTGGAGGATGGACGCCGTTTTCTCACTCAGGCTGTGGCCAGTAACGCGGACGTTGTTCATACGTATCGTGATTTACTTAGCCAGCACCCGGCGGCAGTGAAACAGAGCAACAAATTGCAGACCAAGCGCATGTCTAACTCCCTGTTTGTGCTGTATTTTGGGCTTAACCACCACCACGATCAATTAGCGCACCACACCGTTTGCTTCGGCCCGCGCTACCGCGAGCTGATCGACGAGATTTTTAACCACGATGGATTAGCGGAAGACTTCTCTTTATATCTACATGCCCCGTGTGTGACCGACTCATCGCTCGCCCCGGAGGGTTGCGGTAGTTATTACGTTCTGGCGCCGGTGCCACACCTGGGCACCGCGAATTTGGACTGGACCGTGGAAGGTCCGAAGCTGCGCGATCGTATCTTCGCGTACCTGGAACAGCATTACATGCCAGGCCTGCGCTCCCAATTAGTTACCCATCGTATGTTCACGCCTTTTGATTTTCGCGACCAATTGAACGCCTACCATGGTTCGGCATTCAGCGTAGAACCGGTTCTGACGCAGTCGGCGTGGTTCCGCCCGCATAACCGTGATAAAACCATTACAAACCTGTACCTGGTGGGAGCAGGTACACATCCTGGTGCCGGCATTCCAGGTGTGATTGGTAGCGCGAAAGCTACCGCAGGCCTCATGTTAGAAGATCTGATT + + + 1 + + + P21685_30000_gene_seq + GGGACATGGGGATCGATCACCAATAGCTTAGCTAGATCGGTAAAATTTCCGCTGGGATACGGAGGGGAATGAAGTGAGGGGGAATAAATGAAACCGACTACGGTGATTGGCGCAGGTTTTGGCGGCTTGGCGCTTGCGATTCGGTTACAGGCCGCGGGCATCCCGGTACTGTTGCTCGAACAACGTGATAAACCTGGTGGGCGAGCGTACGTTTACGAGGATCAAGGCTTTACTTTTGATGCCGGTCCTACAGTAATTACTGATCCGAGCGCAATTGAAGAATTATTTGCCCTTGCAGGCAAACAGCTGAAAGAATATGTCGAGCTTCTCCCGGTGACGCCGTTTTATCGCCTGTGTTGGGAGAGCGGCAAAGTCTTCAACTACGACAATGATCAGACCCGTCTGGAAGCTCAGATTCAGCAGTTTAACCCGAGGGATGTGGAGGGCTACCGACAGTTTCTGGATTACAGTCGCGCCGTGTTTAAAGAAGGCTACCTGAAACTGGGGACTGTCCCTTTCCTGTCGTTCCGTGATATGCTGCGCGCGGCGCCTCAGTTAGCTAAGCTGCAAGCGTGGCGCTCGGTTTATTCTAAAGTAGCATCATATATCGAAGATGAACATCTGCGCCAGGCGTTTTCTTTTCATTCCCTGCTGGTCGGGGGTAATCCGTTTGCAACCTCCTCGATTTACACCCTGATCCATGCGTTAGAACGCGAATGGGGCGTATGGTTCCCACGCGGCGGCACAGGTGCCCTCGTACAGGGTATGATCAAACTCTTTCAGGATCTGGGCGGTGAAGTTGTGCTGAATGCGAGGGTATCACACATGGAAACGACCGGTAACAAAATCGAAGCCGTCCACCTGGAGGATGGACGCCGTTTTCTCACTCAGGCTGTGGCCAGTAACGCGGACGTTGTTCATACGTATCGTGATTTACTTAGCCAGCACCCGGCGGCAGTGAAACAGAGCAACAAATTGCAGACCAAGCGCATGTCTAACTCCCTGTTTGTGCTGTATTTTGGGCTTAACCACCACCACGATCAATTAGCGCACCACACCGTTTGCTTCGGCCCGCGCTACCGCGAGCTGATCGACGAGATTTTTAACCACGATGGATTAGCGGAAGACTTCTCTTTATATCTACATGCCCCGTGTGTGACCGACTCATCGCTCGCCCCGGAGGGTTGCGGTAGTTATTACGTTCTGGCGCCGGTGCCACACCTGGGCACCGCGAATTTGGACTGGACCGTGGAAGGTCCGAAGCTGCGCGATCGTATCTTCGCGTACCTGGAACAGCATTACATGCCAGGCCTGCGCTCCCAATTAGTTACCCATCGTATGTTCACGCCTTTTGATTTTCGCGACCAATTGAACGCCTACCATGGTTCGGCATTCAGCGTAGAACCGGTTCTGACGCAGTCGGCGTGGTTCCGCCCGCATAACCGTGATAAAACCATTACAAACCTGTACCTGGTGGGAGCAGGTACACATCCTGGTGCCGGCATTCCAGGTGTGATTGGTAGCGCGAAAGCTACCGCAGGCCTCATGTTAGAAGATCTGATTTACGCCTGGAAAACATTACTGACCCCC + + + 1 + + + P21685_30000_rbs_seq + TTAGCTAGATCGGTAAAATTTCCGCTGGGATACGGAGGGGAATGAAGTGAGGGGGAATAA + + + 1 + + + P21687_10000_cds_seq + ATGCAGCCGCACTATGATCTGATTCTGGTTGGAGCTGGTCTGGCGAACGGCCTAATAGCGCTGCGCCTCCAGCAACAGCAGCCGGACATGCGTATTCTTCTTATTGACGCCGCACCGCAGGCGGGTGGGAATCACACCTGGTCATTCCACCACGATGATCTGACCGAAAGTCAACATCGGTGGATCGCTCCGCTTGTTGTGCATCACTGGCCGGATTATCAGGTGCGTTTTCCGACCCGCCGTCGGAAACTGAATTCTGGATATTTCTGTATTACTAGTCAGCGTTTCGCCGAAGTCTTACAGCGTCAGTTCGGCCCACATCTGTGGATGGATACGGCCGTCGCGGAAGTCAATGCGGAATCGGTACGCCTGAAGAAGGGTCAGGTAATCGGAGCACGAGCGGTAATCGATGGGCGGGGCTATGCGGCAAACTCAGCTTTATCAGTTGGATTTCAAGCGTTTATTGGCCAAGAATGGCGTTTGAGTCACCCGCACGGCCTGAGCTCACCTATTATTATGGACGCAACTGTTGACCAGCAGAACGGTTACCGCTTTGTGTACTCACTGCCACTCTCACCTACCCGCCTCCTGATTGAAGATACTCATTATATTGACAATGCCACTTTGGACCCCGAGTGCGCGCGTCAGAATATCTGCGATTATGCAGCACAGCAAGGCTGGCAATTACAGACTCTGCTGCGCGAGGAACAAGGCGCGCTTCCGATTACCCTTAGTGGTAACGCGGATGCTTTCTGGCAGCAACGCCCGCTGGCGTGTTCAGGTCTGCGCGCGGGCCTGTTCCACCCGACAACCGGATATTCTCTGCCACTGGCAGTTGCGGTGGCGGATCGCCTCAGCGCTTTAGATGTGTTTACGTCAGCCAGCATACATCACGCAATTACCCACTTCGCGCGTGAAAGGTGGCAGCAACAGGGATTCTTCCGCATGTTAAATCGTATGCTATTTCTAGCCGGCCCAGCGGATTCAAGATGGCGTGTGATGCAGCGGTTCTATGGTCTGCCCGAGGACCTCATCGCGCGCTTCTATGCCGGTAAACTCACCCTGACTGATCGCTTACGCATTTTATCGGGGAAACCTCCCGTTCCGGTGCTAGCCGCACTTCAGGCGATTATGACGACCCACCGT + + + 1 + + + P21687_10000_gene_seq + GGGACATGGGGATCGATCACCAATAGCGCGACAAACGAACAACTCAGACACTTCGGTGATTGACTGTTATCTTGAAGGGGGCCTACAATGCAGCCGCACTATGATCTGATTCTGGTTGGAGCTGGTCTGGCGAACGGCCTAATAGCGCTGCGCCTCCAGCAACAGCAGCCGGACATGCGTATTCTTCTTATTGACGCCGCACCGCAGGCGGGTGGGAATCACACCTGGTCATTCCACCACGATGATCTGACCGAAAGTCAACATCGGTGGATCGCTCCGCTTGTTGTGCATCACTGGCCGGATTATCAGGTGCGTTTTCCGACCCGCCGTCGGAAACTGAATTCTGGATATTTCTGTATTACTAGTCAGCGTTTCGCCGAAGTCTTACAGCGTCAGTTCGGCCCACATCTGTGGATGGATACGGCCGTCGCGGAAGTCAATGCGGAATCGGTACGCCTGAAGAAGGGTCAGGTAATCGGAGCACGAGCGGTAATCGATGGGCGGGGCTATGCGGCAAACTCAGCTTTATCAGTTGGATTTCAAGCGTTTATTGGCCAAGAATGGCGTTTGAGTCACCCGCACGGCCTGAGCTCACCTATTATTATGGACGCAACTGTTGACCAGCAGAACGGTTACCGCTTTGTGTACTCACTGCCACTCTCACCTACCCGCCTCCTGATTGAAGATACTCATTATATTGACAATGCCACTTTGGACCCCGAGTGCGCGCGTCAGAATATCTGCGATTATGCAGCACAGCAAGGCTGGCAATTACAGACTCTGCTGCGCGAGGAACAAGGCGCGCTTCCGATTACCCTTAGTGGTAACGCGGATGCTTTCTGGCAGCAACGCCCGCTGGCGTGTTCAGGTCTGCGCGCGGGCCTGTTCCACCCGACAACCGGATATTCTCTGCCACTGGCAGTTGCGGTGGCGGATCGCCTCAGCGCTTTAGATGTGTTTACGTCAGCCAGCATACATCACGCAATTACCCACTTCGCGCGTGAAAGGTGGCAGCAACAGGGATTCTTCCGCATGTTAAATCGTATGCTATTTCTAGCCGGCCCAGCGGATTCAAGATGGCGTGTGATGCAGCGGTTCTATGGTCTGCCCGAGGACCTCATCGCGCGCTTCTATGCCGGTAAACTCACCCTGACTGATCGCTTACGCATTTTATCGGGGAAACCTCCCGTTCCGGTGCTAGCCGCACTTCAGGCGATTATGACGACCCACCGTTACGCCTGGAAAACATTACTGACCCCC + + + 1 + + + P21687_10000_rbs_seq + GCGACAAACGAACAACTCAGACACTTCGGTGATTGACTGTTATCTTGAAGGGGGCCTACA + + + 1 + + + P21687_20000_cds_seq + ATGCAGCCGCATTACGACTTGATTTTGGTGGGCGCCGGCCTGGCAAACGGCCTCATTGCCTTACGCTTACAGCAGCAGCAACCGGATATGCGCATCCTGCTCATTGATGCGGCCCCGCAGGCAGGTGGTAATCATACCTGGAGCTTTCATCATGATGATTTGACCGAATCGCAGCATCGTTGGATTGCGCCGTTAGTTGTTCATCACTGGCCAGACTATCAGGTGCGCTTCCCCACCCGACGCCGTAAACTGAACTCTGGCTATTTTTGCATTACCTCCCAGCGCTTTGCAGAAGTGCTGCAGCGCCAATTCGGCCCCCATTTGTGGATGGATACCGCCGTAGCCGAAGTAAATGCCGAATCCGTGCGGCTGAAGAAAGGTCAGGTCATTGGGGCTCGCGCGGTGATTGATGGGCGCGGATATGCGGCCAACAGTGCGCTGTCAGTGGGCTTTCAGGCCTTTATTGGCCAGGAATGGCGCCTTAGCCATCCCCACGGTCTCAGTTCCCCGATCATTATGGACGCCACTGTGGACCAACAAAACGGCTACCGTTTCGTGTACTCGCTCCCGCTTAGCCCGACCCGACTCTTGATCGAAGATACGCACTATATCGACAACGCCACCCTGGATCCGGAGTGTGCTCGTCAGAATATCTGTGACTACGCAGCGCAACAGGGTTGGCAGCTCCAAACCCTGCTACGCGAAGAACAGGGCGCACTGCCTATTACACTGTCCGGTAACGCGGACGCCTTTTGGCAGCAGCGCCCTCTGGCGTGTAGTGGCCTGCGTGCGGGCCTGTTTCATCCCACGACTGGATATAGTTTGCCGCTCGCGGTTGCGGTGGCGGATCGTCTCAGCGCACTTGATGTGTTTACTAGCGCAAGTATTCACCACGCGATTACCCATTTTGCGCGCGAACGTTGGCAGCAGCAAGGCTTCTTTCGTATGCTCAACCGCATGTTGTTCTTGGCGGGTCCAGCAGATTCGCGCTGGCGTGTGATGCAGCGTTTTTACGGGCTTCCGGAGGATCTCATTGCTCGCTTCTATGCGGGGAAACTGACCTTAACCGACCGACTGCGCATCCTGTCAGGAAAACCGCCTGTGCCGGTCTTAGCAGCGCTGCAAGCTATTATGACTACCCATCGC + + + 1 + + + P21687_20000_gene_seq + GGGACATGGGGATCGATCACCAATAGCCCACCCAATGGTAACCCAAAAATTCTCGATCCTCACGGATACGGAAGCAAAGGAGGTATCATGCAGCCGCATTACGACTTGATTTTGGTGGGCGCCGGCCTGGCAAACGGCCTCATTGCCTTACGCTTACAGCAGCAGCAACCGGATATGCGCATCCTGCTCATTGATGCGGCCCCGCAGGCAGGTGGTAATCATACCTGGAGCTTTCATCATGATGATTTGACCGAATCGCAGCATCGTTGGATTGCGCCGTTAGTTGTTCATCACTGGCCAGACTATCAGGTGCGCTTCCCCACCCGACGCCGTAAACTGAACTCTGGCTATTTTTGCATTACCTCCCAGCGCTTTGCAGAAGTGCTGCAGCGCCAATTCGGCCCCCATTTGTGGATGGATACCGCCGTAGCCGAAGTAAATGCCGAATCCGTGCGGCTGAAGAAAGGTCAGGTCATTGGGGCTCGCGCGGTGATTGATGGGCGCGGATATGCGGCCAACAGTGCGCTGTCAGTGGGCTTTCAGGCCTTTATTGGCCAGGAATGGCGCCTTAGCCATCCCCACGGTCTCAGTTCCCCGATCATTATGGACGCCACTGTGGACCAACAAAACGGCTACCGTTTCGTGTACTCGCTCCCGCTTAGCCCGACCCGACTCTTGATCGAAGATACGCACTATATCGACAACGCCACCCTGGATCCGGAGTGTGCTCGTCAGAATATCTGTGACTACGCAGCGCAACAGGGTTGGCAGCTCCAAACCCTGCTACGCGAAGAACAGGGCGCACTGCCTATTACACTGTCCGGTAACGCGGACGCCTTTTGGCAGCAGCGCCCTCTGGCGTGTAGTGGCCTGCGTGCGGGCCTGTTTCATCCCACGACTGGATATAGTTTGCCGCTCGCGGTTGCGGTGGCGGATCGTCTCAGCGCACTTGATGTGTTTACTAGCGCAAGTATTCACCACGCGATTACCCATTTTGCGCGCGAACGTTGGCAGCAGCAAGGCTTCTTTCGTATGCTCAACCGCATGTTGTTCTTGGCGGGTCCAGCAGATTCGCGCTGGCGTGTGATGCAGCGTTTTTACGGGCTTCCGGAGGATCTCATTGCTCGCTTCTATGCGGGGAAACTGACCTTAACCGACCGACTGCGCATCCTGTCAGGAAAACCGCCTGTGCCGGTCTTAGCAGCGCTGCAAGCTATTATGACTACCCATCGCTACGCCTGGAAAACATTACTGACCCCC + + + 1 + + + P21687_20000_rbs_seq + CCACCCAATGGTAACCCAAAAATTCTCGATCCTCACGGATACGGAAGCAAAGGAGGTATC + + + 1 + + + P21687_30000_cds_seq + ATGCAGCCTCATTACGATTTGATTCTTGTCGGTGCAGGTCTGGCGAACGGCTTAATTGCACTGCGCCTGCAGCAGCAGCAACCGGACATGAGGATTCTTCTTATTGATGCCGCGCCGCAAGCGGGTGGAAATCATACTTGGAGCTTTCACCACGATGACCTGACCGAATCGCAACATCGTTGGATTGCTCCGTTGGTGGTGCATCACTGGCCGGATTATCAAGTTCGCTTTCCCACCCGTCGTCGTAAATTGAACTCCGGTTACTTTTGTATCACGAGTCAACGTTTTGCTGAAGTCTTGCAGCGTCAATTCGGGCCGCATCTGTGGATGGATACTGCAGTGGCGGAAGTTAATGCCGAGTCAGTGCGGCTGAAGAAGGGCCAGGTCATCGGAGCACGTGCGGTCATTGATGGTCGCGGATATGCGGCGAATTCCGCACTGAGCGTAGGCTTCCAGGCGTTCATTGGCCAGGAATGGCGTCTGAGCCATCCCCACGGCTTAAGCAGCCCGATTATCATGGACGCGACCGTAGATCAGCAAAACGGTTACCGTTTCGTGTACTCATTGCCTTTATCCCCGACCCGTCTGTTAATTGAGGACACCCACTACATCGATAATGCGACCCTGGATCCGGAGTGTGCGCGTCAGAACATTTGTGACTACGCGGCCCAGCAGGGATGGCAGCTGCAAACGTTACTGCGTGAAGAACAAGGTGCGTTGCCGATCACACTTAGCGGCAATGCGGATGCGTTTTGGCAGCAGAGACCGCTGGCTTGTAGCGGTCTGCGCGCAGGTTTATTTCACCCCACCACGGGATATTCTCTCCCGTTGGCGGTAGCGGTTGCCGATAGGCTGAGCGCGTTAGACGTTTTTACGAGTGCGTCGATTCACCATGCGATTACTCACTTCGCCCGTGAACGCTGGCAGCAGCAGGGCTTCTTCCGCATGCTCAATCGGATGCTGTTTCTCGCTGGTCCGGCAGATTCGCGCTGGCGTGTGATGCAACGCTTTTACGGCTTACCTGAGGATTTAATTGCTCGTTTCTATGCTGGCAAACTAACTTTAACGGATCGCTTACGCATTCTGTCGGGCAAACCGCCCGTTCCGGTGCTTGCCGCCCTGCAAGCAATCATGACCACCCATCGC 