Mercurial > repos > tyty > structurefold
changeset 4:a292aaf51735 draft
Uploaded
author | tyty |
---|---|
date | Mon, 15 Sep 2014 14:52:43 -0400 |
parents | f4cc06e92530 |
children | 7a8ddf1819b1 |
files | Iterative_mapping/iterative_map.py Iterative_mapping/iterative_map.xml Iterative_mapping/map_ex.py Iterative_mapping/read_file.py Iterative_mapping/read_s_file.py Iterative_mapping/remove_map.py Iterative_mapping/seq_track.py Iterative_mapping/tool_dependencies.xml Iterative_mapping/truncate.py Iterative_mapping/unmap.py predict/.DS_Store predict/parse_dis_pac.py predict/predict_RNAs.py predict/predict_RNAs.xml predict/rRNA.txt predict/rRNA_react.txt predict/read_file.py predict/rtts_plot.py predict/tool_dependencies.xml |
diffstat | 19 files changed, 301 insertions(+), 402 deletions(-) [+] |
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--- a/Iterative_mapping/iterative_map.py Mon Sep 15 14:52:20 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,103 +0,0 @@ -#!/usr/bin/env python -# -*- coding: utf-8 -*- - -import sys -import os -from read_file import * -from read_s_file import * - -type_input = sys.argv[1] -seq_file = sys.argv[2] -ref_file = sys.argv[3] -shift = sys.argv[4] -length = sys.argv[5] -map_type = sys.argv[6] -output_file = sys.argv[7] - - -if map_type!="default": - s = "" - s = s+"-v "+sys.argv[8] - s = s+" -5 "+sys.argv[9] - s = s+" -3 "+sys.argv[10] - s = s+" -k "+sys.argv[11] - if sys.argv[12]: - s = s+" -a" - if int(sys.argv[13])>=1: - s = s+" -m "+sys.argv[13] - if sys.argv[14]: - s = s+" --best --strata " - -else: - s = "-v 3 -a --best --strata " - -ospath = os.path.realpath(sys.argv[0]) -ost = ospath.split('/') -syspath = "" -for i in range(len(ost)-1): - syspath = syspath+ost[i].strip() - syspath = syspath+'/' - -os.system("bowtie-build -f "+ref_file+" "+syspath+"ref > "+syspath+"log.txt") - -os.system("cp "+seq_file+" "+syspath+"seq0.fa") - -if type_input == "fasta": - tp = 'fasta' -if type_input == "fastq": - tp = 'fastq' - -k = 0 -print(type_input) -while(True): - if type_input == "fasta": - os.system("bowtie "+s+"-f "+syspath+"ref"+" "+syspath+"seq"+str(k)+".fa --quiet -S > "+syspath+"map"+str(k)+".sam") - if type_input == "fastq": - os.system("bowtie "+s+"-q "+syspath+"ref"+" "+syspath+"seq"+str(k)+".fa --quiet -S > "+syspath+"map"+str(k)+".sam") - os.system("samtools view -Sb -F 0xfff "+syspath+"map"+str(k)+".sam > "+syspath+"mapped"+str(k)+".bam 2>"+syspath+"log.txt") #get mapped reads - os.system("samtools view -Sb -f 0x4 "+syspath+"map"+str(k)+".sam > "+syspath+"umapped"+str(k)+".bam 2>"+syspath+"log.txt") #get unmapped reads - os.system("samtools view -Sb -f 0x10 "+syspath+"map"+str(k)+".sam > "+syspath+"rmapped"+str(k)+".bam 2>"+syspath+"log.txt") #get reversed mapped reads - os.system("samtools merge -f "+syspath+"unmapped"+str(k)+".bam "+syspath+"umapped"+str(k)+".bam "+syspath+"rmapped"+str(k)+".bam") #get reversed mapped reads - os.system("samtools view -h -o "+syspath+"unmapped"+str(k)+".sam "+syspath+"unmapped"+str(k)+".bam") #get reversed mapped reads - if k>0: - os.system("samtools view -h -o "+syspath+"mapped"+str(k)+".sam "+syspath+"mapped"+str(k)+".bam") #get reversed mapped reads - os.system("cut -f 1 "+syspath+"unmapped"+str(k)+".sam > "+syspath+"unmapped"+str(k)+".txt") - os.system("cut -f 1 "+syspath+"mapped"+str(k)+".sam > "+syspath+"mapped"+str(k)+".txt") - os.system("python "+syspath+"remove_map.py "+syspath+"unmapped"+str(k)+".txt "+syspath+"mapped"+str(k)+".txt "+syspath+"runmapped"+str(k)+".txt") - os.system("rm "+syspath+"mapped"+str(k)+".sam") - os.system("rm "+syspath+"mapped"+str(k)+".txt") - os.system("rm "+syspath+"unmapped"+str(k)+".txt") - else: - os.system("cut -f 1 "+syspath+"unmapped"+str(k)+".sam > "+syspath+"runmapped"+str(k)+".txt") - - os.system("rm "+syspath+"unmapped"+str(k)+".bam") - os.system("rm "+syspath+"umapped"+str(k)+".bam") - os.system("rm "+syspath+"rmapped"+str(k)+".bam") - os.system("python "+syspath+"seq_track.py "+syspath+"runmapped"+str(k)+".txt "+syspath+"seq"+str(k)+".fa "+syspath+"unmap_seq"+str(k)+".fa "+tp) #get unmapped sequence - os.system("python "+syspath+"truncate.py "+syspath+"unmap_seq"+str(k)+".fa "+shift+" "+syspath+"seq"+str(k+1)+".fa "+length) #truncate unmapped sequence - os.system("rm "+syspath+"seq"+str(k)+".fa") #Remove sequences being mapped - os.system("rm "+syspath+"map"+str(k)+".sam") #Remove mapping file - os.system("rm "+syspath+"unmap_seq"+str(k)+".fa") #Remove unmapped sequnce - os.system("rm "+syspath+"runmapped"+str(k)+".txt") - os.system("rm "+syspath+"unmapped"+str(k)+".sam") - - os.system("wc -l "+syspath+"seq"+str(k+1)+".fa > "+syspath+"count"+str(k+1)+".txt") - c = read_sp_file(syspath+"count"+str(k+1)+".txt") - if c[0][0] == '0': #If no reads is in the sequence file, stop - os.system("rm "+syspath+"count"+str(k+1)+".txt") - os.system("rm "+syspath+"seq"+str(k+1)+".fa") - break - os.system("rm "+syspath+"count"+str(k+1)+".txt") - k = k+1 - -ss = "" -for i in range(0,k+1): - ss = ss+" "+syspath+"mapped"+str(i)+".bam" - - -os.system("samtools merge -f "+output_file+" "+ss) -#print("samtools merge mapped_all.bam"+ss) -os.system("rm "+syspath+"mapped*.bam") -os.system("rm "+syspath+"ref*") - -
--- a/Iterative_mapping/iterative_map.xml Mon Sep 15 14:52:20 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,80 +0,0 @@ -<tool id="iterative_map_pipeline" name="Iterative mapping" version="1.0"> - <description></description> - <command interpreter="python"> - #if $mapping_file.type == "user" - iterative_map.py $file_format.type $file_format.seq_file $reference_file $shift $length $mapping_file.type $output $mapping_file.param_v $mapping_file.param_five $mapping_file.param_three $mapping_file.param_k $mapping_file.param_a $mapping_file.param_m $mapping_file.param_best - #else - iterative_map.py $file_format.type $file_format.seq_file $reference_file $shift $length $mapping_file.type $output - #end if - </command> - <requirements> - <requirement type="package" version="1.61">biopython</requirement> - <requirement type="package" version="1.7">numpy</requirement> - <requirement type="package" version="0.1.18">samtools</requirement> - <requirement type="package" version="0.12.7">bowtie</requirement> - </requirements> - <inputs> - <conditional name="file_format"> - <param name="type" type="select" label="Format of the file of the reads (Default FASTQ)"> - <option value="fastq">FASTQ</option> - <option value="fasta">FASTA</option> - </param> - <when value="fastq"> - <param name="seq_file" type="data" format="fastq" label="Fastq file"/> - </when> - <when value="fasta"> - <param name="seq_file" type="data" format="fasta" label="Fasta file"/> - </when> - </conditional> - <param name="reference_file" type="data" format="fasta" label="Reference genome/transcriptome"/> - <param name="shift" type="integer" value="1" label="Number of nucleotide trimmed each round"/> - <param name="length" type="integer" value="21" label="Minimum requirement of read length for mapping"/> - <conditional name="mapping_file"> - <param name="type" type="select" label="Bowtie mapping flags (Default -v 0 -a --best --strata)"> - <option value="default">Default</option> - <option value="user">User specified</option> - </param> - <when value="default"/> - <when value="user"> - <param name="param_v" type="integer" value="0" label="Number of mismatches for SOAP-like alignment policy (-v)"/> - <param name="param_five" type="integer" value="0" label="Trim n bases from high-quality (left) end of each read before alignment (-5)"/> - <param name="param_three" type="integer" value="0" label="Trim