diff src/LIMMAscriptV4.R @ 0:4764dc6a1019 draft

"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
author vandelj
date Fri, 26 Jun 2020 09:51:15 -0400
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/src/LIMMAscriptV4.R	Fri Jun 26 09:51:15 2020 -0400
@@ -0,0 +1,1002 @@
+# A command-line interface for LIMMA to use with Galaxy
+# written by Jimmy Vandel
+# one of these arguments is required:
+#
+#
+initial.options <- commandArgs(trailingOnly = FALSE)
+file.arg.name <- "--file="
+script.name <- sub(file.arg.name, "", initial.options[grep(file.arg.name, initial.options)])
+script.basename <- dirname(script.name)
+source(file.path(script.basename, "utils.R"))
+source(file.path(script.basename, "getopt.R"))
+
+#addComment("Welcome R!")
+
+# setup R error handling to go to stderr
+options( show.error.messages=F, error = function () { cat(geterrmessage(), file=stderr() ); q( "no", 1, F ) } )
+
+# we need that to not crash galaxy with an UTF8 error on German LC settings.
+loc <- Sys.setlocale("LC_MESSAGES", "en_US.UTF-8")
+loc <- Sys.setlocale("LC_NUMERIC", "C")
+
+#get starting time
+start.time <- Sys.time()
+
+options(stringAsfactors = FALSE, useFancyQuotes = FALSE)
+args <- commandArgs()
+
+# get options, using the spec as defined by the enclosed list.
+# we read the options from the default: commandArgs(TRUE).
+spec <- matrix(c(
+  "dataFile", "i", 1, "character",
+  "factorInfo","a", 1, "character",
+  "blockingInfo","b", 1, "character",
+  "dicoRenaming","g",1,"character",
+  "blockingPolicy","u", 1, "character",
+  "fdrThreshold","t", 1, "double",
+  "thresholdFC","d", 1, "double",
+  "format", "f", 1, "character",
+  "histo","h", 1, "character",
+  "volcano","v", 1, "character",
+  "factorsContrast","r", 1, "character",
+  "contrastNames","p", 1, "character",
+  "firstGroupContrast","m", 1, "character",
+  "secondGroupContrast","n", 1, "character",
+  "controlGroups","c", 1, "character",
+  "fratioFile","s",1,"character",
+  "organismID","x",1,"character",
+  "rowNameType","y",1,"character",
+  "quiet", "q", 0, "logical",
+  "log", "l", 1, "character",
+  "outputFile" , "o", 1, "character",
+  "outputDfFile" , "z", 1, "character"),
+  byrow=TRUE, ncol=4)
+opt <- getopt(spec)
+
+# enforce the following required arguments
+if (is.null(opt$log)) {
+  addComment("[ERROR]'log file' is required\n")
+  q( "no", 1, F )
+}
+addComment("[INFO]Start of R script",T,opt$log,display=FALSE)
+if (is.null(opt$dataFile)) {
+  addComment("[ERROR]'dataFile' is required",T,opt$log)
+  q( "no", 1, F )
+}
+if (!is.null(opt$blockingInfo) && is.null(opt$blockingPolicy) ) {
+  addComment("[ERROR]blocking policy is missing",T,opt$log)
+  q( "no", 1, F )
+}
+if (is.null(opt$dicoRenaming)) {
+  addComment("[ERROR]renaming dictionnary is missing",T,opt$log)
+  q( "no", 1, F )
+}
+if (is.null(opt$factorsContrast)) {
+  addComment("[ERROR]factor informations are missing",T,opt$log)
+  q( "no", 1, F )
+}
+if (length(opt$firstGroupContrast)!=length(opt$secondGroupContrast)) {
+  addComment("[ERROR]some contrast groups seems to be empty",T,opt$log)
+  q( "no", 1, F )
+}
+if (is.null(opt$factorInfo)) {
+  addComment("[ERROR]factors info is missing",T,opt$log)
+  q( "no", 1, F )
+}
+if (is.null(opt$format)) {
+  addComment("[ERROR]'output format' is required",T,opt$log)
+  q( "no", 1, F )
+}
+if (is.null(opt$fdrThreshold)) {
+  addComment("[ERROR]'FDR threshold' is required",T,opt$log)
+  q( "no", 1, F )
+}
+if (is.null(opt$outputFile) || is.null(opt$outputDfFile)){
+  addComment("[ERROR]'output files' are required",T,opt$log)
+  q( "no", 1, F )
+}
+if (is.null(opt$thresholdFC)){
+  addComment("[ERROR]'FC threshold' is required",T,opt$log)
+  q( "no", 1, F )
+}
+if (is.null(opt$fratioFile)) {
+  addComment("[ERROR]F-ratio parameter is missing",T,opt$log)
+  q( "no", 1, F )
+}
+
+#demande si le script sera bavard
+verbose <- if (is.null(opt$quiet)) {
+  TRUE
+}else{
+  FALSE
+}
+
+#paramètres internes
+#pour savoir si on remplace les FC calculés par LIMMA par un calcul du LS-MEAN (ie moyenne de moyennes de chaque groupe dans chaque terme du contraste plutôt qu'une moyenne globale dans chaque terme)
+useLSmean=FALSE
+
+addComment("[INFO]Parameters checked!",T,opt$log,display=FALSE)
+
+addComment(c("[INFO]Working directory: ",getwd()),TRUE,opt$log,display=FALSE)
+addComment(c("[INFO]Command line: ",args),TRUE,opt$log,display=FALSE)
+
+#directory for plots
+dir.create(file.path(getwd(), "plotDir"))
+dir.create(file.path(getwd(), "plotLyDir"))
+
+#charge des packages silencieusement
+suppressPackageStartupMessages({
+  library("methods")
+  library("limma")
+  library("biomaRt")
+  library("ggplot2")
+  library("plotly")
+  library("stringr")
+  library("RColorBrewer")
+})
+
+
+#chargement du fichier dictionnaire de renommage
+renamingDico=read.csv(file=file.path(getwd(), opt$dicoRenaming),header=F,sep="\t",colClasses="character")
+rownames(renamingDico)=renamingDico[,2]
+
+
+#chargement des fichiers en entrée
+expDataMatrix=read.csv(file=file.path(getwd(), opt$dataFile),header=F,sep="\t",colClasses="character")
+#remove first row to convert it as colnames (to avoid X before colnames with header=T)
+colNamesData=expDataMatrix[1,-1]
+expDataMatrix=expDataMatrix[-1,]
+#remove first colum to convert it as rownames
+rowNamesData=expDataMatrix[,1]
+expDataMatrix=expDataMatrix[,-1]
+if(is.data.frame(expDataMatrix)){
+  expDataMatrix=data.matrix(expDataMatrix)
+}else{
+  expDataMatrix=data.matrix(as.numeric(expDataMatrix))
+}
+dimnames(expDataMatrix)=list(rowNamesData,colNamesData)
+
+#test the number of rows that are constant in dataMatrix
+nbConstantRows=length(which(unlist(apply(expDataMatrix,1,var))==0))
+if(nbConstantRows>0){
+  addComment(c("[WARNING]",nbConstantRows,"rows are constant across conditions in input data file"),T,opt$log,display=FALSE)
+}
+
+#test if all condition names are present in dico
+if(!all(colnames(expDataMatrix) %in% rownames(renamingDico))){
+  addComment("[ERROR]Missing condition names in renaming dictionary",T,opt$log)
+  q( "no", 1, F )
+}
+
+addComment("[INFO]Expression data loaded and checked",T,opt$log,display=FALSE)
+addComment(c("[INFO]Dim of expression matrix:",dim(expDataMatrix)),T,opt$log,display=FALSE)
+
+#chargement du fichier des facteurs
+factorInfoMatrix=read.csv(file=file.path(getwd(), opt$factorInfo),header=F,sep="\t",colClasses="character")
