# HG changeset patch
# User wolma
# Date 1513677244 18000
# Node ID f0f2795de2c7a54a16c72ca66ccb1bd7f76002f9

planemo upload for repository https://github.com/wm75/mimodd_galaxy_wrappers commit 528bcf3b769c7c73f119b2a176d19071f9ef5312

diff -r 000000000000 -r f0f2795de2c7 COPYING
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/COPYING	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,15 @@
+    MiModD (Mutation Identification in Model Organism Genomes using Desktop PCs)
+    Copyright (C) 2013, 2014, 2015, 2016, 2017  Wolfgang Maier
+
+    This program is free software: you can redistribute it and/or modify
+    it under the terms of the GNU General Public License as published by
+    the Free Software Foundation, either version 3 of the License, or
+    (at your option) any later version.
+
+    This program is distributed in the hope that it will be useful,
+    but WITHOUT ANY WARRANTY; without even the implied warranty of
+    MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+    GNU General Public License for more details.
+
+    You should have received a copy of the GNU General Public License
+    along with this program.  If not, see <http://www.gnu.org/licenses/>.
diff -r 000000000000 -r f0f2795de2c7 LICENSE
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/LICENSE	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,674 @@
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+option of following the terms and conditions either of that numbered
+version or of any later version published by the Free Software
+Foundation.  If the Program does not specify a version number of the
+GNU General Public License, you may choose any version ever published
+by the Free Software Foundation.
+
+  If the Program specifies that a proxy can decide which future
+versions of the GNU General Public License can be used, that proxy's
+public statement of acceptance of a version permanently authorizes you
+to choose that version for the Program.
+
+  Later license versions may give you additional or different
+permissions.  However, no additional obligations are imposed on any
+author or copyright holder as a result of your choosing to follow a
+later version.
+
+  15. Disclaimer of Warranty.
+
+  THERE IS NO WARRANTY FOR THE PROGRAM, TO THE EXTENT PERMITTED BY
+APPLICABLE LAW.  EXCEPT WHEN OTHERWISE STATED IN WRITING THE COPYRIGHT
+HOLDERS AND/OR OTHER PARTIES PROVIDE THE PROGRAM "AS IS" WITHOUT WARRANTY
+OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING, BUT NOT LIMITED TO,
+THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR
+PURPOSE.  THE ENTIRE RISK AS TO THE QUALITY AND PERFORMANCE OF THE PROGRAM
+IS WITH YOU.  SHOULD THE PROGRAM PROVE DEFECTIVE, YOU ASSUME THE COST OF
+ALL NECESSARY SERVICING, REPAIR OR CORRECTION.
+
+  16. Limitation of Liability.
+
+  IN NO EVENT UNLESS REQUIRED BY APPLICABLE LAW OR AGREED TO IN WRITING
+WILL ANY COPYRIGHT HOLDER, OR ANY OTHER PARTY WHO MODIFIES AND/OR CONVEYS
+THE PROGRAM AS PERMITTED ABOVE, BE LIABLE TO YOU FOR DAMAGES, INCLUDING ANY
+GENERAL, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES ARISING OUT OF THE
+USE OR INABILITY TO USE THE PROGRAM (INCLUDING BUT NOT LIMITED TO LOSS OF
+DATA OR DATA BEING RENDERED INACCURATE OR LOSSES SUSTAINED BY YOU OR THIRD
+PARTIES OR A FAILURE OF THE PROGRAM TO OPERATE WITH ANY OTHER PROGRAMS),
+EVEN IF SUCH HOLDER OR OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF
+SUCH DAMAGES.
+
+  17. Interpretation of Sections 15 and 16.
+
+  If the disclaimer of warranty and limitation of liability provided
+above cannot be given local legal effect according to their terms,
+reviewing courts shall apply local law that most closely approximates
+an absolute waiver of all civil liability in connection with the
+Program, unless a warranty or assumption of liability accompanies a
+copy of the Program in return for a fee.
+
+                     END OF TERMS AND CONDITIONS
+
+            How to Apply These Terms to Your New Programs
+
+  If you develop a new program, and you want it to be of the greatest
+possible use to the public, the best way to achieve this is to make it
+free software which everyone can redistribute and change under these terms.
+
+  To do so, attach the following notices to the program.  It is safest
+to attach them to the start of each source file to most effectively
+state the exclusion of warranty; and each file should have at least
+the "copyright" line and a pointer to where the full notice is found.
+
+    {one line to give the program's name and a brief idea of what it does.}
+    Copyright (C) {year}  {name of author}
+
+    This program is free software: you can redistribute it and/or modify
+    it under the terms of the GNU General Public License as published by
+    the Free Software Foundation, either version 3 of the License, or
+    (at your option) any later version.
+
+    This program is distributed in the hope that it will be useful,
+    but WITHOUT ANY WARRANTY; without even the implied warranty of
+    MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+    GNU General Public License for more details.
+
+    You should have received a copy of the GNU General Public License
+    along with this program.  If not, see <http://www.gnu.org/licenses/>.
+
+Also add information on how to contact you by electronic and paper mail.
+
+  If the program does terminal interaction, make it output a short
+notice like this when it starts in an interactive mode:
+
+    {project}  Copyright (C) {year}  {fullname}
+    This program comes with ABSOLUTELY NO WARRANTY; for details type `show w'.
+    This is free software, and you are welcome to redistribute it
+    under certain conditions; type `show c' for details.
+
+The hypothetical commands `show w' and `show c' should show the appropriate
+parts of the General Public License.  Of course, your program's commands
+might be different; for a GUI interface, you would use an "about box".
+
+  You should also get your employer (if you work as a programmer) or school,
+if any, to sign a "copyright disclaimer" for the program, if necessary.
+For more information on this, and how to apply and follow the GNU GPL, see
+<http://www.gnu.org/licenses/>.
+
+  The GNU General Public License does not permit incorporating your program
+into proprietary programs.  If your program is a subroutine library, you
+may consider it more useful to permit linking proprietary applications with
+the library.  If this is what you want to do, use the GNU Lesser General
+Public License instead of this License.  But first, please read
+<http://www.gnu.org/philosophy/why-not-lgpl.html>.
diff -r 000000000000 -r f0f2795de2c7 bamsort.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/bamsort.xml	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,97 @@
+<tool id="mimodd_sort" name="MiModD Sort" version="@MIMODD_WRAPPER_VERSION@">
+    <description>takes a SAM/BAM dataset and generates a coordinate/name-sorted copy</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command>
+	mimodd sort '$input.ifile' 
+	    -o '$ofile' --iformat $input.iformat --oformat $oformat $by_name
+    </command>
+  
+    <inputs>
+        <conditional name="input">
+            <param name="iformat" type="select" label="Input data format">
+                <option value="bam">bam</option>
+                <option value="sam">sam</option>
+            </param>
+            <when value="bam">
+                <param name="ifile" type="data" format="bam" 
+                label="BAM input dataset to sort" />
+            </when>
+            <when value="sam">
+                <param name="ifile" type="data" format="sam" 
+                label="SAM input dataset to sort" />
+            </when>
+        </conditional>
+        <param name="oformat" type="select" label="Output format for the sorted data">
+            <option value="bam">bam</option>
+            <option value="sam">sam</option>
+        </param>
+        <param name="by_name" type="boolean" truevalue="-n" falsevalue="" checked="false"
+        label="Sort by read names instead of coordinates"
+        help="A less common option, but necessary, e.g., if you want to re-align coordinate sorted paired-end reads with the MiModD Read Alignment Tool or other NGS mapping tools." />
+    </inputs>
+  
+    <outputs>
+        <data name="ofile" format="bam" 
+        label="Sorted output from MiModd ${tool.name} on ${on_string}">
+            <change_format>
+	            <when input="oformat" value="sam" format="sam" />
+            </change_format>
+        </data>
+    </outputs>
+    
+    <tests>
+        <test>
+            <conditional name="input">
+                <param name="iformat" value="sam" />
+                <param name="ifile" value="header_only.sam" />
+            </conditional>
+            <param name="oformat" value="bam" />
+            <assert_command>
+                <not_has_text text="-n" />
+            </assert_command>
+            <output name="ofile" ftype="bam" file="so_coordinate.bam" />
+        </test>
+        <test>
+            <conditional name="input">
+                <param name="iformat" value="bam" />
+                <param name="ifile" value="header_only.bam" />
+            </conditional>
+            <param name="oformat" value="sam" />
+            <param name="by_name" value="true" />
+            <assert_command>
+                <has_text text=" -n" />
+            </assert_command>
+            <output name="ofile" ftype="sam" file="so_queryname.sam" />
+        </test>
+    </tests>
+
+    <help><![CDATA[
+.. class:: infomark
+
+   **What it does**
+
+The tool sorts an aligned reads input dataset, typically by the reference
+genome coordinates that the reads have been mapped to.
+
+Coordinate-sorted input files are expected by most downstream MiModD tools, but
+note that the *MiModD Read Alignment* produces coordinate-sorted output by
+default and it is only necessary to sort files that come from other sources or
+from *MiModD Read Alignment* jobs with a custom sort order.
+
+The option *Sort by read names instead of coordinates* is useful if you want to
+re-align coordinate-sorted paired-end data. In *paired-end mode*, the
+*MiModD Read Alignment* tool expects the reads in the input file to be arranged
+in read pairs, *i.e.*, the forward read information of a pair must be followed
+immediately by its reverse mate information, which is typically not the case in
+coordinate-sorted data. Resorting such data by read names fixes this problem.
+
+@HELP_FOOTER@
+    ]]></help>
+    <expand macro="citations" />
+</tool>
+
diff -r 000000000000 -r f0f2795de2c7 cloudmap.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/cloudmap.xml	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,591 @@
+<tool id="mimodd_map" name="MiModD NacreousMap" version="@MIMODD_WRAPPER_VERSION@">
+    <description>maps phenotypically selected variants by multi-variant linkage analysis</description>
+    <macros>
+        <import>macros.xml</import>
+        <macro name="svd_unconditional">
+            <expand macro="hidden_algo_params" />
+            <expand macro="seqdict_param" />
+            <expand macro="bins" />
+            <conditional name="plotopts">
+                <param name="plots" type="select" label="graphical output settings">
+                    <option value="-p">Give me graphics.</option>
+                    <option value="">Do not generate graphs.</option>
+                </param>
+                <when value="">
+                </when>
+                <when value="-p">
+                    <expand macro="scatter_default" />
+                    <param name="show_kde" type="boolean" truevalue="" falsevalue="--no-kde" checked="true"
+                    label="show kde line in histogram plots"
+                    help="The tool can calculate a kernel density estimate for the linkage data based on a bin size of 10 kilobases and display it as a solid line in the histogram plots." />
+                    <param name="hylim" type="float" value="0.0"
+                    label="upper limit for the histogram y-axis (leave at 0.0 for automatic scaling)" />
+                    <param name="xlim" type="select" label="x-axis scaling">
+                        <option value="">preserve relative contig sizes</option>
+                        <option value="--fit-width">scale each contig to fit the plot width</option>
+                    </param>
+                    <expand macro="hist_colors" />
+                </when>
+            </conditional>
+        </macro>
+        <macro name="vaf_unconditional">
+            <expand macro="bins" />
+            <conditional name="plotopts">
+                <param name="plots" type="select" label="graphical output settings">
+                    <option value="-p">Give me everything (scatter plots and histograms)</option>
+                    <option value="--no-scatter -p">Generate only histograms</option>
+                    <option value="--no-hist -p">Generate only scatter plots</option>
+                    <option value="">Do not generate graphs.</option>
+                </param>
+                <when value="">
+                </when>
+                <when value="--no-scatter -p">
+                    <expand macro="scatter_default" />
+                    <param name="show_kde" type="boolean" truevalue="" falsevalue="--no-kde" checked="true"
+                    label="show kde line in histogram plots"
+                    help="The tool can calculate a kernel density estimate for the linkage data based on a bin size of 10 kilobases and display it as a solid line in the histogram plots." />
+                    <param name="hylim" type="float" value="0.0" 
+                    label="upper limit for the histogram y-axis (leave at 0.0 for automatic scaling)" />
+                    <param name="xlim" type="select" label="x-axis scaling">
+                        <option value="">preserve relative contig sizes</option>
+                        <option value="--fit-width">scale each contig to fit the plot width</option>
+                    </param>
+                    <expand macro="hist_colors" />
+                </when>
+                <when value="--no-hist -p">
+                    <expand macro="hist_default" />
+                    <param name="sylim" type="float" value="1.0" 
+                    label="upper limit for the scatter plot y-axis (default: 1)" />
+                    <param name="xlim" type="select" label="x-axis scaling">
+                        <option value="">preserve relative contig sizes</option>
+                        <option value="--fit-width">scale each contig to fit the plot width</option>
+                    </param>
+                    <param name="span" type="float" value="0.1" 
+                    label="span value to be used in calculating the Loess regression line through the scatter data (default=0.1, specify 0 to prevent calculation)" 
+                    help="smaller values give a more responsive curve that often picks up local evidence for tight linkage better, but too small values lead to plotting failures (in that case just rerun the tool with a larger value)." />
+                    <expand macro="scatter_colors" />
+                </when>
+                <when value="-p">
+                    <expand macro="plot_all" />
+                </when>
+            </conditional>
+        </macro>
+        <macro name="vac_unconditional">
+            <expand macro="bins" />
+            <conditional name="plotopts">
+                <param name="plots" type="select" label="graphical output settings">
+                    <option value="--no-scatter -p">Give me graphical output</option>
+                    <option value="">Do not generate graphs.</option>
+                </param>
+                <when value="">
+                </when>
+                <when value="--no-scatter -p">
+                    <expand macro="scatter_default" />
+                    <param name="show_kde" type="boolean" truevalue="" falsevalue="--no-kde" checked="true"
+                    label="show kde line in histogram plots"
+                    help="The tool can calculate a kernel density estimate for the linkage data based on a bin size of 10 kilobases and display it as a solid line in the histogram plots." />
+                    <param name="hylim" type="float" value="0.0" 
+                    label="upper limit for the histogram y-axis (leave at 0.0 for automatic scaling)" />
+                    <param name="xlim" type="select" label="x-axis scaling">
+                        <option value="">preserve relative contig sizes</option>
+                        <option value="--fit-width">scale each contig to fit the plot width</option>
+                    </param>
+                    <expand macro="hist_colors" />
+                </when>
+            </conditional>
+        </macro>
+        <macro name="hidden_algo_params">
+            <param name="sample" type="hidden" value="" />
+            <expand macro="hidden_vaf_algo_params" />
+            <param name="contrast_sample" type="hidden" value="" />
+        </macro>
+        <macro name="hidden_vaf_algo_params">
+            <param name="related_parent_sample" type="hidden" value="" />
+            <param name="unrelated_parent_sample" type="hidden" value="" />
+            <param name="infer_missing" type="hidden" value="" />
+        </macro>
+        <macro name="bins">
+            <repeat name="bin_sizes" default="0" min="0" title="bin sizes to analyze variants in (defaults to: 1Mb and 500Kb)" 
+            help="Values can be entered in bases (e.g., 1000000), kilobases (e.g., 500Kb) or megabases (e.g., 1Mb), but must be integral, i.e. no decimal numbers are allowed.">
+                <param name="bin_size" type="text">
+                     <validator type="empty_field" />
+                     <validator type="expression" message="Malformed bin size">not value or all(c.isdigit() or c in 'KMb' for c in value)</validator>
+                </param>
+            </repeat>
+        </macro>
+        <macro name="scatter_default">
+            <param name="sylim" type="hidden" value="" />
+            <param name="span" type="hidden" value="" />
+            <param name="scols" type="hidden" value="" />
+        </macro>
+        <macro name="hist_default">
+            <param name="show_kde" type="hidden" value="" />
+            <param name="hylim" type="hidden" value="" />
+            <param name="hcols" type="hidden" value="" />
+        </macro>
+        <macro name="hist_colors">
+            <repeat name="hcols" default="0" min="0" title="histogram colors"
+            help="For each bin size chosen above a histogram will be generated with its color selected from the list provided here (defaults to alternating darkgrey, red).">
+                <param name="hcolor" type="color" value="darkgrey">
+                    <sanitizer><valid><add value="#" /></valid></sanitizer>
+                </param>
+            </repeat>
+        </macro>
+        <macro name="scatter_colors">
+            <repeat name="scols" default="0" min="0" max="1" title="custom scatter plot colors">
+                <param name="pcol" type="color" value="black"
+                label="data points base color">
+                    <sanitizer><valid><add value="#" /></valid></sanitizer>
+                </param>
+                <param name="lcol" type="color" value="red"
+                label="regression line color">
+                    <sanitizer><valid><add value="#" /></valid></sanitizer>
+                </param>
+            </repeat>
+        </macro>
+        <macro name="plot_all">
+            <param name="show_kde" type="boolean" truevalue="" falsevalue="--no-kde" checked="true"
+            label="show kde line in histogram plots"
+            help="The tool can calculate a kernel density estimate for the linkage data based on a bin size of 10 kilobases and display it as a solid line in the histogram plots." />
+            <param name="hylim" type="float" value="0.0"
+            label="upper limit for the histogram y-axis (leave at 0.0 for automatic scaling)" />
+            <param name="sylim" type="float" value="1.0"
+            label="upper limit for the scatter plot y-axis (default: 1)" />
+            <param name="xlim" type="select" label="x-axis scaling">
+                <option value="">preserve relative contig sizes</option>
+                <option value="--fit-width">scale each contig to fit the plot width</option>
+            </param>
+            <param name="span" type="float" value="0.1"
+            label="span value to be used in calculating the Loess regression line through the scatter data (default=0.1, specify 0 to prevent calculation)" 
+            help="smaller values give a more responsive curve that often picks up local evidence for tight linkage better, but too small values lead to plotting failures (in that case just rerun the tool with a larger value)." />
+            <expand macro="hist_colors" />
+            <expand macro="scatter_colors" />
+        </macro>
+        <macro name="seqdict_param">
+            <conditional name="seqinfo_external">
+                <param name="source" type="select"
+                label="does this input provide contig name and size information?"
+                help="ALWAYS select 'yes' here if the input dataset was generated with MiModD. Input generated with third-party tools may or may not provide the information. If in doubt, execute the job with 'yes' selected and see if you are getting a corresponding error message.">
+                    <option value="">Yes</option>
+                    <option value="fasta">No, get the information from a reference genome in my history</option>
+                    <option value="cached">No, get the information from a built-in genome</option>
+                    <option value="seqdict">No, get the information from a CloudMap-style sequence dictionary in my history</option>
+                </param>
+                <when value="fasta">
+                    <param name="seqinfo" type="data" format="fasta"
+                    label="Reference genome to extract contig info from" />
+                </when>
+                <when value="cached">
+                    <param name="seqinfo" type="select"
+                    label="Reference genome to extract contig info from">
+                        <options from_data_table="all_fasta" />
+                    </param>
+                </when>
+                <when value="seqdict">
+                    <param name="seqinfo" type="data" format="tabular"
+                    label="CloudMap-style sequence dictionary to extract contig info from" />
+                </when>
+                <when value="">
+                </when>
+            </conditional>
+        </macro>
+        <macro name="test_tabular_out">
+            <output name="ofile" ftype="tabular">
+                <assert_contents>
+                    <has_text text="chrI" />
+                    <has_text text="chrII" />
+                    <has_text text="chrIII" />
+                    <has_text text="chrIV" />
+                    <has_text text="chrV" />
+                    <has_text text="chrX" />
+                    <has_text text="MtDNA" />
+                </assert_contents>
+            </output>
+        </macro>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command><![CDATA[
+    mimodd map ${opt.mode} '${opt.source.ifile}'
+      #if $str($opt.source.sample):
+        -m '${opt.source.sample}'
+      #end if
+      #if $str($opt.source.related_parent_sample):
+        -r '${opt.source.related_parent_sample}'
+      #end if
+      #if $str($opt.source.unrelated_parent_sample):
+        -u '${opt.source.unrelated_parent_sample}'
+      #end if
+      #if $str($opt.source.contrast_sample):
+        -c '${opt.source.contrast_sample}'
+      #end if
+        $opt.source.infer_missing
+        -o '$ofile'
+      #if $str($opt.source.seqinfo_external.source) in ("fasta", "seqdict"):
+        -s '${opt.source.seqinfo_external.seqinfo}'
+      #else if $str($opt.source.seqinfo_external.source) == "cached":
+        -s '${opt.source.seqinfo_external.seqinfo.fields.path}'
+      #end if
+      #if $len($opt.source.bin_sizes):
+        --bin-sizes
+        #for $size in $opt.source.bin_sizes:
+          '${size.bin_size}'
+        #end for
+      #end if
+      #if $str($opt.source.tabfile):
+        $str($opt.source.tabfile) '$tfile'
+      #end if
+      #if $str($opt.source.plotopts.plots):
+        $str($opt.source.plotopts.plots) '$pfile'
+        $opt.source.plotopts.show_kde
+        $str($opt.source.plotopts.xlim)
+        #if $str($opt.source.plotopts.hylim) and $opt.source.plotopts.hylim != 0.0:
+          --ylim-hist $str($opt.source.plotopts.hylim)
+        #end if
+        #if $str($opt.source.plotopts.hcols) and $len($opt.source.plotopts.hcols):
+          --hist-colors
+          #for $color in $opt.source.plotopts.hcols:
+            '${color.hcolor}'
+          #end for
+        #end if
+        #if $str($opt.source.plotopts.sylim):
+          --ylim-scatter $str($opt.source.plotopts.sylim)
+        #end if
+        #if $str($opt.source.plotopts.scols) and $len($opt.source.plotopts.scols):
+          #for $color_scheme in $opt.source.plotopts.scols:
+            --points-colors '${color_scheme.pcol}'
+            --loess-colors '${color_scheme.lcol}'
+          #end for
+        #end if
+        #if $str($opt.source.plotopts.span):
+          --loess-span $str($opt.source.plotopts.span)
+        #end if
+      #end if
+    ]]></command>
+  
+    <inputs>
+        <conditional name="opt">
+            <param name="mode" type="select" label="type of mapping analysis to perform" 
+            help="Select Simple Variant Density (SVD) Mapping to map mutations based on linked inheritance in near isogenic populations, Variant Allele Frequency (VAF) Mapping for bulk segregant analysis. Select Reprocess for rapidly replotting the result of a previous VAF analysis.">
+                <option value="SVD">Simple Variant Density Mapping</option>
+                <option value="VAF">Variant Allele Frequency Mapping</option>
+                <option value="VAC">Variant Allele Contrast Mapping</option>
+            </param>
+            <when value="SVD">
+                <conditional name="source">
+                    <param name="inputtype" type="select" label="data source to use">
+                        <option value="vcf">VCF file of variants (for de-novo mapping)</option>
+                        <option value="rep">per-variant report file (for remapping a previous analysis)</option>
+                    </param>
+                    <when value="vcf">
+                        <param name="ifile" type="data" format="vcf" 
+                        label="input file with variants to analyze" />
+                        <expand macro="svd_unconditional" />
+                        <param name="tabfile" type="select" 
+                        label="additional per-variant output file" 
+                        help="You can either choose to produce a tabular per-variant report, which is useful for fast replotting with different plot settings, or a VCF-like CloudMap-compatibility output that can be used as input for the older CloudMap EMS Variant Density Mapping tool as an alternative plotting tool.">
+                            <option value="">Do not generate per-variant output</option>
+                            <option value="-t">Tabular per-variant report</option>
+                            <option value="--cloudmap -t">CloudMap compatibility output</option>
+                        </param>
+                    </when>
+                    <when value="rep">
+                        <param name="ifile" type="data" format="tabular,csv" 
+                        label="input file with variants to analyze" />
+                        <param name="tabfile" type="hidden" value="" />
+                        <expand macro="svd_unconditional" />
+                    </when>
+                </conditional>
+            </when>
+            <when value="VAF">
+                <conditional name="source">
+                    <param name="inputtype" type="select" label="data source to use">
+                        <option value="vcf">VCF file of variants (for de-novo mapping)</option>
+                        <option value="rep">per-variant report file (for remapping a previous analysis)</option>
+                    </param>
+                    <when value="vcf">
+                        <param name="ifile" type="data" format="vcf" 
+                        label="input file with variants to analyze" />
+                        <expand macro="seqdict_param" />
+                        <param name="sample" type="text" 
+                        label="mapping sample name" help="the sample to perform mutation mapping for">
+                            <validator type="empty_field" />
+                            <expand macro="lex_sam_header" />
+                        </param>
+                        <param name="related_parent_sample" type="text" 
+                        label="name of the related parent sample" 
+                        help="the sample that provides variants present in your original mutant strain or in an ancestor (like the pre-mutagenesis strain); leave blank if not available">
+                            <expand macro="lex_sam_header" />
+                        </param>
+                        <param name="unrelated_parent_sample" type="text" 
+                        label="name of the unrelated parent sample" 
+                        help="the sample that provides variants present in the unrelated mapping strain (or in an ancestor of it) used in the mapping cross; leave blank if not available">
+                            <expand macro="lex_sam_header" />
+                        </param>
+                        <param name="infer_missing" type="boolean" truevalue="--infer-missing" falsevalue="" checked="false" 
+                        label="Infer alleles for missing parent" 
+                        help="if variant data for either the related or the unrelated parent strain is not available, the tool can try to infer the alleles present in that parent from the allele spectrum found in the mapping sample. Use with caution on carefully filtered variant lists only!" />
+                        <param name="contrast_sample" type="hidden" value="" />
+                        <expand macro="vaf_unconditional" />
+                        <param name="tabfile" type="select" 
+                        label="additional per-variant output file" 
+                        help="You can either choose to produce a tabular per-variant report, which is useful for fast replotting with different plot settings, or a VCF-like CloudMap-compatibility output that can be used as input for the older CloudMap Hawaiian Variant Mapping tool as an alternative plotting tool.">
+                            <option value="">Do not generate per-variant output</option>
+                            <option value="-t">Tabular per-variant report</option>
+                            <option value="--cloudmap -t">CloudMap compatibility output</option>
+                        </param>
+                    </when>
+                    <when value="rep">
+                        <param name="ifile" type="data" format="tabular,csv" 
+                        label="input file with variants to analyze" />
+                        <expand macro="seqdict_param" />
+                        <param name="tabfile" type="hidden" value="" />
+                        <expand macro="hidden_algo_params" />
+                        <expand macro="vaf_unconditional" />
+                    </when>
+                </conditional>
+            </when>
+            <when value="VAC">
+                <conditional name="source">
+                    <param name="inputtype" type="select"
+                    label="data source to use">
+                        <option value="vcf">VCF file of variants (for de-novo mapping)</option>
+                        <option value="rep">per-variant report file (for remapping a previous analysis)</option>
+                    </param>
+                    <when value="vcf">
+                        <param name="ifile" type="data" format="vcf"
+                        label="input file with variants to analyze" />
+                        <expand macro="seqdict_param" />
+                        <expand macro="hidden_vaf_algo_params" />
+                        <param name="sample" type="text"
+                        label="mapping sample name"
+                        help="the sample to perform mutation mapping for">
+                            <validator type="empty_field" />
+                            <expand macro="lex_sam_header" />
+                        </param>
+                        <param name="contrast_sample" type="text"
+                        label="name of the contrast sample"
+                        help="the sample that provides contrasting allele composition around the causal variant">
+                            <validator type="empty_field" />
+                            <expand macro="lex_sam_header" />
+                        </param>
+                        <expand macro="vac_unconditional" />
+                        <param name="tabfile" type="select"
+                        label="additional per-variant output file"
+                        help="You can choose to produce a tabular per-variant report, which is useful for fast replotting with different plot settings.">
+                            <option value="">Do not generate per-variant output</option>
+                            <option value="-t">Tabular per-variant report</option>
+                        </param>
+                    </when>
+                    <when value="rep">
+                        <param name="ifile" type="data" format="tabular,csv"
+                        label="input file with variants to analyze" />
+                        <expand macro="seqdict_param" />
+                        <param name="tabfile" type="hidden" value="" />
+                        <expand macro="hidden_algo_params" />
+                        <expand macro="vac_unconditional" />
+                    </when>
+                </conditional>
+            </when>
+        </conditional>
+    </inputs>
+    <outputs>
+        <data name="ofile" format="tabular" 
+        label="MiModD ${opt.mode} Mapping - binned variant counts for ${on_string}" />
+        <data name="tfile" format="tabular" 
+        label="MiModD ${opt.mode} Mapping - per-variant report for ${on_string}">
+            <filter>(opt['source']['tabfile'])</filter>
+        </data>
+        <data name="pfile" format="pdf" 
+        label="MiModD ${opt.mode} Mapping - linkage plots for ${on_string}">
+            <filter>(opt['source']['plotopts']['plots'])</filter>
+        </data>
+    </outputs>
+
+    <tests>
+        <test expect_num_outputs="1">
+            <conditional name="opt">
+                <param name="mode" value="SVD" />
+                <conditional name="source">
+                    <param name="inputtype" value="vcf" />
+                    <conditional name="plotopts">
+                        <param name="plots" value="" />
+                    </conditional>
+                    <param name="ifile" value="a.vcf" />
+                </conditional>
+            </conditional>
+            <expand macro="test_tabular_out" />
+        </test>
+        <test expect_num_outputs="2">
+            <conditional name="opt">
+                <param name="mode" value="VAF" />
+                <conditional name="source">
+                    <param name="inputtype" value="vcf" />
+                    <conditional name="plotopts">
+                        <param name="plots" value="-p" />
+                    </conditional>
+                    <param name="sample" value="ot266" />
+                    <param name="related_parent_sample" value="N2" />
+                    <param name="ifile" value="a.vcf" />
+                </conditional>
+            </conditional>
+            <expand macro="test_tabular_out" />
+            <output name="pfile" file="vaf_linkage.pdf" ftype="pdf"
+            compare="sim_size" delta="100000" />
+        </test>
+    </tests>
+    
+    <help><![CDATA[
+.. class:: infomark
+
+   **What it does**
+
+This is the most downstream tool in
+`mapping-by-sequencing analysis workflows in MiModD`_.
+
+It can be used to analyze and visualize the inheritance pattern of variants
+detected and selected with other MiModD tools or as an alternative (and more
+versatile) plotting engine for data generated with `CloudMap`_.
+
+-------------
+
+**Usage Modes:**
+
+This tool can be run in one of three different modes depending on the type of
+mapping analysis that should be performed:
+
+1) *Simple Variant Density (SVD) Mapping* mode analyzes the density of variants
+   along the reference genome by dividing each chromosome into regions of
+   user-defined size (bins) and counting the variants found in each bin. 
+    
+   All variants listed in the input file are analyzed in this mode, which means
+   that as input you will typically want to use filtered lists of variants (as
+   produced by the VCF Filter tool). 
+    
+   The aim of SVD analysis is to identify clusters of variants in an outcrossed
+   strain carrying a selectable unknown mutation, which is interpreted as
+   linkage between the corresponding genomic region and the unknown mutation.
+    
+   This mode corresponds roughly to EMS Variant Density Mapping in CloudMap.
+
+2) *Variant Allele Frequency (VAF) Mapping* mode analyzes the inheritance
+   pattern in cross-progeny at sites, at which the parents are homozygous for
+   different alleles. 
+    
+   The aim of VAF analysis is to identify clusters of variants with (near)
+   homozygous inheritance in a F2 (or later generation) population obtained
+   from a cross between a strain carrying a selectable unknown mutation and an
+   unrelated mapping strain. Such a cluster is interpreted as linkage between
+   the corresponding genomic region and the unknown mutation selected for in
+   the F2 generation. 
+    
+   This mode corresponds roughly to Hawaiian Variant Mapping in CloudMap, but
+   can simultaneously take into account non-reference alleles found in either
+   parent strain (CloudMap users may think of this as a combined Hawaiian
+   Variant and Variant Discovery Mapping analysis).
+
+3) *Variant Allele Contrast (VAC) Mapping* mode analyzes and visualizes the
+   divergence between two samples at all sites in the input dataset. It works
+   independent of any parent strain information and can be used if you have two
+   samples selected for contrary phenotypes, but also with a selected and a
+   non-selected sample.
+
+-------------
+
+**Input:**
+
+Valid input for this tool are VCF datasets (any such dataset in SVD mode, a
+MiModD-generated multi-sample VCF dataset in VAF and VAC modes) or a tabular
+report as generated by the CloudMap Hawaiian Variant Mapping tool.
+Alternatively, the tool can generate (in both modes) its own tabular reports,
+which can be used as input instead of the original VCF dataset, when rerunning
+the tool with different plotting parameters, to reduce analysis time. 
+
+-------------
+
+**Output:**
+
+The tool produces up to three output files:
+
+1) a default tabular report of binned variant counts that can be used to plot
+   the data with external software such as Excel,
+
+2) an optional pdf containing linkage plots, and
+  
+3) an optional tabular per-variant report, which can be configured to be either
+   valid input for the corresponding original CloudMap tool (for users who
+   really, really want to continue using CloudMap for plotting) or to be
+   reusable in fast reruns of the tool (which can be useful to experiment with
+   different plotting parameters).
+
+-------------
+
+**Settings:**
+
+1) Analysis settings
+
+   *bin size to analyze variants in* - determines the width of the regions
+   along each chromosome, in which variants are counted and analyzed together. 
+     
+   Several bin sizes can be specified and for each size you will get a
+   corresponding results section in the binned variant counts report and a
+   linkage histogram plot.
+      
+   *sample names (in VAF and VAC modes only)* - to analyze inheritance
+   patterns, the VAF and VAC modes need information about the relationship
+   between the samples defined in the input. While VAC mode simply requires
+   you to name the two contrasting samples for the analysis, the sample roles
+   in VAF mode are a bit more complicated to understand. Specifically:
+
+   The *mapping sample name* should be set to the name of the sample for which
+   the inheritance pattern is to be analyzed (the pooled progeny population). 
+
+   The *name of the related sample* should indicate the parent sample that
+   carried and brought in the unknown mutation to be mapped (or, alternatively,
+   a closely related ancestor). 
+
+   The *name of the unrelated sample* should be that of the other parent strain
+   used in the cross. 
+     
+   At least one of the parent samples MUST be specified, but if the input
+   contains variant information for both parents, they can be analyzed together
+   for higher mapping accuracy. If you are reanalyzing a tabular report from a
+   previous tool run or from CloudMap, the association between variants and
+   samples is already stored in the input dataset and cannot be specified
+   again.
+
+2) Graphical output settings
+
+   .. class:: warningmark
+  
+      To be able to generate plots, the system running MiModD needs to have the
+      statistical programming environment R and its Python interface rpy2
+      installed. Disable graphical output if this is not the case.
+
+   *y-axes scaling* - if you want to override the defaults
+
+   *x-axis scaling* - choose *preserve relative contig sizes* if you want the
+   largest chromosome to fit the page width and smaller chromosomes to appear
+   according to their relative size or choose *scale each contig to fit the
+   plot width* if all chromosomes should exploit the available space
+
+   *span value to be used in calculating the Loess regression line* - this
+   value determines the degree of smoothing of the regression line through the
+   scatterplot data. Information on loess regression and the loess span
+   parameter can be found at http://en.wikipedia.org/wiki/Local_regression.
+   
+   *colors used for plotting* - can be selected freely from the offered
+   palette. For histogram colors, the list of selected colors will be used to
+   provide the colors for the different histograms plotted. If less colors than
+   histograms (determined by the number of bin sizes selected) are specified,
+   colors from the list will be recycled.
+
+.. _CloudMap: https://usegalaxy.org/u/gm2123/p/cloudmap
+.. _mapping-by-sequencing analysis workflows in MiModD: http://mimodd.readthedocs.io/en/latest/nacreousmap.html
+.. _CloudMap-style sequence dictionary: http://mimodd.readthedocs.io/en/latest/fileformats.html#cloudmap-style-sequence-dictionary
+
+@HELP_FOOTER@
+    ]]></help>
+    <expand macro="citations" />
+</tool>
diff -r 000000000000 -r f0f2795de2c7 convert.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/convert.xml	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,439 @@
+<tool id="mimodd_convert" name="MiModD Convert" version="@MIMODD_WRAPPER_VERSION@">
+    <description>converts sequence data into different formats</description>
+    <macros>
+        <import>macros.xml</import>
+        <macro name="to_format">
+            <param name="oformat" type="select" label="to">
+            	<option value="sam">sam</option>
+	            <option value="bam">bam</option>
+	            <yield />
+	        </param>
+        </macro>
+        <macro name="se_selector" token_format="fastq">
+            <conditional name="input">
+                <param name="repr" type="select"
+                label="Single-end input data provided as">
+                    <option value="individual">Individual datasets</option>
+                    <option value="collection">Collection of datasets</option>
+                </param>
+                <when value="individual">
+                    <repeat name="input_data" title="fastq input datasets"
+                    default="1" min="1">
+	                    <param name="file1" type="data" format="@FORMAT@"
+	                    label="single-end read data"/>
+                    </repeat>
+                </when>
+                <when value="collection">
+                    <param name="input_data" type="data_collection"
+                    collection_type="list" format="fastq, fastq.gz"
+                    label="collection of single-end read input datasets" /> 
+                </when>
+            </conditional>
+            <param name="header" type="data" format="sam" label="Use Header File"
+            help="A SAM file with header information, as generated, for example, by the NGS Run Annotation Tool, that will be used to attach metainformation to the results file."/>
+
+        </macro>
+        <macro name="pe_selector" token_format="fastq">
+            <conditional name="input">
+                <param name="repr" type="select"
+                label="Paired-end input data provided as">
+                    <option value="individual">Individual datasets</option>
+                    <option value="collection">Paired collection</option>
+                    <option value="list_of_pairs">List of pairs</option>
+                </param>
+                <when value="individual">
+                    <repeat name="input_data" title="fastq input datasets"
+                    default="1" min="1">
+	                    <param name="file1" type="data" format="@FORMAT@"
+	                    label="first set of reads of paired-end data"/>
+	                    <param name="file2" type="data" format="@FORMAT@"
+	                    label="second set of reads of paired-end data"/>
+                    </repeat>
+                </when>
+                <when value="collection">
+                    <param name="input_data" type="data_collection"
+                    collection_type="paired" format="fastq, fastq.gz"
+                    label="paired input dataset collection" /> 
+                </when>
+                <when value="list_of_pairs">
+                    <param name="input_data" type="data_collection"
+                    collection_type="list:paired" format="fastq, fastq.gz"
+                    label="nested collection of paired input datasets" />
+                </when>
+            </conditional>
+            <param name="header" type="data" format="sam" label="Use Header File"
+            help="A SAM file with header information, as generated, for example, by the NGS Run Annotation Tool, that will be used to attach metainformation to the results file."/>
+
+        </macro>
+        <macro name="sam_bam_selector" token_format="sam">
+            <param name="input_data" type="data" format="@FORMAT@"
+            label="input dataset"/>
+            <param name="header" type="hidden" value="None"/>
+        </macro>
+        <macro name="fastq_output_choices">
+            <param name="split_on_rgs" type="hidden" value=""/>
+            <param name="reads_to_report" type="select" display="radio"
+            label="Types of reads to generate output for"
+            help="By default, the tool will generate two dataset collections, one for single reads found in the input file, and one for paired reads. If you know, in advance, that the input contains only single or only paired reads, you can prevent the generation of an empty dataset collection by selecting the appropriate option here.">
+                <option value="default">Single and paired reads</option>
+                <option value="single">Single reads only</option>
+                <option value="paired">Paired reads only</option>
+            </param>
+            <param name="multisegment_report" type="boolean" checked="false"
+            label="Generate additional output for multi-segment reads"
+            help="Multi-segment reads, as opposed to single- and two-segment/paired reads, are not present in typical NGS data, but allowed in SAM/BAM files. If you expect multi-segment reads in the input file, enable this option." />
+        </macro>
+        <macro name="bam_output_choices">
+            <param name="split_on_rgs" type="boolean" truevalue="--split-on-rgs" falsevalue="" checked="false"
+            label="Split output based on read group IDs"
+            help="If the input file contains reads from different read groups, write them to separate output files; implied automatically for conversions to fastq and gzipped fastq format"/>
+        </macro>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command><![CDATA[
+    ## Currently Galaxy does not autoconvert collections of fastq.gz files.
