view TEisotools-1.0/TEiso/ClosestToStartSite.py @ 10:b6db3eaf35a5 draft

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#!/usr/bin/env python

# Copyright INRA (Institut National de la Recherche Agronomique)
# http://www.inra.fr
# http://urgi.versailles.inra.fr
#
# This software is governed by the CeCILL license under French law and
# abiding by the rules of distribution of free software.  You can  use,
# modify and/ or redistribute the software under the terms of the CeCILL
# license as circulated by CEA, CNRS and INRIA at the following URL
# "http://www.cecill.info".
#
# As a counterpart to the access to the source code and  rights to copy,
# modify and redistribute granted by the license, users are provided only
# with a limited warranty  and the software's author,  the holder of the
# economic rights,  and the successive licensors  have only  limited
# liability.
#
# In this respect, the user's attention is drawn to the risks associated
# with loading,  using,  modifying and/or developing or reproducing the
# software by the user in light of its specific status of free software,
# that may mean  that it is complicated to manipulate,  and  that  also
# therefore means  that it is reserved for developers  and  experienced
# professionals having in-depth computer knowledge. Users are therefore
# encouraged to load and test the software's suitability as regards their
# requirements in conditions enabling the security of their systems and/or
# data to be ensured and,  more generally, to use and operate it in the
# same conditions as regards security.
#
# The fact that you are presently reading this means that you have had
# knowledge of the CeCILL license and that you accept its terms.

from commons.core.LoggerFactory             import LoggerFactory
from commons.core.utils.RepetOptionParser   import RepetOptionParser
from commons.core.checker.RepetException    import RepetException
from commons.core.utils.FileUtils           import FileUtils
import re,os
LOG_NAME = "TEiso"

class ClosestToStartSite(object):
    
    def __init__(self, inputFile = "", cuffcom_tmap =   "", outputFile = "", verbosity = 3):
        self._inputFile = inputFile
        self._cuffcom_tmap = cuffcom_tmap
        self._outputFile = outputFile
        self._verbosity = verbosity
        self._log = LoggerFactory.createLogger("%s.%s" % (LOG_NAME, self.__class__.__name__), self._verbosity)
            
    def setAttributesFromCmdLine(self):
        self._toolVersion = "1.0"
        description = "ClosestToStartSite version %s" % self._toolVersion
        epilog = "\nParser a bed file and create a bed file to create a report about positions of features A to features B. \n"
        epilog +="it can also add the class code of features A.\n"
        epilog += "example: ClosestToStartSite.py -i <inputFile> -c <cuff_in.tmap> -o <outputFile>\n"
        parser = RepetOptionParser(description = description, epilog = epilog, version = self._toolVersion) 
        parser.add_option("-i", "--inputFile",  dest = "inputFile",  action = "store", type = "string", help = "input bed file name.",  default = "")
        parser.add_option("-c", "--cuffcom_tmap",  dest = "cuffcom_tmap",  action = "store", type = "string", help = "input gtf file of cuffcompare (<cuff_in>.tmap)",  default = "")
        parser.add_option("-o", "--outputFile", dest = "outputFile", action = "store", type = "string", help = "output file name", default = "")
        parser.add_option("-v", "--verbosity",  dest = "verbosity",  action = "store", type = "int",    help = "verbosity [optional] [default: 3]",default = 3)
        options = parser.parse_args()[0]
        self._setAttributesFromOptions(options)

    def _setAttributesFromOptions(self, options):
        self._inputFile  = options.inputFile
        self._cuffcom_tmap = options.cuffcom_tmap
        self._outputFile = options.outputFile
        self._verbosity  = options.verbosity
                    
    def _logAndRaise(self, errorMsg):
        self._log.error(errorMsg)
        raise RepetException(errorMsg)

    def checkoption(self):
        if self._inputFile == "":
            self._log.info("Missing input file")
        else:
            if not FileUtils.isRessourceExists(self._inputFile):
                self._log.info("'%s' doesn't exist!" % self._inputFile)	

        if self._cuffcom_tmap != "":
            if not FileUtils.isRessourceExists(self._cuffcom_tmap):
                self._log.info("'%s' doesn't exist!" % self._cuffcom_tmap)
	    if self._outputFile == "":
		self._outputFile = "%s_Close2TSS_with_classcode.bed" % os.path.splitext(self._inputFile)[0]
    	    else:
           	if FileUtils.isRessourceExists(self._outputFile):
                	self._log.info("Output file '%s' already exists!" % self._outputFile)
        else:
           if self._outputFile == "":
                self._outputFile = "%s_Close2TSS.bed" % os.path.splitext(self._inputFile)[0]
           else:
           	if FileUtils.isRessourceExists(self._outputFile):
                	self._log.info("Output file '%s' already exists!" % self._outputFile)
		
