# HG changeset patch # User wolma # Date 1510442362 18000 # Node ID aa82b2e54055862e26a35e45cb00e28576fc26f8 planemo upload for repository https://github.com/wm75/mimodd_galaxy_wrappers commit b36048cd608ede0ec6f6559648525c9350caae34-dirty diff -r 000000000000 -r aa82b2e54055 bamsort.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/bamsort.xml Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,97 @@ + + takes a SAM/BAM dataset and generates a coordinate/name-sorted copy + + macros.xml + + + + + + mimodd sort '$input.ifile' + -o '$ofile' --iformat $input.iformat --oformat $oformat $by_name + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r aa82b2e54055 cloudmap.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/cloudmap.xml Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,591 @@ + + maps phenotypically selected variants by multi-variant linkage analysis + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + (opt['source']['tabfile']) + + + (opt['source']['plotopts']['plots']) + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r aa82b2e54055 convert.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/convert.xml Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,439 @@ + + converts sequence data into different formats + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + (mode['output']['oformat'] in ("bam", "sam") and not mode['output']['split_on_rgs']) + + + + + + (mode['output']['oformat'] in ('bam', 'sam') and mode['output']['split_on_rgs']) + + + + + + (mode['output']['oformat'] in ('fastq', 'gz') and mode['output']['reads_to_report'] in ('default', 'single')) + + + + + + (mode['output']['oformat'] in ('fastq', 'gz') and mode['output']['reads_to_report'] in ('default', 'paired')) + + + + + + (mode['output']['oformat'] in ('fastq', 'gz') and mode['output']['multisegment_report']) + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r aa82b2e54055 covstats.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/covstats.xml Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,57 @@ + + + calculates coverage statistics for a BCF file as generated by the MiModd Variant Calling tool + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r aa82b2e54055 deletion_predictor.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/deletion_predictor.xml Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,117 @@ + + + predicts deletions in one or more aligned paired-end read samples based on coverage of the reference genome and on insert sizes + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r aa82b2e54055 fileinfo.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/fileinfo.xml Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,102 @@ + + provides summary reports for supported sequence data formats. + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r aa82b2e54055 macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,46 @@ + + 0.1.8 + 0.1.8 + 0.1.8_0 + `__ + +- The `MiModD help forum `__ reachable also via `email `__ + + ]]> + + + MiModD + + + + + + + + + + + + + + +@misc{ +maier_mimodd_2014, +title = {{MiModD} - {Mutation} {Identification} in {Model} {Organism} {Genomes}}, +shorttitle = {{MiModD}}, +url = {https://sourceforge.net/projects/mimodd/}, +author = {Maier, Wolfgang and Moos, Katharina and Seifert, Mark and Baumeister, Ralf}, +year = {2014}, publisher={SourceForge.net} } + + + + + + diff -r 000000000000 -r aa82b2e54055 rebase.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/rebase.xml Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,75 @@ + + from a VCF file + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r aa82b2e54055 reheader.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/reheader.xml Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,270 @@ + + + takes a BAM file and generates a copy with the original header (if any) replaced or modified by that found in a template SAM file + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r aa82b2e54055 sam_header.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/sam_header.xml Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,169 @@ + + + writes run metadata in SAM format for attaching it to sequenced reads data + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + not value or (len(value.split('-')) == 3 and all(part.isdigit() and len(part)==expect_len for part, expect_len in zip(value.split('-'), [4,2,2]))) + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r aa82b2e54055 snp_caller_caller.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/snp_caller_caller.xml Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,191 @@ + + + generates a BCF file of position-specific variant likelihoods and coverage information based on a reference sequence and reads aligned against it + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+ + +
+
+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+ + +
+ + + + + +
+
+ + +
diff -r 000000000000 -r aa82b2e54055 test-data/a.bam Binary file test-data/a.bam has changed diff -r 000000000000 -r aa82b2e54055 test-data/a.bcf Binary file test-data/a.bcf has changed diff -r 000000000000 -r aa82b2e54055 test-data/a.fa --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/a.fa Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,3289 @@ +>chrI +gcctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagc +ctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagcct +aagcctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaa +gcctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagc +ctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagcct +aagcctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaa +gcctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagc +ctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagcct +aagcctaagcctaagcctaagcctaagcctaaaaaattgagataagaaaa +cattttactttttcaaaattgttttcatgctaaattcaaaacgttttttt +tttagtgaagcttctagatatttggcgggtacctctaattttgcctgcct +gccaacctatatgctcctgtgtttaggcctaatactaagcctaagcctaa 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+aaaaaaaagatgaatattctataatatatttagatatattatagagtatt +tatcttattatttatagatatatactttgtatatatctatatta diff -r 000000000000 -r aa82b2e54055 test-data/a.sam --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/a.sam Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,824 @@ +@HD VN:1.5 SO:coordinate +@RG ID:000 SM:N2 +@RG ID:266-1 SM:ot266 DS:mutant strain carrying the vab-3 allele ot266 CN:Columbia University PL:Illumina +@SQ SN:chrI LN:25050 M5:7d9f340160ce45568f9bf48d8e16dcf4 +@SQ SN:chrII LN:25050 M5:ba5a89bf12f55a4bd88ddf14e6d27bd6 +@SQ SN:chrIII LN:25050 M5:ba78739f84b6c850448bec286bdd8798 +@SQ SN:chrIV LN:25050 M5:0807d4df5c3593363a5b015f19ef81c2 +@SQ SN:chrV LN:25050 M5:cf942c1556ef72fd9ba352e4a7d9cf9e +@SQ SN:chrX LN:25050 M5:5ce1b12d74140cfc288b493c16fe280b +@SQ SN:MtDNA LN:13794 M5:199e147d502d88e45047413dc83c039c +@PG ID:SNAP PN:MiModD snap VN:0.1.8.0 CL:paired /var/tmp/batch_dataset_548966_dataset_35_20_0.3 /var/tmp/batch_dataset_54896612531.sam -d 8 -S 200 -n 25 -a 7 -F s -o /var/tmp/batch_dataset_54896615435.tmp -h 250 -c 2 -C++ -M -s 100 10000 -t 2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1314:19049:65217 99 chrI 556 60 100M = 702 246 CCTATATGCTCCTGTGTTTAGGCCTAATACTAAGCCTAAGCCTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAATACTAAGCCTAAGCCTAAG CCCFFFFDHHHHHJJJJJJJIJJJJJJIIIIIJIJJJJJGIIJIIHIJIJJHIJIJJHIJJIIJJJIIIIGGGIJHHHHHFFEFFFEECEEDDCACDDC? PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:56:17087:17617/1 0 chrI 575 60 101M * 0 0 AGGCCTAATACTAAGCCTAAGCCTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAAGACTAAGCC GGFGGGDGFGEFBGFGFGGGGGGFGGEGGGGGGGGGGGGGGGGGEGGFFEFGGFFGEAFGFDEGGGGGGGEEFDDEEEEEGFGGEGEDBEEAEDEECFEBC PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1314:19049:65217 147 chrI 702 60 100M = 556 -246 AGCCTAAGACTAAGCCTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAAGACTAAGCCTAAGAC CCC>AA>;(CCC;C@3HEEEGCIGED=EGGGJHGJIGGCIJIIDFGGIGDIHJIHEGIHJIIEGGIIGJJJIGIGJIJJJIGDIHGIHHFHGFFFFFCB@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:81:6795:13435/1 16 chrI 842 60 101M * 0 0 TTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAGTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATA A:@EEEEEBEEEEEEEEEGEEEEGEEEEEEEED=BFEEEGEEGEGFGGGEEGGGGGGGGFGGGFDDAGG=GGEGGGFFFEFEECBFDGGEGDGDGGGFGEG PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:1:46:9721:15037/1 16 chrI 842 60 101M * 0 0 TTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATA AEEBEBBEEEAAEFEFFFE?FCCCBC?DDBD5DD=D:DDC:CC?C>6EB@CEEEDDEECEEEEE8BFFEEFEEADDCCC@CBCCEEFDFFAEFGFDFGGGD PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:77:15674:6412/1 0 chrI 844 60 101M * 0 0 GAAATGAATATCGTAGCTACAGAAACGGCAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAACATA GFFGGGCGDFGEGDFEEFEGGFEGFEEEACC?BCCDDCDECBECDEFEECEEDEEEEEEEEBEEEEEEAEEEEE=AC:=CBBCCCC@::?CACC@BBBABE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:88:10221:3318/1 16 chrI 845 60 101M * 0 0 AAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCATATATAG AEBABBEEBGEGEBGGGEEEEGEEGGEEGGCEEEFEFEEBGGFDEGAEFGGEDFF=EEGEGGGGGG?DBDFGGFGGDFECCFDDCGEEEE:AG?GFFGDGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:87:4119:14575/1 16 chrI 845 60 101M * 0 0 AAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAA GEEGEDEEGEGGEAEFEB?DEEDBGGBGGFGEFBGFGGBGGGGGFGGEGFGGGGGGGGGGGGFEFAGGGGEGGDGGGGDFGGBFGGGGDFDGGDGGGGGEG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:95:9634:15406/1 16 chrI 845 60 101M * 0 0 AAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAA FEEE?GEFEBE:ECE=BEGGGEGEGBFFFEFAFDFFGEBBGGGFGDGEGGGEEEEE5FFFFCFGAEGGEGGGGGGGFGDGGGFGGGGGGGFGDFFFGGGFD PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:2113:3662:39793 73 chrI 846 60 100M = 846 0 AATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAA CCCFFFFFHHHHHJJJJIIIIJIJJJFGIGIIJJIGIJIJJGEHIHJHEHHHJIIJJJJJIJJIGIJIHHHHHHEFFFFECEEDBDDCCDDDDD?CDDDC PG:Z:SNAP RG:Z:000 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:2113:3662:39793 133 chrI 846 0 * = 846 0 GTGTACTACCGTTTCTGGAGCTACGATTTATTTTTCAAAACACGCAAAAAGTGTTTTTCACTATGTACAAAATCATCTCAAAATCGGAAATATTTTTTGT <<<@@@@@@@@@@???#################################################################################### PG:Z:SNAP RG:Z:000 +EAGLE:2:45:11395:10768/1 0 chrI 846 60 40M3I58M * 0 0 AATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCTAAATAT G?FEGGBGGEFDFDDEDEBEEDFFEC@D=AFFFCFFFFFEAFEDFFEF=FFFFFFFD>DF?FFAFFFFFECCAFE=BEAEEFBDEEFEEEEEEEEBEFDDA PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:47:11205:5807/1 16 chrI 846 60 101M * 0 0 AATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAGTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAG FEEDBBFEAEFDEFEE?EDFEEACFEDAFDDFEFFFFFFFFFFFFEEBFFEFEFFFFFFFEEFFFFEEFFFFFFFEEACAFDGGFGGGGGGGGGGGGDGEG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:69:6499:16127/1 0 chrI 847 60 101M * 0 0 ATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGC GGGGGGGGGEGGGGGGGGGGGFGGGDGGEGGGGGGGGGGGFGGGGGGDFGGDGEGGFGG=EFFFFAG;CGGEBGEEFEFGGGGF?CEBCFFEBFEEEEF?F PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:24:6173:14843/1 0 chrI 847 60 101M * 0 0 ATGAGTATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGC FDGFGGDEGEEG?GGAEEEEBEE?::AB;AADB=CEFFFD=EDFF?EBEEEC@@EFDEEDADEDFE;EAFD::D:BCB?FEGGFEGGGCEEBE:CCEEEDFGG=EFBGGEBFGGGDDG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:29:17996:20445/1 16 chrI 847 60 101M * 0 0 ATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGC EE5?EEBBBB?E=DEDFEFEBADCEECBAAE=E=EBDF?EGEE=GCDFFEDBDFGGDGGGGEF=GGGGGBDGGGDGBGGFGGGGGGGFEGGGEEGEEGGFG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:112:10138:4083/1 16 chrI 847 60 101M * 0 0 ATGAGTATCATAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGC EC?@BEACB:C@=EE?CAECC@CCCE>=A<55CCD?BEE?E?FFA;GGGGGEGEGEEDBGGGGEGBGGEGGBFGDGCCCGEG?GDGDFFGGFFFFFE?EDE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:54:15434:16430/1 16 chrI 847 60 101M * 0 0 ATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGC EEEE@EEEEEEDGEEEGEGDEEGGGGEG?DFEGFGGFFGGGFGGEEFGGGGGGGGGGGGGGGGEGGGGGGGFGGFGBGDGGGEGGGEGGGEGGGFDDGGGF PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:58:7649:14576/1 0 chrI 848 60 101M * 0 0 TGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAGTTGCAATTTTTATAGCTAAGACACTTTTTGTCTGCCCAAATATAAGCA GGGGGGGGGGGGGGGGGGFGGGGGEFFEDFEGGGGGGGGFGGGGGGCFFGGGGEFEGGFGGGGGFGGGGGFFFGGGGEGGGGEEEGGGGGFEGEF?FBBFG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:76:11197:2138/1 0 chrI 848 60 101M * 0 0 TGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCA EEDFFFEFBDADEEEFFBFFFEFFE=C@?CFFFFFFFDF?EEEDEFBDFFFFFFFFFFAEAEDEBC;FFEFEFFDDABFFFFE;CFEFEEBEFBDBDDDEB PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:59:14370:7837/1 0 chrI 849 60 101M * 0 0 GAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAAAACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCATATATAGGCAA FGFGEGGEBGFGFGGDGFFGEED=EA@=DDFFE?FEFFFFFFFFFEEEEEFEEADFAFFFFFFFEEFEEBEEBEFEDEEEFEEEEBEEBE:EBBBB@BBAD PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:79:15239:5534/1 16 chrI 849 60 101M * 0 0 GAATATCGTAGCTACAGAAACGATAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGGCACTTTTTGTCTGCACAAATATAGGCAA BFDGDDFFEDEGGDGFEEFBEFFFFEECECEGAEEGGGGFGGGEEGGGEGGBFGGGGGFGGFGGGFGGDEGGGGEGGFGGGGGEFGEGFGDFGFGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:83:6103:1248/1 16 chrI 849 60 101M * 0 0 GAGTATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAA @>B:9?BBBBDBB?DBEBEEEBDDDDD?FAEEEEEEEEEEEEEEFFEFFFEDFEFEECCDAFFFFFFFFFFEBE?@EDFFEFFEFFFFFFFFF?DFEFFDD PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:60:3721:11022/1 0 chrI 851 60 35M3I63M * 0 0 ATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCA DGBGGGFGFFE=EEEDEFFFEBEEEED=EDFDFDFFFFE:FEFEDFDFFFEFFFFFFEDFFFFEFCAFFBBEEBEFFEEEFEAEEEEEEFEFFEEEEBEEF PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:56:9356:7127/1 0 chrI 851 60 101M * 0 0 GTATCATAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACC GFGGGGGGDGGFGCFDBFFFGDGGDGEFFGGDFEEFGDGGEGGGFGGEEEG?GFGEEEFEED;@EGEBEEEEE?BDDDECEEEEEGEE?EA?AC@CCA5?? PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:26:13486:6669/1 16 chrI 851 60 101M * 0 0 ATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACC GE:EEEDDDDAEGEBEEEFEGEEDABFCDEEEEEGGGEGGEEGGFGEDEEFAGDGEGBGFFGGGEGEGGGDFACCFFFGGDGGGEEBGGGEEEEEEEE?EE PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:90:7286:17726/1 0 chrI 852 60 101M * 0 0 TATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCA GGGGFGGGGGGGGGFEGFFGFDDBGGFGGGEFGGGDAGEGGGFDGGDGGGGGGGE?GGGGGGEGGGGDFGGGGEEFBCEEDGFFBGGDGDGGEDBEEGDEB PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:60:11520:19697/1 0 chrI 852 60 101M * 0 0 TATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCA DEDEEFFFFFEGGGFFFFFFBEE?EEEEEEFBFFFFFFF?FFFFFCFFCFFDFFAEFFFFEEADFDDF?EFFFEFFFBEEEEFEFFEFF@FDED:BEEBEE PG:Z:SNAP RG:Z:266-1 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1102:21095:33794 73 chrI 853 60 100M = 853 0 ATCGTAGCTACAGAAACGGTAATACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGGCACTTTTTGTCTGCCCAAATATAGGCAACCA @@@FDFFFHHFHHJJJIJJIJJJJJJJIIJGGIIGGIIII4?GIJJIIJIJJJJGIJJGGIIJJJJJDEHGEFCDCEDEEEEDCDDBDDDFEDCDDDDDB PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1102:21095:33794 133 chrI 853 0 * = 853 0 GCTTCACAGTATATTTTACTTTCTGTGGCTTCACAGTATATTTTACTCTCTGTGGCTTCACAGTATATTTTACTCTCTGTGGCTTCACAGTATATTTTAC A>;6;6(CA;;@B?3DB=AA7EGGIJIIGGIGEIJJJJJHEGGCBFGGFBBFG@IGDJIIJJIIJJIJJIGHIGHGJGHGIHCJJJIHHHHHFFFFFCCB PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1304:18505:45478 73 chrI 853 60 33M3I64M = 853 0 ATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAA @CCFFFFFHHHHHIIIIJJHDHCHIIHIIJIJGIEGGHEDFFGEGIIJIJJJJGIJGGGIJIGEGGGJIHDHEH;?CDFFDECECAA?>BBDDDE>:CCC PG:Z:SNAP RG:Z:000 NM:i:6 +BS-DSFCONTROL03:121:D0M8KACXX:5:1304:18505:45478 133 chrI 853 0 * = 853 0 GGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTC ?;;5::>CDED@DEFDDDDFFFFFFHHHHHJIIIEJJIJJJJJJJJIIHFIGGIIGJJIGJIJJJJJIJJJIJJIJJJJIJJIIIIIHFFAHFFFFFCCB PG:Z:SNAP RG:Z:000 +EAGLE:2:96:9394:5489/1 16 chrI 854 60 101M * 0 0 TCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAA EGEBEEEEEFEGEBEGEEE:E?DFCFFFGEGGFGGGFGEFGGGFGEEEGGGGEGGGGFGGGGGDGGGGEEGGGGGFGGGGGGFGGGGGGDGGGGG?GGGGG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:110:3453:13829/1 16 chrI 855 60 101M * 0 0 CGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAA EAEAE?5EEEEC5=EBDFFDFDGEGEGGDBEFGGBFAEEGGGGGGEEEEDEE8FFFGGGGFGEGGGFFEEEDG?GGGDGGGGGGGFGAGDEGGGAFGEGGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:34:16346:19478/1 16 chrI 856 60 101M * 0 0 GTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAA DDD?BB@B=BBB>DDC@@B?:A?:AAEEDEABCCB6EEEEEBEEDDED:DC>EEEEBDDCBD5CEBED=CEEBEEEEEEDEAEEAEEFGGFBEBEAEEDEE PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:75:1484:17855/1 0 chrI 857 60 101M * 0 0 TAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAGTTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAAT DD@CADEEEEEEFFDBDDCDEEDEEAFDEFAFFFFDFFFFFFD=FDFFFFFFFEF;CCEEDDDFDBEAAFFFFF8EBDEDDDFFAFDFFBFFFEF5FFEF= PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:110:6822:2912/1 16 chrI 857 60 43M1I44M1I2M1D10M * 0 0 TAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAAATTGCAATTTTGATAGCTAGGACACTTTTTGTCTGCACAAATATGAGCAACCAAAAA DBFBDCECFEDEFGFGEGFEGEGGGGEFFGGEGF=BGGGGGGGGGFGGGGGGFGFGGGGGGGGGGEGCGGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:12858:84933 83 chrI 858 60 100M = 876 118 AGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAGTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAAT CCCEEDDDDBDBCDEDDEDCDDDDDDDCEEEEEEEDFHHHHHJIJIJJIHJJJJJJJJJJJJIGGBJJJIJJJJJJIIJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:4 +EAGLE:2:91:13868:11992/1 0 chrI 859 60 41M1I59M * 0 0 GCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAAATTGCAATTTTCATAGCTAGGACACTTTTTGTCTGCACAAATATAGGCAACCAAAAATA GGGEGGGBEDGGECEEDFFFGGGGGGGGFGGGEGGGGFFGGGGAG=FFBDFFGGGEAFGBGEGBGGAEGEGFEEEEDEEEDFF=ECBCCC?AECCCDDECE PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:119:12921:6612/1 16 chrI 859 60 27M3I71M * 0 0 GCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAA BBEEGEE=FBEEFDFGFAGBGGGFEGEFGDGEEGGAEGGGDGGGGDDFGDGGEEGEGGGEGEEEEBEBCCEFDGFFGDGGEFFGGFEEEEEFFBFFGGFGF PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:111:6913:8666/1 0 chrI 860 60 101M * 0 0 CTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAAT GGGGGGFFFGFF@EEAEEEEBGGGFGGEGGGGEGGGDGEDCFA5F?EEEEGGG>GGBFEEFFEGGEFFAFF>EDEC?FEDABBBBD=A:D=B=EB@E@?;> PG:Z:SNAP RG:Z:266-1 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:2216:18829:62698 83 chrI 860 60 100M = 871 111 CTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAA CEDDDC?BBBCDEEEECC@EDEEECEFFFFEFHHHJJJJJIJIJIJJJJJJJJJIJJJJJIGGGJJJJJJJJJJJJJIIJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:3 +EAGLE:1:67:7435:14824/1 16 chrI 861 60 101M * 0 0 TACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCATATATAGGCAACCAAAAATAATT ::GEEGEEGDGGGEGEGDDBGG?GGGGFGGG@FFAFEGGFFEFGDGEFGGGFFGGGFFFFFGBFGGFGDGGGGGGGGGEFGGBGGGGFFGGGFGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:2 +EAGLE:2:11:15002:3991/1 16 chrI 862 60 101M * 0 0 ACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTT DEEFEDBEFEFDFBDF?EEFFDG?GEFEFC=EGAFGEGDFDFGGEEG=?GGGDDDFCEEBE6@@>A=GFFGGCFGGEEEEE:E?EEAEDEECEEE?5EEEE PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:96:4105:9079/1 0 chrI 863 60 101M * 0 0 CAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTC GGGGDGGGGEGGEGGDGGGGGGGDGGGGGFGGGEGGFEBGDGGEGEG?GEFEEGEEEEEGDDEDEFEBEECC=DFDDDCCEAEBGEECGE@DEF???;?BE PG:Z:SNAP RG:Z:266-1 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:1204:8976:92778 73 chrI 864 60 100M = 864 0 AGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTC BCCFFFFFHHGHHJJJJJJJJJJJJJHHGHHJJJJJJJJJJIIJJJJJJJIIJJJIJJJJGIJJJJJHHHHHHFFFFFEEEEDEDDDDDDDBBDDDEDEC PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:1204:8976:92778 133 chrI 864 0 * = 864 0 AGAGTGTACTACCGTTTCTGTAGCTATGATACTCATTTCAAAAAACCCGAAAAATGATTTTTTCAATATGCAACAAATCATCAAAAACTTGGAAATTATT BCCFDFDFHHHHHJHJIIJJJJJJJJJJJFJJJJJJJJJJJJJIJJJJJJJJJJJIJJJJIJHFFFFFFECDCEEBDDDDDDDDDDDDDDDDDDDDDDED PG:Z:SNAP RG:Z:000 +EAGLE:2:89:4630:12148/1 0 chrI 865 60 101M * 0 0 GAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCA GFEGGFFFFFB=DDCGGGGGEEFFFDFFFFGGDGGDGG=EDCGGGGGEG>GGFGFBFFFBGEGGGDCC=FEEEEEEE=E:EBEEEFGF?GGCAA8;EEBE: PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:71:6779:3992/1 0 chrI 866 60 20M3I78M * 0 0 AAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTC GGGGEFGGFFEGGFEGGGGGBGFEGFGGFEGGFGEACCFFDGEBFGGGGFGEGGADBFEFGGEE5EEE=ABBFEGGGGBFEE=EEEDGGBEEE=E@B;CAE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:90:8794:13563/1 0 chrI 867 60 101M * 0 0 AACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAG FFFFFEDFFFGFFFGGGGFFEGGGFFGGGGFGDGEFFGGAGGDGFGGGGFFCGGDGGGBGDF:F;ACEEEFF:AEDEEAEEDECGEGFGGE8C;DD=BD?B PG:Z:SNAP RG:Z:266-1 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2310:12542:49797 73 chrI 869 60 100M = 869 0 CAGTAGTACACTCTTCTCAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGT BCCDFFFFHHGHFJJGGJJJJJHJIJIJJJIIJIJGIJJJJJJJJJIIIJJJIGHHGGGIGEHGIGEHEGHFGGHCCFBDFFEDDDDDDDDEFEDEDCCD PG:Z:SNAP RG:Z:000 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:2310:12542:49797 133 chrI 869 0 * = 869 0 GTGTCCTAGCTATAAAAATTGCAAATTTTTTGTATTTTCAGAAGAGTGTACTACCGTTTCTGTAGCTACGATATTCATTTCAAAAAACCCGAAAAATGTT CB@DFFFFHHGHHJJJJIGGIJJIJIGIIJJJHIIIJIIJJHHIIIGHHIIJJJJJJJJJJJIJJJJJJJIHHGHFFFFFFFEEEDDDDDDDDDDDDDBD PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2216:18829:62698 163 chrI 871 60 100M = 860 -111 GTAGTACACTCTTCTCAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTT CBCFDFFFHHHHHJJJJJJIJJIIJJJJJJJJJJJJJJJJJJJJJJIJJIDHGHIIJJJJJJJIJJJEIIEEHHHHGFFFFDDDDDDDDDE<@DC5@@>> PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2306:15570:52084 73 chrI 871 60 100M = 871 0 GTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTT @@CFDDFFHHHHHJJJJJJJIJIJJIIJIJJJJJJJJJJJJJJJJJJJJJIIJGIJJJJHHFHHHFFFFEEEFEDCCCDBDDDDDDDDDEEEDEECDDDC PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2306:15570:52084 133 chrI 871 0 * = 871 0 TTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTT BBDDDDDDDBBCECEEEEFFFFFFHHHJJJJJJJJJJJJIIIJJJIIIGJJJJJGJJJJJJIHFJJJIGJJJJJJJJJJJIJJJJJJHHHFHFDDFFCCB PG:Z:SNAP RG:Z:000 +EAGLE:2:85:11800:16523/1 16 chrI 871 60 101M * 0 0 GTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTT GEGGEEFEGEGEEGEGGFGGE@GGGGGGGEGGGFGEGEGEGGGGGGGFGGEGEGEGGFGGGGFGGGAGGGGGGGGGGGGGGGGGGGGGGFGGGEGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:50:15754:14085/1 0 chrI 872 60 101M * 0 0 TAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTT FFFFFFFFDFFAFFFFBDFFFFFFFFFFFFFFFFBFFFFFFFEFFFFFFFFFFEFFFFFEEFFFFFFFFFEFFBFEFFEFFF?FFDEEEEFFEEEF@DFEA PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:51:4256:7814/1 0 chrI 873 60 101M * 0 0 AGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATTATTTCCAAGTTTTTG EFFDGGGGGEGGGFAFFGGGGGGFFGGGGFGGF?DGFGGGGGFGGFFAFGDGEDEGGGEGEFGGGEGGGDDFGGFGGEGBGGEFBGFEEEEEEBEEEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:83:2757:15592/1 0 chrI 874 60 101M * 0 0 GTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGA FEFFFFDFFEGBGGGGFDGGGEFFEDGGDGFBEAFFFFEACDGDEEDGGDEGEGGGGEEDEEEDDAE?EDBEEEBEEEBBGEEE@@@@BDD@?B@@BBB@; PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:74:11870:7997/1 0 chrI 874 60 101M * 0 0 GTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGA GGEGGGGGGGGGDFGGGGFGGGGGGFDFFGGGGDFGGGFGGGGFGGGFGGGFEGGDB@GEGEGGGBGEFGEEGEEGEGEEBFEDCEEEE?EC@FACFDFDC PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:12858:84933 163 chrI 876 60 100M = 858 -118 ACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGAT CCCFFFFFHHHHHJIHJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJHHHHHDFFFDDEEEEEEDEEDDDDEDDDBC PG:Z:SNAP RG:Z:000 NM:i:4 +EAGLE:1:89:11320:10564/1 16 chrI 876 60 101M * 0 0 ACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATG @EEFEGFEGFEG=GDFEGGEGGEGEGFBGGGEGFGFGGGGGGGGFDFEGGGGGDGAFGGFFGFGGGGFGGGDGGGBFGGGGGFGGGGGGGGGGGDGDGGFG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:87:16027:10849/1 16 chrI 880 60 101M * 0 0 TCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAATTTTTGATGATTT ?BBBB@BBBB?;@A:C?BC??DBEABDE=EEDDDD??DDDBD PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:98:7014:9919/1 16 chrI 880 60 101M * 0 0 TCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTGAGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTT =BGECGFEEGG>?EFEAFEFAEGGABG@DBEFDGDGGFEEGEDCCGGFGDGDEEEDEFGFGDGGGDGGGFGGEGGGGFGGFDGGFGGGGGGEDGGGGGGGF PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:53:7732:11243/1 16 chrI 882 60 101M * 0 0 TTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGT E@D=DF@BB:8EFDEEBB==B?FEEEAABBAEFFFDECEEEBB@EDCE?CAE:EAE?AE=EEEE?ED=D<>EDAF?DDFBFAE?EE??BFAEEDEEFEDF? PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:65:9151:20950/1 0 chrI 884 60 101M * 0 0 CTGAAAATACAAAAAATTTGCAATTTTTATAGCTGAGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTG GFGGGFGGFGGGEGEGGGGGGFEGGGEGGDGGFGG?GGGGGGGGGFGEGGGGGGGGGFGFGFGGFGFGGEFGGGEE@EDFEBBE?EEEEEBBEBDEEEEE? PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:96:16607:9367/1 16 chrI 884 60 101M * 0 0 CTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTG GEEGEGFCCFAGD:GEEAGFBGGGFGE=DDE:BFFFDCE5@AAFBDAEFBCCGFFFAFFFEFDDFDDBB@ABF?FDEGAGEFFFFAGGGDGEFAF?DEFFF PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:99:9928:13510/1 16 chrI 884 60 101M * 0 0 CTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTG GEFEEGEEEGG?GGGGEBEGEFEEEBEGFGGEGFGFFCFCEEFFFFGFGBGFGEGFGGGGFGGEGGGGDGGFEGGGGGBGGGGGGGGGGGGEGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:88:16505:15331/1 16 chrI 885 60 101M * 0 0 TGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGC BEFGEDEEGGEEDEEDDEEEDEEGEEGBGGEEEEEEDF?CCGGGGGGFGGFFGDDGGGGGFGGGGGGFFFGGEFDGDEFFDGFFGGGGGGGGGFFGGGDGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:78:3200:13969/1 16 chrI 886 60 101M * 0 0 GAAAATACAAAAAGTTCGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCA @B:BA88EEEE?=BBB=CEB8>DE?EEFCBFCBCCBE@B@C;DEFFFEFFFBFFDFFFFFFFFFFEEEBFFDEE5EBEEEEEAEE?EEE?DD;DEEFEF=D PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:87:17306:17581/1 16 chrI 886 60 101M * 0 0 GAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCA AABE=8;AAA==A=AAAAAEEEEEBD?BB: PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:54:4351:16632/1 16 chrI 887 60 101M * 0 0 AAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCATATATAGGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCAT EGEGAEGGGGGEGGEGGGGEGGGGGGGGFFGGGGGGEGGFFGGGDGFGFGGGFGFGGGGGGGGGGGDDFFF:EGGEGGGEGFFGFGGGGGGGGGGGGGDGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:115:17415:17791/1 0 chrI 888 60 101M * 0 0 AAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATA DD@D7EEEEE>CCEFFFFDFFFFFF8EFFFEFFFFFFBFFBFF:DDFFFFFFDFDFF?E@DEBAEFDFF;C@C?EE=DADEEEE;D:DD@EEE?BE>E=ED PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:9:16465:5809/1 16 chrI 888 60 101M * 0 0 AAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATA D?FDGDGGGGFDGGGBB=DGEFEGGGFFDFFEEEECD=GEGGFGGDGGGGG?FGFGGFGGG?GGGGGGGEGGEGFGGGGGFGGDBGFEGGGFFF:FGGGBG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:98:3753:20118/1 16 chrI 889 60 101M * 0 0 AATACAAAGAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCATATATAGGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATAT BGBDFGFFFBF=BDFGGEFFGGG?GE?FDEFECEBB>FF=FEDGFDF=EFBGFGGGEEDADE>AAEFDGAGDBGFGDFFFE?FDEEGEEDDFEDFFDADG= PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:33:4809:11289/1 16 chrI 890 60 101M * 0 0 ATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATT =EEFEGFFF=BDBBGG=EGGBEDGGG:DBEF?GCFGGDGFGEGEFGGEGGEGGDGGGDFDGCGGGGFFGGGGGGGGGGDFGFFGFGGFGGGGDGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:71:4682:12647/1 0 chrI 891 60 101M * 0 0 TACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTG GGGGGEGGGGGDGGGGGGGDGGGGGGGGGGDGGGFGGGEGAGGFGGGFGGFGGFGGGGGGGGFGGGGGGGGGDGFAEA:CEEECEE9CCECABCBDDDCDD PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:75:5406:10078/1 0 chrI 891 60 101M * 0 0 TACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTG GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDEGGGGGGGGGGGGEGFGGDGGGFGGGGGGGGFGGGBGCGFGGEEGGFEGGEEGCFFGFFE? PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:39:9088:2610/1 0 chrI 891 60 101M * 0 0 TACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTG ECE??D>A<>EEE:EDEEEEDEBBCE=EEEEE?DEEEEED@B=EADDEEEA;EEEDEEEE=EDD=EECCADDED?BAACDDB6?