+    ## This tool wrapper fixes that by allowing fastq and fastq.gz as input
+    ## collection formats.
+    ## gz_input is then used as flag to indicate a fastq.gz input file
+    #set gz_input = False
+    #if $str($mode.output.split_on_rgs) or $str($mode.output.oformat) == "fastq" or $str($mode.output.oformat) == "gz":
+      mkdir converted_data &&
+    #end if
+  
+	mimodd convert 
+      #if $str($mode.iformat) in ("sam", "bam"):
+          '${mode.output.input_data}'
+      #else if $str($mode.iformat) == "fastq_pe":
+        #if $str($mode.output.input.repr) == "collection":
+          '$mode.output.input.input_data.forward' '$mode.output.input.input_data.reverse'
+          ## A paired collection - if the forward dataset is gzipped we assume
+          ## the reverse dataset is too.
+          #if $mode.output.input.input_data.forward.is_of_type('fastq.gz'):
+            #set gz_input = True
+          #end if
+        #else
+          #for $i in $mode.output.input.input_data
+            #if $str($mode.output.input.repr) == "individual":
+              '${i.file1}' '${i.file2}'
+            #else
+              '$i.forward' '$i.reverse'
+              ## A list:paired collection - let the last forward dataset
+              ## indicate whether input is gzipped
+              #if $i.forward.is_of_type('fastq.gz'):
+                #set gz_input = True
+              #end if
+            #end if
+          #end for
+        #end if
+      #else
+        #for $i in $mode.output.input.input_data
+          #if $str($mode.output.input.repr) == "collection":
+            '$i'
+            ## A simple collection of files - the last one determines
+            ## whether we assume gzipped input
+            #if $i.is_of_type('fastq.gz'):
+              #set gz_input = True
+            #end if
+          #else
+            '${i.file1}'
+          #end if
+        #end for
+      #end if
+	  #if $str($mode.output.header) != "None":
+		--header '$(mode.output.header)'
+	  #end if
+      #if $str($outputname) == "None":
+	    --ofile converted_data/read_group
+	  #else
+	    --ofile '$outputname'
+	  #end if
+	  #if $gz_input:
+	    ## a gzipped input dataset was found so lets set --iformat accordingly
+	    #if $str($mode.iformat) == "fastq_pe":
+	      --iformat gz_pe
+	    #else
+	      --iformat gz
+	    #end if
+	  #else
+	    --iformat $(mode.iformat)
+	  #end if
+	  --oformat $(mode.output.oformat)
+	  ${mode.output.split_on_rgs}
+
+    #if $str($mode.output.oformat) == "fastq" or $str($mode.output.oformat) == "gz":
+      &&
+      cd converted_data &&
+      for f in *2segments_r1.fastq.gz; do [ -f "\$f" ] || continue; mv "\$f" "`basename \$f _r1.fastq.gz`_forward.fastq.gz"; done &&
+      for f in *2segments_r2.fastq.gz; do [ -f "\$f" ] || continue; mv "\$f" "`basename \$f _r2.fastq.gz`_reverse.fastq.gz"; done &&
+      for f in *2segments_r1.fastq; do [ -f "\$f" ] || continue; mv "\$f" "`basename \$f _r1.fastq`_forward.fastq"; done &&
+      for f in *2segments_r2.fastq; do [ -f "\$f" ] || continue; mv "\$f" "`basename \$f _r2.fastq`_reverse.fastq"; done
+    #end if
+
+    ]]></command>
+  
+    <inputs>
+        <conditional name="mode">
+	        <param name="iformat" type="select" label="Convert from"
+	        help="Your choice will update the interface to display further choices appropriate for your type of input data.">
+        	    <option value="fastq">fastq: single-end (one file)</option>
+	            <option value="fastq_pe">fastq: paired-end (two files)</option>
+	            <option value="sam">sam</option>
+	            <option value="bam">bam</option>
+            </param>	
+            <when value="fastq">
+	            <conditional name="output">
+	                <expand macro="to_format" />
+	                <when value="sam">
+                        <expand macro="se_selector" format="fastq" />
+                        <param name="split_on_rgs" type="hidden" value=""/>
+                    </when>
+	                <when value="bam">
+                        <expand macro="se_selector" format="fastq" />
+                        <param name="split_on_rgs" type="hidden" value=""/>
+                    </when>
+                </conditional>
+	        </when>
+	        <when value="fastq_pe">
+	            <conditional name="output">
+	                <expand macro="to_format" />
+	                <when value="sam">
+                        <expand macro="pe_selector" format="fastq" />
+                        <param name="split_on_rgs" type="hidden" value=""/>
+                    </when>
+	                <when value="bam">
+                        <expand macro="pe_selector" format="fastq" />
+                        <param name="split_on_rgs" type="hidden" value=""/>
+                    </when>
+                </conditional>
+	        </when>
+	        <when value="sam">
+	            <conditional name="output">
+	                <expand macro="to_format">
+	                    <option value="fastq">fastq</option>
+	                    <option value="gz">gzipped fastq</option>
+	                </expand>
+	                <when value="fastq">
+	                    <expand macro="sam_bam_selector" format="sam" />
+	                    <expand macro="fastq_output_choices" />
+	                </when>
+	                <when value="gz">
+	                    <expand macro="sam_bam_selector" format="sam" />
+	                    <expand macro="fastq_output_choices" />
+	                </when>
+	                <when value="bam">
+	                    <expand macro="sam_bam_selector" format="sam" />
+	                    <expand macro="bam_output_choices" />
+	                </when>
+	                <when value="sam">
+	                    <expand macro="sam_bam_selector" format="sam" />
+	                    <expand macro="bam_output_choices" />
+	                </when>
+	            </conditional>
+	        </when>
+	        <when value="bam">
+	            <conditional name="output">
+	                <expand macro="to_format">
+	                    <option value="fastq">fastq</option>
+	                    <option value="gz">gzipped fastq</option>
+	                </expand>
+	                <when value="fastq">
+	                    <expand macro="sam_bam_selector" format="bam" />
+	                    <expand macro="fastq_output_choices" />
+	                </when>
+	                <when value="gz">
+	                    <expand macro="sam_bam_selector" format="bam" />
+	                    <expand macro="fastq_output_choices" />
+	                </when>
+	                <when value="bam">
+	                    <expand macro="sam_bam_selector" format="bam" />
+	                    <expand macro="bam_output_choices" />
+	                </when>
+	                <when value="sam">
+	                    <expand macro="sam_bam_selector" format="bam" />
+	                    <expand macro="bam_output_choices" />
+	                </when>
+	            </conditional>
+	        </when>
+        </conditional>
+    </inputs>
+  
+    <outputs>
+        <data name="outputname" format="bam"
+        label="Reads converted to ${mode.output.oformat} by ${tool.name} on ${on_string}">
+	        <change_format>
+	            <when input="mode.output.oformat" value="sam" format="sam" />
+	        </change_format>
+	        <filter>
+	            (mode['output']['oformat'] in ("bam", "sam") and not mode['output']['split_on_rgs'])
+	        </filter>
+        </data>
+        <collection name="bam_split_on_read_groups" type="list"
+        label="Reads converted to ${mode.output.oformat} by ${tool.name} on ${on_string}">
+            <discover_datasets pattern="__designation_and_ext__"
+            directory="converted_data" />
+	        <filter>
+	            (mode['output']['oformat'] in ('bam', 'sam') and mode['output']['split_on_rgs'])
+	        </filter>
+        </collection>
+        <collection name="fastq_SE_output_split_on_read_groups" type="list"
+        label="Single reads converted to fastq by ${tool.name} on ${on_string}">
+            <discover_datasets
+            pattern="(?P&lt;designation&gt;.+)_1segments_r1\.(?P&lt;ext&gt;fastq(\.gz)*)"
+            directory="converted_data" />
+	        <filter>
+	            (mode['output']['oformat'] in ('fastq', 'gz') and mode['output']['reads_to_report'] in ('default', 'single'))
+	        </filter>
+        </collection>
+        <collection name="fastq_PE_reads_split_on_read_groups" type="list:paired"
+        label="Read pairs converted to fastq by ${tool.name} on ${on_string}">
+            <discover_datasets
+            pattern="(?P&lt;identifier_0&gt;read_group_.+)_2segments_(?P&lt;identifier_1&gt;(forward|reverse))\.(?P&lt;ext&gt;fastq(\.gz)*)"
+            directory="converted_data" />
+	        <filter>
+	            (mode['output']['oformat'] in ('fastq', 'gz') and mode['output']['reads_to_report'] in ('default', 'paired'))
+	        </filter>
+        </collection>
+        <collection name="fastq_multi-segment_reads_split_on_read_groups" type="list:list"
+        label="Multi-segment reads converted to fastq by ${tool.name} on ${on_string}">
+            <discover_datasets
+            pattern="(?P&lt;identifier_0&gt;read_group_.+)_(?P&lt;identifier_1&gt;[3-9]|[1-9][0-9]+)segments_r[0-9]+\.(?P&lt;ext&gt;fastq(\.gz)*)"
+            directory="converted_data" />
+	        <filter>
+	            (mode['output']['oformat'] in ('fastq', 'gz') and mode['output']['multisegment_report'])
+	        </filter>
+        </collection>
+    </outputs>
+
+    <tests>
+        <test expect_num_outputs="1">
+            <conditional name="mode">
+                <param name="iformat" value="fastq" />
+                <conditional name="output">
+                    <param name="oformat" value="bam" />
+                    <conditional name="input">
+                        <param name="repr" value="individual" />
+                        <repeat name="input_data">
+                            <param name="file1"
+                            value="split_pair_reads_1.fastqsanger" />
+                        </repeat>
+                    </conditional>
+                    <param name="header" value="header_only.sam" />
+                </conditional>
+            </conditional>
+            <output name="outputname" file="reads_1_w_header.bam"
+            ftype="bam" />
+        </test>
+        <test expect_num_outputs="1">
+            <conditional name="mode">
+                <param name="iformat" value="fastq_pe" />
+                <conditional name="output">
+                    <param name="oformat" value="bam" />
+                    <conditional name="input">
+                        <param name="repr" value="individual" />
+                        <repeat name="input_data">
+                            <param name="file1"
+                            value="split_pair_reads_1.fastqsanger" />
+                            <param name="file2"
+                            value="split_pair_reads_2.fastqsanger" />
+                        </repeat>
+                    </conditional>
+                    <param name="header" value="header_only.sam" />
+                </conditional>
+            </conditional>
+            <output name="outputname" file="reads_1and2_w_header.bam"
+            ftype="bam" />
+        </test>
+        <test expect_num_outputs="1">
+            <conditional name="mode">
+                <param name="iformat" value="fastq_pe" />
+                <conditional name="output">
+                    <param name="oformat" value="bam" />
+                    <conditional name="input">
+                        <param name="repr" value="collection" />
+                        <param name="input_data">
+                            <collection type="paired">
+                                <element name="forward"
+                                value="split_pair_reads_1.fastqsanger" />
+                                <element name="reverse"
+                                value="split_pair_reads_2.fastqsanger" />
+                            </collection>
+                        </param>
+                    </conditional>
+                    <param name="header" value="header_only.sam" />
+                </conditional>
+            </conditional>
+            <output name="outputname" file="reads_1and2_w_header.bam" ftype="bam" />
+        </test>
+        <test expect_num_outputs="1">
+            <conditional name="mode">
+                <param name="iformat" value="bam" />
+                <conditional name="output">
+                    <param name="oformat" value="sam" />
+                    <param name="input_data" value="a.bam" />
+                </conditional>
+            </conditional>
+            <output name="outputname" file="a.sam" ftype="sam" />
+        </test>
+        <test>
+            <conditional name="mode">
+                <param name="iformat" value="sam" />
+                <conditional name="output">
+                    <param name="oformat" value="bam" />
+                    <param name="input_data" value="a.sam" />
+                    <param name="split_on_rgs" value="true" />
+                </conditional>
+            </conditional>
+            <output_collection name="bam_split_on_read_groups" type="list" count="2">
+                <element name="read_group_000" file="a_part1.bam" ftype="bam" />
+            </output_collection>
+        </test>
+    </tests>
+    
+    <help><![CDATA[
+.. class:: infomark
+
+   **What it does**
+
+The tool converts between different file formats used for storing
+next-generation sequencing data.
+
+As input file types it can handle fastq, SAM or BAM format, which it can
+convert to SAM or BAM format.
+
+**Notes:**
+
+1) The tool can convert fastq files representing data from paired-end
+sequencing runs to appropriate SAM/BAM format provided that the mate
+information is split over two fastq files in corresponding order.
+
+   **TIP:** If your paired-end data is arranged differently, you may look into
+   the *fastq splitter* and *fastq de-interlacer* tools for Galaxy from the
+   `Fastq Manipulation category`_ of the Galaxy Tool Shed to see if they can
+   convert your files to the expected format.
+   
+2) Merging partial fastq (or gzipped fastq) files into a single SAM/BAM file is
+supported both for single-end and paired-end data. Simply add additional input
+datasets and select the appropriate files (pairs of files in case of paired-end
+data).
+
+   Concatenation of SAM/BAM file during conversion is currently not supported.
+
+3) For input in fastq format a SAM header file providing run metadata
+**has to be specified**. The information in this file will be used as the
+header data of the new SAM/BAM file. You can use the *NGS Run Annotation* tool
+to generate a new header file for your data.
+
+   For input in SAM/BAM format the tool will simply copy the existing header
+   data to the new file. To modify the header of an existing SAM/BAM file, use
+   the *Reheader BAM file* tool instead.
+
+.. _Fastq Manipulation category: https://toolshed.g2.bx.psu.edu/repository/browse_repositories_in_category?id=310ff67d4caf6531
+.. _recipe for using gzipped fastq files in Galaxy: http://mimodd.readthedocs.org/en/latest/recipes.html#use-gzipped-fastq-files-in-galaxy
+.. _MiModD user guide: http://mimodd.readthedocs.org/en/latest
+
+@HELP_FOOTER@
+    ]]></help>
+    <expand macro="citations" />
+</tool>
diff -r 000000000000 -r f0f2795de2c7 covstats.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/covstats.xml	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,57 @@
+<tool id="mimodd_covstats" name="MiModD Coverage Statistics" 
+version="@MIMODD_WRAPPER_VERSION@">
+    <description>
+    calculates coverage statistics for a BCF file as generated by the MiModd Variant Calling tool
+    </description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command><![CDATA[
+    mimodd covstats '$ifile' --ofile '$ofile'
+    ]]></command>
+
+    <inputs>
+        <param name="ifile" type="data" format="bcf" label="BCF input file" 
+        help="Use the Variant Calling tool to generate input for this tool."/>
+    </inputs>
+    <outputs>
+        <data name="ofile" format="tabular" 
+        label="Coverage Statistics for ${on_string}"/>
+    </outputs>
+
+    <tests>
+        <test>
+            <param name="ifile" value="a.bcf" />
+            <output name="ofile" ftype="tabular">
+                <assert_contents>
+                    <has_n_columns n="3" />
+                    <has_line_matching expression="^chrI&#009;.+" />
+                    <has_line_matching expression="^chrII&#009;.+" />
+                    <has_line_matching expression="^chrIII&#009;.+" />
+                    <has_line_matching expression="^chrIV&#009;.+" />
+                    <has_line_matching expression="^chrV&#009;.+" />
+                    <has_line_matching expression="^chrX&#009;.+" />
+                    <has_line_matching expression="^MtDNA&#009;.+" />
+                </assert_contents>
+            </output>
+        </test>
+    </tests>
+    
+    <help><![CDATA[
+.. class:: infomark
+
+   **What it does**
+
+The tool takes as input a BCF file produced by the *Variant Calling* tool, and calculates per-chromosome read coverage from it.
+
+.. class:: warningmark
+
+   The tool treats genome positions missing from the BCF input as zero coverage, so it is safe to use ONLY with BCF files produced by the *Variant Calling* tool or through other commands that keep the information for all sites.
+
+@HELP_FOOTER@
+    ]]></help>
+    <expand macro="citations" />
+</tool>
diff -r 000000000000 -r f0f2795de2c7 deletion_predictor.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/deletion_predictor.xml	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,117 @@
+<tool id="mimodd_delcall" name="MiModD Deletion Calling (for PE data)" 
+version="@MIMODD_WRAPPER_VERSION@">
+    <description>
+    predicts deletions in one or more aligned paired-end read samples based on coverage of the reference genome and on insert sizes
+    </description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command><![CDATA[
+    mimodd delcall
+      #for $bam_file in $list_input
+        '$bam_file'
+      #end for
+        '$covfile' -o '$ofile'
+        --index-files 
+      #for $bam_file in $list_input
+        '${bam_file.metadata.bam_index}'
+      #end for
+        --max-cov $max_cov --min-size $min_size 
+        $include_uncovered 
+        $group_by_id 
+        --verbose
+    ]]></command>
+
+    <inputs>
+        <param name="list_input" type="data" multiple="true" format="bam"
+        label="Aligned reads input dataset(s)" />
+        <param name="covfile" type="data" format="bcf" 
+        label="BCF variant call dataset to extract coverage from" 
+        help="Use the MiModD Variant Calling Tool to generate this file."/>
+        <param name="group_by_id" type="boolean" truevalue="-i" falsevalue="" checked="false" 
+        label="group reads based on read group id only" 
+        help="If selected, reads from different read groups will be treated strictly separate. If turned off, read groups with identical sample names are used together for identifying uncovered regions, but are still treated separately for the prediction of deletions." />
+        <param name="include_uncovered" type="boolean" truevalue="-u" falsevalue="" checked="false" 
+        label="include low-coverage regions" 
+        help="If selected, regions that fulfill the coverage criteria below, but are not statistically significant deletions, will be included in the output." />  
+        <param name="max_cov" type="integer" value="0" 
+        label="maximal coverage allowed inside a low-coverage region (default: 0)" 
+        help="The maximal coverage at a site allowed to consider it as part of a low-coverage region" />
+        <param name="min_size" type="integer" value="100" 
+        label="minimal deletion size (default: 100)" 
+        help="A low-coverage region must consist of at least this number of consecutive bases below the maximal coverage to consider it in further analyses."/>
+    </inputs>
+
+    <outputs>
+        <data name="ofile" format="gff" />
+    </outputs>
+
+    <tests>
+        <test>
+            <param name="list_input" value="a.bam" />
+            <param name="covfile" value="a.bcf" />
+            <param name="include_uncovered" value="true" />
+            <assert_command>
+                <has_text text=" -u " />
+                <not_has_text text=" -i " />
+            </assert_command>
+            <output name="ofile" ftype="gff">
+                <assert_contents>
+                    <has_n_columns n="9" />
+                    <has_line_matching
+                    expression="^chrI&#009;MiModD&#009;Uncovered_Region.+" />
+                    <has_line_matching
+                    expression="^chrII&#009;MiModD&#009;Uncovered_Region.+" />
+                    <has_line_matching
+                    expression="^chrIII&#009;MiModD&#009;Uncovered_Region.+" />
+                    <has_line_matching
+                    expression="^chrIV&#009;MiModD&#009;Uncovered_Region.+" />
+                    <has_line_matching
+                    expression="^chrV&#009;MiModD&#009;Uncovered_Region.+" />
+                    <has_line_matching
+                    expression="^chrX&#009;MiModD&#009;Uncovered_Region.+" />
+                    <has_line_matching
+                    expression="^MtDNA&#009;MiModD&#009;Uncovered_Region.+" />
+                </assert_contents>
+            </output>
+        </test>
+    </tests>
+    
+    <help><![CDATA[
+.. class:: infomark
+
+   **What it does**
+
+The tool predicts deletions from paired-end data in a two-step process:
+
+1) It finds regions of low-coverage, i.e., candidate regions for deletions, by scanning a BCF file produced by the *Variant Calling* tool.
+
+   The *maximal coverage allowed inside a low-coverage region* and the *minimal deletion size* parameters are used at this step to define what is considered a low-coverage region.
+
+   .. class:: warningmark
+
+      The tool treats genome positions missing from the BCF input as zero coverage, so it is safe to use ONLY with BCF files produced by the *Variant Calling* tool or through other commands that keep the information for all sites.
+
+2) It assesses every low-coverage region statistically for evidence of it being a real deletion. **This step requires paired-end data** since it relies on shifts in the distribution of read pair insert sizes around real deletions.
+
+By default, the tool only reports Deletions, i.e., the subset of low-coverage regions that pass the statistical test.
+If *include low-coverage regions* is selected, regions that failed the test will also be reported.
+
+With *group reads based on read group id only* selected, as it is by default, grouping of reads into samples is done strictly based on their read group IDs.
+With the option deselected, grouping is done based on sample names in the first step of the analysis, i.e. the reads of all samples with a shared sample name are used to identify low-coverage regions.
+In the second step, however, reads will be regrouped by their read group IDs again, i.e. the statistical assessment for real deletions is always done on a per read group basis.
+
+**TIP:**
+Deselecting *group reads based on read group id only* can be useful, for example, if you have both paired-end and single-end sequencing data for the same sample.
+
+In this case, the two sets of reads will usually share a common sample name, but differ in their read groups.
+With grouping based on sample names, the single-end data can be used together with the paired-end data to identify low-coverage regions, thus increasing overall coverage and reliability of this step.
+Still, the assessment of deletions will use only the paired-end data (auto-detecting that the single-end reads do not provide insert size information).
+
+@HELP_FOOTER@
+    ]]></help>
+    <expand macro="citations" />
+</tool>
diff -r 000000000000 -r f0f2795de2c7 fileinfo.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/fileinfo.xml	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,102 @@
+<tool id="mimodd_info" name="MiModD File Information" version="@MIMODD_WRAPPER_VERSION@">
+    <description>provides summary reports for supported sequence data formats.</description>
+    <macros>
+        <import>macros.xml</import>
+        <xml name="test_with_formats" token_ifile="a.sam" token_oformat="text"
+        token_expectformat="txt">
+            <test>
+                <conditional name="input_selection">
+                    <param name="source" value="history" />
+                    <param name="ifile" value="@IFILE@" />
+                </conditional>
+                <param name="oformat" value="@OFORMAT@" />
+                <output name="ofile" ftype="@EXPECTFORMAT@">
+                    <assert_contents>
+                        <has_text text="chrI" />
+                        <has_text text="chrII" />
+                        <has_text text="chrIII" />
+                        <has_text text="chrIV" />
+                        <has_text text="chrV" />
+                        <has_text text="chrX" />
+                    </assert_contents>
+                </output>
+            </test>
+        </xml>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command><![CDATA[
+    mimodd info -o '$ofile' --verbose --oformat $oformat
+    #if str($input_selection.source) == "history":
+      '$input_selection.ifile'
+    #else:
+      '$input_selection.ifile.fields.path'
+    #end if
+    ]]></command>
+
+    <inputs>
+        <conditional name="input_selection">
+            <param name="source" type="select" label="Input is a">
+                <option value="history">Dataset in history</option>
+                <option value="cached">Genome on server</option>
+            </param>
+            <when value="history">
+                <param name="ifile" type="data" format="bam,sam,vcf,bcf,fasta"
+                label="input dataset" />
+            </when>
+            <when value="cached">
+                <param name="ifile" type="select" label="installed genome">
+                    <options from_data_table="all_fasta" />
+                </param>
+            </when>
+        </conditional>
+        <param name="oformat" type="select" display="radio"
+        label="output format">
+            <option value="txt">text</option>
+            <option value="html">html</option>
+        </param>
+    </inputs>
+
+    <outputs>
+        <data name="ofile" format="txt" label="Sample Info on ${on_string}">
+            <change_format>
+	            <when input="oformat" value="html" format="html"/>
+            </change_format>
+            <actions>
+                <conditional name="input_selection.source">
+                    <when value="cached">
+                        <action type="metadata" name="dbkey">
+                            <option type="from_data_table" name="all_fasta" column="1" offset="0">
+                                <filter type="param_value" ref="input_selection.ifile" column="0" />
+                            </option>
+                        </action>
+                    </when>
+                </conditional>
+            </actions>
+        </data>
+    </outputs>
+
+    <tests>
+        <expand macro="test_with_formats" ifile="a.bam" />
+        <expand macro="test_with_formats" ifile="a.sam" />
+        <expand macro="test_with_formats" ifile="a.bcf" />
+        <expand macro="test_with_formats" ifile="a.vcf" />
+        <expand macro="test_with_formats" ifile="a.fa" />
+        <expand macro="test_with_formats" ifile="a.bam" oformat="html"
+        expectformat="html" />
+    </tests>
+
+    <help><![CDATA[
+.. class:: infomark
+
+   **What it does**
+
+The tool inspects the input datasets and generates a report summarizing its contents.
+
+It autodetects and works with most file formats produced by MiModD, i.e., **SAM / BAM, vcf / bcf and fasta**, and produces a standardized report for all of them.
+
+@HELP_FOOTER@
+    ]]></help>
+    <expand macro="citations" />
+</tool>
diff -r 000000000000 -r f0f2795de2c7 macros.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/macros.xml	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,74 @@
+<macros>
+    <token name="@MIMODD_VERSION_REQUIRED@">0.1.8</token>
+    <token name="@MIMODD_REAL_VERSION@">0.1.8</token>
+    <token name="@MIMODD_WRAPPER_VERSION@">0.1.8_1</token>
+    <token name="@HELP_FOOTER@"><![CDATA[
+----
+
+.. class:: infomark
+
+   For **additional help** see these resources: 
+
+- The complete `MiModD User Guide <http://mimodd.readthedocs.io/en/doc0.1.8/usage_toc.html>`__
+
+- The `MiModD help forum <https://groups.google.com/forum/#!forum/mimodd>`__ reachable also via `email <mailto:mimodd@googlegroups.com>`__
+
+   ]]></token>
+    <xml name="requirements">
+        <requirements>
+            <requirement type="package" version="@MIMODD_VERSION_REQUIRED@">MiModD</requirement>
+            <yield />
+        </requirements>
+    </xml>
+    <xml name="stdio">
+        <stdio>
+            <exit_code range="1:" />
+        </stdio>
+    </xml>
+    <xml name="version_command">
+        <version_command><![CDATA[mimodd version -q]]></version_command>
+    </xml>
+    <xml name="citations">
+        <citations>
+            <citation type="bibtex">
+@misc{
+maier_mimodd_2014,
+title = {{MiModD} - {Mutation} {Identification} in {Model} {Organism} {Genomes}},
+shorttitle = {{MiModD}},
+url = {https://sourceforge.net/projects/mimodd/},
+author = {Maier, Wolfgang and Moos, Katharina and Seifert, Mark and Baumeister, Ralf},
+year = {2014}, publisher={SourceForge.net} }
+            </citation>
+        </citations>
+    </xml>
+    <macro name="YYYY-MM-DD_format_validator">
+        <validator type="expression" message="YYYY-MM-DD date format required">not value or (len(value.split('-')) == 3 and all(part.isdigit() and len(part)==expect_len for part, expect_len in zip(value.split('-'), [4,2,2])))</validator>
+    </macro>
+    <macro name="known_sequencing_platform options">
+        <option value="ILLUMINA">ILLUMINA</option>
+        <option value="PACBIO">PACBIO</option>
+        <option value="IONTORRENT">IONTORRENT</option>
+        <option value="ONT">ONT</option>
+        <option value="LS454">LS454</option>
+        <option value="SOLID">SOLID</option>
+        <option value="HELICOS">HELICOS</option>
+        <option value="CAPILLARY">CAPILLARY</option>
+    </macro>
+    <macro name="lex_sam_header" token_message="SAM header data may not contain non-ASCII characters.">
+        <validator type="expression" message="@MESSAGE@">not value or all(ord(c) &lt; 127 for c in value)</validator>
+        <sanitizer invalid_char="">
+            <valid initial="string.printable">
+                <remove preset="string.whitespace" value="&#39;" />
+                <add value=" " />
+            </valid>
+            <mapping initial="none">
+                <add source="&#39;" target="&#39;&quot;&#39;&quot;&#39;" />
+                <add source="&#09;" target="&#32;" />
+                <add source="&#10;" target="&#32;" />
+                <add source="&#13;" target="&#32;" />
+            </mapping>
+        </sanitizer>
+    </macro>
+</macros>
+
+
diff -r 000000000000 -r f0f2795de2c7 rebase.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/rebase.xml	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,75 @@
+<tool id="mimodd_rebase" name="MiModD Rebase Sites" 
+version="@MIMODD_WRAPPER_VERSION@">
+    <description>from a VCF file</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command><![CDATA[
+	mimodd rebase '$ifile'
+	  '$chainfile'
+	  $reverse
+	  --ofile '$output_vcf'
+	  --filter $filter
+	  --verbose
+    ]]></command>
+
+    <inputs>
+        <param name="ifile" type="data" format="vcf" label="VCF input file"
+        help="the VCF file to rebase"/>
+        <param name="chainfile" type="data" format="tabular" label="Input chainfile"
+        help="the UCSC chain file to calculate new coordinates from"/>
+        <param name="reverse" type="boolean" truevalue="-r" falsevalue="" checked="false"
+        label="reverse the target and query sites of the initial chainfile"
+        help="swap the genome versions specified in the chain file, i.e., assume the coordinates in the input file are based on the chain file target genome version and should be mapped to the source genome version" />
+        <param name="filter" type="select" display="radio"
+        label="Ambiguously mapping variants"
+        help="How to treat variants that map to more than one position in the target coordinate system" >
+            <option value="unique">Ignore</option>
+            <option value="best">Report only the mapping with the highest score</option>
+            <option value="all">Report all possible mappings</option>
+        </param>
+    </inputs>
+    <outputs>
+        <data name="output_vcf" format="vcf"
+        label="Variants rebased with MiModd from ${on_string}"/>
+    </outputs>
+
+    <tests>
+        <test>
+            <param name="ifile" value="a.vcf" />
+            <param name="chainfile" value="ce11ToCe10.over.chain" />
+            <assert_command>
+                <not_has_text text=" -r" />
+            </assert_command>
+            <output name="output_vcf" file="rebased.vcf" ftype="vcf"
+            lines_diff="0" />
+        </test>
+        <test>
+            <param name="ifile" value="rebased.vcf" />
+            <param name="chainfile" value="ce11ToCe10.over.chain" />
+            <param name="reverse" value="true" />
+            <assert_command>
+                <has_text text=" -r" />
+            </assert_command>
+            <output name="output_vcf" file="a.vcf" ftype="vcf"
+            lines_diff="20" />
+        </test>
+    </tests>
+    
+    <help><![CDATA[
+.. class:: infomark
+
+   **What it does**
+   
+The tool takes as input a VCF file like the ones produced by the
+**Extract Variant** tool and a Genome Browser chain format, and maps the
+variant positions found in the VCF file to a different reference genome
+coordinate system according to the mapping defined in a UCSC chain file.
+
+@HELP_FOOTER@
+    ]]></help>
+    <expand macro="citations" />
+</tool>
diff -r 000000000000 -r f0f2795de2c7 reheader.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/reheader.xml	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,300 @@
+<tool id="mimodd_reheader" name="MiModD Reheader" version="@MIMODD_WRAPPER_VERSION@">
+    <description>
+    takes a BAM file and generates a copy with the original header (if any) replaced or modified by that found in a template SAM file
+    </description>
+    <macros>
+        <import>macros.xml</import>
+        <macro name="getreadgroupinfo">
+            <conditional name="rginfo">
+                <param name="source" type="select"
+                label="source of new read-group information">
+                    <option value="from_file">existing SAM file</option>
+                    <option value="from_form">input form</option>
+                </param>
+                <when value="from_file">
+                    <param name="data" type="data" format="sam"
+                    label="read-group template file in SAM format"
+                    help="use the read group information found in this file" />
+                    <repeat name="rg" title="custom read-group mapping" default="0" min="0"
+                    help="read-group information found in the input file, by default, gets updated / replaced with information from template file read-groups with matching IDs. Alternatively, you may specify explicit read-group mappings below.">
+                        <param name="source_id" type="text"
+                        label="modify input file information for read-group ID (will create the read-group if it does not exist)" />
+                        <param name="rg_id" type="text"
+                        label="with template file information for read-group ID" />
+                    </repeat>
+                </when>
+                <when value="from_form">
+                    <repeat name="rg" title="new read-group info" default="1" min="1">
+                        <param name="source_id" type="text"
+                        label="read-group ID (will create the read-group if it does not exist)"
+                        help="required field">
+                            <validator type="empty_field" />
+                            <expand macro="lex_sam_header" />
+                        </param>
+                        <param name="rg_id" type="hidden" value="" />
+                        <param name="rg_sm" type="text" label="sample name"
+                        help="required field">
+                            <validator type="empty_field" />
+                            <expand macro="lex_sam_header" />
+                        </param>
+                        <param name="rg_ds" type="text" label="description">
+                            <expand macro="lex_sam_header" />
+                        </param>
+                        <param name="rg_date" type="text"
+                        label="date (YY-MM-DD format) the run was produced">
+                            <expand macro="YYYY-MM-DD_format_validator" />
+                        </param>
+                        <param name="rg_cn" type="text"
+                        label="name of sequencing center">
+                            <expand macro="lex_sam_header" />
+                        </param>
+                        <param name="rg_lb" type="text" label="read-group library">
+                            <expand macro="lex_sam_header" />
+                        </param>
+                        <param name="rg_pl" type="text"
+                        label="platform/technology used to produce the reads">
+                            <expand macro="known_sequencing_platform options" />
+                            <expand macro="lex_sam_header" />
+                        </param>
+                        <param name="rg_pi" type="text"
+                        label="predicted median insert size">
+                            <validator type="expression" message="an integer number is required">not value or value.isdigit()</validator>
+                        </param>
+                        <param name="rg_pu" type="text" 
+                        label="platform unit; unique identifier">
+                            <expand macro="lex_sam_header" />
+                        </param>
+                    </repeat>    
+                </when>
+            </conditional>
+        </macro>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command><![CDATA[
+    #if ($str($rg.treat_rg) != "ignore" and $str($rg.rginfo.source) == "from_form") or $str($co.treat_co) != "ignore":
+      mimodd header
+      #if $str($rg.treat_rg) != "ignore" and $str($rg.rginfo.source) == "from_form":
+        #for $rginfo in $rg.rginfo.rg
+      	  #if $str($rginfo.source_id):
+            --rg-id '${rginfo.source_id}'
+          #end if
+          #if $str($rginfo.rg_sm):
+      	    --rg-sm '${rginfo.rg_sm}'
+          #end if
+	      #if $str($rginfo.rg_cn):
+		    --rg-cn '${rginfo.rg_cn}'
+		  #else:
+		    --rg-cn ""
+	      #end if
+	      #if $str($rginfo.rg_ds):
+		    --rg-ds '${rginfo.rg_ds}'
+		  #else:
+		    --rg-ds ""
+	      #end if	
+	      #if $str($rginfo.rg_date):
+		    --rg-dt '${rginfo.rg_date}'
+		  #else:
+		    --rg-dt ""
+	      #end if
+	      #if $str($rginfo.rg_lb):
+		    --rg-lb '${rginfo.rg_lb}'
+		  #else:
+		    --rg-lb ""
+	      #end if
+	      #if $str($rginfo.rg_pl):
+		    --rg-pl '${rginfo.rg_pl}'
+		  #else:
+		    --rg-pl ""
+	      #end if
+	      #if $str($rginfo.rg_pi):
+		    --rg-pi '${rginfo.rg_pi}'
+		  #else:
+		    --rg-pi ""
+	      #end if
+	      #if $str($rginfo.rg_pu):
+		    --rg-pu '${rginfo.rg_pu}'
+		  #else:
+		    --rg-pu ""
+	      #end if
+	    #end for
+	  #end if
+	  #if $str($co.treat_co) != "ignore":
+	    --co
+	    #for $comment in $co.coinfo
+          #if $str($comment.line):
+	        '${comment.line}'
+	      #end if
+	    #end for
+	  #end if
+      | 
+    #end if
+    mimodd reheader '$inputfile' --sq ignore --rg ${rg.treat_rg}
+    #if $str($rg.treat_rg) != "ignore":
+      #if $str($rg.rginfo.source) == "from_file":
+        '${rg.rginfo.data}'
+      #else:
+        - 
+      #end if
+      #for $rgmapping in $rg.rginfo.rg
+        #if $str($rgmapping.source_id) and $str($rgmapping.rg_id):
+          '$str($rgmapping.source_id)' : '$str($rgmapping.rg_id)'
+        #end if
+      #end for     
+    #end if
+    
+    --co ${co.treat_co}
+    #if $str($co.treat_co) != "ignore":
+      -
+    #end if
+
+    #set $restr = ""
+    #for $rename in $rg_renaming
+      #set $restr = $restr + ($str($rename.from) and $str($rename.to) and "'" + $str($rename.from) + "' : '" + $str($rename.to) + "' ")
+    #end for
+    #if $restr
+      --rgm $restr
+    #end if
+ 
+    #set $restr = ""
+    #for $rename in $sq_renaming
+      #set $restr = $restr + ($str($rename.from) and $str($rename.to) and "'" + $str($rename.from) + "' : '" + $str($rename.to) + "' ")
+    #end for
+    #if $restr
+      --sqm $restr
+    #end if
+
+    -o '$output'  
+    ]]></command>
+    
+    <inputs>
+        <param name="inputfile" type="data" format="bam"
+        label="input dataset in BAM format" help="the file to reheader." />
+        <conditional name="rg">
+            <param name="treat_rg" type="select"
+            label="modify read-group information?"
+            help="Replace mode will ignore ALL existing read group information in the input file and use ONLY template information, Update mode will COPY existing input file information and UPDATE it with template information; choose No, ... to leave read-group information alone.">
+                <option value="ignore">No, do not change read-groups.</option>
+                <option value="update">Yes, update existing information</option>
+                <option value="replace">Yes, replace existing information</option>
+            </param>
+            <when value="update">
+                <expand macro="getreadgroupinfo" />
+            </when>
+            <when value="replace">
+                <expand macro="getreadgroupinfo" />
+            </when>
+            <when value="ignore">
+            </when>
+        </conditional>
+        <conditional name="co">
+            <param name="treat_co" type="select"
+            label="modify comments in the input file?">
+                <option value="ignore">No, do not change comments.</option>
+                <option value="update">Yes, append new comments to existing ones</option>
+                <option value="replace">Yes, replace all existing comments</option>
+            </param>
+            <when value="update">
+                <repeat name="coinfo" title="comment line" default="0" min="0">
+                    <param name="line" type="text" size="80">
+                        <expand macro="lex_sam_header" />
+                    </param>
+                </repeat>
+            </when>
+            <when value="replace">
+                <repeat name="coinfo" title="comment line" default="0" min="0">
+                    <param name="line" type="text" size="80">
+                        <expand macro="lex_sam_header" />
+                    </param>
+                </repeat>
+            </when>
+            <when value="ignore">
+            </when>
+        </conditional>
+        <repeat name="rg_renaming" title="rename read-group" default="0" min="0"
+        help="Warning: changing read-group IDs may increase job runtime substantially.">
+            <param name="from" type="text" size="30" label="old name"
+            help="as it appears in the current input file header">
+                <expand macro="lex_sam_header" />
+            </param>
+            <param name="to" type="text" size="30" label="new name">
+                <expand macro="lex_sam_header" />
+            </param>
+        </repeat>
+        <repeat name="sq_renaming" title="rename sequence" default="0" min="0"
+        help="Warning: changing sequence names may increase job runtime substantially.">
+            <param name="from" type="text" size="30" label="old name"
+            help="as it appears in the current input file header">
+                <expand macro="lex_sam_header" />
+            </param>
+            <param name="to" type="text" size="30" label="new name">
+                <validator type="expression" message="Sequence names may not contain non-ASCII, whitespace, non-printable characters, nor any of: &lt;&gt;[]*;=,">all((32 &lt; ord(c) &lt; 127 and c not in '&lt;&gt;[]*;=,') for c in value)</validator>
+                <expand macro="lex_sam_header" />
+            </param>
+        </repeat>
+    </inputs>
+  
+    <outputs>
+        <data name="output" format="bam"
+        label="(Re)headered bam file from MiModd ${tool.name} on ${on_string}" />
+    </outputs>
+
+    <tests>
+        <test>
+            <param name="inputfile" value="a.bam" />
+            <conditional name="co">
+                <param name="treat_co" value="update" />
+                <repeat name="coinfo">
+                    <param name="line" value="ceterum censeo ..." />
+                </repeat>
+            </conditional>
+            <output name="output" file="a.bam" ftype="bam" lines_diff="1" />
+        </test>
+        <test>
+            <param name="inputfile" value="header_only.bam" />
+            <conditional name="rg">
+                <param name="treat_rg" value="update" />
+                <conditional name="rginfo">
+                    <param name="source" value="from_form" />
+                    <repeat name="rg">
+                        <param name="source_id" value="000" />
+                        <param name="rg_sm" value="Bristol" />
+                    </repeat>
+                </conditional>
+            </conditional>
+            <output name="output" file="header_only.bam" ftype="bam" lines_diff="2" />
+        </test>
+        <test>
+            <param name="inputfile" value="header_only.bam" />
+            <conditional name="rg">
+                <param name="treat_rg" value="update" />
+                <conditional name="rginfo">
+                    <param name="source" value="from_form" />
+                    <repeat name="rg">
+                        <param name="source_id" value="001" />
+                        <param name="rg_sm" value="Hawaiian" />
+                    </repeat>
+                </conditional>
+            </conditional>
+            <output name="output" file="header_only.bam" ftype="bam" lines_diff="1" />
+        </test>        
+    </tests>
+    
+    <help><![CDATA[
+.. class:: infomark
+
+   **What it does**
+
+The tool generates a copy of the BAM input file with a modified header (i.e., metadata). 
