diff omero_roi_upload.py @ 0:9051b91545f6 draft

planemo upload for repository https://github.com/Helmholtz-UFZ/galaxy-tools/tree/main/tools/omero commit 5b1b30d409355cee98485815c1dd4ac48649bcc1

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/omero_roi_upload.py	Thu Sep 05 11:55:44 2024 +0000
@@ -0,0 +1,165 @@
+import argparse
+import re
+
+import pandas as pd
+from ezomero import connect, post_roi
+from ezomero.rois import Ellipse, Label, Line, Point, Polygon, Polyline, Rectangle
+
+
+def parse_color(color_str):
+    if not color_str:
+        return None
+    return tuple(map(int, re.findall(r'\d+', color_str)))
+
+
+def parse_points(points_str):
+    if not points_str:
+        return None
+    # Remove leading and trailing brackets and split into individual points
+    points_str = points_str.strip("[]")
+    points = points_str.split("),(")
+    points = [point.strip("()") for point in points]  # Remove any remaining parentheses
+    return [tuple(map(float, point.split(','))) for point in points]
+
+
+def create_shape(row):
+    shape_type = row['shape']
+    shape = None
+
+    if shape_type == 'Ellipse':
+        shape = Ellipse(
+            x=row['x'],
+            y=row['y'],
+            x_rad=row['x_rad'],
+            y_rad=row['y_rad'],
+            z=row.get('z'),
+            c=row.get('c'),
+            t=row.get('t'),
+            fill_color=parse_color(row.get('fill_color')),
+            stroke_color=parse_color(row.get('stroke_color')),
+            stroke_width=row.get('stroke_width')
+        )
+    elif shape_type == 'Label':
+        shape = Label(
+            x=row['x'],
+            y=row['y'],
+            label=row['label'],
+            fontSize=row['fontSize'],
+            z=row.get('z'),
+            c=row.get('c'),
+            t=row.get('t'),
+            fill_color=parse_color(row.get('fill_color')),
+            stroke_color=parse_color(row.get('stroke_color')),
+            stroke_width=row.get('stroke_width')
+        )
+    elif shape_type == 'Line':
+        shape = Line(
+            x1=row['x1'],
+            y1=row['y1'],
+            x2=row['x2'],
+            y2=row['y2'],
+            markerStart=row.get('markerStart', None),
+            markerEnd=row.get('markerEnd', None),
+            label=row.get('label', None),
+            z=row.get('z'),
+            c=row.get('c'),
+            t=row.get('t'),
+            fill_color=parse_color(row.get('fill_color')),
+            stroke_color=parse_color(row.get('stroke_color')),
+            stroke_width=row.get('stroke_width')
+        )
+    elif shape_type == 'Point':
+        shape = Point(
+            x=row['x'],
+            y=row['y'],
+            z=row.get('z'),
+            c=row.get('c'),
+            t=row.get('t'),
+            fill_color=parse_color(row.get('fill_color')),
+            stroke_color=parse_color(row.get('stroke_color')),
+            stroke_width=row.get('stroke_width')
+        )
+    elif shape_type == 'Polygon':
+        shape = Polygon(
+            points=parse_points(row['points']),
+            z=row.get('z'),
+            c=row.get('c'),
+            t=row.get('t'),
+            fill_color=parse_color(row.get('fill_color')),
+            stroke_color=parse_color(row.get('stroke_color')),
+            stroke_width=row.get('stroke_width')
+        )
+    elif shape_type == 'Polyline':
+        shape = Polyline(
+            points=parse_points(row['points']),
+            z=row.get('z'),
+            c=row.get('c'),
+            t=row.get('t'),
+            fill_color=parse_color(row.get('fill_color')),
+            stroke_color=parse_color(row.get('stroke_color')),
+            stroke_width=row.get('stroke_width')
+        )
+    elif shape_type == 'Rectangle':
+        shape = Rectangle(
+            x=row['x'],
+            y=row['y'],
+            width=row['width'],
+            height=row['height'],
+            z=row.get('z'),
+            c=row.get('c'),
+            t=row.get('t'),
+            fill_color=parse_color(row.get('fill_color')),
+            stroke_color=parse_color(row.get('stroke_color')),
+            stroke_width=row.get('stroke_width')
+        )
+    return shape
+
+
+def main(input_file, conn, image_id, log_file):
+    # Open log file
+    with open(log_file, 'w') as log:
+        df = pd.read_csv(input_file, sep='\t')
+        for index, row in df.iterrows():
+            msg = f"Processing row {index + 1}/{len(df)}: {row.to_dict()}"
+            print(msg)
+            log.write(msg + "\n")
+            shape = create_shape(row)
+            if shape:
+                roi_name = row['roi_name'] if 'roi_name' in row else None
+                roi_description = row['roi_description'] if 'roi_description' in row else None
+                roi_id = post_roi(conn, image_id, [shape], name=roi_name, description=roi_description)
+                msg = f"ROI ID: {roi_id} for row {index + 1}"
+                print(msg)
+                log.write(msg + "\n")
+            else:
+                msg = f"Skipping row {index + 1}: Unable to create shape"
+                print(msg)
+                log.write(msg + "\n")
+
+
+if __name__ == "__main__":
+    parser = argparse.ArgumentParser(
+        description="Create shapes from a tabular file and optionally post them as an ROI to OMERO.")
+    parser.add_argument("--input_file", help="Path to the input tabular file.")
+    parser.add_argument("--image_id", type=int, required=True, help="ID of the image to which the ROI will be linked")
+    parser.add_argument("--host", type=str, required=True, help="OMERO server host")
+    parser.add_argument("--user", type=str, required=True, help="OMERO username")
+    parser.add_argument("--psw", type=str, required=True, help="OMERO password")
+    parser.add_argument("--port", type=int, default=4064, help="OMERO server port")
+    parser.add_argument("--log_file", type=str, default="process.txt", help="Log file path")
+
+    args = parser.parse_args()
+
+    conn = connect(
+        host=args.host,
+        user=args.user,
+        password=args.psw,
+        port=args.port,
+        group="",
+        secure=True
+    )
+
+    try:
+        main(args.input_file, conn, args.image_id, args.log_file)
+    finally:
+        conn.close()