@?5DDBBEAE=EE=EAA PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:66:8142:4981/1 16 chrI 891 60 81M1D20M * 0 0 TACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAGGCAACCAAAAATAATTTCCAAGTTTTAATGATTTGTTGCATATTGA >@DFGGE=EA:FEDFCABBE?DBEE?EEEEEFBCCFADFEGDFF?CEEEEBE?EEEDCAA>GFDEGEADEDFDFDAFBADFGFAEFFFEDEDDDEEFDFFE PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:77:9937:16848/1 16 chrI 895 60 101M * 0 0 AAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAA BEEEEEEEEEEEEEEEEEDEGEEDFEAEB5>5DCCFE:AFGG:GGGG?FGFDGGGEGGGGGGGGGGGGFFGGFGGEGGGGDDGGFGGGGGGGGGGGGGGGGGGFF PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:108:8721:8141/1 16 chrI 898 60 101M * 0 0 AATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAA D5:B=AFC?CF?FFFFE=FFFAFFDDDF=DFFFDFEFF=BFFFCFFFFFFFFFCFAFFFDFFFFFFABAFFADAEDFFFDFED?FFFFBBEDFEEDGFEGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:41:18519:16779/1 16 chrI 900 60 101M * 0 0 TTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACA BEEFFFE=BEEEBEEFEEAEEBD=>>EFBAF:FBFF:FFFFDDFDFFFFEEFFFFFFFEFFFEFEFFFFFEFFFAFFFFDFFFFFEDFFFEAEEEBEDEDE PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1112:7827:74939 165 chrI 901 0 * = 901 0 CATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTGAGACACTT CCCFFFFFHHHDFIJJJJJJIIJJJJIJJJIIIJJJIJIIJJJJJCGIAGFHIIJJJJIHHHHHHFFFFFDCDDDDDDEDDDCDDDDECCD@3?@:<=??CCCAFDEFF=F?FFDF=D?AE?E@CFFABFEFFFEEE=C5@CA@FFFFFFFEE??BEDADA=DBEE?EBBEBEEDF>DBEEDEEEED:DEC@@5E PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:41:11602:1908/1 16 chrI 904 60 101M * 0 0 CAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTT EEEEEGDDEDBFEDBEDAE@CEEGEEEEGEFGGGGBGGFGGGGDGEGGGGGGGGFFGGFGGFGGGGGFFGGGGGGGGGGGGFGGGGGGFGGFFGGFGGFGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:51:19603:11640/1 16 chrI 904 60 101M * 0 0 CAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAAAAATTT EEEEEED=DBBAEBBFE?E8DDEDDF PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:96:7398:5258/1 0 chrI 907 60 101M * 0 0 TTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCG EGGGFGGFGGGGGFGGGGGGGGGGEGGGGGGGGDGFGGGGGGGGGDEGDFGG=GGGGGFEGDGGGGGEFGEGGGGAGDFFGFEECGEDEGGEEEEFGFDDG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:59:12995:3551/1 16 chrI 909 60 101M * 0 0 TTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGG GFGFEFDFEEEEDDCCCEEE?GDFEFDDBGEEGBGGGGGGGFGGGGFEGGEGGGGGGGGGGGGFGGGGGGGGGEGGFGGEGGGGGGGGFGFEGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:80:3409:18738/1 16 chrI 912 60 101M * 0 0 ATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTT EEEEFEDEBAFA@@DEEEEEGEFFGEGGFEGGGGGGEEDGGGGGFGEGGGEGFFGEGFGGGGGGGFGGFGGGGGBGGGGGGGGGGFEFFFEDFGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:77:1620:5914/1 16 chrI 913 60 101M * 0 0 TAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTT ??@@??FFEFE?EDE@>ACCAEFFFEEEEE=FFFFE:EEDDFEEFEEEEDDDDDB?:>>?ACC>C?DE?= PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:103:12336:20768/1 0 chrI 917 60 101M * 0 0 TAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGA FGGGGGGDFGGGGGGGDGFGGGGGGGGGGGGGGGFFGGGGGGCE=DGGDGGEGGDFA?EFGGBEDEGGGFEG?@GEE?FFBGGGDGFBGGBFF?A:A>;@E PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:33:12668:17941/1 0 chrI 918 60 101M * 0 0 AGGACACTTTTTGTCTGCCCATATATAGGCAACCAAAAATTATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAA FGGGGGGGGGGGGEGEGFEGDGFFDDBA?FFGDFGGGECDGFDFFFGE=G@EEDEE=BEE=EEECE?A:F=DEDD:E?EEC;@;DDEEADBE5@BEEB:5F PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:70:1514:14146/1 0 chrI 919 60 101M * 0 0 AGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAA DFAAFFDFFEEEC>E=DDDDDEEEDDEDEE?DEEEE?EEE@6CBBEABEB=AAAADBEEBEEDEDEE:EED@BE=AB?B8C?=EEEEEEEA<:@B?????: PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:41:3640:5327/1 16 chrI 919 60 101M * 0 0 AGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAA 5FFF?CFFFFDEEDCE5=DE=EEEEDEEEEB?>@@DAEEDDBFAFFDDDD?DEFFDFFF>FEEDBEDEBFFDDDDD=C:A?FFDEFDEEDBEDEEDBBECE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:70:16168:12341/1 0 chrI 920 60 101M * 0 0 GACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAAT GGGGGGGGGGGGFGGGGGGEGGGGGGEGBGGGGGGGEFGFGGGGGFGGEFGGGF=FFDBFFGCEGDEGFEEDBEGEBEE=@EGGEEBEFE==BBEDB:DAD PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:6:3780:20120/1 16 chrI 920 60 101M * 0 0 GACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAAT C=BBD@EGEGEFDFC=DDDEBD5?DBE PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:98:18353:7963/1 0 chrI 922 60 101M * 0 0 CACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAACAATCATTTTTCGGGTTTTTTGAAATGA @@C@>ACC:9>D:BDC:DD:DA?ABD?DDDD?BDDCC@>A?DDC5A<5A?BB?BBA=ACA@BBBBCC???@@??@A?AABBBBB@:=?8ABBBBB@@BB?? PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:84:5126:1325/1 0 chrI 922 60 76M1D25M * 0 0 CACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAACAACTTTTTTCAGGTTTTTTGAAATGAA GGGGGGGGGGGGFGGGGGGGGGGGGFGGFGGGGGGGGGGGGGGGFGEEGGGGEEGFEGFGEGGFEEGEGG@BAEEEEEEEEEFEECD@9CCDDD=EEEE?? PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:12:11478:10672/1 0 chrI 922 60 76M1D25M * 0 0 CACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAACAACTTTTTTCAGGTTTTTTGAAATGAA CAC?=:BBD;EFDFEFFDDFADFFEFFFFEFEFFDFCC=EFFD?EEBA?DDFCA;AEEB:DBFDEFEEBADBBDEEDF5=BEE?E?B=@B@BCC@B=ACB? PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:95:5316:12215/1 0 chrI 922 60 101M * 0 0 CACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGA GGGGGGGGGEGGGGGGGGGGGGGGGGGGGGGFBGDGGGFGDGGGGGEGGGGGEGGFEGGGEGGGEGFEGEEFBEEEC=;CEEGEEEDFA@CCCE=C=AEED PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:113:5213:14267/1 16 chrI 923 60 75M1D26M * 0 0 ACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATATTTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAAT =:;AABB??EB?B=DDA:EEEDDDEBB?EEEDEGGGEFEEGFEDC@@EFEFGGEEGCDB?ABA?5ADDGGGEF?AFGFGGFDFABFBDDGGF?GGGFGG5F PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:118:8721:7001/1 0 chrI 924 60 101M * 0 0 CTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAAT GGFDGGGCGGFGGFGGGDGGGGDGGGGFGBGGGGGADFFGGGGGEGFDFGEEGBACECBEBADFFEEBGEBBDBAC@CGGDBBDGB>EEEDFDDF@E:D@8 PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:79:1079:9916/1 16 chrI 924 60 74M1D27M * 0 0 CTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAACAATTTTTTTCAGGTTTTTTGAAATGAATA ;@EAEEEEGEEEEBEGGEGEGGAFEE@:@:;FEEFDEDEDGEGGFGBEBGGBGFGGGEDBGGEFGGGGGDGGGEFGEGGGGGGFGGGGDFFGGGFFGGGFG PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:85:1256:19207/1 16 chrI 924 60 74M1D27M * 0 0 CTTTTTGTCTGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAACAACTTTTTTCGGGTTTTTTAAAATGAATA ECEEC=FEFGDEGFEDCGFDFGFBGEGEEEGEFBGEEGDGFEEGGGGGBGGAGGGGGGBDGFGGGGGGGEFGGDGGGGGGGFGGGGGGGGGGFGGFGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:48:19056:2032/1 16 chrI 924 60 101M * 0 0 CTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAAT C;BAAAB:EDDEGEEEDAE@EDGGGBG:GDEGGEFEEFAGEAGGBGGFCEEGFDGGFFGFGGFGEFEECCAEDE@EGBFDGGDGGGGGEAEGGGFEGGGGF PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:76:11456:6131/1 16 chrI 924 60 74M1D27M * 0 0 CTTTTTGAATGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATA EEEEEEGGEEGDGGEEDGGEFFGGEEGEGEGGGEEGEGGGGGGEGEFGGGGFGGGFGGGGGG?GGGDGGGGFFDFFGGGGGGGGGEGGGGGGGGGGGFGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:61:3268:15848/1 16 chrI 924 60 74M1D27M * 0 0 CTTTCTGAATGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATA FEEDFAGDGEFEEEEAEGDEGGEGGGGGFGGEB=EACEEEBDGGGGEGGFFEEEE?DGBBFGGGDGGGGGGDDCD;DFFF?GGGGGDGGEGDGGGFDFFGG PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:1:86:4115:1680/1 16 chrI 925 60 101M * 0 0 TTTATGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTA ?AA9BACFCCFGEEGFCFDFEEGDEGEGGGGDDGEEEDGGGFGGGFGDGGGGGGGFGGFGFGGGGFGGGGGGGGFGGFFGFGGGGFGGGFGGGGFGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:2:10237:11117/1 16 chrI 925 60 101M * 0 0 TTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTTAAATGAATA @:FFFFFDE?FFAFFFFFDDDDFEFFDADEEBFEFDBD?FFFFE?FFFDFFDDFBFEFFFFEAADECDGGGGBEFAGEFFE?GGEFGEGGEGFGFGGFFFFBFGDGGGGGGGE=EDEEEGAGGGFGGDFBBFGGDGGGGGFF PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:11:6740:20191/1 16 chrI 927 60 101M * 0 0 TTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATC AA?@?BAAB=A?::::??B?AB>E>??@EAA@:@?<@>>==@@:CA=D?DBDBB@EEGGEBGEEEE?AEBAADDBBGDBGGGEFGGBGEGGEEEEE5DABD PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:39:17435:10955/1 16 chrI 927 60 101M * 0 0 TTTGTCTGCCCAAATATAAGCAACCAAAAATATTTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGATTTTTTGAAATGAATATC E=D@DFEE=:?GDEBFEEEE?E=ECCBEGDBGGDFBGGGGEFGDGFEEBEAFGEGGGGGGGDGFGAGGGFFEGGGDFGGDGEEEE:GFBGGGDEGFGEGGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:83:18991:16122/1 16 chrI 927 60 74M1I26M * 0 0 TTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAAAAATTTTTCGGGTTTTTTGAAATGAATAT EABA5?DEFFEEC5@BDDFEFDFECCFFFFFACA:?=EFFFEBDEAFBFBBEE=DEAAF=FBDDA>F>BFFEEEED?DD5?GGGGF=EBGG?BGFFFEFFB PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:23:6768:7044/1 16 chrI 929 60 101M * 0 0 TGCCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTATCAT FBB??E?CB@??C=B=C?=?:E=CCD@AA?:5E5EDF=FFFDFFFBBEBEEEC:EEEBCBAFFEFDDFDDFADEEFFBFDFFEFFDFBBEFGDGGDAFFDF PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:47:19241:12210/1 16 chrI 929 60 101M * 0 0 TGAATGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCAT GGGEGGEEGFEEGGGFGGGEEFGGEGGGGGFGGGGGFGGFGGGGGGGGGGDFGFGGGGGGGFGGGGGGFEFGGGGGGGGGGFGDEEGGGGGGGGGGGFGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:79:9772:3464/1 16 chrI 931 60 101M * 0 0 TCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAG =ECCBB=?FGFG?GGGGGBGGFEGDCBFFF=EFBFGGFGGGDFGFFGGGGGEFGGGGGBDGCCCEEFEFFEFFEFDGGGGEEGGDGGGGEGDFGGE?GFGG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:29:4194:2200/1 16 chrI 932 60 101M * 0 0 CTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGC @:AE5DDEEBE>@@=5BBB8G@DFDFGGEEBEE=BB=GGGEEEEDEFEFFFADGBGEGGGGFBFGAGFGGGGGGDGDEDFGGGGGGGGGGGGGGEFG?GGF PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:32:7810:14929/1 0 chrI 933 60 101M * 0 0 TGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCT GGGGGGGGGGGGGFFGEGGGGGGGGGDFGGGGGGGFGGFGDFFDEGEGGEGGGGGGGFGGGBEGGGECGFGGGFGGGB5GFFFFA:CEE:B5?B?FDEAFE PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:38:7229:15314/1 16 chrI 933 60 1I65M1D35M * 0 0 ATGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCT G?EFEDFE=FFFDGFGGBFGGCFDBAGG=FEEGDGFGGGGGFGGGG?G?GBEFEAEGGGGGEEEAAGGGDGGGGGGEGGEDGGGGGFGGFGGGGEGGGEGE PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:45:5717:2359/1 0 chrI 935 60 101M * 0 0 CCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTAC GGGGGGGGFDFFFGDGGGGGGGGGGGGGGGGGGFGGGGBEGGGGGGGFGGEGGFEDGEDEFGGEEFFGEFGGBBGEEEEBEE:CD:AAAADEEEEE?CCC? PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:67:2263:13075/1 0 chrI 935 60 101M * 0 0 CCCAAATATAAGCAACCAAAAATATTTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTAC EDBEEEEEE?E?EEDDEEEEDDEECEDEEA>6C<@>BB>9?ACAAEECEE?CCEE?C:=B5AB??AEEEEB@DDD:>>@@C:?@??@:?@BCAC@@?A@?< PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:94:5592:18698/1 16 chrI 935 60 101M * 0 0 CCCATATATCGGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTAC ECECBEABA9AAEE@C;EDGGFEFGGGDFGFGEEEFFFFGBGGGGGFEAGCDGGFGGGGGGFGEFGGGGGEGGGEDGFGGGGGGGGGGDGFGFGDFGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:59:10637:1223/1 16 chrI 936 60 101M * 0 0 CCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACA CAEBDCA?EECEDDF@@GGGEGEEEEDEGEEFGGGFGEGFEEGGGGEDDE:FFFDACECDFEGFCGGFGFGGGFGAGGGFGGGBGGFGGDGGGGGGFGFGG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:70:4295:15837/1 16 chrI 937 60 101M * 0 0 CAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAG FCCBCB=CECBEEFEEGEGGEGEEEEGGGGEGEBEEEGGFDGGGGGFDDGBGGFFGGGGGFBGGGGGGGGGGGGDFGGDFFGGGGGGGG?FEEEEDBEEEE PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:64:5817:4431/1 0 chrI 938 60 101M * 0 0 AAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA GGEGBGDEEGGGGGDGGGDGGGGDGFFDFFGFGGGGGEGGEFEFDGGGGGDAFG?BEEBBFGFGGGGGGGGECGFGEFAEGGGF?FEF:A@?@C:?:@@@E PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:8:6755:19986/1 16 chrI 938 60 101M * 0 0 AAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGA BBEA==FEBAFFEFFFDADEE=D=CEE=D=;FFFDDFFFDEEEFFA=FB=FEFFFFEFAFFDDDFEFFDFDFFFF?DDFFFEDFFDFDFEFCDDECD:DAD PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:105:14800:10824/1 16 chrI 938 60 101M * 0 0 AAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTGGCTACAGA :GF=EEGGECDEFFGGFGGGGFFBEDDEF@EDGEEGCECEEE5EEEEFEF=FFGGDCGGEEGGDGGEGGGGGGGGGGDFGFGGFGFEB?EEE:EEDFFFFF PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:71:6398:5629/1 16 chrI 938 60 101M * 0 0 AAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA BCCAC:DFD?FF?>ECDDF?DEF?=FF?F?FFF?BFDFD?FDEEA=B??EE?E:B>@ACC5EEEEEDDBDFBDEBDEEDEDEEEE?ED:BECABECBBDA: PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:84:15268:14389/1 16 chrI 938 60 101M * 0 0 AAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA D:A@A?5:BCA?FCCCABDEEA@@5BFBAADEEDFFEBBBCEBEEED=5DEDDCCAFFFEDDADBD=5FFFDED=AEFDFFFDFFFF=FDDFAFFFEE:EE PG:Z:SNAP RG:Z:266-1 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:1422:86454 99 chrI 939 60 100M = 952 113 AATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA CCCFFFFFHHFHHJJJJJJJJJJJIJJJJIGJJJJIJJIJJJJJJJJJJJJJJJJJIIJJJJJHHHHHFFFBDCD@BDDDDDDDDEEECBDDDBDEDDDD PG:Z:SNAP RG:Z:000 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1308:20909:7788 73 chrI 939 60 100M = 939 0 AATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA @@@DFFFFFHHHFFHIIFGIHIIGJJJIGFGHGIGDGG9DGIJBHIJJIIHGHGHCG?A@BGDAECHFFDB;?CDDDD@CCDDC:CAD:?CB<AAA PG:Z:SNAP RG:Z:000 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1308:20909:7788 133 chrI 939 0 * = 939 0 TGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATT CDDDDC>D@@<>@ADCDEDEECCDBFFFHHHIIHHGBIIIGEHAIJJJHFJJJJJIHFFAJJIHCEIJIIGJJIIIIIHGHEBIIJJHFHHHFFFFF@@C PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2304:7438:34489 99 chrI 939 60 100M = 951 111 AATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGA CCCFFFFFHHGHHJJIJJJJIJIGJIJJJHIJJJIJJIJJJJJJJJJJIIJIIJJJJIJJJIJGHHGHFDFADDDBDDDDDDDDDCDDEDCDDDDEACDD PG:Z:SNAP RG:Z:000 NM:i:4 +EAGLE:1:25:14090:18624/1 0 chrI 939 60 101M * 0 0 TATATAGGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAA FCFFFGGEGGFGGGGGGGGGGGGGGGGFGEGGGDBEGGGGGGDEGGGGGGGGGGGGEFFEEEEFE?EEDEEC:EBECEEEEFGEDDFE?FBEE@DDDDD=F PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:33:5876:3688/1 0 chrI 940 60 101M * 0 0 ACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAA GDBGEFFGGDGGDGGGDFBG@GGFGGFEGFFGGEDGGFDEGGFEFBFFFBC?E=CB?ECAGGG=DGGFFFEFGFGGG??GE::55BCAEDED?EC5D5CC9 PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:55:14770:18631/1 16 chrI 940 60 101M * 0 0 ATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAA FEGBEEEGEEFG@GFGGFGGFGEGGEFGGEFGBGEGGDGGGGFFGGEGGDGGGGGGGEEGGGEGEGGGGGGGFGGFGGGEGGGGBGGGGGGGFDGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:93:9444:3777/1 16 chrI 940 60 101M * 0 0 ACATAGGCAACCAAAAATATTTTCCAAGTTTTTTTTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAA FFGADEGFFEGGEGGBGGFGGGGBGEEGGGGGGGGGFGGGGGGBGGGGDBFFDGGGGAEGGFGGGFGGGGGEGGGFGFGGGFFF=FDGGDGGGAGGGGFGF PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:50:5922:5719/1 16 chrI 940 60 101M * 0 0 ATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAACATCGTAGCTACAGAAA :???:???5:==8@EECCC5CA?5ECACCCEFEEEEEEFGGGGDEEEGEEEGEEEEEEEGGFGGEEFGGGGEGEGEGGGGFBAAD:GGGGGGGGFFGGFGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:21:17413:6903/1 0 chrI 941 60 101M * 0 0 TTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAAC EEBAD:E?DAFFF:FDDEBEGGGDGDGFEFGFGFGGFDDGEEGGGFFGGGGGDG?CDGGGFDFFGEEEEACEC=EEFGGFBG?G?FDGF=?A=D=FFF5F5 PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:120:7503:14055/1 16 chrI 941 60 101M * 0 0 CATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAAT EEDEB5GFEEGFGGGG:=GGFDDFFFAA7EEG:BBGGDFEGGADG=GGGBGGGGGGGEGGFGGGFGGFFFEGEGDGGGGFGEGGEGGFGGGGGFGGGGGGF PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:91:17352:13444/1 16 chrI 942 60 56M1D45M * 0 0 ATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACATAAATGG @CCBCDE?EEEEEEAEEEEEEGBEE>EEDEEGDGEBFEEEGGE?EGFFGEGFFFECFBEFGDFGGGGGGGGGGGGGFGGGGGDFGGFGGGGGGGGEGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:97:3188:16851/1 16 chrI 943 60 101M * 0 0 TAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGG :B??BB5=8CCEEEEADABDECB:EA@=?BB5C>C;:DAA::A=?D:ECCECEEEEC??C???=D?C??=?DB==?D?BDD:E=AEEEEAEDC?C?DDDDA PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:85:10558:18446/1 0 chrI 946 60 101M * 0 0 GCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAG FGGGGGGFGGGGGGGGGGGGGGEGGGGGGFGFFGGGEFEGFGGEGGDGEFGGGEGDGGGCGGGFADEFFFFEBFEFBEFFEEGEGDBEEEDGEEEDDE?=A PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:35:15009:8527/1 0 chrI 946 60 101M * 0 0 GCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAG GGGBGGGGABGG@EGGGGGGGGEGFGGGEGEFGGCGEGGGFEGGEGEFD?FGC=CEEGGEEDFE?DDFEFAACE=ECCCC?GDEEEEBBCEE@EECCB?CB PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:62:14360:14144/1 0 chrI 946 60 101M * 0 0 GCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAG GGGGGGGEGGFGGGDGGGGGEGFGGGFGGFFFGDGFEEFFGGGFEFGEGGGGEGEDGGDEFFFFADEFDEEEEED?EEEEEEGEEDEEEEEEEEEABB7AA PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:81:13994:10137/1 0 chrI 947 60 51M1D49M1I * 0 0 CAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCAGGTTTTTTGAAATGAATATCATAGCTACAGAAATGGTTGTA EEEEEB?EEDBFFFFFFFFFFBFFFFFCCCABCC5?DDD?FEEDFEFEFDBFEEFFFFFDFFEEEEFFEEBEEEEDDEBEEEEEEEEEEEEEEEE@?@D?@ PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:69:16589:8289/1 16 chrI 947 60 101M * 0 0 CAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGT @A???;>BA@DB:DA=BBB@@AEEDEADEEEEFBEFDFDDEBFEEEAAEBB?FEDFFFEFFFDFFDE:FFFEF?FFFDBFFF?EFDEEEEEE=?DEFDFFF PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:46:11875:10933/1 16 chrI 947 60 101M * 0 0 CAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGT =EBDDGEGEGDFGBEEGBEGGGGDFDGAGGGDGGGEEGBGGGGGGGCE?EEAD=D?GGGFGGGFFDGGGGGGFFGGFGEFGGDGGGDFGGFFGDGFGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:70:7204:17965/1 0 chrI 950 60 101M * 0 0 CCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACA GGGGGGGGGGGEGGGFBFDFGGGFFDFAGGGBEGGGEFDFEGEEGEGEEE@EFFFFGGGEEGGEEDBDEEEEEEDEEGGEEAB=AEEE@EDD?D@?B9@B= PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:47:16300:14806/1 0 chrI 950 60 48M1D53M * 0 0 CCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAATGGTTGTACAC GGGGGGGGDGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGFGGGGGGGGFGGFGGFGGGEEGGFGGGGGFFDFEGGEGFGDGEGGGGGGGEBFAECC?EEA PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:72:3307:18932/1 0 chrI 950 60 51M1I49M * 0 0 CCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAAAAATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTAC EEDEDDFFEEBGGGFGGFGGGDFGGFGEGGGEDGGEEGEGDFFGGEEGGEGEEFGGGAGFFADFFCEB?CEEDEDDCBCEBDBCCCECEACCACE;??;:: PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:55:6957:9011/1 16 chrI 950 60 101M * 0 0 CCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGAAACGGTAGTACA :A??BB?AAB=?=BBBBCCDC?BDDDBCDBBCDB?DB?:DDADDDDDD@?=DADD?DDDADDDDDDCDD:DDBD?:DD?DD?DDDDADDD?ACA>ADDD?: PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:41:5354:20962/1 0 chrI 951 60 101M * 0 0 CAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACAC GGGGGGGGGGGGGGGFGEGGGGGGGGGGGGGEGGGGGGGGGGGFGGGGFGGGGGGGFGGBEEGEFB=EEEEBED=BBCABEBEBEEEEEEAAC<=A<:@AA PG:Z:SNAP RG:Z:266-1 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2304:7438:34489 147 chrI 951 60 1S99M = 939 -111 CCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTAC #DDEEEDCADC@CDDDDDDDDDCDDDDDDDDDDEDEDEEEDEDFJJIHD@IIJJJIJJJJJIIGJJIJJIFGJGIIIJJJJJJIHGJHHHHHFFFFFCB@ PG:Z:SNAP RG:Z:000 NM:i:4 +EAGLE:2:48:13688:1594/1 16 chrI 951 60 101M * 0 0 CAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTGCAGAAATGGTTGTACAC @EEEEE?BEEEADBAC:EEEEEEEEEECEBEECEEEBEEDEEEEEE>>A@=BFDEFGGEEFGEEFEFGGFEFFGGFGGGGGDGGFGEFFFFAFFBFGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:14609:5903 165 chrI 952 0 * = 952 0 CTTCTGAAAATACAAAAAAGTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTT CCCFFFFFHHHHHJJJJJJJHHJIJJJJJJJJJJJJJJJJJJJIJIJJJJJJJJJJJHHHHHFFFFFFEEEDDDDDDDDEEEDEEDDACDDDDBDDEEEA PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2106:7309:94230 165 chrI 952 0 * = 952 0 CTTCTTAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHHFFFFFEEDDDDDDDDEEEDEEDDCDEDDDDDDEEEE PG:Z:SNAP RG:Z:000 +EAGLE:2:94:15425:16067/1 0 chrI 952 60 101M * 0 0 AAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACT FEGFGGGGFFFGGDGGGFGGFGFFFEGDFFEGGGDEEDDGGFDFGGEEGGDEGFGGGGBDEFFFFEBEEGBEEEEEGEEEEEEFEGDEEEBE;EE?BCCCC PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:14609:5903 89 chrI 952 60 100M = 952 0 AAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACAC DEEEEDDDDDDDDDDDDDCDDDDCCDDDDEEEEDEEFFFFFHHJJJIJJJJGJJJIJJJJJJIGHJJIHGJJJIJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:1422:86454 147 chrI 952 60 100M = 939 -113 AAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACAC CEEEEDDDCCCDCCCBDCADDDDDCDDDDCDDCEDDDDCCDFHIHHCHJJJJJJJJJJJJJJJIIJJIGIJJJIHIJJJIJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2106:7309:94230 89 chrI 952 60 100M = 952 0 AAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACAC CEEEEDDDDCCDDDDDDDCCCDDDDDDDDDDEDEDEEDEEDFHJJIEIJJJJJIJJJJJJJJJIIJJJJIJJJJJJJIJJJJHJJJJHHHHHFFFFFCBC PG:Z:SNAP RG:Z:000 NM:i:4 +EAGLE:1:48:14890:12005/1 16 chrI 953 60 101M * 0 0 AAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTC CEDF@C@EE=EEDEGEEBGEEEEDEBEFB?BEAEE?FDCC>CFFDDG=GEGFCGGGGGFEGDBGGGGAFGBFGAGEGEGFDFGGGGGGGGFFEGGFGGGGE PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:67:18282:4840/1 16 chrI 953 60 4M1I15M1D42M1D38M1I * 0 0 AAAAATAATTTCCAAGTTTTAATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCT >A9EBCDAD@@EFDEFBDFGFGEGGFEEE?EGFFGGEECCC@?:BAFBFDFGGGFGGGGGFADFGGDGDGGF?FFFDFFDFEEEDDBEEEBFFBFFGEGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:50:13587:7527/1 16 chrI 953 60 101M * 0 0 AAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTC GEFEEEEGEEFEEBDGEDGDFGGGEEGFGGGGBEGGFGGGGGGGGGGGEGFGBFGGGGEGGGBGGFFGFGGFGGFGFGFGGGFGGGFDGGGFFFFFEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2204:13467:2490 165 chrI 954 0 * = 954 0 TTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAGTATCATAGCTACAGAAACGGTAGTACACTCTTCTGAAAATGCAA CCCFFFFFHHHHHJJJJJIJJJJJJJJJJJIJGIJJJJJIJJBFIIJJJIHHHHHH@DFFFFFFEEEEDEDDDDDD@BDCDDDDDDDDCDCDCC@>::>C PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2210:13891:35020 165 chrI 954 0 * = 954 0 TGGAAATTATTTTTGGTTGCTTATATTTGGGCAGACAAAAAGTGTCCTAGCTAAAAAAATTGCAACTTTTTTGTATTTTCAGAAGAAGAGTGTACTACCG EEDEDDDDBDBDDDDDCCDDEEEDCBCCCEEFFEDFHHHGHIIJIJJJIIGJJJJJJJIGGGGGFIIEJIGDGJIIIIHEFJJJJIIHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 +EAGLE:2:71:10251:4806/1 0 chrI 954 60 101M * 0 0 AAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCT GGGGGGFGGGGGFGEGGGGGGGGGGGGGGBGGFGGEEFDECEGGGEEEGGFGGGDGCCFEFGEDDEEGEEEEEEEEEDEE@GEEECDCDD?CC>CCAACCE PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:2204:13467:2490 89 chrI 954 60 100M = 954 0 AAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTATCATAGCTACAGAAACGGTAGTACAATC ::>C@CA>:CCCDDDDDDDDDDDDDDEEEDCFFFFFFFHJJJIHCJJJJHJJJJJJJJJJIIIJJJJJJJJJIJIIJJJGHJIIJIHHHHHFB=24=+B= PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2210:13891:35020 89 chrI 954 60 100M = 954 0 AAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTC CCDC@A@@DDDDCDDDCDDCDCDDDDDDDEEEEDEEFFFHHHJJJJIJIGJHGJIIJJJIJIIJJJJJJJIJJJJJIIJIJIJJJJJHHGGHFFFFDCC@ PG:Z:SNAP RG:Z:000 NM:i:5 +EAGLE:2:50:6355:12073/1 16 chrI 957 60 101M * 0 0 TAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCT ?C=@CA=EDCDBEGEEGEEAEDEBEFDFBCEGEEGGGGGGEEFFEEGGFGGGGGGGGGGGGGGDGGGGFDGGGGGGGDGFGGGGGFEEEEEFGFFGGEGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:65:16283:15152/1 16 chrI 957 60 101M * 0 0 TAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCT EEEGEEEEBBDGGEGGECEE:BFGGGFFGEGFGEGDC?EEDCEEEAGEAGGGFGGGGGFGGGGGGGGADGGGFGGGGFFGGGGGGGGGGGEGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:79:12640:7363/1 0 chrI 958 60 101M * 0 0 ATTTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAACGGTTGTACACTTTTCTG FFFEADDDDD?=DD;A:::C=ADDBB?DCDEDFFD?EDEEAAEEAFDFDD=C?:CD?DBAEBEEEF=FAAEEDEE=CB=BBEBBBDBDD?ED@B@D@ED@: PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:65:16789:19099/1 0 chrI 959 60 101M * 0 0 ATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAATGGTTGTACACTTTTCTGA EEDDDCB?DBACCCCCC>EEEEEAEBBDDBEAEBCDEEDACEEEEEEEEEE@EEEEEBBEE?EAEEEEEBEEEEEEEEEBDEDEE@BDBB@B@@BBD@>=8>=A@@@AE@==BA PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:56:5751:3283/1 16 chrI 960 60 101M * 0 0 TTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCT AA@ABA5AFFFFBEEGDDE=FEF?AF?EEDA>>@>EEFAFCCCCC=DEG?FGEBFAGGFBGDDC5C5DFFD=GDGGEEEDGGGGBGGGGFFGGGDFGGAEA PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:7:4422:19468/1 16 chrI 961 60 101M * 0 0 TTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAA GBEEE?DBFFFGG:GGEGFCGGDECBFBFFD@@>D5ECEEAGEFGGGGGGAFG:GGEEEEEAFFFDGGGGGEE=EDGGGGGGGFGGFFFBFGGEGGCCGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:24:3469:6979/1 16 chrI 962 60 101M * 0 0 TCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAA EBB=BDGFFGFEFAF:GGGEGFAFEBDDEGGGGGFG=DDDGGGGGGGEFGCFFFGFGBGGGGAAFGGDGFGEGFGGFGGGGDFGFFFBFFAGGGGGGFGGD PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:107:10805:16122/1 16 chrI 962 60 101M * 0 0 TCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAA DA?C=EFABFGEC=DBEFFCFCCBBCE=ECCCCEFE:FAFGGGEEEEBDDEGG:EEFGEFG?AFFFFFFEF=FGGGGGGEGGGGGGFAGGGDGGGGGGBGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:103:2608:5570/1 16 chrI 962 60 101M * 0 0 TCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAA GEFDEBGFGGGDEFFCDEDEAGGFBG=GGGGCCFDEDFCBFGGGGGGGDGGGGGGGGGGGGGEGDGGGGGGGFGGGGGGFGGGGGDGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:103:8177:14669/1 16 chrI 963 60 101M * 0 0 CCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAAT EGBFCEEFAFEEEEEGGFEFGEEEEEGFEGGGGEEGGEEGGGGGEFEEFGGFGGGGGGGGGGEGGEGFGGGEFFFFGEGGGGGGGFGGGDGFDGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:77:9271:19697/1 0 chrI 964 60 101M * 0 0 CAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATA GDGFDFGGGEFGFGGGGFGGGGGGGGGGGGGGGGGGFGGGFDGGFFCFGFGGGFGGFFGGGGEGGEGGGEDGFEEEEFFDAEFCDGEGBEEGEEFEEEGEG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:88:12652:14437/1 0 chrI 964 60 101M * 0 0 CAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATA EEDEBEDEDEF=FFBFCFEAFFBFFFBFDF?DFFCE@=FDBBFEEC?BEAFEBEBFEEEEDEEFEBBEF@BE?ABAEDDB5==6=<@?@@@@=@B@A:AAE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:66:2841:12985/1 16 chrI 964 60 101M * 0 0 CAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATA EBEEGGG=DFEGGEFDGGGGGFEGGFDFGGCFFE?FFGAGGGGGGGGGGGGFGGGGGFGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:47:10826:17209/1 16 chrI 965 60 101M * 0 0 AAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATAC EDDDDBEEGEEEEGEFEGEG?EEFFFEEEGGFECGDGBEGEGGGGGFGGGFGGGFGGGGFEGEFFGGGGEEGGFGGDGGFGFFEFE5GGGGGFGGAEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:42:13795:17461/1 16 chrI 965 60 93M3I5M * 0 0 AAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAA C?CFDFBGEEFE==AA>>AB4=AABCBAB+<3BB7AAABAAB78)9)3=BAABABBAAAA9;?9;;.;>@?B=;=??>:=199=9 PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1316:21338:23882 89 chrI 1001 60 46S53M1S = 1001 0 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTCGNGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCN ##############################################@;C<-0(#??)CDECDBBDDDD PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1305:10647:87203 133 chrI 2547 0 * = 2547 0 AAAAACACATTTTTTGGAAAAAAAATTGTCCTCGAGGATCCTCCGGAGCGCGTCGAATCAATGTTTCCGGAACTCTGAAAATTAAATGTTTGTATTATTG @DCC@C@DDBDDCDEDDDDDDDDDDBDDDDDDDDDDDDDDDFFHHHIJIIJJJJIJJJJJJJJJIIJJIIIFJIJJJJJJJJIJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 +EAGLE:1:84:3615:2746/1 0 chrI 2728 60 101M * 0 0 TTAAATTATTGTAGAACCCTTTCGTTATTGAGATTTGATAACTTTTAAGTAATAAAATTTTCGCAGTAAGACATTAAAACATTTCACAATTAAGCTGGTTC GGGGGFGGEGGGGGEGGGGGGGFGFGEGGFGFGGGGGBGGGGGGGGFGGEEFAGDGEEGGGFEGGGEGGEGFGGEEGFEEEEEEGEGGEEEBFDD?