+
+It can update or replace read-group information (i.e., information about the samples in the file), add or replace comment lines, and rename reference sequences declared in the header.
+
+The tool ensures that the resulting BAM file is valid and can be further processed by other MiModD tools and standard software like samtools. It aborts with an error message if a valid BAM file cannot be generated with the user-specified settings.
+ 
+The template information used to modify or replace the input file metadata is provided through forms or, in the case of read-group information, can be taken from an existing SAM file as can be generated, for example, with the *NGS Run Annotation* tool.
+
+@HELP_FOOTER@
+    ]]></help>
+    <expand macro="citations" />
+</tool>
diff -r 000000000000 -r f0f2795de2c7 sam_header.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/sam_header.xml	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,123 @@
+<tool id="mimodd_header" name="MiModD Run Annotation" version="@MIMODD_WRAPPER_VERSION@">
+    <description>
+    writes run metadata in SAM format for attaching it to sequenced reads data
+    </description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command><![CDATA[
+  	mimodd header
+	    --rg-id '$rg_id'
+	    --rg-sm '$rg_sm'
+	  #if $str($rg_cn):
+		--rg-cn '$rg_cn'
+	  #end if
+	  #if $str($rg_ds):
+		--rg-ds '$rg_ds'
+	  #end if	
+	  #if $str($rg_date):
+		--rg-dt '$rg_date'
+	  #end if
+	  #if $str($rg_lb):
+		--rg-lb '$rg_lb'
+	  #end if
+	  #if $str($rg_pl):
+		--rg-pl '$rg_pl'
+	  #end if
+	  #if $str($rg_pi):
+		--rg-pi '$rg_pi'
+	  #end if
+	  #if $str($rg_pu):
+		--rg-pu '$rg_pu'
+	  #end if
+	    --ofile '$ofile'
+
+    ]]></command>
+
+    <inputs>
+        <param name="rg_id" type="text" size="80"
+        label="read-group ID (required)">
+            <validator type="empty_field" />
+            <expand macro="lex_sam_header" />
+        </param>
+        <param name="rg_sm" type="text" size="80"
+        label="sample name (required)">
+            <validator type="empty_field" />
+            <expand macro="lex_sam_header" />
+        </param>
+        <param name="rg_ds" type="text" size="80" label="description">
+            <expand macro="lex_sam_header" />
+        </param>
+        <param name="rg_date" type="text"
+        label="date (YYYY-MM-DD) the run was produced">
+            <expand macro="YYYY-MM-DD_format_validator" />
+        </param>
+        <param name="rg_cn" type="text" size="80"
+        label="name of sequencing center">
+            <expand macro="lex_sam_header" />
+        </param>
+        <param name="rg_lb" type="text" size="80" label="read-group library">
+            <expand macro="lex_sam_header" />
+        </param>
+        <param name="rg_pl" type="text"
+        label="platform/technology used to produce the reads">
+            <expand macro="known_sequencing_platform options" />
+            <expand macro="lex_sam_header" />
+        </param>
+        <param name="rg_pi" type="text" label="predicted median insert size">
+            <validator type="expression" message="an integer number is required">not value or value.isdigit()</validator>
+        </param>
+        <param name="rg_pu" type="text" size="80"
+        label="platform unit; unique identifier">
+            <expand macro="lex_sam_header" />
+        </param>
+    </inputs>
+
+    <outputs>
+        <data name="ofile" format="sam" 
+        label="${rg_sm} (${rg_id}) header information from MiModd ${tool.name} on ${on_string}"/>
+    </outputs>
+    
+    <tests>
+        <test>
+            <param name="rg_id" value="000" />
+            <param name="rg_sm" value="N2" />
+            <param name="rg_ds" value="C. elegans wt" />
+            <param name="rg_date" value="2017-06-27" />
+            <param name="rg_cn" value="ABC" />
+            <param name="rg_lb" value="XYZ" />
+            <param name="rg_pl" value="ILLUMINA" />
+            <param name="rg_pi" value="400" />
+            <param name="rg_pu" value="SEQ123" />
+            <output name="ofile" ftype="sam" file="header_only.sam" />
+        </test>
+    </tests>
+
+    <help><![CDATA[
+.. class:: infomark
+
+   **What it does**
+
+This tool takes user-provided information about a next-generation sequencing
+run and constructs a valid SAM format header from it.
+
+The resulting dataset can be used by the *MiModD Convert*, *MiModD Reheader*
+and the *MiModD Read Alignment* tool to add run metadata to sequenced reads
+input datasets (or to overwrite pre-existing information).
+
+**Note:**
+
+**MiModD requires run metadata for every input file at the Alignment step !**
+
+**Tip:**
+
+While you can do Alignments from fastq file format by providing a custom header file directly to the *SNAP Read Alignment* tool, we **recommend** you to first convert all input files to and archive all datasets in SAM/BAM format with appropriate header information prior to any downstream analysis. Although a bit more time-consuming, this practice protects against information loss and ensures that the input datasets will remain useful for others in the future.
+
+@HELP_FOOTER@
+    ]]></help>
+    <expand macro="citations" />
+</tool>
+	
diff -r 000000000000 -r f0f2795de2c7 snp_caller_caller.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/snp_caller_caller.xml	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,191 @@
+<tool id="mimodd_varcall" name="MiModD Variant Calling"
+version="@MIMODD_WRAPPER_VERSION@">
+    <description>
+    generates a BCF file of position-specific variant likelihoods and coverage information based on a reference sequence and reads aligned against it
+    </description>
+    <macros>
+        <import>macros.xml</import>
+        <macro name="test_mentions_samples">
+            <assert_stdout>
+                <has_text_matching expression="000.+N2" />
+                <has_text_matching expression="266-1.+ot266" />
+            </assert_stdout>
+        </macro>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command><![CDATA[
+	mimodd varcall
+	  #if str($reference.source) == "cached":
+        '$reference.genome.fields.path'
+      #else:
+        '$reference.genome'
+      #end if
+	  #for $input_file in $list_input
+        '$input_file'
+      #end for
+        --index-files 
+      #for $input_file in $list_input
+        '${input_file.metadata.bam_index}'
+      #end for
+	    --ofile '$ofile'
+	    $group_by_id
+	    $adv_settings.md5_check
+        --max-depth $adv_settings.max_depth
+        --verbose
+        --quiet
+    ]]></command>
+
+    <inputs>
+        <conditional name="reference">
+            <param name="source" type="select"
+            label="Will you select a reference genome from your history or use a built-in genome?">
+                <option value="cached">Use a built-in genome</option>
+                <option value="history">Use a genome from my history</option>
+            </param>
+            <when value="cached">
+                <param name="genome" type="select"
+                label="reference genome"
+                help="The fasta reference genome that variants should be called against.">
+                    <options from_data_table="all_fasta" />
+                </param>
+            </when>
+            <when value="history">
+                <param name="genome" type="data" format="fasta"
+                label="reference genome"
+                help="The fasta reference genome that variants should be called against."/>
+            </when>
+        </conditional>
+        <param name="list_input" type="data" multiple="true" format="bam"
+        label="Aligned reads input dataset(s)"
+        help="Select at least one dataset to call variants on. If you select several datasets or a dataset collection, this tool will perform joint variant calling on all of them and produce a single, possibly multisample, output dataset." />
+        <param name="group_by_id" type="boolean" truevalue="-i" falsevalue="" checked="false" 
+        label="group reads based on read group id only" 
+        help="If selected, this option ensures that only the read group id (but not the sample name) is considered in grouping reads in the input file(s). If turned off, read groups with identical sample names are automatically pooled and analyzed together even if they come from different NGS runs." />
+    	<section name="adv_settings" title="More options" expanded="False">
+            <param name="md5_check" type="boolean" truevalue="" falsevalue="-x" checked="true" 
+            label="md5 sum verification of contigs/chromosomes" 
+            help="leave turned on to avoid accidental variant calling against a wrong reference genome version (see the tool help below)." />
+            <param name="max_depth" type="integer" value="250" min="0"
+            label="average sample depth cap limit (default: 250)" 
+            help="only relevant for very large sample numbers and/or very high sample coverage; increase to use more of the data, decrease to save memory"/>
+        </section>
+    </inputs>
+
+    <outputs>
+        <data name="ofile" format="bcf" 
+        label="Variant Calls from MiModd Variant Calling on ${on_string}">
+            <actions>
+                <conditional name="reference.source">
+                    <when value="cached">
+                        <action type="metadata" name="dbkey">
+                            <option type="from_data_table" name="all_fasta" column="1" offset="0">
+                                <filter type="param_value" ref="reference.genome" column="0" />
+                            </option>
+                        </action>
+                    </when>
+                </conditional>
+            </actions>
+        </data>
+    </outputs>
+
+    <tests>
+        <test>
+            <conditional name="reference">
+                <param name="source" value="history" />
+                <param name="genome" value="a.fa" />
+            </conditional>
+            <param name="list_input" value="a.bam" />
+            <expand macro="test_mentions_samples" />
+        </test>
+        <test>
+            <conditional name="reference">
+                <param name="source" value="history" />
+                <param name="genome" value="a.fa" />
+            </conditional>
+            <param name="list_input" value="a_part1.bam,a_part2.bam" />
+            <expand macro="test_mentions_samples" />
+        </test>
+        <test>
+            <conditional name="reference">
+                <param name="source" value="history" />
+                <param name="genome" value="a.fa" />
+            </conditional>
+            <param name="list_input" value="a.bam" />
+            <param name="group_by_id" value="true" />
+            <section name="adv_settings">
+                <param name="md5_check" value="false" />
+                <param name="max_depth" value="1000" />
+            </section>
+            <assert_command>
+                <has_text text="-i" />
+                <has_text text="-x" />
+                <has_text text="--max-depth 1000" />
+            </assert_command>
+        </test>
+    </tests>
+    <help><![CDATA[
+.. class:: infomark
+
+   **What it does**
+
+The tool transforms the read-centered information in the aligned reads input
+datasets into position-centered information including variant call statistics
+(using samtools mpileup and bcftools internally).
+
+**It produces a BCF file that serves as the basis for all further variant
+analyses with MiModD**.
+
+-----
+
+**Notes on Advanced Settings:**
+
+**MD5 checksums**
+
+By default, the tool will check whether the input BAM dataset(s) provide(s) MD5
+checksums for the reference genome contig/chromosome sequences used during read
+alignment (e.g., the *MiModD Read Alignment* tool stores these in the BAM file
+header). If it finds MD5 sums for all sequences, it will compare them to the
+checksums of the reference genome sequences used in the current tool run and
+abort with an error message if there is a discrepancy between them. If it finds
+contigs/chromosomes with matching checksum, but different names in the aligned
+reads dataset(s) and the reference genome dataset, it will use the name from
+the reference genome in its output.
+
+This behavior has two benefits:
+
+1) It protects from accidental variant calling against a wrong reference genome
+(*i.e.*, a different one than that used during the alignment step), which would
+result in wrong calls. This is the primary reason why we recommend to leave the
+check activated.
+
+2) It provides an opportunity to change sequence names between aligned reads
+files and variant call files by providing a reference genome file with altered
+sequence names (but identical sequence data).
+
+Since there may be rare cases where you *really* want to align against a
+reference genome with different checksums (e.g., you may have edited the
+reference sequence based on the alignment results), the check can be turned
+off, but only do this if you know *exactly* why.
+
+
+**Average sample depth cap limit**
+
+For each of a total of ``M`` BAM input datasets, the tool will only pile up a
+maximum number of reads ``N`` per position to avoid excessive memory usage with
+very large numbers of samples sequenced at high coverage.
+N will be calculated as the maximum of ``8000/M`` and ``DEPTH*S``, where ``S``
+is the maximum number of samples found in a single input dataset and ``DEPTH``
+is the *average sample depth cap limit* specified in the tool form.
+
+This parameter, thus sets the average depth of the pile-up per sample that is
+guaranteed to be used even when there is a very large number of samples. As can
+be seen from the formula above, however, it will rarely become relevant for any
+regular-size analysis.
+
+
+@HELP_FOOTER@
+    ]]></help>
+    <expand macro="citations" />
+</tool>
diff -r 000000000000 -r f0f2795de2c7 test-data/a.bam
Binary file test-data/a.bam has changed
diff -r 000000000000 -r f0f2795de2c7 test-data/a.bcf
Binary file test-data/a.bcf has changed
diff -r 000000000000 -r f0f2795de2c7 test-data/a.fa
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/a.fa	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,3289 @@
+>chrI
+gcctaagcctaagcctaagcctaagcctaagcctaagcctaagcctaagc
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+tttttgtttactttaggtgggttgacaggtgttgtattatctaattcaag
+attggatattattttacatgatacttattatgtagttagacattttcatt
+atgttttaagtttaggagctgtttttgggattttcacgggtgttacacta
+tgatgaagatttattacagggtatgtgttagataaacttatgatatctgc
+agtatttattttattatttattggggtaaatttaacatttttcccgctac
+attttgcaggactacacgggttcccacgtaaatatttagattaccctgat
+gtttattcggtatgaaatattattgcctcttatggttctattattagaac
+tgcaggactattcttatttatttatgtattattagagtctttctttagtt
+atcgtttagtaattagagattattattctaatagaagacctgagtattgt
+atgagtaattatgtatttggtcacagttatcagtctgagatttattttag
+aactactagattaaaaaattagaaaccttagtataattttagtatattta
+attgcaaattaaatggtattggtttttagtaagataggataattaagtct
+gtaaggttcatacccttgaggtggttttctcttattaaaagttttagtat
+aaaaaagtatattttattgtcaataaaaaggtaaaaactttagattcttt
+agtataattcagtatgtttgacttccaatcaaaaggttttgaggattaat
+taataatttttttcaaggatataatttactatttcagcatagtttatttg
+ctagttatatagattgatttcataggtttaattgtagtttattgttggga
+gttttagtgtttgttactttattgtttggatatttaatttttggtacttt
+ttattttaaaagaaaaaaaattgagtatcagtttggtgaattattgtgta
+gtatttttccaactattattttattaatacaaatagttccttcacttagg
+cttttatattattacggattaataaatttagatagaaatttaacagttaa
+ggttacaggacatcagtgatattgaagatatgaatatagagatattcctg
+ggttagaatttgactcttatataaaatcactagatcaattaagtttaggt
+gaaccacgtttattagaagttgataatcgttgtgttattccttgtgatac
+taacattcgtttttgtattacatctgctgatgttattcatgcttgggcat
+taaattctttatctgtaaaattagatgctataagaggaattttaagaaca
+tttagttacaggtttcctatagtgggagttttttatggtcaatgttcaga
+gatttgtggagcaaatcatagttttataccaattgctttagaggtaacat
+tattggataattttaaaagatgatgttttggtactatagaataatttagc
+ttaatagtttatattaaaatgtttacttgtggtgtaagagaatatagagc
+tttaaattttacttgtttaaatattggtattgcatactattacaataaaa
+tttcatgttaatgaaaaatagaaacaaagggtagagtaaatattagtttt
+attgtttcatactaaaaattatatttattagagttgatatgtcgaccttt
+gtgataactgtttttatttttatattagaaaattatatattatataatta
+ttttaggaaatttaaaatttgaagtgttttaaatttatgttttacaacat
+tttcctaattttatttaagtttaatttttaatttaataaagttttattaa
+ataaataatttgtaaattagtaaattttataaatttaatttattattaaa
+atataattgaagaacttgaagtcttgatcaaatgttttttaaagacttag
+gctttatattaaagctggcttctgccctatgatatttaaatggcagtctt
+agcgtgaggacattaaggtagcaaaataatttgtgcttttattgagttcc
+agtatgaatgaagttattggttagttctatttatgttttatgtttgaatt
+taatttttatttaagaaaaaataaatatatttatacaaagataagtcttc
+ggaaattctgttattacacaattaaataattgtgtaataaattttctagg
+gcagaatattatataatagtatttcactatatttaatttaaagaattact
+ccggagttaacagaaaatcatacctaatctagtacttatagtaaggtaag
+ttttacatcgatgttgtattcagataatctaagagaggagaaggcttagt
+agtttagactgttcttctattaattaatctgacgtgatattagtttaatt
+cattgtgagatagaattgtttatcttgataaatatttatatttaatacat
+ttagtacgaaaggaacattgtaaaagttttaaactttaaagattttgaaa
+tctttattttagtgctattaatagttttagtgtttacgctagttttatta
+tttgctttttatttgattaattttttattaagaattaaggatataggaaa
+aaataaaattagagcgtttgaatgtggttttgtaagagttggaaaaattc
+aaaattcttttagaattcatttttttattatgatattgatatttgttatt
+tttgatttagaaattgttatgtttttaggtattttagtatcagatttaag
+ttcgtatatcaggtttttaataatattcatcttcatcttgggaggatttt
+acatagagtgatgatatggtaaattagtttgagtaatttaattaatattt
+ctatttttttgattggatttgttttttttataggtggaattagtgtttgg
+cttatacccacatttaaattaggaatcttttttttagaatgagatttttt
+aaggttaaaatttaatttttattttaatagaatcttattttcgtttattc
+tttttttggtaacgtttagagttttagtttttagtacttattatttaaat
+agtgagttaaactttaattattattattttgtattgttaattttcgtagg
+tagaatgtttaggctaaattttagaaacagtatttttacaatgttactaa
+gatgagatttattgggtatttctaggttttttttagttttattttataat
+aattgagatagatgtaggggtgcaataaatacagcattaactaatcgtct
+aggtgattattttatatttgtcttttttggtttatcggtttttagaggtt
+attattttttaagatttagaatatttagaagttatatatctttattatta
+cttttaacagcttttactaaaagagcacaatttccatttagatcttggtt
+acccaaagctataagagcccccacaccggtgaggtctttggttcatagta
+gaactttagttacagcaggattaattctattaataaattttaataattta
+gtaatacagaaagattttatcagttttgttctgattattggcctatttac
+tatatttttttctagcttagcaagtttggtcgaagaagatttgaagaagg
+tggtagccttgaggacactttcacaaataggtttttcaatagttactttg
+ggcctagggcttaggtttatttcatttattcatttagttagacatgcttt
+gttcaaaagatgtttatttatacaagtaggttacattattcattgttcat
+ttggacaacaagatggacgtaattataggaataatggtaatttaccaaat
+tttattcagttacaaatattagtaaccctattttgtttatgtggattaat
+tttctcaaggggtgctgtaagaaaagattttattttagaattattttttt
+ctaataactatataatgttttttagattaatattttttgtttcagtgttt
+ttgacttttggttacagttttcgtctttgaaaaagattttttttaaggtt
+taataaagtaataaatcattacagtagcacagtatttataaattttttaa
+gtttagtattagttattttttctattagatttttatgatgaataaatttt
+aatcttcttaacatcccaagacttttcttatacgtagatttttttggccc
+tctagtatttttatttataataatttttttatcttttttaattttaaaaa
+tattatttaaggagttaatatacaagtttttagttgattatttggctaaa
+aatagtatttataaaataaagaatttaaaatttatagatttatttttaaa
+taatattaattctaaggggtacaccttatttttaagcagtggtatattta
+aaaattactatttaaaaaggttaaattttaatagtgtagtagttttaatt
+tttattttttttataatttgttaagggattttagtttaataaaaatatat
+gttttgcatacataagataataattctagatagttttacacgcgcgtata
+cgcgcgttataaatatatatatatatatatatatatatatatatatataa
+taataatattatatttatattataaatataatatttattataaattatat
+attatatttatattataaatataatatttattataaattatatattatat
+ttatattataaatataatatttattataaattatatattatatttatatt
+ataaatataatatttattataaattatatattatatttatattataaata
+taatatttattatatattatatatattatatttatattataaatataata
+tttattattaataattcatcatatttatattataaatatgatgaagtact
+aaaaaaaagatgaatattctataatatatttagatatattatagagtatt
+tatcttattatttatagatatatactttgtatatatctatatta
diff -r 000000000000 -r f0f2795de2c7 test-data/a.sam
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/a.sam	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,824 @@
+@HD	VN:1.5	SO:coordinate
+@RG	ID:000	SM:N2
+@RG	ID:266-1	SM:ot266	DS:mutant strain carrying the vab-3 allele ot266	CN:Columbia University	PL:Illumina
+@SQ	SN:chrI	LN:25050	M5:7d9f340160ce45568f9bf48d8e16dcf4
+@SQ	SN:chrII	LN:25050	M5:ba5a89bf12f55a4bd88ddf14e6d27bd6
+@SQ	SN:chrIII	LN:25050	M5:ba78739f84b6c850448bec286bdd8798
+@SQ	SN:chrIV	LN:25050	M5:0807d4df5c3593363a5b015f19ef81c2
+@SQ	SN:chrV	LN:25050	M5:cf942c1556ef72fd9ba352e4a7d9cf9e
+@SQ	SN:chrX	LN:25050	M5:5ce1b12d74140cfc288b493c16fe280b
+@SQ	SN:MtDNA	LN:13794	M5:199e147d502d88e45047413dc83c039c
+@PG	ID:SNAP	PN:MiModD snap	VN:0.1.8.0	CL:paired /var/tmp/batch_dataset_548966_dataset_35_20_0.3 /var/tmp/batch_dataset_54896612531.sam -d 8 -S 200 -n 25 -a 7 -F s -o /var/tmp/batch_dataset_54896615435.tmp -h 250 -c 2 -C++ -M -s 100 10000 -t 2
+BS-DSFCONTROL03:121:D0M8KACXX:5:1314:19049:65217	99	chrI	556	60	100M	=	702	246	CCTATATGCTCCTGTGTTTAGGCCTAATACTAAGCCTAAGCCTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAATACTAAGCCTAAGCCTAAG	CCCFFFFDHHHHHJJJJJJJIJJJJJJIIIIIJIJJJJJGIIJIIHIJIJJHIJIJJHIJJIIJJJIIIIGGGIJHHHHHFFEFFFEECEEDDCACDDC?	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:56:17087:17617/1	0	chrI	575	60	101M	*	0	0	AGGCCTAATACTAAGCCTAAGCCTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAAGACTAAGCC	GGFGGGDGFGEFBGFGFGGGGGGFGGEGGGGGGGGGGGGGGGGGEGGFFEFGGFFGEAFGFDEGGGGGGGEEFDDEEEEEGFGGEGEDBEEAEDEECFEBC	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1314:19049:65217	147	chrI	702	60	100M	=	556	-246	AGCCTAAGACTAAGCCTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAATACTAAGCCTAAGCCTAAGACTAAGCCTAAGACTAAGCCTAAGAC	CCC>AA>;(CCC;C@3HEEEGCIGED=EGGGJHGJIGGCIJIIDFGGIGDIHJIHEGIHJIIEGGIIGJJJIGIGJIJJJIGDIHGIHHFHGFFFFFCB@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:81:6795:13435/1	16	chrI	842	60	101M	*	0	0	TTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAGTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATA	A:@EEEEEBEEEEEEEEEGEEEEGEEEEEEEED=BFEEEGEEGEGFGGGEEGGGGGGGGFGGGFDDAGG=GGEGGGFFFEFEECBFDGGEGDGDGGGFGEG	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+EAGLE:1:46:9721:15037/1	16	chrI	842	60	101M	*	0	0	TTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATA	AEEBEBBEEEAAEFEFFFE?FCCCBC?DDBD5DD=D:DDC:CC?C>6EB@CEEEDDEECEEEEE8BFFEEFEEADDCCC@CBCCEEFDFFAEFGFDFGGGD	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:77:15674:6412/1	0	chrI	844	60	101M	*	0	0	GAAATGAATATCGTAGCTACAGAAACGGCAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAACATA	GFFGGGCGDFGEGDFEEFEGGFEGFEEEACC?BCCDDCDECBECDEFEECEEDEEEEEEEEBEEEEEEAEEEEE=AC:=CBBCCCC@::?CACC@BBBABE	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:88:10221:3318/1	16	chrI	845	60	101M	*	0	0	AAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCATATATAG	AEBABBEEBGEGEBGGGEEEEGEEGGEEGGCEEEFEFEEBGGFDEGAEFGGEDFF=EEGEGGGGGG?DBDFGGFGGDFECCFDDCGEEEE:AG?GFFGDGG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:87:4119:14575/1	16	chrI	845	60	101M	*	0	0	AAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAA	GEEGEDEEGEGGEAEFEB?DEEDBGGBGGFGEFBGFGGBGGGGGFGGEGFGGGGGGGGGGGGFEFAGGGGEGGDGGGGDFGGBFGGGGDFDGGDGGGGGEG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:95:9634:15406/1	16	chrI	845	60	101M	*	0	0	AAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAA	FEEE?GEFEBE:ECE=BEGGGEGEGBFFFEFAFDFFGEBBGGGFGDGEGGGEEEEE5FFFFCFGAEGGEGGGGGGGFGDGGGFGGGGGGGFGDFFFGGGFD	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+BS-DSFCONTROL03:121:D0M8KACXX:5:2113:3662:39793	73	chrI	846	60	100M	=	846	0	AATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAA	CCCFFFFFHHHHHJJJJIIIIJIJJJFGIGIIJJIGIJIJJGEHIHJHEHHHJIIJJJJJIJJIGIJIHHHHHHEFFFFECEEDBDDCCDDDDD?CDDDC	PG:Z:SNAP	RG:Z:000	NM:i:5
+BS-DSFCONTROL03:121:D0M8KACXX:5:2113:3662:39793	133	chrI	846	0	*	=	846	0	GTGTACTACCGTTTCTGGAGCTACGATTTATTTTTCAAAACACGCAAAAAGTGTTTTTCACTATGTACAAAATCATCTCAAAATCGGAAATATTTTTTGT	<<<@@@@@@@@@@???####################################################################################	PG:Z:SNAP	RG:Z:000
