# HG changeset patch # User ulfschaefer # Date 1450700568 18000 # Node ID 7ec666e2f315d3b69d960931ab321666f31945cd # Parent 59eca98715b4418a49f940a1149c1124769a3442 Deleted selected files diff -r 59eca98715b4 -r 7ec666e2f315 phe_samtools_mpileup.sh --- a/phe_samtools_mpileup.sh Mon Dec 21 07:22:34 2015 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,22 +0,0 @@ -#!/bin/bash - -echo $@ - -SAM=$1 -shift -REF=$1 -shift -OUTVCF=$1 - -TMPBAM=$$.tmpbam -TMPBAM2=$$.tmpbam2 - -samtools view -bS $SAM > $TMPBAM -samtools sort $TMPBAM $TMPBAM2 - -CMD="samtools mpileup -t DP,DV,DP4,DPR,SP -Auf $REF $TMPBAM2.bam | bcftools call -m -f GQ > $OUTVCF" - -echo "CMD: "$CMD -eval $CMD -rm $TMPBAM -rm $TMPBAM2.bam diff -r 59eca98715b4 -r 7ec666e2f315 phe_samtools_mpileup.xml --- a/phe_samtools_mpileup.xml Mon Dec 21 07:22:34 2015 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,32 +0,0 @@ - - PHE SNP and indel caller - - samtools - - - phe_samtools_mpileup.sh $input_sam $ref_file $outvcf - - - - - - - - - - - - - - - - - -**Citation** - -For the underlying tool, please cite `Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. <http://www.ncbi.nlm.nih.gov/pubmed/19505943>`_ - -If you use this tool in Galaxy, please cite Blankenberg D, et al. *In preparation.* - - -