Mercurial > repos > urgi-team > teiso
diff TEisotools-1.1.a/TEiso/Bowtie_build.py @ 16:836ce3d9d47a draft default tip
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author | urgi-team |
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date | Thu, 21 Jul 2016 07:42:47 -0400 |
parents | 255c852351c5 |
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--- a/TEisotools-1.1.a/TEiso/Bowtie_build.py Thu Jul 21 07:36:44 2016 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,123 +0,0 @@ -#!/usr/bin/env python - -# Copyright INRA (Institut National de la Recherche Agronomique) -# http://www.inra.fr -# http://urgi.versailles.inra.fr -# -# This software is governed by the CeCILL license under French law and -# abiding by the rules of distribution of free software. You can use, -# modify and/ or redistribute the software under the terms of the CeCILL -# license as circulated by CEA, CNRS and INRIA at the following URL -# "http://www.cecill.info". -# -# As a counterpart to the access to the source code and rights to copy, -# modify and redistribute granted by the license, users are provided only -# with a limited warranty and the software's author, the holder of the -# economic rights, and the successive licensors have only limited -# liability. -# -# In this respect, the user's attention is drawn to the risks associated -# with loading, using, modifying and/or developing or reproducing the -# software by the user in light of its specific status of free software, -# that may mean that it is complicated to manipulate, and that also -# therefore means that it is reserved for developers and experienced -# professionals having in-depth computer knowledge. Users are therefore -# encouraged to load and test the software's suitability as regards their -# requirements in conditions enabling the security of their systems and/or -# data to be ensured and, more generally, to use and operate it in the -# same conditions as regards security. -# -# The fact that you are presently reading this means that you have had -# knowledge of the CeCILL license and that you accept its terms. -import os -from commons.core.checker.CheckerUtils import CheckerUtils -from commons.core.utils.RepetOptionParser import RepetOptionParser -from commons.core.utils.FileUtils import FileUtils -import subprocess - -class Bowtie_build(object): - - def __init__(self, input_reference = "", output_prefix= "", workingDir = "", verbosity = 3): - - self._inputRef = input_reference - self._outPrefix = output_prefix - self._outputDir = workingDir - self._verbosity = verbosity - - def setAttributesFromCmdLine(self): - description = "Create index for refrence file with bowtie-build.\n" - usage = "Bowtie_build -i <reference_in> -o <ebwt_outfile_base>\n" - print usage - parser = RepetOptionParser(description = description, usage = usage) - parser.add_option( '-i', '--input_reference', dest='input_reference', help='comma-separated list of files with ref sequences' ) - parser.add_option( '-p', '--output_prefix', dest='output_prefix', help=' write Ebwt data to files with this dir/basename' ) - parser.add_option( '-o', '--outputDir', dest='outputDir', help='result directory name', default = "") - options, args = parser.parse_args() - self.setAttributesFromOptions(options) - - def setAttributesFromOptions(self, options): - self._inputRef = options.input_reference - self._outPrefix = options.output_prefix - self._outputDir = options.outputDir - - def checkExecutables(self): - if not CheckerUtils.isExecutableInUserPath("bowtie2-build"): - raise Exception("ERROR: bowtie2-build must be in your path") - - def checkOptions(self): - if self._inputRef != "": - if not FileUtils.isRessourceExists(self._inputRef): - raise Exception("ERROR: reference file %s does not exist!" % self._inputRef) - else: - raise Exception("ERROR: No specified -i option!") - - if self._outPrefix == "": - raise Exception("ERROR: No specified -o option!") - - - def getBowtie2buildCmd(self, inputRef, outPrefix ): - cmd = 'bowtie2-build %s %s' % (inputRef, self._outPrefix ) - #print cmd - return cmd - - def run(self): - - self.checkExecutables() - self.checkOptions() - try: - workingDir = self._outputDir - if os.path.exists(workingDir): - raise Exception("ERROR: %s already exists." % workingDir) - os.mkdir(workingDir) - os.chdir(workingDir) - os.symlink("%s" % self._inputRef,"%s.fa" % self._outPrefix) - cmd_bowtie = self.getBowtie2buildCmd(self._inputRef, self._outPrefix) - ## hide output of subprocess: stdout = index_dir_stderr - fstdout = open( "bowtie2_build.log" , 'w' ) - process = subprocess.Popen(cmd_bowtie, shell = True, stdout = fstdout, stderr=subprocess.STDOUT) - returncode = process.wait() - fstdout.close() - # get stderr, allowing for case where it's very large - fstdout = open("bowtie2_build.log", 'rb' ) - stderr = '' - buffsize = 1048576 - try: - while True: - stderr += fstdout.read( buffsize ) - if not stderr or len( stderr ) % buffsize != 0: - break - except OverflowError: - pass - fstdout.close() - if returncode != 0: - raise Exception, stderr - except Exception: - raise Exception("ERROR in %s " % cmd_bowtie) - - -if __name__ == "__main__": - print "heloo" - iLaunch = Bowtie_build() - iLaunch.setAttributesFromCmdLine() - iLaunch.run() -