+EAGLE:2:45:11395:10768/1	0	chrI	846	60	40M3I58M	*	0	0	AATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCTAAATAT	G?FEGGBGGEFDFDDEDEBEEDFFEC@D=AFFFCFFFFFEAFEDFFEF=FFFFFFFD>DF?FFAFFFFFECCAFE=BEAEEFBDEEFEEEEEEEEBEFDDA	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:1:47:11205:5807/1	16	chrI	846	60	101M	*	0	0	AATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAGTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAG	FEEDBBFEAEFDEFEE?EDFEEACFEDAFDDFEFFFFFFFFFFFFEEBFFEFEFFFFFFFEEFFFFEEFFFFFFFEEACAFDGGFGGGGGGGGGGGGDGEG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:69:6499:16127/1	0	chrI	847	60	101M	*	0	0	ATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGC	GGGGGGGGGEGGGGGGGGGGGFGGGDGGEGGGGGGGGGGGFGGGGGGDFGGDGEGGFGG=EFFFFAG;CGGEBGEEFEFGGGGF?CEBCFFEBFEEEEF?F	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:24:6173:14843/1	0	chrI	847	60	101M	*	0	0	ATGAGTATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGC	FDGFGGDEGEEG?GGAEEEEBEE?::AB;AADB=CEFFFD=EDFF?EBEEEC@@EFDEEDADEDFE;EAFD::D:BCB<BEEDB8=DB??BBB5:=@:@@:	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:52:5204:4050/1	0	chrI	847	60	101M	*	0	0	ATGAGTATCATAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGC	FFFFEFFFFAEAEAEGGGGDEGGGGBFEDEFFFFFGGGGF=FDFFGGGGGGGAEGGGGGFFFGFGGGAGEEGFG?GFDEEGFFGBEEEEEFEDGFDG5FFB	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:1:88:14674:10686/1	16	chrI	847	60	101M	*	0	0	ATGAGTATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGC	EEEEAEBBEDAEEEEEFDFDDEGEGGEDFBEBFEFEBGCEGCEE?8DEFDEFGGGEEEEC>?FEGGFEGGGCEEBE:CCEEEDFGG=EFBGGEBFGGGDDG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:29:17996:20445/1	16	chrI	847	60	101M	*	0	0	ATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGC	EE5?EEBBBB?E=DEDFEFEBADCEECBAAE=E=EBDF?EGEE=GCDFFEDBDFGGDGGGGEF=GGGGGBDGGGDGBGGFGGGGGGGFEGGGEEGEEGGFG	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:112:10138:4083/1	16	chrI	847	60	101M	*	0	0	ATGAGTATCATAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGC	EC?@BEACB:C@=EE?CAECC@CCCE>=A<55CCD?BEE?E?FFA;GGGGGEGEGEEDBGGGGEGBGGEGGBFGDGCCCGEG?GDGDFFGGFFFFFE?EDE	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:54:15434:16430/1	16	chrI	847	60	101M	*	0	0	ATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGC	EEEE@EEEEEEDGEEEGEGDEEGGGGEG?DFEGFGGFFGGGFGGEEFGGGGGGGGGGGGGGGGEGGGGGGGFGGFGBGDGGGEGGGEGGGEGGGFDDGGGF	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:58:7649:14576/1	0	chrI	848	60	101M	*	0	0	TGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAGTTGCAATTTTTATAGCTAAGACACTTTTTGTCTGCCCAAATATAAGCA	GGGGGGGGGGGGGGGGGGFGGGGGEFFEDFEGGGGGGGGFGGGGGGCFFGGGGEFEGGFGGGGGFGGGGGFFFGGGGEGGGGEEEGGGGGFEGEF?FBBFG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:76:11197:2138/1	0	chrI	848	60	101M	*	0	0	TGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCA	EEDFFFEFBDADEEEFFBFFFEFFE=C@?CFFFFFFFDF?EEEDEFBDFFFFFFFFFFAEAEDEBC;FFEFEFFDDABFFFFE;CFEFEEBEFBDBDDDEB	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:1:59:14370:7837/1	0	chrI	849	60	101M	*	0	0	GAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAAAACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCATATATAGGCAA	FGFGEGGEBGFGFGGDGFFGEED=EA@=DDFFE?FEFFFFFFFFFEEEEEFEEADFAFFFFFFFEEFEEBEEBEFEDEEEFEEEEBEEBE:EBBBB@BBAD	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:79:15239:5534/1	16	chrI	849	60	101M	*	0	0	GAATATCGTAGCTACAGAAACGATAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGGCACTTTTTGTCTGCACAAATATAGGCAA	BFDGDDFFEDEGGDGFEEFBEFFFFEECECEGAEEGGGGFGGGEEGGGEGGBFGGGGGFGGFGGGFGGDEGGGGEGGFGGGGGEFGEGFGDFGFGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:83:6103:1248/1	16	chrI	849	60	101M	*	0	0	GAGTATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAA	@>B:9?BBBBDBB?DBEBEEEBDDDDD?FAEEEEEEEEEEEEEEFFEFFFEDFEFEECCDAFFFFFFFFFFEBE?@EDFFEFFEFFFFFFFFF?DFEFFDD	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:60:3721:11022/1	0	chrI	851	60	35M3I63M	*	0	0	ATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCA	DGBGGGFGFFE=EEEDEFFFEBEEEED=EDFDFDFFFFE:FEFEDFDFFFEFFFFFFEDFFFFEFCAFFBBEEBEFFEEEFEAEEEEEEFEFFEEEEBEEF	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:56:9356:7127/1	0	chrI	851	60	101M	*	0	0	GTATCATAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACC	GFGGGGGGDGGFGCFDBFFFGDGGDGEFFGGDFEEFGDGGEGGGFGGEEEG?GFGEEEFEED;@EGEBEEEEE?BDDDECEEEEEGEE?EA?AC@CCA5??	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:26:13486:6669/1	16	chrI	851	60	101M	*	0	0	ATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACC	GE:EEEDDDDAEGEBEEEFEGEEDABFCDEEEEEGGGEGGEEGGFGEDEEFAGDGEGBGFFGGGEGEGGGDFACCFFFGGDGGGEEBGGGEEEEEEEE?EE	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:90:7286:17726/1	0	chrI	852	60	101M	*	0	0	TATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCA	GGGGFGGGGGGGGGFEGFFGFDDBGGFGGGEFGGGDAGEGGGFDGGDGGGGGGGE?GGGGGGEGGGGDFGGGGEEFBCEEDGFFBGGDGDGGEDBEEGDEB	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:60:11520:19697/1	0	chrI	852	60	101M	*	0	0	TATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCA	DEDEEFFFFFEGGGFFFFFFBEE?EEEEEEFBFFFFFFF?FFFFFCFFCFFDFFAEFFFFEEADFDDF?EFFFEFFFBEEEEFEFFEFF@FDED:BEEBEE	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+BS-DSFCONTROL03:121:D0M8KACXX:5:1102:21095:33794	73	chrI	853	60	100M	=	853	0	ATCGTAGCTACAGAAACGGTAATACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGGCACTTTTTGTCTGCCCAAATATAGGCAACCA	@@@FDFFFHHFHHJJJIJJIJJJJJJJIIJGGIIGGIIII4?GIJJIIJIJJJJGIJJGGIIJJJJJDEHGEFCDCEDEEEEDCDDBDDDFEDCDDDDDB	PG:Z:SNAP	RG:Z:000	NM:i:2
+BS-DSFCONTROL03:121:D0M8KACXX:5:1102:21095:33794	133	chrI	853	0	*	=	853	0	GCTTCACAGTATATTTTACTTTCTGTGGCTTCACAGTATATTTTACTCTCTGTGGCTTCACAGTATATTTTACTCTCTGTGGCTTCACAGTATATTTTAC	A>;6;6(CA;;@B?3DB=AA7EGGIJIIGGIGEIJJJJJHEGGCBFGGFBBFG@IGDJIIJJIIJJIJJIGHIGHGJGHGIHCJJJIHHHHHFFFFFCCB	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:1304:18505:45478	73	chrI	853	60	33M3I64M	=	853	0	ATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAA	@CCFFFFFHHHHHIIIIJJHDHCHIIHIIJIJGIEGGHEDFFGEGIIJIJJJJGIJGGGIJIGEGGGJIHDHEH;?CDFFDECECAA?>BBDDDE>:CCC	PG:Z:SNAP	RG:Z:000	NM:i:6
+BS-DSFCONTROL03:121:D0M8KACXX:5:1304:18505:45478	133	chrI	853	0	*	=	853	0	GGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTC	?;;5::>CDED@DEFDDDDFFFFFFHHHHHJIIIEJJIJJJJJJJJIIHFIGGIIGJJIGJIJJJJJIJJJIJJIJJJJIJJIIIIIHFFAHFFFFFCCB	PG:Z:SNAP	RG:Z:000
+EAGLE:2:96:9394:5489/1	16	chrI	854	60	101M	*	0	0	TCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAA	EGEBEEEEEFEGEBEGEEE:E?DFCFFFGEGGFGGGFGEFGGGFGEEEGGGGEGGGGFGGGGGDGGGGEEGGGGGFGGGGGGFGGGGGGDGGGGG?GGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:1:110:3453:13829/1	16	chrI	855	60	101M	*	0	0	CGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAA	EAEAE?5EEEEC5=EBDFFDFDGEGEGGDBEFGGBFAEEGGGGGGEEEEDEE8FFFGGGGFGEGGGFFEEEDG?GGGDGGGGGGGFGAGDEGGGAFGEGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:1:34:16346:19478/1	16	chrI	856	60	101M	*	0	0	GTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAA	DDD?BB@B=BBB>DDC@@B?:A?:AAEEDEABCCB6EEEEEBEEDDED:DC>EEEEBDDCBD5CEBED=CEEBEEEEEEDEAEEAEEFGGFBEBEAEEDEE	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:1:75:1484:17855/1	0	chrI	857	60	101M	*	0	0	TAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAGTTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAAT	DD@CADEEEEEEFFDBDDCDEEDEEAFDEFAFFFFDFFFFFFD=FDFFFFFFFEF;CCEEDDDFDBEAAFFFFF8EBDEDDDFFAFDFFBFFFEF5FFEF=	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:110:6822:2912/1	16	chrI	857	60	43M1I44M1I2M1D10M	*	0	0	TAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAAATTGCAATTTTGATAGCTAGGACACTTTTTGTCTGCACAAATATGAGCAACCAAAAA	DBFBDCECFEDEFGFGEGFEGEGGGGEFFGGEGF=BGGGGGGGGGFGGGGGGFGFGGGGGGGGGGEGCGGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+BS-DSFCONTROL03:121:D0M8KACXX:5:1210:12858:84933	83	chrI	858	60	100M	=	876	118	AGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAGTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAAT	CCCEEDDDDBDBCDEDDEDCDDDDDDDCEEEEEEEDFHHHHHJIJIJJIHJJJJJJJJJJJJIGGBJJJIJJJJJJIIJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:4
+EAGLE:2:91:13868:11992/1	0	chrI	859	60	41M1I59M	*	0	0	GCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAAAATTGCAATTTTCATAGCTAGGACACTTTTTGTCTGCACAAATATAGGCAACCAAAAATA	GGGEGGGBEDGGECEEDFFFGGGGGGGGFGGGEGGGGFFGGGGAG=FFBDFFGGGEAFGBGEGBGGAEGEGFEEEEDEEEDFF=ECBCCC?AECCCDDECE	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:119:12921:6612/1	16	chrI	859	60	27M3I71M	*	0	0	GCTACAGAAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAA	BBEEGEE=FBEEFDFGFAGBGGGFEGEFGDGEEGGAEGGGDGGGGDDFGDGGEEGEGGGEGEEEEBEBCCEFDGFFGDGGEFFGGFEEEEEFFBFFGGFGF	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:111:6913:8666/1	0	chrI	860	60	101M	*	0	0	CTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAAT	GGGGGGFFFGFF@EEAEEEEBGGGFGGEGGGGEGGGDGEDCFA5F?EEEEGGG>GGBFEEFFEGGEFFAFF>EDEC?FEDABBBBD=A:D=B=EB@E@?;>	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+BS-DSFCONTROL03:121:D0M8KACXX:5:2216:18829:62698	83	chrI	860	60	100M	=	871	111	CTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAA	CEDDDC?BBBCDEEEECC@EDEEECEFFFFEFHHHJJJJJIJIJIJJJJJJJJJIJJJJJIGGGJJJJJJJJJJJJJIIJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:3
+EAGLE:1:67:7435:14824/1	16	chrI	861	60	101M	*	0	0	TACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCATATATAGGCAACCAAAAATAATT	::GEEGEEGDGGGEGEGDDBGG?GGGGFGGG@FFAFEGGFFEFGDGEFGGGFFGGGFFFFFGBFGGFGDGGGGGGGGGEFGGBGGGGFFGGGFGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:2
+EAGLE:2:11:15002:3991/1	16	chrI	862	60	101M	*	0	0	ACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTT	DEEFEDBEFEFDFBDF?EEFFDG?GEFEFC=EGAFGEGDFDFGGEEG=?GGGDDDFCEEBE6@@>A=GFFGGCFGGEEEEE:E?EEAEDEECEEE?5EEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:96:4105:9079/1	0	chrI	863	60	101M	*	0	0	CAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTC	GGGGDGGGGEGGEGGDGGGGGGGDGGGGGFGGGEGGFEBGDGGEGEG?GEFEEGEEEEEGDDEDEFEBEECC=DFDDDCCEAEBGEECGE@DEF???;?BE	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:1204:8976:92778	73	chrI	864	60	100M	=	864	0	AGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTC	BCCFFFFFHHGHHJJJJJJJJJJJJJHHGHHJJJJJJJJJJIIJJJJJJJIIJJJIJJJJGIJJJJJHHHHHHFFFFFEEEEDEDDDDDDDBBDDDEDEC	PG:Z:SNAP	RG:Z:000	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:1204:8976:92778	133	chrI	864	0	*	=	864	0	AGAGTGTACTACCGTTTCTGTAGCTATGATACTCATTTCAAAAAACCCGAAAAATGATTTTTTCAATATGCAACAAATCATCAAAAACTTGGAAATTATT	BCCFDFDFHHHHHJHJIIJJJJJJJJJJJFJJJJJJJJJJJJJIJJJJJJJJJJJIJJJJIJHFFFFFFECDCEEBDDDDDDDDDDDDDDDDDDDDDDED	PG:Z:SNAP	RG:Z:000
+EAGLE:2:89:4630:12148/1	0	chrI	865	60	101M	*	0	0	GAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCA	GFEGGFFFFFB=DDCGGGGGEEFFFDFFFFGGDGGDGG=EDCGGGGGEG>GGFGFBFFFBGEGGGDCC=FEEEEEEE=E:EBEEEFGF?GGCAA8;EEBE:	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:71:6779:3992/1	0	chrI	866	60	20M3I78M	*	0	0	AAACGGTAGTACACTCTTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTC	GGGGEFGGFFEGGFEGGGGGBGFEGFGGFEGGFGEACCFFDGEBFGGGGFGEGGADBFEFGGEE5EEE=ABBFEGGGGBFEE=EEEDGGBEEE=E@B;CAE	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:90:8794:13563/1	0	chrI	867	60	101M	*	0	0	AACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAG	FFFFFEDFFFGFFFGGGGFFEGGGFFGGGGFGDGEFFGGAGGDGFGGGGFFCGGDGGGBGDF:F;ACEEEFF:AEDEEAEEDECGEGFGGE8C;DD=BD?B	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2310:12542:49797	73	chrI	869	60	100M	=	869	0	CAGTAGTACACTCTTCTCAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGT	BCCDFFFFHHGHFJJGGJJJJJHJIJIJJJIIJIJGIJJJJJJJJJIIIJJJIGHHGGGIGEHGIGEHEGHFGGHCCFBDFFEDDDDDDDDEFEDEDCCD	PG:Z:SNAP	RG:Z:000	NM:i:5
+BS-DSFCONTROL03:121:D0M8KACXX:5:2310:12542:49797	133	chrI	869	0	*	=	869	0	GTGTCCTAGCTATAAAAATTGCAAATTTTTTGTATTTTCAGAAGAGTGTACTACCGTTTCTGTAGCTACGATATTCATTTCAAAAAACCCGAAAAATGTT	CB@DFFFFHHGHHJJJJIGGIJJIJIGIIJJJHIIIJIIJJHHIIIGHHIIJJJJJJJJJJJIJJJJJJJIHHGHFFFFFFFEEEDDDDDDDDDDDDDBD	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2216:18829:62698	163	chrI	871	60	100M	=	860	-111	GTAGTACACTCTTCTCAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTT	CBCFDFFFHHHHHJJJJJJIJJIIJJJJJJJJJJJJJJJJJJJJJJIJJIDHGHIIJJJJJJJIJJJEIIEEHHHHGFFFFDDDDDDDDDE<@DC5@@>>	PG:Z:SNAP	RG:Z:000	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2306:15570:52084	73	chrI	871	60	100M	=	871	0	GTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTT	@@CFDDFFHHHHHJJJJJJJIJIJJIIJIJJJJJJJJJJJJJJJJJJJJJIIJGIJJJJHHFHHHFFFFEEEFEDCCCDBDDDDDDDDDEEEDEECDDDC	PG:Z:SNAP	RG:Z:000	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2306:15570:52084	133	chrI	871	0	*	=	871	0	TTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTT	BBDDDDDDDBBCECEEEEFFFFFFHHHJJJJJJJJJJJJIIIJJJIIIGJJJJJGJJJJJJIHFJJJIGJJJJJJJJJJJIJJJJJJHHHFHFDDFFCCB	PG:Z:SNAP	RG:Z:000
+EAGLE:2:85:11800:16523/1	16	chrI	871	60	101M	*	0	0	GTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTT	GEGGEEFEGEGEEGEGGFGGE@GGGGGGGEGGGFGEGEGEGGGGGGGFGGEGEGEGGFGGGGFGGGAGGGGGGGGGGGGGGGGGGGGGGFGGGEGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:50:15754:14085/1	0	chrI	872	60	101M	*	0	0	TAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTT	FFFFFFFFDFFAFFFFBDFFFFFFFFFFFFFFFFBFFFFFFFEFFFFFFFFFFEFFFFFEEFFFFFFFFFEFFBFEFFEFFF?FFDEEEEFFEEEF@DFEA	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:51:4256:7814/1	0	chrI	873	60	101M	*	0	0	AGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATTATTTCCAAGTTTTTG	EFFDGGGGGEGGGFAFFGGGGGGFFGGGGFGGF?DGFGGGGGFGGFFAFGDGEDEGGGEGEFGGGEGGGDDFGGFGGEGBGGEFBGFEEEEEEBEEEEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:83:2757:15592/1	0	chrI	874	60	101M	*	0	0	GTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGA	FEFFFFDFFEGBGGGGFDGGGEFFEDGGDGFBEAFFFFEACDGDEEDGGDEGEGGGGEEDEEEDDAE?EDBEEEBEEEBBGEEE@@@@BDD@?B@@BBB@;	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:74:11870:7997/1	0	chrI	874	60	101M	*	0	0	GTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGA	GGEGGGGGGGGGDFGGGGFGGGGGGFDFFGGGGDFGGGFGGGGFGGGFGGGFEGGDB@GEGEGGGBGEFGEEGEEGEGEEBFEDCEEEE?EC@FACFDFDC	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+BS-DSFCONTROL03:121:D0M8KACXX:5:1210:12858:84933	163	chrI	876	60	100M	=	858	-118	ACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGAT	CCCFFFFFHHHHHJIHJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJHHHHHDFFFDDEEEEEEDEEDDDDEDDDBC	PG:Z:SNAP	RG:Z:000	NM:i:4
+EAGLE:1:89:11320:10564/1	16	chrI	876	60	101M	*	0	0	ACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATG	@EEFEGFEGFEG=GDFEGGEGGEGEGFBGGGEGFGFGGGGGGGGFDFEGGGGGDGAFGGFFGFGGGGFGGGDGGGBFGGGGGFGGGGGGGGGGGDGDGGFG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:87:16027:10849/1	16	chrI	880	60	101M	*	0	0	TCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAATTTTTGATGATTT	?BBBB@BBBB?;<BBB?BBBBBBDAB88?:?=BBBBBAADA?B;<<BC?BBBDDDABBADCA=B=?@@=5>@A:C?BC??DBEABDE=EEDDDD??DDDBD	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:98:7014:9919/1	16	chrI	880	60	101M	*	0	0	TCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTGAGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTT	=BGECGFEEGG>?EFEAFEFAEGGABG@DBEFDGDGGFEEGEDCCGGFGDGDEEEDEFGFGDGGGDGGGFGGEGGGGFGGFDGGFGGGGGGEDGGGGGGGF	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:53:7732:11243/1	16	chrI	882	60	101M	*	0	0	TTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGT	E@D=DF@BB:8EFDEEBB==B?FEEEAABBAEFFFDECEEEBB@EDCE?CAE:EAE?AE=EEEE?ED=D<>EDAF?DDFBFAE?EE??BFAEEDEEFEDF?	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:65:9151:20950/1	0	chrI	884	60	101M	*	0	0	CTGAAAATACAAAAAATTTGCAATTTTTATAGCTGAGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTG	GFGGGFGGFGGGEGEGGGGGGFEGGGEGGDGGFGG?GGGGGGGGGFGEGGGGGGGGGFGFGFGGFGFGGEFGGGEE@EDFEBBE?EEEEEBBEBDEEEEE?	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:96:16607:9367/1	16	chrI	884	60	101M	*	0	0	CTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTG	GEEGEGFCCFAGD:GEEAGFBGGGFGE=DDE:BFFFDCE5@AAFBDAEFBCCGFFFAFFFEFDDFDDBB@ABF?FDEGAGEFFFFAGGGDGEFAF?DEFFF	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:99:9928:13510/1	16	chrI	884	60	101M	*	0	0	CTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTG	GEFEEGEEEGG?GGGGEBEGEFEEEBEGFGGEGFGFFCFCEEFFFFGFGBGFGEGFGGGGFGGEGGGGDGGFEGGGGGBGGGGGGGGGGGGEGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:1:88:16505:15331/1	16	chrI	885	60	101M	*	0	0	TGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGC	BEFGEDEEGGEEDEEDDEEEDEEGEEGBGGEEEEEEDF?CCGGGGGGFGGFFGDDGGGGGFGGGGGGFFFGGEFDGDEFFDGFFGGGGGGGGGFFGGGDGG	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:78:3200:13969/1	16	chrI	886	60	101M	*	0	0	GAAAATACAAAAAGTTCGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCA	@B:BA88EEEE?=BBB=CEB8>DE?EEFCBFCBCCBE@B@C;DEFFFEFFFBFFDFFFFFFFFFFEEEBFFDEE5EBEEEEEAEE?EEE?DD;DEEFEF=D	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:87:17306:17581/1	16	chrI	886	60	101M	*	0	0	GAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCA	AABE=8;AAA<?AA?:=EDBC@DFFFACCC=BADEEDB@;AFEFFFFDFDFEDAEEBEDEEEDEEEBCEEADFDFFEEE=AFFFFFEEFBBBEFFDEAAD?	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:48:14507:16490/1	0	chrI	887	60	101M	*	0	0	AAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCAT	FFFFAGGGDGGGAGGFDFFFADEDEDAEDDFFB:FEAEEAEABBA::@C@BE?EECE?BB?CDACBAABEE?C=ABBB@B>==A=AAAAAEEEEEBD?BB:	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:54:4351:16632/1	16	chrI	887	60	101M	*	0	0	AAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCATATATAGGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCAT	EGEGAEGGGGGEGGEGGGGEGGGGGGGGFFGGGGGGEGGFFGGGDGFGFGGGFGFGGGGGGGGGGGDDFFF:EGGEGGGEGFFGFGGGGGGGGGGGGGDGG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:115:17415:17791/1	0	chrI	888	60	101M	*	0	0	AAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATA	DD@D7EEEEE>CCEFFFFDFFFFFF8EFFFEFFFFFFBFFBFF:DDFFFFFFDFDFF?E@DEBAEFDFF;C@C?EE=DADEEEE;D:DD@EEE?BE>E=ED	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:9:16465:5809/1	16	chrI	888	60	101M	*	0	0	AAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATA	D?FDGDGGGGFDGGGBB=DGEFEGGGFFDFFEEEECD=GEGGFGGDGGGGG?FGFGGFGGG?GGGGGGGEGGEGFGGGGGFGGDBGFEGGGFFF:FGGGBG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:98:3753:20118/1	16	chrI	889	60	101M	*	0	0	AATACAAAGAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCATATATAGGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATAT	BGBDFGFFFBF=BDFGGEFFGGG?GE?FDEFECEBB>FF=FEDGFDF=EFBGFGGGEEDADE>AAEFDGAGDBGFGDFFFE?FDEEGEEDDFEDFFDADG=	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:33:4809:11289/1	16	chrI	890	60	101M	*	0	0	ATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATT	=EEFEGFFF=BDBBGG=EGGBEDGGG:DBEF?GCFGGDGFGEGEFGGEGGEGGDGGGDFDGCGGGGFFGGGGGGGGGGDFGFFGFGGFGGGGDGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:71:4682:12647/1	0	chrI	891	60	101M	*	0	0	TACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTG	GGGGGEGGGGGDGGGGGGGDGGGGGGGGGGDGGGFGGGEGAGGFGGGFGGFGGFGGGGGGGGFGGGGGGGGGDGFAEA:CEEECEE9CCECABCBDDDCDD	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:75:5406:10078/1	0	chrI	891	60	101M	*	0	0	TACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDEGGGGGGGGGGGGEGFGGDGGGFGGGGGGGGFGGGBGCGFGGEEGGFEGGEEGCFFGFFE?	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:39:9088:2610/1	0	chrI	891	60	101M	*	0	0	TACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTG	ECE??D>A<>EEE:EDEEEEDEBBCE=EEEEE?DEEEEED@B=EADDEEEA;EEEDEEEE=EDD=EECCADDED?BAACDDB6?@?5DDBBEAE=EE=EAA	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:66:8142:4981/1	16	chrI	891	60	81M1D20M	*	0	0	TACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAGGCAACCAAAAATAATTTCCAAGTTTTAATGATTTGTTGCATATTGA	>@DFGGE=EA:FEDFCABBE?DBEE?EEEEEFBCCFADFEGDFF?CEEEEBE?EEEDCAA>GFDEGEADEDFDFDAFBADFGFAEFFFEDEDDDEEFDFFE	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:77:9937:16848/1	16	chrI	895	60	101M	*	0	0	AAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAA	BEEEEEEEEEEEEEEEEEDEGEEDFEAE<CCGFDEEFFGGFGEGGGGDGFGGGGDFFGGGFGGGGGGEGEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:20:3429:5322/1	16	chrI	895	60	101M	*	0	0	AAAAATTTGCAATTGTTTTAGCTAGGACACTTTTGGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAA	ABGEGFEEAEECEFDFDEAFGFGGGGCEDEEEEE:DEFGGFF?FGFFFFFFFFD=FFGGGGGGGFGFGEEDGGEEGFFFFEGGEEEGEEEEECE??CEBEE	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:106:10002:5503/1	16	chrI	896	60	101M	*	0	0	AAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAA	BBEEECEBGFGDGDGFGGAEGEDAB?EBB8DFD=EADDGGGDGEG?GFFGDFFFFCAFGFGGFFGGDDF:DADGGDFGGGGFFFBFGBFDGFFDDDEBGFD	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:51:12803:14933/1	0	chrI	897	60	101M	*	0	0	AAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAA	GFFGFGGFGFGGDGGGGGGGGGGGGGGGGGGGGDEGGGGGGGGGGGGGGGGGGGGFGGGGEAACEEGGEEFAEEEGEEEDEEEEABDDGDDECDDCDDEDG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:51:2990:9988/1	16	chrI	897	60	101M	*	0	0	AAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAA	GGGEFGGFEGEFGFGFEFGGGGGGEGEGGGFGGGGGFGBGGGGGGGFGGGEGFGGGGGGGFGGGFFGGGGGGGGFGGGFGGFDGGEGGDFGGGEGGFGFGG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:50:10205:11996/1	16	chrI	897	60	101M	*	0	0	AAATTTGCAATTTTTATAGCTGAGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAA	GFGEEEEFEEEEEEEGEGAGGGEEFEEGEFEEBGEGGFFEEGGGEGGGGGDGEDGGFGGGGGGGGFGEGGGGFGGGGGGGGGGGGGGGGGFFEGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:99:12968:10523/1	16	chrI	898	60	101M	*	0	0	AATTTGCAATTTTTATAGCTGAGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAT	GFBGGEGGEGFGGFGGEEDEE?B>B5>5DCCFE:AFGG:GGGG?FGFDGGGEGGGGGGGGGGGGFFGGFGGEGGGGDDGGFGGGGGGGGGGGGGGGGGGFF	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:108:8721:8141/1	16	chrI	898	60	101M	*	0	0	AATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAA	D5:B=AFC?CF?FFFFE=FFFAFFDDDF=DFFFDFEFF=BFFFCFFFFFFFFFCFAFFFDFFFFFFABAFFADAEDFFFDFED?FFFFBBEDFEEDGFEGG	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:41:18519:16779/1	16	chrI	900	60	101M	*	0	0	TTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACA	BEEFFFE=BEEEBEEFEEAEEBD=>>EFBAF:FBFF:FFFFDDFDFFFFEEFFFFFFFEFFFEFEFFFFFEFFFAFFFFDFFFFFEDFFFEAEEEBEDEDE	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+BS-DSFCONTROL03:121:D0M8KACXX:5:1112:7827:74939	165	chrI	901	0	*	=	901	0	CATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTGAGACACTT	CCCFFFFFHHHDFIJJJJJJIIJJJJIJJJIIIJJJIJIIJJJJJCGIAGFHIIJJJJIHHHHHHFFFFFDCDDDDDDEDDDCDDDDECCD@3?@:<CCC	PG:Z:SNAP	RG:Z:000
+EAGLE:2:43:17839:20176/1	0	chrI	901	60	101M	*	0	0	TTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACAT	ADEFAFEFFFFFEDFEDFDDFEEFFFFEDFFFFFDFFFFDFBFDEAFBFDFDFFDF=FFFFFFFABEBEEFF?DFFADFFACCD5@B?B=C:@B@AE;?:E	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+BS-DSFCONTROL03:121:D0M8KACXX:5:1112:7827:74939	89	chrI	901	60	100M	=	901	0	TTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACA	DDDDDBDDEDEDEDDDDDDCCCCBBDEEDCFFFFHHHHJIJIJJJJIGHJJJJJJIJJJJJJJJJJJIJJJJIIJJJJIGIJJJJJJHHHHHFFFFF@CC	PG:Z:SNAP	RG:Z:000	NM:i:5
+EAGLE:2:119:2085:12525/1	16	chrI	901	60	101M	*	0	0	TTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACAT	?B?BBC@FFF=GGEFGACDD?ECACEFGEFGD=GEGEGGE?GGDGGGGGFGGGGGFGBGFGGFGDEFFDE?GEGGEFEGGGGGFGDGFGFGCCCEEDD<B=	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:1:37:2279:9702/1	0	chrI	902	60	101M	*	0	0	TGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATT	B><=??CCCAFDEFF=F?FFDF=D?AE?E@CFFABFEFFFEEE=C5@CA@FFFFFFFEE??BEDADA=DBEE?EBBEBEEDF>DBEEDEEEED:DEC@@5E	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:41:11602:1908/1	16	chrI	904	60	101M	*	0	0	CAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTT	EEEEEGDDEDBFEDBEDAE@CEEGEEEEGEFGGGGBGGFGGGGDGEGGGGGGGGFFGGFGGFGGGGGFFGGGGGGGGGGGGFGGGGGGFGGFFGGFGGFGG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:51:19603:11640/1	16	chrI	904	60	101M	*	0	0	CAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAAAAATTT	EEEEEED=DBBAEBBFE?E<?EEFGEEE?EEGFDGGGEGGGGEGDDGDDGGEGFGGGGFGGFGGGGGGGFGGEFGGGFGGGFGFFFGFGFFGGGGGGGGFG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:22:13011:15244/1	0	chrI	905	60	101M	*	0	0	AATTTTTATAGCTAAGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTT	FGGGDGGGEGGGGGFGGGGGFFGGFGEGGFGGGFGEGGGGDGGGGGGGEGGGGDEED=GGE=ECEGEFDBGFGEGE?DEDGEEFBEGEBDEEDEEEEEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:11:4741:6849/1	0	chrI	906	60	101M	*	0	0	ATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTC	GGFGEGGEEFFGDGFFEFDGGGGGGDCFDFGEGGGFGGGGEGGFGGFEGEGGGDGFFFGDEEBEEDEEEEGFFGGFFGGD5EECEFGAFF?FECCCFGGF=	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:17:12718:17138/1	0	chrI	906	60	92M1D8M1I	*	0	0	ATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAACTTTTTTCA	GGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGGGGDGFGGFGGGGGDFFGGGGFFGFFGGGGFGFFGFGGGFGGDGFGDFGGGGACA	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:84:4848:6681/1	0	chrI	906	60	101M	*	0	0	ATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTC	GGGGGFGEGGGEGG?GFGGFFFFFFCCFDCFFDEFDECEECFFFFGGFFBGFG?CEACED5C@AAEEBAEEABEEEBBDDEEEEEEEEDEFEEEE@BBBDE	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:70:5828:17291/1	0	chrI	906	60	101M	*	0	0	ATTTTTATAGCTAGGACACTTTTTGTCTGCCCATATATAGGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTC	FGGGDGGGGGGDGFGGBGD?FFFBFEGGGGGGFGFFFDFBFFEFFGGGGGGFGGAGGGG==EBCBDFFEEGFEEEGEBEFAEEBAEEGEGFGEGA@EDBED	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:88:11351:17404/1	16	chrI	906	60	101M	*	0	0	ATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTC	E?BBEBABBFEEEEEBGBCCEFEFF?AGGFEDEEFGGFGDGGGEGGGGFFGFFFEBGGGFFGGFBGGGGBGEGEFGGGGGGGGGGGBEEFEFEFFFDGGGF	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:21:9227:5397/1	0	chrI	907	60	101M	*	0	0	TTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCG	GEFEGFGGGGBGGDFGFFGGGGGGEGFFGGGGGGGGEDFGFF=BFBBDEEDFGCFBFEF?D@CEEECCEEB=EEEAEFFFC?BB:BFEFDC<6>8DDEDDF	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:96:7398:5258/1	0	chrI	907	60	101M	*	0	0	TTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCG	EGGGFGGFGGGGGFGGGGGGGGGGEGGGGGGGGDGFGGGGGGGGGDEGDFGG=GGGGGFEGDGGGGGEFGEGGGGAGDFFGFEECGEDEGGEEEEFGFDDG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:59:12995:3551/1	16	chrI	909	60	101M	*	0	0	TTTATAGCTAGGACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGG	GFGFEFDFEEEEDDCCCEEE?GDFEFDDBGEEGBGGGGGGGFGGGGFEGGEGGGGGGGGGGGGFGGGGGGGGGEGGFGGEGGGGGGGGFGFEGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:80:3409:18738/1	16	chrI	912	60	101M	*	0	0	ATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTT	EEEEFEDEBAFA@@DEEEEEGEFFGEGGFEGGGGGGEEDGGGGGFGEGGGEGFFGEGFGGGGGGGFGGFGGGGGBGGGGGGGGGGFEFFFEDFGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:77:1620:5914/1	16	chrI	913	60	101M	*	0	0	TAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTT	??@@??<A=?<??CCCEEEEEE@C=EEEEEAFEGGGEGGEEFGBGGFGGGGEGFGGGFGGGGGGGGGGGGGGDFGFGGGGGEGGGBFFFFFF:GGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:1:72:16563:11844/1	16	chrI	915	60	101M	*	0	0	GCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTT	AECCB=DDA=CED?DFEEAE:C??A?5EBEEBFFFF>FFEFE?EDE@>ACCAEFFFEEEEE=FFFFE:EEDDFEEFEEEEDDDDDB?:>>?ACC>C?DE?=	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:103:12336:20768/1	0	chrI	917	60	101M	*	0	0	TAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGA	FGGGGGGDFGGGGGGGDGFGGGGGGGGGGGGGGGFFGGGGGGCE=DGGDGGEGGDFA?EFGGBEDEGGGFEG?@GEE?FFBGGGDGFBGGBFF?A:A>;@E	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:33:12668:17941/1	0	chrI	918	60	101M	*	0	0	AGGACACTTTTTGTCTGCCCATATATAGGCAACCAAAAATTATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAA	FGGGGGGGGGGGGEGEGFEGDGFFDDBA?FFGDFGGGECDGFDFFFGE=G@EEDEE=BEE=EEECE?A:F=DEDD:E?EEC;@;DDEEADBE5@BEEB:5F	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:70:1514:14146/1	0	chrI	919	60	101M	*	0	0	AGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAA	DFAAFFDFFEEEC>E=DDDDDEEEDDEDEE?DEEEE?EEE@6CBBEABEB=AAAADBEEBEEDEDEE:EED@BE=AB?B8C?=EEEEEEEA<:@B?????:	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:41:3640:5327/1	16	chrI	919	60	101M	*	0	0	AGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAA	5FFF?CFFFFDEEDCE5=DE=EEEEDEEEEB?>@@DAEEDDBFAFFDDDD?DEFFDFFF>FEEDBEDEBFFDDDDD=C:A?FFDEFDEEDBEDEEDBBECE	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:70:16168:12341/1	0	chrI	920	60	101M	*	0	0	GACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAAT	GGGGGGGGGGGGFGGGGGGEGGGGGGEGBGGGGGGGEFGFGGGGGFGGEFGGGF=FFDBFFGCEGDEGFEEDBEGEBEE=@EGGEEBEFE==BBEDB:DAD	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:6:3780:20120/1	16	chrI	920	60	101M	*	0	0	GACACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAAT	C=B<??FEEBDG5DEGABCFGEB?FFFG?BGGGGFGFDFEFEEDD?FGGGGGGAGGFBGGGFGGFFFGFGGEGDGBGGFGGGFEGGGGGGGDGGGGGGGDE	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:38:5564:3174/1	16	chrI	921	60	80M1I20M	*	0	0	ACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAAAAATTTTTCGGGTTTTTTGAAAT	AEAA?DGEDDEGCEGDFGGGEGDGGDGGEFGGGGGGGGFGFGGGFG?FFFBGEGGDGEFFBGGGGGFFFEEGGGGGGGGFGGGGGGGGEGEGDEF?GGGGD	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:44:6557:3548/1	0	chrI	922	60	101M	*	0	0	CACTTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGA	GGGEGGGDGE=FFFEGEDFFCFEECGBDEGGGGG=GEEGGGDGEEE?EEEGGEGGEEFGGEFBEEC?A5CEDA=BEEE8EBEDADEEBAEBBBCC5@@ABD	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:23:12527:20440/1	0	chrI	922	60	101M	*	0	0	CACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGA	GFEGGGEGFGGGFGGEE:DDEE=EEGEGGDAFFA?GEGEGGGGDGGBGEGGG?GGEGGGGEGFGGFCGGDDBGBFF>BD@EGEGEFDFC=DDDEBD5?DBE	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:98:18353:7963/1	0	chrI	922	60	101M	*	0	0	CACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAACAATCATTTTTCGGGTTTTTTGAAATGA	@@C@>ACC:9>D:BDC:DD:DA?ABD?DDDD?BDDCC@>A?DDC5A<5A?BB?BBA=ACA@BBBBCC???