+#remove first row to convert it as colnames
+colnames(factorInfoMatrix)=factorInfoMatrix[1,]
+factorInfoMatrix=factorInfoMatrix[-1,]
+#use first colum to convert it as rownames but not removing it to avoid conversion as vector in unique factor case
+rownames(factorInfoMatrix)=factorInfoMatrix[,1]
+
+if(length(setdiff(colnames(expDataMatrix),rownames(factorInfoMatrix)))!=0){
+  addComment("[ERROR]Missing samples in factor file",T,opt$log)
+  q( "no", 1, F )
+}
+
+#order sample as in expression matrix and remove spurious sample
+factorInfoMatrix=factorInfoMatrix[colnames(expDataMatrix),]
+
+#test if all values names are present in dico
+if(!all(unlist(factorInfoMatrix) %in% rownames(renamingDico))){
+  addComment("[ERROR]Missing factor names in renaming dictionary",T,opt$log)
+  q( "no", 1, F )
+}
+
+addComment("[INFO]Factors OK",T,opt$log,display=FALSE)
+addComment(c("[INFO]Dim of factorInfo matrix:",dim(factorInfoMatrix)),T,opt$log,display=FALSE)
+  
+##manage blocking factor
+blockingFactor=NULL
+blockinFactorsList=NULL
+if(!is.null(opt$blockingInfo)){
+  
+  #chargement du fichier des blocking factors
+  blockingInfoMatrix=read.csv(file=file.path(getwd(), opt$blockingInfo),header=F,sep="\t",colClasses="character")
+  #remove first row to convert it as colnames
+  colnames(blockingInfoMatrix)=blockingInfoMatrix[1,]
+  blockingInfoMatrix=blockingInfoMatrix[-1,]
+  #use first colum to convert it as rownames but not removing it to avoid conversion as vector in unique factor case
+  rownames(blockingInfoMatrix)=blockingInfoMatrix[,1]
+  
+  
+  if(length(setdiff(colnames(expDataMatrix),rownames(blockingInfoMatrix)))!=0){
+    addComment("[ERROR]Missing samples in blocking factor file",T,opt$log)
+    q( "no", 1, F )
+  }
+  
+  #order sample as in expression matrix
+  blockingInfoMatrix=blockingInfoMatrix[colnames(expDataMatrix),]
+  
+  #test if all blocking names are present in dico
+  if(!all(unlist(blockingInfoMatrix) %in% rownames(renamingDico))){
+    addComment("[ERROR]Missing blocking names in renaming dictionary",T,opt$log)
+    q( "no", 1, F )
+  }
+  
+  #remove blocking factors allready present as real factors
+  blockingNotInMainFactors=setdiff(colnames(blockingInfoMatrix)[-1],colnames(factorInfoMatrix)[-1])
+  
+  if(length(blockingNotInMainFactors)<(ncol(blockingInfoMatrix)-1))addComment("[WARNING]Blocking factors cannot be principal factors",T,opt$log,display=FALSE)
+  
+  if(length(blockingNotInMainFactors)>0){
+    
+    blockingInfoMatrix=blockingInfoMatrix[,c(colnames(blockingInfoMatrix)[1],blockingNotInMainFactors)]
+    
+    groupBlocking=rep("c",ncol(expDataMatrix))
+    #for each blocking factor
+    for(blockingFact in blockingNotInMainFactors){
+      if(opt$blockingPolicy=="correlated"){
+        indNewFact=as.numeric(factor(blockingInfoMatrix[,blockingFact]))
+        groupBlocking=paste(groupBlocking,indNewFact,sep="_")
+      }else{
+        if(is.null(blockinFactorsList))blockinFactorsList=list()
+        blockinFactorsList[[blockingFact]]=factor(unlist(lapply(blockingInfoMatrix[,blockingFact],function(x)paste(c(blockingFact,"_",x),collapse=""))))
+      }
+    }
+    if(opt$blockingPolicy=="correlated"){
+      blockingFactor=factor(groupBlocking)
+      if(length(levels(blockingFactor))==1){
+        addComment("[ERROR]Selected blocking factors seems to be constant",T,opt$log)
+        q( "no", 1, F )
+      }
+    }
+    addComment("[INFO]Blocking info OK",T,opt$log,display=FALSE)
+  }else{
+    addComment("[WARNING]No blocking factors will be considered",T,opt$log,display=FALSE)
+  }
+}
+
+
+##rename different input parameters using renamingDictionary
+opt$factorsContrast=renamingDico[unlist(lapply(unlist(strsplit(opt$factorsContrast,",")),function(x)which(renamingDico[,1]==x))),2]
+
+userDefinedContrasts=FALSE
+if(!is.null(opt$firstGroupContrast) && !is.null(opt$secondGroupContrast)){
+  userDefinedContrasts=TRUE
+  for(iContrast in 1:length(opt$firstGroupContrast)){
+    opt$firstGroupContrast[iContrast]=paste(unlist(lapply(unlist(strsplit(opt$firstGroupContrast[iContrast],",")),function(x)paste(renamingDico[unlist(lapply(unlist(strsplit(x,"\\*")),function(x)which(renamingDico[,1]==x))),2],collapse="*"))),collapse=",")
+    opt$secondGroupContrast[iContrast]=paste(unlist(lapply(unlist(strsplit(opt$secondGroupContrast[iContrast],",")),function(x)paste(renamingDico[unlist(lapply(unlist(strsplit(x,"\\*")),function(x)which(renamingDico[,1]==x))),2],collapse="*"))),collapse=",")
+  }
+}
+
+if(!is.null(opt$controlGroups)){
+  renamedGroups=c()
+  for(iGroup in unlist(strsplit(opt$controlGroups,","))){
+    renamedControlGroup=paste(renamingDico[unlist(lapply(unlist(strsplit(iGroup,":")),function(x)which(renamingDico[,1]==x))),2],collapse=":")
+    if(length(renamedControlGroup)==0 || any(which(unlist(gregexpr(text = renamedControlGroup,pattern = ":"))==-1))){
+      addComment("[ERROR]Control groups for interaction seem to mismatch, please check them.",T,opt$log)
+      q( "no", 1, F )
+    }
+    renamedGroups=c(renamedGroups,renamedControlGroup)
+  }
+  opt$controlGroups=renamedGroups
+}
+addComment("[INFO]Contrast variables are renamed to avoid confusion",T,opt$log,display=FALSE)
+##renaming done
+
+#to convert factor as numeric value --> useless now ?
+#expDataMatrix=apply(expDataMatrix,c(1,2),function(x)as.numeric(paste(x)))
+
+#get factors info for LIMMA
+factorsList=list()
+for(iFactor in opt$factorsContrast){
+  if(!(iFactor %in% colnames(factorInfoMatrix))){
+    addComment("[ERROR]Required factors are missing in input file",T,opt$log)
+    q( "no", 1, F )
+  }
+  factorsList[[iFactor]]=factor(unlist(lapply(factorInfoMatrix[,iFactor],function(x)paste(c(iFactor,"_",x),collapse=""))))
+  if(length(levels(factorsList[[iFactor]]))==1){
+    addComment("[ERROR]One selected factor seems to be constant",T,opt$log)
+    q( "no", 1, F )
+  }
+}
+
+#check if there is at least 2 factors to allow interaction computation
+if(!is.null(opt$controlGroups) && length(factorsList)<2){
+  addComment("[ERROR]You cannot ask for interaction with less than 2 factors",T,opt$log)
+  q( "no", 1, F )
+}
+
+#merge all factors as a single one
+factorsMerged=as.character(factorsList[[opt$factorsContrast[1]]])
+for(iFactor in opt$factorsContrast[-1]){
+  factorsMerged=paste(factorsMerged,as.character(factorsList[[iFactor]]),sep=".")