@68?; PG:Z:SNAP RG:Z:266-1 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2110:10822:7981 73 chrI 2900 60 100M = 2900 0 TCCCAATATAAACGATGTCAGTTCTGCGACATGCTACAGTCATCCACTAAAGTAACCCGAATACCGACAAAAGAAGAGGAACGCCAACTTTGGATAGACG CCCFFFFFHHGHHJIJJGIIJIJJJJJJJJJJJJJJJGIIIJJJJFHJJJGIIHIJJJJJJJJGHHHFCAEDDDDCCDDDDBDDDDDDDDCCDCCDDDDD PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:2110:10822:7981 133 chrI 2900 0 * = 2900 0 GGCAAATATTATTTAAAATCAATCAAAATATAAAAAATAGTTCGTTCGCTGAAAATTTAAGCGTTTTCCGACCAAAATCAGCCCCTAGAGCGTCTATCCA CCCFFFFFHHHHHJJJJJJJJJJGIEHIJJIJJJIJJIJIHJIJIIJJJJJJHIHIJJJIJJIHIHHHHFECDBD?BDDDDDDDDDBDDDDB<@BDEDCC PG:Z:SNAP RG:Z:000 +EAGLE:2:60:6530:16775/1 0 chrI 2909 60 101M * 0 0 AAACGATGTCAATTCTGCGACATGCTACAGTCATCCACGAAAGTAACCCGAATACCGACAAAAGAAGAGGAACGCCAACTTTGGATAGACGCTCTAGGGGC GGGGFEGGGGGFGGGFFGGFGGGGGGGGEGEGGGGFGGGEGFFEDGGFGGGGFGGGCGGGEEFGEEGGGDBEGEGGEEEEEEBG?EGGBEFEEDDEEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1204:4496:9204 99 chrI 3991 60 100M = 4394 476 GTGCGCCGCGACTCCGCCCACCGGTGGTAACTTTTTGTGGGGGCATCTTTGTTTTTGGTCATTTTTCAGCGCTTTTCAGCGATTATTGACCAATTTTGAA ?=??;=@DDA1:?DBD:D::?@0;-;;-==7=C>>C8?A;>@8'3<5>AAAA????82?3>>3:>A>AA>3085<:>:>>5-28<>B>AAA<2>AAA>04 PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1204:4496:9204 147 chrI 4394 60 27S73M = 3991 -476 TGAATTTTTTTAAAACATTTTTAGTAAAAAATTGTTTTCGTGCAAATTGTCTTCGATATTATCCAAACGTGACGTTTTGCGATTTTCGCGCTAAAATTAC ###########################C>@A?2?;?B@@C>(>C@7;;@@;>?C;@@C;=73@;8FF:=D80)8F:@B;819IHFBDC@EE:EEB?=DCBCC;DED=EDDCEAEECAA?GEAGGGGEEGBEEEGGEGGEGGEGGGEEGGEGGGFFFGGEFGDFGGFGEGGGEGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2315:14704:45455 99 chrI 5186 60 100M = 5287 201 AACCAGCACCTCTGTTCTCGACCAACTATGATCATCATCGTCGAATAACCGTTTCTCGTGATTTGTCACATTATACTTGAGCACAATACATCCACCAGGT 5;5(@3.@############################################################################################ PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:2315:14704:45455 147 chrI 5287 60 100M = 5186 -201 TCAGTCCTTTCTGAAAATGAAAATTAATTTTAAAAAAATTGAATTATTTTAAATGAAACAGTTTTCAGAGATTTCTCAACTTTTGAGTCCCCCACCAGGC >8?A@BA:4(>AA>;5;:(:5A>>A@;>>;==6C==?=4HE@.@;FB@=4ECGGFB8=>B9*/910<@>B@>B?39DDCAGEHGC8?CEEEEEEEEEEEEEEECCDEFFGEEEEEEGEEGGGGGGEEFGFEGGGGFGGEGEFGGFGGGGGGGGGGGGGGGGGGGGDGGGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1315:14029:3494 73 chrI 12099 60 39M1D61M = 12099 0 AGTATTGTATGCACTGTATCAAAACACATTTTCATCTTTCTAGGTATTCAACTTCACGTTTTTCTGTAATAAATTCTAAATTCTTACCACTTTCGAGCAT CCBFFFFDHHHHHJIIGIIEIIJJJJ?BFHJJIEIIIJJJJHJJ?DFGHIGGIJJJIIBHIIIIGIJIGHGGHIJJIIGHEIIEGHHHGGHFFEBCCDDD PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1315:14029:3494 133 chrI 12099 0 * = 12099 0 AAGTTTTTTTTTCCTTTTTTTTTTGGGACGAAAAAATTTGCAAAAAAATTTTCCCGTGGTGGAGAGTTGAACTCAGACAAAGCCTAAGCCTAGGCTTAAT CCCDDFFFHHHHGGHIJJFIJJIJBEH=@>AC@DDDCCCDDD@CCCCCCCCCCCCCA>:CC@CCACDDDCCDDB?@DDDDCB@@BDDDCFFFDHHHEA;JJIGFHGJIHF?JJJJIIJJIJJHFJGHFJJJJIIJIJIIJJFFHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2203:20505:85498 73 chrI 13826 60 100M = 13826 0 AAATAATTACATTCAAATTAATATTTTGTTTACGGCTATGGCCTAGAAATCGCGTGGTGGCCTAGGATTCATTTGCGCGCGAAATTCAAATTCAGCCACT CCCFFFFFHHHHHJJJIJJJIJJJJJJJJJJJJJJJJGJJJBHJJIJJJJJJJJJJJJJJJJHHHHHHFFFFFFEEDDDDDDDDDDCDEDDDDDDDCBDC PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2203:20505:85498 133 chrI 13826 0 * = 13826 0 ATCTTAAAGAAGGAGTATCGTCAATGGGGAAGTTGTTTTAAAATGCAGTATTTGTACTCAAACTTTCAATTATTGCAAAAGAAAAAACGAAAAAAATCCG CCCFFFFFHHHHHJJHIJJJIJIIIJJJJHGIGHIHIIJJJJJGHIJJIJGGIJJJJJJJJIHIJIJJIJJJJIHFHHEEFFDDDBDDDDB@@BBBDD@9 PG:Z:SNAP RG:Z:000 +EAGLE:2:66:16575:12892/1 0 chrI 13844 60 101M * 0 0 CAATATTCTGTTAACGAATATGGCCTAGAAGTTGCGTGGTGGCCTAGGATTCATTTGAGCGCGAAATTCAAATTCAGCCACTTTCGTCGATTTCAACGGCA GFDGGEBGFGAEGBEGGGGGGGGGGGEGDGGFDGGGGFGBFFDFEGGDD?FGBDFGAGFGGGGEEGGGGGFBDGGCGEGEEEBGDGAB??BBBBB@ADADD PG:Z:SNAP RG:Z:266-1 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2109:19111:33831 73 chrI 13924 60 100M = 13924 0 CTTTCGTCGATTTCAACGGCTAAATGCGGAATGTTAACGGATTTTTTCCGTTTTTCTTTTGCAATAATTGGAAGTTTGAGTACAAATACTACATTTTAAA CCCFFFFFHHHHHIJJJJIIIJJJIJIJJGIGIIIIJIJJHJJIJJHHHHHFFFFEEEEEDDEDDDDEED=CDDCDEDDDA@CDDDDDDDDDDDDDEDDA PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2109:19111:33831 133 chrI 13924 0 * = 13924 0 AAAGTATAGCACGGCCATGTGTCGATTTACGCAGTTCGTGTACTCCTCGAGGAAAAACTTGGATTTATTTTTAAAAAGGTTATTACAGCTTTAGGTGAAA @CCFDDFFHHHHHJJJJJJIIHHIGIJJIJGIIJFIJJIJGIJJJJJJJJJJJJHGHHFFFFFFEEEEEEEDDCDCCDDCDDDDEDCDDDDDDDDCCCCC PG:Z:SNAP RG:Z:000 +EAGLE:1:88:5129:6620/1 0 chrI 13927 60 101M * 0 0 TCGTCGATTTCAACGGCAAAATGCTTAATGTTAACGGATTTTTTACGTTTTTCTTTTGCAATAATTGGAAGTTTGAGTACAAATACTACATTTTAAAACAA FFFBDEAEEEGGFGFDFEFFGDGGGGDBGGGADEEEE:DEEECE:EEBEEBFFACDD@FBEFDEBBD=:EEAEEDDD@DBEEEEFDBBDDED?EBDEEGEE PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:5:16715:13757/1 16 chrI 14162 60 101M * 0 0 ATGTATTTTAACACAGTTGTGACGTCATAAATGTATTTTAATACATTTTGCAACATTACTTGAATAACCCCATTAAAAATCAACTCAAGCATCAAAAAAAT :BB?EEEEEBEEEEAE=EEEEDEDEEBAEEEEEEEEEEEEEDBEBEEEE5EEEBE?E=EDEEEDEBADDDAEE?EDEEEEEEEEEEE=EEBEEEEEE?EED PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:28:17761:20206/1 16 chrI 14335 60 101M * 0 0 CAATTTTTTGTTTTTTGGTCCAACATTTTTTTTTGGTCTCAGCTCTGCTGCCTACCCTAGAAGAACTAATAGCGCTTCAAAAACTGATGAAAACGTTCAAA C:E?EEC?C9DDFGAEAEEFGEEEEGGCE?FBGGFF=GEFGCEDEE5DCAEFEEFGGGFGGGGGGGEGGGGGGGGGGGGGGGFEFGFGFFGGGGGGGFFFD PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2316:19241:55169 165 chrI 15544 0 * = 15544 0 GGCTTAGACATAGGCTCAGATTTAGGAATCTTCAAAAAATTGAATTCTAGATCTACATTATATTAAGCTGTCTAATTCTCTTCGAAAATTGTCGATTTTT CCCFFFFFHHHHHJJJIJJJJJIJJJJJJJJJJJJJJJIJJJIJJIJJJJIEIIJJJJJJJIIJJJIGGIJJJIGEHIHHHHHHFFFDEEEEDDBDDDDD PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2116:4460:94851 165 chrI 15544 0 * = 15544 0 TTTGTACTGTACCGCTACAGTAATCCGTTAGTATCCTAACATCTCTATACTTGAAGTTGAGATGAGTTGTATCTTCACAGACTACAAACTATGAACACAT @CCFFFFFHHHHHJJJJJJJHHGIJJJIJIJGIIJJJJJJJJJJJJIJJJIIJIJJHIJIJIIJJJHIJIIJJJJHHHHHHFCDFFD>BEEDDDEDDDD: PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2316:19241:55169 89 chrI 15544 60 100M = 15544 0 AAACTGCGTGGCGTGTACTGCAGAAAACCTCATATTTAGGCCCCGCCTTTTTCTCGACCACTCACGGTGAAAAGGCAAAAATTCGGGGACCAACCAATAT ADBBBBDBDBBBDDDCADDCDDDBA?9DDEEEDCCDDBDDDDDDFHEIIEIJJJIHFHDBHGGHJJIJJJJJJJJJIJIIIHGIIIJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2116:4460:94851 89 chrI 15544 60 100M = 15544 0 AAACTGCGTGGCGTGTACTGCAGAAAACCTCATATTTAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATTTGGGGACCAACCAATAT DD@DDDDDDDDDDDDDDDDDDDDBCB?DDEEEEDDDDDDDDDDHHJJJJJJJJJJJJIHFIIIHIJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:2101:3253:53534 165 chrI 15545 0 * = 15545 0 GTTGAATGTTGATAGCTCTTCCGAGCGAGTTTTTTTAAATTAAGCTTCATAGCTGTAACATTTAATATGAAGAGTTACACTCAAAATAAAAAAACTGCGT ?@@DDFDDFHDHGGEEFFIHHIGGIIEGBDEHJJIICBFHIDEC;CGDEEDA@EGAEECH;CEEDDEFBC@CCA3>CCDD@CDCDDC>ACDDB####### PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2101:3253:53534 89 chrI 15545 60 100M = 15545 0 AACTGCGTGGCGTGTACTGCAGAAAACCTCAAATTTAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATCCGGGGACCAACCAATATC A8DDB@9-?@?:CCCCDCDDDCB@89?DDCAACCC?@DBDDDDFEDEHHBGEIJJIIHEFDIIIIIGIIJJJGFGGGJJHGFJIIJIHHHGHDFFDD@@B PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2302:10568:99340 73 chrI 15561 60 98M1I1M = 15561 0 CTGCAGAAAAACTCATATTCAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATTTGGGGACCAACCAATATCAGGTCGCCGACATCTC =B@FFFFFHH+0AEEEHHAHHHCGHEIDIJGIGIIGGEG=F?HGFHIGIB@AEEHHGFBFDDAC2;>C(9;8;?BB?<@CDA>@4:<50&0>>@@>: PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2302:10568:99340 133 chrI 15561 0 * = 15561 0 AGAAAAGGCAAAAATTTGGGGACCAACCAATATCAGGTCGCCGACCTCTCACGGGTTCCGCGCGCCGCTATGTTTAACTCGCAGCGGGCGGAGACAGCTC A>AAAA@:B@>:BA8298<4(8@??:BEDB>43?5;00B@@;3+8(9?20&<:855'?@>DFIFIIIIFGFB?GFB)D0IFEIEFEEA?FDF?D;DD@@@ PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2205:19343:78160 165 chrI 15566 0 * = 15566 0 TTCTCAACGGTGGTAGAATTTTCACAGAACCGTGCCATGATTCAGATTTTGCAACCCGCGCACAGGTCATGTTTGAACAAATGTTGTCAATCCCTATAAT CA@??829BCCC@::DDCDDEDCDCC@??BBDDDDEEEECDFDFECHHECHGE@HGGGGGGCCIGGF>IGGJIHGIJGHGHEEIIJIFHHHHFFDDF@CB PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2205:19343:78160 89 chrI 15566 60 64M2D3M1I32M = 15566 0 GAAAACCTAATATTTAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATTTTGACCCAACCAATATCAGGCCGCCGACATCTCACAGGT DB@:3DEEDCCDDCD@DDDDB>??5?3C;;@>@CCBC>?BBADCCECEECCFFDEFEHHHHHHGEGIGGBGGIIIIIHGGJIIFJJHDGHGHFFDFFCB@ PG:Z:SNAP RG:Z:000 NM:i:8 +EAGLE:2:5:10071:18909/1 0 chrI 15576 60 101M * 0 0 TATTTAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATTTGGGGACCAACCAATATCAGGCCGCCGACATCCTACGGGTTCCGCGCGCC ECEEEFFFFFGGGGGGGGGGGGGGGGGGGFGGGGGEG?GGGFGGGGFGDBFFGGDGBGFGGGFG:EFDDFGGGGG=BF=FB@:D=BABACBEEGBBEC:E? PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1214:9682:34070 73 chrI 15598 60 61M1I1M37S = 15598 0 CGTCCACTCACGGAGAAAAGGCAAAAATTTTTACCCCAACCAATATCTGGCCACCGACATCTCACGGGCTCCGCGAGCCGCTGCGTTAAACTCGCTGTGG @@@FFFFFHHHDHIJJJIJCHGGF@FFGGHIJI<@D=FGGEHGGIIHI>FEFC-@;':B8CDE##################################### PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:1214:9682:34070 133 chrI 15598 0 * = 15598 0 AGTTTTTTTTTATTTTGAGTGTACTCCACGAGGATAATTTTTATAATTCTCCTCCTCTTTTCCTCGAGGAGTACACAAACTCCGTGAATCCACACATGAC ##DDDDDDDDDDDDDDCDDDCC@B@BDDDFFFFFFFHHHHHJJJIJIJJJJJJJJJIJJIJJJJJJJIHGIIHCHIJJIJIJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2101:5512:59159 165 chrI 15648 0 * = 15648 0 GCGTGTACTGCAGAAAACCTAATATTTAGGCCCCGCCTTTTTATCGTCCACTCACGGGGAAAATGCAAAATTCGGGGACCAACCAATATCAGGCCGCCGA :?BD=0=AFHHGHIIJJJJIJIJJJJJIEHGJJJIIIIIJJJIJJJJJJIHGIIJIJHHFCDEEEDDDDDDDDDDDD@BDDDDDDDDDDEEECDBB@DD# PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2101:5512:59159 89 chrI 15648 60 9S91M = 15648 0 AATATCAGGCCGCCGACATCCTACGGGTTCCGCGCGCCGCTATGATTAACTCGTTGTGGGCGCGGCAAGCTGTCTCCGCCCGCTGCGAGTTAAACATAGC #########B>5BDCAC>:B?599>B@93BBBDDDDDDCDDDDDEDC@?DDDDDDDDDDBDDDEDFFFDDEEBIGIIJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1216:7173:80911 99 chrI 15656 60 96M4S = 15842 286 ATCCTACGGGTTCCGCGCGCCGCTATGTTTAACTCGCTGTGGGTGTGGCGAGCTGTCTCCGCCCGCTGCGAGTTAAACATAGCGGCGCGCGGAACCCGTG B@CFFFFFHF@DFHIII1C6DDFHBHGHJHEHIHHCBEFAEFF9AACBBBDB@=A>A>@C<5@B-5>BB9095@>CCC@AC4@B-0559>@CBB8BB-77?9::>C:(+22;++:?A99-09CC@<@@C:>A####### PG:Z:SNAP RG:Z:000 NM:i:6 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:17151:68349 133 chrI 15710 0 * = 15710 0 ACACCGCTTGTTTCAGGGCTGTCGTTTTTTCGTTTATTCGGGTTTTTTTTTCGGTTTTTAGAACCAAAAAAACAACAAAAAAGTTTGGTCTGTGTGAAGT ######CDC>>C?EHIGGGGGCHF=8A<7B;DDBBBDDDEDDFHJJJIJGJJJIJJJJIJJIJJJJJJJJIJJJJJJJJJJJJJIJJJJJJIJJJJJJJJJJHHHFHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:82:8204:15172/1 16 chrI 22312 60 101M * 0 0 GGGGGTACATGGACGAGAATTCTCTACCGTATTCCAATTTGGCTGACTGCGTGCTCAACGTTGAATACTCAGTGTAAACTTTCGTACACCGTTGCGTACTG E@EE@B@5BBB@@EE?BEB5BEDEEA?CEEE?DBEDEDEDEEE?EE?EEEEEEEEDEBEEBAEEEEBEEBEEEDDEDEDDE?EEEEDCDDDDAD=DC@CCC PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:2:88:6414:9094/1 0 chrI 23853 60 91M1I9M * 0 0 AAATCGAAAAAAATAATTGCAAAATCCGTCAGCTGAACATTCAAAACTTATCAATTTGAAATCAGCATATTTCAGTGTATAATTAAAAAAGGTTTCAAAAA GGFEFGEEDDDDEECFFFFFDFDDFFFFFFFEFFFFFFFEFFDFAAEEEEFFFBBDFFFFFF:FDCFF?FBFFEDEFEFFFFFFF?FFFFEA?BEFFFFEB PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:1:88:12428:19742/1 16 chrI 23928 60 1D15M1I85M * 0 0 GTATAATTAAAAAAGGTTTCAAAAATTCTGAGACCAATTTTTATTGAGAAAAATAATTTTTCGCTCGAATTATTGAATTTTCACTAAATGCAAAAAACAGT GBD?CGDEEEEFGFEFFD?FFFDFFDDEEAEE=EEEACCFE?GBFFDGDCGFFFBEDGFDAGGA=GFGFBGFFFGGEG?GDGA?DGEDEGEEEFFDEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:2 +EAGLE:2:39:4472:4563/1 16 chrI 23983 60 101M * 0 0 ATTTTTCGCTCGAATTATTGAATTTTCACTAAATGCAAAAAACAGTAAACTTGGGCCCATGCTACAAGCCTGAATCTTTCAAATTAAGAACCAGCATGATT 6=CCDDGEEEEEEEGEEFGEEDEE?DEEGDGDBGEEGGGEGDGGGEFFCFEAGGFGGGGEGFGGFGFGGGGGFFDGDFDEGFFFDEGFGGGGGFGEFGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:48:8251:3932/1 16 chrI 23995 60 101M * 0 0 AATTATTGAATTTTCACTAAATGCAAAAAACAGTAAACTTGGGCCCGTGCTACAAGCCTGAATCTTTCAAATTAAAAACCAGCATGATTTTTTCAATATTC @DDBD????@==>@>8::FEEEGE@EFGEEGEECACBBCEGEGFGDFFFFBGFEBGFGGGGGFGGGGDBEGEEE5EGEGGGGGGGFGGGFGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:2 +EAGLE:2:96:5841:4603/1 16 chrI 24002 60 101M * 0 0 GAATTTTCACTAAATGCAAAAAACAGTAAACTTGGGCCCATGCTACAAGCCTGAATCTTTCAAATTAAGAACCAGCATGATTTTTTCAATGTTCTAGGACG EDBE=EBE?BBEFAED<<AAAEA?BB:DDDADDED?DDDD=DDADDEDEE?EBDEEEEEDDEE5C:EBEE?E=EBBEBE>CEEEAE=?DEDEEBEBDEEE=?BEEACCEEEB PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1209:7257:71539 73 chrII 202 60 100M = 202 0 TAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGCCCGTTCACCAAAAATAGTGACTCTGACCGTTCACTA @CCDFFFFHHHGHJJIIIIJJJJJJJIJJFAGGIJJJJJJIIJJJJJJJGIJJJICHIIEIIJJJJGHHFFFFFAA??ACC;ADDDDDCCDCBDDDCDDC PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:1209:7257:71539 133 chrII 202 0 * = 202 0 CACTAAATTTGGTGAACGGTCATAGTCACTAAATTTAGTGAACGGTCAGAGTCACTATTTTTGGTGAACGGGCAGAGTCACTAAATTTGGTGAACGGCCA CCCFFFFFHHGHHHIJIGIFGHHIIHIIJJIJGIJJJJFHIJJJIHIIIHFFGHJIJJJJJJIJHIGIIHHGFDDDDACDDDCDDDEDDDDDDDDBDBB> PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2316:19021:74938 165 chrII 231 0 * = 231 0 CTAAAAATAGTGACTATGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCG CCCFFFFFHHFHHJJJIJJJJIHJJJJJJJJJIJJJBBCFHEHDFBHEHGHHIJIACFAHGIFFHI@GDGAEABBDFECEEDD??BDDCDCACCAC5:A< PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2316:19021:74938 89 chrII 231 60 100M = 231 0 TGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCCGGCCGTTCACTAAAAATAGTGACTATGACCGTTCACCAAAT CA>DB?BDDBBCCC@BAB?BBABCDDB?HHIGGHGHGGHC;GJGGHHIJJGHECAJJJJJIIJJJIHJJJJHFHHHHHFDFFFCCC PG:Z:SNAP RG:Z:000 NM:i:6 +BS-DSFCONTROL03:121:D0M8KACXX:5:1313:5017:58480 73 chrII 234 60 100M = 234 0 CTCTGCCCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTTTACCAAAAATAGTGACTCTGACCGTTCACCAAATTTA CCCFFFFFHHHHHJJJJJJJGIJHIJJJJJJJJJJIJJJJJJJJIIJJJHIJIGJIJIJJJGEEHHDFFFFDEBCEDDCCDCDDDDD@DDDDDDD@CDC@ PG:Z:SNAP RG:Z:000 NM:i:6 +BS-DSFCONTROL03:121:D0M8KACXX:5:1313:5017:58480 133 chrII 234 0 * = 234 0 CGGCCAGAGTCACTAAATTTGGTGAACGGTCATAGTCACTAAATTTGGTGAACGGCCAGAGTCACTATTTTTGGTAAACGGCCAGAGTCACTATTTTTGG @BCFFFFFHHHHHJJJIJJJIJ@FHIJJJEGIGIIGHIJJJCHJJJJIIIFHIJJJJJJJJDHHHHFHGFFFFCDECEDDDBBBDBA:ACDC>A@CDC<9 PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2113:7908:63038 73 chrII 234 60 100M = 234 0 CTCTGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTA CCCFFFFFHFHHFJIIIJIEHIGEEHGIIIJIJJJJFJIIIBGHIIJIJDDIGJJJJJJJJIIHHFGHHBDDEEEBDEEEDCA@C?@BDDDDDDAACDCC PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2113:7908:63038 133 chrII 234 0 * = 234 0 GTCAGAGTCACTATTTTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGTCACTAAATTTGGTG BBBFFDFDFFHHHJJJJIJJHIJJJGIIJJIIIFGGHIJJJJIJJJDGIIHJIIGIIHHHHHDDDFDDEECC=ACCCBB8;BDDDACDDCCDDDACACBB PG:Z:SNAP RG:Z:000 +EAGLE:1:59:19240:17867/1 16 chrII 234 60 101M * 0 0 CTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCCGGCCGTTCACCAAAAATAGTGACTATGACCGTTCACCAAATTTAG =?CCCC:5@?:CA56;7???:EEDFAGEGEGGECFEAFDF=GFGBFGGGEGBGGGGEGGGGG=GGGFEGGGGGGGGGGGGGCCCCC7DDDDFGGGGGGFGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +BS-DSFCONTROL03:121:D0M8KACXX:5:1203:6884:43459 73 chrII 238 60 100M = 238 0 GACCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGA @CCFFFFFHHHHHJIIJIIAHHIIJJ@HHGIGHIJJJJGIGIJDHIJIJIGGIJJJIIIJEIJIEHIGHHGHFDEEFFACCEDDDCDDDD@DDCD@>@CC PG:Z:SNAP RG:Z:000 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1203:6884:43459 133 chrII 238 0 * = 238 0 GTCAGAGTCACTATATTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGTCACTAAATTTGGTG ?@BDBABAFFHHHJJJJJJJEDHIJJGIJJHGICGHIJJJJGIIJJIGIJJIJJJJGIJHHEEHEE@@CFEF=AC>CDDDDD??B:@ACAACDAAAAC?? PG:Z:SNAP RG:Z:000 +EAGLE:1:50:4361:1823/1 0 chrII 240 60 101M * 0 0 CCGTTCACCAAAAGTAGTGACTCTGACCGTTCACCAAAAAGAGTGACTCTGACCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGACTC GGEGGGGGGGGGGGGGGGGGDAFFFDFFFFGGCGFEGEGGFC@ABFEEFEGEGEFCFGAEEEDE:DD@>CC5@ACAC:CCC:C==?A?@=:;;;=@CA?@5 PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:85:17281:13087/1 16 chrII 240 60 101M * 0 0 CCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTC ==<76>CF@CAGEDEFEEC?EED?CDBCB:=EDEGGGGCDEFFEE?FFFDFDGGFFFGEFFGFEDFFGGDGGGGEGGGGEGDDGGGGGGFDGEGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:78:10927:5210/1 16 chrII 240 60 101M * 0 0 CCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGACTC F@?@:B;EEEE:D?EEDEEAFEFEEEFFDCEGEDAEFAFDFEDFFDFFFFGGFGGBGGGDGGGGGGGGGGGEGFFGGGGGGGGGGBGDGGFGGGGDGGEGG PG:Z:SNAP RG:Z:266-1 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:1116:20571:27060 165 chrII 241 0 * = 241 0 AATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTCAGTGACTCTGGCCGTTCACCAAATTTAGTTACTCTGGTCGTTC @@BFFFFDHHDHGIJJJIIJJJJJJJJJJGHGIHGIJJJIIIJAHIJJJJJIJJJJIGGHIDAHIAHIIHFDDFFEEEBCCDCDDDCDCCCDCDCC==???@>>>@?:EBB?:B:B@EEBBEA?B@?@>8@@>@@;?>? PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:55:11493:15098/1 0 chrII 245 60 101M * 0 0 CACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGGCCGTTCACCAAATATAGTGACTCTGGCC 5FFFFFFFD?ADBDDFFFAE=?DDBFEFFFAD?F;DDDADEEEEEFABC=B?EEEFDBFE??DAA:?@=B?C=@B>5=@@;8;87>>=>>B?5BAB6@::9 PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:61:8991:6659/1 0 chrII 246 60 101M * 0 0 ACCAAATATAGTGACTCTGACCGTTCACCAAGTATAGTGACTCTGGCCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGGCCG FGFGGGGAGGEDGGGGGGEGGGGGFFAFFFFF:FAD=@CDEEE=EFFED=AAAABFBG:BBBEAEEAEFE=?EEE=>=>>GDDEDDB@D@=A@@?A:BBB9 PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:73:10783:17479/1 0 chrII 249 60 101M * 0 0 AAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGGCCGTTCACCAAATATAGTGACTCTGGCCGTTC GEGGEGDGGGAFFFFGDGGFEGGGGGGGGGFFGFEFFGGGFFGGGDBGGGGGGEFDGBGEBEBFDFCD?ECECDCBDFDEEED?EC@BBEEEEEEEEEEEEAEEE?E?AEEEEEBDEA?C@>@=EB<8BEEBDDBDBC???CC5@B??@?EBEBEDEE@A:@?B:?=@=@:B@B@???BB PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1316:6280:52543 99 chrII 1371 60 100M = 1443 172 GCTCAGGACACCTCATACAACTCCAGAGAAAATGTGTCTCATTATTCTTGTCTTTTTTCAAGATCTAATCAATTTTCTACATTAACGACGTTTTTGTCGT CCCFFDDDHHHGFGHGGJIJGGGGGIHIGHIJIIJI?FHJIJIJJJIIEIGEGGEHIIJAFC@CHHG>EHH@CEFHHHCDE@C@3;A59;8DDC?>:>9DBBBEFEDFEGIIFJJIHA:IJJJIIGF>GIIGIGIHFC@FFGFJIHDGH?GHFHHFFFDDFFCC@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1302:4189:8113 99 chrII 2989 60 100M = 3262 369 AGTATGGACAATTGTGAACGGAGAGGTTCAATGGAAGACTCCACCGCGGTAAGTGTGTTTCTTTAAAAATTACTTCCTTTTTTCAATTGTTTGAAATTAA CC@FFEFFGHHGHJEIGIJJJFHAHICGFGIIGIGHGGGHHFHIIJJIJ4@EGEDAEECHHFFFEBDFEEEEEDDDDDDDC@BCCDEDDDDC?CCCCDA@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1113:11974:31213 99 chrII 3014 60 100M = 3127 213 GTTCAATGGAAGACTCCACCGCGGTAAGTGTGTTTCTTTAAAAATTACTTCCTTTTTTCAATTGTTTGAAATTAACAAGAAACCTGTTGGAGCGTATTTC CCCFFFFFHHHHHJJJJJJJJIJJEHIJBGGHHIIIIJJEHIIFIJIJIIJJJJJJJJHHFHHHHFFFFFEEEEEECDDDDDDDDDDDDDDDDD9BDDED PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1113:11974:31213 147 chrII 3127 60 100M = 3014 -213 CGAAAATATCATTTGCAAAAAAACTTGAAAATTGAGAAACTTTTTTAAAAGTGGAGTAGCGTCTGCGGGTTTTTTTGCCCTAAATGACAGAATACATACC BEEEEEDCDDCDDDCDDDBDDA>DDDEDDEDDEDDEEDDDDFFFFFFHFHHFJJIJJJJJJJJJJJJJJJJJIJJIFJJIIIJJJJJHHHHHFFFFD@CB PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1302:4189:8113 147 chrII 3262 60 4S96M = 2989 -369 TGCGATTTTTATTTTTATTTTTCATGAATGTTAGGGGCAAAAAACCCACATGCGCTACTCCGCCTTAAGAAGAATCAGCTGTGAGCACTATCCACTATAC ####?CCC@C?:@DCDBBCC@>CCDDCC@A<A;?BBB;5EEECIHBA@9ED?@0FDBGFIEHIIJIGJHGJJIJJJJIJIHHDHGHHFFFFF@BB PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:87:12598:13225/1 16 chrII 4380 60 101M * 0 0 AAATTCATGAAAACTTTACTGAAAAACGAAGACCCATTCGATCATGCAAACAAAATATAAGTTTCTATGAAATGGACGGGGATATAGAAGAATTTGAAGTG DAAA?CCDACC?=?CABCCEACD@C?3CDCDCACC@;EC?=ACDDDDBD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1104:15150:63415 147 chrII 8021 60 100M = 7998 -123 GAGAAAGCGTGTAAGACCATGAAATGTTTTTTGTGTGCGCAACGTAGTGCGAAAAGTTAGTTATTTGGAGAGGAAGTTATTTGAAAAGAATCTTTAGAAG CCCBBDD@DDDEDCDCDDEEEDACBDDDDFFFHHHGIIGIHDIIJJIGIIGHJJHIIIJJJJJJIIJIGHFJIHEIJIJJJJJJJIHBHHGHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2314:19499:38834 99 chrII 8057 60 100M = 8275 318 GCGCAACGTAGTGCGAAAAGTTAGTTATTTGGAGAGGAAGTTATTTGAAAAGAATCTTTAGAAGATATTGATATTTTATATTCTGTATGTAGGCGCTGTG 7BBFFFDFHDFDHEHGGIAHGHIJDHIIJJJJBGFHI;DHBGIJJJGHIJIIJIIJJJJII)=EHFHHFFFFFFFFEFDEEEEDEDDCDCDCAA?@BDBC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2314:19499:38834 147 chrII 8275 60 100M = 8057 -318 GTTCGTATTCTCTCAACTCATGGCACTACTCTCGATGTGCATTGCCTTTTATGGTTGCTACGTTATGGTTCCTCTTGGAAGAGAAAAGCATGCTCCATAC BDDDDDDDDDDDDDDCCEEEEECCFEB;FHHHHGDGJIJJIJHGIIJJJJIHCJIHGHHFJIJJIJIJIIFIIIJJJJJJIIHJIJJHHFHGFFFFF@BB PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:79:2735:13063/1 0 chrII 9267 60 101M * 0 0 TACACTATTCTTAACTTTTTGACATGAATTAAATCTTTTTAAACTGAGTCTAAGCGTATTATTGTATCGTATTTTCCCCTTCTGATGTATTCATTTACTTG ADEBED=ADC=C6=CD=DD6C5BA>A?DDDEBE=AACCA<@B:EEEAEB?ED=EEA PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2114:17065:72325 147 chrII 9559 60 100M = 9394 -265 ATGAAAAGCCTGTTCTTTGGTGTTTAAAGGTAGCACACAAACCAAAATTTGTTAGCGCAAATTTAAATTTCTATTTCAGTTACCATGAGTTAGTCTAACA @DDDDDB?@DDDBDDDDDDDFFFFFFHHHHHJJIHGGJJJHJJJJJIGIHJIIJJJJJJJJJJIJJJJJIEIJIJIJIJJHFJIIIJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:91:5280:21105/1 16 chrII 10935 60 101M * 0 0 CAATGCCAATACAAAATAAGTCCGAACAGCCAGGTTAACAACTTGAGTATAAACCAGAGGAATCGGCACCATATCGAAAATGACCAGATTTAGAATTTTTG 8==?=AAA??589??A8A5A?BAA?>D?ADAABABADAEE?EBDDBEBEGEBBFFD=:?;5ABEBEGEFFEGEBDGFEFFGGBGGEGGFFEGGFFFGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2209:12690:32871 99 chrII 11660 60 100M = 11873 313 TTGTTAAGCAATATTCTATAAATCACTGATAAAATAGAATAACACAAAAGCCAAATTAGTAACTCTGACCATATTGATTTCCAAACCGATCCTTTCCATC CC@FFFFFGHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIIHHHJIJJJJHGIIIJIIIIJJJJJIHGIJJJGGHHH=BFDDDDDDDDDD@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2209:12690:32871 147 chrII 11873 60 100M = 11660 -313 TTGTATCAGTCGGTGGGTGAGCGATTAGTATCAAGTAGCACAAACGCTTCCGTTTCAGATTTGCATATTTTATTGTTATAGGGGTTATTCAGGCATAGGT DDDDDDDDDDDDDDDDDDCEDFFFFFFHHHHHHIIIJJJJJJIJIIHG@JJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:2:118:13551:9082/1 16 chrII 13278 60 101M * 0 0 TGATACTTATTCCAATTTCATTCCAATTACATTCATGCTTGGATTTTATGTCTCTGCTGTTTTCACACGATGGTGGCAGATTTTCGACAACATAGGGTGGA EBCDADACBC:EFDEBDFEBEDEBEF=EBFEEBFDEEFEF?FFDFFAAFEFEDGFDGFGGFEEE?EEGEGGGEGGGGGGFGGGBBGFGDGEGGGGGGFGGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2107:16551:14335 99 chrII 13632 60 100M = 13690 158 TTTAGTTACTGCTGGCTTGATGACAGAAAAAGAAATGGCCGAGTTCGAGTCTATCCCTAGTCCACACGCAAAATATTGGCAGCCAATGCATTGGTTGTTT CCCFFFFFHHHHHJJJJJJJJIIJJJIJJJJGGIJGIJIJJGIFHIJJJGHIJJIJJIJJHIJHHHHFFDDDDCDDDDDDDDBDBCCDCCDDCDCDD9BA PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2107:16551:14335 147 chrII 13690 60 100M = 13632 -158 AGTCCACACGCAAAATATTGGCAGCCAATGCATTGGTTGTTTTCGATGATCACTTTAGCGCGAGACGAAGGAATGATTTCAAGTGATATCATATATGTAG CCCDDBB?DDDEEFDDCDDDDDEEEEEFFFFFHHHHJJJJJJJJJJJIIGHFJIJJJJJJJJJJIJJJJJJJIJJJJJJJJJJIJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:47:2491:18712/1 16 chrII 14238 60 101M * 0 0 GAAAAATTTGATTATTTAATTCAATTTTAAGTTAGAAAAATATCCTACACATTTTCTGAAGAAGTGTCGTAAATGGGGAAACTTTTTAAACATGTACGTTC EEEBCEAGEGEGBFEFFEDEDDEFGDEGDGGGGEGEGDFFCEFFFFDFDFFEBEEFBFGFGGGGGGGGGGGGGGGGGGDGEGEGGGEEGGGGDGFAEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:57:14985:11088/1 0 chrII 15505 60 101M * 0 0 ACTTTAACTCTCCTGTCAAATACAACACTGATGGGATGAAAGACCGAGAGCTTCAAAATCCAACACCAATCACTGATCACATTGATTTGCCTTTGCATGTG FFFFFFADFFDAA?DEEBEEDFFFFFDFFFFFFFF?EEEEFFFFFF=F?BFBEDDBBCBBFEFEDFD?=E@@E@B:=??CDD:EDDBB5>>=5=>B??B?C PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:21:7517:15303/1 16 chrII 15804 60 101M * 0 0 TTCAGGTAGTGATCTAGGCAAGTCTGGAAAGCGGGAGAGAAAGAAGAGCGAGTGATTTTTGAACAGTATGATAAAATATTTTTTGTTTCTCTTTTCACTCT E5AFEBAFDEDEFFCEAEC?ED?DAEFFCF?FFFFFFBFFFFDDFEDDDD?FADFFF=DFFFFFFEEF=FFFFFFFFFEFFC>FFFAEGFEFDBDFBDDEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1212:2979:94901 99 chrII 16035 60 100M = 16399 464 GTTCTATAGGACCCCCCCTAATTTGAAGGTTTGAGGAACTTCCGAAAATTTTTTTGAAAAACTGCTAATGCCATTCGTTTTTAAATTGAAAAAAACCTAT CCCFFFFFHHHHHJJJJJJJJJJJJJIJJHIJJHIJIIJJJJJJJJJHHHHHHDBDDDDDDDDCDDDDDDDDDDDCDCCDBDDDDEECCDDCBDDDD?C@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2310:11814:52578 99 chrII 16055 60 100M = 16433 478 ATTTGAAGGTTTGAGGAACTTCCGAAAATTTTTTTGAAAAACTGCTAATGCCATTCGTTTTTAAATTGAAAAAAACCTATATACATTTTTTTCCAGAAGT 1=BD=2=ADCFHHGJJJJJJJJJJJJJJJIJIJJJIJJJIIIJJJIJIJJHIJJJHHGHHFFFFFDEEDEDCBBDD@CCDDDEDEEEEDDDDDDECCCDC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:74:13479:19610/1 16 chrII 16067 60 101M * 0 0 GAGGAACTTCCGAAAATTTTTTTGAAAAACTGCTAATGCCATTCGTTTTTAAATTGAAAAAAACCTATATACATTTTTTTCCAGAAGTTTATCTCAAAAAC EEEFBEECBDDEEEEEEEEFEEEEEEEEEGEGGGFEEGFEFBEFGFGEFGGGGGGGGGGGEECEE?GGGGEGGFEGCCDADD:DDDGGGGGFGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:60:16490:10079/1 0 chrII 16083 60 101M * 0 0 TTTTTTTGAAAAACTGCTAATGCCATTCGTTTTTAAATTGAAAAAAACCTATATACATTTTTTTCCAGAAGTTTATCTCAAAAACTGAGGTCGCGCTGGAA GGGGGGGGGGGEGGGGGGGGFGGGGGGGGBGGFGFFGGGFGGGGGGGGGGGFDFGGFGFGGGG8GGGEEGGC?FGDGDGGGEGGGEGEGGBDEGEBAECCG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1313:14692:64885 99 chrII 16208 60 100M = 16423 315 CTTCTATAGGACCCCCCGTTTTTTTTCACGATTTTTACTAAAATCAACAGATTTTGGAATTTTTGACAAAGCTCAAATCAAGTTTGAGTTAGAAATGAGT CCCFFFFFHHHHHJJJJIIJIJJJJJHJJJIJJJJJIJIHHHHHHFFFFFCEEEEEBDDDDDDCB@DCCCDDDDDDDDDDDDDDEDDDDEDDDDCDD>CD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1212:2979:94901 147 chrII 16399 60 100M = 16035 -464 AGTCTGTTTCAAGCTTCCTGGTGCTCTCGGTAATGCCAAAACTTGATAAACTCTCTTTAACAAGTTCCTACTAAAATTCCTAGCACACACACCATAAACA ABDDDDDCBCDCDDDDDDEDCFFFGGHGIJJIIEIGGCJHDB@IIGIHEGIGIIIIJIJIIGGHCIHCIHJJJJJJIJJIGIGGEIHHFFCHFFEFDB@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:79:2445:14024/1 16 chrII 16410 60 101M * 0 0 AGCTTCCTGGTGCTCTCGGTAATGCCAAAACTTGATAAACTCTCTTTAACAAGTTCCTACTAAAATTCCTAGCACACACACCATAAACATTTTTACGCCAT CACAACF=CCDEG@GEFDEAEE:EEFG5EEGEFEFEGFEGEGEFFEGGGGGGF=EGGEEGEDGGFFEFFBFFFFFFEEEAEGGBGDEGGGGGFAGGGFFEF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1313:14692:64885 147 chrII 16423 60 100M = 16208 -315 TCTCGGTAATGCCAAAACTTGATAAACTCTCTTTAACAAGTTCCTACTAAAATTCCTAGCACACACACCATAAACATTTTTACGCCATCCCCAAGAAACC >DDDCDDDDDDDDDEDEEEEEEECB?B>FHHEHGHGIJJIHGJJIHIGJJIIIIJJJIHHDIIIHHGJIGEJJIIJJIJJJIJJJJJHHHHHFFFFDBCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2310:11814:52578 147 chrII 16433 60 100M = 16055 -478 GCCAAAACTTGATAAACTCTCTTTAACAAGTTCCTACTAAAATTCCTAGCACACACACCATAAACATTTTTACGCCATCCCCAAGAAACCAGTCAGAAAC CDDDCDC@:@DCDAA>DCEDCDED@CBCEHCBHHE@EBIJHFAGHGHEEC;ECGFFDJIJIJIIIEIGGHHEIGIHGEJJJJJJJIHFHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:84:7059:2112/1 0 chrII 16465 60 101M * 0 0 CCTACTAAAATTCCTAGCACACACACCATAAACATTTTTACGCCATCCCCAAGAAACCAGTCAGAAACAGCGTATTAACAAGTTGCAGTTATTTTTGATCA GGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGFGGGGGEGCGGGFGGGGFDGEGGEGGEEGGGEEEEEEDFCD@EEADDEEEEBECE>C@BCCFDCFEHIIBDEI99:C06DB<4?BBBB<@?67B=AAHG/AAC:?DFDDF(66>>@######################## PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1104:21126:70246 147 chrII 16691 60 35S65M = 16655 -101 CATTGATCTGACCAAACTTTTCAGCTGGTTGTCCGGAATAGACTTCCAAGCGTCGAGAATTCCTTGCTTCAACGATGCAACTGTTGGGTAAGTCTTGTTC ###################################?C=)?7).):;@CAA@EHIJCEHBHFFBC3;ACCECCCCC@CDACACD> PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:75:15288:18925/1 16 chrII 16992 60 101M * 0 0 CTTGCTACTGACGAACTGTATCTCAAGCTTCTTCTTCTCCGTGAACGCTCCCCAAACCATCACCGTTCCTCCTCCAAAATTACGTCTCGAAAAAACCATTG CC@CDD?DDDAEEEEEEDDBEFDEAEEGBEGEEGEEFDGGEGFGEGFGFFFFFFFAEEEEECGFGGEGGGGDFGGFGFGGGGGGBGDGGGGFGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:48:9783:12656/1 16 chrII 16993 60 101M * 0 0 TTGCTACTGACGAACTGTATCTCAAGCTTCTTCTTCTCCGTGAACGCTCCCCAAACCATCACCGTTCCTCCTCCAAAATTACGTCTCGAAAAAACCATTGG =???=7A???A5==>:A??=A5BA?