@@??@A?AABBBBB@:=?8ABBBBB@@BB??	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:84:5126:1325/1	0	chrI	922	60	76M1D25M	*	0	0	CACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAACAACTTTTTTCAGGTTTTTTGAAATGAA	GGGGGGGGGGGGFGGGGGGGGGGGGFGGFGGGGGGGGGGGGGGGFGEEGGGGEEGFEGFGEGGFEEGEGG@BAEEEEEEEEEFEECD@9CCDDD=EEEE??	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:12:11478:10672/1	0	chrI	922	60	76M1D25M	*	0	0	CACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAACAACTTTTTTCAGGTTTTTTGAAATGAA	CAC?=:BBD;EFDFEFFDDFADFFEFFFFEFEFFDFCC=EFFD?EEBA?DDFCA;AEEB:DBFDEFEEBADBBDEEDF5=BEE?E?B=@B@BCC@B=ACB?	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:95:5316:12215/1	0	chrI	922	60	101M	*	0	0	CACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGA	GGGGGGGGGEGGGGGGGGGGGGGGGGGGGGGFBGDGGGFGDGGGGGEGGGGGEGGFEGGGEGGGEGFEGEEFBEEEC=;CEEGEEEDFA@CCCE=C=AEED	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:113:5213:14267/1	16	chrI	923	60	75M1D26M	*	0	0	ACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATATTTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAAT	=:;AABB??EB?B=DDA:EEEDDDEBB?EEEDEGGGEFEEGFEDC@@EFEFGGEEGCDB?ABA?5ADDGGGEF?AFGFGGFDFABFBDDGGF?GGGFGG5F	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:118:8721:7001/1	0	chrI	924	60	101M	*	0	0	CTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAAT	GGFDGGGCGGFGGFGGGDGGGGDGGGGFGBGGGGGADFFGGGGGEGFDFGEEGBACECBEBADFFEEBGEBBDBAC@CGGDBBDGB>EEEDFDDF@E:D@8	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:79:1079:9916/1	16	chrI	924	60	74M1D27M	*	0	0	CTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAACAATTTTTTTCAGGTTTTTTGAAATGAATA	;@EAEEEEGEEEEBEGGEGEGGAFEE@:@:;FEEFDEDEDGEGGFGBEBGGBGFGGGEDBGGEFGGGGGDGGGEFGEGGGGGGFGGGGDFFGGGFFGGGFG	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+EAGLE:2:85:1256:19207/1	16	chrI	924	60	74M1D27M	*	0	0	CTTTTTGTCTGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAACAACTTTTTTCGGGTTTTTTAAAATGAATA	ECEEC=FEFGDEGFEDCGFDFGFBGEGEEEGEFBGEEGDGFEEGGGGGBGGAGGGGGGBDGFGGGGGGGEFGGDGGGGGGGFGGGGGGGGGGFGGFGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:48:19056:2032/1	16	chrI	924	60	101M	*	0	0	CTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAAT	C;BAAAB:EDDEGEEEDAE@EDGGGBG:GDEGGEFEEFAGEAGGBGGFCEEGFDGGFFGFGGFGEFEECCAEDE@EGBFDGGDGGGGGEAEGGGFEGGGGF	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:1:76:11456:6131/1	16	chrI	924	60	74M1D27M	*	0	0	CTTTTTGAATGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATA	EEEEEEGGEEGDGGEEDGGEFFGGEEGEGEGGGEEGEGGGGGGEGEFGGGGFGGGFGGGGGG?GGGDGGGGFFDFFGGGGGGGGGEGGGGGGGGGGGFGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:1:61:3268:15848/1	16	chrI	924	60	74M1D27M	*	0	0	CTTTCTGAATGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATA	FEEDFAGDGEFEEEEAEGDEGGEGGGGGFGGEB=EACEEEBDGGGGEGGFFEEEE?DGBBFGGGDGGGGGGDDCD;DFFF?GGGGGDGGEGDGGGFDFFGG	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+EAGLE:1:86:4115:1680/1	16	chrI	925	60	101M	*	0	0	TTTATGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTA	?AA9BACFCCFGEEGFCFDFEEGDEGEGGGGDDGEEEDGGGFGGGFGDGGGGGGGFGGFGFGGGGFGGGGGGGGFGGFFGFGGGGFGGGFGGGGFGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:2:10237:11117/1	16	chrI	925	60	101M	*	0	0	TTTTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTTAAATGAATA	@:FFFFFDE?FFAFFFFFDDDDFEFFDADEEBFEFDBD?FFFFE?FFFDFFDDFBFEFFFFEAADEC<CCBEE:DEFFEBFFFEDDA?FFFBDDDDD=DCB	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:85:13088:19333/1	16	chrI	925	60	101M	*	0	0	TTTTTGTCTGCCCAAATATAAGCAACCAAAAATATTTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATA	:?GDBEEGBEEEDGEEEEEEEFEEEFGGGFFFEDFEGDGGGFGGBFGGGGGGGEFGGGGGBFFFEAGGEGGECEEBEAGGFDGGGGGEGEGGGGFGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:1:79:18345:10394/1	16	chrI	926	60	101M	*	0	0	TTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTAT	EBBBCBEEBBBEGBGEFEFDGBEBD>DGGGGBEFAGEFFE?GGEFGEGGEGFGFGGFFFFBFGDGGGGGGGE=EDEEEGAGGGFGGDFBBFGGDGGGGGFF	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:11:6740:20191/1	16	chrI	927	60	101M	*	0	0	TTTGTCTGCCCAAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATC	AA?@?BAAB=A?::::??B?AB>E>??@EAA@:@?<@>>==@@:CA=D?DBDBB@EEGGEBGEEEE?AEBAADDBBGDBGGGEFGGBGEGGEEEEE5DABD	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:1:39:17435:10955/1	16	chrI	927	60	101M	*	0	0	TTTGTCTGCCCAAATATAAGCAACCAAAAATATTTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGATTTTTTGAAATGAATATC	E=D@DFEE=:?GDEBFEEEE?E=ECCBEGDBGGDFBGGGGEFGDGFEEBEAFGEGGGGGGGDGFGAGGGFFEGGGDFGGDGEEEE:GFBGGGDEGFGEGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:83:18991:16122/1	16	chrI	927	60	74M1I26M	*	0	0	TTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAAAAATTTTTCGGGTTTTTTGAAATGAATAT	EABA5?DEFFEEC5@BDDFEFDFECCFFFFFACA:?=EFFFEBDEAFBFBBEE=DEAAF=FBDDA>F>BFFEEEED?DD5?GGGGF=EBGG?BGFFFEFFB	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:23:6768:7044/1	16	chrI	929	60	101M	*	0	0	TGCCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTATCAT	FBB??E?CB@??C=B=C?=?:E=CCD@AA?:5E5EDF=FFFDFFFBBEBEEEC:EEEBCBAFFEFDDFDDFADEEFFBFDFFEFFDFBBEFGDGGDAFFDF	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:1:47:19241:12210/1	16	chrI	929	60	101M	*	0	0	TGAATGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCAT	GGGEGGEEGFEEGGGFGGGEEFGGEGGGGGFGGGGGFGGFGGGGGGGGGGDFGFGGGGGGGFGGGGGGFEFGGGGGGGGGGFGDEEGGGGGGGGGGGFGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:1:79:9772:3464/1	16	chrI	931	60	101M	*	0	0	TCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAG	=ECCBB=?FGFG?GGGGGBGGFEGDCBFFF=EFBFGGFGGGDFGFFGGGGGEFGGGGGBDGCCCEEFEFFEFFEFDGGGGEEGGDGGGGEGDFGGE?GFGG	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:29:4194:2200/1	16	chrI	932	60	101M	*	0	0	CTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGC	@:AE5DDEEBE>@@=5BBB8G@DFDFGGEEBEE=BB=GGGEEEEDEFEFFFADGBGEGGGGFBFGAGFGGGGGGDGDEDFGGGGGGGGGGGGGGEFG?GGF	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:1:32:7810:14929/1	0	chrI	933	60	101M	*	0	0	TGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCT	GGGGGGGGGGGGGFFGEGGGGGGGGGDFGGGGGGGFGGFGDFFDEGEGGEGGGGGGGFGGGBEGGGECGFGGGFGGGB5GFFFFA:CEE:B5?B?FDEAFE	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:38:7229:15314/1	16	chrI	933	60	1I65M1D35M	*	0	0	ATGCCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCT	G?EFEDFE=FFFDGFGGBFGGCFDBAGG=FEEGDGFGGGGGFGGGG?G?GBEFEAEGGGGGEEEAAGGGDGGGGGGEGGEDGGGGGFGGFGGGGEGGGEGE	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:45:5717:2359/1	0	chrI	935	60	101M	*	0	0	CCCAAACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTAC	GGGGGGGGFDFFFGDGGGGGGGGGGGGGGGGGGFGGGGBEGGGGGGGFGGEGGFEDGEDEFGGEEFFGEFGGBBGEEEEBEE:CD:AAAADEEEEE?CCC?	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:67:2263:13075/1	0	chrI	935	60	101M	*	0	0	CCCAAATATAAGCAACCAAAAATATTTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTAC	EDBEEEEEE?E?EEDDEEEEDDEECEDEEA>6C<@>BB>9?ACAAEECEE?CCEE?C:=B5AB??AEEEEB@DDD:>>@@C:?@??@:?@BCAC@@?A@?<	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:94:5592:18698/1	16	chrI	935	60	101M	*	0	0	CCCATATATCGGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTAC	ECECBEABA9AAEE@C;EDGGFEFGGGDFGFGEEEFFFFGBGGGGGFEAGCDGGFGGGGGGFGEFGGGGGEGGGEDGFGGGGGGGGGGDGFGFGDFGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:59:10637:1223/1	16	chrI	936	60	101M	*	0	0	CCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACA	CAEBDCA?EECEDDF@@GGGEGEEEEDEGEEFGGGFGEGFEEGGGGEDDE:FFFDACECDFEGFCGGFGFGGGFGAGGGFGGGBGGFGGDGGGGGGFGFGG	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:1:70:4295:15837/1	16	chrI	937	60	101M	*	0	0	CAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAG	FCCBCB=CECBEEFEEGEGGEGEEEEGGGGEGEBEEEGGFDGGGGGFDDGBGGFFGGGGGFBGGGGGGGGGGGGDFGGDFFGGGGGGGG?FEEEEDBEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:64:5817:4431/1	0	chrI	938	60	101M	*	0	0	AAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA	GGEGBGDEEGGGGGDGGGDGGGGDGFFDFFGFGGGGGEGGEFEFDGGGGGDAFG?BEEBBFGFGGGGGGGGECGFGEFAEGGGF?FEF:A@?@C:?:@@@E	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:8:6755:19986/1	16	chrI	938	60	101M	*	0	0	AAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGA	BBEA==FEBAFFEFFFDADEE=D=CEE=D=;FFFDDFFFDEEEFFA=FB=FEFFFFEFAFFDDDFEFFDFDFFFF?DDFFFEDFFDFDFEFCDDECD:DAD	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:105:14800:10824/1	16	chrI	938	60	101M	*	0	0	AAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTGGCTACAGA	:GF=EEGGECDEFFGGFGGGGFFBEDDEF@EDGEEGCECEEE5EEEEFEF=FFGGDCGGEEGGDGGEGGGGGGGGGGDFGFGGFGFEB?EEE:EEDFFFFF	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:71:6398:5629/1	16	chrI	938	60	101M	*	0	0	AAATTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA	BCCAC:DFD?FF?>ECDDF?DEF?=FF?F?FFF?BFDFD?FDEEA=B??EE?E:B>@ACC5EEEEEDDBDFBDEBDEEDEDEEEE?ED:BECABECBBDA:	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:84:15268:14389/1	16	chrI	938	60	101M	*	0	0	AAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA	D:A@A?5:BCA?FCCCABDEEA@@5BFBAADEEDFFEBBBCEBEEED=5DEDDCCAFFFEDDADBD=5FFFDED=AEFDFFFDFFFF=FDDFAFFFEE:EE	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+BS-DSFCONTROL03:121:D0M8KACXX:5:1303:1422:86454	99	chrI	939	60	100M	=	952	113	AATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA	CCCFFFFFHHFHHJJJJJJJJJJJIJJJJIGJJJJIJJIJJJJJJJJJJJJJJJJJIIJJJJJHHHHHFFFBDCD@BDDDDDDDDEEECBDDDBDEDDDD	PG:Z:SNAP	RG:Z:000	NM:i:3
+BS-DSFCONTROL03:121:D0M8KACXX:5:1308:20909:7788	73	chrI	939	60	100M	=	939	0	AATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGA	@@@DFFFFFHHHFFHIIFGIHIIGJJJIGFGHGIGDGG9DGIJBHIJJIIHGHGHCG?A@BGDAECHFFDB;?CDDDD@CCDDC:CAD:?CB<<CD>AAA	PG:Z:SNAP	RG:Z:000	NM:i:3
+BS-DSFCONTROL03:121:D0M8KACXX:5:1308:20909:7788	133	chrI	939	0	*	=	939	0	TGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATT	CDDDDC>D@@<>@ADCDEDEECCDBFFFHHHIIHHGBIIIGEHAIJJJHFJJJJJIHFFAJJIHCEIJIIGJJIIIIIHGHEBIIJJHFHHHFFFFF@@C	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2304:7438:34489	99	chrI	939	60	100M	=	951	111	AATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGA	CCCFFFFFHHGHHJJIJJJJIJIGJIJJJHIJJJIJJIJJJJJJJJJJIIJIIJJJJIJJJIJGHHGHFDFADDDBDDDDDDDDDCDDEDCDDDDEACDD	PG:Z:SNAP	RG:Z:000	NM:i:4
+EAGLE:1:25:14090:18624/1	0	chrI	939	60	101M	*	0	0	TATATAGGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAA	FCFFFGGEGGFGGGGGGGGGGGGGGGGFGEGGGDBEGGGGGGDEGGGGGGGGGGGGEFFEEEEFE?EEDEEC:EBECEEEEFGEDDFE?FBEE@DDDDD=F	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:1:33:5876:3688/1	0	chrI	940	60	101M	*	0	0	ACATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAA	GDBGEFFGGDGGDGGGDFBG@GGFGGFEGFFGGEDGGFDEGGFEFBFFFBC?E=CB?ECAGGG=DGGFFFEFGFGGG??GE::55BCAEDED?EC5D5CC9	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:55:14770:18631/1	16	chrI	940	60	101M	*	0	0	ATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAA	FEGBEEEGEEFG@GFGGFGGFGEGGEFGGEFGBGEGGDGGGGFFGGEGGDGGGGGGGEEGGGEGEGGGGGGGFGGFGGGEGGGGBGGGGGGGFDGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:93:9444:3777/1	16	chrI	940	60	101M	*	0	0	ACATAGGCAACCAAAAATATTTTCCAAGTTTTTTTTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAA	FFGADEGFFEGGEGGBGGFGGGGBGEEGGGGGGGGGFGGGGGGBGGGGDBFFDGGGGAEGGFGGGFGGGGGEGGGFGFGGGFFF=FDGGDGGGAGGGGFGF	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:50:5922:5719/1	16	chrI	940	60	101M	*	0	0	ATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAACATCGTAGCTACAGAAA	:???:???5:==8@EECCC5CA?5ECACCCEFEEEEEEFGGGGDEEEGEEEGEEEEEEEGGFGGEEFGGGGEGEGEGGGGFBAAD:GGGGGGGGFFGGFGG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:21:17413:6903/1	0	chrI	941	60	101M	*	0	0	TTTAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAAC	EEBAD:E?DAFFF:FDDEBEGGGDGDGFEFGFGFGGFDDGEEGGGFFGGGGGDG?CDGGGFDFFGEEEEACEC=EEFGGFBG?G?FDGF=?A=D=FFF5F5	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:1:120:7503:14055/1	16	chrI	941	60	101M	*	0	0	CATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAAT	EEDEB5GFEEGFGGGG:=GGFDDFFFAA7EEG:BBGGDFEGGADG=GGGBGGGGGGGEGGFGGGFGGFFFEGEGDGGGGFGEGGEGGFGGGGGFGGGGGGF	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:91:17352:13444/1	16	chrI	942	60	56M1D45M	*	0	0	ATAGGCAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACATAAATGG	@CCBCDE?EEEEEEAEEEEEEGBEE>EEDEEGDGEBFEEEGGE?EGFFGEGFFFECFBEFGDFGGGGGGGGGGGGGFGGGGGDFGGFGGGGGGGGEGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:97:3188:16851/1	16	chrI	943	60	101M	*	0	0	TAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGG	:B??BB5=8CCEEEEADABDECB:EA@=?BB5C>C;:DAA::A=?D:ECCECEEEEC??C???=D?C??=?DB==?D?BDD:E=AEEEEAEDC?C?DDDDA	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:85:10558:18446/1	0	chrI	946	60	101M	*	0	0	GCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAG	FGGGGGGFGGGGGGGGGGGGGGEGGGGGGFGFFGGGEFEGFGGEGGDGEFGGGEGDGGGCGGGFADEFFFFEBFEFBEFFEEGEGDBEEEDGEEEDDE?=A	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:35:15009:8527/1	0	chrI	946	60	101M	*	0	0	GCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAG	GGGBGGGGABGG@EGGGGGGGGEGFGGGEGEFGGCGEGGGFEGGEGEFD?FGC=CEEGGEEDFE?DDFEFAACE=ECCCC?GDEEEEBBCEE@EECCB?CB	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:62:14360:14144/1	0	chrI	946	60	101M	*	0	0	GCAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAG	GGGGGGGEGGFGGGDGGGGGEGFGGGFGGFFFGDGFEEFFGGGFEFGEGGGGEGEDGGDEFFFFADEFDEEEEED?EEEEEEGEEDEEEEEEEEEABB7AA	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:1:81:13994:10137/1	0	chrI	947	60	51M1D49M1I	*	0	0	CAACCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCAGGTTTTTTGAAATGAATATCATAGCTACAGAAATGGTTGTA	EEEEEB?EEDBFFFFFFFFFFBFFFFFCCCABCC5?DDD?FEEDFEFEFDBFEEFFFFFDFFEEEEFFEEBEEEEDDEBEEEEEEEEEEEEEEEE@?@D?@	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+EAGLE:2:69:16589:8289/1	16	chrI	947	60	101M	*	0	0	CAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGT	@A???;>BA@DB:DA=BBB@@AEEDEADEEEEFBEFDFDDEBFEEEAAEBB?FEDFFFEFFFDFFDE:FFFEF?FFFDBFFF?EFDEEEEEE=?DEFDFFF	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:1:46:11875:10933/1	16	chrI	947	60	101M	*	0	0	CAACCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGT	=EBDDGEGEGDFGBEEGBEGGGGDFDGAGGGDGGGEEGBGGGGGGGCE?EEAD=D?GGGFGGGFFDGGGGGGFFGGFGEFGGDGGGDFGGFFGDGFGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:70:7204:17965/1	0	chrI	950	60	101M	*	0	0	CCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACA	GGGGGGGGGGGEGGGFBFDFGGGFFDFAGGGBEGGGEFDFEGEEGEGEEE@EFFFFGGGEEGGEEDBDEEEEEEDEEGGEEAB=AEEE@EDD?D@?B9@B=	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:47:16300:14806/1	0	chrI	950	60	48M1D53M	*	0	0	CCAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAATGGTTGTACAC	GGGGGGGGDGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGFGGGGGGGGFGGFGGFGGGEEGGFGGGGGFFDFEGGEGFGDGEGGGGGGGEBFAECC?EEA	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:72:3307:18932/1	0	chrI	950	60	51M1I49M	*	0	0	CCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAAAAATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTAC	EEDEDDFFEEBGGGFGGFGGGDFGGFGEGGGEDGGEEGEGDFFGGEEGGEGEEFGGGAGFFADFFCEB?CEEDEDDCBCEBDBCCCECEACCACE;??;::	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:55:6957:9011/1	16	chrI	950	60	101M	*	0	0	CCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGAAACGGTAGTACA	:A??BB?AAB=?=BBBBCCDC?BDDDBCDBBCDB?DB?:DDADDDDDD@?=DADD?DDDADDDDDDCDD:DDBD?:DD?DD?DDDDADDD?ACA>ADDD?:	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:41:5354:20962/1	0	chrI	951	60	101M	*	0	0	CAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACAC	GGGGGGGGGGGGGGGFGEGGGGGGGGGGGGGEGGGGGGGGGGGFGGGGFGGGGGGGFGGBEEGEFB=EEEEBED=BBCABEBEBEEEEEEAAC<=A<:@AA	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2304:7438:34489	147	chrI	951	60	1S99M	=	939	-111	CCAAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTAC	#DDEEEDCADC@CDDDDDDDDDCDDDDDDDDDDEDEDEEEDEDFJJIHD@IIJJJIJJJJJIIGJJIJJIFGJGIIIJJJJJJIHGJHHHHHFFFFFCB@	PG:Z:SNAP	RG:Z:000	NM:i:4
+EAGLE:2:48:13688:1594/1	16	chrI	951	60	101M	*	0	0	CAAAAATAATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTGCAGAAATGGTTGTACAC	@EEEEE?BEEEADBAC:EEEEEEEEEECEBEECEEEBEEDEEEEEE>>A@=BFDEFGGEEFGEEFEFGGFEFFGGFGGGGGDGGFGEFFFFAFFBFGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+BS-DSFCONTROL03:121:D0M8KACXX:5:1303:14609:5903	165	chrI	952	0	*	=	952	0	CTTCTGAAAATACAAAAAAGTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTT	CCCFFFFFHHHHHJJJJJJJHHJIJJJJJJJJJJJJJJJJJJJIJIJJJJJJJJJJJHHHHHFFFFFFEEEDDDDDDDDEEEDEEDDACDDDDBDDEEEA	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2106:7309:94230	165	chrI	952	0	*	=	952	0	CTTCTTAAAATACAAAAAATTTGCAATTTTTTTAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAATAATTTCCAAGTTTTTGATGATTT	CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHHFFFFFEEDDDDDDDDEEEDEEDDCDEDDDDDDEEEE	PG:Z:SNAP	RG:Z:000
+EAGLE:2:94:15425:16067/1	0	chrI	952	60	101M	*	0	0	AAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACT	FEGFGGGGFFFGGDGGGFGGFGFFFEGDFFEGGGDEEDDGGFDFGGEEGGDEGFGGGGBDEFFFFEBEEGBEEEEEGEEEEEEFEGDEEEBE;EE?BCCCC	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+BS-DSFCONTROL03:121:D0M8KACXX:5:1303:14609:5903	89	chrI	952	60	100M	=	952	0	AAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACAC	DEEEEDDDDDDDDDDDDDCDDDDCCDDDDEEEEDEEFFFFFHHJJJIJJJJGJJJIJJJJJJIGHJJIHGJJJIJJJJJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:5
+BS-DSFCONTROL03:121:D0M8KACXX:5:1303:1422:86454	147	chrI	952	60	100M	=	939	-113	AAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACAC	CEEEEDDDCCCDCCCBDCADDDDDCDDDDCDDCEDDDDCCDFHIHHCHJJJJJJJJJJJJJJJIIJJIGIJJJIHIJJJIJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2106:7309:94230	89	chrI	952	60	100M	=	952	0	AAAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACAC	CEEEEDDDDCCDDDDDDDCCCDDDDDDDDDDEDEDEEDEEDFHJJIEIJJJJJIJJJJJJJJJIIJJJJIJJJJJJJIJJJJHJJJJHHHHHFFFFFCBC	PG:Z:SNAP	RG:Z:000	NM:i:4
+EAGLE:1:48:14890:12005/1	16	chrI	953	60	101M	*	0	0	AAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTC	CEDF@C@EE=EEDEGEEBGEEEEDEBEFB?BEAEE?FDCC>CFFDDG=GEGFCGGGGGFEGDBGGGGAFGBFGAGEGEGFDFGGGGGGGGFFEGGFGGGGE	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:1:67:18282:4840/1	16	chrI	953	60	4M1I15M1D42M1D38M1I	*	0	0	AAAAATAATTTCCAAGTTTTAATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCT	>A9EBCDAD@@EFDEFBDFGFGEGGFEEE?EGFFGGEECCC@?:BAFBFDFGGGFGGGGGFADFGGDGDGGF?FFFDFFDFEEEDDBEEEBFFBFFGEGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:50:13587:7527/1	16	chrI	953	60	101M	*	0	0	AAAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTC	GEFEEEEGEEFEEBDGEDGDFGGGEEGFGGGGBEGGFGGGGGGGGGGGEGFGBFGGGGEGGGBGGFFGFGGFGGFGFGFGGGFGGGFDGGGFFFFFEEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2204:13467:2490	165	chrI	954	0	*	=	954	0	TTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAGTATCATAGCTACAGAAACGGTAGTACACTCTTCTGAAAATGCAA	CCCFFFFFHHHHHJJJJJIJJJJJJJJJJJIJGIJJJJJIJJBFIIJJJIHHHHHH@DFFFFFFEEEEDEDDDDDD@BDCDDDDDDDDCDCDCC@>::>C	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2210:13891:35020	165	chrI	954	0	*	=	954	0	TGGAAATTATTTTTGGTTGCTTATATTTGGGCAGACAAAAAGTGTCCTAGCTAAAAAAATTGCAACTTTTTTGTATTTTCAGAAGAAGAGTGTACTACCG	EEDEDDDDBDBDDDDDCCDDEEEDCBCCCEEFFEDFHHHGHIIJIJJJIIGJJJJJJJIGGGGGFIIEJIGDGJIIIIHEFJJJJIIHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000
+EAGLE:2:71:10251:4806/1	0	chrI	954	60	101M	*	0	0	AAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCT	GGGGGGFGGGGGFGEGGGGGGGGGGGGGGBGGFGGEEFDECEGGGEEEGGFGGGDGCCFEFGEDDEEGEEEEEEEEEDEE@GEEECDCDD?CC>CCAACCE	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+BS-DSFCONTROL03:121:D0M8KACXX:5:2204:13467:2490	89	chrI	954	60	100M	=	954	0	AAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTATCATAGCTACAGAAACGGTAGTACAATC	::>C@CA>:CCCDDDDDDDDDDDDDDEEEDCFFFFFFFHJJJIHCJJJJHJJJJJJJJJJIIIJJJJJJJJJIJIIJJJGHJIIJIHHHHHFB=24=+B=	PG:Z:SNAP	RG:Z:000	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:2210:13891:35020	89	chrI	954	60	100M	=	954	0	AAATAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTC	CCDC@A@@DDDDCDDDCDDCDCDDDDDDDEEEEDEEFFFHHHJJJJIJIGJHGJIIJJJIJIIJJJJJJJIJJJJJIIJIJIJJJJJHHGGHFFFFDCC@	PG:Z:SNAP	RG:Z:000	NM:i:5
+EAGLE:2:50:6355:12073/1	16	chrI	957	60	101M	*	0	0	TAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCT	?C=@CA=EDCDBEGEEGEEAEDEBEFDFBCEGEEGGGGGGEEFFEEGGFGGGGGGGGGGGGGGDGGGGFDGGGGGGGDGFGGGGGFEEEEEFGFFGGEGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:65:16283:15152/1	16	chrI	957	60	101M	*	0	0	TAATTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCT	EEEGEEEEBBDGGEGGECEE:BFGGGFFGEGFGEGDC?EEDCEEEAGEAGGGFGGGGGFGGGGGGGGADGGGFGGGGFFGGGGGGGGGGGEGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:79:12640:7363/1	0	chrI	958	60	101M	*	0	0	ATTTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAACGGTTGTACACTTTTCTG	FFFEADDDDD?=DD;A:::C=ADDBB?DCDEDFFD?EDEEAAEEAFDFDD=C?:CD?DBAEBEEEF=FAAEEDEE=CB=BBEBBBDBDD?ED@B@D@ED@:	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+EAGLE:2:65:16789:19099/1	0	chrI	959	60	101M	*	0	0	ATTTCCAAGTTTTTATTGATTTGTTGCATATTGAAAAAAACTTTTTTCGGGTTTTTTGAAATGAATATCATAGCTACAGAAATGGTTGTACACTTTTCTGA	EEDDDCB?DBACCCCCC>EEEEEAEBBDDBEAEBCDEEDACEEEEEEEEEE@EEEEEBBEE?EAEEEEEBEEEEEEEEEBDEDEE@BDBB@B@@BBD@<DB	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+EAGLE:2:42:12059:15505/1	0	chrI	960	60	101M	*	0	0	TTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAA	GGGGGFBFFFFBDFEGGF?GGDGEDFGEDFFFFDFGFGGDACEFEFFFFFAFEFFFEFGGEFG:DB=ECBBEEBBEEEDFEEEA>>=8>=A@@@AE@==BA	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:56:5751:3283/1	16	chrI	960	60	101M	*	0	0	TTTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCT	AA@ABA5AFFFFBEEGDDE=FEF?AF?EEDA>>@>EEFAFCCCCC=DEG?FGEBFAGGFBGDDC5C5DFFD=GDGGEEEDGGGGBGGGGFFGGGDFGGAEA	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+EAGLE:2:7:4422:19468/1	16	chrI	961	60	101M	*	0	0	TTCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAA	GBEEE?DBFFFGG:GGEGFCGGDECBFBFFD@@>D5ECEEAGEFGGGGGGAFG:GGEEEEEAFFFDGGGGGEE=EDGGGGGGGFGGFFFBFGGEGGCCGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:24:3469:6979/1	16	chrI	962	60	101M	*	0	0	TCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAGTATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAA	EBB=BDGFFGFEFAF:GGGEGFAFEBDDEGGGGGFG=DDDGGGGGGGEFGCFFFGFGBGGGGAAFGGDGFGEGFGGFGGGGDFGFFFBFFAGGGGGGFGGD	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+EAGLE:2:107:10805:16122/1	16	chrI	962	60	101M	*	0	0	TCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAA	DA?C=EFABFGEC=DBEFFCFCCBBCE=ECCCCEFE:FAFGGGEEEEBDDEGG:EEFGEFG?AFFFFFFEF=FGGGGGGEGGGGGGFAGGGDGGGGGGBGG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:103:2608:5570/1	16	chrI	962	60	101M	*	0	0	TCCAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAA	GEFDEBGFGGGDEFFCDEDEAGGFBG=GGGGCCFDEDFCBFGGGGGGGDGGGGGGGGGGGGGEGDGGGGGGGFGGGGGGFGGGGGDGGGGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:103:8177:14669/1	16	chrI	963	60	101M	*	0	0	CCAAGTTTTTGATGATTTGTTGCATATTGAAAAAATCATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAAT	EGBFCEEFAFEEEEEGGFEFGEEEEEGFEGGGGEEGGEEGGGGGEFEEFGGFGGGGGGGGGGEGGEGFGGGEFFFFGEGGGGGGGFGGGDGFDGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:77:9271:19697/1	0	chrI	964	60	101M	*	0	0	CAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATA	GDGFDFGGGEFGFGGGGFGGGGGGGGGGGGGGGGGGFGGGFDGGFFCFGFGGGFGGFFGGGGEGGEGGGEDGFEEEEFFDAEFCDGEGBEEGEEFEEEGEG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:88:12652:14437/1	0	chrI	964	60	101M	*	0	0	CAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATA	EEDEBEDEDEF=FFBFCFEAFFBFFFBFDF?DFFCE@=FDBBFEEC?BEAFEBEBFEEEEDEEFEBBEF@BE?ABAEDDB5==6=<@?@@@@=@B@A:AAE	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:66:2841:12985/1	16	chrI	964	60	101M	*	0	0	CAAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATA	EBEEGGG=DFEGGEFDGGGGGFEGGFDFGGCFFE?FFGAGGGGGGGGGGGGFGGGGGFGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:47:10826:17209/1	16	chrI	965	60	101M	*	0	0	AAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTGAAAATAC	EDDDDBEEGEEEEGEFEGEG?EEFFFEEEGGFECGDGBEGEGGGGGFGGGFGGGFGGGGFEGEFFGGGGEEGGFGGDGGFGFFEFE5GGGGGFGGAEEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:42:13795:17461/1	16	chrI	965	60	93M3I5M	*	0	0	AAGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAA	C?CFDFBGEEFE<GGGDDECDAGBFE@CCCFE=EGEFEGGBGGFFGGGGEGGGGGGGGFGFFDGDGGFGBGGGGGEGGGGGGFGGFGGGGDGGGGGGFGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+EAGLE:1:64:12381:18587/1	16	chrI	966	60	92M3I6M	*	0	0	AGTTTTTGATGATTTGTTGCATATTGAAAAAAACATTTTTCGGGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCTTCTTCTGAAAAT	EBBEEEGGDGGAEFEGGGGEEEGGFECCCFEC=EBE=GGGGGGGGEGGGFGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGFGFGGGFGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:1316:21338:23882	165	chrI	1001	0	*	=	1001	0	TAGCTACAGAAACAATAGAACACTCTTCTCAAAATACAAAAAATTTGCAATTTTTATAGCTAGGACACTTTTTGTCTGCCCAAATATAAGCAACCAAAAA	>==AA>>AB4=AABCBAB+<3<ABCCB7?+A@A<ABABBBAAABBB>BB7AAABAAB78)9)3=BAABABBAAAA9;?9;;.;>@?B=;=??>:=199=9	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:1316:21338:23882	89	chrI	1001	60	46S53M1S	=	1001	0	NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTCGNGTTTTTTGAAATGAATATCGTAGCTACAGAAACGGTAGTACACTCN	##############################################@;C<-0(#??)<????HGD<CFFCE?CACE9B<HGICHEHGFFDHFDDBB:11#	PG:Z:SNAP	RG:Z:000	NM:i:3
+EAGLE:2:100:13144:11902/1	16	chrI	1359	60	101M	*	0	0	TTACGTAATAGCTTCTTTGACATCAATAAGTATTTGCCTATATGACTTTAGACCTAAAATTGACTATTAATGCCAATTTCATGATATCTAGCCACTTTAGT	EAAB?@=AB:BBDDDDA@?==@ABE=B:EBB?DE=BFBDBDFDEBFEBGEEDGFAFFAFFBDDFE=GFGGGBE=EAFE?GGDGDFFEFBBGGGDFGGGGFG	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:48:18830:9303/1	16	chrI	2070	60	101M	*	0	0	TTTGTGGGGAAATATATGTTTCTGACTCTAATTTTGCCCCTGATGCCGAATATCGATGTGAAAAAATTCAAAAAAATTTCCCTGATTTTATATTAATTTTT	CCDD=FEEBEEEEEEEEEFEEEEFBDEEEGEEEEEGGBDGFGEDGGGGCGGEFGGGGGDGGGGAEGFFFGGGGGFGBGGFGGFGGGGFGFGDGFGFGGGFG	PG:Z:SNAP	RG:Z:266-1	NM:i:2