+}
+factorsMerged=factor(factorsMerged)
+
+#checked that coefficient number (ie. factorsMerged levels) is strictly smaller than sample size
+if(length(levels(factorsMerged))>=length(factorsMerged)){
+  addComment(c("[ERROR]No enough samples (",length(factorsMerged),") to estimate ",length(levels(factorsMerged))," coefficients"),T,opt$log)
+  q( "no", 1, F )
+}
+
+#get the sample size of each factor values
+sampleSizeFactor=table(factorsMerged)
+
+
+if(!is.null(blockinFactorsList)){
+  factorString=c("blockinFactorsList[['", names(blockinFactorsList)[1],"']]")
+  for(blockingFact in names(blockinFactorsList)[-1]){
+    factorString=c(factorString," + blockinFactorsList[['",blockingFact,"']]")
+  }
+  design = model.matrix(as.formula(paste(c("~ factorsMerged +",factorString," + 0"),collapse="")))
+  
+  #rename design columns
+  coeffMeaning = levels(factorsMerged)
+  for(blockingFact in blockinFactorsList){
+    coeffMeaning=c(coeffMeaning,levels(blockingFact)[-1])
+  }
+  colnames(design) = coeffMeaning
+}else{
+  design = model.matrix(as.formula( ~ factorsMerged + 0))
+  
+  #rename degin columns
+  coeffMeaning = levels(factorsMerged)
+  colnames(design) = coeffMeaning
+}
+  
+addComment(c("[INFO]Available coefficients: ",coeffMeaning),T,opt$log,display=F)
+
+estimableCoeff=which(colSums(design)!=0)
+  
+addComment("[INFO]Design done",T,opt$log,display=F)
+  
+  #use blocking factor if exists
+if(!is.null(blockingFactor)){
+  corfit <- duplicateCorrelation(expDataMatrix, design, block=blockingFactor)
+  
+  addComment(c("[INFO]Correlation within groups: ",corfit$consensus.correlation),T,opt$log,display=F)
+    
+  #run linear model  fit
+  data.fit = lmFit(expDataMatrix,design,block = blockingFactor, correlation=corfit$consensus.correlation)
+}else{
+  #run linear model  fit  
+  data.fit = lmFit(expDataMatrix,design)
+}
+  
+estimatedCoeff=which(!is.na(data.fit$coefficients[1,]))
+  
+addComment("[INFO]Lmfit done",T,opt$log,display=F)
+
+#catch situation where some coefficients cannot be estimated, probably due to dependances between design columns 
+#if(length(setdiff(estimableCoeff,estimatedCoeff))>0){
+#  addComment("[ERROR]Error in design matrix, check your group definitions",T,opt$log)
+#  q( "no", 1, F )
+#}
+#to strong condition, should return ERROR only when coefficients relative to principal factors cannot be estimated, otherwise, return a simple WARNING
+  
+#define requested contrasts 
+requiredContrasts=c()
+humanReadingContrasts=c()
+persoContrastName=c()
+if(userDefinedContrasts){
+  for(iContrast in 1:length(opt$firstGroupContrast)){
+    posGroup=unlist(lapply(unlist(strsplit(opt$firstGroupContrast[iContrast],",")),function(x)paste(paste(opt$factorsContrast,unlist(strsplit(x,"\\*")),sep="_"),collapse=".")))
+    negGroup=unlist(lapply(unlist(strsplit(opt$secondGroupContrast[iContrast],",")),function(x)paste(paste(opt$factorsContrast,unlist(strsplit(x,"\\*")),sep="_"),collapse=".")))
+    #clear posGroup and negGroup from empty groups
+    emptyPosGroups=which(!(posGroup%in%coeffMeaning))
+    if(length(emptyPosGroups)>0){
+      addComment(c("[WARNING]The group(s)",posGroup[emptyPosGroups],"is/are removed from contrast as it/they is/are empty"),T,opt$log,display=FALSE)
+      posGroup=posGroup[-emptyPosGroups]
+      currentHumanContrast=paste(unlist(strsplit(opt$firstGroupContrast[iContrast],","))[-emptyPosGroups],collapse="+") 
+    }else{
+      currentHumanContrast=paste(unlist(strsplit(opt$firstGroupContrast[iContrast],",")),collapse="+")  
+    }
+    emptyNegGroups=which(!(negGroup%in%coeffMeaning))
+    if(length(emptyNegGroups)>0){
+      addComment(c("[WARNING]The group(s)",negGroup[emptyNegGroups],"is/are removed from contrast as it/they is/are empty"),T,opt$log,display=FALSE)
+      negGroup=negGroup[-emptyNegGroups]
+      currentHumanContrast=paste(c(currentHumanContrast,unlist(strsplit(opt$secondGroupContrast[iContrast],","))[-emptyNegGroups]),collapse="-")
+    }else{
+      currentHumanContrast=paste(c(currentHumanContrast,unlist(strsplit(opt$secondGroupContrast[iContrast],","))),collapse="-")
+    }
+    if(length(posGroup)==0 || length(negGroup)==0 ){
+      addComment(c("[WARNING]Contrast",currentHumanContrast,"cannot be estimated due to empty group"),T,opt$log,display=FALSE)
+    }else{
+      if(all(posGroup%in%negGroup) && all(negGroup%in%posGroup)){
+        addComment(c("[WARNING]Contrast",currentHumanContrast,"cannot be estimated due to null contrast"),T,opt$log,display=FALSE)
+      }else{
+        #get coefficients required for first group added as positive
+        positiveCoeffWeights=sampleSizeFactor[posGroup]/sum(sampleSizeFactor[posGroup])
+        #positiveCoeffWeights=rep(1,length(posGroup))
+        #names(positiveCoeffWeights)=names(sampleSizeFactor[posGroup])
+        #get coefficients required for second group added as negative
+        negativeCoeffWeights=sampleSizeFactor[negGroup]/sum(sampleSizeFactor[negGroup])
+        #negativeCoeffWeights=rep(1,length(negGroup))
+        #names(negativeCoeffWeights)=names(sampleSizeFactor[negGroup])
+        #build the resulting contrast
+        currentContrast=paste(paste(positiveCoeffWeights[posGroup],posGroup,sep="*"),collapse="+")
+        currentContrast=paste(c(currentContrast,paste(paste(negativeCoeffWeights[negGroup],negGroup,sep="*"),collapse="-")),collapse="-")
+        requiredContrasts=c(requiredContrasts,currentContrast)
+        
+        #build the human reading contrast
+        humanReadingContrasts=c(humanReadingContrasts,currentHumanContrast)
+        if(!is.null(opt$contrastNames) && nchar(opt$contrastNames[iContrast])>0){
+          persoContrastName=c(persoContrastName,opt$contrastNames[iContrast])
+        }else{
+          persoContrastName=c(persoContrastName,"")
+        }
+        
+        addComment(c("[INFO]Contrast added : ",currentHumanContrast),T,opt$log,display=F)
+        addComment(c("with complete formula ",currentContrast),T,opt$log,display=F)
+      }
+    }
+  }
+}
+  
+  
+  #define the true formula with interactions to get interaction coefficients
+  factorString=c("factorsList[['", names(factorsList)[1],"']]")
+  for(iFactor in names(factorsList)[-1]){
+    factorString=c(factorString," * factorsList[['",iFactor,"']]")
+  }
+  
+  if(!is.null(blockinFactorsList)){
+    for(blockingFact in names(blockinFactorsList)){
+      factorString=c(factorString," + blockinFactorsList[['",blockingFact,"']]")
+    }
+  }
+  
+  #should not be null at the end 
+  allFtestMeanSquare=NULL
+  #to get the F-test values
+  estimatedInteractions=rownames(anova(lm(as.formula(paste(c("expDataMatrix[1,] ~ ",factorString),collapse="")))))
