Mercurial > repos > tiagoantao > barcode_stacks
diff barcode_sort.xml @ 0:5dc02b8a2a57 draft default tip
planemo upload commit 70654da77972b29181d3b388035836b6fa84d59d-dirty
author | tiagoantao |
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date | Fri, 08 Apr 2016 11:38:00 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/barcode_sort.xml Fri Apr 08 11:38:00 2016 -0400 @@ -0,0 +1,52 @@ +<tool id="barcode_sort_stacks" name="Barcode sorting for Stacks" version="2.0.2" force_history_refresh="True"> + <description>Barcode Sorting with Paired-End reads for Stacks</description> + +<stdio> + <exit_code range="1" level="fatal" description="Error in script execution" /> +</stdio> + +<command interpreter="perl"> + +barcode_sort.pl $barcode $f1 $f2 $barcoded $nonbarcoded true $barcode_length $cut_site $max_errors + +</command> + +<inputs> + <param name="barcode" format="text" type="data" label="Barcode file" /> + <param name="barcode_length" type="integer" value="6" label="Barcode length" /> + <param name="f1" type="data" format="fastq" label="First read FASTQ" /> + <param name="f2" type="data" format="fastq" label="Second read FASTQ" /> + <param name="cut_site" type="text" value="TGCA" label="Cut site" /> + <param name="max_errors" type="integer" value="2" label="Maximum number of errors" /> +</inputs> +<outputs> + <data format="fastq" name="barcoded" label="Barcoded sequences"/> + <data format="fastq" name="nonbarcoded" label="Non barcoded sequences"/> +</outputs> + +<help> + +.. class:: infomark + +**What it does** + +This program takes 2 input sequence files where the barcode can be on either +the 1st or 2nd read (but not both) and sorts all barcoded reads into a single +file and all non-barcoded files into a second file. This separation is needed +for the STACKs program as it does not handle sequences where the barcode is +arbitrarily on the 1st or 2nd read. + +-------- + +**Created by:** + +Paul Hohenlohe with changes from Brian Hand. + +**Integrated by:** + +Tiago Antao + + +</help> + +</tool>