# HG changeset patch # User tyty # Date 1413785496 14400 # Node ID 52b0eb6bef3153f0b184004ed535b3532586381d # Parent 42b0b1e0e696f9f4e7a400a62ddbaffd197e7398 Deleted selected files diff -r 42b0b1e0e696 -r 52b0eb6bef31 get_reads/predict_RNAs.py --- a/get_reads/predict_RNAs.py Mon Oct 20 02:11:19 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,93 +0,0 @@ -#RNA structure prediction & Output and illustrate reactivities - -import sys -from parse_dis_pac import * -from read_file import * -from Bio import SeqIO -import os -from rtts_plot import * - - -id_file = sys.argv[1] -seq_file = sys.argv[2] -output_file = sys.argv[4] - - -flag = 0 -if sys.argv[3]!='None': #input reactivity file if provided - react_file = sys.argv[3] - react = parse_dist(react_file) - react = react[1] - flag = 1 - -ospath = os.path.realpath(sys.argv[0]) -ost = ospath.split('/') -syspath = "" -for i in range(len(ost)-1): - syspath = syspath+ost[i].strip() - syspath = syspath+'/' - -ids = read_t_file(id_file) -sequences = SeqIO.parse(seq_file, 'fasta') - - -seqs = {} -for seq in sequences: - seqs[seq.id] = seq.seq.tostring() - -if len(ids)>10: #setup a limit of the number of sequence to be predicted - print("Number of sequences exceeds limitation!") - sys.exit(0) - - -#predict RNA structures -os.system("mkdir "+syspath+"output_f") -for i in range(len(ids)): - id_s = ids[i][0] - print(id_s) - #Put RNA sequence and reactivities into files - if id_s in seqs: - f = file(syspath+"temp.txt", 'w') - f.write('>'+id_s) - f.write('\n') - f.write(seqs[id_s]) - f.close() - if flag == 0: - os.system("Fold "+syspath+"temp.txt"+" "+syspath+"output_f/"+id_s+".ct") - if flag == 1: - if id_s in react: - f = file(syspath+"constraint.txt",'w') - make_plot(react[id_s],id_s,(syspath+"output_f/")) #make a plot of the distribution of the reactivites of the input RNA - #h = file(syspath+"output_f/transcript_reactivities.txt", 'w') - #h.write(id_s) - #h.write('\n') - for j in range(0, (len(react[id_s]))): - if react[id_s][j]!='NA': - f.write(str(j+1)) - f.write('\t') - f.write(str(react[id_s][j])) - f.write('\n') - #h.write(str(react[id_s][j])) #Output the reactivities - #h.write('\t') - f.close() - #h.write('\n') - #h.write('\n') - os.system("Fold "+syspath+"temp.txt"+" -sh"+" "+syspath+"constraint.txt"+" "+syspath+"output_f/"+id_s+".ct") - else: - print(id_s+" not in the data of react!") - os.system("draw "+syspath+"output_f/"+id_s+".ct "+syspath+"output_f/"+id_s+".ps") - else: - print(id_s+" not in the data of sequences!") - -#Remove the unnecessary files -os.system("tar -zcvPf "+output_file+" "+syspath+"output_f/"+"*.* 2>"+syspath+"log.txt") -os.system("rm -f "+syspath+"temp.txt") -os.system("rm -r "+syspath+"output_f") -if flag == 1: - os.system("rm -f "+syspath+"constraint.txt") - # h.close() - - - - -