+  estimatedInteractions=c(unlist(lapply(estimatedInteractions[-length(estimatedInteractions)],function(x){temp=unlist(strsplit(x,"[ \" | : ]"));paste(temp[seq(2,length(temp),3)],collapse=":")})),estimatedInteractions[length(estimatedInteractions)])
+  #rename estimated interaction terms using renamingDico
+  estimatedInteractions=c(unlist(lapply(estimatedInteractions[-length(estimatedInteractions)],function(x)paste(renamingDico[unlist(strsplit(x,":")),1],collapse=":"))),estimatedInteractions[length(estimatedInteractions)])
+  t <- unlist(apply(expDataMatrix,1,function(x){temp=anova(lm(as.formula(paste(c("x ~ ",factorString),collapse=""))))$`Mean Sq`;temp/temp[length(temp)]}))
+  allFtestMeanSquare <- t(matrix(t,nrow=length(estimatedInteractions)))
+  #remove from allFtest rows containing NA
+  if(length(which(is.na(allFtestMeanSquare[,1])))>0)allFtestMeanSquare=allFtestMeanSquare[-(which(is.na(allFtestMeanSquare[,1]))),]
+  colnames(allFtestMeanSquare)=estimatedInteractions
+  
+  #add contrasts corresponding to interaction terms
+  if(!is.null(opt$controlGroups)){
+    #first load user defined control group for each factor
+    controlGroup=rep(NA,length(factorsList))
+    names(controlGroup)=names(factorsList)
+    for(iGroup in opt$controlGroups){
+      splitGroup=unlist(strsplit(iGroup,":"))
+      splitGroup[2]=paste(splitGroup[1],splitGroup[2],sep = "_")
+      #check if defined control group is really a level of the corresponding factor
+      if(!splitGroup[1]%in%names(controlGroup) || !splitGroup[2]%in%factorsList[[splitGroup[1]]]){
+        addComment(c("[ERROR]The factor name",splitGroup[1],"does not exist or group name",splitGroup[2]),T,opt$log)
+        q( "no", 1, F )
+      }
+      if(!is.na(controlGroup[splitGroup[1]])){
+        addComment("[ERROR]Several control groups are defined for the same factor, please select only one control group for each factor if you want to compute interaction contrasts",T,opt$log)
+        q( "no", 1, F )
+      }
+      controlGroup[splitGroup[1]]=splitGroup[2]
+    }
+    
+    #check if all factor have a defined control group
+    if(any(is.na(controlGroup))){
+      addComment("[ERROR]Missing control group for some factors, please check them if you want to compute interaction contrasts",T,opt$log)
+      q( "no", 1, F )
+    }
+    
+    nbFactors=length(factorsList)
+    interactionContrasts=c()
+    contrastClass=c()
+    #initialize list for the first level
+    newPreviousLoopContrast=list()
+    for(iFactorA in 1:(nbFactors-1)){
+      nameFactorA=names(factorsList)[iFactorA]
+      compA=c()
+      for(levelA in setdiff(levels(factorsList[[iFactorA]]),controlGroup[nameFactorA])){
+        compA=c(compA,paste(levelA,controlGroup[nameFactorA],sep="-"))
+      }
+      newPreviousLoopContrast[[as.character(iFactorA)]]=compA
+    }
+    #make a loop for growing interaction set
+    for(globalIfactor in 1:(nbFactors-1)){
+      previousLoopContrast=newPreviousLoopContrast
+      newPreviousLoopContrast=list()
+      #factor A reuse contrasts made at previsous loop
+      for(iFactorA in names(previousLoopContrast)){
+        compA=previousLoopContrast[[iFactorA]]
+  
+        if(max(as.integer(unlist(strsplit(iFactorA,"\\."))))<nbFactors){
+          #factor B is the new factor to include in intreraction set
+          for(iFactorB in (max(as.integer(unlist(strsplit(iFactorA,"\\."))))+1):nbFactors){
+            nameFactorB=names(factorsList)[iFactorB]
+            #keep contrasts identified trough interacting factors set
+            newPreviousLoopContrast[[paste(iFactorA,iFactorB,sep=".")]]=c()
+              for(iCompA in compA){
+                for(levelB in setdiff(levels(factorsList[[iFactorB]]),controlGroup[nameFactorB])){
+                  #decompose the contrast compA to apply the new level of factor B on each term
+                  temp=unlist(strsplit(iCompA,"[ + ]"))
+                  splitCompA=temp[1]
+                  for(iTemp in temp[-1])splitCompA=c(splitCompA,"+",iTemp)
+                  splitCompA=unlist(lapply(splitCompA,function(x){temp=unlist(strsplit(x,"-"));splitCompB=temp[1];for(iTemp in temp[-1])splitCompB=c(splitCompB,"-",iTemp);splitCompB}))
+                  #apply on each contrast term the new level of factor B
+                  firstTerm=paste(unlist(lapply(splitCompA,function(x)if(x!="+" && x!="-"){paste(x,levelB,sep=".")}else{x})),collapse="")
+                  secondTerm=negativeExpression(paste(unlist(lapply(splitCompA,function(x)if(x!="+" && x!="-"){paste(x,controlGroup[nameFactorB],sep=".")}else{x})),collapse=""))
+                  currentContrast=paste(c(firstTerm,secondTerm),collapse="")
+                  
+                  newPreviousLoopContrast[[paste(iFactorA,iFactorB,sep=".")]]=c(newPreviousLoopContrast[[paste(iFactorA,iFactorB,sep=".")]],currentContrast)
+                }
+              }
+            }
+        }
+      }
+      for(iContrast in names(newPreviousLoopContrast)){
+        contrastClass=c(contrastClass,rep(iContrast,length(newPreviousLoopContrast[[iContrast]])))
+      }
+      interactionContrasts=c(interactionContrasts,unlist(newPreviousLoopContrast))
+    }
+    #make human title for interactions
+    names(interactionContrasts)=contrastClass
+    humanReadingInteraction=unlist(lapply(interactionContrasts,function(x)gsub("\\.",":",unlist(strsplit(x,"[+-]"))[1])))
+    
+    contrastToIgnore=c()
+    
+    #complete with control groups and order to match to coeffs
+    for(iContrast in 1:length(interactionContrasts)){
+      missingFactor=setdiff(1:nbFactors,as.integer(unlist(strsplit(names(interactionContrasts[iContrast]),"\\."))))
+      #decompose the contrast
+      temp=unlist(strsplit(interactionContrasts[iContrast],"[ + ]"))
+      splitContrast=temp[1]
+      for(iTemp in temp[-1])splitContrast=c(splitContrast,"+",iTemp)
+      splitContrast=unlist(lapply(splitContrast,function(x){temp=unlist(strsplit(x,"-"));splitCompB=temp[1];for(iTemp in temp[-1])splitCompB=c(splitCompB,"-",iTemp);splitCompB}))
+      for(iFactor in missingFactor){
+        for(iTerm in 1:length(splitContrast)){
+          if(splitContrast[iTerm]!="+" && splitContrast[iTerm]!="-"){
+            splitTerm=unlist(strsplit(splitContrast[iTerm],"\\."))
+            if(iFactor==1)splitContrast[iTerm]=paste(c(controlGroup[names(factorsList)[iFactor]],splitTerm),collapse=".")
+            if(iFactor==nbFactors)splitContrast[iTerm]=paste(c(splitTerm,controlGroup[names(factorsList)[iFactor]]),collapse=".")
+            if(iFactor>1 && iFactor<nbFactors)splitContrast[iTerm]=paste(c(splitTerm[1:(iFactor-1)],controlGroup[names(factorsList)[iFactor]],splitTerm[iFactor:length(splitTerm)]),collapse=".")