+EAGLE:2:28:7318:20945/1	16	chrI	2075	60	101M	*	0	0	GGGGAAATATATGTTTCTGACTCTAATTTTGCCCCTGATACCGAATATCGATGTGAAAAAATTTAAAAAAATTTCCCTGATTTTATATTAATTTTTAAAAT	CEEEEEDDBDCADB?DGEEEEFBGGDGEBGGDGFGFGGGFEGFGGGGGGGGGFFGGGGGGGGGGGGFGGGFGGFDGGGGGGGGGGGFDGGGGGGGGGFGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:74:12831:9830/1	16	chrI	2094	60	101M	*	0	0	ACTCTAATTTTGCCCCTGATACCGAATATCGATGTGAAAAAATTTAAAAAAATTTCCCTGATTTTATATTAATTTTTAAAATACGAAAATCCATTGGATGC	=CC=E=GGGEGFFBEDDFFFAFFF?FDFGFGGGGGGEFDGDEDGGGGGGGGFGGGDFGFGGEFDGEGFGGG?GF?GCCCCCD8DDDGFEEGEEEFGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+EAGLE:2:26:9616:12584/1	16	chrI	2512	60	101M	*	0	0	TATCATTAATAATATTTATCAATTTTCTTCTGAGAGTCTCATTGAGACTCTTATTTACGCCAAGAAATAAATTTAACATTAAAATTGTTCATTTTTGAAAA	=FBEEEEGEEEE?EDEDFEDEEA?EEFGEGFEGEDEDFGFEFDGGGEGDEEGGFGEDGGGFGFGGGGGGFGGGGGGGGGGGFGFGGGDDGGAGGEEGGGGF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1305:10647:87203	73	chrI	2547	60	71M1I28M	=	2547	0	GTCTCATTGAGACTCTTATTTACGCCAAGAAATAAATTTAACATTAAAATTGTTCATTTTTGAAAAAAAAAATAATAAAAAAAACACATTTTTTGGAAAA	CCCFFFFFHHHHHJJJJJJJJIJGIJJJJJJJJIJJJJJJJJJJJJJJJIJJIJJJJJJIJJJJJJJJFDDD?CD;ACDDDDDD<B8<>CDECDBBDDDD	PG:Z:SNAP	RG:Z:000	NM:i:2
+BS-DSFCONTROL03:121:D0M8KACXX:5:1305:10647:87203	133	chrI	2547	0	*	=	2547	0	AAAAACACATTTTTTGGAAAAAAAATTGTCCTCGAGGATCCTCCGGAGCGCGTCGAATCAATGTTTCCGGAACTCTGAAAATTAAATGTTTGTATTATTG	@DCC@C@DDBDDCDEDDDDDDDDDDBDDDDDDDDDDDDDDDFFHHHIJIIJJJJIJJJJJJJJJIIJJIIIFJIJJJJJJJJIJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000
+EAGLE:1:84:3615:2746/1	0	chrI	2728	60	101M	*	0	0	TTAAATTATTGTAGAACCCTTTCGTTATTGAGATTTGATAACTTTTAAGTAATAAAATTTTCGCAGTAAGACATTAAAACATTTCACAATTAAGCTGGTTC	GGGGGFGGEGGGGGEGGGGGGGFGFGEGGFGFGGGGGBGGGGGGGGFGGEEFAGDGEEGGGFEGGGEGGEGFGGEEGFEEEEEEGEGGEEEBFDD?@68?;	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2110:10822:7981	73	chrI	2900	60	100M	=	2900	0	TCCCAATATAAACGATGTCAGTTCTGCGACATGCTACAGTCATCCACTAAAGTAACCCGAATACCGACAAAAGAAGAGGAACGCCAACTTTGGATAGACG	CCCFFFFFHHGHHJIJJGIIJIJJJJJJJJJJJJJJJGIIIJJJJFHJJJGIIHIJJJJJJJJGHHHFCAEDDDDCCDDDDBDDDDDDDDCCDCCDDDDD	PG:Z:SNAP	RG:Z:000	NM:i:2
+BS-DSFCONTROL03:121:D0M8KACXX:5:2110:10822:7981	133	chrI	2900	0	*	=	2900	0	GGCAAATATTATTTAAAATCAATCAAAATATAAAAAATAGTTCGTTCGCTGAAAATTTAAGCGTTTTCCGACCAAAATCAGCCCCTAGAGCGTCTATCCA	CCCFFFFFHHHHHJJJJJJJJJJGIEHIJJIJJJIJJIJIHJIJIIJJJJJJHIHIJJJIJJIHIHHHHFECDBD?BDDDDDDDDDBDDDDB<@BDEDCC	PG:Z:SNAP	RG:Z:000
+EAGLE:2:60:6530:16775/1	0	chrI	2909	60	101M	*	0	0	AAACGATGTCAATTCTGCGACATGCTACAGTCATCCACGAAAGTAACCCGAATACCGACAAAAGAAGAGGAACGCCAACTTTGGATAGACGCTCTAGGGGC	GGGGFEGGGGGFGGGFFGGFGGGGGGGGEGEGGGGFGGGEGFFEDGGFGGGGFGGGCGGGEEFGEEGGGDBEGEGGEEEEEEBG?EGGBEFEEDDEEEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1204:4496:9204	99	chrI	3991	60	100M	=	4394	476	GTGCGCCGCGACTCCGCCCACCGGTGGTAACTTTTTGTGGGGGCATCTTTGTTTTTGGTCATTTTTCAGCGCTTTTCAGCGATTATTGACCAATTTTGAA	?=??;=@DDA1:?DBD:D::?@0;-;;-==7=C>>C8?A;>@8'3<5>AAAA????82?3>>3:>A>AA>3085<:>:>>5-28<>B>AAA<2>AAA>04	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:1204:4496:9204	147	chrI	4394	60	27S73M	=	3991	-476	TGAATTTTTTTAAAACATTTTTAGTAAAAAATTGTTTTCGTGCAAATTGTCTTCGATATTATCCAAACGTGACGTTTTGCGATTTTCGCGCTAAAATTAC	###########################C>@A?2?;?B@@C>(>C@7;;@@;>?C;@@C;=73@;8FF:=D80)8F:@B;819IHFBD<F8C=,;AA1B?@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:70:7573:20273/1	16	chrI	4573	60	101M	*	0	0	TTTTCATAGTTTTTCTCTTTAAATAAATCACTTTTCAACAAAAAACTATGAGACAATAGTTTGAAATTACAGTATTCTTTAAAGGTGCACGCCTGCTCGAA	CAA>C@EE:EEB?=DCBCC;DED=EDDCEAEECAA?GEAGGGGEEGBEEEGGEGGEGGEGGGEEGGEGGGFFFGGEFGDFGGFGEGGGEGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2315:14704:45455	99	chrI	5186	60	100M	=	5287	201	AACCAGCACCTCTGTTCTCGACCAACTATGATCATCATCGTCGAATAACCGTTTCTCGTGATTTGTCACATTATACTTGAGCACAATACATCCACCAGGT	5;5(@3.@############################################################################################	PG:Z:SNAP	RG:Z:000	NM:i:2
+BS-DSFCONTROL03:121:D0M8KACXX:5:2315:14704:45455	147	chrI	5287	60	100M	=	5186	-201	TCAGTCCTTTCTGAAAATGAAAATTAATTTTAAAAAAATTGAATTATTTTAAATGAAACAGTTTTCAGAGATTTCTCAACTTTTGAGTCCCCCACCAGGC	>8?A@BA:4(>AA>;5;:(:5A>>A@;>>;==6C==?=4HE@.@;FB@=4ECGGFB8=>B9*/910<@>B@>B?39DDCAGEHGC8?<C<)A?:?AD;?=	PG:Z:SNAP	RG:Z:000	NM:i:1
+EAGLE:1:81:1948:19465/1	0	chrI	5499	60	101M	*	0	0	TTGAAAATTAGGGAAATGACCGGACATAAGTTTAGAAACCCGATTTTTTCAATAAAAAGGAAAATTGAAAATTTAATAAAACAGGTTGTAAATCAAGGAGA	FFFGGGGGGFGFEEFGGGGGGGFGGGGGGGADDD:EEEECGGGGFGGGFFEGGFGFEGGGEGFGGFGEGFFEGFDGGFGFE@DGGCFG?EBEBDB?EECEA	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+EAGLE:1:110:7845:14613/1	16	chrI	5562	60	57M1D44M	*	0	0	ATTGAAAATTTAATAAAACAGGTTGTAAATCAAGGAGATCGTATTGATTGAAAAAAATCCGAATGTTCCGGATTTTTCAGTGGTTTTTTTTGAAAGAAAAT	GFEEFFEFDGEGGFBGGFGFGEGGDBGFFGEGGEEGFFEBFDGGEEFDGEGGGGGGG:GGGEGGGDFEFEF=FGGGGGGDGGGGGGGFGDGGGGEGGEGFG	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+EAGLE:1:110:4320:17819/1	0	chrI	7437	60	101M	*	0	0	AATTTCAGTTCAGACCTCATTAAATTTGGGTATATTTCTAGGATCCGAGTTTTTACACCAGATTTACAAACTTTTAGCCTTTCACCGCCTTTTTATGCGCA	GADGGGGFEEGGGGGGDGGGGEGFGGFGFGEGGGFFFFDFGFFEGFFFFDBFFF?BAFFFA5@CCFGADFGGGFBBBFEFGGEEGFFEFEBGGFECAFDD=	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1105:7234:86502	99	chrI	7663	60	100M	=	7927	364	TGGCGTGCGTTGGCAAACAACAAGAAAGAATCATTCTCTGAAAACAAAAAGCGTTTTGGGTGCCAAAGTAATATTGAAAATCTGCCGTGTTTTCTCATTT	@<@FFADDHDHHGIIIIIIIIIIIIIIGIIIGIIIIIIGIIIIIIIIIIIIIGEIEHFHF?DCEDCEC;@ADFEFDACDD@CCDDDDD@8<?AC@CDEEE	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1105:7234:86502	147	chrI	7927	60	100M	=	7663	-364	CAGCTGGTATTATTTCAGACATGGTGCATCGAAAATTCGAAAATTACAGAAATTAACATTTTGGAGCATCTGCCAGAAAATTGAGATTACAGTACCCCAC	CDDDDCDDECCCECCCFEFFFDFHGGHJIIJJJJJIHABHFJJJIIIIGFIJIHF@GIGIHGIGGGFHFJIJIDHIJJJJJIIIHIHF?HHHDDFFF@BB	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1104:4421:72371	99	chrI	8558	60	100M	=	8629	171	GCTCATCAGCCGTAATCAGGTGACCTCATTAGGCCTACCCAAACACAGATTTGTCATTATTTTTCAGACAAAAAACACGAAAAAAATCTTCACGCATGGG	CCCFFFFFHHHHHJJJJJJIHIGHIJJGIJJIJJJJJIJJIJJJJJIIJJIJIJJJJJIJJJJJJIJJJJEHHHFCCDDDDDDDDDDCCDEEDDDDDDDD	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1104:4421:72371	147	chrI	8629	60	100M	=	8558	-171	AAAACACGAAAAAAATCTTCACGCATGGGGTGATAACCTGATTTTAAATCTTACTGTGCCGGCTGGCGCGGCGAGCTTCGATCACTGAGCCGAAAGATTA	?DB?DDBCDBDDDDCDDDDBBDCCCDDDCDEDDDDCDDDDEDDEDDDDCDDDCEEDDFHHJIIGIIJIJIJIIJIIJJIJJJIIJJJHHHGHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:79:1727:12868/1	16	chrI	11973	60	101M	*	0	0	ACGGACCTCTGGGAGTCTGGAAATTCTTGTCTGAAATTATGCTTTTGAATGCTCGAAAGTGGTAAGAATTTAGAATTTATTACAGAAAAACGTTTAATTAA	ABCC>CEEEEEEEEEEEEEEECCDEFFGEEEEEEGEEGGGGGGEEFGFEGGGGFGGEGEFGGFGGGGGGGGGGGGGGGGGGGGDGGGGGGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1315:14029:3494	73	chrI	12099	60	39M1D61M	=	12099	0	AGTATTGTATGCACTGTATCAAAACACATTTTCATCTTTCTAGGTATTCAACTTCACGTTTTTCTGTAATAAATTCTAAATTCTTACCACTTTCGAGCAT	CCBFFFFDHHHHHJIIGIIEIIJJJJ?BFHJJIEIIIJJJJHJJ?DFGHIGGIJJJIIBHIIIIGIJIGHGGHIJJIIGHEIIEGHHHGGHFFEBCCDDD	PG:Z:SNAP	RG:Z:000	NM:i:2
+BS-DSFCONTROL03:121:D0M8KACXX:5:1315:14029:3494	133	chrI	12099	0	*	=	12099	0	AAGTTTTTTTTTCCTTTTTTTTTTGGGACGAAAAAATTTGCAAAAAAATTTTCCCGTGGTGGAGAGTTGAACTCAGACAAAGCCTAAGCCTAGGCTTAAT	CCCDDFFFHHHHGGHIJJFIJJIJBEH=@>AC@DDD<CDACCDDDDDBBCDDDDDBABBABD?ACBAC>CCCDDD@CC<?CCC8>CCCCCCCCCCC<CDA	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:1209:6644:84389	73	chrI	12375	60	100M	=	12375	0	GTACTTTTTAAAGGCACATTGTTTTTTTGGGTCAAGCAAAAATTTGTCGTGTCGAGACCGGCTACGGTATTTTGGCGAAAAATACCAAATTCTTGCGGAT	####################################################################################################	PG:Z:SNAP	RG:Z:000	NM:i:7
+BS-DSFCONTROL03:121:D0M8KACXX:5:1209:6644:84389	133	chrI	12375	0	*	=	12375	0	CCATATGATATCCAAATGAAAAAAATTTTGACTTTACCCCTTATCCTGTTCTACTCGGCCATATAGAGGAAGAAGTATAGCAAAGAAGAAAAACTGGACA	:+=BD,=:C2A4C,++A+3+3++1:CGDB#######################################################################	PG:Z:SNAP	RG:Z:000
+EAGLE:1:37:10171:16610/1	0	chrI	12538	60	101M	*	0	0	TTTGAAATCCAAATTTTTTGGATTGCGAAAAAAACCTGTGTCCGGTTGTTTCATTAGGCCAACAAAGTTCCTGGAACACTGATGAAAACCATGATAGAGGC	GGGDGGFGGGGGAEGGGGGFFFFFBDFAFFEDFEFFFEFDFFFFFEEE?AAECCCFFFFFDFFFFDC@CCFEF:EFD=D?AEEEBEEDFEFBFEFBEDFE5	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:14:9791:11230/1	16	chrI	12732	60	101M	*	0	0	TATGGATATCATATGGATATCAAAATTTTTCTCATATGGATATGGAGAAAATTTTTCTCATATGGACTTTGAAAGTTGAATCACTTGACATCTGGGAAATT	:CDDC=DD?FDGBGEEE=EEEEEEE@GGGBDGDGEFEGFGGGEFGGGGGFECDD?FGFFGGFFFF?FFFFFDDGGFGFGFDGEGGGGEGDGGGGGGEEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2305:19351:44996	99	chrI	13367	60	100M	=	13462	195	GCATAGAAATGATCATTTGGAAATCACGTTTAATTAGGTTACGGCGAAAATTTGCAAAAAAGAGCAGGAAACTTGGCTCAAATCCTTCGAAATATAACAA	CCCFFFFFHHHGHJJJJJIJJJIHIJJJBGICHIIJIJGEHIEIIJJJJJEGIJJJHHHHFBDEEDDBDDDDDACCCDDDDDCCDDDD?B?BDDADECC@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2305:19351:44996	147	chrI	13462	60	100M	=	13367	-195	AACAACTAGGACTTCCATGTAGGCGTTAAAGCGCCCTGTCTCTCACCCCAATCCGTACCTTAAGCTGAAACAAACGTGAACTTTTTTCATTTCTTAAAGG	<>A>:CC@CCACDDDCCDDB?@DDDDCB@@BDDDCFFFDHHHEA;JJIGFHGJIHF?JJJJIIJJIJJHFJGHFJJJJIIJIJIIJJFFHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2203:20505:85498	73	chrI	13826	60	100M	=	13826	0	AAATAATTACATTCAAATTAATATTTTGTTTACGGCTATGGCCTAGAAATCGCGTGGTGGCCTAGGATTCATTTGCGCGCGAAATTCAAATTCAGCCACT	CCCFFFFFHHHHHJJJIJJJIJJJJJJJJJJJJJJJJGJJJBHJJIJJJJJJJJJJJJJJJJHHHHHHFFFFFFEEDDDDDDDDDDCDEDDDDDDDCBDC	PG:Z:SNAP	RG:Z:000	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:2203:20505:85498	133	chrI	13826	0	*	=	13826	0	ATCTTAAAGAAGGAGTATCGTCAATGGGGAAGTTGTTTTAAAATGCAGTATTTGTACTCAAACTTTCAATTATTGCAAAAGAAAAAACGAAAAAAATCCG	CCCFFFFFHHHHHJJHIJJJIJIIIJJJJHGIGHIHIIJJJJJGHIJJIJGGIJJJJJJJJIHIJIJJIJJJJIHFHHEEFFDDDBDDDDB@@BBBDD@9	PG:Z:SNAP	RG:Z:000
+EAGLE:2:66:16575:12892/1	0	chrI	13844	60	101M	*	0	0	CAATATTCTGTTAACGAATATGGCCTAGAAGTTGCGTGGTGGCCTAGGATTCATTTGAGCGCGAAATTCAAATTCAGCCACTTTCGTCGATTTCAACGGCA	GFDGGEBGFGAEGBEGGGGGGGGGGGEGDGGFDGGGGFGBFFDFEGGDD?FGBDFGAGFGGGGEEGGGGGFBDGGCGEGEEEBGDGAB??BBBBB@ADADD	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:2109:19111:33831	73	chrI	13924	60	100M	=	13924	0	CTTTCGTCGATTTCAACGGCTAAATGCGGAATGTTAACGGATTTTTTCCGTTTTTCTTTTGCAATAATTGGAAGTTTGAGTACAAATACTACATTTTAAA	CCCFFFFFHHHHHIJJJJIIIJJJIJIJJGIGIIIIJIJJHJJIJJHHHHHFFFFEEEEEDDEDDDDEED=CDDCDEDDDA@CDDDDDDDDDDDDDEDDA	PG:Z:SNAP	RG:Z:000	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2109:19111:33831	133	chrI	13924	0	*	=	13924	0	AAAGTATAGCACGGCCATGTGTCGATTTACGCAGTTCGTGTACTCCTCGAGGAAAAACTTGGATTTATTTTTAAAAAGGTTATTACAGCTTTAGGTGAAA	@CCFDDFFHHHHHJJJJJJIIHHIGIJJIJGIIJFIJJIJGIJJJJJJJJJJJJHGHHFFFFFFEEEEEEEDDCDCCDDCDDDDEDCDDDDDDDDCCCCC	PG:Z:SNAP	RG:Z:000
+EAGLE:1:88:5129:6620/1	0	chrI	13927	60	101M	*	0	0	TCGTCGATTTCAACGGCAAAATGCTTAATGTTAACGGATTTTTTACGTTTTTCTTTTGCAATAATTGGAAGTTTGAGTACAAATACTACATTTTAAAACAA	FFFBDEAEEEGGFGFDFEFFGDGGGGDBGGGADEEEE:DEEECE:EEBEEBFFACDD@FBEFDEBBD=:EEAEEDDD@DBEEEEFDBBDDED?EBDEEGEE	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:5:16715:13757/1	16	chrI	14162	60	101M	*	0	0	ATGTATTTTAACACAGTTGTGACGTCATAAATGTATTTTAATACATTTTGCAACATTACTTGAATAACCCCATTAAAAATCAACTCAAGCATCAAAAAAAT	:BB?EEEEEBEEEEAE=EEEEDEDEEBAEEEEEEEEEEEEEDBEBEEEE5EEEBE?E=EDEEEDEBADDDAEE?EDEEEEEEEEEEE=EEBEEEEEE?EED	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:28:17761:20206/1	16	chrI	14335	60	101M	*	0	0	CAATTTTTTGTTTTTTGGTCCAACATTTTTTTTTGGTCTCAGCTCTGCTGCCTACCCTAGAAGAACTAATAGCGCTTCAAAAACTGATGAAAACGTTCAAA	C:E?EEC?C9DDFGAEAEEFGEEEEGGCE?FBGGFF=GEFGCEDEE5DCAEFEEFGGGFGGGGGGGEGGGGGGGGGGGGGGGFEFGFGFFGGGGGGGFFFD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2316:19241:55169	165	chrI	15544	0	*	=	15544	0	GGCTTAGACATAGGCTCAGATTTAGGAATCTTCAAAAAATTGAATTCTAGATCTACATTATATTAAGCTGTCTAATTCTCTTCGAAAATTGTCGATTTTT	CCCFFFFFHHHHHJJJIJJJJJIJJJJJJJJJJJJJJJIJJJIJJIJJJJIEIIJJJJJJJIIJJJIGGIJJJIGEHIHHHHHHFFFDEEEEDDBDDDDD	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2116:4460:94851	165	chrI	15544	0	*	=	15544	0	TTTGTACTGTACCGCTACAGTAATCCGTTAGTATCCTAACATCTCTATACTTGAAGTTGAGATGAGTTGTATCTTCACAGACTACAAACTATGAACACAT	@CCFFFFFHHHHHJJJJJJJHHGIJJJIJIJGIIJJJJJJJJJJJJIJJJIIJIJJHIJIJIIJJJHIJIIJJJJHHHHHHFCDFFD>BEEDDDEDDDD:	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2316:19241:55169	89	chrI	15544	60	100M	=	15544	0	AAACTGCGTGGCGTGTACTGCAGAAAACCTCATATTTAGGCCCCGCCTTTTTCTCGACCACTCACGGTGAAAAGGCAAAAATTCGGGGACCAACCAATAT	ADBBBBDBDBBBDDDCADDCDDDBA?9DDEEEDCCDDBDDDDDDFHEIIEIJJJIHFHDBHGGHJJIJJJJJJJJJIJIIIHGIIIJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2116:4460:94851	89	chrI	15544	60	100M	=	15544	0	AAACTGCGTGGCGTGTACTGCAGAAAACCTCATATTTAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATTTGGGGACCAACCAATAT	DD@DDDDDDDDDDDDDDDDDDDDBCB?DDEEEEDDDDDDDDDDHHJJJJJJJJJJJJIHFIIIHIJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:2101:3253:53534	165	chrI	15545	0	*	=	15545	0	GTTGAATGTTGATAGCTCTTCCGAGCGAGTTTTTTTAAATTAAGCTTCATAGCTGTAACATTTAATATGAAGAGTTACACTCAAAATAAAAAAACTGCGT	?@@DDFDDFHDHGGEEFFIHHIGGIIEGBDEHJJIICBFHIDEC;CGDEEDA@EGAEECH;CEEDDEFBC@CCA3>CCDD@CDCDDC>ACDDB#######	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2101:3253:53534	89	chrI	15545	60	100M	=	15545	0	AACTGCGTGGCGTGTACTGCAGAAAACCTCAAATTTAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATCCGGGGACCAACCAATATC	A8DDB@9-?@?:CCCCDCDDDCB@89?DDCAACCC?@DBDDDDFEDEHHBGEIJJIIHEFDIIIIIGIIJJJGFGGGJJHGFJIIJIHHHGHDFFDD@@B	PG:Z:SNAP	RG:Z:000	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2302:10568:99340	73	chrI	15561	60	98M1I1M	=	15561	0	CTGCAGAAAAACTCATATTCAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATTTGGGGACCAACCAATATCAGGTCGCCGACATCTC	=B@FFFFFHH+0AEEEHHAHHHCGHEIDIJGIGIIGGEG=F?HGFHIGIB@AEEHHGFBFDDAC2;>C(9;8;?BB?<<A>@CDA>@4:<50&0>>@@>:	PG:Z:SNAP	RG:Z:000	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2302:10568:99340	133	chrI	15561	0	*	=	15561	0	AGAAAAGGCAAAAATTTGGGGACCAACCAATATCAGGTCGCCGACCTCTCACGGGTTCCGCGCGCCGCTATGTTTAACTCGCAGCGGGCGGAGACAGCTC	A>AAAA@:B@>:BA8298<4(8@??:BEDB>43?5;00B@@;3+8(9?20&<:855'?@>DFIFIIIIFGFB?GFB)D0IFEIEFEEA?FDF?D;DD@@@	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2205:19343:78160	165	chrI	15566	0	*	=	15566	0	TTCTCAACGGTGGTAGAATTTTCACAGAACCGTGCCATGATTCAGATTTTGCAACCCGCGCACAGGTCATGTTTGAACAAATGTTGTCAATCCCTATAAT	CA@??829BCCC@::DDCDDEDCDCC@??BBDDDDEEEECDFDFECHHECHGE@HGGGGGGCCIGGF>IGGJIHGIJGHGHEEIIJIFHHHHFFDDF@CB	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2205:19343:78160	89	chrI	15566	60	64M2D3M1I32M	=	15566	0	GAAAACCTAATATTTAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATTTTGACCCAACCAATATCAGGCCGCCGACATCTCACAGGT	DB@:3DEEDCCDDCD@DDDDB>??5?3C;;@>@CCBC>?BBADCCECEECCFFDEFEHHHHHHGEGIGGBGGIIIIIHGGJIIFJJHDGHGHFFDFFCB@	PG:Z:SNAP	RG:Z:000	NM:i:8
+EAGLE:2:5:10071:18909/1	0	chrI	15576	60	101M	*	0	0	TATTTAGGCCCCGCCTTTTTCTCGTCCACTCACGGAGAAAAGGCAAAAATTTGGGGACCAACCAATATCAGGCCGCCGACATCCTACGGGTTCCGCGCGCC	ECEEEFFFFFGGGGGGGGGGGGGGGGGGGFGGGGGEG?GGGFGGGGFGDBFFGGDGBGFGGGFG:EFDDFGGGGG=BF=FB@:D=BABACBEEGBBEC:E?	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1214:9682:34070	73	chrI	15598	60	61M1I1M37S	=	15598	0	CGTCCACTCACGGAGAAAAGGCAAAAATTTTTACCCCAACCAATATCTGGCCACCGACATCTCACGGGCTCCGCGAGCCGCTGCGTTAAACTCGCTGTGG	@@@FFFFFHHHDHIJJJIJCHGGF@FFGGHIJI<@D=FGGEHGGIIHI>FEFC-@;':B8CDE#####################################	PG:Z:SNAP	RG:Z:000	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:1214:9682:34070	133	chrI	15598	0	*	=	15598	0	AGTTTTTTTTTATTTTGAGTGTACTCCACGAGGATAATTTTTATAATTCTCCTCCTCTTTTCCTCGAGGAGTACACAAACTCCGTGAATCCACACATGAC	##DDDDDDDDDDDDDDCDDDCC@B@BDDDFFFFFFFHHHHHJJJIJIJJJJJJJJJIJJIJJJJJJJIHGIIHCHIJJIJIJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2101:5512:59159	165	chrI	15648	0	*	=	15648	0	GCGTGTACTGCAGAAAACCTAATATTTAGGCCCCGCCTTTTTATCGTCCACTCACGGGGAAAATGCAAAATTCGGGGACCAACCAATATCAGGCCGCCGA	:?BD=0=AFHHGHIIJJJJIJIJJJJJIEHGJJJIIIIIJJJIJJJJJJIHGIIJIJHHFCDEEEDDDDDDDDDDDD@BDDDDDDDDDDEEECDBB@DD#	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2101:5512:59159	89	chrI	15648	60	9S91M	=	15648	0	AATATCAGGCCGCCGACATCCTACGGGTTCCGCGCGCCGCTATGATTAACTCGTTGTGGGCGCGGCAAGCTGTCTCCGCCCGCTGCGAGTTAAACATAGC	#########B>5BDCAC>:B?599>B@93BBBDDDDDDCDDDDDEDC@?DDDDDDDDDDBDDDEDFFFDDEEBIGIIJJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:5
+BS-DSFCONTROL03:121:D0M8KACXX:5:1216:7173:80911	99	chrI	15656	60	96M4S	=	15842	286	ATCCTACGGGTTCCGCGCGCCGCTATGTTTAACTCGCTGTGGGTGTGGCGAGCTGTCTCCGCCCGCTGCGAGTTAAACATAGCGGCGCGCGGAACCCGTG	B@CFFFFFHF@DFHIII1C6DDFHBHGHJHEHIHHCBEFAEFF9AACBBBDB@=A>A>@C<5@B-5>BB9095@>CCC@AC4@B-0559><B3@9@####	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1303:17151:68349	73	chrI	15710	60	68M1I24M7S	=	15710	0	GTCTCCGCCCGCTGCGAGTTAAACATAGCGGCGCGCGGAACACGTAGGATGTCGGCGGCCTGATATTGGTTGGTCCCCGGATTTTTGCCTTTTTTCCGTG	@@?DDFADGHHHDGHIEDCFEEH<DHGGGIBAGHEB??8;@2?:88(23(>@CBB8BB-77?9::>C:(+22;++:?A99-09CC@<@@C:>A#######	PG:Z:SNAP	RG:Z:000	NM:i:6
+BS-DSFCONTROL03:121:D0M8KACXX:5:1303:17151:68349	133	chrI	15710	0	*	=	15710	0	ACACCGCTTGTTTCAGGGCTGTCGTTTTTTCGTTTATTCGGGTTTTTTTTTCGGTTTTTAGAACCAAAAAAACAACAAAAAAGTTTGGTCTGTGTGAAGT	######CDC>>C?<C??<3<99553B;BBBBCDDB?<;BB?;8?8;@AB>EHIGGGGGCHF=8A<7<D?BACGF?G<GHACC?;HGBHFFBA4BFDD<@@	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:1216:7173:80911	147	chrI	15842	60	100M	=	15656	-286	GGTTTTCTGCAGTACACGCCACGCAGTTTTTTTATTTTGAGTGTATAGGTCTCGATTCTCGAAAGTATGACAGTTATTTAAATGATGAACTCGTGATGAC	BDCDDDDDDCDDC>B;DDBBBDDDEDDFHJJJIJGJJJIJJJJIJJIJJJJJJJJIJJJJJJJJJJJJJIJJJJJJIJJJJJJJJJJHHHFHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:82:8204:15172/1	16	chrI	22312	60	101M	*	0	0	GGGGGTACATGGACGAGAATTCTCTACCGTATTCCAATTTGGCTGACTGCGTGCTCAACGTTGAATACTCAGTGTAAACTTTCGTACACCGTTGCGTACTG	E@EE@B@5BBB@@EE?BEB5BEDEEA?CEEE?DBEDEDEDEEE?EE?EEEEEEEEDEBEEBAEEEEBEEBEEEDDEDEDDE?EEEEDCDDDDAD=DC@CCC	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+EAGLE:2:88:6414:9094/1	0	chrI	23853	60	91M1I9M	*	0	0	AAATCGAAAAAAATAATTGCAAAATCCGTCAGCTGAACATTCAAAACTTATCAATTTGAAATCAGCATATTTCAGTGTATAATTAAAAAAGGTTTCAAAAA	GGFEFGEEDDDDEECFFFFFDFDDFFFFFFFEFFFFFFFEFFDFAAEEEEFFFBBDFFFFFF:FDCFF?FBFFEDEFEFFFFFFF?FFFFEA?BEFFFFEB	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+EAGLE:1:88:12428:19742/1	16	chrI	23928	60	1D15M1I85M	*	0	0	GTATAATTAAAAAAGGTTTCAAAAATTCTGAGACCAATTTTTATTGAGAAAAATAATTTTTCGCTCGAATTATTGAATTTTCACTAAATGCAAAAAACAGT	GBD?CGDEEEEFGFEFFD?FFFDFFDDEEAEE=EEEACCFE?GBFFDGDCGFFFBEDGFDAGGA=GFGFBGFFFGGEG?GDGA?DGEDEGEEEFFDEEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:2
+EAGLE:2:39:4472:4563/1	16	chrI	23983	60	101M	*	0	0	ATTTTTCGCTCGAATTATTGAATTTTCACTAAATGCAAAAAACAGTAAACTTGGGCCCATGCTACAAGCCTGAATCTTTCAAATTAAGAACCAGCATGATT	6=CCDDGEEEEEEEGEEFGEEDEE?DEEGDGDBGEEGGGEGDGGGEFFCFEAGGFGGGGEGFGGFGFGGGGGFFDGDFDEGFFFDEGFGGGGGFGEFGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:48:8251:3932/1	16	chrI	23995	60	101M	*	0	0	AATTATTGAATTTTCACTAAATGCAAAAAACAGTAAACTTGGGCCCGTGCTACAAGCCTGAATCTTTCAAATTAAAAACCAGCATGATTTTTTCAATATTC	@DDBD????@==>@>8::FEEEGE@EFGEEGEECACBBCEGEGFGDFFFFBGFEBGFGGGGGFGGGGDBEGEEE5EGEGGGGGGGFGGGFGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:2
+EAGLE:2:96:5841:4603/1	16	chrI	24002	60	101M	*	0	0	GAATTTTCACTAAATGCAAAAAACAGTAAACTTGGGCCCATGCTACAAGCCTGAATCTTTCAAATTAAGAACCAGCATGATTTTTTCAATGTTCTAGGACG	EDBE=EBE?BBEFAED<<<CA??DC???A;ABCBCE5FFEACEC=EEDE=DFGG=GDFFDGEDEDEDDDD5DDDD?:?FFGGFGEFFDFF?FFBEFEFF?E	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+EAGLE:1:51:3584:15408/1	0	chrI	24181	60	101M	*	0	0	AAAAAAATAGTCAAGTTGGGCCCATGCTGTACACCTAAATCATTAAAATTCAGAACCGCCATGTATTTTTTCTTACCAAAGGCTCTTTAAAAAAAATCTGG	=EDEED>AAAEA?BB:DDDADDED?DDDD=DDADDEDEE?EBDEEEEEDDEE5C:EBEE?E=EBBEBE>CEEEAE=?DEDEEBEBDEEE=?BEEACCEEEB	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1209:7257:71539	73	chrII	202	60	100M	=	202	0	TAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGCCCGTTCACCAAAAATAGTGACTCTGACCGTTCACTA	@CCDFFFFHHHGHJJIIIIJJJJJJJIJJFAGGIJJJJJJIIJJJJJJJGIJJJICHIIEIIJJJJGHHFFFFFAA??ACC;ADDDDDCCDCBDDDCDDC	PG:Z:SNAP	RG:Z:000	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:1209:7257:71539	133	chrII	202	0	*	=	202	0	CACTAAATTTGGTGAACGGTCATAGTCACTAAATTTAGTGAACGGTCAGAGTCACTATTTTTGGTGAACGGGCAGAGTCACTAAATTTGGTGAACGGCCA	CCCFFFFFHHGHHHIJIGIFGHHIIHIIJJIJGIJJJJFHIJJJIHIIIHFFGHJIJJJJJJIJHIGIIHHGFDDDDACDDDCDDDEDDDDDDDDBDBB>	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2316:19021:74938	165	chrII	231	0	*	=	231	0	CTAAAAATAGTGACTATGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCG	CCCFFFFFHHFHHJJJIJJJJIHJJJJJJJJJIJJJBBCFHEHDFBHEHGHHIJIACFAHGIFFHI@GDGAEABBDFECEEDD??BDDCDCACCAC5:A<	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2316:19021:74938	89	chrII	231	60	100M	=	231	0	TGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCCGGCCGTTCACTAAAAATAGTGACTATGACCGTTCACCAAAT	CA>DB?BDDBBCCC@<DDCEDEDDDDCC>BAB?BBABCDDB?HHIGGHGHGGHC;GJGGHHIJJGHECAJJJJJIIJJJIHJJJJHFHHHHHFDFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:6
+BS-DSFCONTROL03:121:D0M8KACXX:5:1313:5017:58480	73	chrII	234	60	100M	=	234	0	CTCTGCCCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTTTACCAAAAATAGTGACTCTGACCGTTCACCAAATTTA	CCCFFFFFHHHHHJJJJJJJGIJHIJJJJJJJJJJIJJJJJJJJIIJJJHIJIGJIJIJJJGEEHHDFFFFDEBCEDDCCDCDDDDD@DDDDDDD@CDC@	PG:Z:SNAP	RG:Z:000	NM:i:6
+BS-DSFCONTROL03:121:D0M8KACXX:5:1313:5017:58480	133	chrII	234	0	*	=	234	0	CGGCCAGAGTCACTAAATTTGGTGAACGGTCATAGTCACTAAATTTGGTGAACGGCCAGAGTCACTATTTTTGGTAAACGGCCAGAGTCACTATTTTTGG	@BCFFFFFHHHHHJJJIJJJIJ@FHIJJJEGIGIIGHIJJJCHJJJJIIIFHIJJJJJJJJDHHHHFHGFFFFCDECEDDDBBBDBA:ACDC>A@CDC<9	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2113:7908:63038	73	chrII	234	60	100M	=	234	0	CTCTGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTA	CCCFFFFFHFHHFJIIIJIEHIGEEHGIIIJIJJJJFJIIIBGHIIJIJDDIGJJJJJJJJIIHHFGHHBDDEEEBDEEEDCA@C?@BDDDDDDAACDCC	PG:Z:SNAP	RG:Z:000	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:2113:7908:63038	133	chrII	234	0	*	=	234	0	GTCAGAGTCACTATTTTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGTCACTAAATTTGGTG	BBBFFDFDFFHHHJJJJIJJHIJJJGIIJJIIIFGGHIJJJJIJJJDGIIHJIIGIIHHHHHDDDFDDEECC=ACCCBB8;BDDDACDDCCDDDACACBB	PG:Z:SNAP	RG:Z:000
+EAGLE:1:59:19240:17867/1	16	chrII	234	60	101M	*	0	0	CTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCCGGCCGTTCACCAAAAATAGTGACTATGACCGTTCACCAAATTTAG	=?CCCC:5@?:CA56;7???:EEDFAGEGEGGECFEAFDF=GFGBFGGGEGBGGGGEGGGGG=GGGFEGGGGGGGGGGGGGCCCCC7DDDDFGGGGGGFGG	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+BS-DSFCONTROL03:121:D0M8KACXX:5:1203:6884:43459	73	chrII	238	60	100M	=	238	0	GACCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGA	@CCFFFFFHHHHHJIIJIIAHHIIJJ@HHGIGHIJJJJGIGIJDHIJIJIGGIJJJIIIJEIJIEHIGHHGHFDEEFFACCEDDDCDDDD@DDCD@>@CC	PG:Z:SNAP	RG:Z:000	NM:i:3
+BS-DSFCONTROL03:121:D0M8KACXX:5:1203:6884:43459	133	chrII	238	0	*	=	238	0	GTCAGAGTCACTATATTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGTCACTAAATTTGGTG	?@BDBABAFFHHHJJJJJJJEDHIJJGIJJHGICGHIJJJJGIIJJIGIJJIJJJJGIJHHEEHEE@@CFEF=AC>CDDDDD??B:@ACAACDAAAAC??	PG:Z:SNAP	RG:Z:000
+EAGLE:1:50:4361:1823/1	0	chrII	240	60	101M	*	0	0	CCGTTCACCAAAAGTAGTGACTCTGACCGTTCACCAAAAAGAGTGACTCTGACCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGACTC	GGEGGGGGGGGGGGGGGGGGDAFFFDFFFFGGCGFEGEGGFC@ABFEEFEGEGEFCFGAEEEDE:DD@>CC5@ACAC:CCC:C==?A?@=:;;;=@CA?@5	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:1:85:17281:13087/1	16	chrII	240	60	101M	*	0	0	CCGTTCACCAAAAATAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTC	==<76>CF@CAGEDEFEEC?EED?CDBCB:=EDEGGGGCDEFFEE?FFFDFDGGFFFGEFFGFEDFFGGDGGGGEGGGGEGDDGGGGGGFDGEGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:78:10927:5210/1	16	chrII	240	60	101M	*	0	0	CCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGACTC	F@?@:B;EEEE:D?EEDEEAFEFEEEFFDCEGEDAEFAFDFEDFFDFFFFGGFGGBGGGDGGGGGGGGGGGEGFFGGGGGGGGGGBGDGGFGGGGDGGEGG	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:1116:20571:27060	165	chrII	241	0	*	=	241	0	AATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTCAGTGACTCTGGCCGTTCACCAAATTTAGTTACTCTGGTCGTTC	@@BFFFFDHHDHGIJJJIIJJJJJJJJJJGHGIHGIJJJIIIJAHIJJJJJIJJJJIGGHIDAHIAHIIHFDDFFEEEBCCDCDDDCDCCCDCDCC==<A	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:1116:20571:27060	89	chrII	241	60	100M	=	241	0	CGTTCACCAAATTTAGTGACTCTGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGATCGTTCACCAAATTTAGTGACTC	8CDDBBDDCD@5:DCA@@;;CCDBAADED@?EHCEFGHJJIGHEFJJIGHIJIFDIHGIHJIIEGIIIHGFJJJIGIGIHEHGGJIJHHHHHFFFFF@@C	PG:Z:SNAP	RG:Z:000	NM:i:8