@BBB?B:BAAA5?A>:BA=BBBABBD?B?BACBCDBDCDCBDDBCDBDDDDD?DDBDD?DC>>B==DD=ADDD:DD PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1312:16439:9749 147 chrII 17198 60 100M = 16889 -409 CACTCTTGCTTTTTTAAATTCTCACTTTACTCCAATTCGTTCCCATATTGTTCTTAGCAAATTCCAATCGCTTGAGTTTATGGTCTGCAGAGAGTAACGG 4@DDDDDDDDCDDEDCEEDCCD@;@CDDEHHFHFIIIJIGIJIJJJJJIJIHIJJIJJIJJJIJJIIGJIJJJJJJJJIIJJJJIJJHHHHHFFFFDCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2310:10135:61245 147 chrII 17321 60 100M = 16930 -491 CGATTACACCAGATCGTTTGATGACATTGAGGATGGTCCTTTTTGAAGCAGACAATTGAAGCTCATTGCGAATATCTCTTGCCGTCTTACAGGAGTTGGA 0:@@@AA:CCB@DB@DDCCDDEDCCCEEFFFFDHHHHEBIJIHJIIGIGEGAGEJJIIJJIHGCHBGHGIGIIIJIIIIJIGJIJGGFHHHHFFFDF@B@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1301:10889:93999 99 chrII 17346 60 99M1S = 17605 341 ATTGAGGATGGTCCTTTTTGAAGCAGACAATTGAAACTCATTGCGAATATCTCTTGCCGTCTTACAGGAGTTGGAGGCAGCACGAATCACATTTCGTTCG @?@DDDDABFHCFEFHIIII=HHCFGHGABBE3@FFH=CBCFEGGAAGIGHHEE>5@@B@C@CA?B=?AB;;?B<:@:A>3+8??# PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1116:3353:52872 99 chrII 17407 60 99M1S = 17466 159 TTACAGGAGTTGGAGGCAGCACGAATCACATTTCGTTCGTCACGCACGGAGAGAGCTTTGCGACGAGGAGCTCTTTTAGATGTACCGTAGCTCACCGGAT CCCFFFFFHHHHHIJJIJJJJJJJJJJIJIJJJJJJJJJJJJJJJGIJJJHHHFFFEEDEED@BDDDBBDDDDDDCCDDDDDDDDDDDDBDDDDDDDBD# PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2211:10693:67001 99 chrII 17455 60 100M = 17585 230 GAGAGAGCTTTGCGACGAGGAGCTCTTTTAGATGTACCGTAGCTCACCGGATCCTTCAGATACTCGCGAATACAGTGTCGAGAACGGGAAATTTTCCTAC @@@DDDDFFHHGHJJJGIGJIGGIEGHIJIGGGDDBGHIDHIIGIIGCAEEHFFFFBCCECCCCDDDDDDDDDDCAADEBBBDDDDBDDDDBDDDDCCCCDCCA:CDDDDBBCDDEEDEEDFDHHHIIGGIJJJIHGFGIGJJJJJJIGJJJJJJJJIJJJJJJJJJJIJJJJIHHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1307:19886:52946 99 chrII 17486 60 96M4S = 17520 134 ATGTACCGTAGCTCACCGGATCCTTCAGATACTCGCGAATACAGTGTCGAGAACGGGAAATTTTCCTACTCATTTCATGCAGGGACACATTGAGCAATTT <@@FFDDDCCFGHIIIIGFH6EFGGCH>@H(5>:>:>@A>;5;>(:48&8@?BC>3>C>CC#### PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1307:19886:52946 147 chrII 17520 60 100M = 17486 -134 GCGAATACAGTGTCGAGAACGGGAAATTTTCCTACTCATTTCATGCAGGGACACATTGAGCAATTTCATAACATCCAGCTGAGCGCGTTCAGTGTCCGAA >C@4:@C@CC;@9C;CB?DCDCEEAHEHHA@CCC88;GHCIIGGGA>@4C@AC>CCAB>5B=3,(@>@@CDCFFGHHECIHGECCIDGF@:DEG@BFGHGDGAGGDFD4FBCEGHGB@EGGHGGEFGIGIHHHHHDAFFFC@@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1301:10889:93999 147 chrII 17605 60 18S82M = 17346 -341 ATAACATCCAGCTGAGCGCGTTCAGTGCCCGAAAGGGCAGATCCTCGAGGCATTGCAAGTTAGACTGCTTTCGAAGTAAGCTTTCCAGCCTCTATATGTG ##################?CCC?A;9''ECA?7=DC=)';7''**8/9@BDB9@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2112:17571:72038 147 chrII 19888 60 100M = 19694 -294 CAAATTGTTATTTATTTCCGATTCATCTTTATAACTTCAATAATTTATTTGAGAATTCTATTCAATGCTATAAAAGTCAAAAATTTGCGAAACGTGGTTT ADCCDCCDDECC>AACDBFEHEFGHCD:EGGDHHFA>@IHGIJJJGGGGGGHEHFAEHIIGGJIIFF@GGD@IIJIGGIIIGHGIJJHHHFDFDFFFCB@ PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:2302:11666:55394 99 chrII 21802 60 100M = 21978 276 CTTCTGAGAAACTCGAACTCATGATCAGAAACGCGCTCTGATCGAACATTCATACTCTAACACATTCCTCTTCACATCTCCAGATGATTATTCATGTCCA CCCFFFFFHGHHHJIJJJJIJJJJJGGGIIIGGIJIJJJJJJGIGHIJJDIGIJJJJJGDEEHHHDEFDFFEECEDCEACCDCDDCCDCCCDDD;3;A;5 PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:89:11611:13275/1 0 chrII 21896 60 101M * 0 0 TGTCCATCATTTTATCATTAAATATCTCATTCTATACTCTCTTCTCGCTTTATTGTTTCTCGCTCACTCCCTACCCACCATTACATAACATCTCTGAAATT EEEEEDEEDEEEEBEFFFBFEFFEEFFFEEGGGGGDGGEGGGDGGGGGFGGGGGGFGEGGGFEGGFGGGGGEEGGGEGE?ABBEEEEGEEEEEEEBEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2302:11666:55394 147 chrII 21978 60 100M = 21802 -276 ACATAACATCTCTGAAATTTCAAAGTTTTGACATTCTTGGCTGTGCCTTTTCCTCTCAATGATATTTTCCAAATTCCATTATTTTTCCCCCGCCTTTGAT 3CDDDEDCCA>3@DDDDDCCACDDDCCCDCDDDDDDDDDDDDBA??CCA@############################################ PG:Z:SNAP RG:Z:000 NM:i:7 +BS-DSFCONTROL03:121:D0M8KACXX:5:2203:12843:33593 133 chrII 24481 0 * = 24481 0 TCGAACTTTCTCGTGCAAAATCTCTGGCCTTTTCTGTATACTTTAGAATTTGAATTTTCATACGCATGGTTTTCTACACCAAATGAACACATCTGAGAAA @DEDCDDDBBDDEEDFFFFFFHHHHJIIGG>JHIGGIIHGJJJJJJJJIIJJJJJJJIJIGCJIJJJJJIIIIHGFGCJJJJJIHEGHFHHHFFFFFCCC PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2102:18771:39670 99 chrIII 1739 60 100M = 1876 237 CACCACAATTCAATACTTTTCAACTCTTGAGAATCGAAATAGGGCAGCGTAGATATAATATCATTGGGTGACACAAAAATAATCGATAATTCCTTCACAT CCCFFFFFHHHHGJJJJJJJJJGJJJJJJJJJJJJJIJJIJIIIJIJJJHFHIJJJJJJJJIIJJJJIEEHHHFFFFDDECEC@BDBDDDFACCDCCDDC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2102:18771:39670 147 chrIII 1876 60 100M = 1739 -237 TGTGGTAACGTTAAGGTCTTGTTGAGTTTGTACAACAAATTCGAATTTCCTGACACGTCCCAGTAATATTCTCAAATCGTTGAAAGCTAGTTGCATGAAG BDCCBDCCDDCDDDDDDEDDEFFDDFHHHHHHHJIJIIJIIIIJJJJIJJJIIIGJJIIJJJJJJJJJJJIHJIJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:51:14937:4579/1 16 chrIII 4052 60 101M * 0 0 CTCAAAAAAAATTCCAACGAACAAAATGTGTAGAACTTTACAAACTTTTTAATAAAGATTTCCAAATTTTTATACAGATTCACTAAATTTCCAGAGTTTTC EB7<CCFFBF=FFABEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2312:17962:78103 99 chrIII 19688 60 100M = 19760 172 CTGTAGAGACCCAGGCAGGCGAAACGGGTTTGGCAGAAGCCGCTTCGTTACGCGACAGAACCATCCCCCACCCGCTTGTAAATATATACGCAATCAAGAT @CCFFFFFFHHHHJJJJJJDGHIJIIIJ@GIGHHHICGC;FABEGEEHFFDDDDBDDDCCCDDDDABDDBBBDBB@@DBCDDDDEEEE@BBBBDDDDDAC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2312:17962:78103 147 chrIII 19760 60 100M = 19688 -172 CGCTTGTAAATATATACGCAATCAAGATTACGCAACGCAGCTGAACGAAAAAAAAAAAGAGAATGGGGGAATCTTTGAGGGGAGATAATTGAGAGGAAGG @DDDC@CEEDDCCDDBBCCDCCDCAADBBDBDBBBDDCDCADDDDCEDHIJJJJJJIJJIIIIHJJJJJJJJJJJJJIJJJJJJJJJHHHHHFFFFF@@? PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2109:9506:74934 99 chrIII 21761 60 100M = 22497 836 CAAGGATCTATGCATGATTTGTGGTAGATGTGCCTGGAAAAGGGGGATTTTTGAGTTGTTTTCTAGGTGTGACCAGGTTTTTGATATGATTTTTGAGAAG CC@FDDFFFHGGGGGGGIJIHFHIIE>B4<AAC>@AC@BB?>@>CDCCD>8<<>< PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2109:9506:74934 147 chrIII 22497 60 100M = 21761 -836 TCAACAAAAAAAAATCGAATTTGAAAACTTATAAAATTTATCCAATAAATGGGCGGAGCTTAGCCAGCTTTCTCAACAACTCACTTGCAAGGTAATAATC CACB<@;?EEA>EA@74JJIFCC;GHF:GIGIFBDGG@EGBDCA@>CAEHHGIJJJHHFFGFFFFD@<@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:41:8435:11353/1 0 chrIII 24117 60 101M * 0 0 AAATTTTGGTGAAAGTGGGCGGAGCCTAAGTAAAGGATATATTTTTTAAAAATTATCAAAAAATGTTGCAAACTTTAAAAAAAATTTTAAGTTTTTTTTCA GADGGFFFEEEEEEEEGGGGGGEGGGGFEG?BBCEFFFFFDGGFGEGGGCFGGFGGFGGEEEDEGEGGGFEEDEGGEEEGED@=6DD?EBGADBDDB=C?E PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:95:11679:7360/1 0 chrIV 621 60 101M * 0 0 ATTTCCCCTCAATTTTCTTGCAGATCGACGTGGATATTGACGACCTAGATGACGATTTTCTCTCCACAAACGGAATGGAACGACACGGTTCAGAATTACAA EF@@@EEEEEBBABBBECEEEEEBEBEFBDFDEAFEGD5DGGDEFGGGGDFGFG:DFFFFFGBGGGGGFGF?F:FEADBEEBEEEEBEBEEEE==:EAAEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:58:15136:13881/1 16 chrIV 723 60 101M * 0 0 GAGAAGTGGTTTTCACTACGACGAGTACTGCGAATAACAATGTGAAACGAGCCGTCGAATATTTCGAAGATGATAATCAAGATGATGCACTAACATCCACT @A9A9AAA??A?:?:??;@?:>>??=5A?A?@:C=CCADBCDDCFBAGEEEEFFGEFDEFFDG?GGFGGGFGDDGD?FFDGAEE=?EECDAFEEE?EEEFE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:25:10330:11791/1 0 chrIV 1025 60 101M * 0 0 TTTATTGAGGAATACTCTATTGAGTTGGACACCTCTGGGAAGTATGTGTTGGGAAAAAAAATCCGAAAATTGATTGAAAAATTCGAAAAAAAATCCAAAAA GGGGGGF=GGEGFFGGGFGGGGEGEFBFDFGGGDGGGFGFEFBFFEBEBEFFFEFFAFGGGEFGGEE=GDEDEBEGEDEDEFEEF@DEEEEG>CBBDDEBF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:29:6386:17433/1 16 chrIV 1252 60 101M * 0 0 AATTGATGGATAAAAATTCCAAAAAAGTTCCAGAAACAACATTTCCTCCGCAGCTTCACCTGGTCCCAAGTCTCCATTTGACGATGATTTTACTGATACAG E?===FBDGGFDEE=FE@GGFEEBFACAAD=FFFDEFEDGGFFEDFBAFGGFDFFF=BGGFFFDF=GDAGGG=GGEGFDGGGGEDGFFDFDEEEEDEEEDE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1214:19701:60229 99 chrIV 1474 60 100M = 1594 220 GTCCGGTAGAGACCCATTTGATGAAGATGTACGTTTTTCGTAAAGTTCGGAATTTTTAGGGTATAAATTGAAAAACAAATGGAGAAAATATGAATACCTA BBBFFFDDHHHHHJJJJJJJJBHIJJJJIGIIIFHIJIJJIJJIJIIJJJJIJJJJJJIJJDHHHHHHHFFFFFEACDDDDDDDDDDDDDEDDEEDDDDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1112:16947:43819 99 chrIV 1479 60 100M = 2024 627 GTAGAGACCCATTTGATGAAGATGTACGTTTTTCGTAAAGTTCGGAATTTTTAGGGTATAAATTGAAAAACAAATGGAGAAAATATGAATACCTAGATTT <@@DFFFDHHFHHIIIIIIIHI>FAFHIHGHEDHGCFHIG?F?DFHIIFIGHHGGG.=FEGFH>CDHGBHH2?@C??>>;AC@5@B3;5;>::>>>(:@@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1214:19701:60229 147 chrIV 1594 60 100M = 1474 -220 CCCAACCCTAATATCCACAATTTTTTTTCAGTCTGGAAACTCTAACGAAAACCAGCTCCGCGAGAAGAAGCTCCACAAAAAAGAGCAACTAGCTCACCGG BDBBCDCCDCCCDCBC@DDDDDDDDDDDDDDDDEDDCA;DDDDDBDFFB?=HHHIJIJJJIJJJJJJJJJJJJHFJJJJIJJJJJJHFHHHHFFDDFCCB PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1112:16947:43819 147 chrIV 2024 60 18S82M = 1479 -627 ATTAGAAGAGAAGAGGAGGAAGAAGTGGTGGAAGAAGATCCAGCAGTGGTAGTTCCAGTTCTACGGAAGGATCTGGAAGTTGAGGAGGCTCCGAAATCGG ##################CAA>;A;(>D?;>?HEA=74E:C=>@@;=8=89FBFDCGFD;69D:?AD?9?1*9<:9E??AA+;FGFGD<:8A;BA=+1=: PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2204:2799:95211 99 chrIV 2692 60 100M = 2824 232 TTTCAGACGTCTGTATCCGCCGATCCAAAATCTCACACGCCGATTTTGATGGCCGACGAGTACAAAGAACCACAGAAGCAAGGTGAGTTTAGTTCGAAAA CCCFFFFFHGHHHJJJJJJJJJJJJJJJJJJJJJJJJJIIJJJJJHHHHHFFFFDDDDDD8ACDDDDDDDDBDDDDDDDDACB:CCD>@CEDDDDDBDDB PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2204:2799:95211 147 chrIV 2824 60 100M = 2692 -232 CATTGTTAGAAAATTTGAAAAAAAAACCAAAAACCGTCGTAAAACTACACTTTTTCTATTGAAAATAGAACAAACAAATCAAATATCCTACATGTAAAAT :@4(DDDDDDDDDDCDDDDDDDDECDFFHHHHBJJJJJJJJJIGIIGHGJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJHJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2203:18040:19562 99 chrIV 2921 60 100M = 3122 301 AATTTAAATCGAGTGGAATTAAATTCTTCAAACAAAAAGAAATCTGCATTTAATATCGATTTCTCATTCAATTGTTATGAATCATAAAACATGATTTTTT CCCFFFFFHHHHHEHIJHIJJJJJJIJJJEHJJJJIIJJIJJJJJJJJJIJJIJJJJJGIJJJJJIJJJJJIIIHDHHHHHHHFFFFFFEDEEEEFEDD@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2203:18040:19562 147 chrIV 3122 60 100M = 2921 -301 ACAATTGGGATTAATTTTCAACTGAACTAGACTGTTTCTGAACGCTCAAAACCTTCAAAATCAATGCTTCTTCAAAACGAAAAAATTGCACACTATAAAC CDDCCCCCDEDDDDDDCCECACEEEFFFFFFFGHHHDJJJIIIIJIHIHFHJIJIIJJJJIJJJJIJJJJIHIJJJIJJJJJJJJJIHHDHHFFFFFCB@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:51:5632:6609/1 16 chrIV 3456 60 101M * 0 0 GATACTGCATTTTTCCGGAAAAGGACAAAGTCGGCTAATTGCCGTTTTTTATAATTTTTGCTCATTAAGCTTATTCCATTAAAAATACAATTCTTGTTTAA ACA;C@CDBCCEEEEEEEEEEEEEEEEEBEEGEEFGEBGEEGGEEEBEEDGEGFEFGGGGGFEGGGGFGGGGGGGGGGFGGEGGGGEGBEDGGEFGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:41:11958:20308/1 0 chrIV 3987 60 83M1I2M1I8M2I4M * 0 0 CTTTGAAAAATAGGGAATTAAATTTAAATAAAATTTTCAACTGAACTAGACTGTTTCTGAACGCTCAAAACCTTCAAAATCAAATGAAATATTCAGGAAAA GGGGEGGGGG?CCFFGGGGFEGGGGGFDGGGFEGGGGGFGGGGDGFFGGGGEGEE?FDFCGEEEEGGEEEEGEEEEEEE@CDDCCEBDDBEAEEEDBEEEA PG:Z:SNAP RG:Z:266-1 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2216:15913:7063 99 chrIV 4720 60 100M = 4891 271 TTCAAGAGCCTAAGTTTTTTTTTTCGAAATGAGTTTTTTAAAATTGATCGAAATGTTTTTAAATACTGAATATTTGGGATTCTAGATTTTTACTACAATA CCCFFFFFGHHHFIHJJJIJJJJJIJJJJIIJIFHIJJJHHHHHHFFFFFDEDDDFEEDDDDCDEDDEDDDEEEEDDDDDDDDEDDDEDDDDDDEDDDD> PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2216:15913:7063 147 chrIV 4891 60 100M = 4720 -271 TCTAAAAAATTTTTTTAGTGCCTGCACCAGTTGTCGTTGCTCAGGAGTCGCCGATCTTAAAACGAAGAAACTCTCTAGTACCATCAAGGATCAGTGGAAG CCDDCDDDDDBDDCDDDDDDDDDDDDDDDDDDDFFFFHHHHHJJJJJIHFJIGIJJIIJJIHIJJJJIGHJHHDJIJIHHJJIIJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:49:18520:10880/1 0 chrIV 6174 60 101M * 0 0 GATTTTGGATTAAAAAATTCCGAATAAAAATCAACTTTAATTTAACGAAATTAATTTTCTATACCTAATTCTCTAATTTTTGCAGAACAGACGTAACGATG EEEBEEDAA?DDB?ADEEEEEEEEEEEBEE>EEEDE?EB=EEEDBEE=EEEEDE5?ADBDEEEEEEEAEEEAEBE:DDBCDEE?EBDEDEEEEB:EEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1103:16672:84478 73 chrIV 6412 60 56M44S = 6412 0 TTTCGATATTCAACACTACCTGCCCAAGAAGTATAGCTCATTTGAAGATCAGTCACGGCTGAAGAACATTCTCGCCGAGCTGCACGGGCATTACGCGGGC 1==A1AA+<4,2>A3A3A7A@=?AABC?9AA:=<*7?*=*:7*0***9?79=ADA8677)7A7AED7;3??@#################### PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2305:20092:23229 147 chrIV 7551 60 9S91M = 7114 -528 CAGTACTCCTGTATTAATCCTACAGTAATCCTAGAGTGCCCCTACAGTACCCCAATAGTATTTCTACTGTACTCATTTACTCCTACACCTAGAGTACCCT #########C>>3@>>;)@@BDCA=)>?77@@CGEG@;F;94?/AD@?0BBDHHFHFB9BBB@FDFFHGIHGIII6B8=B8@=<;=DHHECCEACCB@@@:>CCCCC@CBBBBBC<5@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2213:7767:73428 147 chrIV 9538 60 100M = 9395 -243 TTGACATTTTTTTTGGGAAACATGAAAAAAATGAGCATGATTTTTTTTCTATTTTCGATAGACCTGAATATATTTCCGAAAAATTCAACCCATCCATCGC EEDCCDDDDDDDDDDCCEEEDEDDBDDEFEDDDDDDDEEDDHJJJIIJJJIGFIJJIIGGHFJJIJJJIIGIIJJJJIIJJJJJJIHFHHHHFFFDDCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2108:20580:46115 147 chrIV 9624 60 100M = 9443 -281 AACCCATCCATCGCTCACTTACTGTGAAAAAGTTCCACTGCAGCCCGAGATAGTGATGTTTTGTGGGCGAATAGTTGTCGAGAAGCGTTCGCATGTTATG 89<2AC?5BBB>A>@C@DDD@@:CCCCC@A@>A;>>;;?1DCBB?=;CC=4C7.)ADF@BDB;0>F?F?B@HDAHBD?CEHGGGFC@87HHD?ADFD@@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:45:8531:9371/1 16 chrIV 10605 60 101M * 0 0 TTTAAATTCAAAAATTTACATAATTTTTTAGTTTCTAGTGCCCTACGTTATTTCTCCTTCCGATAGAAATAACACTAAATCGACAAAACATTTTGCTCAGA :CAEFEDCEGFEDDEEDEEDEGEFEG?FEFDEF?FDCGEGFFGEFGGGBGEG?GGGGFDGGGFFGGEGGGEGFFGGGGEFGGGFGFGGGGGDGFGGGGFGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:45:5297:17573/1 16 chrIV 10799 60 101M * 0 0 AGAAACAACACAAAAACCGGTAATCATTATGGAAGTTCGTTGTGTCAAATTTGCACGATTAGTGAAAACGCTGATCTTCGATGCTTAAAGACTTCATCGGA EBBECBBDC@EDFDCCFBAEEEEBGFGGGGEEFFGFEAFDBGEFGGAGGGEGFGDGFGGBGDBGGEEGGGFGGGDDFBGFFEGFGGFFFFBFFA?F?GFGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:90:8728:2691/1 0 chrIV 11034 60 101M * 0 0 AGATCTGTGATAGAAAGACGAGGAGGAGACTGATCATTTTTTGGAAAAAATAGATTTAGTTAATTAATTAATTAATTTAAAAATAAGTATAAAAGTCGTCG GGGGGFGGGGGGGFDGGGGGEFFGGFEFGGGGFGGG:AGFGGEGEGFFGGEGEDGGBFGEFGEGGGGEGEDEEGEEEGBEEEEEEACAEDDDBDEADB?BD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:58:9805:10604/1 0 chrIV 11061 60 101M * 0 0 GACTGATCATTTTTTGGAAAAAATAGATTTAGTTAATTAATTAATTAATTTAAAAATAAGTATAAAAGTCGTCGTCTCTTCTGCCTCTTTTCGATTTTAAA GGGGGGGGGGGGGGGGGGGGGGGGFGFGGGGGGEGGGGGEDGGFFGGFGGGGGGGGGGGGEGGGGFFFCFFDFFCGDGEDGFEGGFGEEEDGE=DEDDDCE PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2102:2939:36410 99 chrIV 12069 60 100M = 12243 274 GAATTGATTAGTCAAAACCTTACAACTTGTGACTCAGAATTTTGAAAGTCTACCGAAAAAATAACGTTCTTCAACAATTACGATTCTTCAATTCTAATAG CCCFFFFFHHHHHJJJJJJJJJJJJIJJIEHGIGGGGIIJJJJIIJJGGIGHIIIJJIJGH2BEGH?EEFF>DEECDEDEC@B?ACCDCD:;ACDACCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:23:5518:6512/1 16 chrIV 12134 60 101M * 0 0 GTTCTTCAACAATTACGATTCTTCAATTCTAATAGGTTTTTTTTCCGAAACTTTGAGTTTTGCTACCTCTAAAATATGTGATTACAAATTATGGAAACGTA ?CBAC=CA@:AEBBBD=FCEEE?=EECBA?CBFDFC>:EE=:C:EEEEBEE5FAEF=EEEEDEEBEFBFFDFFFFFFBFEEFFBDFFADFF?DDDD5ACC= PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:9:17295:4977/1 0 chrIV 12165 60 101M * 0 0 ATAGGTTTTTTTTCCGAAACTTTGAGTTTTGCTACCTCTAAAATATGTGATTACAAATTATGGAAACGTATTAACAAATTACAACAGTACACTTGGTGTCA AEECDEEEEEDGGGGGFGGGFGGGFGGGFFDEEEEGGGGGGFG?GGAFGGGFGGGFFGGFGGG=GEEEE?E=EEEFFGDDGG?AGABAD?=CEBEEADBEB PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2102:2939:36410 147 chrIV 12243 60 100M = 12069 -274 TTACAACAGTACACTTGGTGTCAGAGTGTCTTATTTCGGCTACAAAAAATGCGGGTAAAGAAACGCAGAGTTCTCAATTAATTTCGCATGGTTAAGAACG ACCCDDCDDDAC>DDDDDDDDDDCCCDDDDDCDBBAFFEFFHHHJJHJIIIJIJJJJIIIHFDHJJJJJJJIJIIJJIJJJIJJJJJHHHHHFFFFF@CC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1211:1649:59300 99 chrIV 12490 60 100M = 12628 238 CTGCCGGCCGTAAAAAGTCATTCACCTCATCATCCGGCGATCTTCTCAATGCTCCAAACCGCACTAATGGACAATCCCGAACACCACCTGCTCCACGAAA CCCDFFFFHHHHHJJJJHIIIJIJJJJJJJJJJIJJJJIIHHHHHHFFFFFFEEEEEEDDDDDDDDDDDDDDDDDCCDDDDDDDDDD@BCDDDD@CB9@@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1211:1649:59300 147 chrIV 12628 60 100M = 12490 -238 GAGCAATCGGAGATGCTCTGCCGACTCTTGACGGCGATAATCTCATCAATATCAAGGCGATCTCGGGGCTTCATTTGGATGAATCTGATGAGGAAAAGGG DDDDDDDDDDDDDCAB?DBDDBC@BDDDDDFFHHIGJJJIJJJJJJJJJIIGJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:57:18691:3342/1 16 chrIV 13429 60 101M * 0 0 ATTCAATTTTCGGCAAAAAATAAATTTATGATGAACAAGTTGAGATTAAAATATAACTTTTCCTCTGATGAAATTTTTTTATTTTATCAAAAAATCGGAAA BBB=FDFFF?FAE;C?EGGGGGGFDGDGGDFFEGBGDEGGGGGF=GEGGFGFGGGDGGGEGDEGDGEEECDDDGGGCCBEAEFFDDDDDE=FFAFFGGFGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2304:10466:54597 99 chrIV 13926 60 95M5S = 14386 560 GTTCAAAGGCCCCCTCCGGCATACTCGGATCAGCATCATGCGAGTTCGACATCTGCTTCGGCTCCGCTGATATCGGTTATGCTTCTGAAGAAGCCGATGG C@@FFFFFGHGHHJJJIJJJJFAFGGGIGIIIJIJJEIJJJJFIADHGEHBDFEFFFEDDDDBDDDDDBDCCACDD?3CDDDDDDDCD@CDDDDDDDDDB PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1103:15940:55697 147 chrIV 14107 60 100M = 14066 -141 AACTTCGGCAATTGCCGGTTGCCGAAAATTTTGGAGTTCGGCAACTTAGGCAACTGCCGATATCAATGTCAAACATTTTGTAACCCATAAAAAAACATTC @><>(@>@C>3>ACDCA=;5BB=9=3?7;7CHHHHG?GIHGGGHDBJJJIJIGIHGEB?JHF?IIHHCFJJIGHHEJHEIJIGHGHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:45:7639:19200/1 16 chrIV 15054 60 101M * 0 0 CGTGTCAATACCGTTGCCTTATTCTCTTCAAATTTCTAATTTGTAACAATTTTCACACACACACTTATACAAAAATTACGGAAAACTCTCTTGAAACAACG C?BBBBBA@>EFEBDDBB=EABBFAED:FF=EEBBBAE;BE=E?EAEBDBBEBDBEBEBEBEAEBBFC=FFFFECDFDFFFBGGEFFGFGGFFFFEEECEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:32:13629:16208/1 16 chrIV 15294 60 101M * 0 0 ATACAATATTACTGTGATAGAAGCACTGAAAATTCTCTTTCATAAAAAAATTATGTAATGCGTTGTTGTTTGCGACAGACTTTTATTTATATATGACATTA DBDGGBGEFFEFFGGEDGAGGFGGEGAFAF?DGDBGGFGDGFGGGDGFG=GDGFGGEGEGGFGGGGGGFGFGGGGGGGEFGDGGGGGGGGGGGGGGGGGGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:18903:53675 99 chrIV 16174 60 100M = 16449 375 AGTATAGGTTGAGTGTCATATTCGAAAGTGGCCTCCACTCCTCCAAATATTAGGTTATGGATTTATCTGTGCTGTTTCACAAGAAATGCCCAAGCTTCTT @@?DBDDFFHHDDHIBGIIEHJJJEGIGFBFB=>9B5??>=F@DFDEB=?B?B@?B@:@@=B PG:Z:SNAP RG:Z:266-1 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1201:4433:4114 73 chrIV 16371 60 100M = 16371 0 CCCCACGGGGGAGCAAAAAACTGCACAAAGTTTGGAGCAAAACTGAGCTCAGGACGGCTTTCCCCAGTTTGAAAAAAAATTTTGTTGCTCTTTTTACCCC @@@FFDFD8CFFHGGGIIJB@HGJFIIJFE.@?ECHGFFD@CEEDECDC;5?CABB@B;5?>@ACC@ACC@:ACCDD9BB@>AAA98:>CC@CCC?>>38 PG:Z:SNAP RG:Z:000 NM:i:3 +BS-DSFCONTROL03:121:D0M8KACXX:5:1201:4433:4114 133 chrIV 16371 0 * = 16371 0 AAAATTAGTTTTTCGTCTTTATCTCAAGTTCTTCTTCATCTTTTTCGATACTTTTTTTTTCTACCCCACGTAAAAAAGTACGCTGAACACGATTTTCAAC :@:4(:4<@9@8>::>:?>>99(@>>>:?@?BBCB?;>5(BCEFIGHGB=67(HED;?BBEIIGDEF@<3<@CDCDDCDCDDDECDDDDDCBBDABDDDCACBD?>@DBA PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1302:19827:42088 133 chrIV 16422 0 * = 16422 0 GCCTTTTGTTTTATCACAACTTTTTTCCATTTGCACTTATGAACTCAATCTTTCTTTCAAAAAATCAGTCTCTCTGAGTAGTATCTTGCACAGAAATTTG CCCFFFFFFFHHGIIIJIJJJJJJJIJJEIIJGIGHGHIJJJJJIJIJJJJJJIGIGIJIEHIIHIIGGHHHHGHEFFBBDBDEEEEEDDDD?CDACCDD PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1216:8211:57172 165 chrIV 16449 0 * = 16449 0 ACATGAAATAATGCGAAATGGATTTTCTAATTTGTAGAAATTATAAGATAGCTCCGAATTCTGCCAAGCAGCCTCTAATAAATGCCGAGTGTACAGACTC ?BBFA=ADHHGHHJJJJJJIJIJJJIHIJIIJJJIIJJJJJJJJIJIJJJJIJJIJJJJJJIJJIJIJEIIHHHHFFFFFFFEEDDDDDBDCDEEECBDC PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:18903:53675 147 chrIV 16449 60 100M = 16174 -375 TTTTTGTTGCTCTTTTTATCCCCAAAAAACCATGTTTTAATTGAATTAAAATTCGGGTTTTGCTCGGTTTTGTTCCAAATTTATGTGCAAGATACTACTC 8DDBBDDCCA:><:@BBB@>;;@>?>FHFFHGHIIHGHBGGGIIGFHGHGGB;GEH@0EGE7IGGHGCEF@BHJIA7?@C==EADFFGFFBE?B?D@=CFFFEFAGGGGFGGGEDEBBBA?BFFFFEDC?DDB?C>C?CA=:=EDDBDGFFGGGGFDGAEEFDF5FFFGDGFB PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:94:17078:10619/1 0 chrIV 16610 60 33M1D38M1D30M * 0 0 ATAAAACAAAAGGCCAAAAAATGACATTTTGCCAAAAAAATTTTTTTTCCGAAAAAGTAGTTTTTCGTCATATCTCAAGTTCTACTTCATCTTTTTAGATA GEGGFGGGDGFFGGGGGGGFGGGFGBGDEDGGGGGGGGGGGGGGGGEEGDGGGFEEFCDECADEBFG@EDAEEBEEC?ABDEDDFEEEEEA?DDBC?BDC@ PG:Z:SNAP RG:Z:266-1 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2211:12400:9160 147 chrIV 16639 60 1M1I18M1D80M = 16457 -282 TTGCCAAAAAAAAATTTTTTCCGAAAAAGTAGTTTTTCGTCTTTATCTCAAGTTCTACTTCATCTTTTTTCATACTTTTTTTGTTTACCCCACGTAAAAA DCDBDDDDDDDDDDDDDDDDDDDDCEDDDDDDDDDDDDEEFFFFFFHHHHHHIIEECIIJJJJJJJIJJHHHFDGJJJJJJJJJIHEHHFFHFFFFFCCB PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2201:10133:75100 165 chrIV 16645 0 * = 16645 0 AATTCACACGGTTCAGGAGTTGGAAATAAAAACGTGGTGTGACATTTTTTCGCAAGCCAAAAAAAACGCGAAAAAACGCGAAAAAGGGGCGGAGTCTGTA #####################################################B@A;/DDFHIGG@GIEIIJIIHFIIJJIJJJJJJHHGHHFFFFFCB@ PG:Z:SNAP RG:Z:000 +BS-DSFCONTROL03:121:D0M8KACXX:5:2201:10133:75100 89 chrIV 16645 60 76M1D24M = 16645 0 AAAAAAATTTTTTTCCGAAAAAGTAGTTTTTCGTCTTTATCTCAAGTTCTACTTCATCTTTTTCGATACTTTTTTTTCTACCCCACGTAAAAAAGTACGC >BCDCA8@@BCB?<50+CDDCC@C@BDDDB@@CDCDCADBCCDDDDEC@;9EEEDFFFHHIIHHEEF=GJJJJJGGGGD?IIGHDIGGHHHHFFDDD@C@ PG:Z:SNAP RG:Z:000 NM:i:5 +EAGLE:2:31:3792:4681/1 0 chrIV 16705 60 101M * 0 0 TTTTGAGATTTTTTTTGTTTACCCTACGTAAAAAAGTACGCTGAACACGATTTTCAACTCAGAATTGAGAAAAGTTCTATGAGTCGGCCGAGCAAGACGAA FBFFF?GGGGDGEGFEGGGEGGGGDGGGGGGFFGGDEGGGDFGFGGFGGGDDFDGGGGGGEEDEGGGGGFGGDEBDFEFFGF?DGEFGGGEGBBEFEEEAE PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:1:48:7607:3697/1 0 chrIV 16768 60 101M * 0 0 ATTGAGAAAAGTTCTATGAGTCGGCCGAGCAAGACGAATAAGTGCCAAATTTTGCCCACTTTCCCGTTTTCGCGAATCTACTTTTTCCATCATAACTCGGT GGGDDGFFGGGGFGGGFDEGEFDFFFF?DFFEFEDFFFFDBDADCFFFFFFG?GGGGFEFGGGGGFBD:BEEEBA@EEBED@?DDDDEDADBD:DB=ABB8 PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:2304:5957:30377 73 chrIV 16778 60 100M = 16778 0 GTTTTATGAGTCGGCCGAGCAAGACGAATAAGTGCCACATTTTGCACACTTTTCTATTTTCGTGAATCTACTTTTTCAATCATAACTCGGTCAGTTTTCA BCCFFFFAHFHHHIFIIIJJJIIIJJIJIJJG>FFHIIJIIIIIIGIJIJGCGIHEHHHHHFFFEAECEACCCDDDDCCDDDD@CCDDBB@B@C:>@CCD PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2304:5957:30377 133 chrIV 16778 0 * = 16778 0 ATGAAGTTTCGTCGCATTAGAAATACTCTGCCAAGAAATATTATTTTTATTAATTTTCAGGCCAAAACTTGTTTTTTTCTACAAATCGACCGGTTTACGA CCA@>DDDDDB?BACCDDDCDDC@@DDDDDDDEEEEFEEDFFFFEHHHHHGHJJJJJJIHFGGJJIGJJJIJJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 +EAGLE:2:84:15670:14903/1 16 chrIV 16791 60 101M * 0 0 GCCGAGCAAGACGAATCAGTGCCGAATTTTGCACACTTCCCCTATTTCGCGAATCTACTTTTTCAATCATAACTCGGTCAGTTTTCAATTTTTCTTAGTTT BCADAEB?CCBDFDCDBEEBGEGEEEEECECFCD@FEDFGGEFFEDDFFFFBGEGGDFFFFFGGGGGFGGGFGFGGGGGGGGGGGGGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:7 +BS-DSFCONTROL03:121:D0M8KACXX:5:1106:14433:6441 165 chrIV 16828 0 * = 16828 0 ACCGTCGGCTGAATTTATAAAAATAGACCATGAGAGGAATTTTTTAAGTCAAACCAAAAAAAACGCGAAAAAACGCGAAAAAGGGGCGGAGTCACCACAC ######################################################################GB:0))*?C9;GE@+C?=385;(@>6.(46;@C=:ACAEHHE;@6FCEDBIGCHD>>CDDDED:CDCDDC?DEEEEEEEEBEEBBCCCEEEEE? PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:43:19134:12725/1 0 chrIV 16956 60 101M * 0 0 AGTGGGAAAATTTTTCAAGAAAAAAATAATTCAAAAATTTAGTACTGGGGGGAGTGGTTTCCTGGGTCTCCTGAACATTTTTTCCACTAAAGTTTTGCGGA FFFFFGEGGGFGGAGGGFGGGGGGGGGGGGGGGGGGDGGGDFEFFGGGGGGGAD@FFCDFGGEFGFEGFGGFGEEEEEGEGGGEGBGGEEBE@DBEBECEE PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:1:57:3806:20180/1 0 chrIV 16957 60 101M * 0 0 GTGGGAAAATTTTTCAAGAAAAAAATAATTCAAAAATTTAGTACTGGGGGGAGTGGTTTCCTGGGTCTCCTGAACATTTTTTCCACTAAAGTTTTGCGGAA CCC:;DD?BDEEDDEEDE=DECECCEDEEEEAE?EEDEE=ACACCBA=ADDA@;DEBCEEEEEEBBE:EEEEEEEBEDEDEEBEEEB?A@C5@@CC?BD?5 PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:28:11193:2776/1 0 chrIV 16966 60 41M1D59M1I * 0 0 TTTTTCAAGAAAAAAATAATTCAAAAATTTAGTACTGGGGGAGTGGTTTCCTGAGTCCCCTGAACATTTTATCCACTAAAGTTTTGCGGAATGTATTTTTA GGGGGGGGEGGDFGGGGGGGGGGGGGGGGGDGGDFGGGGGGGG?GFCFFFGGGEGEGGGGGGEGFEGGGGEEEGFEEEEEGEEFGECEEA?CECCDEEECE PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:63:18448:7383/1 0 chrIV 16991 60 101M * 0 0 AATTTAGTACTGGGGGGAGTGGTTTCCTGGGTCTCCTGAACATTTTATCCACTAAAGTTTTGCGGAATGTATTTTTAATTCATAGTTTTTGATTTTATCTA FDGEGGEGGGGGGFDGGGCGGGEGGGGFGGEAEEEFFDFFFDGGDGGFGGGGGGGGFEGFFFFFFE?FEBBEEEEACEFDFFFAFEGFGGGBFBGDDDGCC PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1213:7683:14705 73 chrIV 16997 60 100M = 16997 0 GTACGGGGTGGAGTAGTTTCCTGGGTCTCCTGAACATTTTTTCCACTAAAGTTTTGCGGAATGTATTTTTGTTTCATAGGTTTTGATTTTATCTAATGGA BBBFDDFF@DHHHDHIHIIJJIJJJCGHJJIHJJIIIJJIJJJIJJJJJJCEHIJIHHFEDDECDDFEEDBACDDDEEDDCCDDDBCDEDEDCDECDDCD PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:1213:7683:14705 133 chrIV 16997 0 * = 16997 0 GTTGAGGGCTATATCTTGTAAACTGGTTTATTTATAGAAACAAACAAGTTTTGGTCTGAAAATTTTGAAAAAATAATATCTCTTGGCAGAGCATTGCTAA CCCFFFFFHHHHHJJJJJJIJJJJJJIIJJJJJJJJIJJJJJJJJJJJGIJJJJJJJJJJJIIJJGHIJJJJHFFCDFFFFEEEEDCDBBDDDDDCDDEC PG:Z:SNAP RG:Z:000 +EAGLE:1:53:14974:4822/1 0 chrIV 17018 60 101M * 0 0 TGGGTTTCCTGAACATTTTTTCCACTAAAGTTTTGCAGAATGTATTTTTGATTCATAGTTTTTGATTTTATCTAATGGAAGATGAAGTTTCGTAACATTAA AABA:=DDDDGF?GGGGGGEEFGGFEDE?E?DDDAE=EBEDEEDEEDEB@?DEDBEBECEEE=EAGDGD:FFCB?FDADEGAFGBFDAEFEEA?E?BAA?A PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:79:7894:12928/1 16 chrIV 17065 60 101M * 0 0 TTGATTCATAGTTTTTGATTTTATCTAATGGAAGATGAAGTTTCGTGTCATTAACCATGCTCTGCCAAGAGATATTATTTTTTTAATTTTCAGGCCAAAAC ECCCDCBAE=DEEEEEEEEEEEDDEE=EEGGEEGGGGEEGDFGGFGFEEAFCGBGGGEGGEGGGDGGEGGGGGGGGGGGGGGGGGFGFFAGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2114:3180:43522 73 chrIV 17244 60 100M = 17244 0 GAGTACAATCCTGTAATAAAATTTTCAAAAATTCGTTTTCCCGCTGACTAATCAGTGATAAACGGTTTTCAAACATATTTTCTCAAATTGTCATTATTGA BCCBDEFFHHHHHBGGGHIJJJIJJJJIGFFAC@EDEEEGEECEF=@C PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2112:12255:61856 99 chrIV 19979 60 100M = 20074 195 TCTTGATACTGCATGAATTTTCTTTCTGCCAAAAATGAAAAAGTTGAGGCATAATCAGCTTTCAATGCTTCCATCGCTTGACGCGGTCCCTTGACTCCAT BBBFDDDFHHHHHJIJIIIJJHIJJJIGIJIJJIJJJJJIJJIGHGJIJJGHHIJIJGBGIGIIJJJJJJEHHJJJHGHEFFFFDDBB;BCCDDDDDDDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2112:12255:61856 147 chrIV 20074 60 100M = 19979 -195 TCCATTCAAGTTGAGATGATTGATAACTTTACACGCGAGTTCTTCAAGTTGTCTTTAAAATCAGAAATGGCCGTACACTTGCAAACGAGAAAACCTACTC AC@DDDDDCDDDDDDDDDDDEDEDCDCCCCAEEGJJJJIHEJIJJJJJJJJJIJJIJJJJGJJIJJJIJJJIIIHHCJJJJJJJJIJHHHHHDFFDBC@B PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2307:13742:62661 99 chrIV 20173 60 100M = 20424 334 TCTTGAGTTGAGCGTAATCATTGCGCTTTAAAACTTGCCCGAAATCCTTAGCGTCTAATAATCGTTTCATAGAATGCGCCACGAAGCACATTACCATTAT ?@@DDBDADDHFBEB';AEFEFEHC?;B566>;;>6>;5'9''3;?5?9CCCCCACCC>:A3 PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:2307:13742:62661 147 chrIV 20424 60 17S83M = 20173 -334 AAAAAATGACGACACCAACACTTTGAATTTAGTACGTACTCTTGCTTTTTGCATTGCAAATTTTTCTTAATTTGAATGTTGAGTTCATCCTTTCTACAAT #################@;)CHC;CC=:ED@EECHDCB2G>F?(0)?9?9E??:IHDH?);HE9BEFBH@IHFA<3CFFA3322++<++:C=E@ PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1207:8708:89857 99 chrIV 22773 60 100M = 23094 421 ATACAGTATATTAGTTTTTAAAAGTTTTGTGCCAAACGAAATTTTGTCGAAAGTTGACAACTTTTTTGTCTAGAATTGGCAATTTTTTAAAAATTTAAAT CCCFFFFFHHHHHJHGIJJJJJJJJJJJJHJJJJJJJJJJJJIJJJIJIIHIIIIIJJJJJJJJJJHHHHFFFFFFB@CEE>ACCDDDDDDDDDDDDEDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1207:8708:89857 147 chrIV 23094 60 100M = 22773 -421 GTTTTTGAATTGAAATTGAAAGAAATCGAGCAACTTGAAGCCGAGGCCGAAAACACAATTCCAAAAATACCCAAGAAAGCAGCTGGCTCATCTTCAGAGC @DDDDCCEEDDCDEEDCDDDDDBCA PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:71:9405:4095/1 0 chrV 3773 60 101M * 0 0 TGATACACGATCTTGAGAAGAGCTCTGTCCTCCAGGCTGCGAACTGGAGCGTAGAAAGTTTTCATATTGTTAAAAACTGCAAAGAAATGTTCGATTCCTTG DA=DADDBB:;?@@@=DDDDDDD?DDDBDDCDDDDDDDDDDB?C=DDADDD=DDB?AA:ADCDDCCBCDB=:@ACDBCA=ABCBDB@>A@ABC??BB=BBB PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:65:16164:2544/1 16 chrV 3846 60 101M * 0 0 AAACTGCAAAGAAATGTTCGATTCCTTGTTCTGACGTGTGATGCTTTCTCAAATATGGAATTAAATGACAGACAAGGAGATGAAGTTTCGGAGTGACTCAT @?@C@B;>EDDBDDBEEEABBA@B=C:EFEFFDBFEEEEEEADDCBEDBDBFD5FFFFFBFFFEBEFEAFFFAFFFFFFFFEEDEE?D=GGGGFGGGFGFG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1214:8214:10158 99 chrV 3997 60 100M = 4220 323 GTTAAAGCAAAACTTCAATTTTGTCAATCTCTTGGTCTAAATACGTTCTATCGTTAGATAAATCTAACTATCTAACTGAAACGAGTACAACTATATTGTA C@@FFFFFHGHHHJJJJIJJJJJIIJIJJJJJJJJFHIJJJJJIJGIJJJIJJJJJJJJJJJJJJIJIIIJIIIIDGDHHEHGFFB>CCCEEDDDDEEAC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2201:16390:41463 147 chrV 3998 60 100M = 3761 -337 TTAAAGCAAAACTTCAATTTTGTCAATCTCTTGGTCTCAATACGTTCTATCGTTAGATAAATCTAACTATCTAACTGAAACGAGTACAACTATATTGTAA DDCCC>DC@ECEEC@EEFFEFHHE>HGIIIGHCEC=/)FIHFHIJIIGIHIJJJIHIIIJJIIIIGGJIJJJJJJIIJIHIJJIIGGHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1214:8214:10158 147 chrV 4220 60 100M = 3997 -323 AAAATTAGTTTAATCTCCTCGCAAGATTTTTTTCTCCGAAATTTAAGATTGGGCGCTTAGTGATATCATAACTCTGCAGCCATCAGAAAAACTGAAATGT CDEDDDDCCDCCA@BBDD@BC>CCDB@CEFHHFIJIJHIIIGJIJIIIIHJJIJGHIIJJIGJJIJIHGHHGFJJJJJIJJIEJJJJHGHHGFDFFF@@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:76:4095:13262/1 16 chrV 4714 60 101M * 0 0 ATCGTAAGGCGGTACTTTTTAAAATTTCGTTTCCATTCTTCCCCAGGGATCCCGTAAAAAACCAATTTTTCGACTAGGTCTCACATTTTGCCCTACAAGAG B=BC:CACBCACA=ECD:CDGA?AA:ADA:A@EEEBCGDAGGGGEGGGEDGGGGFFGGFGBFFFFFFGGEGGGGEGGGGGFGDGGGGFGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:89:14779:18797/1 0 chrV 4998 60 101M * 0 0 TGTGTTTGGTTTCATTCAGACGCTACTTGCCTGATCTATCAGTCTAACAATTTTTGTTTAATTTTCTTCACTATCCGCCGTTTATAGTTCGTTCTTCCGTA EEE=FEFEEEFFGGGFGGFGGGGDGGGGGGGFD?DFFEFCFGDGGEGGEEGGGGGGEGG=FGGFFFGEEGGFEGGEGGGGEGG@EDDADDEEEBGDDEDCE PG:Z:SNAP RG:Z:266-1 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1302:16740:19201 99 chrV 5929 60 100M = 6103 274 CTCCAGGAAGACGAAAATTCCACAGACTGAGTATCCACGTAAGTATACGATTTTTCTATGCGCTATAAAATTCTTTAAAATCCGCTATTTTCCAGCTGAT CCCFFFFFGHGHGJJJJJJJJJJJIIJIIGGCGIIJIIJHIJJDHIIJJIIIJJJJJJJJJJJHGFFFFFFFCEEEEEEDEDDDDD=BDEEEDEDDDDCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2115:16987:87976 99 chrV 5959 60 82M18S = 6130 228 GAATCCACGTAAGTATACGATTTTTCTATGCGCTATAAAATTCTTTAAAATCCGCTATTTTCCAGCTGATTACGCATCTAACGACCTGATGAACAGTTTC 1++=+2+22+2A:HD?E,A+++32+++2A@*:8C):BBGD4*9B9*?