+          }
+        }
+      }
+      interactionContrasts[iContrast]=paste(splitContrast,collapse="")
+      if(all(splitContrast[seq(1,length(splitContrast),2)]%in%coeffMeaning)){
+        addComment(c("[INFO]Interaction contrast added : ",humanReadingInteraction[iContrast]),T,opt$log,display=F)
+        addComment(c("with complete formula ",interactionContrasts[iContrast]),T,opt$log,display=F)
+      }else{
+        contrastToIgnore=c(contrastToIgnore,iContrast)
+        addComment(c("[WARNING]Interaction contrast",humanReadingInteraction[iContrast],"is removed due to empty group"),T,opt$log,display=F)
+      }
+    }
+    
+    #add interaction contrasts to global contrast list
+    if(length(contrastToIgnore)>0){
+      requiredContrasts=c(requiredContrasts,interactionContrasts[-contrastToIgnore])
+      humanReadingContrasts=c(humanReadingContrasts,humanReadingInteraction[-contrastToIgnore])
+      persoContrastName=c(persoContrastName,rep("",length(humanReadingInteraction[-contrastToIgnore])))
+    }else{
+      requiredContrasts=c(requiredContrasts,interactionContrasts)
+      humanReadingContrasts=c(humanReadingContrasts,humanReadingInteraction)
+      persoContrastName=c(persoContrastName,rep("",length(humanReadingInteraction)))
+    }
+  }#end of intreaction contrasts
+  
+  
+  #remove from requiredContrasts contrasts that cannot be estimated
+  toRemove=unique(unlist(lapply(setdiff(coeffMeaning,names(estimatedCoeff)),function(x)grep(x,requiredContrasts))))
+  if(length(toRemove)>0){
+    addComment(c("[WARNING]",length(toRemove)," contrasts are removed, due to missing coefficients"),T,opt$log,display=FALSE)
+    requiredContrasts=requiredContrasts[-toRemove]
+    humanReadingContrasts=humanReadingContrasts[-toRemove]
+    persoContrastName=persoContrastName[-toRemove]
+  }
+  
+  if(length(requiredContrasts)==0){
+    addComment("[ERROR]No contrast to compute, please check your contrast definition.",T,opt$log)
+    q( "no", 1, F )
+  }
+  
+  #compute for each contrast mean of coefficients in posGroup and negGroup for FC computation of log(FC) with LSmean as in Partek
+  meanPosGroup=list()
+  meanNegGroup=list()
+  for(iContrast in 1:length(requiredContrasts)){
+    #define posGroup and negGroup
+    #first split contrast 
+    temp=unlist(strsplit(requiredContrasts[iContrast],"[ + ]"))
+    splitContrast=temp[1]
+    for(iTemp in temp[-1])splitContrast=c(splitContrast,"+",iTemp)
+    splitContrast=unlist(lapply(splitContrast,function(x){temp=unlist(strsplit(x,"-"));splitCompB=temp[1];for(iTemp in temp[-1])splitCompB=c(splitCompB,"-",iTemp);splitCompB}))
+    #and then put each term in good group
+    posGroup=c()
+    negGroup=c()
+    nextIsPos=TRUE
+    for(iSplit in splitContrast){
+      if(iSplit=="+")nextIsPos=TRUE
+      if(iSplit=="-")nextIsPos=FALSE
+      if(iSplit!="-" && iSplit!="+"){
+        #remove weights of contrast terms
+        iSplitBis=unlist(strsplit(iSplit,"[*]"))
+        iSplitBis=iSplitBis[length(iSplitBis)]
+        if(nextIsPos)posGroup=c(posGroup,iSplitBis)
+        else negGroup=c(negGroup,iSplitBis)
+      }
+    }
+    #compute means for each group
+    meanPosGroup[[iContrast]]=apply(as.matrix(data.fit$coefficients[,posGroup],ncol=length(posGroup)),1,mean)
+    meanNegGroup[[iContrast]]=apply(as.matrix(data.fit$coefficients[,negGroup],ncol=length(negGroup)),1,mean)
+  }
+
+  
+  contrast.matrix = makeContrasts(contrasts=requiredContrasts,levels=design)
+  data.fit.con = contrasts.fit(data.fit,contrast.matrix)
+  
+  addComment("[INFO]Contrast definition done",T,opt$log,T,display=FALSE)
+  
+  #compute LIMMA statistics
+  data.fit.eb = eBayes(data.fit.con)
+  
+  addComment("[INFO]Estimation done",T,opt$log,T,display=FALSE)
+  
+  #adjust p.value through FDR
+  data.fit.eb$adj_p.value=data.fit.eb$p.value
+  for(iComparison in 1:ncol(data.fit.eb$adj_p.value)){
+    data.fit.eb$adj_p.value[,iComparison]=p.adjust(data.fit.eb$p.value[,iComparison],"fdr")
+  }
+
+  #add a new field based on LS-means for each contrast instead of global mean like they were calculated in coefficients field
+  data.fit.eb$coefficientsLS=data.fit.eb$coefficients
+  if(ncol(data.fit.eb$coefficients)!=length(meanPosGroup)){
+    addComment("[ERROR]Estimated contrasts number unexpected",T,opt$log)
+    q( "no", 1, F )
+  }
+  for(iContrast in 1:length(meanPosGroup)){
+    data.fit.eb$coefficientsLS[,iContrast]=meanPosGroup[[iContrast]][rownames(data.fit.eb$coefficientsLS)]-meanNegGroup[[iContrast]][rownames(data.fit.eb$coefficientsLS)]
+  }
+  
+  #if requested replace coefficient computed as global mean by LS-means values
+  if(useLSmean)data.fit.eb$coefficients=data.fit.eb$coefficientsLS
+
+addComment("[INFO]Core treatment done",T,opt$log,T,display=FALSE)
+  
+  
+##convert humanReadingContrasts with namingDictionary to create humanReadingContrastsRenamed and keep original humanReadingContrasts names for file names 
+humanReadingContrastsRenamed=rep("",length(humanReadingContrasts))
+for(iContrast in 1:length(humanReadingContrasts)){
+  if(persoContrastName[iContrast]==""){
+    #if(verbose)addComment(humanReadingContrasts[iContrast])
+    specialCharacters=str_extract_all(humanReadingContrasts[iContrast],"[+|*|_|:|-]")[[1]]
+    #if(verbose)addComment(specialCharacters)
+    nameConverted=unlist(lapply(strsplit(humanReadingContrasts[iContrast],"[+|*|_|:|-]")[[1]],function(x)renamingDico[x,1]))
+    #if(verbose)addComment(nameConverted)
+    humanReadingContrastsRenamed[iContrast]=paste(nameConverted,specialCharacters,collapse="",sep="")
+    #if(verbose)addComment(humanReadingContrastsRenamed[iContrast])
+    humanReadingContrastsRenamed[iContrast]=substr(humanReadingContrastsRenamed[iContrast],1,nchar(humanReadingContrastsRenamed[iContrast])-1)
+  }else{
+    humanReadingContrastsRenamed[iContrast]=persoContrastName[iContrast]
+  }
+}
+
+#write correspondances between plot file names (humanReadingContrasts) and displayed names in figure legends (humanReadingContrastsRenamed), usefull to define html items in xml file
+correspondanceTable=matrix("",ncol=2,nrow=ncol(data.fit.eb$p.value))
+correspondanceTable[,1]=unlist(lapply(humanReadingContrasts,function(x)gsub(":","_INT_",gsub("\\+","_PLUS_",gsub("\\*","_AND_",x)))))
+correspondanceTable[,2]=humanReadingContrastsRenamed
+rownames(correspondanceTable)=correspondanceTable[,2]