            
    def getClassCodeCuffcompare(self, tmap_file, listPossitions):
        class_code_dic = {}
        lcode_ref = []
        tmp = []
        linetowrite =[]
        with open(tmap_file) as tmap:
                tmapline = tmap.readlines()
                for i in range(1,len(tmapline)):
                    cuff_id = tmapline[i].split("\t")[4].strip()
                    class_code = tmapline[i].split("\t")[2].strip()
                    ref_id = tmapline[i].split("\t")[1].strip()
                    lcode_ref.append(class_code)
                    lcode_ref.append(ref_id)
                    class_code_dic[cuff_id] = lcode_ref
                    lcode_ref = []
        
        
        for i in xrange(0,len(listPossitions)):
            tmp.extend(listPossitions[i])
            transcript_bedtools = listPossitions[i][3]
                
            if transcript_bedtools in class_code_dic.keys():
                    tmp.append(class_code_dic[transcript_bedtools][0])
                    tmp.append(class_code_dic[transcript_bedtools][1])
            else:
                    tmp.append("NA")
                    tmp.append("NA")
            linetowrite.append(tmp)
            tmp=[]
        return linetowrite
        

           
    def getClosestToStartSite(self, inputFile):
        linelist = []
        tmplist = []
        with open(inputFile, "r") as bedFile:
            for line in bedFile.readlines():
                m = re.search(r"^\s*(\S+)\t+(\d+)\t+(\d+)\t+([^\t]+)\t+([^\t]+)\t+([+-])\t+(\d+\.\d+)\t+([^\t]+)+\t+(\d+)\t+(\d+)\t+([^\t]+)+\t+([^\t]+)\t+([+-])\t+([^\t]+)",line)
                if(m != None):
                    start_TR = int(m.group(2))##F[1]
                    end_TR = int(m.group(3))##F[2]
                    strand_TR= m.group(6) ##[5]
                    start_TE = int(m.group(9))##[8]
                    end_TE = int(m.group(10))##[9]
                    dist = int(m.group(14))##[13]
                    if (start_TE < start_TR) and (end_TE < start_TR) and (strand_TR =="+") and (end_TR > end_TE) and (end_TR > start_TE) and (dist != 0):
                        for i in range(0,len(line.split("\t"))-1):
                            tmplist.append(line.split("\t")[i])
                        tmplist.append(line.split("\t")[13].strip())
                        tmplist.append("TE_closest_TSS")
                        linelist.append(tmplist)
                        tmplist = []
            #                        F[1]   gene             F[2]
            #                        =========================>
            #         ------------
            #        F[8]        F[9]
    
                    if (start_TE > end_TR) and (end_TE > end_TR) and (strand_TR =="-") and (start_TR < start_TE) and (start_TR < end_TE) and (dist != 0):
                        for i in range(0,len(line.split("\t"))-1):
                            tmplist.append(line.split("\t")[i])
                        tmplist.append(line.split("\t")[13].strip())
                        tmplist.append("TE_closest_TSS")
                        linelist.append(tmplist)
                        tmplist = []
            
            #                        F[1]                F[2]
            #                        <======================
            #                                                    ---------------
                  
                    if (start_TE <= start_TR) and (start_TR < end_TE) and (strand_TR =="+") and (end_TR > end_TE) and (end_TR > start_TE):
                        for i in range(0,len(line.split("\t"))-1):
                            tmplist.append(line.split("\t")[i])
                        overlap = (end_TE-start_TR)+1
                        tmplist.append(overlap)
                        tmplist.append("TE_overlap_TSS")
                        linelist.append(tmplist)
                        tmplist = []
            
            #                        F[1]   gene             F[2]
            #                        =========================>
            #                 -------------
            #                F[8]        F[9]
            
            #                                         gene
            #                                F[1]=========================>F[2]
            
            #                                F[8]---------------F[9]
            
            
                    if (start_TE < start_TR) and (start_TR == end_TE) and (strand_TR =="+") and (end_TR > end_TE) and (end_TR > start_TE):
                        for i in range(0,len(line.split("\t"))-1):
                            tmplist.append(line.split("\t")[i])
                        tmplist.append(0)
                        tmplist.append("TE_overlap_TSS")
                        linelist.append(tmplist)
                        tmplist = []
            
            ##                                F[1]=============================>F[2]
            ##                 F[8]---------------F[9]
            
            
                    if (start_TE < end_TR) and (end_TR <= end_TE) and (strand_TR =="-") and (start_TR < start_TE) and (start_TR < end_TE):
                        for i in range(0,len(line.split("\t"))-1):
                            tmplist.append(line.split("\t")[i])
                        overlap = (end_TR-start_TE)+1
                        tmplist.append(overlap)
                        tmplist.append("TE_overlap_TSS")
                        linelist.append(tmplist)
                        tmplist = []
            