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GGGACATGGGGATCGATCACCAATAGCCTCCTGAATTCTAAGTCCTTTCCTGAATATGCCTACATAACGCTTAGAGGGAGGTAGAAAATGCAGCCTCATTACGATTTGATTCTTGTCGGTGCAGGTCTGGCGAACGGCTTAATTGCACTGCGCCTGCAGCAGCAGCAACCGGACATGAGGATTCTTCTTATTGATGCCGCGCCGCAAGCGGGTGGAAATCATACTTGGAGCTTTCACCACGATGACCTGACCGAATCGCAACATCGTTGGATTGCTCCGTTGGTGGTGCATCACTGGCCGGATTATCAAGTTCGCTTTCCCACCCGTCGTCGTAAATTGAACTCCGGTTACTTTTGTATCACGAGTCAACGTTTTGCTGAAGTCTTGCAGCGTCAATTCGGGCCGCATCTGTGGATGGATACTGCAGTGGCGGAAGTTAATGCCGAGTCAGTGCGGCTGAAGAAGGGCCAGGTCATCGGAGCACGTGCGGTCATTGATGGTCGCGGATATGCGGCGAATTCCGCACTGAGCGTAGGCTTCCAGGCGTTCATTGGCCAGGAATGGCGTCTGAGCCATCCCCACGGCTTAAGCAGCCCGATTATCATGGACGCGACCGTAGATCAGCAAAACGGTTACCGTTTCGTGTACTCATTGCCTTTATCCCCGACCCGTCTGTTAATTGAGGACACCCACTACATCGATAATGCGACCCTGGATCCGGAGTGTGCGCGTCAGAACATTTGTGACTACGCGGCCCAGCAGGGATGGCAGCTGCAAACGTTACTGCGTGAAGAACAAGGTGCGTTGCCGATCACACTTAGCGGCAATGCGGATGCGTTTTGGCAGCAGAGACCGCTGGCTTGTAGCGGTCTGCGCGCAGGTTTATTTCACCCCACCACGGGATATTCTCTCCCGTTGGCGGTAGCGGTTGCCGATAGGCTGAGCGCGTTAGACGTTTTTACGAGTGCGTCGATTCACCATGCGATTACTCACTTCGCCCGTGAACGCTGGCAGCAGCAGGGCTTCTTCCGCATGCTCAATCGGATGCTGTTTCTCGCTGGTCCGGCAGATTCGCGCTGGCGTGTGATGCAACGCTTTTACGGCTTACCTGAGGATTTAATTGCTCGTTTCTATGCTGGCAAACTAACTTTAACGGATCGCTTACGCATTCTGTCGGGCAAACCGCCCGTTCCGGTGCTTGCCGCCCTGCAAGCAATCATGACCACCCATCGCTACGCCTGGAAAACATTACTGACCCCC 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+ + 10 + 1 + + + + + + + 1 + + + + + PolyA + annotation1898430 + + + range1898430 + 39 + + + 32 + 1 + + + + + + + 1 + + + + + PolyA + annotation1898431 + + + range1898431 + 9 + + + 1 + 1 + + + + + + + 1 + + + + 1 + false + true + + _41_ + 0 + 745 + 41 + Released HQ 2013 + In stock + + false + Artificial terminator, estimated %T~>90% +*8bp stem, 6nt loop +*Bidirectional, estimated reverse %T~>90% + Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. + modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs + true + + + + + BBa_B1006_sequence + aaaaaaaaaccccgcccctgacagggcggggtttttttt + + + 1 + + + + + robertb + 2004-07-18T11:00:00Z + https://synbiohub.org/public/igem/BBa_I13800/1 + 2015-08-31T04:07:36Z + res1 + BBa_I13800 + + + + + + + Ampicillin Resistance + annotation902300 + + + range902300 + 861 + + + 1 + 1 + + + + + + + 1 + + + + 1 + false + false + + _6_ + 0 + 159 + 7 + Not in stock + + false + This is the wild-type cassette for ampicillin resistance. + false + + + + + BBa_I13800_sequence + ttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcat + + + 1 + + + + + Reshma Shetty + 2007-04-10T11:00:00Z + https://synbiohub.org/public/igem/BBa_I50032/1 + 2015-08-31T04:07:42Z + p15A + BBa_I50032 + + + + + + + p15A