# HG changeset patch # User tduigou # Date 1682904512 0 # Node ID 6f447c1584bf9e9a2d747f83ad98626e258eabe5 # Parent afbbecdcc0e3df3656c869013f017943d9eed8b6 planemo upload for repository https://github.com/neilswanston/LCRGenie commit 2e8aa2e7c0581c7eed2074d15f7e4317ecde2ad6 diff -r afbbecdcc0e3 -r 6f447c1584bf config/job_conf.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/config/job_conf.xml Mon May 01 01:28:32 2023 +0000 @@ -0,0 +1,1 @@ + diff -r afbbecdcc0e3 -r 6f447c1584bf config/tool_conf.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/config/tool_conf.xml Mon May 01 01:28:32 2023 +0000 @@ -0,0 +1,3 @@ +
+ +
diff -r afbbecdcc0e3 -r 6f447c1584bf lcrgenie.xml --- a/lcrgenie.xml Thu Feb 03 13:30:30 2022 +0000 +++ b/lcrgenie.xml Mon May 01 01:28:32 2023 +0000 @@ -1,7 +1,8 @@ - - Ligase Chain Reaction + + Web-based tool for supporting the design of bridging oligos 1.0.2 + 0 lcr_genie @@ -13,14 +14,14 @@ - +
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@@ -41,41 +42,34 @@ LCRGenie ======== -LCR Genie is a web-based tool for supporting the design of bridging oligos, which are required for annealing together individual synthetic DNA parts (designed by PartsGenie) into multi-gene plasmid assemblies, designed by OptDoE. +LCR Genie is a web-based tool for supporting the design of bridging oligos, which are required for annealing together individual synthetic DNA parts (designed by PartsGenie) into multi-gene plasmid assemblies, designed by OptDoE. + +Enzyme identifiers are randomly chosen and combined with the aforementioned parts to explore the combinatorics of possible constructs. + +LCR Genie takes as input an SBOL (Synthetic Biology Open Language) document containing numerous combinatorial plasmid assemblies, and designing bridging oligos necessary for assembly via the ligase cycling reaction method. The LCR Genie node performs analogous functionality to the `DNA weaver `_ node (supporting multi-part assembly but by a different experimental method) and as such, its output format matches that of DNA Weaver. Input ----- -* **input**\ : (string) is the path to an .xml SBOL file containing constructs designs and sequences -* **melting_temp**\ : (float) is the target melting temperature for the bridging oligos (default=60.0) +* **Constructs**\ : SBOL (Synthetic Biology Open Language) file containing constructs designs and sequences +* **Melting temperature**\ : (float) is the target melting temperature for the bridging oligos (default=60.0) Output ------ -* **output**\ : (string) is the path to the output spreadsheet - -Project Links ---------------------- - -* `GitHub `_ - -Version ----------- - -1.0.2 - -Authors -------- - -* **Neil Swainston** -* **Joan Hérisson** - -License -------- - -`MIT `_ - - +* **Assembly Plan**\ : Output spreadsheet ]]> + + + + + + @unpublished{LCRGenie + author = {Neil Swainston}, + title = {{LCRGenie}}, + url = {https://github.com/neilswanston/LCRGenie/}, + } + +