LOCUS       .                       1886 bp    DNA              UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     terminator      392..419
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(431..1018)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      1040..1069
                     /note="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     misc_feature    1143..1148
                     /note="BsmBI"
     source          5..1149
                     /source="Exported"
     misc_feature    1154..1165
                     /note="KozaK"
     CDS             1162..1164
                     /codon_start=1
                     /note="ATG"
                     /translation="M"
     CDS             1165..1224
                     /codon_start=1
                     /note="Ig-kappa leader"
                     /product="leader sequence from mouse immunoglobulin kappa
                     light chain"
                     /translation="ETDTLLLWVLLLWVPGSTGD"
     source          1154..1226
                     /source="Exported"
     misc_feature    complement(1232..1237)
                     /note="BsmBI"
     terminator      1304..1335
                     /note="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        1336..1438
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene"
     source          1231..1886
                     /source="Exported"
ORIGIN
        1 ctcaggcgca atcacgaatg aataacggtt tggttggtgc gagtgatttt gatgacgagc
       61 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac
      121 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
      181 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
      241 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
      301 aatatggtat tgataatcct gatatgaata aattgcagtt tcacttgatg ctcgatgagt
      361 ttttctaatg agggcccaaa tgtaatcacc tggctcacct tcgggtgggc ctttctgcgt
      421 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgatgctcaa
      481 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      541 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      601 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      661 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      721 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
      781 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      841 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      901 agccagttac ctcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      961 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1021 agatcctttg attttctacc gaagaaaggc ccacccgtga aggtgagcca gtgagttgat
     1081 tgcagtccag ttacgctgga gtctgaggct cgtcctgaat gatatcaagc ttgaattcgt
     1141 tacgtctcgc cagccgccac catggaaaca gacacactgc tgctatgggt actgctgctc
     1201 tgggttccag gttccactgg tgacagcagc cgagacgaag acgaattctc tagatatcgc
     1261 tcaatactga ccatttaaat catacctgac ctccatagca gaaagtcaaa agcctccgac
     1321 cggaggcttt tgacttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag
     1381 atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat
     1441 gagccatatt caacgggaaa cgtcttgctc gaggccgcga ttaaattcca acatggatgc
     1501 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     1561 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     1621 tgccaatgat gttacagatg agatggtcag gctaaactgg ctgacggaat ttatgcctct
     1681 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     1741 cccagggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     1801 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     1861 taacggcgat cgcgtatttc gtctcg
//