+EAGLE:2:77:9514:17665/1	16	chrII	241	60	101M	*	0	0	CGTTCACCAAAATTAGTGACTCTGGCCGTTCACCAAATGTAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGGCCGTTCACCAAATATAGTGACTCT	EBB@B?DDDDDBBE=EBB5??B?EEDEEBBEBBEBACC?ACDDD@DEEEDEEDEDCEDEEEEEEEEEEEEBDADDDDDDDBDEE:EDFDFFEEDDECEDBE	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:97:16219:20975/1	0	chrII	244	60	101M	*	0	0	TCACCAAAAATAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGC	CC=CC?DBDB=@@?D:?AA?CD:BBABA?DBE?BEEB>???@>>>@?:EBB?:B:B@EEBBEA?B@?@>8@@>@@;?<??@@?<?8???<=?7?@=<<>>?	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:55:11493:15098/1	0	chrII	245	60	101M	*	0	0	CACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGGCCGTTCACCAAATATAGTGACTCTGGCC	5FFFFFFFD?ADBDDFFFAE=?DDBFEFFFAD?F;DDDADEEEEEFABC=B?EEEFDBFE??DAA:?@=B?C=@B>5=@@;8;87>>=>>B?5BAB6@::9	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:61:8991:6659/1	0	chrII	246	60	101M	*	0	0	ACCAAATATAGTGACTCTGACCGTTCACCAAGTATAGTGACTCTGGCCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGGCCG	FGFGGGGAGGEDGGGGGGEGGGGGFFAFFFFF:FAD=@CDEEE=EFFED=AAAABFBG:BBBEAEEAEFE=?EEE=>=>>GDDEDDB@D@=A@@?A:BBB9	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:1:73:10783:17479/1	0	chrII	249	60	101M	*	0	0	AAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGGCCGTTCACCAAATATAGTGACTCTGGCCGTTC	GEGGEGDGGGAFFFFGDGGFEGGGGGGGGGFFGFEFFGGGFFGGGDBGGGGGGEFDGBGEBEBFDFCD?ECECDCBDFDEEED?EC<BCCAC?CCACAA=C	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:47:10664:4304/1	0	chrII	249	60	101M	*	0	0	AAAAATAGTGACTCTGGCCATTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGGCCGTTC	FGGGGGFGBGDGGGGGGGGFGGGGGGGEGGGGGGEGGGGGGGDGGGEGGFGEGGGGGGDGEGDEGGFGEGGGCEEGEGEEEEEEEEEEDEEEEEEEEE?EC	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:84:13989:21133/1	0	chrII	251	60	101M	*	0	0	AAATAGTGACTCTGGCCATTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGACCGTTCACCAAATATAGTGACTCTGACCGTTCAC	GGGAGGGGBGBFFFFGGGGGGGGGGFGFFGFGEGDGFGGEGGGGEEGEGGFGFEGEDEEEDGEEEEEEGEEEEAEEEEEEBA?A@CACACEB?EEA?AC?C	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:46:5068:2631/1	16	chrII	254	60	101M	*	0	0	AAGTGACTCTGACCGTTCACCAAAATTAGTGACTCTGGCCGTTCACCAAAATTAGTGACTCTGGCCGTTTACCAAATAAAGTGACTCTGACCGTTCACCAA	CCCBC?C?C?@:CC=?::BDCDFD?EDFF=EFFFFDGEGGEG?GDGDFFF?B?FFFEDGDGGGFGGEEDBBFEEFFFGGGGDFFFFGFFGGFFAFFDCEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+EAGLE:2:14:17135:3972/1	16	chrII	259	60	101M	*	0	0	ACTCTGGCCGTTCACCAAATTCAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGATCGTTCACCAAATTTAGTGACTCTGACCGTTCACCAAAAATT	5A?BA=@CCBA5ECEEABCC=DFFBE?GFGFEFFGGDFGEDGGGG?FGEGBGDGGGFGGDGGGGGGGFGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+EAGLE:2:50:3266:18098/1	16	chrII	264	60	101M	*	0	0	GGCCGTTCACCAAATTTAGTGACTCTGACCGTTCACCAAAAATAGTGACTCTGACCGTTCACCAAATTTAGTGACTCTGGCCGTTCACCAAAAATTGTGAC	B;????BC@C?B:@?B@EB@E?EBEEE@DEEEEC:E78A@5D?CECE@EBECD;?DBEBD?EEEEEFBEFFAFEFDFFFEEEEAAE?EEE=BEEEADDDDB	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:51:8846:13213/1	0	chrII	675	60	101M	*	0	0	TTACCGCCACCGATGTGCCCGACGCGACTTACTGTTAGCCCTTGTTTTGCACAAATCTGTTGGCTTCCATATTTAAAAGTTAATTAATGACCCAATGTTCT	A<6?;@>@BBEEEEEEEEEEEEAEEE?E?AEEEEEBDEA?C@>@=EB<8BEEBDDBDBC???CC5@B??@?EBEBEDEE@A:@?B:?=@=@:B@B@???BB	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1316:6280:52543	99	chrII	1371	60	100M	=	1443	172	GCTCAGGACACCTCATACAACTCCAGAGAAAATGTGTCTCATTATTCTTGTCTTTTTTCAAGATCTAATCAATTTTCTACATTAACGACGTTTTTGTCGT	CCCFFDDDHHHGFGHGGJIJGGGGGIHIGHIJIIJI?FHJIJIJJJIIEIGEGGEHIIJAFC@CHHG>EHH@CEFHHHCDE@C@3;A59;8<CBB@B@0<	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1316:6280:52543	147	chrII	1443	60	100M	=	1371	-172	TTTTCTACATTAACGACGTTTTTGTCGTTCTGCTTCTTTTTTTCGTTCGTTTGTCTCGTCCATCAGCTGTCCACTCATTTCTCTCCCACTCACTAGGCAG	9CAC@@:CA?DB@@<?BDBDCBB@B>DDC?>:>9DBBBEFEDFEGIIFJJIHA:IJJJIIGF>GIIGIGIHFC@FFGFJIHDGH?GHFHHFFFDDFFCC@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1302:4189:8113	99	chrII	2989	60	100M	=	3262	369	AGTATGGACAATTGTGAACGGAGAGGTTCAATGGAAGACTCCACCGCGGTAAGTGTGTTTCTTTAAAAATTACTTCCTTTTTTCAATTGTTTGAAATTAA	CC@FFEFFGHHGHJEIGIJJJFHAHICGFGIIGIGHGGGHHFHIIJJIJ4@EGEDAEECHHFFFEBDFEEEEEDDDDDDDC@BCCDEDDDDC?CCCCDA@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1113:11974:31213	99	chrII	3014	60	100M	=	3127	213	GTTCAATGGAAGACTCCACCGCGGTAAGTGTGTTTCTTTAAAAATTACTTCCTTTTTTCAATTGTTTGAAATTAACAAGAAACCTGTTGGAGCGTATTTC	CCCFFFFFHHHHHJJJJJJJJIJJEHIJBGGHHIIIIJJEHIIFIJIJIIJJJJJJJJHHFHHHHFFFFFEEEEEECDDDDDDDDDDDDDDDDD9BDDED	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1113:11974:31213	147	chrII	3127	60	100M	=	3014	-213	CGAAAATATCATTTGCAAAAAAACTTGAAAATTGAGAAACTTTTTTAAAAGTGGAGTAGCGTCTGCGGGTTTTTTTGCCCTAAATGACAGAATACATACC	BEEEEEDCDDCDDDCDDDBDDA>DDDEDDEDDEDDEEDDDDFFFFFFHFHHFJJIJJJJJJJJJJJJJJJJJIJJIFJJIIIJJJJJHHHHHFFFFD@CB	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1302:4189:8113	147	chrII	3262	60	4S96M	=	2989	-369	TGCGATTTTTATTTTTATTTTTCATGAATGTTAGGGGCAAAAAACCCACATGCGCTACTCCGCCTTAAGAAGAATCAGCTGTGAGCACTATCCACTATAC	####?CCC@C?:@DCDBBCC@>CCDDCC@A<<?DC>A;?BBB;5EEECIHBA@9ED?@0FDBGFIEHIIJIGJHGJJIJJJJIJIHHDHGHHFFFFF@BB	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:87:12598:13225/1	16	chrII	4380	60	101M	*	0	0	AAATTCATGAAAACTTTACTGAAAAACGAAGACCCATTCGATCATGCAAACAAAATATAAGTTTCTATGAAATGGACGGGGATATAGAAGAATTTGAAGTG	DAAA?CCDACC?=?CAB<A@ACCC=C=@B@?:CCEEE5FGFFFGGGGGEGDGGGGFGGGGGGGGGEGFGGGFFGGGGGGGGGGGGGGGGGGGGFGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:82:12769:6412/1	16	chrII	5968	60	101M	*	0	0	TAAAATATCAGTACACTCCTGGTAGAACAAATATTTTATTTCAATGTTTATGTATGAGTAGAAAAGTTAAACAGAACTATTATTTGGCATCAATTTCTGTT	GEGEGEEDFEGGEGEGEFGGGGGFGEEGGBGFGGEEGFGGFGFGFGGGGGFGGGFGGGGGGFGGGGGGGGGGGGGGGFGGGF?GGGGGGBGGGGDGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2303:1316:84598	99	chrII	7139	60	100M	=	7167	128	CATCTACCGTATATTCTTTACTAGTGCTGCAGGCAGCACTAATTTCTAGGCCCTTTTTTAATGCAGTACTATTAGAGACTGCAGTACTACTGGAGATGCA	@C@FFFFFHFFHHJIGIJJIJIJI4BFIEHCGHFGGDHGIGGGIGGIIBGGDHIDHIIIGIIDHIIJFEHHHHECC@DFBEDDD>CCEACD@C?<CCC3:	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2303:1316:84598	147	chrII	7167	60	100M	=	7139	-128	GCAGGCAGCACTAATTTCTAGGCCCTTTTTTAATGCAGTACTATTAGAGACTGCAGTACTACTGGAGATGCAGCGCTAATAGAGAATATACGGTATGTAG	DCC<CA>3CDCDCACC@;EC?=<FEGIGGGGIGIGIHG@JGGIHFCIHIGHGIHGDFIHEDHGJJIJIJJJIIIEEJIIJFAHDGEIFFDHFFFDDB@@@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:25:17975:18268/1	0	chrII	7306	60	101M	*	0	0	CTTAGAAAGGCTAATATTATTTTTAAAATCAAGATTTAAGTTAATTAACACGACCTCGTATTCTATATTTCAGATATGTCAGTTATGCATGTGTCATGTTT	GGGEFGGDGGGGGFEGGGFBFGGGGGGGEGFGGGFFGFDEEGGGE=FFFFEEAAECE?CCEEGEEE@DADEEEGB?:CACFEACECEFBCDBEBECDEC:?	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1104:15150:63415	99	chrII	7998	60	100M	=	8021	123	GTCTATGGAACAAGAAGGAAGACGAGAAAGCGTGTAAGACCATGAAATGTTTTTTGTGTGCGCAACGTAGTGCGAAAAGTTAGTTATTTGGAGAGGAAGT	C@@FFFFFFHHHGGGGGIJJIJIJJIJJJIIIIJIJIIGIJJJJJIJJJJFGHIJJHHEEFFDACBDBBB@CCDB=@BC:@D@@CDDDEDC>ACDDDDBD	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1104:15150:63415	147	chrII	8021	60	100M	=	7998	-123	GAGAAAGCGTGTAAGACCATGAAATGTTTTTTGTGTGCGCAACGTAGTGCGAAAAGTTAGTTATTTGGAGAGGAAGTTATTTGAAAAGAATCTTTAGAAG	CCCBBDD@DDDEDCDCDDEEEDACBDDDDFFFHHHGIIGIHDIIJJIGIIGHJJHIIIJJJJJJIIJIGHFJIHEIJIJJJJJJJIHBHHGHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2314:19499:38834	99	chrII	8057	60	100M	=	8275	318	GCGCAACGTAGTGCGAAAAGTTAGTTATTTGGAGAGGAAGTTATTTGAAAAGAATCTTTAGAAGATATTGATATTTTATATTCTGTATGTAGGCGCTGTG	7BBFFFDFHDFDHEHGGIAHGHIJDHIIJJJJBGFHI;DHBGIJJJGHIJIIJIIJJJJII)=EHFHHFFFFFFFFEFDEEEEDEDDCDCDCAA?@BDBC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2314:19499:38834	147	chrII	8275	60	100M	=	8057	-318	GTTCGTATTCTCTCAACTCATGGCACTACTCTCGATGTGCATTGCCTTTTATGGTTGCTACGTTATGGTTCCTCTTGGAAGAGAAAAGCATGCTCCATAC	BDDDDDDDDDDDDDDCCEEEEECCFEB;FHHHHGDGJIJJIJHGIIJJJJIHCJIHGHHFJIJJIJIJIIFIIIJJJJJJIIHJIJJHHFHGFFFFF@BB	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:79:2735:13063/1	0	chrII	9267	60	101M	*	0	0	TACACTATTCTTAACTTTTTGACATGAATTAAATCTTTTTAAACTGAGTCTAAGCGTATTATTGTATCGTATTTTCCCCTTCTGATGTATTCATTTACTTG	ADEBED=ADC=C6=CD=DD6C5BA>A?DDDEBE=AACCA<@B:EEEAEB?ED=EEA<AABCCA@:BB?B@5ACCCEBDEDEBBE:5??:?=@:?@E?BCBC	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2114:17065:72325	99	chrII	9394	60	100M	=	9559	265	CAACTACCCAATTTTCCTGTGCCATGTTTCTTGAAACAAATCAAATGTGATAAATAGTTTGAATGCCTTTATGTATAATAAAATCAATTTTTCAAGCCGG	CCCFFFFFHHFHHJJJJBIFGHJJJJIJJJIJJJJIJJJJJJJGGIIFIIHIJJJJJGIIIJIJJJJJJJIJJIJIJIJJJJFIHHHHHHFDFFBECCC>	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2114:17065:72325	147	chrII	9559	60	100M	=	9394	-265	ATGAAAAGCCTGTTCTTTGGTGTTTAAAGGTAGCACACAAACCAAAATTTGTTAGCGCAAATTTAAATTTCTATTTCAGTTACCATGAGTTAGTCTAACA	@DDDDDB?@DDDBDDDDDDDFFFFFFHHHHHJJIHGGJJJHJJJJJIGIHJIIJJJJJJJJJJIJJJJJIEIJIJIJIJJHFJIIIJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:91:5280:21105/1	16	chrII	10935	60	101M	*	0	0	CAATGCCAATACAAAATAAGTCCGAACAGCCAGGTTAACAACTTGAGTATAAACCAGAGGAATCGGCACCATATCGAAAATGACCAGATTTAGAATTTTTG	8==?=AAA??589??A8A5A?BAA?>D?ADAABABADAEE?EBDDBEBEGEBBFFD=:?;5ABEBEGEFFEGEBDGFEFFGGBGGEGGFFEGGFFFGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2209:12690:32871	99	chrII	11660	60	100M	=	11873	313	TTGTTAAGCAATATTCTATAAATCACTGATAAAATAGAATAACACAAAAGCCAAATTAGTAACTCTGACCATATTGATTTCCAAACCGATCCTTTCCATC	CC@FFFFFGHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIIHHHJIJJJJHGIIIJIIIIJJJJJIHGIJJJGGHHH=BFDDDDDDDDDD@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2209:12690:32871	147	chrII	11873	60	100M	=	11660	-313	TTGTATCAGTCGGTGGGTGAGCGATTAGTATCAAGTAGCACAAACGCTTCCGTTTCAGATTTGCATATTTTATTGTTATAGGGGTTATTCAGGCATAGGT	DDDDDDDDDDDDDDDDDDCEDFFFFFFHHHHHHIIIJJJJJJIJIIHG@JJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:1
+EAGLE:2:118:13551:9082/1	16	chrII	13278	60	101M	*	0	0	TGATACTTATTCCAATTTCATTCCAATTACATTCATGCTTGGATTTTATGTCTCTGCTGTTTTCACACGATGGTGGCAGATTTTCGACAACATAGGGTGGA	EBCDADACBC:EFDEBDFEBEDEBEF=EBFEEBFDEEFEF?FFDFFAAFEFEDGFDGFGGFEEE?EEGEGGGEGGGGGGFGGGBBGFGDGEGGGGGGFGGF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2107:16551:14335	99	chrII	13632	60	100M	=	13690	158	TTTAGTTACTGCTGGCTTGATGACAGAAAAAGAAATGGCCGAGTTCGAGTCTATCCCTAGTCCACACGCAAAATATTGGCAGCCAATGCATTGGTTGTTT	CCCFFFFFHHHHHJJJJJJJJIIJJJIJJJJGGIJGIJIJJGIFHIJJJGHIJJIJJIJJHIJHHHHFFDDDDCDDDDDDDDBDBCCDCCDDCDCDD9BA	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2107:16551:14335	147	chrII	13690	60	100M	=	13632	-158	AGTCCACACGCAAAATATTGGCAGCCAATGCATTGGTTGTTTTCGATGATCACTTTAGCGCGAGACGAAGGAATGATTTCAAGTGATATCATATATGTAG	CCCDDBB?DDDEEFDDCDDDDDEEEEEFFFFFHHHHJJJJJJJJJJJIIGHFJIJJJJJJJJJJIJJJJJJJIJJJJJJJJJJIJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:47:2491:18712/1	16	chrII	14238	60	101M	*	0	0	GAAAAATTTGATTATTTAATTCAATTTTAAGTTAGAAAAATATCCTACACATTTTCTGAAGAAGTGTCGTAAATGGGGAAACTTTTTAAACATGTACGTTC	EEEBCEAGEGEGBFEFFEDEDDEFGDEGDGGGGEGEGDFFCEFFFFDFDFFEBEEFBFGFGGGGGGGGGGGGGGGGGGDGEGEGGGEEGGGGDGFAEEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:57:14985:11088/1	0	chrII	15505	60	101M	*	0	0	ACTTTAACTCTCCTGTCAAATACAACACTGATGGGATGAAAGACCGAGAGCTTCAAAATCCAACACCAATCACTGATCACATTGATTTGCCTTTGCATGTG	FFFFFFADFFDAA?DEEBEEDFFFFFDFFFFFFFF?EEEEFFFFFF=F?BFBEDDBBCBBFEFEDFD?=E@@E@B:=??CDD:EDDBB5>>=5=>B??B?C	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:21:7517:15303/1	16	chrII	15804	60	101M	*	0	0	TTCAGGTAGTGATCTAGGCAAGTCTGGAAAGCGGGAGAGAAAGAAGAGCGAGTGATTTTTGAACAGTATGATAAAATATTTTTTGTTTCTCTTTTCACTCT	E5AFEBAFDEDEFFCEAEC?ED?DAEFFCF?FFFFFFBFFFFDDFEDDDD?FADFFF=DFFFFFFEEF=FFFFFFFFFEFFC>FFFAEGFEFDBDFBDDEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1212:2979:94901	99	chrII	16035	60	100M	=	16399	464	GTTCTATAGGACCCCCCCTAATTTGAAGGTTTGAGGAACTTCCGAAAATTTTTTTGAAAAACTGCTAATGCCATTCGTTTTTAAATTGAAAAAAACCTAT	CCCFFFFFHHHHHJJJJJJJJJJJJJIJJHIJJHIJIIJJJJJJJJJHHHHHHDBDDDDDDDDCDDDDDDDDDDDCDCCDBDDDDEECCDDCBDDDD?C@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2310:11814:52578	99	chrII	16055	60	100M	=	16433	478	ATTTGAAGGTTTGAGGAACTTCCGAAAATTTTTTTGAAAAACTGCTAATGCCATTCGTTTTTAAATTGAAAAAAACCTATATACATTTTTTTCCAGAAGT	1=BD=2=ADCFHHGJJJJJJJJJJJJJJJIJIJJJIJJJIIIJJJIJIJJHIJJJHHGHHFFFFFDEEDEDCBBDD@CCDDDEDEEEEDDDDDDECCCDC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:74:13479:19610/1	16	chrII	16067	60	101M	*	0	0	GAGGAACTTCCGAAAATTTTTTTGAAAAACTGCTAATGCCATTCGTTTTTAAATTGAAAAAAACCTATATACATTTTTTTCCAGAAGTTTATCTCAAAAAC	EEEFBEECBDDEEEEEEEEFEEEEEEEEEGEGGGFEEGFEFBEFGFGEFGGGGGGGGGGGEECEE?GGGGEGGFEGCCDADD:DDDGGGGGFGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:60:16490:10079/1	0	chrII	16083	60	101M	*	0	0	TTTTTTTGAAAAACTGCTAATGCCATTCGTTTTTAAATTGAAAAAAACCTATATACATTTTTTTCCAGAAGTTTATCTCAAAAACTGAGGTCGCGCTGGAA	GGGGGGGGGGGEGGGGGGGGFGGGGGGGGBGGFGFFGGGFGGGGGGGGGGGFDFGGFGFGGGG8GGGEEGGC?FGDGDGGGEGGGEGEGGBDEGEBAECCG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1313:14692:64885	99	chrII	16208	60	100M	=	16423	315	CTTCTATAGGACCCCCCGTTTTTTTTCACGATTTTTACTAAAATCAACAGATTTTGGAATTTTTGACAAAGCTCAAATCAAGTTTGAGTTAGAAATGAGT	CCCFFFFFHHHHHJJJJIIJIJJJJJHJJJIJJJJJIJIHHHHHHFFFFFCEEEEEBDDDDDDCB@DCCCDDDDDDDDDDDDDDEDDDDEDDDDCDD>CD	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1212:2979:94901	147	chrII	16399	60	100M	=	16035	-464	AGTCTGTTTCAAGCTTCCTGGTGCTCTCGGTAATGCCAAAACTTGATAAACTCTCTTTAACAAGTTCCTACTAAAATTCCTAGCACACACACCATAAACA	ABDDDDDCBCDCDDDDDDEDCFFFGGHGIJJIIEIGGCJHDB@IIGIHEGIGIIIIJIJIIGGHCIHCIHJJJJJJIJJIGIGGEIHHFFCHFFEFDB@@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:79:2445:14024/1	16	chrII	16410	60	101M	*	0	0	AGCTTCCTGGTGCTCTCGGTAATGCCAAAACTTGATAAACTCTCTTTAACAAGTTCCTACTAAAATTCCTAGCACACACACCATAAACATTTTTACGCCAT	CACAACF=CCDEG@GEFDEAEE:EEFG5EEGEFEFEGFEGEGEFFEGGGGGGF=EGGEEGEDGGFFEFFBFFFFFFEEEAEGGBGDEGGGGGFAGGGFFEF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1313:14692:64885	147	chrII	16423	60	100M	=	16208	-315	TCTCGGTAATGCCAAAACTTGATAAACTCTCTTTAACAAGTTCCTACTAAAATTCCTAGCACACACACCATAAACATTTTTACGCCATCCCCAAGAAACC	>DDDCDDDDDDDDDEDEEEEEEECB?B>FHHEHGHGIJJIHGJJIHIGJJIIIIJJJIHHDIIIHHGJIGEJJIIJJIJJJIJJJJJHHHHHFFFFDBCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2310:11814:52578	147	chrII	16433	60	100M	=	16055	-478	GCCAAAACTTGATAAACTCTCTTTAACAAGTTCCTACTAAAATTCCTAGCACACACACCATAAACATTTTTACGCCATCCCCAAGAAACCAGTCAGAAAC	CDDDCDC@:@DCDAA>DCEDCDED@CBCEHCBHHE@EBIJHFAGHGHEEC;ECGFFDJIJIJIIIEIGGHHEIGIHGEJJJJJJJIHFHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:84:7059:2112/1	0	chrII	16465	60	101M	*	0	0	CCTACTAAAATTCCTAGCACACACACCATAAACATTTTTACGCCATCCCCAAGAAACCAGTCAGAAACAGCGTATTAACAAGTTGCAGTTATTTTTGATCA	GGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGFGGGGGEGCGGGFGGGGFDGEGGEGGEEGGGEEEEEEDFCD@EEADDEEEEBECE>C@BCC<E=EBEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:23:16149:11703/1	0	chrII	16484	60	101M	*	0	0	CACACACCATAAACATTTTTACGCCATCCCCAAGAAACCAGTCAGAAACAGCGTATTAACAAGTTGCAGTTATTTTTGATCAACAACAGAACATTCATATA	EEEEE:E?D??DDCADBEEEBEBDBBDBED:DDD5;A?A5C?CC@BEEEEBACBB?5BCEBE=BEEEBC=:@:8<:?5B?E=BB?:BBBBADAA5BE?B=D	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:90:13478:14626/1	16	chrII	16489	60	101M	*	0	0	ACCATAAACATTTTTACGCCATCCCCAAGAAACCAGTCAGAAACAGCGTATTAACAAGTTGCAGTTATTTTTGATCAACAACAGAACATTCATATACTAAA	:CDD@CEAEEEDC@BCFDEBC=FBFDEGEEEEEGFFEGEGFGEGGGGGGGGGGEGGGFGGGGGGEGFGGGGGGGGGGGGGGGEGGGFGGGFGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:111:13020:19687/1	16	chrII	16513	60	101M	*	0	0	CCAAGAAACCAGTCAGAAACAGCGTATTAACAAGTTGCAGTTATTTTTGATCAACAACAGAACATTCATATACTAAAATCAAGAAAGGATCAATAGTTAAT	EEC?=ECCBGEBEAFEBEEGGEGEEF=EE5EGEEGGEGGE?EGEGGC=GGEFEFAGGGGGGEGGDDFGGFFBBFGGGGFGGGGGGDGFGFGFFFFFEE=EE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1314:6052:32630	99	chrII	16537	60	100M	=	16752	315	TATTAACAAGTTGCAGTTATTTTTGATCAACAACAGAACATTCATATACTAAAATCAAGAAAGGATCAATAGTTAATCGGGTTTCCTTGTGTGCGGATGA	@BCFFFFFHHHHHJJJJJJJJJJJJJIJJJJJJJJJJJJJJIJJIJJJJJJIIJJJJJJIJJJJIJIJJJJJGIIIJHHHFBDDCEEEEDDDDCDDDBDC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1104:21126:70246	99	chrII	16655	60	76M24S	=	16691	101	CCATTGATCTGACCAAACTTTTCAGCTGGTTGTCCGGAATAGACTTCCAAGCGTCGAGAATTCCTTGCTTCAACGATGCAACTGTTGGGTAAGTCTTGTT	+=?DDF;?CCFFF<EEF@FI>FDCFEHIIBDEI99:C06DB<4?BBBB<@?67B=AAHG/AAC:?DFDDF(66>>@########################	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1104:21126:70246	147	chrII	16691	60	35S65M	=	16655	-101	CATTGATCTGACCAAACTTTTCAGCTGGTTGTCCGGAATAGACTTCCAAGCGTCGAGAATTCCTTGCTTCAACGATGCAACTGTTGGGTAAGTCTTGTTC	###################################?C=)?7).):;@CAA@<D?DDBB90)6?BDC9C8;EE:<EAD@FF::?EC3ED:<+:D:D:;?=?	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1314:6052:32630	147	chrII	16752	60	100M	=	16537	-315	GTTCTGAGCATACACGATACGGACAAGAATCCCCCACAAATTTTCGATTGGATTGAGATCAGGACTTCGAGCTGGCCAATCAAGAAGGTTGATCTTCTTG	DCDDCDEDEDDDDDDBDDDDDDDDDDDDDDDFHGGDJIJJJIIJJJJIJJJJJJJJJJJJJJJIJJJJJJJJIJJJJJJJJJJJJIIHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2309:5944:13872	99	chrII	16838	60	100M	=	16910	172	GGTTGATCTTCTTGAGCTTGAAATAGTCGCGGGTTGAGTTGCTCACATGGATTGTCGCATTATCCTGCTGAAATCTAAAGTTTTTTCTGGAGTAGTGACG	BCBDDDEFHHHHHJIJJJJJJJJIIIIGGIJJJDGGGGCGGGIIIJJJJJHHHHHGFFFDDDDEDEDDDDCDDDDCCDDDDDEDDDCDDCCDACDDDEDD	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1312:16439:9749	99	chrII	16889	60	100M	=	17198	409	TTGTCGCATTATCCTGCTGAAATCTAAAGTCTTTTCTGGAGTAGTGACGAAGATATTTGGAGAGCTCCAGTTCCAAGACGTTCTGATAGTCAGTGCTGTT	CC@DFFFFHHHHHJJJJIJGJGIIIJJJJDCHIJJJJJJGIHGGHHJJIJHIJJJJIIIIJJIJJIJJJHHHGHHHFFFFDDEDDEEDDCDEDDDDEDC@	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:2309:5944:13872	147	chrII	16910	60	100M	=	16838	-172	ATCTAAAGTTTTTTCTGGAGTAGTGACGAAGATATTTGGAGAGCTCCAGTTCCAAGACGTTCTGATAGTCAGTGCTGTTCATCTTGCTACTGACGAACTG	CCDDDDDBBDDDDDDEEEEEEFFFFHHHHHHJJJJJJJJJJJJJIJJJJHJJJJJIHJJJJIJJJJJJJJJJJIJJIJIJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2310:10135:61245	99	chrII	16930	60	100M	=	17321	491	TAGTGACGAAGATATTTGGAGAGCTCCAGTTCCAAGACGTTCTGATAGTCAGTGCTGTTCATCTTGCTACTGACGAACTGTATCTCAAGCTTCTTCTTCT	@@@FDFDFFFHHDBFHIBFG@@BGEGHGIGHEHICHGIGIJEGHGIIJGGFHIDEGHFEHIJHI;F>EHIJCEHBHFFBC3;ACCECCCCC@CDACACD>	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:75:15288:18925/1	16	chrII	16992	60	101M	*	0	0	CTTGCTACTGACGAACTGTATCTCAAGCTTCTTCTTCTCCGTGAACGCTCCCCAAACCATCACCGTTCCTCCTCCAAAATTACGTCTCGAAAAAACCATTG	CC@CDD?DDDAEEEEEEDDBEFDEAEEGBEGEEGEEFDGGEGFGEGFGFFFFFFFAEEEEECGFGGEGGGGDFGGFGFGGGGGGBGDGGGGFGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:48:9783:12656/1	16	chrII	16993	60	101M	*	0	0	TTGCTACTGACGAACTGTATCTCAAGCTTCTTCTTCTCCGTGAACGCTCCCCAAACCATCACCGTTCCTCCTCCAAAATTACGTCTCGAAAAAACCATTGG	=???=7A???A5==>:A??=A5BA?@BBB?B:BAAA5?A>:BA=BBBABBD?B?BACBCDBDCDCBDDBCDBDDDDD?DDBDD?DC>>B==DD=ADDD:DD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1312:16439:9749	147	chrII	17198	60	100M	=	16889	-409	CACTCTTGCTTTTTTAAATTCTCACTTTACTCCAATTCGTTCCCATATTGTTCTTAGCAAATTCCAATCGCTTGAGTTTATGGTCTGCAGAGAGTAACGG	4@DDDDDDDDCDDEDCEEDCCD@;@CDDEHHFHFIIIJIGIJIJJJJJIJIHIJJIJJIJJJIJJIIGJIJJJJJJJJIIJJJJIJJHHHHHFFFFDCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2310:10135:61245	147	chrII	17321	60	100M	=	16930	-491	CGATTACACCAGATCGTTTGATGACATTGAGGATGGTCCTTTTTGAAGCAGACAATTGAAGCTCATTGCGAATATCTCTTGCCGTCTTACAGGAGTTGGA	0:@@@AA:CCB@DB@DDCCDDEDCCCEEFFFFDHHHHEBIJIHJIIGIGEGAGEJJIIJJIHGCHBGHGIGIIIJIIIIJIGJIJGGFHHHHFFFDF@B@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1301:10889:93999	99	chrII	17346	60	99M1S	=	17605	341	ATTGAGGATGGTCCTTTTTGAAGCAGACAATTGAAACTCATTGCGAATATCTCTTGCCGTCTTACAGGAGTTGGAGGCAGCACGAATCACATTTCGTTCG	@?@DDDDABFHCFEFHIIII=HHCFGHGABBE3<DGHG>@FFH<FFDF=H>=CBCFEGGAAGIGHHEE>5@@B@C@CA?B=?AB;;?B<:@:A>3+8??#	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:1116:3353:52872	99	chrII	17407	60	99M1S	=	17466	159	TTACAGGAGTTGGAGGCAGCACGAATCACATTTCGTTCGTCACGCACGGAGAGAGCTTTGCGACGAGGAGCTCTTTTAGATGTACCGTAGCTCACCGGAT	CCCFFFFFHHHHHIJJIJJJJJJJJJJIJIJJJJJJJJJJJJJJJGIJJJHHHFFFEEDEED@BDDDBBDDDDDDCCDDDDDDDDDDDDBDDDDDDDBD#	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2211:10693:67001	99	chrII	17455	60	100M	=	17585	230	GAGAGAGCTTTGCGACGAGGAGCTCTTTTAGATGTACCGTAGCTCACCGGATCCTTCAGATACTCGCGAATACAGTGTCGAGAACGGGAAATTTTCCTAC	@@@DDDDFFHHGHJJJGIGJIGGIEGHIJIGGGDDBGHIDHIIGIIGCAEEHFFFFBCCECCCCDDDDDDDDDDCAADEBBBDDDDBDD<BCCDDDDCDA	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:1116:3353:52872	147	chrII	17466	60	100M	=	17407	-159	GCGACGAGGAGCTCTTTTAGATGTACCGTAGCTCACCGGATCCTTCAGATACACGCGAATACAGTGTCGAGAACGGGAAATTTTCCTACTCATTTCATGC	>DDBDDDDCCCCDCCA:CDDDDBBCDDEEDEEDFDHHHIIGGIJJJIHGFGIGJJJJJJIGJJJJJJJJIJJJJJJJJJJIJJJJIHHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1307:19886:52946	99	chrII	17486	60	96M4S	=	17520	134	ATGTACCGTAGCTCACCGGATCCTTCAGATACTCGCGAATACAGTGTCGAGAACGGGAAATTTTCCTACTCATTTCATGCAGGGACACATTGAGCAATTT	<@@FFDDDCCFGHIIIIGFH6EFGGCH>@H<D9B?F:FA4@FCH)).746=??B98/3953(;;>(5>:>:>@A>;5;>(:48&8@?BC>3>C>CC####	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:1307:19886:52946	147	chrII	17520	60	100M	=	17486	-134	GCGAATACAGTGTCGAGAACGGGAAATTTTCCTACTCATTTCATGCAGGGACACATTGAGCAATTTCATAACATCCAGCTGAGCGCGTTCAGTGTCCGAA	><CD>C@4:<BB>@C@<?D?A>CC;@9C;CB?DCDCEEAHEHHA@CCC88;GHCIIGGG<DHBHFBFDCGFGDJGJIHGBJJJIF@<8DAHHFFDDD@@?	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1105:2590:61441	99	chrII	17529	60	100M	=	17793	364	GTGTCGAGAACGGGAAATTTTCCTACTCATTTCATGCAGGGACACATTGAGCAATTTCATAACATCCAGCTGAGCGCGTTCAGTGTCCGAAAGGGCAGAT	CBCFFFFFHHHGHJIJJJJIJJJJIJJJIIIIIIJJJJJJJJJJJJJJJJHIJJJJJJJIJJJJJHHHFHFFFFFDDDBBDDDFDEEDDBDDDDDBBDB3	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:108:5608:15871/1	16	chrII	17573	60	101M	*	0	0	CATTGAGCAATTTCATAACATCCAGCTGAGCGCGTTCAGTGTCCGAAAGGGCAGATCCTCGAGGCATTGCAAGTTAGACTGCTTTCGAAGTAAGCTTTCCA	;5D???C=FDEEEAEB=?@??CC::@:CBBC?BBEBD5CCC:GAGFDGEGG?FGDEGAEGBGFGGEEEGGGGFDFGGGDFFGDGEGEGGGEGGEFFGBGDE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2211:10693:67001	147	chrII	17585	60	100M	=	17455	-230	TCATAACATCCAGCTGAGCGCGTTCAGTGTCCGAAAGGGCAGATCCTCGAGGCATTGCAAGTTAGACTGCTTTCGAAGTAAGCTTTCCAGCCTCTATATG	:>A>@4C@AC>CCAB>5B=3,(@>@@CDCFFGHHECIHGECCIDGF@:DEG@BFGHGDGAGGDFD4FBCEGHGB@EGGHGGEFGIGIHHHHHDAFFFC@@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1301:10889:93999	147	chrII	17605	60	18S82M	=	17346	-341	ATAACATCCAGCTGAGCGCGTTCAGTGCCCGAAAGGGCAGATCCTCGAGGCATTGCAAGTTAGACTGCTTTCGAAGTAAGCTTTCCAGCCTCTATATGTG	##################?CCC?A;9''<?>ECA?7=DC=)';7''**8/9<GD?09?*G@?9C9:*)FE?++HBBHC3@CGAHHEFA3:DFDDDBD?:;	PG:Z:SNAP	RG:Z:000	NM:i:1
+EAGLE:1:56:7956:12354/1	16	chrII	17709	60	101M	*	0	0	CCACATTTAATAATTCACGCAAAAAATAGTAAATAACATCTGTGAGGGACAATTTAACTTGAAATATTGGTCCCATGGAACCTTGTAATCAAAGAAAAACG	AEBEEEBBDEBDEBBFBFB=CEEFEEEEGGEDEDAABAB:AEEEEEDFADFFFBEDEGCEGFDFCFFAGDEGFGFGGGGDGDDFGFFDEFGGGGGGGEEGF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1202:3232:33845	99	chrII	17750	60	100M	=	17828	178	GTGAGGGACAATTTAACTTGAAATATTGGTCCCATGGAACCTTGTAATCAAAGAAAAACGATTTGATTCCTGATAAGCCTTCCATTGTTTCCTGCTGCAT	@B@FFFFFGGHHHJIJJJJIJJJGHGIIJHHIJIJJJJGJJJJIJJIIJJHGIJJJJIJJJJJJJJJJJIHHHHHHFCFFFFEEDEDDEDDDDDDDDDDD	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1105:2590:61441	147	chrII	17793	60	100M	=	17529	-364	GTAATCAAAGAAAAACGATTTGATTCCTGATAAGCCTTCCATTGTTTCCTGCTGCATATTTTGCCAAATCAGCTTGACTACACAGTCGAAACATCTAAAG	CCCDDDDDDCDDBAADEEFFFFFHHHHHHIIGFJJIIIGJIGGIIJJJJJJJJJJJJIJIJIJIGIJJIJJJJIIJDIIHIIJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1202:3232:33845	147	chrII	17828	60	100M	=	17750	-178	CTTCCATTGTTTCCTGCTGCATATTTTGCCAAATCAGCTTGACTACACAGTCGAAACATCTAAAGTGCGTGCTAGGAATTTTAGTAGGAACTTGTTAAAG	DDDDDDDCDDDDDEEEEEFFFFFFEHHHHHJJJJJJJJIIJIJIGIHJJIJJJJJIJJJJJJJIJJJJJJJIJJJJJJJJJJJJJJJHHFHHFFFFFCCB	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2316:7586:92994	99	chrII	18220	60	100M	=	18302	182	AGATAAACTTCTGGAAAAAAATGTATATAGGTTTTTTTCAATTTAAAAACGAACGGCATTAGCAGTTTTTCGAAAAAATTTTCGGAAGTTCCTCAAACCT	CCCFFFFFHHHHHJJJJJJJJIJIJIIJJIIGHIIIJJJJJJJJJJJJJJGFIHGFFCDDDDDDCCCDCDDDDDDDD@DDDDDDD<@BCDEDDDDDDDBA	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2316:7586:92994	147	chrII	18302	60	100M	=	18220	-182	CGGAAGTTCCTCAAACCTTCAAATTAGGGGGGGTCCTATAGAACTTTCCCACACTGTATATTGCAAATACATGACATAAATTTAGATGCAGGGCAAAAAC	DDDCDDDCCDCDDCCDDDEDEEDDDDDFIJJJJJIJJIJJIGDJIGIIHEFIFIJJIJJJJJJJJIIHCJJJJJJJJJJJJJIIGIIHHHHHFFFFFCBC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2112:17571:72038	99	chrII	19694	60	100M	=	19888	294	CCTCCATTTCCTACAGAACCTGCGGTTCAAACAATTAAACTTCCCAAATATATGGAGGTAACCATACACTTTTCATATATGTGTAACATGGGGCGGAAAT	C@@FFFFAHFDFFIIIIGHGGIIIJIGIIG<FGHIJGIJJIIJJIJIJGHIIIIIJJIJGIJGHGIGGEEFHHHDBDEEDDDCCCDCEDD?@B>@BDB9@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2112:17571:72038	147	chrII	19888	60	100M	=	19694	-294	CAAATTGTTATTTATTTCCGATTCATCTTTATAACTTCAATAATTTATTTGAGAATTCTATTCAATGCTATAAAAGTCAAAAATTTGCGAAACGTGGTTT	ADCCDCCDDECC>AACDBFEHEFGHCD:EGGDHHFA>@IHGIJJJGGGGGGHEHFAEHIIGGJIIFF@GGD@IIJIGGIIIGHGIJJHHHFDFDFFFCB@	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:2302:11666:55394	99	chrII	21802	60	100M	=	21978	276	CTTCTGAGAAACTCGAACTCATGATCAGAAACGCGCTCTGATCGAACATTCATACTCTAACACATTCCTCTTCACATCTCCAGATGATTATTCATGTCCA	CCCFFFFFHGHHHJIJJJJIJJJJJGGGIIIGGIJIJJJJJJGIGHIJJDIGIJJJJJGDEEHHHDEFDFFEECEDCEACCDCDDCCDCCCDDD;3;A;5	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:89:11611:13275/1	0	chrII	21896	60	101M	*	0	0	TGTCCATCATTTTATCATTAAATATCTCATTCTATACTCTCTTCTCGCTTTATTGTTTCTCGCTCACTCCCTACCCACCATTACATAACATCTCTGAAATT	EEEEEDEEDEEEEBEFFFBFEFFEEFFFEEGGGGGDGGEGGGDGGGGGFGGGGGGFGEGGGFEGGFGGGGGEEGGGEGE?ABBEEEEGEEEEEEEBEEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2302:11666:55394	147	chrII	21978	60	100M	=	21802	-276	ACATAACATCTCTGAAATTTCAAAGTTTTGACATTCTTGGCTGTGCCTTTTCCTCTCAATGATATTTTCCAAATTCCATTATTTTTCCCCCGCCTTTGAT	3CDDDEDCCA>3@DDDDDCCACDDDCCCDCDDDDDDDDDDDDBA??CCA<ABDBDDDEEEEEDCDCDCDDDCDCCCCCEEFFFFFHIHGHHGFFFFFC@C	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:109:12018:8736/1	0	chrII	22087	60	101M	*	0	0	TGGTTACTTGTTTATTGGTATACTTCTCGGTATTCTTTTTAATGTTCTGCATGTTTCATATGGTATAAATTGCATATTACGTTAGACACAAATTACTGCAA	:CC=ADDDDCFFFFFFFFFEFFFFFFFFFFDFFFFDFFEFF:FFFFDDFFEBEEFBBDEDEEB=DFFD5DBDEEEFFFAEEF?F:D:EBCFBBFFFDFFDF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:83:14451:5491/1	16	chrII	24168	60	101M	*	0	0	GAGATGCTGGAAGACCAGGACCAGTTGGAGAGCAAGGACCACAAGGACCAGAGGTATATTTATTTTTATTCGATATATTCAAGGCTTTACATTTATTAATT	FBBBAB=@DAEAB5EEEEFBF=EDEEFFEEEFFEBFBBCBBFFEFE@CEEE=FFFFFEFFFFEEFFEFEBFEFFFFFFFDFBFFFFDEC:EFDFFFBFFDF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2203:12843:33593	73	chrII	24481	60	56M44S	=	24481	0	GTACAGAGAGTGTAGATAGTTAGAGAGGGACATACATCCGGGACCCAGTGGGGCGGGGGGGGGGGGGGGGGGGCGGGGGGCGGGGGTGGAGGAGGGGGGG	@?@DFFDEDFADCGEGBEBAEHHCCEA+@@;?CFDD<?F:FB6@FH<D=F@=B8>@############################################	PG:Z:SNAP	RG:Z:000	NM:i:7
