comparison gff_fmap.xml @ 5:6e589f267c14

Uploaded

5:6e589f267c14

<tool id="fml_gfffmap" name="GFF-map" version="2.0.0">
  <description>features</description>
  <command interpreter="python"> 
		gff_fmap.py $gff_input &gt; $idmapping
    </command>
  <inputs>
    <param format="gff3,gff" name="gff_input" type="data" label="Query file" help="Provide genome annotation file in GFF."/>
  </inputs>
  <outputs>
    <data format="txt" name="idmapping" label="${tool.name} on ${on_string}: parent child id map"/>
  </outputs>
		<tests>
    		<test>
            	<param name="gff_input" value="Feature_ID_mapping_W.gff3" />
            	<output name="idmapping" file="Feature_ID_mapping_W.txt" />
            </test>
    		<test>
            	<param name="gff_input" value="Aly_JGI.gff3" />
            	<output name="idmapping" file="Feature_ID_mapping_R.txt" />
            </test>
        </tests>
  <help>

**What it does** 

GFF-map provides the features (gene, mRNA, UTR's, exon, CDS etc) relationship based on their identifier mapping in a given GFF file.

--------

**Example**

- The features ID mapping in the following data in GFF3::

	##gff-version 3
	17      protein_coding  gene    7255208 7258258 .       +       .       ID=ENSG00000213859;Name=KCTD11
	17      protein_coding  mRNA    7255208 7258258 .       +       .       ID=ENST00000333751;Name=KCTD11-001;Parent=ENSG00000213859
	17      protein_coding  protein     7256262 7256960 .       +       0       ID=ENSP00000328352;Name=ENSP00000328352
	17      protein_coding  five_prime_UTR  7255208 7256261 .       +       .       Parent=ENST00000333751
	17      protein_coding  CDS     7256262 7256960 .       +       0       Name=CDS:KCTD11;Parent=ENST00000333751,ENSP00000328352
	17      protein_coding  three_prime_UTR 7256961 7258258 .       +       .       Parent=ENST00000333751
	17      protein_coding  exon    7255208 7258258 .       +       .       Parent=ENST00000333751

- Will be displayed as::
    
    +-----------------------+---------------------------------+
    | Parent feature type   | Associated child feature type(s)|
    +-----------------------+---------------------------------+
    | protein_coding gene   | protein_coding mRNA             |
    +-----------------------+---------------------------------+
    | protein_coding protein| protein_coding CDS              |
    +-----------------------+---------------------------------+
    | protein_coding mRNA   | protein_coding CDS              |
    |                       | protein_coding exon             |
    |                       | protein_coding five_prime_UTR   |
    |                       | protein_coding three_prime_UTR  |
    +-----------------------+---------------------------------+

--------

**About formats**

**GFF3 format** General Feature Format is a format for describing genes and other features associated with DNA, RNA and Protein sequences. GFF3 lines have nine tab-separated fields::

    1. seqid - Must be a chromosome or scaffold.
    2. source - The program that generated this feature.
    3. type - The name of this type of feature. Some examples of standard feature types are "gene", "CDS", "protein", "mRNA", and "exon". 
    4. start - The starting position of the feature in the sequence. The first base is numbered 1.
    5. stop - The ending position of the feature (inclusive).
    6. score - A score between 0 and 1000. If there is no score value, enter ".".
    7. strand - Valid entries include '+', '-', or '.' (for don't know/care).
    8. phase - If the feature is a coding exon, frame should be a number between 0-2 that represents the reading frame of the first base. If the feature is not a coding exon, the value should be '.'.
    9. attributes - All lines with the same group are linked together into a single item.

--------

**Copyright**

2009-2014 Max Planck Society, University of Tübingen &amp; Memorial Sloan Kettering Cancer Center

Sreedharan VT, Schultheiss SJ, Jean G, Kahles A, Bohnert R, Drewe P, Mudrakarta P, Görnitz N, Zeller G, Rätsch G. Oqtans: the RNA-seq workbench in the cloud for complete and reproducible quantitative transcriptome analysis. Bioinformatics 10.1093/bioinformatics/btt731 (2014)

</help>
</tool>