colon biopsy, from cancer patient, normal

Genomic DNA was extracted and purified from frozen samples using Qiagen.

Bisulphite-converted DNA was amplified, fragmented and hybridised to Illumina HumanMethylation450 Beadchips using the standard Illumina protocol.

Arrays were imaged using iScan Reader using the standard recommended Illumina scanner setting.

GenomeStudio Software v2011.1. Average Beta values were normalized using internal controls.

Research Institute of Physico-Chemical Medicine