n bases from high-quality (right) end of each read before alignment (-3)"/> - <param name="param_k" type="integer" value="1" label="Report up to n valid alignments per read (-k)"/> - <param name="param_a" type="boolean" checked="False" truevalue = "1" falsevalue = "0" label="Whether or not to report all valid alignments per read (-a)"/> - <param name="param_m" type="integer" value="-1" label="Suppress all alignments for a read if more than n reportable alignments exist (-m), -1 for unlimited"/> - <param name="param_best" type="boolean" checked="False" truevalue = "1" falsevalue = "0" label="Whether or not to make Bowtie guarantee that reported singleton alignments are 'best' in terms of stratum and in terms of the quality values at the mismatched positions (--best --strata)"/> - </when> - </conditional> - - </inputs> - <outputs> - <data name="output" type="data" format="bam"/> - </outputs> - - <help> - - -**TIPS**: - ------ - -**Input**: - -* 1. Sequence file type (FASTA/FASTQ) -* 2. Sequence file (fasta/fastq format) {Default: fastq file} -* 3. Reference file (e.g. cDNA library [fasta]) -* 4. “Shift” (The length of the sequence that will be trimmed at the 3’end of the reads before each round of mapping) -* 5. “Length” (The minimum length of the reads for mapping after trimming) -* [Optional] -* 1. Bowtie mapping flags (options) [Default: -v 0 -a --best --strata] (-v flag indicates the number of allowed mismatches. use -5/-3 flag to trim nucleotides from 5'/3' end of the reads) - ------ - -**Output**: - -A bam file with all of the reads that are mapped - - - - </help> -</tool>
--- a/Iterative_mapping/map_ex.py Mon Sep 15 14:52:20 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,31 +0,0 @@ -#!/usr/bin/env python -# -*- coding: utf-8 -*- - -import sys -from read_file import * -from Bio import SeqIO - -map_file = sys.argv[1] -result_file = sys.argv[2] - - -#reads = read_t_file(read_file); - -f = open(map_file); -h = file(result_file, 'w') - -for aline in f.readlines(): - tline = aline.strip(); - tl = tline.split('\t'); - if len(tl)>4: - if int(tl[1].strip())== 0: - h.write(tline) - h.write('\n') - - -f.close(); -h.close() - - - -
--- a/Iterative_mapping/read_file.py Mon Sep 15 14:52:20 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,21 +0,0 @@ -#!/usr/bin/env python -# -*- coding: utf-8 -*- - -import sys - - - -def read_t_file(in_file): - f = open(in_file); - result = []; - for aline in f.readlines(): - temp = []; - tline = aline.strip(); - tl = tline.split('\t'); - for i in range(0, len(tl)): - temp.append(tl[i].strip()); - result.append(temp); - f.close(); - return result; - -
--- a/Iterative_mapping/read_s_file.py Mon Sep 15 14:52:20 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,22 +0,0 @@ -#!/usr/bin/env python -# -*- coding: utf-8 -*- - -import sys - - - -def read_sp_file(in_file): - f = open(in_file); - result = []; - for aline in f.readlines(): - temp = []; - tline = aline.strip(); - tl = tline.split(' '); - for i in range(0, len(tl)): - if len(tl[i].strip())>0: - temp.append(tl[i].strip()); - result.append(temp); - f.close(); - return result; - -
--- a/Iterative_mapping/remove_map.py Mon Sep 15 14:52:20 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,29 +0,0 @@ -#!/usr/bin/env python -# -*- coding: utf-8 -*- - -import sys -from read_file import * - - -unmap_file = sys.argv[1] -map_file = sys.argv[2] -result_file = sys.argv[3] - - -unmap = read_t_file(unmap_file) -mapped = read_t_file(map_file) -h = file(result_file, 'w') - -maps = set() -for i in range(len(mapped)): - maps.add(mapped[i][0]) - - -for i in range(len(unmap)): - name = unmap[i][0] - if name not in maps: - h.write(name) - h.write('\n') - - -h.close()