origin of replication + annotation1928307 + + + range1928307 + 1223 + + + 399 + 1 + + + + + + + 1 + + + + + repeat region + annotation1928308 + + + range1928308 + 1450 + + + 1223 + 1 + + + + + + + 1 + + + + 1 + false + false + + _41_ + 0 + 126 + 70 + Not in stock + + false + p15A origin of replication which is supposed to have low to medium copy number. It is also the origin of replication in pSB3* series plasmids. + The boundaries of this part are quite liberal to ensure that this part is a functional origin. + This part was constructed by PCR amplification of pSB3K3-P1010. + false + + + + + BBa_I50032_sequence + atggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtt + + + 1 + + + + + Reshma Shetty + 2006-03-13T12:00:00Z + https://synbiohub.org/public/igem/BBa_I50041/1 + 2015-08-31T04:07:42Z + BBR1 + BBa_I50041 + + + + + + + putative polyA + annotation1932215 + + + range1932215 + 91 + + + 76 + 1 + + + + + + + 1 + + + + + repA + annotation1932216 + + + range1932216 + 1152 + + + 202 + 1 + + + + + + + 1 + + + + + inverted repeat + annotation1932217 + + + range1932217 + 1176 + + + 1154 + 1 + + + + + + + 1 + + + + + RBS + annotation1932218 + + + range1932218 + 1159 + + + 1154 + 1 + + + + + + + 1 + + + + + RBS + annotation1932219 + + + range1932219 + 1168 + + + 1156 + 1 + + + + + + + 1 + + + + + promoter region + annotation1932220 + + + range1932220 + 1192 + + + 1173 + 1 + + + + + + + 1 + + + + + inverted repeat + annotation1932221 + + + range1932221 + 1200 + + + 1179 + 1 + + + + + + + 1 + + + + + rep promoter -10 + annotation1932222 + + + range1932222 + 1188 + + + 1183 + 1 + + + + + + + 1 + + + + + rep promoter -35 + annotation1932223 + + + range1932223 + 1210 + + + 1205 + 1 + + + + + + + 1 + + + + + Mutated SpeI site + annotation1932224 + + + range1932224 + 1224 + + + 1219 + 1 + + + + + + + 1 + + + + + direct repeat + annotation1932225 + + + range1932225 + 1269 + + + 1250 + 1 + + + + + + + 1 + + + + + direct repeat + annotation1932226 + + + range1932226 + 1301 + + + 1282 + 1 + + + + + + + 1 + + + + + direct repeat + annotation1932227 + + + range1932227 + 1322 + + + 1303 + 1 + + + + + + + 1 + + + + + AT rich region + annotation1932228 + + + range1932228 + 1403 + + + 1321 + 1 + + + + + + + 1 + + + + + par region + annotation1932229 + + + range1932229 + 1901 + + + 1534 + 1 + + + + + + + 1 + + + + + inverted repeat + annotation1932230 + + + range1932230 + 1817 + + + 1800 + 1 + + + + + + + 1 + + + + + inverted repeat + annotation1932231 + + + range1932231 + 1846 + + + 1823 + 1 + + + + + + + 1 + + + + + inverted repeat + annotation1932232 + + + range1932232 + 1873 + + + 1857 + 1 + + + + + + + 1 + + + + + Tn3 integration site + annotation1932233 + + + range1932233 + 1880 + + + 1876 + 1 + + + + + + + 1 + + + + + inverted repeat + annotation1932234 + + + range1932234 + 1899 + + + 1886 + 1 + + + + + + + 1 + + + + + terminator + annotation1932235 + + + range1932235 + 1992 + + + 1963 + 1 + + + + + + + 1 + + + + + difference from canonical pSC101 sequence in Genbank + annotation2080856 + + + range2080856 + 36 + + + 36 + 1 + + + + + + + 1 + + + + + difference from canonical pSC101 sequence in Genbank + annotation2080857 + + + range2080857 + 126 + + + 126 + 1 + + + + + + + 1 + + + + + difference from canonical pSC101 sequence in Genbank + annotation2080858 + + + range2080858 + 288 + + + 288 + 1 + + + + + + + 1 + + + + + difference from canonical pSC101 sequence in Genbank + annotation2080859 + + + range2080859 + 383 + + + 383 + 1 + + + + + + + 1 + + + + 1 + false + false + + _41_6_ + 0 + 126 + 45 + Not in stock + + false + pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids. This part is the reverse copy of BBa_I50040. + false + + + + + BBa_I50041_sequence + gttacattgtcgatctgttcatggtgaacagctttgaatgcaccaaaaactcgtaaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttatgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacag + + + 1 + + + + + Matt Eames + 2006-10-31T12:00:00Z + https://synbiohub.org/public/igem/BBa_J56012/1 + 2015-08-31T03:22:59Z + Ptrc + BBa_J56012 + + + + + 1 + false + false + _79_98_ + 0 + 1132 + 98 + Not in stock + + false + Seuqence is inverted by the FimE protein. Inside recognition sequence is a Ptrc promoter sequence. Switch is irreversible. + None + Synthesized by DNA 2.0 + false + + + + + BBa_J56012_sequence + ctgccaacactgcacagttttcccccaaaagatgaaacatttggggccaaactgtccatattatacgagccggatgattaattgtcaaccataaaaatattaaaaaacgacaaaaagcatctaactgtttgatatgtaaattatttctattgtaaattaatttcacatcacctccgctatatgtaaagctaacgtttctgtggctcgacgcatcttcctcattcttctctccaaaaaccacctcatgcaatataaacatctataaataaagataacaatagaatattaagccaacaaataaactgaaaaagtttgtccgcgatgctttcctctatgagtcaaaatggccccaattgtcttgtatttatttgtttttttaactttattatcaataagttaaatcggtacc + + + 1 + + + + + Jeffrey J Tabor + 2007-04-13T11:00:00Z + https://synbiohub.org/public/igem/BBa_J64101/1 + 2015-05-08T01:08:17Z + ColE1 + BBa_J64101 + + + + + + + BBa_J64100 + annotation1931320 + + + range1931320 + 2800 + + + 1 + 1 + + + + + + + 1 + + + + + G00100 + annotation1931321 + + + range1931321 + 25 + + + 6 + 1 + + + + + + + 1 + + + + + prefix + annotation1931322 + + + range1931322 + 145 + + + 124 + 1 + + + + + + + 1 + + + + + B0025 + annotation1931323 + + + range1931323 + 274 + + + 146 + 1 + + + + + + + 1 + + + + + suffix + annotation1931324 + + + range1931324 + 295 + + + 275 + 1 + + + + + + + 1 + + + + + G00101 + annotation1931325 + + + range1931325 + 450 + + + 431 + 1 + + + + + + + 1 + + + + + G00101 + annotation1931326 + + + range1931326 + 502 + + + 483 + 1 + + + + + + + 1 + + + + 1 + false + false + + _98_ + 0 + 88 + 98 + Not in stock + + false + ColE1 biobrick plasmid with backward terminator in MCS + None + Composite registry part derived from J64100 and B0025 + false + + + + + BBa_J64101_sequence + tcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatcctagggcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgacttgtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacagacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccagatatcgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcacc + + + 1 + + + + + Anna Fomitcheva, Lisa Baumgartner + 2015-08-25T11:00:00Z + https://synbiohub.org/public/igem/BBa_K1847014/1 + 2015-08-26T09:07:45Z + PlacUV5 + BBa_K1847014 + + + + + + plac UV5 + annotation2438101 + + + range2438101 + 31 + + + 1 + 1 + + + + + + + 1 + + + + + lacO + annotation2438102 + + + range2438102 + 55 + + + 39 + 1 + + + + + + + 1 + + + + 1 + false + false + + _2273_ + 26442 + 26442 + 9 + + false + Promoter regulated by LacI. + Synthetic synthesis. + false + + + + + BBa_K1847014_sequence + tttacactttatgcttccggctcgtataatgtgtggaattgtgagcggataacaa + + + 1 + + + + + Chris J. Myers + James Alastair McLaughlin + Conversion of the iGEM parts registry to SBOL2.1 + iGEM to SBOL conversion + igem2sbol + + 1 + 2017-03-06T15:00:00.000Z + +