Mercurial > repos > thanhlv > biotradis
changeset 2:0cfaf8b3049f draft
Deleted selected files
author | thanhlv |
---|---|
date | Thu, 25 Jul 2019 06:08:17 -0400 |
parents | eb1e9759c24e |
children | d44c3626166e |
files | biotradis_bacteria_tradis.xml |
diffstat | 1 files changed, 0 insertions(+), 93 deletions(-) [+] |
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--- a/biotradis_bacteria_tradis.xml Thu Jul 25 06:07:06 2019 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,93 +0,0 @@ -<tool id="bacteria_tradis" name="bacteria tradis" version="@VERSION@"> - <description>Generates a BAM file with tags added to read strings.</description> - <macros> - <import>macros.xml</import> - </macros> - <expand macro="requirements" /> - <command detect_errors="exit_code"><![CDATA[ - #import re - - ## Creates symlinks for each input file based on the Galaxy 'element_identifier' - ## Used so that a human-readable name appears in the output table (instead of 'dataset_xyz.dat') - #set $named_input_files = '' - #for $input_file in $input_files - ## Add single quotes around each input file identifier - #set $_input_file = "'{}'".format($input_file.element_identifier) - ln -s '${input_file}' ${_input_file} && - echo ${_input_file} >> fq.list && - #end for - - bacteria_tradis -f fq.list - -t $tag - -r $ref - -td $tag_dir - -mm $mm - -m $m - #if str($smalt_k)!="" - --smalt_k $smalt_k - #end if - #if str($smalt_s)!="" - --smalt_s $smalt_s - #end if - --smalt_y $smalt_y - --smalt_r $smalt_r - -n "\${GALAXY_SLOTS:-2}" - #if $verbose - --verbose - #end if - && tar cvzf insert_site_plot.tar.gz *insert_site_plot.gz - && tar cvzf mapped.tar.gz *mapped.bam* - ]]></command> - <inputs> - <param name="input_files" type="data" format="fastqsanger,fastqsanger.gz,fastqsanger.bz2" multiple="true" /> - <param name="tag" type="text" label="Tag" help="Tag to search for" /> - <param name="ref" type="data" format="fasta" label="Reference geome" help="Fasta format" /> - <param name="tag_dir" type="select" label="Tag direction" help="3 or 5(optional. default = 3)" > - <option value="3" selected="true">3</option> - <option value="5">5</option> - </param> - <param name="mm" type="integer" value="0" label="Number of mismatches allowed when matching tag" help="(optional. default = 0)" /> - <param name="m" type="integer" value="30" label="Mapping quality cutoff score" help="(optional. default = 30)" /> - <param argument="--smalt_k" type="integer" value="" optional="True" label="Custom k-mer value for SMALT mapping" help="(optional)" /> - <param argument="--smalt_s" type="integer" value="" optional="True" label="Custom step size for SMALT mapping" help="(optional)" /> - <param argument="--smalt_y" type="integer" value="0.96" label="Custom y parameter for SMALT" help="(optional. default = 0.96)" /> - <param argument="--smalt_r" type="integer" value="-1" label="custom r parameter for SMALT" help="(optional. default = -1)" /> - <param argument="--verbose" type="boolean" truevalue="-v" falsevalue="" checked="false" label="verbose debugging output" help="(Default:No)" /> - </inputs> - - <outputs> - <data name="insert_size_plots" format="tar.gz" label="${tool.name} on ${on_string}: Insert size plot" from_work_dir="insert_site_plot.tar.gz"/> - <data name="mapped_bam" format="tar.gz" label="${tool.name} on ${on_string}: Insert size plot" from_work_dir="mapped.tar.gz"/> - <data name="fastq_stats" format="txt" label="${tool.name} on ${on_string}: Fastq Stats" from_work_dir="fastqs.stats"/> - </outputs> - - <help> - <![CDATA[ - Run a TraDIS analysis. This involves: - 1: filtering the data with tags matching that passed via -t option - 2: removing the tags from the sequences - 3: mapping - 4: creating an insertion site plot - 5: creating a stats summary - - Usage: bacteria_tradis [options] - - Options: - -f : text file listing fastq files with tradis tags attached - -t : tag to search for - -r : reference genome in fasta format (.fa) - -td : tag direction - 3 or 5 (optional. default = 3) - -mm : number of mismatches allowed when matching tag (optional. default = 0) - -m : mapping quality cutoff score (optional. default = 30) - --smalt_k : custom k-mer value for SMALT mapping (optional) - --smalt_s : custom step size for SMALT mapping (optional) - --smalt_y : custom y parameter for SMALT (optional. default = 0.96) - --smalt_r : custom r parameter for SMALT (optional. default = -1) - -n : number of threads to use for SMALT and samtools sort (optional. default = 1) - -e : set defaults for essentiality experiment (smalt_r = 0, -m = 0) - -v : verbose debugging output - - Documentation can be found at `site <https://github.com/sanger-pathogens/Bio-Tradis>`_. - ]]></help> - -</tool>