+write.table(correspondanceTable,file=file.path(getwd(), "correspondanceFileNames.csv"),quote=FALSE,sep="\t",col.names = F,row.names = F)
+
+#plot nominal p-val histograms for selected comparisons
+histogramPerPage=6
+if (!is.null(opt$histo)) {
+  iToPlot=1
+  plotVector=list()
+  nbComparisons=ncol(data.fit.eb$p.value)
+  for (iComparison in 1:nbComparisons){
+    dataToPlot=data.frame(pval=data.fit.eb$p.value[,iComparison],id=rownames(data.fit.eb$p.value))
+    p <- ggplot(data=dataToPlot, aes(x=pval)) + geom_histogram(colour="red", fill="salmon") +
+      theme_bw() + ggtitle(humanReadingContrastsRenamed[iComparison]) + ylab(label="Frequencies") + xlab(label="Nominal p-val") +
+      theme(panel.border=element_blank(),plot.title = element_text(hjust = 0.5))
+    plotVector[[length(plotVector)+1]]=p
+    
+    pp <- ggplotly(p)
+    htmlwidgets::saveWidget(as_widget(pp), paste(c(file.path(getwd(), "plotLyDir"),"/",opt$histo,"_",correspondanceTable[humanReadingContrastsRenamed[iComparison],1],".html"),collapse=""),selfcontained = F)
+    
+    if(iComparison==nbComparisons || length(plotVector)==histogramPerPage){
+      #plot and close the actual plot
+      if(opt$format=="pdf"){
+        pdf(paste(c("./plotDir/",opt$histo,iToPlot,".pdf"),collapse=""))}else{
+          png(paste(c("./plotDir/",opt$histo,iToPlot,".png"),collapse=""))
+        }
+      multiplot(plotlist=plotVector,cols=2)
+      dev.off()
+      if(iComparison<nbComparisons){
+        #prepare for a new plotting file if necessary
+        plotVector=list()
+        iToPlot=iToPlot+1
+      }
+    }
+  }
+  addComment("[INFO]Histograms drawn",T,opt$log,T,display=FALSE)
+  
+}
+
+#plot F-test sum square barplot
+if(!is.null(allFtestMeanSquare)){
+  dataToPlot=data.frame(Fratio=apply(allFtestMeanSquare,2,mean),Factors=factor(colnames(allFtestMeanSquare),levels = colnames(allFtestMeanSquare)))
+
+  p <- ggplot(data=dataToPlot, aes(x=Factors, y=Fratio, fill=Factors)) +
+    geom_bar(stat="identity") + scale_fill_manual(values = colorRampPalette(brewer.pal(9,"Set1"))(ncol(allFtestMeanSquare))[sample(ncol(allFtestMeanSquare))]) + ylab(label="mean F-ratio") +
+    theme_bw() + theme(panel.border=element_blank(),plot.title = element_text(hjust = 0.5)) + ggtitle("Source of variation")
+  
+   if(opt$format=="pdf"){
+    pdf(paste(c("./plotDir/",opt$fratioFile,".pdf"),collapse=""))}else{
+      png(paste(c("./plotDir/",opt$fratioFile,".png"),collapse=""))
+    }
+  plot(p)
+  dev.off()
+  
+  pp <- ggplotly(p)
+  htmlwidgets::saveWidget(as_widget(pp), paste(c(file.path(getwd(), "plotLyDir"),"/",opt$fratioFile,".html"),collapse=""),selfcontained = F)
+  
+  addComment("[INFO]SumSquareTest drawn",T,opt$log,T,display=FALSE)
+}
+
+#plot VOLCANO plot
+#volcanoplot(data.fit.eb,coef=1,highlight=10)
+volcanoPerPage=1
+logFCthreshold=log2(opt$thresholdFC)
+if (!is.null(opt$volcano)) {
+  iToPlot=1
+  plotVector=list()
+  nbComparisons=ncol(data.fit.eb$adj_p.value)
+  for (iComparison in 1:nbComparisons){
+    
+    #define the log10(p-val) threshold corresponding to FDR threshold fixed by user
+    probeWithLowFDR=-log10(data.fit.eb$p.value[which(data.fit.eb$adj_p.value[,iComparison]<=opt$fdrThreshold),iComparison])
+    pvalThresholdFDR=NULL
+    if(length(probeWithLowFDR)>0)pvalThresholdFDR=min(probeWithLowFDR)
+    
+    #get significant points over FC and FDR thresholds
+    significativePoints=intersect(which(abs(data.fit.eb$coefficients[,iComparison])>=logFCthreshold),which(data.fit.eb$adj_p.value[,iComparison]<=opt$fdrThreshold))
+    
+    #to reduce size of html plot, we keep 20000 points maximum sampled amongst genes with pval>=33%(pval) and abs(log2(FC))<=66%(abs(log2(FC)))
+    htmlPointsToRemove=intersect(which(abs(data.fit.eb$coefficients[,iComparison])<=quantile(abs(data.fit.eb$coefficients[,iComparison]),c(0.66))),which(data.fit.eb$p.value[,iComparison]>=quantile(abs(data.fit.eb$p.value[,iComparison]),c(0.33))))
+    if(length(htmlPointsToRemove)>20000){
+      htmlPointsToRemove=setdiff(htmlPointsToRemove,sample(htmlPointsToRemove,20000))
+    }else{
+      htmlPointsToRemove=c() 
+    }
+      
+      xMinLimPlot=min(data.fit.eb$coefficients[,iComparison])-0.2
+      xMaxLimPlot=max(data.fit.eb$coefficients[,iComparison])+0.2
+      yMaxLimPlot= max(-log10(data.fit.eb$p.value[,iComparison]))+0.2
+      
+      if(length(significativePoints)>0){
+        dataSignifToPlot=data.frame(pval=-log10(data.fit.eb$p.value[significativePoints,iComparison]),FC=data.fit.eb$coefficients[significativePoints,iComparison],description=paste(names(data.fit.eb$coefficients[significativePoints,iComparison]),"\n","FC: " , round(2^data.fit.eb$coefficients[significativePoints,iComparison],2) , " | FDR p-val: ",prettyNum(data.fit.eb$adj_p.value[significativePoints,iComparison],digits=4), sep=""))
+        #to test if remains any normal points to draw
+        if(length(significativePoints)<nrow(data.fit.eb$p.value)){
+          dataToPlot=data.frame(pval=-log10(data.fit.eb$p.value[-significativePoints,iComparison]),FC=data.fit.eb$coefficients[-significativePoints,iComparison],description=paste("FC: " , round(2^data.fit.eb$coefficients[-significativePoints,iComparison],2) , " | FDR p-val: ",prettyNum(data.fit.eb$adj_p.value[-significativePoints,iComparison],digits=4), sep=""))
+        }else{
+          dataToPlot=data.frame(pval=0,FC=0,description="null")
+        }
+      }else{
+        dataToPlot=data.frame(pval=-log10(data.fit.eb$p.value[,iComparison]),FC=data.fit.eb$coefficients[,iComparison],description=paste("FC: " , round(2^data.fit.eb$coefficients[,iComparison],2) , " | FDR p-val: ",prettyNum(data.fit.eb$adj_p.value[,iComparison],digits=4), sep=""))
+      }
+        
+      ##traditional plot
+      p <- ggplot(data=dataToPlot, aes(x=FC, y=pval)) + geom_point() + 
+        theme_bw() + ggtitle(humanReadingContrastsRenamed[iComparison]) + ylab(label="-log10(p-val)") + xlab(label="Log2 Fold Change") +
+        theme(panel.border=element_blank(),plot.title = element_text(hjust = 0.5),legend.position="none")
+      if(logFCthreshold!=0) p <- p + geom_vline(xintercept=-logFCthreshold, color="salmon",linetype="dotted", size=1) + geom_vline(xintercept=logFCthreshold, color="salmon",linetype="dotted", size=1) + geom_text(data.frame(text=c(paste(c("log2(1/FC=",opt$thresholdFC,")"),collapse=""),paste(c("log2(FC=",opt$thresholdFC,")"),collapse="")),x=c(-logFCthreshold,logFCthreshold),y=c(0,0)),mapping=aes(x=x, y=y, label=text), size=4, angle=90, vjust=-0.4, hjust=0, color="salmon")
