Mercurial > repos > vipints > deseq_hts
diff deseq-hts_1.0/src/deseq-hts.sh @ 9:e27b4f7811c2 draft
Updated DESeq version 1.12
author | vipints <vipin@cbio.mskcc.org> |
---|---|
date | Tue, 08 Oct 2013 08:09:28 -0400 |
parents | 8ab01cc29c4b |
children |
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--- a/deseq-hts_1.0/src/deseq-hts.sh Wed Jun 27 15:38:39 2012 -0400 +++ b/deseq-hts_1.0/src/deseq-hts.sh Tue Oct 08 08:09:28 2013 -0400 @@ -5,7 +5,7 @@ # the Free Software Foundation; either version 3 of the License, or # (at your option) any later version. # -# Copyright (C) 2009-2012 Max Planck Society +# Copyright (C) 2009-2013 Max Planck Society & Memorial Sloan-Kettering Cancer Center # set -e @@ -16,7 +16,7 @@ . ${DIR}/../bin/deseq_config.sh echo -echo ${PROG}: FML http://galaxy.fml.mpg.de Galaxy wrapper for the DESeq version $DESEQ_VERSION. +echo ${PROG}: Oqtans http://galaxy.cbio.mskcc.org Galaxy wrapper for the DESeq version $DESEQ_VERSION. echo echo DESeq performs differential expression testing from RNA-Seq measurements. echo @@ -35,14 +35,12 @@ echo %%%%%%%%%%%%%%%%%%%%%%% echo echo load the genome annotation in GFF3 format and create an annotation object -echo export PYTHONPATH=$PYTHONPATH:${SCIPY_PATH} ${PYTHON_PATH} ${DIR}/../tools/ParseGFF.py ${ANNO_INPUT} ${GENES_FN} -${DIR}/../bin/genes_cell2struct ${GENES_FN} 2>&1 +${DIR}/../bin/genes_cell2struct ${GENES_FN} echo echo genome annotation stored in $GENES_FN -echo echo %%%%%%%%%%%%%%%%%%%% echo % 2. Read counting % echo %%%%%%%%%%%%%%%%%%%% @@ -68,22 +66,18 @@ tmpfile=`mktemp --tmpdir=/tmp` echo "${DIR}/../bin/get_read_counts ${GENES_FN} $tmpfile $@" -${DIR}/../bin/get_read_counts ${GENES_FN} $tmpfile "$@" 2>&1 +${DIR}/../bin/get_read_counts ${GENES_FN} $tmpfile "$@" -echo echo %%%%%%%%%%%%%%%%%%%%%%%%%%% echo % 3. Differential testing % echo %%%%%%%%%%%%%%%%%%%%%%%%%%% echo - echo testing genes for differential expression using given alignments echo "cat ${DIR}/../src/difftest_deseq.R | $R_PATH --slave --args $tmpfile ${DESEQ_RES_FILE} $#" -cat ${DIR}/../src/difftest_deseq.R | $R_PATH --slave --args $tmpfile ${DESEQ_RES_FILE} $# 2> /dev/null +cat ${DIR}/../src/difftest_deseq.R | $R_PATH --slave --args $tmpfile ${DESEQ_RES_FILE} $# rm $tmpfile ${tmpfile}_COUNTS.tab ${tmpfile}_CONDITIONS.tab -echo echo %%%%%%%% echo % Done % echo %%%%%%%% -echo