Mercurial > repos > vimalkumarvelayudhan > riboseqr_wrapper
diff README.rst @ 0:c34c364ce75d
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author | Vimalkumar Velayudhan <vimal@biotechcoder.com> |
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date | Tue, 21 Jul 2015 14:48:39 +0100 |
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children | d7ce95ccf54f 423ad61697c4 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README.rst Tue Jul 21 14:48:39 2015 +0100 @@ -0,0 +1,36 @@ +riboseqr_wrapper +---------------- +`riboSeqR <http://bioconductor.org/packages/3.0/bioc/html/riboSeqR.html>`_ +integration for `Galaxy <http://galaxyproject.org/>`_ and +`RiboGalaxy <http://ribogalaxy.ucc.ie/>`_. + +Included tools +.............. +In the order they are run + +1. Prepare riboSeqR Input - Prepare riboSeqR format input files from SAM format alignment files. + The SAM format files should be obtained by aligning Ribo-Seq and RNA-Seq data to the transcriptome. + (RNA-Seq data is optional but required for Differential translation analysis). + +2. Triplet Periodicity - Plot triplet periodicity for different read lengths. + +3. Metagene Analysis + +4. Plot Ribosome Profile + + [OR] + + Differential Translation Analysis - Get Ribo and RNA-Seq counts with riboSeqR. Perform differential + translation analysis with baySeq. + +Dependencies +............ +Tested on Ubuntu Linux 14.04 LTS, 64-bit. Dependencies should install automatically on Linux x86_64. + +**R** ``3.1.2`` + +**riboSeqR** ``1.0.5`` + +**baySeq** ``2.0.50`` + +**rpy2** ``2.3.10``