+      if(!is.null(pvalThresholdFDR)) p <- p + geom_hline(yintercept=pvalThresholdFDR, color="skyblue1",linetype="dotted", size=0.5) + geom_text(data.frame(text=c(paste(c("FDR pval limit(",opt$fdrThreshold,")"),collapse="")),x=c(xMinLimPlot),y=c(pvalThresholdFDR)),mapping=aes(x=x, y=y, label=text), size=4, vjust=0, hjust=0, color="skyblue3")
+      if(length(significativePoints)>0)p <- p + geom_point(data=dataSignifToPlot,aes(colour=description))
+      
+      ##interactive plot
+      if(length(htmlPointsToRemove)>0){
+        pointToRemove=union(htmlPointsToRemove,significativePoints)
+        #to test if remains any normal points to draw
+        if(length(pointToRemove)<nrow(data.fit.eb$p.value)){
+          dataToPlot=data.frame(pval=-log10(data.fit.eb$p.value[-pointToRemove,iComparison]),FC=data.fit.eb$coefficients[-pointToRemove,iComparison],description=paste("FC: " , round(2^data.fit.eb$coefficients[-pointToRemove,iComparison],2) , " | FDR p-val: ", prettyNum(data.fit.eb$adj_p.value[-pointToRemove,iComparison],digits=4), sep=""))
+        }else{
+          dataToPlot=data.frame(pval=0,FC=0,description="null")
+        }
+      }
+      
+      if((nrow(dataToPlot)+nrow(dataSignifToPlot))>40000)addComment(c("[WARNING]For",humanReadingContrastsRenamed[iComparison],"volcano, numerous points to plot(",nrow(dataToPlot)+nrow(dataSignifToPlot),"), resulting volcano could be heavy, using more stringent thresholds could be helpful."),T,opt$log)
+      
+      phtml <- plot_ly(data=dataToPlot, x=~FC, y=~pval,type="scatter", mode="markers",showlegend = FALSE, marker = list(color="gray",opacity=0.5), text=~description, hoverinfo="text") %>%
+        layout(title = humanReadingContrastsRenamed[iComparison],xaxis=list(title="Log2 Fold Change",showgrid=TRUE, zeroline=FALSE),yaxis=list(title="-log10(p-val)", showgrid=TRUE, zeroline=FALSE))
+      if(length(significativePoints)>0) phtml=add_markers(phtml,data=dataSignifToPlot, x=~FC, y=~pval, mode="markers" , marker=list( color=log10(abs(dataSignifToPlot$FC)*dataSignifToPlot$pval),colorscale='Rainbow'), text=~description, hoverinfo="text", inherit = FALSE) %>% hide_colorbar()
+      if(logFCthreshold!=0){
+       phtml=add_trace(phtml,x=c(-logFCthreshold,-logFCthreshold), y=c(0,yMaxLimPlot), type="scatter", mode = "lines", line=list(color="coral",dash="dash"), hoverinfo='none', showlegend = FALSE,inherit = FALSE)
+       phtml=add_annotations(phtml,x=-logFCthreshold,y=0,xref = "x",yref = "y",text = paste(c("log2(1/FC=",opt$thresholdFC,")"),collapse=""),xanchor = 'right',showarrow = F,textangle=270,font=list(color="coral"))
+       phtml=add_trace(phtml,x=c(logFCthreshold,logFCthreshold), y=c(0, yMaxLimPlot), type="scatter",  mode = "lines", line=list(color="coral",dash="dash"), hoverinfo='none', showlegend = FALSE,inherit = FALSE)
+       phtml=add_annotations(phtml,x=logFCthreshold,y=0,xref = "x",yref = "y",text = paste(c("log2(FC=",opt$thresholdFC,")"),collapse=""),xanchor = 'right',showarrow = F,textangle=270,font=list(color="coral"))
+      }
+      if(!is.null(pvalThresholdFDR)){
+        phtml=add_trace(phtml,x=c(xMinLimPlot,xMaxLimPlot), y=c(pvalThresholdFDR,pvalThresholdFDR), type="scatter",  mode = "lines", line=list(color="cornflowerblue",dash="dash"), hoverinfo='none', showlegend = FALSE,inherit = FALSE)
+        phtml=add_annotations(phtml,x=xMinLimPlot,y=pvalThresholdFDR+0.1,xref = "x",yref = "y",text = paste(c("FDR pval limit(",opt$fdrThreshold,")"),collapse=""),xanchor = 'left',showarrow = F,font=list(color="cornflowerblue"))
+      }
+    plotVector[[length(plotVector)+1]]=p
+    
+    #save plotly files
+    pp <- ggplotly(phtml)
+    htmlwidgets::saveWidget(as_widget(pp), paste(c(file.path(getwd(), "plotLyDir"),"/",opt$volcano,"_",correspondanceTable[humanReadingContrastsRenamed[iComparison],1],".html"),collapse=""),selfcontained = F)
+    
+    
+    if(iComparison==nbComparisons || length(plotVector)==volcanoPerPage){
+      #plot and close the actual plot
+      if(opt$format=="pdf"){
+        pdf(paste(c("./plotDir/",opt$volcano,"_",correspondanceTable[humanReadingContrastsRenamed[iComparison],1],".pdf"),collapse=""))}else{
+          png(paste(c("./plotDir/",opt$volcano,"_",correspondanceTable[humanReadingContrastsRenamed[iComparison],1],".png"),collapse=""))
+        }
+      multiplot(plotlist=plotVector,cols=1)
+      dev.off()
+      if(iComparison<nbComparisons){
+        #prepare for a new ploting file if necessary
+        plotVector=list()
+        iToPlot=iToPlot+1
+      }
+    }
+  }
+  remove(dataToPlot,dataSignifToPlot)
+  addComment("[INFO]Volcanos drawn",T,opt$log,T,display=FALSE)
+}
+
+rowItemInfo=NULL
+if(!is.null(opt$rowNameType) && !is.null(opt$organismID)){
+##get gene information from BioMart
+#if(!require("biomaRt")){
+#    source("https://bioconductor.org/biocLite.R")
+#    biocLite("biomaRt")
+#}
+
+ensembl_hs_mart <- useMart(biomart="ensembl", dataset=opt$organismID)
+ensembl_df <- getBM(attributes=c(opt$rowNameType,"description"),mart=ensembl_hs_mart)
+rowItemInfo=ensembl_df[which(ensembl_df[,1]!=""),2]
+rowItemInfo=unlist(lapply(rowItemInfo,function(x)substr(unlist(strsplit(x," \\[Source"))[1],1,30)))
+names(rowItemInfo)=ensembl_df[which(ensembl_df[,1]!=""),1]
+}
+  
+#write(unlist(dimnames(data.fit.eb$adj_p.value)),opt$log,append = T)
+
+#prepare additional output containing df informations
+dfMatrix=matrix(0,ncol=3,nrow = nrow(data.fit.eb$coefficients),dimnames = list(rownames(data.fit.eb$coefficients),c("df.residual","df.prior","df.total")))
+dfMatrix[,"df.residual"]=data.fit.eb$df.residual
+dfMatrix[,"df.prior"]=data.fit.eb$df.prior
+dfMatrix[,"df.total"]=data.fit.eb$df.total
+
+#filter out genes with higher p-values for all comparisons
+genesToKeep=names(which(apply(data.fit.eb$adj_p.value,1,function(x)length(which(x<=opt$fdrThreshold))>0)))
+#filter out genes with lower FC for all comparisons
+genesToKeep=intersect(genesToKeep,names(which(apply(data.fit.eb$coefficients,1,function(x)length(which(abs(x)>=logFCthreshold))>0))))
+
+if(length(genesToKeep)>0){
+  data.fit.eb$adj_p.value=matrix(data.fit.eb$adj_p.value[genesToKeep,],ncol=ncol(data.fit.eb$adj_p.value))
+  rownames(data.fit.eb$adj_p.value)=genesToKeep
+  colnames(data.fit.eb$adj_p.value)=colnames(data.fit.eb$p.value)
+  
+  data.fit.eb$p.value=matrix(data.fit.eb$p.value[genesToKeep,],ncol=ncol(data.fit.eb$p.value))