--- a/Iterative_mapping/seq_track.py Mon Sep 15 14:52:20 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,38 +0,0 @@ -#!/usr/bin/env python -# -*- coding: utf-8 -*- - -import sys -from read_file import * -from Bio import SeqIO - -unmap_file = sys.argv[1] -reads_file = sys.argv[2] -result_file = sys.argv[3] -tp = sys.argv[4] - - -unmap = read_t_file(unmap_file); - -h = file(result_file, 'w') - -reads = SeqIO.parse(reads_file,tp) -um = set() -for i in range(0, len(unmap)): - id_r = unmap[i][0] - um.add(id_r) - -for read in reads: - if read.id in um: - h.write('>') - h.write(read.id) - h.write('\n') - h.write(read.seq.tostring()) - h.write('\n') - - - -h.close() - - - -
--- a/Iterative_mapping/tool_dependencies.xml Mon Sep 15 14:52:20 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,15 +0,0 @@ -<?xml version="1.0"?> -<tool_dependency> - <package name="biopython" version="1.61"> - <repository changeset_revision="ae9dda584395" name="package_biopython_1_61" owner="biopython" prior_installation_required="True" toolshed="http://toolshed.g2.bx.psu.edu" /> - </package> - <package name="numpy" version="1.7"> - <repository changeset_revision="ef12a3a11d5b" name="package_numpy_1_7" owner="iuc" prior_installation_required="False" toolshed="http://toolshed.g2.bx.psu.edu" /> - </package> - <package name="samtools" version="0.1.18"> - <repository changeset_revision="171cd8bc208d" name="package_samtools_0_1_18" owner="devteam" prior_installation_required="False" toolshed="http://toolshed.g2.bx.psu.edu" /> - </package> - <package name="bowtie" version="0.12.7"> - <repository changeset_revision="9f9f38617a98" name="package_bowtie_0_12_7" owner="devteam" prior_installation_required="False" toolshed="http://toolshed.g2.bx.psu.edu" /> - </package> -</tool_dependency>
--- a/Iterative_mapping/truncate.py Mon Sep 15 14:52:20 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,32 +0,0 @@ -#!/usr/bin/env python -# -*- coding: utf-8 -*- - -import sys -from Bio import SeqIO - -fasta_file = sys.argv[1] -shift_in = sys.argv[2] -result_file = sys.argv[3] -length = sys.argv[4] - -shift = int(shift_in) - -fasta_sequences = SeqIO.parse(open(fasta_file),'fasta'); -h = file(result_file,'w') -for seq in fasta_sequences: - nuc = seq.id; - sequence = seq.seq.tostring(); - if (len(sequence)-shift)>=int(length): - h.write('>'+nuc) - h.write('\n') - h.write(sequence[0:(len(sequence)-shift)]) - h.write('\n') - - - - -h.close() - - - -
--- a/Iterative_mapping/unmap.py Mon Sep 15 14:52:20 2014 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,31 +0,0 @@ -#!/usr/bin/env python -# -*- coding: utf-8 -*- - -import sys -from read_file import * -from Bio import SeqIO - -map_file = sys.argv[1] -result_file = sys.argv[2] - - -#reads = read_t_file(read_file); - -f = open(map_file); -h = file(result_file, 'w') - -for aline in f.readlines(): - tline = aline.strip(); - tl = tline.split('\t'); - if len(tl)>4: - if int(tl[1].strip()) != 0: - h.write(tl[0].strip()); - h.write('\n'); - - -f.close(); -h.close() - - - -
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/predict/parse_dis_pac.py Mon Sep 15 14:52:43 2014 -0400 @@ -0,0 +1,43 @@ +#parse reactivity file into a dictionary + +import sys + +def parse_dist(in_file): + result = [] + distribution = {} + name = [] + f = open(in_file) + for aline in f.readlines(): + line = aline.strip() + dis = line.strip() + dist = dis.split('\t') #split the line and the reactivites or reads are in a list + if len(dist) > 0: + if len(dist) == 1: + if dist[0].strip().find('coverage')==-1: + name.append(line) #add the name in the name list + flag = 1 + t_name = line + else: + distri = [] + for i in range(0, len(dist)): + distri.append(dist[i].strip()) + distribution[t_name] = distri #add the list of reactivities into a dictionary + result.append(name) + result.append(distribution) #Output the dictionary + f.close() + return result + + + + + + + + + + + + + + +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/predict/predict_RNAs.py Mon Sep 15 14:52:43 2014 -0400 @@ -0,0 +1,93 @@ +#RNA structure prediction & Output and illustrate reactivities + +import sys +from parse_dis_pac import * +from read_file import * +from Bio import SeqIO +import os +from rtts_plot import * + + +id_file = sys.argv[1] +seq_file = sys.argv[2] +output_file = sys.argv[4] + + +flag = 0 +if sys.argv[3]!