Mercurial > repos > vimalkumarvelayudhan > riboseqr_wrapper
diff README.rst @ 5:423ad61697c4
Bugfix 1: [triplet] Lengths (frameCounting) if given should be a range (not zero).
readingFrame function fails with subscript out of bounds.
Bugfix 2: [triplet] Check if transcript name in SAM matches the name in FASTA. Produce
an error message if it's not. This fixes the problem where an empty plot is
produced (no bars).
[ribosome_profile] - A proper error message is now produced if an invalid transcript name is
provided.
Updated test data
author | Vimalkumar Velayudhan <vimalkumarvelayudhan@gmail.com> |
---|---|
date | Tue, 27 Oct 2015 12:21:50 +0000 |
parents | c34c364ce75d |
children | 5a242f289347 |
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--- a/README.rst Tue Jul 21 15:40:22 2015 +0100 +++ b/README.rst Tue Oct 27 12:21:50 2015 +0000 @@ -1,32 +1,40 @@ -riboseqr_wrapper ----------------- +README +------ + `riboSeqR <http://bioconductor.org/packages/3.0/bioc/html/riboSeqR.html>`_ -integration for `Galaxy <http://galaxyproject.org/>`_ and +integration for `Galaxy <http://galaxyproject.org/>`_ / `RiboGalaxy <http://ribogalaxy.ucc.ie/>`_. Included tools .............. -In the order they are run +In the order in which they are run + +#. Prepare riboSeqR Input -1. Prepare riboSeqR Input - Prepare riboSeqR format input files from SAM format alignment files. - The SAM format files should be obtained by aligning Ribo-Seq and RNA-Seq data to the transcriptome. - (RNA-Seq data is optional but required for Differential translation analysis). + Prepare riboSeqR format input files from alignment files (SAM format, + Ribo-Seq or RNA-Seq alignments). + +#. Triplet Periodicity -2. Triplet Periodicity - Plot triplet periodicity for different read lengths. + Plot triplet periodicity for different read lengths. + +#. Metagene Analysis -3. Metagene Analysis + Metagene analysis. -4. Plot Ribosome Profile +#. Plot Ribosome Profile + + Plot Ribosome profile and output Ribo/RNA counts. [OR] - Differential Translation Analysis - Get Ribo and RNA-Seq counts with riboSeqR. Perform differential + Differential Translation Analysis + + Get Ribo and RNA-Seq counts with riboSeqR. Perform differential translation analysis with baySeq. -Dependencies -............ -Tested on Ubuntu Linux 14.04 LTS, 64-bit. Dependencies should install automatically on Linux x86_64. - +Tested with +........... **R** ``3.1.2`` **riboSeqR** ``1.0.5``