+  rownames(data.fit.eb$p.value)=genesToKeep
+  colnames(data.fit.eb$p.value)=colnames(data.fit.eb$adj_p.value)
+  
+  data.fit.eb$coefficients=matrix(data.fit.eb$coefficients[genesToKeep,],ncol=ncol(data.fit.eb$coefficients))
+  rownames(data.fit.eb$coefficients)=genesToKeep
+  colnames(data.fit.eb$coefficients)=colnames(data.fit.eb$adj_p.value)
+  
+  data.fit.eb$t=matrix(data.fit.eb$t[genesToKeep,],ncol=ncol(data.fit.eb$t))
+  rownames(data.fit.eb$t)=genesToKeep
+  colnames(data.fit.eb$t)=colnames(data.fit.eb$adj_p.value)
+  
+  dfMatrix=dfMatrix[genesToKeep,,drop=FALSE]
+  
+}else{
+  addComment(c("[WARNING]No significative genes considering the given FDR threshold : ",opt$fdrThreshold),T,opt$log,display=FALSE)
+}
+
+addComment("[INFO]Significant genes filtering done",T,opt$log,T,display=FALSE)
+
+
+#plot VennDiagramm for genes below threshold between comparisons
+#t=apply(data.fit.eb$adj_p.value[,1:4],2,function(x)names(which(x<=opt$threshold)))
+#get.venn.partitions(t)
+#vennCounts(data.fit.eb$adj_p.value[,1:4]<=opt$threshold)
+
+#make a simple sort genes based only on the first comparison
+#newOrder=order(data.fit.eb$adj_p.value[,1])
+#data.fit.eb$adj_p.value=data.fit.eb$adj_p.value[newOrder,]
+
+#alternative sorting strategy based on the mean gene rank over all comparisons
+if(length(genesToKeep)>1){
+  currentRank=rep(0,nrow(data.fit.eb$adj_p.value))
+  for(iComparison in 1:ncol(data.fit.eb$adj_p.value)){
+    currentRank=currentRank+rank(data.fit.eb$adj_p.value[,iComparison])
+  }
+  currentRank=currentRank/ncol(data.fit.eb$adj_p.value)
+  newOrder=order(currentRank)
+  
+  data.fit.eb$adj_p.value=matrix(data.fit.eb$adj_p.value[newOrder,],ncol=ncol(data.fit.eb$adj_p.value))
+  rownames(data.fit.eb$adj_p.value)=rownames(data.fit.eb$p.value)[newOrder]
+  colnames(data.fit.eb$adj_p.value)=colnames(data.fit.eb$p.value)
+  
+  data.fit.eb$p.value=matrix(data.fit.eb$p.value[newOrder,],ncol=ncol(data.fit.eb$p.value))
+  rownames(data.fit.eb$p.value)=rownames(data.fit.eb$adj_p.value)
+  colnames(data.fit.eb$p.value)=colnames(data.fit.eb$adj_p.value)
+  
+  data.fit.eb$coefficients=matrix(data.fit.eb$coefficients[newOrder,],ncol=ncol(data.fit.eb$coefficients))
+  rownames(data.fit.eb$coefficients)=rownames(data.fit.eb$adj_p.value)
+  colnames(data.fit.eb$coefficients)=colnames(data.fit.eb$adj_p.value)
+  
+  data.fit.eb$t=matrix(data.fit.eb$t[newOrder,],ncol=ncol(data.fit.eb$t))
+  rownames(data.fit.eb$t)=rownames(data.fit.eb$adj_p.value)
+  colnames(data.fit.eb$t)=colnames(data.fit.eb$adj_p.value)
+  
+  dfMatrix=dfMatrix[newOrder,,drop=FALSE]
+}
+
+
+#formating output matrices depending on genes to keep
+if(length(genesToKeep)==0){
+  outputData=matrix(0,ncol=ncol(data.fit.eb$adj_p.value)*5+2,nrow=3)
+  outputData[1,]=c("X","X",rep(humanReadingContrastsRenamed,each=5))
+  outputData[2,]=c("X","X",rep(c("p-val","FDR.p-val","FC","log2(FC)","t-stat"),ncol(data.fit.eb$adj_p.value)))
+  outputData[,1]=c("LIMMA","Gene","noGene")
+  outputData[,2]=c("Comparison","Info","noInfo")
+  
+  outputDfData=matrix(0,ncol=3+1,nrow=2)
+  outputDfData[1,]=c("X","df.residual","df.prior","df.total")
+  outputDfData[,1]=c("Statistics","noGene")
+}else{
+  if(length(genesToKeep)==1){
+    outputData=matrix(0,ncol=ncol(data.fit.eb$adj_p.value)*5+2,nrow=3)
+    outputData[1,]=c("X","X",rep(humanReadingContrastsRenamed,each=5))
+    outputData[2,]=c("X","X",rep(c("p-val","FDR.p-val","FC","log2(FC)","t-stat"),ncol(data.fit.eb$adj_p.value)))
+    outputData[,1]=c("LIMMA","Gene",genesToKeep)
+    outputData[,2]=c("Comparison","Info","na")
+    if(!is.null(rowItemInfo))outputData[3,2]=rowItemInfo[genesToKeep]
+    outputData[3,seq(3,ncol(outputData),5)]=prettyNum(data.fit.eb$p.value,digits=4)
+    outputData[3,seq(4,ncol(outputData),5)]=prettyNum(data.fit.eb$adj_p.value,digits=4)
+    outputData[3,seq(5,ncol(outputData),5)]=prettyNum(2^data.fit.eb$coefficients,digits=4)
+    outputData[3,seq(6,ncol(outputData),5)]=prettyNum(data.fit.eb$coefficients,digits=4)
+    outputData[3,seq(7,ncol(outputData),5)]=prettyNum(data.fit.eb$t,digits=4)
+    
+    outputDfData=matrix(0,ncol=3+1,nrow=1+nrow(dfMatrix))
+    outputDfData[1,]=c("Statistics","df.residual","df.prior","df.total")
+    outputDfData[2,]=c(rownames(dfMatrix),prettyNum(dfMatrix[,c("df.residual","df.prior","df.total")],digits=4))
+  }else{
+    #format matrix to be correctly read by galaxy (move headers in first column and row)
+    outputData=matrix(0,ncol=ncol(data.fit.eb$adj_p.value)*5+2,nrow=nrow(data.fit.eb$adj_p.value)+2)
+    outputData[1,]=c("X","X",rep(humanReadingContrastsRenamed,each=5))
+    outputData[2,]=c("X","X",rep(c("p-val","FDR.p-val","FC","log2(FC)","t-stat"),ncol(data.fit.eb$adj_p.value)))
+    outputData[,1]=c("LIMMA","Gene",rownames(data.fit.eb$adj_p.value))
+    outputData[,2]=c("Comparison","Info",rep("na",nrow(data.fit.eb$adj_p.value)))
+    if(!is.null(rowItemInfo))outputData[3:nrow(outputData),2]=rowItemInfo[rownames(data.fit.eb$adj_p.value)]
+    outputData[3:nrow(outputData),seq(3,ncol(outputData),5)]=prettyNum(data.fit.eb$p.value,digits=4)
+    outputData[3:nrow(outputData),seq(4,ncol(outputData),5)]=prettyNum(data.fit.eb$adj_p.value,digits=4)
+    outputData[3:nrow(outputData),seq(5,ncol(outputData),5)]=prettyNum(2^data.fit.eb$coefficients,digits=4)
+    outputData[3:nrow(outputData),seq(6,ncol(outputData),5)]=prettyNum(data.fit.eb$coefficients,digits=4)
+    outputData[3:nrow(outputData),seq(7,ncol(outputData),5)]=prettyNum(data.fit.eb$t,digits=4)
+    
+    outputDfData=matrix(0,ncol=3+1,nrow=1+nrow(dfMatrix))
+    outputDfData[1,]=c("Statistics","df.residual","df.prior","df.total")
+    outputDfData[2:(1+nrow(dfMatrix)),]=cbind(rownames(dfMatrix),prettyNum(dfMatrix[,c("df.residual")],digits=4),prettyNum(dfMatrix[,c("df.prior")],digits=4),prettyNum(dfMatrix[,c("df.total")],digits=4))
+  }
+}
+addComment("[INFO]Formated output",T,opt$log,display=FALSE) 
+
+#write output results
+write.table(outputData,file=opt$outputFile,quote=FALSE,sep="\t",col.names = F,row.names = F)
+
+#write df info file
+write.table(outputDfData,file=opt$outputDfFile,quote=FALSE,sep="\t",col.names = F,row.names = F)
+
+end.time <- Sys.time()
+addComment(c("[INFO]Total execution time for R script:",as.numeric(end.time - start.time,units="mins"),"mins"),T,opt$log,display=FALSE)
+
+addComment("[INFO]End of R script",T,opt$log,display=FALSE)
+
+printSessionInfo(opt$log)
+#sessionInfo()
+
+
+