='None': #input reactivity file if provided + react_file = sys.argv[3] + react = parse_dist(react_file) + react = react[1] + flag = 1 + +ospath = os.path.realpath(sys.argv[0]) +ost = ospath.split('/') +syspath = "" +for i in range(len(ost)-1): + syspath = syspath+ost[i].strip() + syspath = syspath+'/' + +ids = read_t_file(id_file) +sequences = SeqIO.parse(seq_file, 'fasta') + + +seqs = {} +for seq in sequences: + seqs[seq.id] = seq.seq.tostring() + +if len(ids)>10: #setup a limit of the number of sequence to be predicted + print("Number of sequences exceeds limitation!") + sys.exit(0) + + +#predict RNA structures +os.system("mkdir "+syspath+"output_f") +for i in range(len(ids)): + id_s = ids[i][0] + print(id_s) + #Put RNA sequence and reactivities into files + if id_s in seqs: + f = file(syspath+"temp.txt", 'w') + f.write('>'+id_s) + f.write('\n') + f.write(seqs[id_s]) + f.close() + if flag == 0: + os.system("Fold "+syspath+"temp.txt"+" "+syspath+"output_f/"+id_s+".ct") + if flag == 1: + if id_s in react: + f = file(syspath+"constraint.txt",'w') + make_plot(react[id_s],id_s,(syspath+"output_f/")) #make a plot of the distribution of the reactivites of the input RNA + #h = file(syspath+"output_f/transcript_reactivities.txt", 'w') + #h.write(id_s) + #h.write('\n') + for j in range(0, (len(react[id_s]))): + if react[id_s][j]!='NA': + f.write(str(j+1)) + f.write('\t') + f.write(str(react[id_s][j])) + f.write('\n') + #h.write(str(react[id_s][j])) #Output the reactivities + #h.write('\t') + f.close() + #h.write('\n') + #h.write('\n') + os.system("Fold "+syspath+"temp.txt"+" -sh"+" "+syspath+"constraint.txt"+" "+syspath+"output_f/"+id_s+".ct") + else: + print(id_s+" not in the data of react!") + os.system("draw "+syspath+"output_f/"+id_s+".ct "+syspath+"output_f/"+id_s+".ps") + else: + print(id_s+" not in the data of sequences!") + +#Remove the unnecessary files +os.system("tar -zcvPf "+output_file+" "+syspath+"output_f/"+"*.*") +os.system("rm -f "+syspath+"temp.txt") +os.system("rm -r "+syspath+"output_f") +if flag == 1: + os.system("rm -f "+syspath+"constraint.txt") + # h.close() + + + + +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/predict/predict_RNAs.xml Mon Sep 15 14:52:43 2014 -0400 @@ -0,0 +1,63 @@ +<tool id="predict_pipeline" name="RNA structure prediction" version="1.0"> + <description></description> + <command interpreter="python">predict_RNAs.py $rna_list $reference_file $reactivity_file $output </command> + <requirements> + <requirement type="package" version="1.61">biopython</requirement> + <requirement type="package" version="1.7">numpy</requirement> + </requirements> + <inputs> + <param name="rna_list" type="data" format="txt" label="List of RNA ids to predict"/> + <param name="reference_file" type="data" format="fasta" label="Reference genome/transcriptome"/> + <param name="reactivity_file" type="data" optional = "true" label="Reactivity"/> +<!--- + <conditional name="reactivity"> + <param name="choose" type="select" label="Reactivity files exists?"