            
            #                        F[1]<======================F[2]
            #                                           ---------------
            #                                        F[8]           F[9]
            #
            #
            #                    F[1]<=============================F[2]
            #                                   F[8]---------------F[9]
            
                    if (start_TE == end_TR) and (end_TR < end_TE) and (strand_TR =="-") and (start_TR < start_TE) and (start_TR < end_TE):
                        for i in range(0,len(line.split("\t"))-1):
                            tmplist.append(line.split("\t")[i])
                        tmplist.append(0)
                        tmplist.append("TE_overlap_TSS")
                        linelist.append(tmplist)
                        tmplist = []
            
            #                                                F[1]<=============================F[2]
            #                                                                                F[8]---------------F[9]
            
                    if (start_TR < start_TE) and (start_TR < end_TE) and (start_TE < end_TR) and (end_TE < end_TR) and (dist == 0):
                        for i in range(0,len(line.split("\t"))-1):
                            tmplist.append(line.split("\t")[i])
                        tmplist.append(0)
                        #tmplist.append(line.strip())
                        tmplist.append("TE_in_gene")
                        linelist.append(tmplist)
                        tmplist = []
                  
            
            #                        F[1]            gene          F[2]
            #                        ==============================
            #                                 -----------
            #                                F[8]        F[9]
            
            
                    if (start_TE < start_TR) and (start_TR < end_TE) and (start_TE < end_TR) and (end_TR < end_TE):
                        for i in range(0,len(line.split("\t"))-1):
                            tmplist.append(line.split("\t")[i])
                        #lenTE = (end_TE-start_TE)+1
                        tmplist.append(0)
                        tmplist.append("gene_in_TE")
                        linelist.append(tmplist)
                        tmplist = []
                        
            #                        F[1]======================F[2]
            #        F[8]----------------------------------------------------F[9]
            
            
                    if (strand_TR =="+") and (start_TR > start_TE) and (start_TR < end_TE) and (start_TE < end_TR) and (end_TE == end_TR):
                        for i in range(0,len(line.split("\t"))-1):
                            tmplist.append(line.split("\t")[i])
                        tmplist.append(0)
                        #tmplist.append(line.strip())
                        tmplist.append("gene_in_TE")
                        linelist.append(tmplist)
                        tmplist = []
            
            #                                                                F[1]==================================>F[2]
            #                                        F[8]----------------------------------------------------------F[9]
            
                    if (strand_TR =="-") and (start_TR > start_TE) and (start_TR < end_TE) and (start_TE < end_TR) and (end_TE == end_TR):
                        for i in range(0,len(line.split("\t"))-1):
                            tmplist.append(line.split("\t")[i])
                        tmplist.append(0)
                        #tmplist.append(line.strip())
                        tmplist.append("gene_in_TE")
                        linelist.append(tmplist)
                        tmplist = []
            
            #                                                                F[1]<==================================F[2]
            #                                        F[8]----------------------------------------------------------F[9]
            
                    if (strand_TR =="+") and (start_TR == start_TE) and (start_TR < end_TE) and (start_TE < end_TR) and (end_TE > end_TR):
                        for i in range(0,len(line.split("\t"))-1):
                            tmplist.append(line.split("\t")[i])
                        tmplist.append(0)
                        #tmplist.append(line.strip())
                        tmplist.append("gene_in_TE")
                        linelist.append(tmplist)
                        tmplist = []
            
            #                                                                F[1]==================================>F[2]
            #                                                                F[8]----------------------------------------------------------F[9]
            
                    if (strand_TR =="-") and (start_TR == start_TE) and (start_TR < end_TE) and (start_TE < end_TR) and (end_TE > end_TR):
                        for i in range(0,len(line.split("\t"))-1):
                            tmplist.append(line.split("\t")[i])
                        tmplist.append(0)
                        #tmplist.append(line.strip())
                        tmplist.append("gene_in_TE")
                        linelist.append(tmplist)
                        tmplist = []
            
            #                                                                F[1]<==================================F[2]
            #                                                                F[8]----------------------------------------------------------F[9]
        
        return linelist
    
    
    def writeOutputFromList(self, listPossitions , outputFile):
        w = open(outputFile,'w')
        for s in listPossitions:
            line= "\t".join(str(item) for item in s)
            w.write("%s\n" % line)
        w.close()

                
    def run(self):
        self.checkoption()
        listPossitions = self.getClosestToStartSite(self._inputFile)
        if self._cuffcom_tmap == "":
            self.writeOutputFromList(listPossitions, self._outputFile )
        else:
            listclasscode = self.getClassCodeCuffcompare(self._cuffcom_tmap, listPossitions)
            self.writeOutputFromList(listclasscode, self._outputFile)
                
if __name__== "__main__":
    iClosestToStartSite = ClosestToStartSite()
    iClosestToStartSite.setAttributesFromCmdLine()
    iClosestToStartSite.run()