Mercurial > repos > vimalkumarvelayudhan > riboplot
diff ribocount.xml @ 8:844eb8c36f32
Add help section in xml and update usage documentation
author | Vimalkumar Velayudhan <vimal@biotechcoder.com> |
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date | Mon, 17 Aug 2015 10:27:58 +0100 |
parents | 1901dcdc12ff |
children | cff63cf0dea8 |
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--- a/ribocount.xml Thu Aug 13 15:03:20 2015 +0100 +++ b/ribocount.xml Mon Aug 17 10:27:58 2015 +0100 @@ -26,5 +26,47 @@ <data format="html" name="html_file" label="ribocount (HTML report)"/> </outputs> <help> +**RiboCount** + +Output read counts for all transcripts in an alignment. + +---- + +**Parameters** + +1. Ribo-Seq alignment file (Sorted BAM file) + + A Bowtie 1 output (BAM) from an alignment of Ribo-Seq data to the transcriptome. This BAM + file should be sorted. This can be done using one of the following methods. + + 1. RiboGalaxy -> Sort Data -> Sort BAM dataset. + 2. ``samtools sort input.bam inputsorted`` + +2. Transcriptome (FASTA) + + A FASTA format file with sequences of the transcripts. + +3. Read lengths to consider [optional] (Integer - 0 or greater) + + If this option is provided, only Ribo-Seq data of the given length is considered. + +4. Offset [optional] (Integer - 0 or greater) + + If this option is provided, this offset is added to the read alignment positions. + +---- + +**Output** + +Read counts for all transcripts in the alignment (ZIP) + +The output file ``ribocount_output.zip`` should first be uncompressed. This will generate +a folder called ``ribocount_output``. Open ``index.html`` in a web browser to view the results of ribocount. + +Total reads for each transcript will be displayed in a table along with the name of the transcript and a link +to the CSV file containing the read counts in 3 frames for each position in the transcript. + +.. image:: images/ribocount.png + </help> </tool>