> + <option value="N">No</option> + <option value="Y">Yes</option> + </param> + <when value="Y"> + <param name="reactivity_file" type="data" optional = "true" label="Reactivity"/> + </when> + <when value="N"/> + </conditional> + +--> + </inputs> + <outputs> + <data name="output" format=".tgz"/> + </outputs> + + <help> + + +**TIPS**: + +----- + +**Input**: + +* 1. A file with transcript Ids (Max num. 20), (each ID one line) +* 2. Reference file (fasta) used to map the reads +* [Optional]: +* 1. A reactivity file with structural reactivity for each nucleotide on the sequence provided + +----- + +**Output**: + +* 1. .ct files with predicted RNA structures [transciptID.ct] +* 2. .ps files which depict the predicted RNA structures [[transciptID.ps] +* [Optional] +* 3. .png files that shows the distribution of the reactivity of each nucleotide on the transcripts of interest. [transciptID.png] +* 4. A .txt file that includes the reactivities of all the nucleotides on the transcripts of interest. [transciptID.txt] + +----- + +**Attention** + +Make sure any of the transcript Ids does not contain "|" or space! + + + + </help> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/predict/rRNA.txt Mon Sep 15 14:52:43 2014 -0400 @@ -0,0 +1,8 @@ +>25s rRNA 3375nts +GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGTAGTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCGTCGTGCCCGGACCCTGTCGCACCACGAGGCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGATGCGTCCTGGTCGGATGCGGAACGGAGCAATCCGGTCCGCCGATCGATTCGGGGCGTGGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGGGCTTTTGATACGCTTGTGGAGACGTCGCTGCCGTGATCGTGGTCTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGGCGTCGGCCTGTGGGCTCCCCATTCGACCCGTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCTCGCGGGTGCACCGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGGACGCGAGTTCTATCGGGTAAAGCCAATGATTAGAGGCATTGGGGGCGCAACGCCTCGACCTATTCTCAAACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTTAAGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGGCGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGAACTTCGAAAGGGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTTGACGGCAACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTCGCGTGGTGTCCGGTGCGCCCCCGGGCGCCCTTGAAAATCCGGAGGACCGAGTGCCGCTCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCTCGGGGGTCCCAGTTCCGAACCCGTCGGCTGTCAGCGGACTGCTCGAGCTGCTTCCGCGGCGAGAGCGGGTCGCCGGCTGCCGGCCGGGGGACGACTGGGAACGGCTCTCTCGGGAGCTTTCCCCGGGCGTCGAACAGTCAGCTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTAACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTGTCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGAGAGGTGTAGGATAAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTTTTAACGTTATTTTACTTACTCCGTGAATCGGAGGCCGGGGTACAACCCCTGTTTTTGGTCCCAAGGCTCGCTTCGGCGGGTCGATCCGGGCGGAGGACATTGTCAGGTGGGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGAGAACAGAAATCTCGTGTGGAACAAAAGGGTAAAAGCTCGTTTGATTCTGATTTTCAGTACGAATACGAACCGTGAAAGCGTGGCCTATCGATCCTTTAGACTTCGGAATTTGAAGCTAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGTGTTGGATTGTTCACCCACCAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGATTATGACTGAACGCCTCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGCGCCCGCCGCCCGATTGCCGACCCTCAGTAGGAGCTTAGGCTCCAAAGGCACGTGTCGTTGGCTAAGTCCGTTCGGCGGAACGGTCGTTCGGACCGCCTTGAATTATAATTACCACCGAGCGGCGGGTAGAATCCTTTGCAGACGACTTAAATACGCGACGGGGTATTGTAAGTGGCAGAGTGGCCTTGCTGCCACGATCCACTGAGATTCAGCCCTTTGTCGCTAAGATTCGA +>gi|20197903:2706-4513 Arabidopsis thaliana chromosome 2 BAC F23H14 genomic sequence, complete sequence +TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTGTTTGATGGTAACTACTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTATGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTCTGCCCGTTGCTCTGATGATTCATGATAACTCGACGGATCGCATGGCCTCTGTGCTGGCGACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTGGTAACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACTGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAATGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCACGGCCGGCTTCTTAGAGGGACTATGGCCGTTTAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTCACACCTTGGCCGACAGGCCCGGGTAATCTTTGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGATCCGGTGAAGTGTTCGGATCGCGGCGACGTGGGTGGTTCGCCGCCCGCGACGTCGCGAGAAGTCCACTAAACCTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTG +>Arabidopsis thaliana 1 +GGATGCGATCATACCAGCACTAATGCACCGGATCCCATCAGAACTCCGCAGTTAAGCGTGCTTGGGCGAGAGTAGTACTAGGATGGGTGACCTCCTGGGAAGTCCTCGTGTTGCATCCCTC +>gi|186498419|ref|NR_022453.1| Arabidopsis thaliana (AT2G01020) rRNA +AAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCCAAGCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACAA \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/predict/rRNA_react.txt Mon Sep 15 14:52:43 2014 -0400 @@ -0,0 +1,8 @@ +gi|186498419|ref|NR_022453.1| +0.0349942738544 0.219596062195 0.0958706408233 0.194395004716 0.165304592733 NA 0.649363521545 0.004703371147 NA 0.0 NA 0.0 NA NA 0.226052814765 0.145104959196 0.235114323931 0.590973505942 NA NA 0.671823965086 NA 0.600180467352 NA 0.0 NA 0.67204697444 NA NA 0.0 NA 0.0 NA 0.0 NA 0.0 0.0905633447413 NA 0.0844580185128 NA 0.0 NA NA 0.387056722073 0.063190678881 NA 0.302648296659 0.123623615737 0.0 NA NA 0.288529445998 NA 0.0 NA 0.272682267617 1.18142319227 0.222698183429 NA NA 0.0 NA 0.308507027145 NA 0.0 0.0 NA NA NA NA NA NA NA NA 1.09905990052 0.331081493258 NA NA NA 0.0 0.316741455 NA 0.152869347497 0.358830282006 NA 0.172524825658 0.0232297542077 0.397612802938 NA NA NA 0.591410529537 0.531312624449 0.0105725487651 0.0 0.544371401206 NA 0.764412925075 NA 1.31480559071 NA NA 0.338476420086 NA NA NA NA 0.315926121887 0.0 0.0 NA 0.0 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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/predict/read_file.py Mon Sep 15 14:52:43 2014 -0400 @@ -0,0 +1,21 @@ +#!/usr/bin/env python +# -*- coding: utf-8 -*- + +import sys + + + +def read_t_file(in_file): + f = open(in_file); + result = []; + for aline in f.readlines(): + temp = []; + tline = aline.strip(); + tl = tline.split('\t'); + for i in range(0, len(tl)): + temp.append(tl[i].strip()); + result.append(temp); + f.close(); + return result; + +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/predict/rtts_plot.py Mon Sep 15 14:52:43 2014 -0400 @@ -0,0 +1,56 @@ +#!/usr/bin/env python +#Make a plot of reactivity distribution + +import sys +import numpy as np +import matplotlib +from pylab import * +import math + +#Convert the reactivities (Make NA to 0) +def convert_react(a): + r = [] + for i in range(len(a)): + if a[i]!='NA': + r.append(float(a[i])) + else: + r.append(float(0)) + return r + + +#Make a plot of the distribution +def make_plot(ar,id_s,path): + N = len(ar) + a = convert_react(ar) + w = 1 + ind = np.arange(N) + + fig = figure() + fig, ax = subplots() + ax.bar(ind+w, a, width = w, color = 'r',edgecolor = 'r') + ax.set_ylabel('DMS Reactivity') + ax.set_xlabel('Nucleotide Index') + + + mag = int(math.log(N,10))-1 + tail = 10**mag + + intervel = int(math.ceil(float(N)/tail)/5) + print(N) + print(intervel) + tl = [] + k = 0 + ax.set_xticks(np.arange(0,N,intervel*tail)) + print(np.arange(0,N,intervel*tail)) + ax.set_xticklabels(np.arange(0,N,intervel*tail)) + + ax.set_title(id_s+" reactivity distribution") + savefig(path+id_s+'.tif') + + + + + + + +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/predict/tool_dependencies.xml Mon Sep 15 14:52:43 2014 -0400 @@ -0,0 +1,9 @@ +<?xml version="1.0"?> +<tool_dependency> + <package name="biopython" version="1.61"> + <repository changeset_revision="ae9dda584395" name="package_biopython_1_61" owner="biopython" prior_installation_required="True" toolshed="http://toolshed.g2.bx.psu.edu" /> + </package> + <package name="numpy" version="1.7"> + <repository changeset_revision="ef12a3a11d5b" name="package_numpy_1_7" owner="iuc" prior_installation_required="False" toolshed="http://toolshed.g2.bx.psu.edu" /> + </package> +</tool_dependency>