*099/?6(6@'.@E;)).==)7;7?),5;ACA53@################## PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:2:85:10419:2317/1 0 chrV 5963 60 101M * 0 0 CCACGTAAGTATACGATTTTTCTATGCGCTATAAAATTCTTTAAAATCCGCTATTTTCCAGCTGATTACGCATCTAACGACCTGATGAACAGTTTCAATGG GGGGGGGGGEFFFFFBDGGGGGGGGGGGGGGGGGEGGDGGGGGFEFGFEFEEBEGFDGEEGEGGBEGEBGGDEEEEBEEE?DCDCEE?EECE;CCACCCC? PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1302:16740:19201 147 chrV 6103 60 100M = 5929 -274 CACCTATGCGTCACGCTATGGTCCCGCATATCTTCGCGAACGATTCGGAAGCCATGATTCCCAACTTGAGCGTATGCTTTATGGCGGCAGTGGACCACAG @:CCDDBBDDDB5A:?BADADA???5@DA:EEBEFBFBBBACAEA?EEBCBBBABEEFGBGGGFGGGFFEFFDBGGGGFDGFGFGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:47:2986:6932/1 16 chrV 10038 60 101M * 0 0 GAAGATCTAATAGCTTTGTAAAATTGATAATTTATCGTCAAAAATTTGACTTGAAACAGTCTAAATCGTGCAAAATACATACTTTTCTATTGTTTTATCAA EEEEEEEEEEEBBFEBGEEEGEFF?EEGEEGGGGEGGGEGFEEE?EFFCFFFF=FCGFGGGGFGG?GGGGGECCEDEGGFDGDGFFGDGEFGGGGGFFGGF PG:Z:SNAP RG:Z:266-1 NM:i:2 +EAGLE:2:104:13973:5102/1 0 chrV 10071 60 101M * 0 0 ATTATCAAAAATTTGACTTGAAACAGTCTAAATCGTGAAAAATACATACTTTTCTATTGTTTTATCAATTATTTGAACACTTTAAAATTTAATTTAGCTAA FGGGGGGGGGGGEGGGGGEGGFGGG=EFFFEGGFGGGGBGGGGAGGFFGGGGFGGGGGGEGGGGFGFGGGGGGG?DDGEGGBGFGGFFGAGFGGGGFFGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:113:10088:6922/1 0 chrV 10199 60 101M * 0 0 CAAAATGCTGCTGGTCTGGCAAGAATAGGGCTGCAAGACTATTACCGTATTTCCTCTATTAGTCTTGCATGCAATACTAACAGAGGCTGCAAGACTAATTT GGGGFGGGGGGGGGEGGGGGGFFGGGFGGFGGGFDFGEGEGDGGGFFDBFGEGGEE=E?EFGBGBEEEEDFCEEEEEEBDGEE?EEE@ED:@B59ADDACD PG:Z:SNAP RG:Z:266-1 NM:i:7 +BS-DSFCONTROL03:121:D0M8KACXX:5:2313:7454:29242 99 chrV 11315 60 100M = 11637 422 GTCTACGGGAGGGGGATCCTCTTGTGTAATACGTTATTAAATTATGCCTGTCAGAGCAGCAACACACACAGACAAGTGAGTCGTCGACTCAAAAAAAGAA CCCFFFFFHHFHHJJGIHIGIJIICHGGIIJJGEHIIJGGIDHIJGIIGIHHHCEHFFFFFEEEDDDDDDBDBDCACDCDACBBDDDDDBDDDD8>>5?? PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2313:7454:29242 147 chrV 11637 60 100M = 11315 -422 ATGCGGGAGGAAATCGTAGACATCTCAACTGATTTCGCATGGTTAAGAGTGTGCTGACGTCACAATTTTTCTGGGAAAAAATTCCGCATTTTTGTAGATC @DD@BCDDEDDB?=BDEDDDDDDDDCDEDDDDDDFFDDGGHHHHJJJIGHIJJJJIIGGJJGIJJJJIIJJJJIJJJJJJJHJJJJJHHHHHFFFFF@@C PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:5368:22825 73 chrV 11689 60 36M1I5M1I17M40S = 11689 0 GGTGACGTTACAATTTTTCTGGGAAAAAATTCCCCAATTTTTTGCAGATGAAACCGCAATGAGACTTTCTGATAAAACGTCAGTACTCCACACGGCCGAA ++:+AD7D<+A,++AFE:F:,+++2++<:DB4:?)))0*000?'=8=/CFC;@@6@:A6B######################################## PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:5368:22825 133 chrV 11689 0 * = 11689 0 TTTCTCAAAAATGTCACGTCAGCACGTTCTTCATAATGAAAAATCAATTGAGAATCCCGCATTTGTTTGTAGATCTCCGTAGATCAAACCGAAATCGGGC ################################@@DAA>D?@??;)==.)==.7E@8FD@FFFB>BBB::0?01))1)?CF<94BE?E<CDDDDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:14381:50677 147 chrV 12708 60 100M = 12587 -221 TTAAAAAAAACAATGTGACTTGACATCACCGTCTGTGTTCGTCTAATGAAAAACAGCAGACCGAAAAAAAAACGGGTCTGTTTTAATTAAAAACTGATAG :1DB@DCC>@>AADCCA@CC>:>>>22&902?A:E@;;D@FEEHE>@GEDF;;JJIIGGGGEIJJJIJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:100:2124:19174/1 0 chrV 13308 60 101M * 0 0 TTTGATTTTCCTCTAGAATGTTGCTTCCGTCTCCTCAATCCATTTTCATTCCACATAGATTCTAAAACAGAAAAAAATCGATTCAGAAAAAAGTTAACAAA ?EEDEAFFFFFFGEGGAGDGGGGFGGGGGDEGGGDGGGDDE5EDEEFEAFGGGDDB?GGFGFGDDGGFGA?GGBFCDAEAGEEFBAED?EA>A=ADDAEEG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2305:14519:87038 73 chrV 14262 60 100M = 14262 0 ATATTAGGTCTCCAAATAAGTTCCGGGTCAAAAATCATAACTTTGTTCGCTGCGTATCGATTTTTATGAAATTGTGGGAATTTATGTTATCAACCATGAT CCCFFFFFHHHHHJJJJJJJJIJJJJJHIJJJJJJJJJJJJJJJJJJJJJJJJJHIJJJEHHHHFFFFEDEEEEEABDBDDDDDFDFEEECDDDDDDDDC PG:Z:SNAP RG:Z:000 NM:i:8 +BS-DSFCONTROL03:121:D0M8KACXX:5:2305:14519:87038 133 chrV 14262 0 * = 14262 0 TTCATTTGACAATACTCACAAAATTTTTTTGCCGTCCGAAGTGCCCTAACTCGGAGCCAAATTTTTCAGGCATTTTTCAGATCTCGCTTCTTTTACGCTT DDDDDDEEEDEDC@DDDDDDDDDDDDDDDDDDDDFFFHHHHJJJJJIIGFIJJJIJJJJJJIJJJJJJJJJJJJJJJJJJJIIHJJJHHHHHFDDDFCC@ PG:Z:SNAP RG:Z:000 +EAGLE:1:73:5284:8999/1 0 chrV 14265 60 101M * 0 0 TTAGGTCTCCAAATAAGTTCCGGGTCAAAAATCATAACTTTGTTCGCTGCGTATCGATTTTTATGAAACTTTGGGAATTTATGTTATCAACCATGATCTTT GGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGGGGGGGGGGGEGCGGGGGGGGGGFFDGGFGGGDGEDBGGGGGEGGGGCGGGGGGEBFFGF PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:83:17647:3047/1 0 chrV 14268 60 101M * 0 0 GGTCTCCAAATAAGTTCCGGGTCAAAAATCATAACTTTGTTCGCTGCGTATCGATTTTTATGAAACTTTGGGAATTTATGTTATCAACCATGATCTTTCAT =E?DEDE?EDBDDD=DBDBDAAAACDFFFFFDEFF?CDD@DE=?BAA??A=EEEE?FFFF@EFFDBFBEFFEDF=BE?BE=@C?CBE@EEEEBE@EEADDD PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:24:16337:8220/1 16 chrV 14278 60 101M * 0 0 TAAGTTCCGGGTCAAAAATCATAACTTTGTTCGCTGCGTATCGATTTTTATGAAACTGTGGGAATTTATGTTATCAACCATGATCTTTCATTTGACAATAG =DD@ECEBCEC?DFGGFFEFFEE5DEFEDDDGEGGGDGBGGGFFGGEGFGFGGGEGAGGGGEEEE?DGGGGFGGFGGGGGGGGDGGGAGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:2:7:10444:9975/1 16 chrV 14287 60 101M * 0 0 GGTCAAAAATCATAACTTTGTTCGCTGCTTATCGATTTTTATGAAACTGTATGAATTTACGTTATCAATCATGATCTTTCATTTGACAATAGTCACAAAAT BBEEDEEBEEDBEE:E=EEEEAD:BD?EEAEEEBABEEDBDDDEA?EEDEADBDBDDEBEBEE?EEE==EECA=;=DDB?AEBC=EDDD=DE:EBEDE=AE PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:59:15165:16923/1 0 chrV 14304 60 88M1I12M * 0 0 TTGTTCGCTGCGTATCGATTTTTATGAAACTTTGGGAATTTATGTTATCAACTATGATCTTTCATTTGACAATAGTCACAACATTTTTTGACCACCCCAAG EGFGGEGDEDFFFBFGGAGGFGGGG@FFEFGGGAGGEFDGGBGGGG=GEFGFGCGAGFEGGFBGGCEEEE?C@BBAFEEFFFEBFFGDB?=FEDEGEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:42:11999:2677/1 0 chrV 14317 60 75M1I25M * 0 0 ATCGATTTTTATGAAACTTTGGGAATTTATGTTATCAACCATGATCTTTCAATTGACAATAGTCACAACATTTTTTGACCACCCCAAGTGTCCTAACTCGG GBGFDGGGDFAECFEFFGGFFEFDFEFF?EGAFGGGGGGG=EBBBEFFFFABCC=EDFEDEE@EBEFEEDEEEDGEB:?EEEEB=8BB:E5AA@:@@AAAD PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:2:11:9361:9333/1 16 chrV 14441 60 53M1D48M * 0 0 CACGGCAAATTCGGCAAATTTGCCGTGCTTAACAAACTCCAAAAAATGTGACATTTTTTGATGTTTTTTGGAGCACCAAAACTACTGAGTTCTGAACAAAA =CEEDBGFGEBGEEFGEGDEEGGGGDGGGDFEGGGEGFGGGGGGGGGGGGGGGGGE=FGGGGGGFGDGGGGEGAEFGGGGGAGGGGGGGGGGGGFGGGG?F PG:Z:SNAP RG:Z:266-1 NM:i:7 +EAGLE:1:82:11123:16509/1 16 chrV 14444 60 53M1D13M1I34M * 0 0 GGCAAATTCGGCAAATTTACCGTGCTTAACAAACTCCGAAAAATTTGATACTTTTTGATGTTTTTTTGGAGCACCAAAACTACTAAATTCTGAACAAAAAG BCBCACB=EEEAFGFEDFDFGGFFGFFF?FFEBEEADGGDGGGGGFGGGEGBDDGGGF@GDC@C@@5EFFFGFEEGDFFFFEFDFF=EEEBCCCEDGFGDD PG:Z:SNAP RG:Z:266-1 NM:i:8 +EAGLE:2:120:10149:2029/1 16 chrV 17145 60 101M * 0 0 TTTTTTTTGTTGTTTTTTTGTTTTGCGAAAAACTTTTGAAAGTGTGATCCCACGTTCTTTTCTTGTCTGCGTCTCTTTTAAATTTGGGTTCTCTCGATAAA ?DDCDDADEE?CCED:DD5DDEF?FE?DEAEFFF:F??:DDFDFDFFF==DCBEEEB?EDEFD=DEFBFDFFFF?FFAFDFD:EB5C PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:42:18060:2997/1 16 chrV 23388 60 101M * 0 0 TTTTTCCGATTTAATTTTTGATAGGTTTTCTCCGCAAATTTTCCACTTCAGTGCATGGCACACAAACGGCATGTTATAGTAATTTTCCAATTTTCCAAAAG FDDECFADDB@FEEGED?EGGGFBEGFG?GEGGFGGFFGFGGGDCGGCGGEFGGDGGGFEGDFFFDGGGGEGGGFFGGGGDGGGGGGGGFGGEGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:13:6904:13626/1 0 chrV 24494 60 101M * 0 0 GGTTTTTCTTGAAATATCGTTTTTAAGGTCAATTGATATGATAATAAAATATATTGTTGACCTTAAAACACATTAAAAAAACTTCGACAAGAACCGACAAA EDEDABDA8BCF=FF=EEEDFFDFFFDA=FFEDEFBFADF:EDEED=FEEEFFD?DAEEB=DEDEFEDFCEABEEFFE?B8CDBFAFDFFFEFEBFEFEDE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:47:11060:17697/1 0 chrV 24606 60 101M * 0 0 CGGTTGAAAACGATAAAGATAAAACATTTCGTAAATATACACAAATCTCGTAAATCGACACATGGCGTTTTTGGCGCGAAAATTCGGCGTTTGAAAAACTT GGGEGGGFAGGGGADGFDDGGGGGGGGGFGGEGEGDGGGGGGDFDGGGFEDGGGFGEFGGGGBF=EE@BCFEFFDFEFEEEEEEGEBAFB=E==BFEDE@B PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2112:2411:63807 99 chrV 24799 60 100M = 24916 217 AAGAGTACCTAACCAAAGAGCGACTCATCAATTTTCTGAATGAAGAACAACGTGATCCACGACTCAACGAGATCCTCTTCCCATTTTTTGATTCTCAGCG @BCFFDDFFHGHHJJJJJJIJJIIGIGGGHGIJJJJJIIJJJIJJJJJIJJICGGGHGIEHGFHFFFFFDC>BDDCDC@CCCCCDEDDDDBCDDDDDD<@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2112:2411:63807 147 chrV 24916 60 100M = 24799 -217 AAAAACACGAAAACGACATAAAATATCAAGAAGATGGAAAAATGTCCGGTGACGGTTTTCTCCGATTCCTGATGTCAGACGAGAACCCGCCAGTGTTTCT ?CC?8<<-:BB4DC@::A@@CCAC>:44>(DDDC@5=;??@;;;;8?;)B6/:55()@=)@8)==.))6DB6?990B?JJHHGHHFFFFF@C@ PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:2:45:11954:18961/1 0 chrX 1563 60 76M2I23M * 0 0 TCACAAAGTTGCAGCTCCGAAAAGAGAATCCGTCTGGAATTCTGGAAGTTCACAAGAATTGTGAGTACTTACCTTTTTCAAGTGAAACTAGAGGAAAAATT GGGGGGGGGGGFGGGGGGGGGGGGFGGGGGG=GGFGGFFGGGGFGEDFBDGFGGGGAGGGFCFEFCDDEEGGGFGFGGEAFFBFCEEFEDDCDGAADEBDB PG:Z:SNAP RG:Z:266-1 NM:i:6 +BS-DSFCONTROL03:121:D0M8KACXX:5:1311:7062:60667 99 chrX 5712 60 100M = 5891 279 AATAAAGTCCTGCCGCGAATGCTTCAAGGTTACTGTAGTTTTTGGTGGTGAGACCCGGAAATTTCTTTATTTTTCAAAAATTTCAGAACCCGGTCATGAT CCCFFFFFHHHHHJJJJJJJJJJJJJJJJGIJJJJHIJIIJJJJIHIJAGIJIHHHFFBDDDDDDEDDDDDEEDDCDDDDDDDDECDDDDDDDBBDDDDC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1311:7062:60667 147 chrX 5891 60 100M = 5712 -279 TCTTGGTGGGTCCCGTCACGAATTTTTGGAAATTATAGTAAAACGCCAATTTCATACTGATTTTGATGAATTTTAACAATAAATTAGCAGTATTTTAAGA DBBDDDDDDDB>DDFFFFHHHHJJIGGJJJJJIJJIJIJJJIHHJJIJJIJJJJIIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1306:15859:17118 99 chrX 5939 60 100M = 6055 216 ATTTCATACTGATTTTGATGAATTTTAACAATAAATTAGCAGTATTTTAAGATTCTGAAATTAATTTTTTACTGAATTTGGCGAAAAATTGCAAATTTTT CCCFFFFFGHHHHJJJIIGGIGIIJJCHHJII@HHIIJIGJJHIIJGICB?GGHIIJGHHJBGEIFIHHJIJJJIJGIJ;@CHD<>@@A PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1306:15859:17118 147 chrX 6055 60 1M1I3M1D95M = 5939 -216 AACCTGGTGGTTTTCGTGGCGGGACCCGGAAATTTCTGAATTTACGCAAACCTGGTCATGATTGTACTTAAATGAAAGCTCCTAGCGAACTGAAATCGAA @CBA@BDDBDDBBB=BDBBBAAADDDDHHGHEGIGIJIIIJIGGGEHHF?IHJIIIJJJJIJJIIIHHDIIIHIIIGJIIJJJJIIHFFHGHFFDFDCCC PG:Z:SNAP RG:Z:000 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:6622:23752 99 chrX 6879 60 100M = 7127 348 TTTTCAAATTTTTTTGGAAGTTTTCCAAAAAATCTCTGTTTTTCCCGCCGCTCAAAACCCTCAATTTTTCAGATTTCCCTGCTGGAAATGCTCGATTGCA CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJIJJJJJIJJJJJIJIJJJJEFFDEEEEDDDDDDDEDDDDDDCDDDDEDDDDDDDDACCACCCBBDDDA: PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:77:8575:6989/1 0 chrX 6976 60 101M * 0 0 GCATTGAAATTATCACTGAAGAATTCAAATCTCCAAAAATGACGTCTCCGTTCTCATCGCCATCTACCCGACAATCAATCACTTATATTCAAGCGAAAATC GGGGGGFFGFGGGGGGGGGGGGGGGGGFCGGGGGGFBGFGGFGGEGEFGFEGGEGGGGEGEEEGEEEGGECD@CCFCD@FAECC@CAACECDBCDA?CCCB PG:Z:SNAP RG:Z:266-1 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:6622:23752 147 chrX 7127 60 100M = 6879 -348 CACAATGTCATCGAAACCACTCTCTTAAAACTGCAAATTTCTGGATTTTCGACCTTAAAATTTTTAAAAATTCCCAAAAAATCAAATTTCTGTCAAAATT CEDDDDDDDDDDDDDCDCCDEEEEDEFFFDFFFHHHHHHJJJJJJIJIIHGIJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:118:7183:17666/1 16 chrX 7624 60 101M * 0 0 AAACTTTCTTGGGTTATGAGTCTCAACGTATCCACGTGCATGACGGAAGAATTGGCTCAAATGCTCGCGAAGCTGCTCGGCAGACGTGTAAACGTCCAAAA 9=:?7@?C@CACA?ECCC=CCC:B?5B@BA:A=CCEACDDBEEFFGFBBGGEGGDDGGGGF=EGFGFFGGGGGGEGGBEGGGGGGGGGGDGGGGFGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:2:55:11225:9062/1 16 chrX 7903 60 101M * 0 0 CAAAAAATGAAAATTTGGGCATAGCTTCCGCATCTCCAGTGACAAATGTGCTCTATTGCACAAATTGTTGGTTGGAATACGGTATTTATTGCATGTTTCAG EGEEEEEEEEEADBBDGGFEEEDBEBCEFDGEEGEGGGFAFEEGGGFGGGEGFGGEFGGEGFEEFGFGGGGAGGGGGFGGGGFGGGEGGFGGGDGFDGFGG PG:Z:SNAP RG:Z:266-1 NM:i:3 +EAGLE:2:78:6338:2856/1 0 chrX 7939 60 101M * 0 0 CGGTGACAAATGTGCTCTATTGCACAAATTGTTGGTTGGAATACGGTATTTATTGCATATTTCAGCCGAGAATACCACTATTCAAACCATCAATTTGGATA DDD@DEEEEEGBDEGFGGFGGGGEGGFGEGGEBFEBEFFADDEFFFEEEEEDEEEEEDEEEEGBEEEEEEEEEEEEEEAEEEEEEEEE@EEEEAECCCABE PG:Z:SNAP RG:Z:266-1 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:1115:15278:33162 73 chrX 7942 60 100M = 7942 0 TGACAAGTGTGCTCTATTGCACAAATTGTTGGTTGGAATACGGTATTTATTGCATATTTCAGCCGAGAATACCACTATTCAAACCATCAATTCGGATAGA @@@FDDDDFDDDAGFHJJGIDHICFHEFCBFGGBDA@BDDGEH:?GA>GEFGDCDEEEFDEE??>CA@A@C@B@DBA> PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1115:15278:33162 133 chrX 7942 0 * = 7942 0 TAATAAGCCACTTTTCAAATAATCGCTGAATTCTAAGTAAAACATGTTTATTTTCAGTATATTTATCAGATTTAAAAAAAAAAGTGAGATTGTGTTAGTT ##########A:3CC;AB;)7)))3=.7.))=D=.))));=;=)8BCB:@BAACEEDEF PG:Z:SNAP RG:Z:266-1 NM:i:6 +EAGLE:1:75:12666:4476/1 0 chrX 10543 60 87M1D12M1I1M * 0 0 TTGGGCATAGCTTCCGCATGTCCGAGTGACAAATGTGCTCTATTGTCAAATTGTTGGCAGAAATACGGTATTTATTCACATTTTCAGCCAATTTAAGGCTG GGGFDGFFFGGGGGGGGGGFDGGGGGBGGGFFFFFFFDGGGGGGFGGFGFGEDGGAEGGGDGGGGGFFEEFFFEDGGEGGGGGGGDFGGEBDFG=EEFFCB PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:2:56:15981:20326/1 0 chrX 10549 60 81M1D20M * 0 0 ATAGGTTCCGCATCTCCGAGTGACAAATGTGCTCTATTGTCAAATTGTTGGCAGAAATACGGTATTTATTCACATTTTCAGCCAATTTAAGGCTGAAAATG FFFFFEGGGEFGGGGGGGCEBGGGGGDFEDGFFEGFGFGEEEEEGGGEEEBFAEADDBDBEE>EEEEBEE?:???ABA??AAA@AABB?B88A>?BBBBC:C;C?@;:C?:@CCAC?CA>AA=5BB;:5A?:D=?DDDD5?D PG:Z:SNAP RG:Z:266-1 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:2213:8832:90154 99 chrX 11838 60 100M = 12309 571 GTCTCCAGCTTCAAAATGACCCCCATCATGCCCTGTTTCCGTGCAATTTGAAAAATTCGGGTCTCGCCACGCGTTTTTTTTTGCAATTTTCGAACGAACT CCCFFFFFHHGHHJJIJJJJJJJJJJJIIIIJJIJFHIJJIIIIHIIIJJBGGGJJJJFIIEHGEFHFFFDDDD6;@BDBDDDDECDDDDCBBBDDDDBA PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2213:8832:90154 147 chrX 12309 60 100M = 11838 -571 TCAAAAATATTGAAAAGCCACCGATAGAGAGGATTTAGTGTGAAGAAAAAGGGGTGAAAAAACACCGGAATTGGGAGAGATATAGGACACGTCCATATCT 05CDEECD@C:5C>??@BD?@DCCEEEEDDFFCDFHHHHHED@7)JJJIIJJIJIJJIHGIGHGIHGJIJIJJJIGIIIJIGIJIIJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1101:20052:62684 99 chrX 12814 60 94M6S = 12857 139 CTGTATAACAGTGTGTAATTTCAAAAAAATCGGACGCACACACGCTGACTTGTGAGGCTTCAAAAAAAAAGTTGAAATAGTCAAAAAAAAAATCCATCAT CCCFFFFFHHHFHHHIHIJJJJJJJJJJJHGHJIJJJJJJJJIHIHHHFEFFFFDEECDDDDDDDDDDDB>:CCC@CACC:CCDDDDDDDDD>:8-BDDDDDDDDCCA>DDC:C><=DDFHHGFG@IGGJJJJJJJGHEIGIIHBIJJJJIIIGJJIHGIIHHHFHFFFFDB@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:61:3791:13082/1 0 chrX 13027 60 101M * 0 0 AAAATATGAAAATGTGATAGAGTGAATCATTGCGATTCCAATGAGGTAAAAAACTTGGCAAAATTCGACAAAAATTTCATTTTGACACTAAACACTTTATT GGGGGEFE?GEEDBEEEEEEFGFGGEFFEADGGGGE?GFFGGFGGGDGFGFFGFFFFBGGBFEEFGGGFGGDGEEBGEEEFFGDBEBGFEGFEEEGEEBGD PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2313:12490:88378 99 chrX 13038 60 100M = 13177 239 ATGTGATAGAGTGAATCATTGCGATTCCAATGAGGTAAAAAACTTGGCAAAATTCGACAAAAATTTCATTTTGACACTAAACACTTTATTCAAAACACTA CCCDFFFFHHHHHJJJJIJJJIJJJJJJJJJJJJJHIJJJJJJJJJJJJJJJJJI7FHJJJIJJHHHHHHHFFFFFFEEEEEDDDDDDDDEEEDDDDDDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2313:12490:88378 147 chrX 13177 60 100M = 13038 -239 TATTTCTCAAAAAAGATTATCTTAAAATGTTGAAAATGATTATCTTAAAGATTATCTTAAAATGTTGAAAAAAAAAACACCGACTACCGTAACCCAGAAC @DCCBB?BDDDDDDDDDDDCDCDDDDCDDDDEEFDCCCDDDDDEEDEEECEFFFEFFHHHHEAGIGGJJJJJJJJGGGFJIIIIHGJHHHHFFFFFFBBB PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:84:6328:7727/1 0 chrX 15454 60 49M1I37M2I12M * 0 0 CAGTAACCTGACGCTGAATTTCGCGGCGAGACCCAAAAATTCCGAAAAAACCATCAAAAACAATCAAAATCTGCCAAGAAGGAAGGGGGTTTGATTTAAGT GFGFGGGGGGEGGGGGGFDFGGGGBGGFFBEGEGBGGGFBBGFEDGFGBG?GGEFFGGGFGEE7EBDEFDDDECFEECEE@EACCCCCC6:AA@:AC?C@; PG:Z:SNAP RG:Z:266-1 NM:i:5 +BS-DSFCONTROL03:121:D0M8KACXX:5:1205:12522:62956 99 chrX 21169 60 100M = 21409 340 GTATAATCATGACTAGGTTCCGGTAATTTCGGAAAATATTGGGTCCCGAAGCGAAATTCCCCGGGTTACTGTAGGCCTTCGCGGCGAGACCCAAAAATTT @?@FFFFFHHHGHJIJJCFIIJFIJIGIJIIGDGGIFIIJGIJDFHIIBG>HEDGHFHFFFFDDBBBDDDCCC>C>>CAC@BB9999>09@ABBDDA<:> PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1205:12522:62956 147 chrX 21409 60 100M = 21169 -340 ACCTAAAATTTCGCGAGAAATTCAATTTTCAAGCGAAAAATTTTAATGTCCGAACGCAGATCGTCGAGAGCTTTCATTTAAGTATAACCATGCCTTAGTT CCC@DC@B>@@BB?>CCCADDDDDDBEEFCEAEECDJIIGHGGIHF?GEGJIIEIHDAACC PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:24:11474:6931/1 16 MtDNA 422 60 101M * 0 0 GGTTTAAGAGGTTTTTATTATAGTATTTACTGGTTTATTTTTTGTTTTATTTTAGTATGTTTATTATTTTTTATAAATTTTAGTAGTTATTTTTTACATTT DCCCA5:DDBC>BCD5D:DD=:D6DDDDBDD?CCCCACCCCCDCB=DDDBDDCDDDDBCCCC6>>;@#### PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2306:21063:70183 147 MtDNA 1595 60 9S91M = 1578 -108 CTAGTAATAAAACTATTTTTAAATATGTTTTGAAAACATGTTTTGAGGTAACTCGTAGTTTTTAAGAGTTAGGTTAATATAGAATTGTTGACTGTTAATC #########@B>67;3@;B?JIIIGFC9A2?C<++JJJJIIEIHFC;EJHHHHHFFEDDBB@ PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:2:62:8987:8931/1 0 MtDNA 1620 60 101M * 0 0 AACATGTTTTGAGGTAACTCGTAGTTTTTAAGAGTTAGTTTAATATAGAATTGTTGACTGTTAATCAAAAGGTGTACCTCTTAATATAAGAGTTTAGTTTA GGGGGGGGGGGGGGFGGGGGGGGGEFGGGGEGDGEFFFDFGGGGGGGGGGGGGEGFEGGGEFDFGGFFFEFFAFECEEEFEGEEGEGEEGBFBEEEDCDEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:58:15307:9170/1 0 MtDNA 1657 60 101M * 0 0 GTTTAATATAGAATTGTTGACTGTTAATCAAAAGGTGTACCTCTTAATATAAGAGTTTAGTTTAAGTTAAAACGTTAGATTGTAAATCTAAAGATTATTGC DBDD:CDCBCEBAEEEC=EEEBCBBB=BBB@>>CC5C>AA?>>;>@@FBD@@E:B@@@BB@@5B?B>BBBAAA:A@BB@@@D@@@@@A?BA?@>@@@@@@? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:87:9699:18394/1 16 MtDNA 1707 60 101M * 0 0 AAGAGTTTAGTTTAAGTTAAAACGTTAGATTGTAAATCTAAAGATTATTGCTCTTGATAATTTTAGTTTTACTTATAGTTATTTTAATGATGATTTTTATT BEFGEBEEGEEEEEEGBEGGEEFGEGGGGGGGGGDGFEGEGGGGGFDGGGGGGGDGGBGGBGGGGGFGGGEGGGEGGGGGGGGFGGGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:100:3634:12538/1 16 MtDNA 1809 60 101M * 0 0 TTCAAAGAATCGCTTTTATTACTCTATATGAGGGTCATTTATTGGGAAGAAGACAAAATCGTCTAGGGCCCACCAAGGTTACATTTATGGGATTAGCACAA EA=EE??ED:EAB>8;;9?EE?EEEEEDDDEAEEEBEDEEEEEEEEEEEEEEEEEEEEEEEEEEEEEA?FGGGGDDGEGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1111:21372:42529 99 MtDNA 1832 60 100M = 1878 -100 CTATATGAGCGTCATTTATTGGGAAGAAGACAAAATCGTCTAGGGCCCACCAAGGTTACATTTATGGGATTAGCACAAGCTTTATTGGATGGGGTTAAAC @B@FFFFFHHHHHJIIJFHIIJJHGIIHHGJJJJJIIHGHIIJJJBFHJIFGGFHEIHIIIHHACHHFEFFFEEEECEBBCDDCDDDCCDDD@DBB?CAC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1111:21372:42529 147 MtDNA 1878 60 46S53M1S = 1832 -100 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCACCAANGTTACATTTATGGGATTAGCACAAGCTTTATTGGATGGGGTTAAAN ##############################################IHGFF?0#IIGEIIJIIJJJJJIIIJJJIIJJJIHHHHIJJHHGHHFDFDD=1# PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1109:13191:17203 99 MtDNA 1898 60 100M = 1981 183 GGATTAGCACAAGCTTTATTGGATGGGGTTAAACTTTTAAAAAAAGAACAAATAACACCCTTAAATTCCTCTGAAGTATCATTTTTACTTGTACCAGGAA BCCFFFFFHHHHHJJJJIJJJJJJJJJJFHIJJJIIIJIIIJJIJJJJJJGIIJJJJJHHHFFFFFFEEEEEDEDDCDFEEEEEEDBDDDDDCEDDDDD< PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:73:15682:20928/1 0 MtDNA 1914 60 101M * 0 0 TATTGGATGGGGTTAAACTTTTAAAAAAAGAACAAATAACACCCTTAAATTCCTCTGAAGTATCATTTTTACTTGTACCAGGAATTTCTTTTGTTGTAATA GGGGGGGGGGGGEGGGGDGGGGFGGGEGEGEGGEGGFGFGGGGGBE?GFFEEGEGEECEC=CCCCEEEEECC@D?ACEC:CDACCEEEEEEE;EE6;ACECCCACBCBDDDDDFDDEDDDA PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:64:1358:10984/1 0 MtDNA 1931 60 101M * 0 0 CTTTTAAAAAAAGAACAAATAACACCCTTAAATTCCTCTGAAGTATCATTTTTACTTGTACCAGGAATTTCTTTTGTTGTAATATATTTAGAATGATTTAC GGGGGGGGEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGEGFGGGGGGGFGGGGEEGGEGGEEEGEGGEGGAEF@D@CDDE@FDFEDDDDE@EEC?@ PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2301:3386:87325 99 MtDNA 1965 60 100M = 2135 270 CCTCTGAAGTATCATTTTTACTTGTACCAGGAATTTCTTTTGTTGTAATATATTTAGAATGATTTACGTTACCATATTTTTTTGATTTTATTAGTTTTGA CCCFFFFFHFHHHIJJJJJJJJJJIIJJJJJJJJJJJIJJJJIJJIJJJJJJJJJJJJIJJIJJJJJJJJJJJJJIJIJIIHDDBECEDECDCDCDDDD< PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1109:13191:17203 147 MtDNA 1981 60 100M = 1898 -183 TTTACTTGTACCAGGAATTTCTTTTGTTGTAATATATTTAGAATGATTTACGTTACCATATTTTTTTGATTTTATTAGTTTTGAGTATTCAGTTTTATTT DDCCDDDDDCDDDDDCEEDDEFFDBHFHEHHHJJJJJJJIJJJJIHFGHFIIIHGJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:118:10385:6513/1 16 MtDNA 2048 60 101M * 0 0 GATTTTATTAGTTTTGAGTATTCAGTTTTATTTTTTTTATGTTTAATTGGATTTTCTGTTTATACAACTTTAATTAGCGGTATCGTAAGAAAATCAAAATA DEEFGGFGABGGEEFGEGGFGEGGGGGGGEGEAGGEFGGGGGDGGAGGGGGGGGGGGGGGGGFGGGGGGGGGGGDGGGGGGGGGGGGGGGGGGGGGGGGFG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2108:8075:44880 147 MtDNA 2060 60 100M = 1923 -237 TTTGAGTATTCAGTTTTATTTTTTTTATGTTTAATTGGATTTTCTGTTTATACAACTTTAATTAGCGGTATCGTAAGAAAATCAAAATATGGTATAATTG >>CCDCEDCC@CCDDDCDDBDEGHHHHHGCGGHIGHGGIIHEIGIGHGIFHHIGGHIGIIH@GIIHGGGGHJIIIJJIIHEA4IIIIFHHHHFFFFF@CC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2301:3386:87325 147 MtDNA 2135 60 100M = 1965 -270 AGAAAATCAAAATATGGTATAATTGGGGCCATCCGTGCTAGAAGACAAAGAATTTCTTATGAAATTGCTTTTTCTTTATATGTTTTGTGTATTATTATTC CCCEDDDDEEEDDCCEEEEECCBFFHHHHJJIIJJJIIJIJJJJIJJJJJJJJJJJJJJJJJJJIHGJJJJJJIJJJJJJJJJJJJJHHHHGFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:44:5556:17346/1 0 MtDNA 2177 60 101M * 0 0 AGACAAAGAATTTCTTATGAAATTGCTTTTTCTTTATATGTTTTGTGTATTATTATTCATAATAATGTTTTTAATTTTGTTTCAAAATTTAATTTGAGACT FFEGGGDFGGGGGGGGDGGGEGEGGGEDGFFGFGGFGFDGGGEGGEDFGGGGGG?GGGGEDEFGGGEGGGGGGGGGFFGEGGE?EGEFGGEEGGDFBBAGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:90:16871:20538/1 0 MtDNA 2198 60 101M * 0 0 ATTGCTTTTTCTTTATATGTTTTGTGTATTATTATTCATAATAATGTTTTTAATTTTGTTTCAAAATTTAATTTGAGACTTTTAATTATTTACATCCCATT FGGGGGEGGGGFGGGGGGGGGGGGGGGGGGGGG?GFGGGGGGFGGGGGGGGFGGGGF?GGGGDGDGGGEGGEFGGEGEGGGGGACGGGGFGDGGGFGFGFG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:1:15979:20440/1 0 MtDNA 2341 60 101M * 0 0 TTTTTCTGAAGGTGAAAGGGAGTTAGTTAGAGGATTTAATGTGGAGTTTGCCAGAGTAGCTTTTGTTTTATTATTTTTAAGGGAATATGGAAGATTAATTT GGGGGGGGEFGGEGEEFEEEFFFFFFFFDFFGGEGGGGDGGFGFEFEFFFGGDGDGCEGFGDGGGEEGGEGG=FGGEG?AGGG?DAEBEE=CFAEDDDDEG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2114:8192:26387 99 MtDNA 2360 60 100M = 2687 427 GAGTTAGTTAGAGGATTTAATGTGGAGTTTGCCAGAGTAGCTTTTGTTTTATTATTTTTAAGGGAATATGGAAGATTAATTTTTTTTAGGGTACTTTCTT B@BDFDEEHGHHHIFHIJIIIJBHHFHGFHIJJIIFHCBHGIJJJJGIJJHHJIJJJJJIEIJIEBGHIJJHHGEGHIEGGHGHDDDBCDD>CDDEEDC@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:86:11924:13707/1 0 MtDNA 2394 60 101M * 0 0 GAGTAGCTTTTGTTTTATTATTTTTAAGGGAATATGGAAGATTAATTTTTTTTAGGGTACTTTCTTCTGCTATATTTTTTAAATTTTCAATTTTTATAGCA GGFGGGGFGEFGDFGGFGGEGGGGGGFGGGEDFFGFDDEFGGGFFGGGGGGGGEGDFAFFFGGGGGGEGGEDFDDGGGGGEEEEGFGGEEEGEFGDC:=F5 PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:23:12390:3932/1 16 MtDNA 2447 60 101M * 0 0 AGGGTACTTTCTTCTGCTATATTTTTTAAATTTTCAATTTTTATAGCATTTAGTATTTTTTCATTATTAATTTTTATTCGTAGTTCATACCCTCGTTATCG @A?A?:EE:?E==FGBDGEEGFFFB?DFBDEGG:FD>EGGBFGGFEFFGGEG@GEEFFE:BGFDFG?GDFGEGGGEEFFFEEF?FFEE@BDDFDFFDFDFF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:69:4343:18981/1 0 MtDNA 2510 60 101M * 0 0 TTATTAATTTTTATTCGTAGTTCATACCCTCGTTATCGTTATGATTTAATAATAAGTTTATTTTGATTTAAACTTTTACCAATCTCTTTAATTATATTGTG EECCCDDEEEFFEEECDDADEEEEDFGGFFGGGGAGGGGFEEE:EEEEEEF?GDDGEGGGGGGDGBFGDFFEGGGFGBGCDDFDFGEDGGAGGAGBFEBE? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:106:17241:4836/1 0 MtDNA 2631 60 101M * 0 0 ATTATTAATCAAGTTTATTTTTTAGACATTTTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAA GGGGGGEEFGGGGGGGDGGGGGGGEGGGGGGGGAFGGFGEGDGGGFFGGFFEFGGGGGGDGGFFGGGGGFDGG?ACEEFFGDGEDFDD=FBAEEEFBF?FF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:118:1597:14954/1 16 MtDNA 2633 60 101M * 0 0 TATTAATCAAGTTTATTTTTTAGACATTTTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAAT FGGGEEGGBECG?GEGGGGGFGGBGEGGBFGGGEFGGFFGEAEDAEBGDGGGEGFGGGGFGGGBGGEEGDFGAGGFGFGFDFGGGEGFGEGGGEFGDGAGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:30:9587:17311/1 0 MtDNA 2647 60 101M * 0 0 ATTTTTTAGACATTTTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTA GGGGGGFFEDGGEGEGFGGGGGGGG;EEEEFGGGGGGGEGGEGGEEGFGEGGGGEGDGDGCCEEBFDFFFGGGDGGGGGGFGGDGGGFGGFGGGGGGFDGC PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:90:16079:8007/1 16 MtDNA 2653 60 101M * 0 0 TAGACATTTTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGA GEEBEA5GFGGGGGAEGGGGGGGGGFGFDFGGGGCGDG>GEGGFGGAGGGGDFDGGGGGFGFFFFBFFFDFGGGGFGGGGFGGFGGG?GDGDDGGGGEGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:48:1800:14293/1 16 MtDNA 2661 60 101M * 0 0 TTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGAGTATTTAG FFDFBAC?ACC>E5FFFFFFFEFACCACE?C@ADE=FEFFFFFDFFFDFFFFFFFFFDEBFFFFFB?FFDF?F=ADFFFFFEEAEEDEEEFEFABDGGDG= PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:83:16330:7076/1 16 MtDNA 2681 60 101M * 0 0 ACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGAGTATTTAGATATACAAATACTTTACCAT BEE@@>?FD:FCFFBFEFDFFFDFFBFFFBFFFF=FFBFFFFFFFDFBFFDFFFD=D=:FFDEFFFDFF?FFFFE=EEDDDEEEC@?ACCCBD= PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2114:8192:26387 147 MtDNA 2687 60 100M = 2360 -427 TTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGAGTATTTAGATATACAAATACTTTACCATTAAGG ECDEEFFFFFFHHHHFGHJIIIGHEEIJIJHHJIIJJHGJJJJJJJIIJIIIJJJJIIJIJJJJJJJIFIIIIJJJIJHJJJJIHFJGHFFHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:23:17204:14243/1 0 MtDNA 2720 60 101M * 0 0 TTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGAGTATTTAGATATACAAATACTTTACCATTAAGGTCAGTAATTTCTATTTTTACTTTTATTGTTCTTT ;;;9;:===:C;CCA=?DDDDDDDD6>C>>@@??B:D=DDDDDDDDDDD=DDDDDDDDDDDDDDD=DADDDABBBDDD=5DCD?CC>AAABCDDDD=CCCA:AA?CDDDDDDABDBDDB?DC=C@??ACDD:CA9ABDC=>CCCDD=D8>BBBBBDC5BBCD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:73:15087:6864/1 0 MtDNA 2787 60 101M * 0 0 TCAGTAATTTCTATTTTTACTTTTATTGTTCTTTTAACTTGTTGTTTTGGAGGTTATTTTACTTACTCTTTTTGTCCTTGTGGAATGGTTGAATTTACTTT ==?;B;C>=;CC?CC?>:8CA=?C:;==CBD?DDCB?B?DDBDD@ABA>ABDDBBCDAD>>CDDCDCDDDCD@A>>CDDDCDA5BC=D5DDB:B5@=>CCD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:41:4283:1044/1 16 MtDNA 2804 60 101M * 0 0 TACTTTTATTGTTCTTTTAACTTGTTGTTTTGGAGGTTATTTTACTTACTCTTTTTGTCCTTGTGGAATGGTTGAATTTACTTTTGTTTATGCTGCTGTAG FBCCFDEEGEEAEGEEEEFEGEGGGFGFGGGGGFFFAGGGGFFCFFDEGGGGFGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGFFGGGFGFGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2212:11575:96550 99 MtDNA 2839 60 100M = 2949 210 GTTATTTTACTTACTCTTTTTGTCCTTGTGGAATGGTTGAATTTACTTTTGTTTATGCTGCTGTAGCGTGATTAAGTACTTTGTTAACTTTTATTTCAAG @@@DFFFFHHHHHGIGHIIJJJIIIJIIDHGHDHHGEGHIEIJJIGIIIJGHGGGIIIGEEHEDGGIDFD;7>CEECACDCDCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:83:6736:2885/1 0 MtDNA 2866 60 101M * 0 0 GTGGAATGGTTGAATTTACTTTTGTTTATGCTGCTGTAGCGTGATTAAGTACTTTGTTAACTTTTATTTCAAGAGAAAAATTTTCAGTTTATATAAGAAAA EEEEEFFFFDDGEGFGGGGGGGGFEEEEEGGEBGEGEEFGGEGEEEEEBBCEDEEEEEEEEEEGEEEEEEEEEEEGEBEEEEEEEEEEEEEEEEDEEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:62:11372:10324/1 0 MtDNA 2896 60 101M * 0 0 CTGCTGTAGCGTGATTAAGTACTTTGTTAACTTTTATTTCAAGAGAAAAATTTTCAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGA F?FFBDB?=D@:@@@CC:>5:;A;;DC>DDDD:ADDA@>B=DA?D?DDC@>=?AD?DBCBBDDCDBCBDB>>DA=??5?B?C?@?@=>@CBBBBC=>@>>B PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:91:13282:2427/1 16 MtDNA 2915 60 101M * 0 0 TACTTTGTTAACTTTTATTTCAAGAGAAAAATTTTCAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGAATGCTATTAATTGAAATCG CEEEEEEEEEEEEEEEEBBEEEEEEEEEEEEGGEEGEGGEGEGF>EFGEGEEEEEFGFGEGEGGGDEGEGGGFEFGGGGGGGGGGFGGGGGGEEGFGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:53:2586:12076/1 0 MtDNA 2916 60 101M * 0 0 ACTTTGTTAACTTTTATTTCAAGAGAAAAATTTTCAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGAATGCTATTAATTGAACTCGT GGGGGFGGFBGGGGGGGGGGFGGGGFGGD8EEEFFGGGEGGGGGG?GGGFFGGGGGFGGGGG?GAGEGGEDAFGFFEGGGEEEBGEAEEEEEEDE=::?>C PG:Z:SNAP RG:Z:266-1 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:2212:11575:96550 147 MtDNA 2949 60 100M = 2839 -210 TCAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGAATGCTATTAATTGAAATCGTTAGAGAATTTTCTCGTCCACTTGCTTTAACAG ACDDDDDDDDEEECDEFDFFFHHHHHHCJIJIJIJJIHG@GHCJJJIHGGCIGIJJIIGIIJIHHGGJJIIIDIGGIGGEIIGIHG@HGHHFFFFFF@C@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:54:12110:15974/1 0 MtDNA 2950 60 101M * 0 0 CAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGAATGCTATTAATTGAAATCGTTAGAGAATTTTCTCGTCCACTTGCTTTAACAGTG GFGGGGGGGGGGDGGGGGGEFGGGDFGFGGGGGFGGFEEGFFGGEGEEBGFEGFGDGGFGEGFGGEEEDEEEEAEEEFEEGEEEEEEEEEEEEEBCCADA? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:80:9039:4187/1 16 MtDNA 3058 60 101M * 0 0 CAGTTAATATTACTGTTGGTCATTTAGTTAGAATAATGCTTTATCAAGGATTAGAATTAAGAATAGGTGATCAGTATATTTGATTATCAATTTTAGCCATT :@?A@@ PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:24:5066:4097/1 0 MtDNA 3060 60 101M * 0 0 GTTAATATTACTGTTGGTCATTTAGTTAGAATAATGCTTTATCAAGGATTAGAATTAAGAATAGGTGATCAGTATATTTGATTATCAATTTTAGCCATTAT DGGGGGGGEAFFFFFFFFFFEAEEEEF?FEFEBFFFFGBBDEEEBGGEE=FFDFFGGEGEFFEFFAFDFEBFAEEDGEFGEEBGEGGEGEGDEDEEEFBEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:105:7673:2329/1 16 MtDNA 3249 60 101M * 0 0 GTTAACTTAAGTTTTAAAGTGCCAAACTTTTAATTTGGAAATGGTGGACCACATCTTAGTTGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGA EE5DEE5FFEEFBAEFD@?==D5:C?F5FFFGGBGFFFDFF=EEEECBEEBDE:DD?:GGGDGGEGGGGGEGGGGDGGGGGFGGGGGGGGDGFFGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1113:17155:40031 99 MtDNA 3295 60 100M = 3421 226 GACCACATCTTAGTTGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGT @BCFFFFFHHHHHHIJJJJJJJJJJJJJIIIEHIJJJJJEHIJJJJJJJJJJJJJJJIJJJHIJJIIGHHGDFBCAEEEEEDDDDDACEEDDDEDDEDDE PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1305:16341:33179 99 MtDNA 3300 60 100M = 3308 108 CATCTTAGTTGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGTTCTAG CCCFFFFFHHHHHJJJJJJJJIJIJJH>HIIJJJHIIJEGJIJJJJJJJIIJJJIJIJIJJIJJJJHHADFFFFFDCECEECDDEDDDEDDEDDDDDEED PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1305:16341:33179 147 MtDNA 3308 60 100M = 3300 -108 TTGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGTTCTAGGTTAAATC CEEEEEDEDDDDDDCDDDC@EEEDFFFFFFHHIFJIJJJJJJJIJJJIHHJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFC@C PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:61:8761:13140/1 0 MtDNA 3309 60 101M * 0 0 TGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGTTCTAGGTTAAATCCT FFFFF?EGGGGD=FGGFFGGDFDFFGGGGDFDGGGD?BDFFGBGGGGDGFDFFFFGGBDDEFEFFGDEFEEGBGEBBC?BBBBBCADBEDCB:CA?@B@@: PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:72:9498:11197/1 16 MtDNA 3343 60 101M * 0 0 CAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGTTCTAGGTTAAATCCTGCTCGTTTTTGATTGTTTTTATTTCTTTATTTAC EED=DF?>>A?A=>5@?CDBBFB=EFFEEDBBFDDFEDFFFFFDDFFD;FF?FEDFDFDFFEEEBDEFDFDDBFFFDFDFEFFFFFD>EFAB>EAADDDCB PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:30:13606:9054/1 16 MtDNA 3373 60 101M * 0 0 AAAGCAAGTCTTCTAAATTTGTTCTAGGTTAAATCCTGCTCGTTTTTGATTGTTTTTATTTCTTTATTTACCTTGTTTTTAACATTATTAAGAATTTTGAC EA5B??:EEEEEEEDEDE=DBBEEB=FBEDEEEEEFDEGGEEGGFEEGA5DEGEGGEAEGGECB=FEFF=FFGEGFGFFDGFFFFDEFGFDGGGGGFGDGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1113:17155:40031 147 MtDNA 3421 60 100M = 3295 -226 ATTGTTTTTATTTCTTTATTTACCTTGTTTTTAACATTATTAAGAATTTTGACTAATAACGTTATTGTTTGATGAAGAATTTTTTTATTGATAACTGTAG CDDDDDDDDDDDDEEEEEEEDCFFFHHHHJIJJIJJJJJJJJJJJJJHGDIIJJIIJIHJJJJIJIJJJJJJJJJIGIIJJJJJJJJHHHHHDDFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:79:5551:14500/1 16 MtDNA 3442 60 101M * 0 0 ACCTTGTTTTTAACATTATTAAGAATTTTGACTAATAACGTTATTGTTTGATGAAGAATTTTTTTATTGATAACTGTAGTTTTTATTCTATTAAATAAAAG 5DGEGGGGEGGGEGFGGFFFGFGGGGGGGGEFGFGGGEFGGFGGGGEDGGFGGGFGDEGGGEGEGFGGGGGFAGGGGGGGGGGGGGCGGEGCGGGGGGGGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:118:9818:14692/1 0 MtDNA 3448 60 101M * 0 0 TTTTTAACATTATTAAGAATTTTGACTAATAACGTTATTGTTTGATGAAGAATTTTTTTATTGATAACTGTAGTTTTTATTCTATTAAATAAAAGCAGCAA GGEGGEGGGEFGGGBGGFGGGGGGGGFGBFEGGGGFFGGGGGGEGGGGGGEGEGADGGGEGFEGGGGGBGEFFEFGGFGGGGEGGGGGG?GFGAGEGGGCB PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:50:8060:11985/1 0 MtDNA 3466 60 101M * 0 0 ATTTTGACTAATAACGTTATTGTTTGATGAAGAATTTTTTTATTGATAACTGTAGTTTTTATTCTATTAAATAAAAGCAGCAAGAGATATACCAGAATTTT EEAEAC=D=D:AACA@;@=@>@<@@DDDD?CA5C?DDDDDCABCADD=DDDDDDDA=ADA?>>AADDACCCCBBCAD?CBCDC?B:=BD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:56:13819:18497/1 0 MtDNA 3542 60 101M * 0 0 GCAGCAAGAGATATACCAGAATTTTTAATTATTTTGTTATTCAAGAGTCTTTAGGTTTATTATTTCTTCTTTGTAGAGGAGGTCTATTACAATTTTTTATT GGGGGGFGGGGGFGGGGGGEGGGGGGGGGGGGGGGGGGGGGGGGGEGFGGGGGGGEGGGGGGGGGGGGGGGGGFGGBEGEGEEGEEGFEFEEEFEGEEFEA PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:69:5893:18114/1 0 MtDNA 3759 60 101M * 0 0 TATTTTATTACAAATTTTTTGGTTAAGATCCGTGTATATTTTGTTATTTGGTTTATTGATTTGTTATGTTCAAATTTTTGTCATAAAAAGTTATAAAAATT FGGGGGGGGGGGFGGGGGGGGGGGGFGGGGGGGGDAGGGGFFFEEAGGGDGEDGDGGGDGGGFBGDFGFGGDAGGGGGGGGGGGAEFGGFEFBGGEGDCCG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:67:1744:8605/1 0 MtDNA 3773 60 101M * 0 0 TTTTTTGGTTAAGATCCGTGTATATTTTGTTATTTGGTTTATTGATTTGTTATGTTCAAATTTTTGTCATAAAAAGTTATAAAAATTTGTTAATTATTTCA GGGGGGGGGGFGGEGFFFFFBDEBEGGGGFG8DGGDE@EEEEEE?GGGFFG?GBEGFEGEEGGGGFCCAGEEFFEDDBEFEGEFEEGGGEGEEGEEAFGFB PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1310:8213:55897 99 MtDNA 3943 60 100M = 4215 372 ATTTATTACTTTGTATTAATAGTTTTATTAATTTCTAAGTTTTCTAAAACTAGGGGTTATAATTTTATTAATTGAGAAACAACATTAGTATTTTTAAATA CCCFFFFFHHHHHFHHIJJJJJJIJJJJJJIJJJJIJJJHIIJJJJIJJJIJIJJJGIIIJHIIJJIIJJIIIJEGGIJJIIHHHHHHCEFFFFFDEEEC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:77:12526:7315/1 0 MtDNA 3981 60 101M * 0 0 GTTTTCTAAAACTAGGGGTTATAATTTTATTAATTGAGAAACAACATTAGTATTTTTAAATATTCCATTTAGAGTTTCATTTTTTGTAAAAATTTTCTCAT EEEEEEEDEBDDFFFFEFAEFFFFFFFFFFFFFFFFFDFFEFFFFFEFFDEFEFEFFFFFFDEFFFEFFFAEBE@EEFEFFFFFEBEEFEEDFEEEE?EBF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2311:10246:30315 99 MtDNA 3984 60 100M = 4165 281 TTCTAAAACTAGGGGTTATAATTTTATTAATTGAGAAACAACATTAGTATTTTTAAATATTCCATTTAGAGTTTCATTTTTTGTAAAAATTTTCTCATTG CCCFFFFFHHHHHJJIIJJJIJJJJJJJJIJJIJJJJJJJJJGJJJIBGIJJJJJJJJJJJJJIJJJJIJIHIJJEHIJJHFFFEDDEEEDDDE@CCDEC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:12:4535:13471/1 16 MtDNA 4089 60 101M * 0 0 AATTTTTAAATATGATAGATTCTTTACTCTATTTTTGCTTTTTACAATATTTTTATCTGTATTGGCATTTAGATTTTGATTAATTAACTTGAGAATGAAAA GGGGDADGFGFEGEBEFEFFFEEEEBEBC@EGGGGGEGFGGGGFGFG=ADEC:DAFFE=FFFBFBF?FFD:FDFFFDFADE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:84:1391:9200/1 0 MtDNA 4874 60 101M * 0 0 CTTTTATAGGTTATGTTTTAGTTTGAGCTCAAATAAGATTTTGAGCAGCAGTAGTTATTACTAGACTTTTAAGAGTTATTCCAATTTGAGGGCCAACGATT GFGGGFFGDDEBEEEFFFFEFFFFFGGGGGGGGEDGFGGFGGGGEGBGGGGFGGGGAG?EFFDGGFGGGG:EEAD=ECEEEGFGGFFFFEA?CEABB7BB@ PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:2:107:7520:19689/1 0 MtDNA 4896 60 101M * 0 0 TTGAGCTCAAATAAGATTTTGAGCAGCAGTAGTTATTACTAGACTTTTAAGAGTTATTCCAATTTGAGGGCCAACTATTGTTACTTGAATTTGAAGAGGTT BGGGGADGGGGDGGFFAGGF:FFFFGFGFFFACDEFFFEBGF?GGGFGBEEC5DED==D??ECEEEFGGGFFEFBD=BAEDDBFBD:?BDEEDB?===D@? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:31:10465:15263/1 0 MtDNA 4937 60 101M * 0 0 GACTTTTAAGAGTTATTCCAATTTGAGGGCCAACTATTGTTACTTGAATTTGAAGAGGTTTTGGTGTTACAGGGGCAACATTAAAATTCTTTTTTGTATTA GGGGGGGGDGGGEGGE=EEEDGDGGFGDGGGBGFGGGGGEGDGEEGEGGGGEDEGEEE=EEEDE=FAEFAEEFDEBGFEDEEEEBEBEEBEGEE7C:=5AB PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:29:14125:10562/1 16 MtDNA 5109 60 101M * 0 0 AACATCTAGGTTATATTGCCACGGTGATTATGATAAAGTTTGTTTTAGACCTGAGTACTTAGGTAAAGATGCTTATAATATTGTTATTTGATTATTATTTA ECGDEGEGEGB?FBGFEGGGCGEGDGFGFG?GGGGFEGG6GGGGDGGGEGGEEEE=EGGGEGGGGFBEGEGGBFGGGGDFFGFFGGGEDGGEGGGGGGGFG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:37:12921:1122/1 16 MtDNA 5174 60 101M * 0 0 AAGATGCTTATAATATTGTTATTTGATTATTATTTATTGTGTTAAGGTTGATTTACCCGTTTAATTTAGGTGATGCAGAGATGTTTATTGAAGCTGACCCT DAB=?B=BGGEGBEDGEGEGGGGDGEGGFAFBGEGGG5EGGEBGGGCE?E?GGECGDEFGGEGGGGFGGDGGGFGDGGGGFFGGGGDGFGGGFFGGDFFFF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2215:6882:11082 99 MtDNA 5381 60 100M = 5391 110 GGATCGTAACATTTTATTTTTTTGCTTTAGTTAATAATTATACCTCTTGTTTAACCAAATTAAATAAATTTTTAGTATTTATATTTATTATCTCATCTAC @@=DDDDDFHFDHIIHIJJJJJIJIIIJJJGIGIJGJJJIJJJJJIIJJH=FGHJICHHIJJGIJJIIHEEHHHF@EFFFFEEEEEEEEEFDDDCCCDD: PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2215:6882:11082 147 MtDNA 5391 60 100M = 5381 -110 ATTTTATTTTTTTGCTTTAGTTAATAATTATACCTCTTGTTTAACCAAATTAAATAAATTTTTAGTATTTATATTTATTATCTCATCTACTATTTTAAGA =CCCCBBBEEHEHCFIEAGGHIFGC@IGGHFCIIIGIGD@IHGIGIHEEIHFGIIIIIDIIIIIIIIGFC:IIIIHECIGEFECCCCA:EE?EF=A@ECFFFFD?EEEEDEE:EFFEFEEEBAC;FECCDBDEEEBA??F?DFAFFFEEDDE=DDAD: PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:96:18019:19876/1 16 MtDNA 5769 60 101M * 0 0 TTTTTAAATTTGGACTATACGAATTATTTATTTTTACACTATTTTCTGTGTTATTTATTTCTTTTGCTTGAGGTAAGGATATTGCTATAGAAGGTTTAAGA GEBBFGGFEEGEE=EBGFEGGEEEEADDGGGEEEEBEAFFEFFDEDGG?GGGFGFG@DGGFGGBGFGGEGFFGGGGGGDFFGBGBGGGGGFGFGGFDDFEF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:28:5015:7424/1 0 MtDNA 5798 60 101M * 0 0 ATTTTTACACTATTTTCTGTGTTATTTATTTCTTTTGCTTGAGGTAAGGATATTGCTATAGAAGGTTTAAGAGGTTACCATAATTTTTTCGTTATAGACGG GGGGGGFGDGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGGGGBFFFFFGGGGGGGGGGGGGGGBGGFGFEFFCFAEFFDDGFGGFGFEGEGFFDFDDFF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:30:5276:1934/1 0 MtDNA 5801 60 101M * 0 0 TTTACACTATTTTCTGTGTTATTTATTTCTTTTGCTTGAGGTAAGGATATTGCTATAGAAGGTTTAAGAGGTTACCATAATTTTTTCGTTATAGACGGGTT GGGDGGGGEGGGGGGGFGGGEGGGGGGGGGFGGGGGGGGGGDGDGFCFCFGGGGGGDGDGGGDGG=EDBEFAFDEDEFBEGGGGEGEGEFCECEC?EEECC PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1104:14433:23059 99 MtDNA 5853 60 100M = 5996 243 CTATAGAAGGTTTAAGAGGTTACCATAATTTTTTCGTTATAGACGGGTTTAAATTCGGAGTAATTTTATTTGTGTTTAGGGAGTTTATATTCTTCTTTTG BCCFFFFFHHDFHIJJIHJFHJJJJJJJJJJJJJJJIJIJJJIJJII@FIHIIIIIJJHH?AEHHHFDFFFFFEA6@>CDD;?EGGGGGGFGGGGFEEEEF;EFEEGGGGFGGGGGGGGGGFGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:1:76:4139:2647/1 16 MtDNA 5966 60 101M * 0 0 TTTTTTGATGCTGATCTAGTACCAGTACACGAGTTGGGAGAGACTTGATCACCTTTTGGAATGCACTTAGTTAATCCATTTGGTGTACCGTTATTAAATAC EBBEEEEEEEE@B@BEB@@B?BC?BB@B?DEEEDEFDBEEEB=CCCE@EE@FFFFDECEEDEDEBEFFEFDFFFEFEAFDFFEFCFBDFFFFFFAFGGGBG PG:Z:SNAP RG:Z:266-1 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1104:14433:23059 147 MtDNA 5996 60 100M = 5853 -243 GAGTTGGGAGAGACTTGATCACCTTTTGGAATGCACTTAGTTAATCCATTTGGTGTACCGTTATTAAATACAATTATTTTATTAAGGAGTGGTGTTACAG CDDDCCAECEC@EEFFFFFDAHHGJJJJJIJIJJIJJJJJJJJJJJJIGHHGJJJIGJIJJJJJIJJIIHJJJJJJJJJJIJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:99:8553:16567/1 0 MtDNA 6020 60 101M * 0 0 TTTGGAATGCACTTAGTTAATCCATTTGGTGTACCGTTATTAAATACAATTATTTTATTAAGGAGTGGTGTTACAGTAACTTGAGCACATCACAGATTACT :FDGBFGFGDDGFDGFFGGFBGGDGFFFF=EAEEEDEEAEFDFDFGFG?GG?FEGGGFGGGGGFFDFFEFEAFFFGEGGGGAGEFGEFGGFGGECBEDDFG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2201:8667:82934 99 MtDNA 6028 60 100M = 6185 257 GCACTTAGTTAATCCATTTGGTGTACCGTTATTAAATACAATTATTTTATTAAGGAGTGGTGTTACAGTAACTTGAGCACATCACAGATTACTTAGAAAT CCCFFFFFHHHHHJJJJJJJJFHGHIIJIJJJJGJJIIJJJIIJJJJJIJJIIJJJJBFIBFGHIEIGEGHJJJJJHHHHGHFFFFFDECECEEEDDDDC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:83:16211:16889/1 0 MtDNA 6029 60 101M * 0 0 CACTTAGTTAATCCATTTGGTGTACCGTTATTAAATACAATTATTTTATTAAGGAGTGGTGTTACAGTAACTTGAGCACATCACAGATTACTTAGAAATAA EAEEBGFGGFGGGGEFFFAFEFFGEGFEDGDEECCGFDFBGGEGFGG@FGCBFDDFDFDBBDDBBBEBEFBAEEBFBDFEEGEEEB=B@BBEB?BAABDBE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:8:8735:6148/1 16 MtDNA 6076 60 101M * 0 0 ATTAAGGAGTGGTGTTACAGTAACTTGAGCACATCACAGATTACTTAGAAATAAAAGATGTACTAATAGTATAATTTTAACATGTTTATTGGCAGCTTATT DB:DDDB?D:BDCDDDD?DDDD=DADDADDBDDDDBDDDDDDADDDBC>CDDADADDD:DBCCBDDDDADDDDDBD5DDDDE?DEE:EFFEEEBDD;EEE: PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2201:8667:82934 147 MtDNA 6185 60 100M = 6028 -257 ATTCAATTAATAGAGTATATAGAAGCTAGATTTTCTATTGCAGACGGAGTATTTGGAAGGATTTTTTATTTATCTACGGGATTTCACGGAATTCATGTCT CCACEEFEECDCDEEEEEEEDEFFFFFFEGHHHHIJJIGJJHHDJIJJIHHJJJJJJJIGGJJJJJJJIJJJIIHGJIJJJJIIIGJHGHGHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:105:1340:12432/1 0 MtDNA 6235 60 101M * 0 0 ATTTGGAAGGATTTTTTATTTATCTACGGGATTTCACGGAATTCATGTCTTGTGTGGTGGTTTGTTCTTAGCATTTAATTTTTTACGTCTTCTAAAAAATC FFFD@DGGEEEFGGGGGGDFGGGGGGGEGGFEFFEEDEE:EADDEFF5FBEE=EEDDADDB?DE=><:6>EEDEE:D5EDEEBB5:ECAC:?B:CEBEEEC PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:54:12468:5717/1 16 MtDNA 6304 60 101M * 0 0 AGCATTTAATTTTTTACGTCTTCTAAAAAATCATTTTAATTATAATCATCATCTGGGGTTGGAATTTGCTATTTTATATTGACATTTTGTTGATGTAGTGT BBB:DDC?CBCB@AB:BDDDD@C?>CACB::?DCD=CC:?A=D:DB?DDAD:?CCCD=D:DDDDA?ADDDA;C:AAC?=A??:@CCB PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:35:14137:12613/1 0 MtDNA 6539 60 101M * 0 0 CTTTTTAAACCTATTTATTTTTTATTATTTACAGTGATGTTTAGGTTTTTAATTTTTAATAATTTTTCATGAGGCGGACTATTTTTAGTATTAGATTCATA GGGGGGGGGGGGDGGGGGFGGGGGGGGGGFFGFGDGCGGECFFFF?FEFDEAGGGAGGGGGGGGGGDGFGGEFFGGGEEGEGDGGE@GEEGEEFEEAEED@ PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1210:8873:86961 147 MtDNA 6541 60 100M = 6488 -153 TTTTAAACCTATTTATTTTTTATTATTTACAGTGATGTTTAGGTTTTTAATTTTTAATAATTTTTCATGAGGCGGACTATTTTTAGTATTAGATTCATAT CACCCCCCCDA@CCACAIGIGH@;F9EEFC1EIHHCEHGBCIHFAGD9HD?9DCFFCGCCA@A,ACGEIIGGGEDE?F?DFABD7D@@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:80:12688:9364/1 0 MtDNA 6564 60 101M * 0 0 TATTTACAGTGATGTTTAGGTTTTTAATTTTTAATAATTTTTCATGAGGCGGACTATTTTTAGTATTAGATTCATATAGATTTATTTTATTGATTGTTATA GGGEGAFCDDFAFDFEECDE@EEDEEFFFBDDDD:A?AC?GDGEFFGFEACEB:C=EEEE>:C@BEEEE=DEFA?DFEE?DE??EFC>5B;?AAEDAD9D= PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:83:3138:12153/1 16 MtDNA 6612 60 101M * 0 0 GCGGACTATTTTTAGTATTAGATTCATATAGATTTATTTTATTGATTGTTATAAGATTATTTATTTTAGGTATTATTGTTATTAGAGAAAAAAATAATAAT GGGFEEGFEEEBEDGGFGFFGGGGGFGFGGEGFGDGGGGGGAGGFGGGGGGGGGGGGGGFGGGFGGGGGGGDGGGGFGGBFGGGGFGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:59:15380:6072/1 16 MtDNA 6635 60 101M * 0 0 TCATATAGATTTATTTTATTGATTGTTATAAGATTATTTATTTTAGGTATTATTGTTATTAGAGAAAAAAATAATAATTTATTGATTTTATCTGAAATTTT EDGEEBEE@EEGEEEEEEEAEEE?EEEEAEBFEEFEEEGGGEEGDEEFEDDGFEECGGEDGEGEDGEGDGEGFGEGGGFEECDCD;G=GFFAGGGGGGGGF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2109:14592:38248 73 MtDNA 6659 60 87M1I5M1I1M1I4M = 6659 0 GTTATAAGATTATTTATTTTAGGTATTATTGTTATTAGAGAAAAAAATAATAATTTATTGATTTTATCTGAAATTTTAGTATTTATTCTGTCTCTTATAC CCCFFFFFHHHHHJJIJJJJIIICHJJIJJIJJIJJJJJJJJJJJJJJIIJJIJJJJJJIEIJJIIJJJJIGGHJGHHHFHHHFFFFFFFEEEEEEDEDD PG:Z:SNAP RG:Z:000 NM:i:6 +BS-DSFCONTROL03:121:D0M8KACXX:5:2109:14592:38248 133 MtDNA 6659 0 * = 6659 0 AATAAATACTAAAATTTCAGATAAAATCAATAAATTATTATTTTTTTCTCTAATAACAATAATACCTAAAATAAATAATCTTATAACCTGTCTCTTATAC CCCFFFFFHHHHHJJJJJJJIJJJJJJIJJJJJJJJJJJJJJJJJJJJJJHIJJJJJJJJJJJJJJJJIJJJJHHHHHHHFFFFFFFDDECEDDDDDDDC PG:Z:SNAP RG:Z:000 +EAGLE:1:39:10037:14104/1 16 MtDNA 6823 60 101M * 0 0 TATAATTCTAGGTTATGGTTCTCAAATTGAAAAAATTAACTCCTCTTATTATTTAATATTTTATGCAGCTTTTTGTTCATTCCCATTTTTATTTGTATATT E?F?FDFGF?FGAGAGFFFEDGGDGGGFGFGFFF?FG?EGGDDBDBAF>EFEGGGGCGDGFGGFGGFGGGFGGFGGFGGGEDGGFGGDDGFFGEGFFGFDF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:51:9949:15014/1 16 MtDNA 6862 60 1M1D6M1I87M1I5M * 0 0 CGATCTTTATTATTTAATATTTTATGCAGCTTTTTGTTCATTCCCATTTTTATTTGTATATTTTAAGAGTAATTTTTTATTGGTTTTTTCTTACTTATTAC EEEEEEEBEEEEEE=EEEEEBEEEEEEEE=C?EEDDEEDEE?EEECCCCEDDEEEEBEEEEEEEEAECEBACAC; PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:1:86:3647:16273/1 16 MtDNA 6985 60 101M * 0 0 TTTTATTCTTAGTTTAAGATTTATAATAAAATTTCCAATTTATTTTTTACATCTTTGATTACCTAAAGCTCATGTAGAGGCTCCTACAACAGCTAGAATAC FDC@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:102:7363:18384/1 0 MtDNA 7214 60 101M * 0 0 TGTGTGTTTCAAAGAGATTCAAAGGCTTTAGCAGCTTATTCTTCAGTCACTCATATAAGGTTTTTATTATTATCACTGGTATTTATCACAATGAGTAGTAA D:BDDCEDEEBFFFFGGEGGGGGGGGGGG:EEEEEGGGGFGFGGCGEGGFGDGGGGGEEGBEEEEFGGEGEAFE:FFDFCGGGGGFFADDFFFFFAFFEEF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:38:3103:6670/1 16 MtDNA 7304 60 101M * 0 0 ATGAGTAGTAAAATTAGAAGAGTTATGCTTATGCTAGCACACGGTTATACATCTACACTTATGTTTTACCTTATTGGTGAATTTTATCATACAACTGAAAA FGGEGGGGGGGGGAGGGGFGGGEEGGGGGGEEGGGGGGFGGGGGGGGGFGGFFFDFEGGGGFGGGGGEGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:4 +EAGLE:1:55:13359:5768/1 0 MtDNA 7379 60 101M * 0 0 GGTGAATTTTATCATACATCTGGGAGGCGTATAATTTATTTTATAAGTAGATTTTTTAGATCTAGAATAATTATAGGTATTTTGTTTTCAGTTGTATTTTT FBFEFFFADEGBGDGDGGGGGFFGGGGFGDGEBBGGGFFGGGGAGEEBFFFGEGFGGEBEGGGBGEEGDFDGFGEGGEAGFGGGDGFEEBCEEECEFGGG? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:40:8204:14676/1 16 MtDNA 7463 60 101M * 0 0 TTTTCAGTTGTATTTTTATCAAATAGGGGTGTACCACCTTCTTTGTCATTTTTATCAGAATTTTTAGTTATTTCTAATAGTATATTAATTAGAAAAAGTAT DDBEBGED?FGGECGGECDE;FGEGGFGGDGFDFFCFF>FFGGGGGDGGGEGGGGGGGGGFDGGDGGD?GGDGGGGGGGGGFGGGGGGGEGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:17139:67717 99 MtDNA 7469 60 100M = 7536 167 GTTGTATTTTTATCAAATAGGGGTGTACCACCTTCTTTGTCATTTTTATCAGAATTTTTAGTTATTTCTAATAGTATATTAATTAGAAAAAGTATATTTG CBCFFFFFHHHHHIJJJJJJJJJEHGHIIIIJIIJJJJJIJJJJJJJJJIIIIHIJJIJIIGIJJJJJJEGHGGCCEHHHHHHHFFDFDDCD;CCBDDE> PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:17139:67717 147 MtDNA 7536 60 100M = 7469 -167 CTAATAGTATATTAATTAGAAAAAGTATATTTGTAATAATTTTTATTTATTTTGTAGTATCTTTTTACTATTCTTTATTTTTGATTACAAGATCTTTAAT DEEEEFFFFFFFFHHHHHHJJJJIJIJJJJJJJJJJJJJIJJJJJJJJJJJIJJJJJJIIJJJJJIGJJJJJJJJJJJJJJJJJJJIHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:57:7347:8572/1 0 MtDNA 7620 60 101M * 0 0 TTACAAGATCTTTAATAGGAAAAGGTTACCATAATTTTAATACTTGAAATGTAGGGTTTTCAGCACCATTAGTCTTAATAATATATAATGTATTTTGATTA FEFFDEBE??EEDEAFEDFDFFFDEBBDEEC?CCD=EEEBFFEEFF?FF=?:?ACE?BCEFFFFDFEFDEFEEEDEBDDEEEDEEEDDED@B=ABEDDBEB PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2114:12015:97588 99 MtDNA 7885 60 100M = 7994 209 GATTAGAGAGATCTAATCATAAAGATATCGGAACTCTTTATTTTATTTTTGGACTTTGATCTGGTATGGTTGGTACTAGATTTTCTTTATTAATTCGTTT B@@FFDFE>FFHHIJJIGIIJJIGHGJJJFIIIJJJJJJHJIJJGIJJJIGHIGIIJJIIIJIGCGHGDEHIE?CEFFFEFFEDEEEEDEDCDEEDDDDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1314:9064:66647 99 MtDNA 7912 60 100M = 8034 222 TCGGAACTCTTTATTTTATTTTTGGACTTTGATCTGGTATGGTTGGTACTAGATTTTCTTTATTAATTCGTTTAGAATTAGCTAAACCAGGTTTTTTTCT BB<1:=@BFHFH>CFHB>EC;=;@EEB;@>ACD555=?=BDC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2114:12015:97588 147 MtDNA 7994 60 100M = 7885 -209 TAAACCAGGTTTTTTTCTTAGGAATGGACAGTTGTATAATTCAGTTATTACAGCTCATGCTATTTTAATAATTTTTTTTATGGTAATACCTACTATAATC CBC@DBCBDDDDDEDEEEEFFFFC?BDAFHHEIJJJJIHGGGIIGIIIIHJIIIGFJIIGJJJJJJIIGIIIJJJIIIIJIJJJJIGFGHHFFFFFFC@C PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1314:9064:66647 147 MtDNA 8034 60 100M = 7912 -222 TCAGTTATTACAGCTCATGCTATTTTAATAATTTTTTTTATGGTAATACCTACTATAATCGGTGGTTTTGGTAACTGATTATTACCACTTATGTTAGGAG :::@@B;5AA:@:@5:A>>A>;B@@@>DA>@?6AC;7C@=8BD94:<<:H@BBEHHGHHGGI?GEH>?FADFGIGIICGGEGIBAHGIICFHEEC>DGEHGGHFF@BDEE@CACCCCDDDDD:::>A>@A>@;EEEEDDEEEDAB@DDDDDB;?=?:IEECAEC;@CBDF==FCFB?FBBDGIJIIGG@DHHG9FGIBHFBEDDFEDD?BDDDDDDCB5>>CEDCDCA PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1207:2101:11733 147 MtDNA 8633 60 100M = 8591 -142 ATCTACACTTTATTTAACAGGAAAAAAAGAAGTTTTTGGTGCTTTGGGTATAGTTTATGCAATTTTAAGAATTGGTTTAATTGGTTGTGTAGTATGAGCT DDA>@C@DCAEECC>CDCDDDDDHHHHHHEGHEEGGJJIJHGGJIGGGIIHCIGIIJIIGHAIJJIJJJJIGIIIJJJJJIIIJJJIHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:25:12122:5259/1 0 MtDNA 8665 60 101M * 0 0 TTTTTGGTGCTTTGGGTATAGTTTATGCAATTTTAAGAATTGGTTTAATTGGTTGTGTAGTATGAGCTCACCATATGTATACAGTAGGTATAGATTTGGAT FGFAGFDBFD:EEEEEB5AEEEEEAFFFAFDF=FEFEFFFFDAEEFFFFFD:FFACEB:E?:BD?B?EBAFADBBDDBCEBBBAEBEE@EBBE@BFFE?AF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:82:4393:14891/1 0 MtDNA 8674 60 101M * 0 0 CTTTGGGTATAGTTTATGCAATTTTAAGAATTGGTTTAATTGGTTGTGTAGTATGAGCTCACCATATGTATACAGTAGGTATAGATTTGGATTCACGTGCT DGFDDEEAEEEECEEFFEDFGGGGGGEGDFEEEDEDEDBEGEGDGEEEEEFDFFFGGGCBFGGGBFFFDDFDFEFCEEE5@B?D:?CBDB5@B?@CFCF?B PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:3:4300:2958/1 0 MtDNA 8776 60 101M * 0 0 ATTTTTCGGCTGCTACTATAGTTATTGCAGTGCCAACAGGTGTTAAAGTGTTTAGATGATTGGCTACATTATTTGGTATAAAAATGGTATTTAATCCACTT GGGGGGGGGGGGGGGGGGGFGFGGGGGGGDDFFGGEEEEECEEEEECE@EFFFBFAEGBFGGFFFFDEDGCGGGBD=@@D:AA;9EE??=AA@C55>;?BDDCDDBDDBDEDC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:50:14046:18753/1 16 MtDNA 9087 60 101M * 0 0 CTTATGATATCTGCAGTATTTATTTTATTATTTATTGGGGTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATATTT BEEEEEEEEEGEEEEEEEE?D@EGGGEEEEGEEEEEGEGEEGDEGGEEEGEGGFGEGGGGGGEGGGGGDGGGGGEGGEGGGGGGGGGGGGGGGGGGGAGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:10705:30822 99 MtDNA 9102 60 100M = 9126 124 GTATTTATTTTATTATTTATTGGGGTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATATTTAGATTACCCTGATG CBCFFFFFHHHHHJIJJJIJJJJJJCFHIJJJJJGHIJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJIHBEFFFEBBDDDDEEFFEFDDDEDDDDDDDDA PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:72:10661:11179/1 16 MtDNA 9109 60 101M * 0 0 TTTTATTATTTATTGGGGTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATATTTAGATTACCCTGATGTTTATTCG ;EEBEEEEEEEAEEEEEEEEEEEEEEBEDDD@E?EEGEEBEEDEEGDEEDEDCFFCECFFEGGEEGDEEGEFGGGGEFGFGGFGCGGDGGGGGGFGGFFGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:10705:30822 147 MtDNA 9126 60 100M = 9102 -124 GTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATATTTAGATTACCCTGATGTTTATTCGGTATGAAATATTATTG EDDEEDEEEDDDDDDBBDBAADEFFFFFBHHHHHHGJIHIIJJJJJJIIHJJJJJJJJJJJJJJIHG?JJJJJJJJJJJJIJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1314:14393:27976 147 MtDNA 9151 60 100M = 9087 -164 ATTTTGCAGGACTACACGGGTTCCCACGTAAATATTTAGATTACCCTGATGTTTATTCGGTATGAAATATTATTGCCTCTTATGGTTCTATTATTAGAAC CCC@DDCDDEDCCDDBDDDFDBFGFEIJIIGIGJJIJJIHGC=HIIIGFCJJJIIIIGJFIJJIJJJJFIHGJIGDHGIIIGHGIGIHHHHFFFFDD@@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:52:2501:13323/1 0 MtDNA 9191 60 101M * 0 0 TTACCCTGATGTTTATTCGGTATGAAATATTATTGCCTCTTATGGTTCTATTATTAGAACTGCAGGACTATTCTTATTTATTTATGTATTATTAGAGTCTT GGFFGGFGEGGGFGGAFFFFGGGGGGGGEGGGGDGGGGGGGGGGFEGGGGGGGGGGGGGGGGGGGGEGGEGGGGGGGGGEGGGEGGEGGGGGGEFEFCFGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:46:14009:4930/1 0 MtDNA 9196 60 101M * 0 0 CTGATGTTTATTCGGTATGAAATATTATTGCCTCTTATGGTTCTATTATTAGAACTGCAGGACTATTCTTATTTATTTATGTATTATTAGAGTCTTTCTTT EEDBEDEFEFFFFFFEDEEBFFFDFECBE?BEEEEDCCEAEGDG=FEEDFAA:BEB=BBCA=AAAEAFBFFEGGBDF?=FABCCBEAGD=????@EEABE; PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2312:8770:13442 147 MtDNA 9229 60 100M = 9086 -243 CTTATGGTTCTATTATTAGAACTGCAGGACTATTCTTATTTATTTATGTATTATTAGAGTCTTTCTTTAGTTATCGTTTAGTAATTAGAGATTATTATTC CDEEDDEFFFFFFFHHFHHHFJJJJJJIHJJJJJJJJJJJJJJJJJJIIIJJJJJJJJJJJJIJJJJJJJJJJJJIJJJJJJJJJJJHHHHGFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:8985:17141 99 MtDNA 9258 60 100M = 9340 182 CTATTCTTATTTATTTATGTATTATTAGAGTCTTTCTTTAGTTATCGTTTAGTAATTAGAGATTATTATTCTAATAGAAGACCTGAGTATTGTATGAGTA CC@FFFFFHGHHHJJJIJJJGJJIJJIIJJHJJJJJIJJHIHIJIJIHHIHIIJJJJJIHIJJJJJJJGIJJGHGHIDHIIIIIIGIEHDHFHGFFDFDC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:77:4177:11048/1 16 MtDNA 9303 60 101M * 0 0 CGTTTAGTAATTAGAGATTATTATTCTAATAGAAGACCTGAGTATTGTATGAGTAATTATGTATTTGGTCACAGTTATCAGTCTGAGATTTATTTTAGAAC DGEGEEGDEEDGFGEGGEGGGFFGDGGFFFFFFFFAGFDGGFGGDFGGGGGGGGGFGFGGGGGFDGGGGGEGGGGFGGGGGGGGGGGGGGFGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:8985:17141 147 MtDNA 9340 60 100M = 9258 -182 CTGAGTATTGTATGAGTAATTATGTATTTGGTCACAGTTATCAGTCTGAGATTTATTTTAGAACTACTAGATTAAAAAATTAGAAACCTTAGTATAATTT CDDEEEEEFFFFFFFHHHHHHJIHDJJIJJJJIHJJJJJJJJJJJIJJJJJJJJJIJJJJIIIJIHJJJJJJJJJJJJJIIJJJIHJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1305:20758:28262 99 MtDNA 9506 60 100M = 9991 585 GTTCATACCCTTGAGGTGGTTTTCTCTTATTAAAAGTTTTAGTATAAAAAAGTATATTTTATTGTCAATAAAAAGGTAAAAACTTTAGATTCTTTAGTAT CCCFFFFFHHHHHFHJAEHCDHIGJJIJHIJGIHIJFHIJHJGIIJDGHJJGBFHIJJJIGHIGIIIHBDHGGIEACHHFFFFFEEEEEEEDCEDDCCA; PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:76:8573:4029/1 0 MtDNA 9566 60 101M * 0 0 ATTGTCAATAAAAAGGTAAAAACTTTAGATTCTTTAGTATAATTCAGTATGTTTGACTTCCAATCAAAAGGTTTTGAGGATTAATTAATAATTTTTTTCAA DDDDDDD=DDDAB>CD?DDDDDDDDDDBDDDDDDDDDBDDCDDDDDDBDDDBDDDDDADDDDCCDBB=BDDBDDDD?DDBBCCCCCCDDBCBCDBDBCCCB PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:59:16574:9800/1 0 MtDNA 9610 60 101M * 0 0 CAGTATGTTTGACTTCCAATCAAAAGGTTTTGAGGATTAATTAATAATTTTTTTCAAGGATATAATTTACTATTTCAGCATAGTTTATTTGCTAGTTATAT GFAFGFDFFEFFFDDFGGGGGFGCGED=EEDDDDDEGGCGEGGGGGDFGDEFFCE?EGFEGGGDGGFGBGGCGEDGGFG?FDFBFFDGC=AEEAEDDFFDD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:39:12621:19315/1 0 MtDNA 9636 60 101M * 0 0 GTTTTGAGGATTAATTAATAATTTTTTTCAAGGATATAATTTACTATTTCAGCATAGTTTATTTGCTAGTTATATAGATTGATTTCATAGGTTTAATTGTA DBEEEEEEEB?E:EEFFFFB:CDCEFFACFAEEBEBCB?CFEFEFEFFFFFFFDBAC@BC=C;?C:E8FG:GDCGGGFHIIJJEHIIJIJJJJJJIJIJJJJJIFGGIIIJIIIJIIJJIJJIHHGGIFHIIIIHCAEHFHEFFEFFEDEC>@ PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2105:2690:5126 147 MtDNA 10123 60 100M = 10115 -108 TCTGCTGATGTTATTCATGCTTGGGCATTAAATTCTTTATCTGTAAAATTAGATGCTATAAGAGGAATTTTAAGAACATTTAGTTACAGGTTTCCTATAG DEEDEDFFFFFFHHGFGHJGGIJHGDJIJJJJIGJJJIIIJHIIJJJJIGJJJIJJJJJJJIJJJJJIJJIJJJIHJJIJJJJJJJJHHHFBFFFFFCC@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:95:13542:11427/1 0 MtDNA 10154 60 101M * 0 0 ATTCTTTATCTGTAAAATTAGATGCTATAAGAGGAATTTTAAGAACATTTAGTTACAGGTTTCCTATAGTGGGAGTTTTTTATGGTCAATGTTCAGAGATT GGGGDFGFDGEGGGGGFDGGGGGGGGEFGDGGDGFGEGFGFGGGGGGGDGFFEEGEFGBCGGGGGEGFBEEEECEBEEFFGE=EGEECDEEEEGGDCEDDB PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:56:15270:6191/1 16 MtDNA 10392 60 101M * 0 0 ATATAGAGCTTTAAATTTTACTTGTTTAAATATTGGTATTGCATACTATTACAATAAAATTTCATGTTAATGAAAAATAGAAACAAAGGGTAGAGTAAATA GEEEEGEEEEEDBGEFEGEEGGFEGGFGGEEFEGGGEGFGGDEGEGGEGCDFGGGGGGGGGGGGGGFAGGGGGGGGGGGGGGGGFDGGGGGGGEGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:51:14694:9582/1 16 MtDNA 10415 60 101M * 0 0 GTTTAAATATTGGTATTGCATACTATTACAATAAAATTTCATGTTAATGAAAAATAGAAACAAAGGGTAGAGTAAATATTAGTTTTATTGTTTCATACTAA DEDEFEFEDAEEFF?EFFFEEBEAEE=ECCDDFFFFFFFFEFFEFFD=E@DFFFFFFFFFFFDBFFFEAFEFDFFFDEFFFEGGEGGFEGGGGGFFDFDDE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:69:16487:9482/1 0 MtDNA 10798 60 101M * 0 0 TAGGCTTTATATTAAAGCTGGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTT GGGGGGEGGGDEGFGGGGGEGGFGGGGGGGGGGFGDGFGGDGGGEGGFGEGGGGDFEDFBFDEEEEGEFFGCDDFEEGEEEE=GGB@FFDFDGAGAA5EC5 PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:65:19066:9962/1 16 MtDNA 10886 60 101M * 0 0 CTTTTATTGAGTTCCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAATTTAATTTTTATTTAAGAAAAAATAAATATATTTATAC E=@BBD@BEEEE@EE@EEEEEEEBEEBDDDE?FEEBEFFFDDBEBFEEFEDEEEFEBBEAEEFDADFF8CCEEE=??CCCCAFFBFCBEDCB=DEEEEEEE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:57:11714:20002/1 0 MtDNA 11269 60 101M * 0 0 TTTATCTTGATAAATATTTATATTTAATACATTTAGTACGAAAGGAACATTGTAAAAGTTTTAAACTTTAAAGATTTTGAAATCTTTATTTTAGTGCTATT FFFFFEFFFAFFF?FFFFFFFFFFFDFFFFFEFFFFEFFFFDFFBDEFFDFDFFFFFFFFFFFEBFFFFDF=FFFFFFCEEEEFEFFFEFEEE=@BECBBD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:39:7418:8959/1 0 MtDNA 11394 60 101M * 0 0 TTTATTATTTGCTTTTTATTTGATTAATTTTTTATTAAGAATTAAGGATATAGGAAAAAATAAAATTAGAGCGTTTGAATGTGGTTTTGTAAGAGTTGGAA GGGDGG?GGFFGGEGFFDFEFFFAFFFEFFFFFEFFFFFDEFFFDFFFF:E?EEEFACFFFFEEEFFFFAFFBFBFFBFFEBEC=ECEEBBBA=D=?CDAF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:41:12959:4517/1 16 MtDNA 11405 60 101M * 0 0 CTTTTTATTTGATTAATTTTTTATTAAGAATTAAGGATATAGGAAAAAATAAAATTAGAGCGTTTGAATGTGGTTTTGTAAGAGTTGGAAAAATATGATTT EGGEFGGEBGFDGGGGG@GFGGGFEGGGFGGGDGGGGGGDFGGGGGFEGBGFFFD?GFGFEGGGEGGGGEGGGGGGFGDGFGGGGGGGGEGGFGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:5 +EAGLE:2:94:12001:2695/1 0 MtDNA 11494 60 101M * 0 0 AAAATTCAAAATTCTTTTAGAATTCATTTTTTTATTATGATATTGATATTTGTTATTTTTGATTTAGAAATTGTTATGTTTTTAGGTATTTTAGTATCAGA ?EEDEAEEEEDFEFFDEFEDFFEAFDFFFFFFCFDFCFEFF?FFFFFFFAFFDFAFFDFFFFFDFFFDFFFFF=F?DFEFFFA@FF?EFFF?FFEFFF=?: PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:26:8245:3800/1 0 MtDNA 11527 60 101M * 0 0 ATTATGATATTGATATTTGTTATTTTTGATTTAGAAATTGTTATGTTTTTAGGTATTTTAGTATCAGATTTAAGTTCGTATATCAGGTTTTTAATAATATT :FGEEFGFFGGEGGGEFFFFDFFFFGFEGBFFEFEEEEEEDE?EEDEEEBAE?EAEEEECFEDFF=FBFEEA??EEEEEEFEEG?DFF?DEFF?AB;EDDD PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:70:14381:7815/1 0 MtDNA 11624 60 101M * 0 0 TATTCATCTTCATCTTGGGAGGATTTTACATAGAGTGATGATATGGTAAATTAGTTTGAGTAATTTAATTAATATTTCTATTTTTTTGATTGGATTTGTTT GGGGGEGGGGGGFGGEGGGGFGFGGGGAGGGFGGGEGFGFGFEGFGEG?GGGFGFFFFBF@EEDEGEEGBADGFGGGGGGGBGGGDGC@F?EECDFFFDAC PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:41:9505:15324/1 16 MtDNA 11679 60 101M * 0 0 TTGAGTAATTTAATTAATATTTCTATTTTTTTGATTGGATTTGTTTTTTTTATAGGTGGAATTAGTGTTTGGCTTATACCCACATTTAAATTAGGAATCTT EBE=E=EEEEEEEEEE=BEEDEEEEEDEEB?EEEEEEE>EEECCC;EEEEEEEDEEEEE5EDEEEEDDAEEEDDEEDADDD:EEDEDF?AFEBDEDDDBD? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:1:16170:13381/1 0 MtDNA 11707 60 101M * 0 0 TTTTGATTGGATTTGTTTTTTTTATAGGTGGAATTAGTGTTTGGCTTATACCCACATTTAAATTAGGAATCTTTTTTTTAGAATGAGATTTTTTAAGGTTA FFFFFBFFFFFDFDFGEGGGDG>@GAED=EDCCDA:DADCE?EEEFD==FFFEDB?FFFFC:EC:ECCBBC?EEECAFC8=C:=D?E5ECFEFDB:E:CAE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:85:11274:20902/1 0 MtDNA 11790 60 101M * 0 0 TGAGATTTTTTAAGGTTAAAATTTAATTTTTATTTTAATAGAATCTTATTTTCGTTTATTCTTTTTTTGGTAACGTTTAGAGTTTTAGTTTTTAGTAGTTA GGGGFGGGGGGGGGGDGGGFGGGGGFGGFGGGGGGGGGGGGEGGGGFEGGGGGGGGGEGGGGGGGGGGEFCEFFGFFEEEEGEGGGCFDEGFEEF=:6??: PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:1:45:6186:13482/1 0 MtDNA 11795 60 101M * 0 0 TTTTTTAAGGTTAAAATTTAATTTTTATTTTAATAGAATCTTATTTTCGTTTATTCTTTTTTTGGTAACGTTTAGAGTTTTAGTTTTTAGTACTTATTATT DFFFEBAEEEDDADDEDED==CCCCE=EEEEEEDBEEE?EEDDEEAB@EEDDEEDEEAEECEECA>:ACC@DCCB?DAABEBEBCEEEB?EBBEEB=E@EE PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:26:5376:8009/1 0 MtDNA 11861 60 101M * 0 0 AACGTTTAGAGTTTTAGTTTTTAGTACTTATTATTTAAATAGTGAGTTAAACTTTAATTATTATTATTTTGTATTGTTAATTTTCGTAGGTAGAATGTTTA ADCABB PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1301:9999:63927 99 MtDNA 11891 60 100M = 11969 178 TTATTTAAATAGTGAGTTAAACTTTAATTATTATTATTTTGTATTGTTAATTTTCGTAGGTAGAATGTTTAGGCTAAATTTTAGAAACAGTATTTTTACA CCCFFFFFHHHHFHIJJJIJGIJJJJIJJJJJJJJJJJJJJIJJJJIJJJJJJJJJHIJIFGIIJJJIJJJIJIJHGIJJJJJIIHHHHHADFFFFFDCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:18436:71346 99 MtDNA 11949 60 100M = 12056 207 GGTAGAATGTTTAGGCTAAATTTTAGAAACAGTATTTTTACAATGTTACTAAGATGAGATTTATTGGGTATTTCTAGGTTTTTTTTAGTTTTATTTTATA BCBDFFFFHHHHHJJJJJIJJJJJIIIJJJJJCHIIJJJIIIJJJGIIJJJJIIJIGIFHHIHIJIJIFHHJJJJEIJEHIJHFDDDDDDEDDDDEEEDD PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:74:13913:12442/1 16 MtDNA 11959 60 101M * 0 0 TTAGGCTAAATTTTAGAAACAGTATTTTTACAATGTTACTAAGATGAGATTTATTGGGTATTTCTAGGTTTTTTTTAGTTTTATTTTATAATAATTGAGAT GGGEEEFFBEEDGFGGFEDGFGGFGDGGGEGGGGGGGEGGFGFGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGFGGCAAA@D9EEEGGGGGGGGGGGGGGG PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1301:9999:63927 147 MtDNA 11969 60 100M = 11891 -178 TTTTAGAAACAGTATTTTTACAATGTTACTAAGATGAGATTTATTGGGTATTTCTAGGTTTTTTTTAGTTTTATTTTATAATAATTGAGATAGATGTAGG ADEEDDDCCEDDDDDDEEEEDEEEFFFFFFFHHHHHHJJJJJJJJJJJIIJJJIJJJJIIIJJJJJJIJJJJJJJJJJJIIIJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:27:3003:10682/1 16 MtDNA 12043 60 101M * 0 0 TTTATAATAATTGAGATAGATGTAGGGGTGCAATAAATACAGCATTAACTAATCGTCTAGGTGATTATTTTATATTTGTCTTTTTTGGTTTGTCGGTTTTT FB?CEACEE=EDEEE==?EE=DFFEAEEEE;FCEFEBAF?F?FFDFBEDGGFBEGGGGFGGGGEGFF?;FFFFDDDGDEGEFGEGGGGDAFGGGFGDFGGB PG:Z:SNAP RG:Z:266-1 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:2202:18436:71346 147 MtDNA 12056 60 100M = 11949 -207 AGATAGATGTAGGGGTGCAATAAATACAGCATTAACTAATCGTCTAGGTGATTATTTTATATTTGTCTTTTTTGGTTTATCGGTTTTTAGAGGTTATTAT DDEDDDDDDDDDDDDDDDDFEFEDCDDDDDEEDCCEDFFFHHHHJJJJJJJJJJJJJIJJIIJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1116:18352:37917 99 MtDNA 12193 60 100M = 12359 266 TATTATTACTTTTAACAGCTTTTACTAAAAGAGCACAATTTCCATTTAGATCTTGGTTACCCAAAGCTATAAGAGCCCCCACACCGGTGAGGTCTTTGGT @@@FFFDDHGHHHII@FGGGGGGIIIIIIJJIIGGIIJIJFHIGGIJFEHDBGHHGBGIJIHGIAEHIIIJCG@ACCDHEB@,8<995CACA@C>FFFFHGHHHEE@>CDAHFEGIEIJIGIGHIIGIGHGJIIIHIGIHIJGGHDJJIIHEIHHHHHFDFDF@@@ PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:4:6493:13587/1 0 MtDNA 12373 60 101M * 0 0 GTTTTGTTCTGATTATTGGCCTATTTACTATATTTTTTTCTAGCTTAGCAAGTTTGGTCGAAGAAGATTTGAAGAAGGTGGTAGCCTTGAGGACACTTTCA B88:7D??>BGFGDFEDDEFEDBEEEGEEBE=DEEEEEEEEEEGED@EDE5BABBEE>AAA?@?@@?5??BB?BBABA>B?7:??AAABAAAA?A<:>A>? PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:1:5:16925:17939/1 0 MtDNA 12403 60 101M * 0 0 TATTTTTTTCTAGCTTAGCACGTTTGGTCGAAGAAGATTTGAAGAAGGTGGTAGCCTTGAGGACACTTTCACAAATAGGTTTTTCAATAGTTACTTTGGGC FFF5FFEEECGGGEGEDEEE=EEEEEEEEEEEDEEEEEEEEEEEEEEDDEEE=E?BEEEEEEDEEEEEEEEEEEE6CCB>AEEEEEE?BADEEBEEAE=EE PG:Z:SNAP RG:Z:266-1 NM:i:1 +EAGLE:1:79:6338:4546/1 0 MtDNA 12420 60 101M * 0 0 GCAAGTTTGGTCGAAGAAGATTTGAAGAAGGTGGTAGCCTTGAGGACACTTTCACAAATAGGTTTTTCAATAGTTACTTTGGGCCTAGGGCTTAGGTTTAT FFDFFFFFFDEFBFFGGGGGFGEEGGEFFFECEECFFFFFGGGGFGGGGGGGGGFGEFEGGGEGEFFF?DFDFCFEEFEFEBFGEEEEBGEEEEDGEEEEF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1313:2251:84798 99 MtDNA 12449 60 100M = 12482 133 GGTGGTAGCCTTGAGGACACTTTCACAAATAGGTTTTTCAATAGTTACTTTGGGCCTAGGGCTTAGGTTTATTTCATTTATTCATTTAGTTAGACATGCT BCBDFDFFHHHHHJIJIJJJJJJJJJJJIJJIJEFHJJJGIGHJIIJJIJJJIIGHJIIIJJJJJJIEHHIGHHHFHHHFFFFFFFFEEEEEEEDCDDDC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1313:2251:84798 147 MtDNA 12482 60 100M = 12449 -133 TTTTTCAATAGTTACTTTGGGCCTAGGGCTTAGGTTTATTTCATTTATTCATTTAGTTAGACATGCTTTGTTCAAAAGATGTTTATTTATACAAGTAGGT ADDDDDEECEC>CDFFFDHHHHIJJJJJIIJJJJJJJJJJJIJJJJJJJJJJJJIJJIJIIJJJIJJJIIGIJJJJJJJJJJJJJJJHHGHGFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2313:14435:43021 147 MtDNA 12492 60 100M = 12276 -316 GTTACTTTGGGCCTAGGGCTTAGGTTTATTTCATTTAATCATTTAGTTAGACATGCTTTGTTCAAAAGATGTTTATTTATACAAGTAGGTTACATTATTC ACC@CEDCDBDDFFHHHFIGHGIIGIJGIIIJHGHD?4JJJJIHIJJIGIHJIJJJJJIJIIJJJJIJJJJJJJJJJJJIHJJJIJJHHHHHFFFFFC@C PG:Z:SNAP RG:Z:000 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:13602:38947 99 MtDNA 12495 60 100M = 12781 386 ACTTTGGGCCTAGGGCTTAGGTTTATTTCATTTATTCATTTAGTTAGACATGCTTTGTTCAAAAGATGTTTATTTATACAAGTAGGTTACATTATTCATT ?????DD::C:2<3C?;EECFFEIEFIEDE9?@E@DAC;CA7=ACCEDCEIEED>)=?A7?7?DDD@>BD@AD PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:2:102:10210:8668/1 0 MtDNA 12538 60 101M * 0 0 TTAGACATGCTTTGTTCAAAAGATGTTTATTTATACAAGTAGGTTACATTATTCATTGTTCATTTGGACAACAAGATGGACGTAATTATAGGAATAATGGT GFGGGGGGGG:FF?FGGGFGGGFGEBFGGGGFFDDGGGGGGGGAGDEEEEGFFBGGDED;EAGGGDGEEG?FDFFBEG5EEDCEEFDA5FEEBEF?EEB:: PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2214:8352:14578 99 MtDNA 12620 60 100M = 12676 156 TAAGTATAGGAATAATGTTAATTTACCAAATTTTATTCAGTTACAAATATTAGTAACCCTATTTTGTTTATGTGGATTAATTTTCTCAAGGGGTGCTGTA @CC1BDFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJIJJJJJJJJJJJJJJIGHJGIIIGHIFGIJJJJIGHHHFDBABCDDD PG:Z:SNAP RG:Z:000 NM:i:2 +BS-DSFCONTROL03:121:D0M8KACXX:5:2214:8352:14578 147 MtDNA 12676 60 100M = 12620 -156 CCCTATTTTGTTTATGTGGATTAATTTTCTCAAGGGGTGCTGTAAGAAAAGATTTTATTTTAGAATTATTTTTTTCTAATAACTATATAATGTTTTTTAG ?CCDDBDDDDDDDDDDDDDEDDEDEEEAFFFFFHHIIEJJJGIJJJJIHFGJJIJJIIGIJJJJIIJJJIJJJJJJJJJIJJJJJJJHHHHHFFFFFCCC PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:101:7524:6107/1 0 MtDNA 12685 60 101M * 0 0 GTTTATGTGGATTAATTTTCTCAAGGGGTGCTGTAAGAAAAGATTTTATTTTAGAATTATTTTTTTCTAATAACTATATAATGTTTTTTAGATTAATATTT BEEEEDD5DDEEEDEFEDFFAFBDFFFFDFFFFAFEED=E:CBEEFFEDFFEEFEDFFFFDFFFFDF@EFBDFFBEFEFABCD:EFFCCCDD:EADFEFFF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:73:3706:15683/1 0 MtDNA 12685 60 101M * 0 0 GTTTATGTGGATTAATTTTCTCAAGGGGTGCTGTAAGAAAAGATTTTATTTTAGAATTATTTTTTTCTAATAACTATATAATGTTTTTTAGATTAATATTT DDDD:DDBDBBEEEEGFFFGGGFGGGFGDF?ADCEFF=EB?CE:BFF=FCCE>FDEDEAAGAGCCEEGEEGGFEFEFEGD@EFADEFCC>E?E=BBBAEBF PG:Z:SNAP RG:Z:266-1 NM:i:0 +EAGLE:2:35:14999:3587/1 0 MtDNA 12745 60 101M * 0 0 TTTTTTCTAATAACTATATAATGTTTTTTAGATTAATATTTTTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTC DEFFFFFFFFBFD?FFFBFFFFFAFEEBECFDFFFFFDAFFFFFFCFFFBECECEFFEC?FBFFFDDEE??CDBEFFFFEEFFFFABFFFE8CFFFFFFE; PG:Z:SNAP RG:Z:266-1 NM:i:1 +BS-DSFCONTROL03:121:D0M8KACXX:5:1101:18737:28666 99 MtDNA 12755 60 100M = 12783 128 TAACTATATAATGTTTTTTAGATTAATATTTTTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTAAGGTTTAAT CCCFFFFEHHHHHJJJJJJGJGHJJJIGIIJJJJJHIJIIIGHIJJJJJHIIJJJJJIJJJJJIGHHHHFFFFEECECEDDBDDDDDDDDBCCDCCDDDA PG:Z:SNAP RG:Z:000 NM:i:0 +EAGLE:1:53:16100:4061/1 0 MtDNA 12755 60 101M * 0 0 TAACTATATAATGTTTTTTAGATTAATATTTTTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTAAGGTTTAATA FFDFFDEFDFAFE=FDFE@AFB?DFFF5FF?D@<@ACAEEE:ECEEEAA=FFDFDFADDFEEE@EEEEE@AABBB=AEEEDCCFDBEFE@@@DBADEDBBF PG:Z:SNAP RG:Z:266-1 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:1303:13602:38947 147 MtDNA 12781 60 100M = 12495 -386 TATTTTTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTAAGGTTTAATAAAGTAATAAATCATTACAGTAGCAC :?BBBB?CC@5CCCA9'=>>5(;>C;;A>>?;B@BFEFHEBG@=@>FB;BCGB?HEGJHEBEEEFFFGGFFGGE?EEEBD5?BBE PG:Z:SNAP RG:Z:266-1 NM:i:4 +BS-DSFCONTROL03:121:D0M8KACXX:5:1114:11635:22812 99 MtDNA 12940 60 100M = 13083 243 GAATAAATTTTAATCTTCTTAACATCCCAAGACTTTTCTTATACGTAGATTTTTTTGGTCCTCTAGTATTTTTATTTATAATAATTTTTTTATCTTTTTT CCCFFFFFHHHHHJJJJIJJJJJJJIJJJJJJJJJJJJJJJJIIJHIJIJGHJJJJJIHHIJJJFHEHHHHHFFFFFFFEEFEEEFEDDDDDDDDDDDD< PG:Z:SNAP RG:Z:000 NM:i:1 +EAGLE:1:50:13913:15312/1 16 MtDNA 13005 60 101M * 0 0 GTATTTTTATTTATAATAATTTTTTTATCTTTTTTAATTTTAAAAATATTATTTAAGGAGTTAATATACAAGTTTTTAGTTGATTATTTGGCTAAAAATAG ?>:>;::BCBB::FFF@@8<96BABC=@DFFBF=EDFDEFCAE=?EE?FAFEEC?=EEEC=FDEEBEEDAFF?EFFEBEDDEEEEBCDDD?E@DGHGEDBDHHEDHGHIIGCHGI@@FGHIHGGIC==EC>)=A?CD>DDDEEECAACDD PG:Z:SNAP RG:Z:000 NM:i:0 +BS-DSFCONTROL03:121:D0M8KACXX:5:2115:12001:40456 147 MtDNA 13242 60 100M = 13159 -183 GTTTTAATTTTTATTTTTTTTATAATTTGTTAAGGGATTTTAGTTTAATAAAAATATATGTTTTGCATACATAAGATAATAATTCTAGATAGTTTTACAC @DDDDDDDCACDDDFGJIIJIJIIG@HIIJIHIHJJIIGJJIHGGIEIIGIIIIJIGIJJJJIGGCIIIGJIJJJIJJJIHIIJJJJHHFDGFFDDD@@@ PG:Z:SNAP RG:Z:000 NM:i:0 diff -r 000000000000 -r aa82b2e54055 test-data/a.vcf --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/a.vcf Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,192 @@ +##fileformat=VCFv4.2 +##FILTER= +##reference=/home/wolma/galaxy_2017/database/files/000/dataset_35.dat +##contig= +##contig= +##contig= +##contig= +##contig= +##contig= +##contig= +##ALT= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##source=MiModD varcall (version 0.1.8.0) +##MiModDCommand=mimodd varcall /home/wolma/galaxy_2017/database/files/000/dataset_35.dat /home/wolma/galaxy_2017/database/files/000/dataset_54.dat --index-files /home/wolma/galaxy_2017/database/files/_metadata_files/000/metadata_14.dat --ofile /home/wolma/galaxy_2017/database/files/000/dataset_58.dat --depth 250 --verbose --quiet +##samtoolsCommand=n.a. (wrapped by MiModD) +##bcftools_callCommand=n.a. (wrapped by MiModD) +##rginfo= +##rginfo= +##samtoolsVersion=1.2+htslib-1.2.1 +##bcftools_callVersion=1.2+htslib-1.2.1 +##bcftools_concatVersion=1.2+htslib-1.2.1 +##bcftools_viewVersion=1.2+htslib-1.2.1 +##bcftools_viewCommand=view /home/wolma/galaxy_2017/database/files/000/dataset_58.dat +#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT N2 ot266 +chrI 855 . G C 231.0 . DP=27;VDB=2.48853e-13;SGB=13.8822;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,11,13;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:255,72,0:24:0,24:86 +chrI 885 . t tTCT 69.0 . INDEL;IDV=4;IMF=0.0769231;DP=56;VDB=2.27029e-09;SGB=1.27626;MQSB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=16,17,3,1;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,3,60:1:1,0:11 0/1:105,0,255:36:32,4:96 +chrI 912 . A T 195.0 . DP=92;VDB=0.233506;SGB=-8.71232;RPB=0.810719;MQB=1;MQSB=1;BQB=0.974933;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=30,38,9,13;MQ=60 GT:PL:DP:DPR:GQ 0/1:26,0,54:2:1,1:25 0/1:204,0,255:88:67,21:127 +chrI 921 . G A 341.0 . DP=101;VDB=0.0381167;SGB=14.497;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;AC=4;AN=4;DP4=0,1,41,52;MQ=60 GT:PL:DP:DPR:GQ 1/1:110,6,0:2:0,2:25 1/1:255,239,0:92:1,91:127 +chrI 942 . A T 182.0 . DP=147;VDB=0.486211;SGB=-13.0084;RPB=0.177913;MQB=1;MQSB=1;BQB=0.00895523;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=42,63,15,21;MQ=60 GT:PL:DP:DPR:GQ 0/1:20,0,116:4:3,1:17 0/1:198,0,255:137:102,35:127 +chrI 945 . G A 396.0 . DP=144;VDB=0.936097;SGB=17.553;RPB=0.198891;MQB=1;MQSB=1;BQB=0.921548;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=7,15,50,66;MQ=60 GT:PL:DP:DPR:GQ 1/1:177,12,0:4:0,4:19 0/1:255,0,86:134:22,112:78 +chrI 962 . G T 379.0 . DP=141;VDB=0.0554366;SGB=20.8365;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,58,75;MQ=60 GT:PL:DP:DPR:GQ 1/1:148,12,0:4:0,4:36 1/1:255,255,0:129:0,129:127 +chrI 973 . A G,T 329.0 . DP=140;VDB=0.0208629;SGB=18.7462;RPB=0.14071;MQB=1;MQSB=1;BQB=0.978744;MQ0F=0;ICB=0.3;HOB=0.125;AC=3,0;AN=4;DP4=7,12,44,71;MQ=60 GT:PL:DP:DPR:GQ 1/1:110,6,0,110,6,110:2:0,2,0:14 0/1:255,0,65,255,255,255:132:19,112,1:56 +chrI 974 . A T 9.21175 . DP=139;VDB=0.000145084;SGB=10.9211;RPB=0.0841531;MQB=1;MQSB=1;BQB=0.939818;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=44,72,7,11;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:14 0/1:45,0,255:132:114,18:36 +chrI 998 . A T 99.0 . DP=98;VDB=0.00196934;SGB=10.9211;RPB=0.976264;MQB=1;MQSB=1;BQB=0.993458;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=33,40,5,13;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:12 0/1:134,0,255:89:71,18:127 +chrI 1000 . A T 93.0 . DP=108;VDB=0.000325892;SGB=11.6141;RPB=0.945574;MQB=1;MQSB=1;BQB=0.923217;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=34,48,9,10;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:13 0/1:128,0,255:99:80,19:120 +chrI 1028 . G A 105.0 . DP=63;VDB=0.000108954;SGB=6.07412;RPB=0.732968;MQB=1;MQSB=1;BQB=0.91987;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=18,30,6,5;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:12 0/1:140,0,255:57:46,11:127 +chrI 1041 . C T 6.52941 . DP=40;VDB=0.00951951;SGB=1.94933;RPB=0.416648;MQB=1;MQSB=1;BQB=0.652425;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=13,21,3,2;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,71:2:2,0:14 0/1:42,0,255:37:32,5:33 +chrI 1045 . T A 273.0 . DP=36;VDB=1.8168e-09;SGB=2.16465;RPB=0.578971;MQB=1;MQSB=1;BQB=0.637911;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=4,1,11,18;MQ=60 GT:PL:DP:DPR:GQ 1/1:54,6,0:2:0,2:13 0/1:255,0,51:32:5,27:43 +chrI 1048 . G A 266.0 . DP=30;VDB=1.83438e-10;SGB=2.65329;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,10,18;MQ=60 GT:PL:DP:DPR:GQ 1/1:35,3,0:1:0,1:20 1/1:255,81,0:27:0,27:98 +chrI 1053 . c cT 8.64418 . INDEL;IDV=1;IMF=0.047619;DP=21;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:32,3,0:1:0,1:16 +chrI 1054 . A T 231.0 . DP=18;VDB=4.22339e-07;SGB=7.05893;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,5,9;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:255,42,0:14:0,14:54 +chrI 1412 . T C 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrI 1414 . G A 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrI 1421 . G A 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrI 2752 . C T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrI 13851 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13856 . T A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13874 . A G 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13876 . C T 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrI 13901 . C A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13919 . C A 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrI 13921 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 13944 . T A 11.8912 . DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:6 +chrI 13952 . G T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13958 . C T 8.01478 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:32,3,0:1:0,1:4 +chrI 13970 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 13993 . A G 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrI 14201 . G A 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14223 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14242 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14246 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14247 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 22325 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 23943 . g gG 41.4034 . INDEL;IDV=2;IMF=1;DP=2;VDB=0.94;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:66,6,0:2:0,2:9 +chrII 239 . A G 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrII 252 . A T 44.4523 . DP=11;VDB=0.0411132;SGB=0.620439;RPB=0.916482;MQB=0.916482;MQSB=1.00775;BQB=0.366593;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,3,2,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:75,0,160:10:7,3:74 +chrII 265 . A G 161.0 . DP=14;VDB=0.00228484;SGB=4.69269;RPB=0.965069;MQB=1;MQSB=0.966012;BQB=0.686908;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,1,3,6;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:191,0,96:14:5,9:96 +chrII 278 . A T 167.0 . DP=14;VDB=0.000574122;SGB=4.69269;RPB=0.249352;MQB=1;MQSB=0.966012;BQB=0.867428;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=2,3,5,4;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:197,0,111:14:5,9:111 +chrII 279 . A T,G 38.2143 . DP=14;VDB=0.0501588;SGB=1.94933;RPB=0.448332;MQB=1;MQSB=0.966012;BQB=0.118179;MQ0F=0;ICB=1;HOB=0.5;AC=1,0;AN=2;DP4=7,2,0,5;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0,0,0,0:0:0,0,0:0 0/1:69,0,186,71,175,247:14:9,4,1:67 +chrII 291 . A G 86.0 . DP=14;VDB=0.0340507;SGB=1.94933;RPB=1;MQB=1;MQSB=0.966012;BQB=0.197899;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=6,3,1,4;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:116,0,189:14:9,5:116 +chrII 300 . A C 231.0 . DP=14;VDB=3.24567e-05;SGB=7.05893;MQSB=0.966012;MQ0F=0;AC=2;AN=2;DP4=0,0,7,7;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:255,42,0:14:0,14:53 +chrII 304 . A T 137.0 . DP=14;VDB=0.000156924;SGB=4.00342;RPB=0.991701;MQB=1;MQSB=0.966012;BQB=0.703221;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,5,6,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:167,0,107:14:6,8:108 +chrII 305 . A T 27.2811 . DP=14;VDB=0.0785113;SGB=0.620439;RPB=0.973096;MQB=1;MQSB=0.966012;BQB=0.738577;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=6,5,1,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:59,0,236:14:11,3:56 +chrII 317 . A G 101.0 . DP=14;VDB=0.00324086;SGB=2.63044;RPB=0.94925;MQB=1;MQSB=0.966012;BQB=0.997919;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,4,3,3;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:131,0,172:14:8,6:127 +chrII 331 . A T 121.0 . DP=14;VDB=0.0126037;SGB=3.31571;RPB=0.497296;MQB=0.966012;MQSB=0.966012;BQB=0.834543;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=5,2,2,5;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:151,0,154:14:7,7:127 +chrII 343 . A G 90.0 . DP=9;VDB=0.00476095;SGB=1.97062;RPB=0.346719;MQB=0.924584;MQSB=0.924584;BQB=0.462292;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,2,5,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:120,0,74:9:3,6:74 +chrII 11921 . A T 7.18923 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:31,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrII 17381 . G A 4.14838 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:32,0,34:2:1,1:32 ./.:0,0,0:0:0,0:0 +chrII 17518 . A T 14.9987 . DP=3;VDB=0.5;SGB=0.0985265;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,2,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:45,0,29:3:1,2:30 ./.:0,0,0:0:0,0:0 +chrII 19959 . T A 7.18923 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:31,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrIII 7994 . C T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrIII 8004 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrIII 8023 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 8026 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 8027 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 8039 . C A 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrIII 9659 . C T 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrIII 9662 . G T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 9700 . A T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrIV 9450 . A G 4.78389 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:33,0,27:2:1,1:28 ./.:0,0,0:0:0,0:0 +chrIV 16467 . T C 4.14182 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:32,0,25:2:1,1:26 ./.:0,0,0:0:0,0:0 +chrIV 16478 . C G 4.58205 . DP=3;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=0,2,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:35,0,23:2:1,1:28 0/0:0,3,29:1:1,0:5 +chrIV 16499 . A G 3.42278 . DP=3;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=1,1,0,1;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,79:2:2,0:4 0/1:35,3,0:1:0,1:3 +chrIV 16537 . aaga a 25.8002 . INDEL;IDV=1;IMF=1;DP=3;VDB=0.206307;SGB=-0.516033;MQSB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=1,1,0,1;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,120:2:2,0:4 0/1:60,3,0:1:0,1:3 +chrIV 16633 . C A 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrIV 16697 . C T 52.0 . DP=2;VDB=0.48;SGB=-0.759771;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:40,3,0:1:0,1:9 1/1:36,3,0:1:0,1:9 +chrIV 16709 . C G 5.30774 . DP=3;VDB=0.98;SGB=0.0985265;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=0,1,2,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:0,3,39:1:1,0:3 1/1:38,6,0:2:0,2:4 +chrIV 16729 . C T 4.14943 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:0,3,37:1:1,0:3 0/1:35,3,0:1:0,1:3 +chrIV 16769 . A T 13.7861 . DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:6 +chrIV 16814 . A G 3.03341 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:30,0,31:2:1,1:30 +chrIV 16815 . C A 48.4033 . DP=2;VDB=0.42;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:73,6,0:2:0,2:9 +chrIV 16823 . A C 4.14995 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,28:2:1,1:29 +chrIV 16829 . T C 3.56356 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:31,0,32:2:1,1:31 +chrIV 16833 . A T,G 14.5409 . DP=2;VDB=0.42;SGB=0.0985265;MQSB=1;MQ0F=0;AC=1,1;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0,0,0,0:0:0,0,0:0 1/2:65,34,31,34,0,31:2:0,1,1:29 +chrIV 16834 . T A 3.56452 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:31,0,27:2:1,1:28 +chrIV 16894 . T C 55.0 . DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:9 1/1:38,3,0:1:0,1:9 +chrIV 16899 . T A 49.5769 . DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:9 1/1:33,3,0:1:0,1:9 +chrIV 16902 . T G 55.0 . DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:9 1/1:38,3,0:1:0,1:9 +chrIV 16921 . A G 68.0 . DP=3;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:54,3,0:1:0,1:11 1/1:38,3,0:1:0,1:11 +chrIV 16951 . C A 76.0 . DP=3;VDB=0.740702;SGB=-0.8159;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,2,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:35,3,0:1:0,1:11 1/1:65,6,0:2:0,2:13 +chrIV 17000 . T C 149.0 . DP=7;VDB=0.103091;SGB=-0.680382;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,6,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:14 1/1:132,18,0:6:0,6:29 +chrIV 17020 . C G,A 99.0 . DP=8;VDB=0.391417;SGB=2.55596;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0;AC=1,1;AN=4;DP4=0,1,7,0;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,3,34,3,34,34:1:1,0,0:3 1/2:150,33,15,125,0,122:7:0,6,1:10 +chrIV 17024 . T C 14.4249 . DP=7;VDB=0.48;SGB=0.00473136;RPB=0.6;MQB=1;BQB=0.8;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=5,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:45,0,103:7:5,2:44 +chrIV 17037 . T A 37.8983 . DP=7;VDB=0.460446;SGB=0.620439;RPB=1.01283;MQB=1.01283;BQB=0.810265;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,0,3,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:68,0,95:7:4,3:69 +chrIV 17051 . C T 103.0 . DP=6;VDB=0.534935;SGB=1.41704;MQ0F=0;AC=2;AN=2;DP4=0,0,5,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:127,15,0:5:0,5:22 +chrIV 17112 . T A 3.03494 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:30,0,30:2:1,1:30 +chrV 5001 . T G 4.94666 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:28,3,0:1:0,1:4 +chrV 10045 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrV 10095 . T A 50.0 . DP=2;VDB=0.56;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:75,6,0:2:0,2:9 +chrV 10108 . C A 4.15167 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,32:2:1,1:32 +chrV 10158 . C T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrV 10166 . G A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10205 . A G 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10229 . G C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10249 . G T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10250 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrV 10251 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10257 . A T 6.39934 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:30,3,0:1:0,1:4 +chrV 10279 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 14271 . T C 39.4036 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:64,6,0:2:0,2:9 +chrV 14273 . G C 39.4036 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:64,6,0:2:0,2:9 +chrV 14285 . T C 76.0 . DP=3;VDB=0.074936;SGB=0.461295;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,2,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:100,9,0:3:0,3:13 +chrV 14335 . T G 19.5632 . DP=6;VDB=0.18;SGB=0.00473136;RPB=1;MQB=1;MQSB=1;BQB=0.875;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,0,0,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:50,0,98:6:4,2:49 +chrV 14337 . A G 100.0 . DP=6;VDB=0.405004;SGB=1.41704;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,4,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:132,0,14:6:1,5:9 +chrV 14339 . A G 154.0 . DP=6;VDB=0.189016;SGB=1.97062;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,4,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:178,18,0:6:0,6:25 +chrV 14346 . C T 100.0 . DP=6;VDB=0.405004;SGB=1.41704;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,4,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:132,0,18:6:1,5:14 +chrV 14367 . C A 104.0 . DP=5;VDB=0.220638;SGB=0.908071;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,2,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:128,12,0:4:0,4:19 +chrV 14445 . A G 21.4343 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,0,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:46,6,0:2:0,2:9 +chrV 14481 . G A 4.15167 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,32:2:1,1:32 +chrV 14488 . G T 4.15167 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,32:2:1,1:32 +chrV 14528 . G A 3.56356 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:31,0,32:2:1,1:31 +chrV 14530 . A G 4.13076 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,22:2:1,1:23 +chrV 14538 . T C 32.4055 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,0,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:57,6,0:2:0,2:9 +chrX 1592 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 1622 . G T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 1648 . A C 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrX 1650 . T A 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrX 6015 . A T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:38,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6032 . G A 8.01478 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:32,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6058 . ta t 15.3937 . INDEL;IDV=1;IMF=1;DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:40,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6118 . A G 15.394 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:40,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6143 . G A 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:37,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6984 . T A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 7025 . A G 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrX 10527 . c cC 8.86989 . INDEL;IDV=1;IMF=1;DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:33,3,0:1:0,1:4 +chrX 10535 . T C 11.8912 . DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:6 +chrX 10562 . C G 18.2649 . DP=7;VDB=0.18;SGB=0.00473136;RPB=0.8;MQB=1;MQSB=1;BQB=0.9;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,1,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:49,0,131:7:5,2:48 +chrX 10595 . T G 148.0 . DP=7;VDB=0.00429645;SGB=2.55596;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,6,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:172,21,0:7:0,7:29 +chrX 10629 . gt g 45.8065 . INDEL;IDV=4;IMF=0.666667;DP=6;VDB=0.14;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:70,6,0:2:0,2:13 +chrX 10630 . T C,G 10.4616 . DP=2;VDB=0.32;SGB=0.0985265;MQSB=1;MQ0F=0;AC=1,1;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0,0,0,0:0:0,0,0:0 1/2:58,29,26,32,0,29:2:0,1,1:25 +chrX 10643 . G T 34.5919 . DP=3;VDB=0.02;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:59,6,0:2:0,2:11 +chrX 15480 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 15495 . G C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 24890 . C G 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 24935 . A C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +MtDNA 954 . G T 4.79534 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ 0/1:36,3,0:1:0,1:3 0/1:0,3,33:1:1,0:3 +MtDNA 5979 . C A 7.18923 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:31,3,0:1:0,1:4 +MtDNA 8429 . A G 38.5774 . DP=2;VDB=0.36;SGB=-0.759771;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:26,3,0:1:0,1:9 1/1:37,3,0:1:0,1:9 +MtDNA 12134 . A G 6.22224 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ 0/1:0,3,41:1:1,0:3 0/1:38,3,0:1:0,1:3 +MtDNA 12637 . G T 8.72328 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=1,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:41,3,0:1:0,1:3 0/1:0,3,25:1:1,0:3 +MtDNA 12998 . C T 14.4264 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:39,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 diff -r 000000000000 -r aa82b2e54055 test-data/a_part1.bam Binary file test-data/a_part1.bam has changed diff -r 000000000000 -r aa82b2e54055 test-data/a_part2.bam Binary file test-data/a_part2.bam has changed diff -r 000000000000 -r aa82b2e54055 test-data/a_part2.bcf Binary file test-data/a_part2.bcf has changed diff -r 000000000000 -r aa82b2e54055 test-data/ce11ToCe10.over.chain --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/ce11ToCe10.over.chain Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,871 @@ +chain 1420037770 chrI 15072434 + 0 15072434 chrI 15072423 + 0 15072423 5 +288367 0 1 +49582 0 1 +3348 0 1 +884584 1 0 +731267 0 1 +203378 1 0 +1324625 0 1 +399852 13 13 +122370 1 0 +213502 1 0 +782497 1 0 +39234 0 3 +867656 1 0 +595979 1 0 +295566 2 0 +147478 1 1 +23 1 1 +191487 13 13 +8983 1 0 +213892 1 0 +58238 1 0 +125173 11 11 +206247 1 0 +126100 1 1 +46 1 1 +281857 1 0 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test-data/reads_1_w_header.bam has changed diff -r 000000000000 -r aa82b2e54055 test-data/reads_1and2_w_header.bam Binary file test-data/reads_1and2_w_header.bam has changed diff -r 000000000000 -r aa82b2e54055 test-data/rebased.vcf --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/rebased.vcf Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,186 @@ +##fileformat=VCFv4.2 +##FILTER= +##reference=/home/wolma/galaxy_2017/database/files/000/dataset_35.dat +##contig= +##contig= +##contig= +##contig= +##contig= +##contig= +##contig= +##ALT= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##FORMAT= +##source=MiModD varcall (version 0.1.8.0) +##MiModDCommand=mimodd varcall /home/wolma/galaxy_2017/database/files/000/dataset_35.dat /home/wolma/galaxy_2017/database/files/000/dataset_54.dat --index-files /home/wolma/galaxy_2017/database/files/_metadata_files/000/metadata_14.dat --ofile /home/wolma/galaxy_2017/database/files/000/dataset_58.dat --depth 250 --verbose --quiet +##samtoolsCommand=n.a. (wrapped by MiModD) +##bcftools_callCommand=n.a. (wrapped by MiModD) +##rginfo= +##rginfo= +##samtoolsVersion=1.2+htslib-1.2.1 +##bcftools_callVersion=1.2+htslib-1.2.1 +##bcftools_concatVersion=1.2+htslib-1.2.1 +##bcftools_viewVersion=1.2+htslib-1.2.1 +##bcftools_viewCommand=view /home/wolma/galaxy_2017/database/files/000/dataset_58.dat +#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT N2 ot266 +chrI 855 . G C 231.0 . DP=27;VDB=2.48853e-13;SGB=13.8822;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,11,13;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:255,72,0:24:0,24:86 +chrI 885 . t tTCT 69.0 . INDEL;IDV=4;IMF=0.0769231;DP=56;VDB=2.27029e-09;SGB=1.27626;MQSB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=16,17,3,1;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,3,60:1:1,0:11 0/1:105,0,255:36:32,4:96 +chrI 912 . A T 195.0 . DP=92;VDB=0.233506;SGB=-8.71232;RPB=0.810719;MQB=1;MQSB=1;BQB=0.974933;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=30,38,9,13;MQ=60 GT:PL:DP:DPR:GQ 0/1:26,0,54:2:1,1:25 0/1:204,0,255:88:67,21:127 +chrI 921 . G A 341.0 . DP=101;VDB=0.0381167;SGB=14.497;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;AC=4;AN=4;DP4=0,1,41,52;MQ=60 GT:PL:DP:DPR:GQ 1/1:110,6,0:2:0,2:25 1/1:255,239,0:92:1,91:127 +chrI 942 . A T 182.0 . DP=147;VDB=0.486211;SGB=-13.0084;RPB=0.177913;MQB=1;MQSB=1;BQB=0.00895523;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=42,63,15,21;MQ=60 GT:PL:DP:DPR:GQ 0/1:20,0,116:4:3,1:17 0/1:198,0,255:137:102,35:127 +chrI 945 . G A 396.0 . DP=144;VDB=0.936097;SGB=17.553;RPB=0.198891;MQB=1;MQSB=1;BQB=0.921548;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=7,15,50,66;MQ=60 GT:PL:DP:DPR:GQ 1/1:177,12,0:4:0,4:19 0/1:255,0,86:134:22,112:78 +chrI 962 . G T 379.0 . DP=141;VDB=0.0554366;SGB=20.8365;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,58,75;MQ=60 GT:PL:DP:DPR:GQ 1/1:148,12,0:4:0,4:36 1/1:255,255,0:129:0,129:127 +chrI 973 . A G,T 329.0 . DP=140;VDB=0.0208629;SGB=18.7462;RPB=0.14071;MQB=1;MQSB=1;BQB=0.978744;MQ0F=0;ICB=0.3;HOB=0.125;AC=3,0;AN=4;DP4=7,12,44,71;MQ=60 GT:PL:DP:DPR:GQ 1/1:110,6,0,110,6,110:2:0,2,0:14 0/1:255,0,65,255,255,255:132:19,112,1:56 +chrI 974 . A T 9.21175 . DP=139;VDB=0.000145084;SGB=10.9211;RPB=0.0841531;MQB=1;MQSB=1;BQB=0.939818;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=44,72,7,11;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:14 0/1:45,0,255:132:114,18:36 +chrI 998 . A T 99.0 . DP=98;VDB=0.00196934;SGB=10.9211;RPB=0.976264;MQB=1;MQSB=1;BQB=0.993458;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=33,40,5,13;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:12 0/1:134,0,255:89:71,18:127 +chrI 1000 . A T 93.0 . DP=108;VDB=0.000325892;SGB=11.6141;RPB=0.945574;MQB=1;MQSB=1;BQB=0.923217;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=34,48,9,10;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:13 0/1:128,0,255:99:80,19:120 +chrI 1028 . G A 105.0 . DP=63;VDB=0.000108954;SGB=6.07412;RPB=0.732968;MQB=1;MQSB=1;BQB=0.91987;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=18,30,6,5;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,110:2:2,0:12 0/1:140,0,255:57:46,11:127 +chrI 1041 . C T 6.52941 . DP=40;VDB=0.00951951;SGB=1.94933;RPB=0.416648;MQB=1;MQSB=1;BQB=0.652425;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=13,21,3,2;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,71:2:2,0:14 0/1:42,0,255:37:32,5:33 +chrI 1045 . T A 273.0 . DP=36;VDB=1.8168e-09;SGB=2.16465;RPB=0.578971;MQB=1;MQSB=1;BQB=0.637911;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=4,1,11,18;MQ=60 GT:PL:DP:DPR:GQ 1/1:54,6,0:2:0,2:13 0/1:255,0,51:32:5,27:43 +chrI 1048 . G A 266.0 . DP=30;VDB=1.83438e-10;SGB=2.65329;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,10,18;MQ=60 GT:PL:DP:DPR:GQ 1/1:35,3,0:1:0,1:20 1/1:255,81,0:27:0,27:98 +chrI 1053 . c cT 8.64418 . INDEL;IDV=1;IMF=0.047619;DP=21;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:32,3,0:1:0,1:16 +chrI 1054 . A T 231.0 . DP=18;VDB=4.22339e-07;SGB=7.05893;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,5,9;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:255,42,0:14:0,14:54 +chrI 1412 . T C 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrI 1414 . G A 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrI 1421 . G A 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrI 2752 . C T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrI 13851 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13856 . T A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13874 . A G 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13876 . C T 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrI 13901 . C A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13919 . C A 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrI 13921 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 13944 . T A 11.8912 . DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:6 +chrI 13952 . G T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrI 13958 . C T 8.01478 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:32,3,0:1:0,1:4 +chrI 13970 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 13993 . A G 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrI 14201 . G A 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14223 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14242 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14246 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 14247 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 22325 . T C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrI 23943 . g gG 41.4034 . INDEL;IDV=2;IMF=1;DP=2;VDB=0.94;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:66,6,0:2:0,2:9 +chrII 239 . A G 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrII 252 . A T 44.4523 . DP=11;VDB=0.0411132;SGB=0.620439;RPB=0.916482;MQB=0.916482;MQSB=1.00775;BQB=0.366593;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,3,2,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:75,0,160:10:7,3:74 +chrII 265 . A G 161.0 . DP=14;VDB=0.00228484;SGB=4.69269;RPB=0.965069;MQB=1;MQSB=0.966012;BQB=0.686908;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,1,3,6;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:191,0,96:14:5,9:96 +chrII 278 . A T 167.0 . DP=14;VDB=0.000574122;SGB=4.69269;RPB=0.249352;MQB=1;MQSB=0.966012;BQB=0.867428;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=2,3,5,4;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:197,0,111:14:5,9:111 +chrII 279 . A T,G 38.2143 . DP=14;VDB=0.0501588;SGB=1.94933;RPB=0.448332;MQB=1;MQSB=0.966012;BQB=0.118179;MQ0F=0;ICB=1;HOB=0.5;AC=1,0;AN=2;DP4=7,2,0,5;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0,0,0,0:0:0,0,0:0 0/1:69,0,186,71,175,247:14:9,4,1:67 +chrII 291 . A G 86.0 . DP=14;VDB=0.0340507;SGB=1.94933;RPB=1;MQB=1;MQSB=0.966012;BQB=0.197899;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=6,3,1,4;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:116,0,189:14:9,5:116 +chrII 300 . A C 231.0 . DP=14;VDB=3.24567e-05;SGB=7.05893;MQSB=0.966012;MQ0F=0;AC=2;AN=2;DP4=0,0,7,7;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:255,42,0:14:0,14:53 +chrII 304 . A T 137.0 . DP=14;VDB=0.000156924;SGB=4.00342;RPB=0.991701;MQB=1;MQSB=0.966012;BQB=0.703221;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,5,6,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:167,0,107:14:6,8:108 +chrII 305 . A T 27.2811 . DP=14;VDB=0.0785113;SGB=0.620439;RPB=0.973096;MQB=1;MQSB=0.966012;BQB=0.738577;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=6,5,1,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:59,0,236:14:11,3:56 +chrII 317 . A G 101.0 . DP=14;VDB=0.00324086;SGB=2.63044;RPB=0.94925;MQB=1;MQSB=0.966012;BQB=0.997919;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,4,3,3;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:131,0,172:14:8,6:127 +chrII 331 . A T 121.0 . DP=14;VDB=0.0126037;SGB=3.31571;RPB=0.497296;MQB=0.966012;MQSB=0.966012;BQB=0.834543;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=5,2,2,5;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:151,0,154:14:7,7:127 +chrII 343 . A G 90.0 . DP=9;VDB=0.00476095;SGB=1.97062;RPB=0.346719;MQB=0.924584;MQSB=0.924584;BQB=0.462292;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,2,5,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:120,0,74:9:3,6:74 +chrII 11921 . A T 7.18923 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:31,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrII 17381 . G A 4.14838 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:32,0,34:2:1,1:32 ./.:0,0,0:0:0,0:0 +chrII 17518 . A T 14.9987 . DP=3;VDB=0.5;SGB=0.0985265;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,2,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:45,0,29:3:1,2:30 ./.:0,0,0:0:0,0:0 +chrII 19959 . T A 7.18923 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:31,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrIII 7994 . C T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrIII 8004 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrIII 8023 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 8026 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 8027 . A G 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 8039 . C A 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrIII 9659 . C T 9.75336 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:34,3,0:1:0,1:4 +chrIII 9662 . G T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrIII 9700 . A T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrIV 9450 . A G 4.78389 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:33,0,27:2:1,1:28 ./.:0,0,0:0:0,0:0 +chrIV 16467 . T C 4.14182 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:32,0,25:2:1,1:26 ./.:0,0,0:0:0,0:0 +chrIV 16478 . C G 4.58205 . DP=3;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=0,2,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:35,0,23:2:1,1:28 0/0:0,3,29:1:1,0:5 +chrIV 16499 . A G 3.42278 . DP=3;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=1,1,0,1;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,79:2:2,0:4 0/1:35,3,0:1:0,1:3 +chrIV 16537 . aaga a 25.8002 . INDEL;IDV=1;IMF=1;DP=3;VDB=0.206307;SGB=-0.516033;MQSB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=1,1,0,1;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,6,120:2:2,0:4 0/1:60,3,0:1:0,1:3 +chrIV 16633 . C A 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrIV 16697 . C T 52.0 . DP=2;VDB=0.48;SGB=-0.759771;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:40,3,0:1:0,1:9 1/1:36,3,0:1:0,1:9 +chrIV 16709 . C G 5.30774 . DP=3;VDB=0.98;SGB=0.0985265;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=0,1,2,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:0,3,39:1:1,0:3 1/1:38,6,0:2:0,2:4 +chrIV 16729 . C T 4.14943 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ 0/1:0,3,37:1:1,0:3 0/1:35,3,0:1:0,1:3 +chrIV 16769 . A T 13.7861 . DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:6 +chrIV 16814 . A G 3.03341 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:30,0,31:2:1,1:30 +chrIV 16815 . C A 48.4033 . DP=2;VDB=0.42;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:73,6,0:2:0,2:9 +chrIV 16823 . A C 4.14995 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,28:2:1,1:29 +chrIV 16829 . T C 3.56356 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:31,0,32:2:1,1:31 +chrIV 16833 . A T,G 14.5409 . DP=2;VDB=0.42;SGB=0.0985265;MQSB=1;MQ0F=0;AC=1,1;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0,0,0,0:0:0,0,0:0 1/2:65,34,31,34,0,31:2:0,1,1:29 +chrIV 16834 . T A 3.56452 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:31,0,27:2:1,1:28 +chrIV 16894 . T C 55.0 . DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:9 1/1:38,3,0:1:0,1:9 +chrIV 16899 . T A 49.5769 . DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:9 1/1:33,3,0:1:0,1:9 +chrIV 16902 . T G 55.0 . DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:9 1/1:38,3,0:1:0,1:9 +chrIV 16921 . A G 68.0 . DP=3;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:54,3,0:1:0,1:11 1/1:38,3,0:1:0,1:11 +chrIV 16951 . C A 76.0 . DP=3;VDB=0.740702;SGB=-0.8159;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,2,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:35,3,0:1:0,1:11 1/1:65,6,0:2:0,2:13 +chrIV 17000 . T C 149.0 . DP=7;VDB=0.103091;SGB=-0.680382;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,6,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:41,3,0:1:0,1:14 1/1:132,18,0:6:0,6:29 +chrIV 17020 . C G,A 99.0 . DP=8;VDB=0.391417;SGB=2.55596;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0;AC=1,1;AN=4;DP4=0,1,7,0;MQ=60 GT:PL:DP:DPR:GQ 0/0:0,3,34,3,34,34:1:1,0,0:3 1/2:150,33,15,125,0,122:7:0,6,1:10 +chrIV 17024 . T C 14.4249 . DP=7;VDB=0.48;SGB=0.00473136;RPB=0.6;MQB=1;BQB=0.8;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=5,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:45,0,103:7:5,2:44 +chrIV 17037 . T A 37.8983 . DP=7;VDB=0.460446;SGB=0.620439;RPB=1.01283;MQB=1.01283;BQB=0.810265;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,0,3,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:68,0,95:7:4,3:69 +chrIV 17051 . C T 103.0 . DP=6;VDB=0.534935;SGB=1.41704;MQ0F=0;AC=2;AN=2;DP4=0,0,5,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:127,15,0:5:0,5:22 +chrIV 17112 . T A 3.03494 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:30,0,30:2:1,1:30 +chrV 5001 . T G 4.94666 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:28,3,0:1:0,1:4 +chrV 10045 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrV 10095 . T A 50.0 . DP=2;VDB=0.56;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:75,6,0:2:0,2:9 +chrV 10108 . C A 4.15167 . DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,32:2:1,1:32 +chrV 10158 . C T 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrV 10166 . G A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10205 . A G 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10229 . G C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10249 . G T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10250 . C T 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 +chrV 10251 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 10257 . A T 6.39934 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:30,3,0:1:0,1:4 +chrV 10279 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrV 14271 . T C 39.4036 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:64,6,0:2:0,2:9 +chrV 14273 . G C 39.4036 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:64,6,0:2:0,2:9 +chrV 14285 . T C 76.0 . DP=3;VDB=0.074936;SGB=0.461295;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,2,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:100,9,0:3:0,3:13 +chrV 14335 . T G 19.5632 . DP=6;VDB=0.18;SGB=0.00473136;RPB=1;MQB=1;MQSB=1;BQB=0.875;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,0,0,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:50,0,98:6:4,2:49 +chrV 14337 . A G 100.0 . DP=6;VDB=0.405004;SGB=1.41704;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,4,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:132,0,14:6:1,5:9 +chrV 14339 . A G 154.0 . DP=6;VDB=0.189016;SGB=1.97062;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,4,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:178,18,0:6:0,6:25 +chrV 14346 . C T 100.0 . DP=6;VDB=0.405004;SGB=1.41704;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,4,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:132,0,18:6:1,5:14 +chrV 14367 . C A 104.0 . DP=5;VDB=0.220638;SGB=0.908071;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,2,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:128,12,0:4:0,4:19 +chrV 14445 . A G 21.4343 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,0,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:46,6,0:2:0,2:9 +chrV 14481 . G A 4.15167 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,32:2:1,1:32 +chrV 14488 . G T 4.15167 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,32:2:1,1:32 +chrV 14528 . G A 3.56356 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:31,0,32:2:1,1:31 +chrV 14530 . A G 4.13076 . DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:32,0,22:2:1,1:23 +chrV 14538 . T C 32.4055 . DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,0,2;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:57,6,0:2:0,2:9 +chrX 1592 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 1622 . G T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 1648 . A C 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrX 1650 . T A 10.6576 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:35,3,0:1:0,1:4 +chrX 6015 . A T 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:38,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6032 . G A 8.01478 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ 1/1:32,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6058 . ta t 15.3937 . INDEL;IDV=1;IMF=1;DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:40,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6118 . A G 15.394 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:40,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6143 . G A 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60 GT:PL:DP:DPR:GQ 1/1:37,3,0:1:0,1:4 ./.:0,0,0:0:0,0:0 +chrX 6984 . T A 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 7025 . A G 12.5173 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:37,3,0:1:0,1:4 +chrX 10527 . c cC 8.86989 . INDEL;IDV=1;IMF=1;DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:33,3,0:1:0,1:4 +chrX 10535 . T C 11.8912 . DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:6 +chrX 10562 . C G 18.2649 . DP=7;VDB=0.18;SGB=0.00473136;RPB=0.8;MQB=1;MQSB=1;BQB=0.9;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,1,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 0/1:49,0,131:7:5,2:48 +chrX 10595 . T G 148.0 . DP=7;VDB=0.00429645;SGB=2.55596;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,6,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:172,21,0:7:0,7:29 +chrX 10629 . gt g 45.8065 . INDEL;IDV=4;IMF=0.666667;DP=6;VDB=0.14;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:70,6,0:2:0,2:13 +chrX 10630 . T C,G 10.4616 . DP=2;VDB=0.32;SGB=0.0985265;MQSB=1;MQ0F=0;AC=1,1;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0,0,0,0:0:0,0,0:0 1/2:58,29,26,32,0,29:2:0,1,1:25 +chrX 10643 . G T 34.5919 . DP=3;VDB=0.02;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:59,6,0:2:0,2:11 +chrX 15480 . T C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 15495 . G C 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 24890 . C G 13.4669 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:38,3,0:1:0,1:4 +chrX 24935 . A C 11.5799 . DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60 GT:PL:DP:DPR:GQ ./.:0,0,0:0:0,0:0 1/1:36,3,0:1:0,1:4 diff -r 000000000000 -r aa82b2e54055 test-data/so_coordinate.bam Binary file test-data/so_coordinate.bam has changed diff -r 000000000000 -r aa82b2e54055 test-data/so_queryname.sam --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/so_queryname.sam Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,2 @@ +@HD VN:1.5 SO:queryname +@RG ID:000 SM:N2 DS:C. elegans wt CN:ABC DT:2017-06-27 LB:XYZ PI:400 PL:ILLUMINA PU:SEQ123 diff -r 000000000000 -r aa82b2e54055 test-data/split_pair_reads_1.fastqsanger --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_pair_reads_1.fastqsanger Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,20 @@ +@HWI-EAS91_1_30788AAXX:7:21:1542:1758/1 +GTCAATTGTACTGGTCAATACTAAAAGAATAGGATC ++HWI-EAS91_1_30788AAXX:7:21:1542:1758/1 +hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh +@HWI-EAS91_1_30788AAXX:7:22:1621:462/1 +ATAATGGCTATTATTGTGGGGGGGATGATGCTGGAA ++HWI-EAS91_1_30788AAXX:7:22:1621:462/1 +hhhhhhhhhhhhQAhh@hhhhNhhhfhMbCIScC?h +@HWI-EAS91_1_30788AAXX:7:45:408:807/1 +TACCCGATTTTTTGCTTTCCACTTTATCCTACCCTT ++HWI-EAS91_1_30788AAXX:7:45:408:807/1 +hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh +@HWI-EAS91_1_30788AAXX:7:49:654:1439/1 +CTAACTCTATTTATTGTATTTCAACTAAAAATCTCA ++HWI-EAS91_1_30788AAXX:7:49:654:1439/1 +hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh +@HWI-EAS91_1_30788AAXX:7:64:947:234/1 +TATCAAAAAAGAATATAATCTGAATCAACACTACAA ++HWI-EAS91_1_30788AAXX:7:64:947:234/1 +hhhhhhhhhhhhhhhhhhhhhhhRhhehhahhhhhJ diff -r 000000000000 -r aa82b2e54055 test-data/split_pair_reads_2.fastqsanger --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/split_pair_reads_2.fastqsanger Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,20 @@ +@HWI-EAS91_1_30788AAXX:7:21:1542:1758/2 +GCTCCTAGCATCTGGAGTCTCTATCACCTGAGCCCA ++HWI-EAS91_1_30788AAXX:7:21:1542:1758/2 +hhhhhhhhhhhhhhhhhhhhhhhh`hfhhVZSWehR +@HWI-EAS91_1_30788AAXX:7:22:1621:462/2 +ACTAGCCCCAATATCAATCCTATATCAAATCTCACC ++HWI-EAS91_1_30788AAXX:7:22:1621:462/2 +hJhhhhChhhJhhhRhhKhePhc\KhhV\KhXhJhh +@HWI-EAS91_1_30788AAXX:7:45:408:807/2 +ATGAGTGCTAGGATCAGGATGGAGAGGATTAGGGCT ++HWI-EAS91_1_30788AAXX:7:45:408:807/2 +hhhhhhhhhhhhhhhhhh`hhhZh`hhhhhRXhhYh +@HWI-EAS91_1_30788AAXX:7:49:654:1439/2 +TAGGTTTATTGATAGTTGTGTTGTTGGTGTAAATGG ++HWI-EAS91_1_30788AAXX:7:49:654:1439/2 +hhhhhhhhhhhhhhhhhhhhhhhhhdhh_hG\XhU@ +@HWI-EAS91_1_30788AAXX:7:64:947:234/2 +CCTATTAGTGTGTAGAATAGGAAGTAGAGGCCTGCG ++HWI-EAS91_1_30788AAXX:7:64:947:234/2 +hhhhhhhh^hPhWfhhhhThWUhhfhh_hhNIVPUd diff -r 000000000000 -r aa82b2e54055 test-data/vaf_linkage.pdf Binary file test-data/vaf_linkage.pdf has changed diff -r 000000000000 -r aa82b2e54055 tool-data/all_fasta.loc.sample --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool-data/all_fasta.loc.sample Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,18 @@ +#This file lists the locations and dbkeys of all the fasta files +#under the "genome" directory (a directory that contains a directory +#for each build). The script extract_fasta.py will generate the file +#all_fasta.loc. This file has the format (white space characters are +#TAB characters): +# +# +# +#So, all_fasta.loc could look something like this: +# +#apiMel3 apiMel3 Honeybee (Apis mellifera): apiMel3 /path/to/genome/apiMel3/apiMel3.fa +#hg19canon hg19 Human (Homo sapiens): hg19 Canonical /path/to/genome/hg19/hg19canon.fa +#hg19full hg19 Human (Homo sapiens): hg19 Full /path/to/genome/hg19/hg19full.fa +# +#Your all_fasta.loc file should contain an entry for each individual +#fasta file. So there will be multiple fasta files for each build, +#such as with hg19 above. +# diff -r 000000000000 -r aa82b2e54055 tool_data_table_conf.xml.sample --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_data_table_conf.xml.sample Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,7 @@ + + + + value, dbkey, name, path + +
+
diff -r 000000000000 -r aa82b2e54055 varextract.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/varextract.xml Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,184 @@ + + from a BCF file + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r aa82b2e54055 varreport.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/varreport.xml Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,140 @@ + + + in a human-friendly format that simplifies data exploration + + + macros.xml + + + + +

MiModD has generated the variant report you requested, but for technical reasons, we have to take you through this little detour to display the report in Galaxy.

Proceed to the variant report.

' + > '$ofile' + #else + --ofile '$ofile' + #end if + ]]> + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff -r 000000000000 -r aa82b2e54055 vcf_filter.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/vcf_filter.xml Sat Nov 11 18:19:22 2017 -0500 @@ -0,0 +1,191 @@ + + + extracts lines from a vcf variant file based on field-specific filters + + + macros.xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + keep all variants in the vcf input file for which the specified sample's genotype is homozygous mutant + +*Complex genotype pattern* + +genotype pattern: 0/1, 0/0 ==> keep all variants for which the sample's genotype is either heterozygous or homozygous wildtype + +*Multiple sample-specific filters* + +Filter 1: genotype pattern: 0/0, Filter 2: genotype pattern 1/1: +==> keep all variants for which the first sample's gentoype is homozygous wildtype **and** the second sample's genotype is homozygous mutant + +*Combining sample-specific filter criteria* + +genotype pattern: 1/1, depth of coverage: 3, genotype quality: 9 +==> keep variants for which the sample's genotype is homozygous mutant **and** for which this genotype assignment is corroborated by a genotype quality score of at least 9 +**and** at least three reads from the sample cover the variant site + +**TIP:** + +As in the example above, genotype quality is typically most useful in combination with a genotype pattern. +It acts then, effectively, to make the genotype filter more stringent. + +@HELP_FOOTER@ + ]]> + +