Mercurial > repos > ulfschaefer > vcfs2fasta
diff vcfs2fasta.xml @ 22:96f393ad7fc6 draft default tip
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author | ulfschaefer |
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date | Wed, 23 Dec 2015 04:50:58 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/vcfs2fasta.xml Wed Dec 23 04:50:58 2015 -0500 @@ -0,0 +1,123 @@ +<tool id="vcfs2fasta" name="VCFs to fasta" version="1.0"> + <description>Takes a set of VCF files and outputs a multi fasta file with only the variant positions.</description> + <requirements> + <requirement type="package" version="2.7.10">python</requirement> + <requirement type="package" version="0.6.8dev">pyvcf</requirement> + <requirement type="package" version="3.11">pyyaml</requirement> + <requirement type="package" version="2.0.2">bintrees</requirement> + <requirement type="package" version="1.66">biopython</requirement> + <requirement type="package" version="1.4">matplotlib</requirement> + </requirements> + <stdio> + <!-- Assume anything other than zero is an error --> + <exit_code range="1:" /> + <exit_code range=":-1" /> + </stdio> + <command interpreter="bash"> + vcfs2fasta.sh + $output + #if str($mix_cond.mix) == "yes": + $mix_cond.mix_value + #else + NOTTHERE + #end if + #if str($cols_cond.cols) == "yes": + $cols_cond.column_ns + #else + NOTTHERE + #end if + #if str($sample_cond.sample) == "yes": + $sample_cond.sample_ns + #else + NOTTHERE + #end if + #if str($reference_cond.reference) == "yes": + $reference_cond.ref_fa + #else + NOTTHERE + #end if + #if str($include_cond.include) == "yes": + $include_cond.in_bed + #else + NOTTHERE + #end if + #if str($exclude_cond.exclude) == "yes": + $exclude_cond.ex_bed + #else + NOTTHERE + #end if + #for $i, $input_vcf in enumerate( $input_vcfs ): + "${input_vcf}" + #end for + </command> + + <inputs> + <param name="input_vcfs" type="data" multiple="true" format="vcf" label="Input VCF file(s)" /> + <conditional name="mix_cond"> + <param name="mix" type="select" label="With Mixtures" help="Specify this option with a threshold to output mixtures above this threshold."> + <option value="yes">Specify</option> + <option value="no" selected="true">Do not specify</option> + </param> + <when value="yes"> + <param name="mix_value" type="float" value="0.5" label="Mixture value" /> + </when> + </conditional> + <conditional name="cols_cond"> + <param name="cols" type="select" label="Column Ns" help="Keeps columns with fraction of Ns above specified threshold."> + <option value="yes">Specify</option> + <option value="no" selected="true">Do not specify</option> + </param> + <when value="yes"> + <param name="column_ns" type="float" value="0.5" label="Column Ns value" /> + </when> + </conditional> + <conditional name="sample_cond"> + <param name="sample" type="select" label="Sample Ns" help="Keeps samples with fraction of Ns above specified threshold."> + <option value="yes">Specify</option> + <option value="no" selected="true">Do not specify</option> + </param> + <when value="yes"> + <param name="sample_ns" type="float" value="0.5" label="Sample Ns value" /> + </when> + </conditional> + <conditional name="reference_cond"> + <param name="reference" type="select" label="Reference genome file" help="If path to reference specified, then whole genome will be outputted."> + <option value="yes">Specify</option> + <option value="no" selected="true">Do not specify</option> + </param> + <when value="yes"> + <param name="ref_fa" type="data" format="fasta" label="Reference fasta file" help="Fasta format"/> + </when> + </conditional> + <conditional name="include_cond"> + <param name="include" type="select" label="Include region" help="Specify regions to include in a bed file."> + <option value="yes">Specify</option> + <option value="no" selected="true">Do not specify</option> + </param> + <when value="yes"> + <param name="in_bed" type="data" format="bed" label="Include regions bed file" help="bed format"/> + </when> + </conditional> + <conditional name="exclude_cond"> + <param name="exclude" type="select" label="Exclude region" help="Specify regions to exclude in a bed file."> + <option value="yes">Specify</option> + <option value="no" selected="true">Do not specify</option> + </param> + <when value="yes"> + <param name="ex_bed" type="data" format="bed" label="Exclude regions bed file" help="bed format"/> + </when> + </conditional> + </inputs> + + <outputs> + <data format="fasta" name="output" label="${tool.name} on ${on_string}: FASTA file" /> + </outputs> + <test> + <param name="input_vcfs" value="1_short.vcf" ftype="vcf" /> + <param name="input_vcfs" value="2_short.vcf" ftype="vcf" /> + <output name="output" file="testresult.fa" ftype="fasta" /> + </test> + <help> + + </help> +</tool>