+BS-DSFCONTROL03:121:D0M8KACXX:5:2203:12843:33593	133	chrII	24481	0	*	=	24481	0	TCGAACTTTCTCGTGCAAAATCTCTGGCCTTTTCTGTATACTTTAGAATTTGAATTTTCATACGCATGGTTTTCTACACCAAATGAACACATCTGAGAAA	@DEDCDDDBBDDEEDFFFFFFHHHHJIIGG>JHIGGIIHGJJJJJJJJIIJJJJJJJIJIGCJIJJJJJIIIIHGFGCJJJJJIHEGHFHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2102:18771:39670	99	chrIII	1739	60	100M	=	1876	237	CACCACAATTCAATACTTTTCAACTCTTGAGAATCGAAATAGGGCAGCGTAGATATAATATCATTGGGTGACACAAAAATAATCGATAATTCCTTCACAT	CCCFFFFFHHHHGJJJJJJJJJGJJJJJJJJJJJJJIJJIJIIIJIJJJHFHIJJJJJJJJIIJJJJIEEHHHFFFFDDECEC@BDBDDDFACCDCCDDC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2102:18771:39670	147	chrIII	1876	60	100M	=	1739	-237	TGTGGTAACGTTAAGGTCTTGTTGAGTTTGTACAACAAATTCGAATTTCCTGACACGTCCCAGTAATATTCTCAAATCGTTGAAAGCTAGTTGCATGAAG	BDCCBDCCDDCDDDDDDEDDEFFDDFHHHHHHHJIJIIJIIIIJJJJIJJJIIIGJJIIJJJJJJJJJJJIHJIJJJJJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:51:14937:4579/1	16	chrIII	4052	60	101M	*	0	0	CTCAAAAAAAATTCCAACGAACAAAATGTGTAGAACTTTACAAACTTTTTAATAAAGATTTCCAAATTTTTATACAGATTCACTAAATTTCCAGAGTTTTC	EB7<<?BBAB=E?BEDBFC8DADBEDBEBBECD?@A@AC=CE=?ECDFFFAEEBEED?B5BB:EBEAFFAEE?BDECAC=CBADDEDEA?GGFGGGF:EFF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:59:9937:9794/1	0	chrIII	7949	60	64M1D37M	*	0	0	CATACAAATTCGAAGCGGTATTGTACGATCAAGCTGTTATTCACATTTTTTCATCCCAATTTTTCCAATTTTTGATGGAATTTTTGCTCAGCGAGCCTAAT	GGGEGEGGGFGGDDGGGGCGGGGBGGFBDGGGFGGBDDEFEEFEEFEFFGFEEEEGEEDEEEGFEEEEEGEEFE:CEEBCEEDEEBDDBCCDC?CEEEEE5	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+EAGLE:1:120:8762:5690/1	16	chrIII	9639	60	101M	*	0	0	CGCCGCGTAAACTTTGAAGCTTTTTATCTTTCTAGGAAGAAGAGTTATGAAAAAGTTGTCATCTAAGAAATTGTGTAGAATTTTCAGCTCTATAATATAAT	5D=AB=FFEDBFFDEFBEDCCCFEBFDDEEFFFFEFBF?FFFFFFEDAFDBDE?EBDFAEEFEFFFBDDBAFAFFFDDFFEED?EFBEEEA=EBEDDB=AD	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:1:44:18899:7734/1	16	chrIII	11923	60	101M	*	0	0	AACGAGTTTGAGTTGTAATAAATTATTAATTTTTTAAACTTTTTTTCCCAACATTTTTCAATTTTTTTGGGTCCCAGGGGGAAAATTGATGGGTATGCTGT	DAEE@EE@C@?GEEEGEAEEEEC=ECABC9EDDGDDCAA?CCCC::EAFECF:GGGGGGGCCFFF=B=AA?F?AFFGGGGFGGGEGGFDGGGGGFDGEGGD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2307:3105:84335	99	chrIII	15487	60	100M	=	15624	237	GGCTACACGTTTCTACATGTGATCATTCTATAAAGAATGGAGTGGTTGAAACTTTTGATTTTTTTGCTAAAAATTTGAATTTTTTTAATTGATTTTTTGT	CCCFFFFFHHHHHJJJJJJHHIIJIJJJJIJJJJJJJJJIJIGFHCGIGIGHIJJJJJJJJJJJJHHHHFFFFFFEEEEEEDEDDDDDDEDCDCDDDB<A	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2307:3105:84335	147	chrIII	15624	60	100M	=	15487	-237	TTTTATGCTAAAACTAAAATTTTCACGCCGAAACTCAAAAATTTCACATTTTCACCTTTTTTCTTGCCATTTCAACACTTTTTCCTGTAATTCTCTATGC	DDCCEDDDDDEDDCFDCA<DDB@DDBDFFEHEEHHJJJJJIJJJJIJJJIJIHDJJJIJJJJJJJIJIHIIJIIGECJJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:114:2367:17023/1	0	chrIII	15792	60	101M	*	0	0	TTTTTTTCCAACAGTTTCAGATTCATGATTCCGTCTACTTCAACAGATTTGCCAATTCCTGATCATATTACTGTGAGTTACAAAAAAATTTTGGAAAAAAT	GDGEDEGGGGGGDGGGGGGGFFAFFFFFFFFFFFFFFFFFFFFFF?FFF?EEEEDEFEFDEFEFFFBFFFFEDDE:FEFDFFFAFAC>CCFFBF=FFABEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2312:17962:78103	99	chrIII	19688	60	100M	=	19760	172	CTGTAGAGACCCAGGCAGGCGAAACGGGTTTGGCAGAAGCCGCTTCGTTACGCGACAGAACCATCCCCCACCCGCTTGTAAATATATACGCAATCAAGAT	@CCFFFFFFHHHHJJJJJJDGHIJIIIJ@GIGHHHICGC;FABEGEEHFFDDDDBDDDCCCDDDDABDDBBBDBB@@DBCDDDDEEEE@BBBBDDDDDAC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2312:17962:78103	147	chrIII	19760	60	100M	=	19688	-172	CGCTTGTAAATATATACGCAATCAAGATTACGCAACGCAGCTGAACGAAAAAAAAAAAGAGAATGGGGGAATCTTTGAGGGGAGATAATTGAGAGGAAGG	@DDDC@CEEDDCCDDBBCCDCCDCAADBBDBDBBBDDCDCADDDDCEDHIJJJJJJIJJIIIIHJJJJJJJJJJJJJIJJJJJJJJJHHHHHFFFFF@@?	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2109:9506:74934	99	chrIII	21761	60	100M	=	22497	836	CAAGGATCTATGCATGATTTGTGGTAGATGTGCCTGGAAAAGGGGGATTTTTGAGTTGTTTTCTAGGTGTGACCAGGTTTTTGATATGATTTTTGAGAAG	CC@FDDFFFHGGGGGGGIJIHFHIIE>B4<<?FGHIIDFIGEHGIJAEHBCC@AE@GHEHHFEEDFF);7;>AAC>@AC@BB?<C>>@>CDCCD>8<<><	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2109:9506:74934	147	chrIII	22497	60	100M	=	21761	-836	TCAACAAAAAAAAATCGAATTTGAAAACTTATAAAATTTATCCAATAAATGGGCGGAGCTTAGCCAGCTTTCTCAACAACTCACTTGCAAGGTAATAATC	CACB<@<B:CDDC@@B;DFD>;?EEA>EA@74JJIFCC;GHF:GIGIFBDGG@EGB<HEIHF?ECBHHB9E>DCA@>CAEHHGIJJJHHFFGFFFFD@<@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:41:8435:11353/1	0	chrIII	24117	60	101M	*	0	0	AAATTTTGGTGAAAGTGGGCGGAGCCTAAGTAAAGGATATATTTTTTAAAAATTATCAAAAAATGTTGCAAACTTTAAAAAAAATTTTAAGTTTTTTTTCA	GADGGFFFEEEEEEEEGGGGGGEGGGGFEG?BBCEFFFFFDGGFGEGGGCFGGFGGFGGEEEDEGEGGGFEEDEGGEEEGED@=6DD?EBGADBDDB=C?E	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:95:11679:7360/1	0	chrIV	621	60	101M	*	0	0	ATTTCCCCTCAATTTTCTTGCAGATCGACGTGGATATTGACGACCTAGATGACGATTTTCTCTCCACAAACGGAATGGAACGACACGGTTCAGAATTACAA	EF@@@EEEEEBBABBBECEEEEEBEBEFBDFDEAFEGD5DGGDEFGGGGDFGFG:DFFFFFGBGGGGGFGF?F:FEADBEEBEEEEBEBEEEE==:EAAEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:58:15136:13881/1	16	chrIV	723	60	101M	*	0	0	GAGAAGTGGTTTTCACTACGACGAGTACTGCGAATAACAATGTGAAACGAGCCGTCGAATATTTCGAAGATGATAATCAAGATGATGCACTAACATCCACT	@A9A9AAA??A?:?:??;@?:>>??=5A?A?@:C=CCADBCDDCFBAGEEEEFFGEFDEFFDG?GGFGGGFGDDGD?FFDGAEE=?EECDAFEEE?EEEFE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:25:10330:11791/1	0	chrIV	1025	60	101M	*	0	0	TTTATTGAGGAATACTCTATTGAGTTGGACACCTCTGGGAAGTATGTGTTGGGAAAAAAAATCCGAAAATTGATTGAAAAATTCGAAAAAAAATCCAAAAA	GGGGGGF=GGEGFFGGGFGGGGEGEFBFDFGGGDGGGFGFEFBFFEBEBEFFFEFFAFGGGEFGGEE=GDEDEBEGEDEDEFEEF@DEEEEG>CBBDDEBF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:29:6386:17433/1	16	chrIV	1252	60	101M	*	0	0	AATTGATGGATAAAAATTCCAAAAAAGTTCCAGAAACAACATTTCCTCCGCAGCTTCACCTGGTCCCAAGTCTCCATTTGACGATGATTTTACTGATACAG	E?===FBDGGFDEE=FE@GGFEEBFACAAD=FFFDEFEDGGFFEDFBAFGGFDFFF=BGGFFFDF=GDAGGG=GGEGFDGGGGEDGFFDFDEEEEDEEEDE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1214:19701:60229	99	chrIV	1474	60	100M	=	1594	220	GTCCGGTAGAGACCCATTTGATGAAGATGTACGTTTTTCGTAAAGTTCGGAATTTTTAGGGTATAAATTGAAAAACAAATGGAGAAAATATGAATACCTA	BBBFFFDDHHHHHJJJJJJJJBHIJJJJIGIIIFHIJIJJIJJIJIIJJJJIJJJJJJIJJDHHHHHHHFFFFFEACDDDDDDDDDDDDDEDDEEDDDDD	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1112:16947:43819	99	chrIV	1479	60	100M	=	2024	627	GTAGAGACCCATTTGATGAAGATGTACGTTTTTCGTAAAGTTCGGAATTTTTAGGGTATAAATTGAAAAACAAATGGAGAAAATATGAATACCTAGATTT	<@@DFFFDHHFHHIIIIIIIHI>FAFHIHGHEDHGCFHIG?F?DFHIIFIGHHGGG.=FEGFH>CDHGBHH2?@C??>>;AC@5@B3;5;>::>>>(:@@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1214:19701:60229	147	chrIV	1594	60	100M	=	1474	-220	CCCAACCCTAATATCCACAATTTTTTTTCAGTCTGGAAACTCTAACGAAAACCAGCTCCGCGAGAAGAAGCTCCACAAAAAAGAGCAACTAGCTCACCGG	BDBBCDCCDCCCDCBC@DDDDDDDDDDDDDDDDEDDCA;DDDDDBDFFB?=HHHIJIJJJIJJJJJJJJJJJJHFJJJJIJJJJJJHFHHHHFFDDFCCB	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1112:16947:43819	147	chrIV	2024	60	18S82M	=	1479	-627	ATTAGAAGAGAAGAGGAGGAAGAAGTGGTGGAAGAAGATCCAGCAGTGGTAGTTCCAGTTCTACGGAAGGATCTGGAAGTTGAGGAGGCTCCGAAATCGG	##################CAA>;A;(>D?;>?HEA=74E:C=>@@;=8=89FBFDCGFD;69D:?AD?9?1*9<:9E??AA+;FGFGD<:8A;BA=+1=:	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2204:2799:95211	99	chrIV	2692	60	100M	=	2824	232	TTTCAGACGTCTGTATCCGCCGATCCAAAATCTCACACGCCGATTTTGATGGCCGACGAGTACAAAGAACCACAGAAGCAAGGTGAGTTTAGTTCGAAAA	CCCFFFFFHGHHHJJJJJJJJJJJJJJJJJJJJJJJJJIIJJJJJHHHHHFFFFDDDDDD8ACDDDDDDDDBDDDDDDDDACB:CCD>@CEDDDDDBDDB	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2204:2799:95211	147	chrIV	2824	60	100M	=	2692	-232	CATTGTTAGAAAATTTGAAAAAAAAACCAAAAACCGTCGTAAAACTACACTTTTTCTATTGAAAATAGAACAAACAAATCAAATATCCTACATGTAAAAT	:@4(DDDDDDDDDDCDDDDDDDDECDFFHHHHBJJJJJJJJJIGIIGHGJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJHJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2203:18040:19562	99	chrIV	2921	60	100M	=	3122	301	AATTTAAATCGAGTGGAATTAAATTCTTCAAACAAAAAGAAATCTGCATTTAATATCGATTTCTCATTCAATTGTTATGAATCATAAAACATGATTTTTT	CCCFFFFFHHHHHEHIJHIJJJJJJIJJJEHJJJJIIJJIJJJJJJJJJIJJIJJJJJGIJJJJJIJJJJJIIIHDHHHHHHHFFFFFFEDEEEEFEDD@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2203:18040:19562	147	chrIV	3122	60	100M	=	2921	-301	ACAATTGGGATTAATTTTCAACTGAACTAGACTGTTTCTGAACGCTCAAAACCTTCAAAATCAATGCTTCTTCAAAACGAAAAAATTGCACACTATAAAC	CDDCCCCCDEDDDDDDCCECACEEEFFFFFFFGHHHDJJJIIIIJIHIHFHJIJIIJJJJIJJJJIJJJJIHIJJJIJJJJJJJJJIHHDHHFFFFFCB@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:51:5632:6609/1	16	chrIV	3456	60	101M	*	0	0	GATACTGCATTTTTCCGGAAAAGGACAAAGTCGGCTAATTGCCGTTTTTTATAATTTTTGCTCATTAAGCTTATTCCATTAAAAATACAATTCTTGTTTAA	ACA;C@CDBCCEEEEEEEEEEEEEEEEEBEEGEEFGEBGEEGGEEEBEEDGEGFEFGGGGGFEGGGGFGGGGGGGGGGFGGEGGGGEGBEDGGEFGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:41:11958:20308/1	0	chrIV	3987	60	83M1I2M1I8M2I4M	*	0	0	CTTTGAAAAATAGGGAATTAAATTTAAATAAAATTTTCAACTGAACTAGACTGTTTCTGAACGCTCAAAACCTTCAAAATCAAATGAAATATTCAGGAAAA	GGGGEGGGGG?CCFFGGGGFEGGGGGFDGGGFEGGGGGFGGGGDGFFGGGGEGEE?FDFCGEEEEGGEEEEGEEEEEEE@CDDCCEBDDBEAEEEDBEEEA	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:2216:15913:7063	99	chrIV	4720	60	100M	=	4891	271	TTCAAGAGCCTAAGTTTTTTTTTTCGAAATGAGTTTTTTAAAATTGATCGAAATGTTTTTAAATACTGAATATTTGGGATTCTAGATTTTTACTACAATA	CCCFFFFFGHHHFIHJJJIJJJJJIJJJJIIJIFHIJJJHHHHHHFFFFFDEDDDFEEDDDDCDEDDEDDDEEEEDDDDDDDDEDDDEDDDDDDEDDDD>	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2216:15913:7063	147	chrIV	4891	60	100M	=	4720	-271	TCTAAAAAATTTTTTTAGTGCCTGCACCAGTTGTCGTTGCTCAGGAGTCGCCGATCTTAAAACGAAGAAACTCTCTAGTACCATCAAGGATCAGTGGAAG	CCDDCDDDDDBDDCDDDDDDDDDDDDDDDDDDDFFFFHHHHHJJJJJIHFJIGIJJIIJJIHIJJJJIGHJHHDJIJIHHJJIIJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:49:18520:10880/1	0	chrIV	6174	60	101M	*	0	0	GATTTTGGATTAAAAAATTCCGAATAAAAATCAACTTTAATTTAACGAAATTAATTTTCTATACCTAATTCTCTAATTTTTGCAGAACAGACGTAACGATG	EEEBEEDAA?DDB?ADEEEEEEEEEEEBEE>EEEDE?EB=EEEDBEE=EEEEDE5?ADBDEEEEEEEAEEEAEBE:DDBCDEE?EBDEDEEEEB:EEEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1103:16672:84478	73	chrIV	6412	60	56M44S	=	6412	0	TTTCGATATTCAACACTACCTGCCCAAGAAGTATAGCTCATTTGAAGATCAGTCACGGCTGAAGAACATTCTCGCCGAGCTGCACGGGCATTACGCGGGC	1==A1AA+<4,2>A3A3A7A@=?AABC?9AA:=<*7?*=*:7*0***9?79<A?AB############################################	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1103:16672:84478	133	chrIV	6412	0	*	=	6412	0	AAACGGAAATGCTCCGGAACCTGATGTTGGCCTCGTTGCGGACACACGATCCACATGCGCTCCCAAATCTCGGTGTCGCTGACAGATTTGAATGCATTTG	+:8;?2=:CDB4B4+@####################################################################################	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2305:20092:23229	99	chrIV	7114	60	80M20S	=	7551	528	GCCGTATCCTCTTTATTTGAAGTGCATGTAAATTTAAAAGAAGATACTTTTCGCTTTTTCGAGTGACCTTTTTTCAGAGAAAATATGTGCTTTTCTTGAA	=??DDDBD?DDDD?,C?BFDC@,<EAFE+AEF<FFD9B4?CDDEAF<9*:9B?0?8?D>=ADA8677)7A7AED7;3??@####################	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2305:20092:23229	147	chrIV	7551	60	9S91M	=	7114	-528	CAGTACTCCTGTATTAATCCTACAGTAATCCTAGAGTGCCCCTACAGTACCCCAATAGTATTTCTACTGTACTCATTTACTCCTACACCTAGAGTACCCT	#########C>>3@>>;)@@BDCA=)>?77@@CGEG@;F;94?/AD@?0BBDHHFHFB9BBB@FDF<CCHAE?GCHA?AC+HEBDGAHHBF??D?AD@?B	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:25:14196:18951/1	16	chrIV	8235	60	101M	*	0	0	TTGTTTTAATTAATAAAATACGTACACTAATTCAAAATATATGTTTTATCGATTTTAAAACCTCAAAAAAATTGTAAAAAAATTGAAAATTAACTGAAAAT	CEEEEDAFD=CEEEBEBEEBEGFFGFEDGGGAGEEGGDGEGFGFEDGEDDEGFEEGGGGFGGGGGGGFGGFFFFCFFDFDFEEEEEE:FFBFFF:FEGGF?	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:115:8880:13347/1	0	chrIV	8542	60	101M	*	0	0	GAAAGCGGTAAGAATTCGAAATTTCAGTCAATATCCCTTTTCCTCGGGGTGTACACTAGAGTTGTGTAAATCTACACATAACGTTTTTTCGATTATCTCTG	GDGGFGEDBGFFDFFEGGGGEFGGGGGEEG:EEBEGGF?DEGFBDEAAE<A?CCBEEDEBE?AB=:CCCCCF?EBFD:DFBDDCCCBCACE@EEECEA:55	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:25:8054:8779/1	16	chrIV	8985	60	101M	*	0	0	GGATAGATTTCCATGTGGGCCAGGCATTTTGCTAGCTGAAATTTTGGGATTTTTACTTACAACTGGTTTAGAATAATTTAGAAATTACAAAATCGAGGTTT	5B5EBEBB?C=E5:=EEFEFDEBAEE?:D=ECE::DFFAFFFD:=FEBDDE?B:ADBBBAECEBDBDBCFBEEDDADED=?ED5?DE=:DEFDDDD?EEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2213:7767:73428	99	chrIV	9395	60	100M	=	9538	243	ATTGTAGGCCTTGATAAGATATATGATGACCCGTCGTTGAAACCACGTGGCTGGAGATTAATTCTATCAGTTAGTCAAAATTTACTGACAATCACTCAGA	CCCFFFFFHGHHHIIJJJIJIIJJJJIJIJJJJIIIJIIIJHIJJIGIGGIGFHGHIJJJJIJHHHHHHHFFFFEEEEECE@CC@@CCCDDDCDDCCDD:	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:2108:20580:46115	99	chrIV	9443	60	100M	=	9624	281	GGCTGGAAATTAATTCTATCAGTTAGTCAAAATTTACTGACAATCACTCAGACGCTCAATATTGCGATTTTGCTTCAAAACTACTGTACCCGGTCTTGAC	@@;DDA+B<FDHFC?EHGGGCIHICF3AFHFCFFCGEGGCFHIHDDD>FHGIHGIII6B8=B8@=<;=DHHECCEACCB@@@:>CCCCC@CBBBBBC<5@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2213:7767:73428	147	chrIV	9538	60	100M	=	9395	-243	TTGACATTTTTTTTGGGAAACATGAAAAAAATGAGCATGATTTTTTTTCTATTTTCGATAGACCTGAATATATTTCCGAAAAATTCAACCCATCCATCGC	EEDCCDDDDDDDDDDCCEEEDEDDBDDEFEDDDDDDDEEDDHJJJIIJJJIGFIJJIIGGHFJJIJJJIIGIIJJJJIIJJJJJJIHFHHHHFFFDDCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2108:20580:46115	147	chrIV	9624	60	100M	=	9443	-281	AACCCATCCATCGCTCACTTACTGTGAAAAAGTTCCACTGCAGCCCGAGATAGTGATGTTTTGTGGGCGAATAGTTGTCGAGAAGCGTTCGCATGTTATG	89<2AC?5BBB>A>@C@DDD@@:CCCCC@A@>A;>>;;?1DCBB?=;CC=4C7.)ADF@BDB;0>F?F?B@HDAHBD?CEHGGGFC@87HHD?ADFD@@@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:45:8531:9371/1	16	chrIV	10605	60	101M	*	0	0	TTTAAATTCAAAAATTTACATAATTTTTTAGTTTCTAGTGCCCTACGTTATTTCTCCTTCCGATAGAAATAACACTAAATCGACAAAACATTTTGCTCAGA	:CAEFEDCEGFEDDEEDEEDEGEFEG?FEFDEF?FDCGEGFFGEFGGGBGEG?GGGGFDGGGFFGGEGGGEGFFGGGGEFGGGFGFGGGGGDGFGGGGFGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:45:5297:17573/1	16	chrIV	10799	60	101M	*	0	0	AGAAACAACACAAAAACCGGTAATCATTATGGAAGTTCGTTGTGTCAAATTTGCACGATTAGTGAAAACGCTGATCTTCGATGCTTAAAGACTTCATCGGA	EBBECBBDC@EDFDCCFBAEEEEBGFGGGGEEFFGFEAFDBGEFGGAGGGEGFGDGFGGBGDBGGEEGGGFGGGDDFBGFFEGFGGFFFFBFFA?F?GFGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:90:8728:2691/1	0	chrIV	11034	60	101M	*	0	0	AGATCTGTGATAGAAAGACGAGGAGGAGACTGATCATTTTTTGGAAAAAATAGATTTAGTTAATTAATTAATTAATTTAAAAATAAGTATAAAAGTCGTCG	GGGGGFGGGGGGGFDGGGGGEFFGGFEFGGGGFGGG:AGFGGEGEGFFGGEGEDGGBFGEFGEGGGGEGEDEEGEEEGBEEEEEEACAEDDDBDEADB?BD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:58:9805:10604/1	0	chrIV	11061	60	101M	*	0	0	GACTGATCATTTTTTGGAAAAAATAGATTTAGTTAATTAATTAATTAATTTAAAAATAAGTATAAAAGTCGTCGTCTCTTCTGCCTCTTTTCGATTTTAAA	GGGGGGGGGGGGGGGGGGGGGGGGFGFGGGGGGEGGGGGEDGGFFGGFGGGGGGGGGGGGEGGGGFFFCFFDFFCGDGEDGFEGGFGEEEDGE=DEDDDCE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2102:2939:36410	99	chrIV	12069	60	100M	=	12243	274	GAATTGATTAGTCAAAACCTTACAACTTGTGACTCAGAATTTTGAAAGTCTACCGAAAAAATAACGTTCTTCAACAATTACGATTCTTCAATTCTAATAG	CCCFFFFFHHHHHJJJJJJJJJJJJIJJIEHGIGGGGIIJJJJIIJJGGIGHIIIJJIJGH2BEGH?EEFF>DEECDEDEC@B?ACCDCD:;ACDACCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:23:5518:6512/1	16	chrIV	12134	60	101M	*	0	0	GTTCTTCAACAATTACGATTCTTCAATTCTAATAGGTTTTTTTTCCGAAACTTTGAGTTTTGCTACCTCTAAAATATGTGATTACAAATTATGGAAACGTA	?CBAC=CA@:AEBBBD=FCEEE?=EECBA?CBFDFC>:EE=:C:EEEEBEE5FAEF=EEEEDEEBEFBFFDFFFFFFBFEEFFBDFFADFF?DDDD5ACC=	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:9:17295:4977/1	0	chrIV	12165	60	101M	*	0	0	ATAGGTTTTTTTTCCGAAACTTTGAGTTTTGCTACCTCTAAAATATGTGATTACAAATTATGGAAACGTATTAACAAATTACAACAGTACACTTGGTGTCA	AEECDEEEEEDGGGGGFGGGFGGGFGGGFFDEEEEGGGGGGFG?GGAFGGGFGGGFFGGFGGG=GEEEE?E=EEEFFGDDGG?AGABAD?=CEBEEADBEB	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2102:2939:36410	147	chrIV	12243	60	100M	=	12069	-274	TTACAACAGTACACTTGGTGTCAGAGTGTCTTATTTCGGCTACAAAAAATGCGGGTAAAGAAACGCAGAGTTCTCAATTAATTTCGCATGGTTAAGAACG	ACCCDDCDDDAC>DDDDDDDDDDCCCDDDDDCDBBAFFEFFHHHJJHJIIIJIJJJJIIIHFDHJJJJJJJIJIIJJIJJJIJJJJJHHHHHFFFFF@CC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1211:1649:59300	99	chrIV	12490	60	100M	=	12628	238	CTGCCGGCCGTAAAAAGTCATTCACCTCATCATCCGGCGATCTTCTCAATGCTCCAAACCGCACTAATGGACAATCCCGAACACCACCTGCTCCACGAAA	CCCDFFFFHHHHHJJJJHIIIJIJJJJJJJJJJIJJJJIIHHHHHHFFFFFFEEEEEEDDDDDDDDDDDDDDDDDCCDDDDDDDDDD@BCDDDD@CB9@@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1211:1649:59300	147	chrIV	12628	60	100M	=	12490	-238	GAGCAATCGGAGATGCTCTGCCGACTCTTGACGGCGATAATCTCATCAATATCAAGGCGATCTCGGGGCTTCATTTGGATGAATCTGATGAGGAAAAGGG	DDDDDDDDDDDDDCAB?DBDDBC@BDDDDDFFHHIGJJJIJJJJJJJJJIIGJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:57:18691:3342/1	16	chrIV	13429	60	101M	*	0	0	ATTCAATTTTCGGCAAAAAATAAATTTATGATGAACAAGTTGAGATTAAAATATAACTTTTCCTCTGATGAAATTTTTTTATTTTATCAAAAAATCGGAAA	BBB=FDFFF?FAE;C?EGGGGGGFDGDGGDFFEGBGDEGGGGGF=GEGGFGFGGGDGGGEGDEGDGEEECDDDGGGCCBEAEFFDDDDDE=FFAFFGGFGF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2304:10466:54597	99	chrIV	13926	60	95M5S	=	14386	560	GTTCAAAGGCCCCCTCCGGCATACTCGGATCAGCATCATGCGAGTTCGACATCTGCTTCGGCTCCGCTGATATCGGTTATGCTTCTGAAGAAGCCGATGG	C@@FFFFFGHGHHJJJIJJJJFAFGGGIGIIIJIJJEIJJJJFIADHGEHBDFEFFFEDDDDBDDDDDBDCCACDD?3<CCD@AACACDDDDCCB#####	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1103:15940:55697	99	chrIV	14066	60	100M	=	14107	141	GGGCTGTGCGGCTACCGAGTTGCCAAAAGTTGGGGTTCGGCAACTTCGGCAATTGCCGGTTGCCGAAAATTTTGGAGTTCGGCAACTTAGGCAACTGCCG	CCCFFFEFHHHHHJJJJJJIJJJJJJJJJGIIJJJGIJJJHJJHHHHHFFDDEDDDDDD=BDDDDDDDDDDDDCDCC>CDDDDDDDCD@CDDDDDDDDDB	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1103:15940:55697	147	chrIV	14107	60	100M	=	14066	-141	AACTTCGGCAATTGCCGGTTGCCGAAAATTTTGGAGTTCGGCAACTTAGGCAACTGCCGATATCAATGTCAAACATTTTGTAACCCATAAAAAAACATTC	@<BBBDDDCDDBBDDDDABBD@?CDDDDDDDDEEDDFFGHGHJIIJJIHFJIGJJJIIJJJIJJJJJJIIJIIJJJJJIJJIHJJJJHHHHHFFDFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2304:10466:54597	147	chrIV	14386	60	100M	=	13926	-560	GGTCGTCTAGCCTATCTGAGATTGCCTACGCTCACCTTCATGTTGGCCGAGCCTAAACTCTTACCTACGCCTACTTTGTACCTACTGAGCAATCTAGCCT	BBB<>><>(@>@C>3>ACDCA=;5BB=9=3?7;7CHHHHG?GIHGGGHDBJJJIJIGIHGEB?JHF?IIHHCFJJIGHHEJHEIJIGHGHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:45:7639:19200/1	16	chrIV	15054	60	101M	*	0	0	CGTGTCAATACCGTTGCCTTATTCTCTTCAAATTTCTAATTTGTAACAATTTTCACACACACACTTATACAAAAATTACGGAAAACTCTCTTGAAACAACG	C?BBBBBA@>EFEBDDBB=EABBFAED:FF=EEBBBAE;BE=E?EAEBDBBEBDBEBEBEBEAEBBFC=FFFFECDFDFFFBGGEFFGFGGFFFFEEECEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:32:13629:16208/1	16	chrIV	15294	60	101M	*	0	0	ATACAATATTACTGTGATAGAAGCACTGAAAATTCTCTTTCATAAAAAAATTATGTAATGCGTTGTTGTTTGCGACAGACTTTTATTTATATATGACATTA	DBDGGBGEFFEFFGGEDGAGGFGGEGAFAF?DGDBGGFGDGFGGGDGFG=GDGFGGEGEGGFGGGGGGFGFGGGGGGGEFGDGGGGGGGGGGGGGGGGGGF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1210:18903:53675	99	chrIV	16174	60	100M	=	16449	375	AGTATAGGTTGAGTGTCATATTCGAAAGTGGCCTCCACTCCTCCAAATATTAGGTTATGGATTTATCTGTGCTGTTTCACAAGAAATGCCCAAGCTTCTT	@@?DBDDFFHHDDHIBGIIEHJJJEGIG<FHBEHIJIIGGIBFEGGGIJIJIIGFFGIJIIIEHGIIGCFHGDHIGIHHGHFHFFFFFFEEC=ABDDDCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:86:18150:16897/1	0	chrIV	16311	60	101M	*	0	0	TAACTCTAGAGCCGTTTGACTGAATTTTATAATATTTGACAGCTTACTAGAAATATTTCACCCCACGGGGGAGCAAAAAACTGCACAAAGTTTGGAGCAAA	=EFGGDGBGDGDGGGGGGDGFEF=EFFFAFAFEFDFFEFBF?FBFFFFFDFFFDFFFFFFFFFFEF>FBFB=>9B5??>=F@DFDEB=?B?B@?B@:@@=B	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+BS-DSFCONTROL03:121:D0M8KACXX:5:1201:4433:4114	73	chrIV	16371	60	100M	=	16371	0	CCCCACGGGGGAGCAAAAAACTGCACAAAGTTTGGAGCAAAACTGAGCTCAGGACGGCTTTCCCCAGTTTGAAAAAAAATTTTGTTGCTCTTTTTACCCC	@@@FFDFD8CFFHGGGIIJB@HGJFIIJFE.@?ECHGFFD@CEEDECDC;5?CABB@B;5?>@ACC@ACC@:ACCDD9BB@>AAA98:>CC@CCC?>>38	PG:Z:SNAP	RG:Z:000	NM:i:3
+BS-DSFCONTROL03:121:D0M8KACXX:5:1201:4433:4114	133	chrIV	16371	0	*	=	16371	0	AAAATTAGTTTTTCGTCTTTATCTCAAGTTCTTCTTCATCTTTTTCGATACTTTTTTTTTCTACCCCACGTAAAAAAGTACGCTGAACACGATTTTCAAC	:@:4(:4<@9<B<3CCC@:>@8>::>:?>>99(@>>>:?@?BBCB?;>5(BCEFIGHGB=67(HED;?BBEIIGDEF@<3<@<?9GF?AA=<DDDDD@?@	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:1302:19827:42088	73	chrIV	16422	60	100M	=	16422	0	GGGCGGCTTTCCCCAGTTTGAAAAAATTTTTTGTTGCTCTTTTTACCCCCAAAAAACCATGTTTTAATTGAATTAAAATTCGGGTTTTGCTCGGTTTTGT	BCCFFFFFHHHHGJGHGHGEHIJJJJGGJJJJJIJJIJHHHFHHFFFFFDC@DDDDDDDCD>CDCDDCDCDDDECDDDDDCBBDABDDDCACBD?>@DBA	PG:Z:SNAP	RG:Z:000	NM:i:2
+BS-DSFCONTROL03:121:D0M8KACXX:5:1302:19827:42088	133	chrIV	16422	0	*	=	16422	0	GCCTTTTGTTTTATCACAACTTTTTTCCATTTGCACTTATGAACTCAATCTTTCTTTCAAAAAATCAGTCTCTCTGAGTAGTATCTTGCACAGAAATTTG	CCCFFFFFFFHHGIIIJIJJJJJJJIJJEIIJGIGHGHIJJJJJIJIJJJJJJIGIGIJIEHIIHIIGGHHHHGHEFFBBDBDEEEEEDDDD?CDACCDD	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:1216:8211:57172	165	chrIV	16449	0	*	=	16449	0	ACATGAAATAATGCGAAATGGATTTTCTAATTTGTAGAAATTATAAGATAGCTCCGAATTCTGCCAAGCAGCCTCTAATAAATGCCGAGTGTACAGACTC	?BBFA=ADHHGHHJJJJJJIJIJJJIHIJIIJJJIIJJJJJJJJIJIJJJJIJJIJJJJJJIJJIJIJEIIHHHHFFFFFFFEEDDDDDBDCDEEECBDC	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:1210:18903:53675	147	chrIV	16449	60	100M	=	16174	-375	TTTTTGTTGCTCTTTTTATCCCCAAAAAACCATGTTTTAATTGAATTAAAATTCGGGTTTTGCTCGGTTTTGTTCCAAATTTATGTGCAAGATACTACTC	8DDBBDDCC<?5>A:><:@BBB@>;;@>?>FHFFHGHIIHGHBGGGIIGFHGHGGB;GEH@0EGE7IGGHGCEF@BHJI<HGBGA9HBFFFC=:BDD@??	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1216:8211:57172	89	chrIV	16449	60	100M	=	16449	0	TTTTTGTTGCTCTTTTTACCCCCAAAAAAGCATGTTTTAATTGAATTAAAATTCGGGTTTTGCTCGGTTTTGTTCCAAATTTTTGTGCAAGGTACTACTC	8BBB?DDCDDBBCDDDDBDDDDDDEEEEEFFFDFHGHHFHHJJJJIJIIHIJJIIJJJJJJIIIJJJJJJJIJJJJJJJIJJJJIJJGGHHHFDFDFCB@	PG:Z:SNAP	RG:Z:000	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2211:12400:9160	99	chrIV	16457	60	100M	=	16639	282	GCTCTTTTTACCCCCAAAAAAGCATGTTTTAATTGAATTAAAATTCGGGTTTTGCTCGGTTTTGTTCCAAATTTATGTGCAAGATACTACTCAGAGAGGT	CCCFFFFFHHGHHJJJJJIJJJIJJJIJJJJJIJJIJJJJJJIJJJJJJGCGIJJJJJHHGFFFFEEEEEEDDDDEDFEDEDDDDDCC@CDDDDCCDDD3	PG:Z:SNAP	RG:Z:000	NM:i:2
+EAGLE:1:46:17265:14051/1	16	chrIV	16476	60	62M3D39M	*	0	0	AACCATGTTTTAATTGAATTAAAGTTCGGGTTTTGCTCGGTTTTGTTCCAAATTTATGTGCATACTACTCAGAGAGGTGGATTTTTTGAAAGAAAGTTTGA	D<>A7?@C==EADFFGFFBE?B?D@=CFFFEFAGGGGFGGGEDEBBBA?BFFFFEDC?DDB?C>C?CA=:=EDDBDGFFGGGGFDGAEEFDF5FFFGDGFB	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:1:94:17078:10619/1	0	chrIV	16610	60	33M1D38M1D30M	*	0	0	ATAAAACAAAAGGCCAAAAAATGACATTTTGCCAAAAAAATTTTTTTTCCGAAAAAGTAGTTTTTCGTCATATCTCAAGTTCTACTTCATCTTTTTAGATA	GEGGFGGGDGFFGGGGGGGFGGGFGBGDEDGGGGGGGGGGGGGGGGEEGDGGGFEEFCDECADEBFG@EDAEEBEEC?ABDEDDFEEEEEA?DDBC?BDC@	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:2211:12400:9160	147	chrIV	16639	60	1M1I18M1D80M	=	16457	-282	TTGCCAAAAAAAAATTTTTTCCGAAAAAGTAGTTTTTCGTCTTTATCTCAAGTTCTACTTCATCTTTTTTCATACTTTTTTTGTTTACCCCACGTAAAAA	DCDBDDDDDDDDDDDDDDDDDDDDCEDDDDDDDDDDDDEEFFFFFFHHHHHHIIEECIIJJJJJJJIJJHHHFDGJJJJJJJJJIHEHHFFHFFFFFCCB	PG:Z:SNAP	RG:Z:000	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2201:10133:75100	165	chrIV	16645	0	*	=	16645	0	AATTCACACGGTTCAGGAGTTGGAAATAAAAACGTGGTGTGACATTTTTTCGCAAGCCAAAAAAAACGCGAAAAAACGCGAAAAAGGGGCGGAGTCTGTA	#####################################################B@A;/DDFHIGG@GIEIIJIIHFIIJJIJJJJJJHHGHHFFFFFCB@	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2201:10133:75100	89	chrIV	16645	60	76M1D24M	=	16645	0	AAAAAAATTTTTTTCCGAAAAAGTAGTTTTTCGTCTTTATCTCAAGTTCTACTTCATCTTTTTCGATACTTTTTTTTCTACCCCACGTAAAAAAGTACGC	>BCDCA8@@BCB?<50+CDDCC@C@BDDDB@@CDCDCADBCCDDDDEC@;9EEEDFFFHHIIHHEEF=GJJJJJGGGGD?IIGHDIGGHHHHFFDDD@C@	PG:Z:SNAP	RG:Z:000	NM:i:5
+EAGLE:2:31:3792:4681/1	0	chrIV	16705	60	101M	*	0	0	TTTTGAGATTTTTTTTGTTTACCCTACGTAAAAAAGTACGCTGAACACGATTTTCAACTCAGAATTGAGAAAAGTTCTATGAGTCGGCCGAGCAAGACGAA	FBFFF?GGGGDGEGFEGGGEGGGGDGGGGGGFFGGDEGGGDFGFGGFGGGDDFDGGGGGGEEDEGGGGGFGGDEBDFEFFGF?DGEFGGGEGBBEFEEEAE	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:48:7607:3697/1	0	chrIV	16768	60	101M	*	0	0	ATTGAGAAAAGTTCTATGAGTCGGCCGAGCAAGACGAATAAGTGCCAAATTTTGCCCACTTTCCCGTTTTCGCGAATCTACTTTTTCCATCATAACTCGGT	GGGDDGFFGGGGFGGGFDEGEFDFFFF?DFFEFEDFFFFDBDADCFFFFFFG?GGGGFEFGGGGGFBD:BEEEBA@EEBED@?DDDDEDADBD:DB=ABB8	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+BS-DSFCONTROL03:121:D0M8KACXX:5:2304:5957:30377	73	chrIV	16778	60	100M	=	16778	0	GTTTTATGAGTCGGCCGAGCAAGACGAATAAGTGCCACATTTTGCACACTTTTCTATTTTCGTGAATCTACTTTTTCAATCATAACTCGGTCAGTTTTCA	BCCFFFFAHFHHHIFIIIJJJIIIJJIJIJJG>FFHIIJIIIIIIGIJIJGCGIHEHHHHHFFFEAECEACCCDDDDCCDDDD@CCDDBB@B@C:>@CCD	PG:Z:SNAP	RG:Z:000	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2304:5957:30377	133	chrIV	16778	0	*	=	16778	0	ATGAAGTTTCGTCGCATTAGAAATACTCTGCCAAGAAATATTATTTTTATTAATTTTCAGGCCAAAACTTGTTTTTTTCTACAAATCGACCGGTTTACGA	CCA@>DDDDDB?BACCDDDCDDC@@DDDDDDDEEEEFEEDFFFFEHHHHHGHJJJJJJIHFGGJJIGJJJIJJJJJJJJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000
+EAGLE:2:84:15670:14903/1	16	chrIV	16791	60	101M	*	0	0	GCCGAGCAAGACGAATCAGTGCCGAATTTTGCACACTTCCCCTATTTCGCGAATCTACTTTTTCAATCATAACTCGGTCAGTTTTCAATTTTTCTTAGTTT	BCADAEB?CCBDFDCDBEEBGEGEEEEECECFCD@FEDFGGEFFEDDFFFFBGEGGDFFFFFGGGGGFGGGFGFGGGGGGGGGGGGGGGGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+BS-DSFCONTROL03:121:D0M8KACXX:5:1106:14433:6441	165	chrIV	16828	0	*	=	16828	0	ACCGTCGGCTGAATTTATAAAAATAGACCATGAGAGGAATTTTTTAAGTCAAACCAAAAAAAACGCGAAAAAACGCGAAAAAGGGGCGGAGTCACCACAC	######################################################################GB:0))*?C9;GE@+C<?HD?=++=DD;=8	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:1106:14433:6441	89	chrIV	16828	60	5S95M	=	16828	0	ACACTTTCTCATCTTTGCGAATCTTCTTTTTCAATCATAACTCGGTCAGTTTTCAAGTTTTCTTAGTTTTCTAAAAATTGACGTGTAGGTCTTATCAAGA	#####CCCCCCC>?=385;(@>6.(46;@C=:ACAEHHE;@6FCEDBIGCHD<GFD:9HCFF@HGIHFFBF=IHHFFCAACFBEA@BAFHDHD;A1D=:8	PG:Z:SNAP	RG:Z:000	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:1306:16001:53612	99	chrIV	16842	60	100M	=	16921	179	AATCTACTTTTTCAATCATAACTCGGTCAGTTTTCAAGTTTTCTTAGTTTTCCAAAAATTGACGTGTAGGTCTCATCAAGACGCATCGAGACATATAAAA	CCCFFFFFHHHHHIJJJJJJJJJJJJFHJJIJJJJGIJGIJJJJJIJHIJJJJJJJJJJJJJJJHIJJIJJJIJJHFHHHHFFFDDDDDDBDCDDEECDC	PG:Z:SNAP	RG:Z:000	NM:i:4
+EAGLE:2:76:14252:18422/1	0	chrIV	16876	60	101M	*	0	0	CAAGTTTTCTTAGTTTTCCAAAAATTGACGTGTAGGTCTCATCAAGACGCATCGAGACATATAAAATTTGTAAAAAGTTCAGTGGGAAAATTTTTCAAGAA	DDDADBFFFFGGEGBGGGGFDDGBGGGFGDCFFDFFDFFFAEEEEGGGEGGGGDGGDGGGGGDGGEDGEGGGDFGGGBDGFFBFFGEGGBDBBFFG=DG?F	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+BS-DSFCONTROL03:121:D0M8KACXX:5:1306:16001:53612	147	chrIV	16921	60	100M	=	16842	-179	GACGCATCGAGACATATAAAATTTGTAAAAAGTTCAGTGGGAAAATTTTTCAAGAAAAAAATAATTCAAAAATTTAGTACTGGGGGGAGTGGTTTCCTGC	0BDDEDDDDEEEEEEDEDDDCDCDDDDDDDDDDEEDEEEFFFFFFHHHHHJJJIJJJJJJIFIGIHGJJJJIJJIIHHFJHJJJJJJHHHHFFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:3
+BS-DSFCONTROL03:121:D0M8KACXX:5:1307:16910:75714	165	chrIV	16922	0	*	=	16922	0	GGTCAGTTTTCAATTTTTCTTAGTTTTCCAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGCTTATCAAAAACAAACAGAACGAAAAAAATTTTTAAAAAAT	@C?DFFDFHHHHGJJJJJJJJJJIJJJJJJJJJJJJJJJJJHFDDDDDDDB#################################################	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:1307:16910:75714	89	chrIV	16922	60	100M	=	16922	0	ACGCATCGAGACGTATAAAATTTGTAAAAAGTTCAGTGGGAAAATTTTTCAAGAAAAAAATTATTCAAAAATTTAGAACTGGGGGGAGTGGTTTCCTGGG	<DDEDDDDDDDDDDDEDDDDDCDDBDDDDDDDDEEEEEDFFFFFHHHHFGJJJJIEJJJJJIIJIIJIJJIJJJIGJHJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:6
+EAGLE:1:116:7753:12206/1	0	chrIV	16940	60	101M	*	0	0	AATTTGTAAAAAGTTCAGTGGGAAAATTTTTCAAGAAAAAAATAATTCAAAAATTTAGTACTGGGGGGAGTGGTTTCCTGGGTCCCCTGAACATTTTATCC	EBE=EAEEEEFBFDEFFFFFFF;EFAFFDFFFFFDFFF@ECCCCACDFFFEFFFFFFFAFFEF=FEFFBC<FF@BCACBAEE=EBEE?CBEDD?EEA=B7C	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:78:11145:14387/1	0	chrIV	16952	60	101M	*	0	0	GTTCAGTGGGAAAATTTTTCAAGAAAAAAATTATTCAAAAATTTAGTACTGGGGGGAGTGGTTTCCTGGGTCTCCTGAGCATTTTTTCCACTAAAGTTTTG	GGGGGGFDDGGEGGGGGGGGFGGDFG@GFGDFGGGGDGGGFGFGEGEGFDGGFEGGCEE:@>>>CDDDED:CDCDDC?DEEEEEEEEBEEBBCCCEEEEE?	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:43:19134:12725/1	0	chrIV	16956	60	101M	*	0	0	AGTGGGAAAATTTTTCAAGAAAAAAATAATTCAAAAATTTAGTACTGGGGGGAGTGGTTTCCTGGGTCTCCTGAACATTTTTTCCACTAAAGTTTTGCGGA	FFFFFGEGGGFGGAGGGFGGGGGGGGGGGGGGGGGGDGGGDFEFFGGGGGGGAD@FFCDFGGEFGFEGFGGFGEEEEEGEGGGEGBGGEEBE@DBEBECEE	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:1:57:3806:20180/1	0	chrIV	16957	60	101M	*	0	0	GTGGGAAAATTTTTCAAGAAAAAAATAATTCAAAAATTTAGTACTGGGGGGAGTGGTTTCCTGGGTCTCCTGAACATTTTTTCCACTAAAGTTTTGCGGAA	CCC:;DD?BDEEDDEEDE=DECECCEDEEEEAE?EEDEE=ACACCBA=ADDA@;DEBCEEEEEEBBE:EEEEEEEBEDEDEEBEEEB?A@C5@@CC?BD?5	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:28:11193:2776/1	0	chrIV	16966	60	41M1D59M1I	*	0	0	TTTTTCAAGAAAAAAATAATTCAAAAATTTAGTACTGGGGGAGTGGTTTCCTGAGTCCCCTGAACATTTTATCCACTAAAGTTTTGCGGAATGTATTTTTA	GGGGGGGGEGGDFGGGGGGGGGGGGGGGGGDGGDFGGGGGGGG?GFCFFFGGGEGEGGGGGGEGFEGGGGEEEGFEEEEEGEEFGECEEA?CECCDEEECE	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:1:63:18448:7383/1	0	chrIV	16991	60	101M	*	0	0	AATTTAGTACTGGGGGGAGTGGTTTCCTGGGTCTCCTGAACATTTTATCCACTAAAGTTTTGCGGAATGTATTTTTAATTCATAGTTTTTGATTTTATCTA	FDGEGGEGGGGGGFDGGGCGGGEGGGGFGGEAEEEFFDFFFDGGDGGFGGGGGGGGFEGFFFFFFE?FEBBEEEEACEFDFFFAFEGFGGGBFBGDDDGCC	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+BS-DSFCONTROL03:121:D0M8KACXX:5:1213:7683:14705	73	chrIV	16997	60	100M	=	16997	0	GTACGGGGTGGAGTAGTTTCCTGGGTCTCCTGAACATTTTTTCCACTAAAGTTTTGCGGAATGTATTTTTGTTTCATAGGTTTTGATTTTATCTAATGGA	BBBFDDFF@DHHHDHIHIIJJIJJJCGHJJIHJJIIIJJIJJJIJJJJJJCEHIJIHHFEDDECDDFEEDBACDDDEEDDCCDDDBCDEDEDCDECDDCD	PG:Z:SNAP	RG:Z:000	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:1213:7683:14705	133	chrIV	16997	0	*	=	16997	0	GTTGAGGGCTATATCTTGTAAACTGGTTTATTTATAGAAACAAACAAGTTTTGGTCTGAAAATTTTGAAAAAATAATATCTCTTGGCAGAGCATTGCTAA	CCCFFFFFHHHHHJJJJJJIJJJJJJIIJJJJJJJJIJJJJJJJJJJJGIJJJJJJJJJJJIIJJGHIJJJJHFFCDFFFFEEEEDCDBBDDDDDCDDEC	PG:Z:SNAP	RG:Z:000
+EAGLE:1:53:14974:4822/1	0	chrIV	17018	60	101M	*	0	0	TGGGTTTCCTGAACATTTTTTCCACTAAAGTTTTGCAGAATGTATTTTTGATTCATAGTTTTTGATTTTATCTAATGGAAGATGAAGTTTCGTAACATTAA	AABA:=DDDDGF?GGGGGGEEFGGFEDE?E?DDDAE=EBEDEEDEEDEB@?DEDBEBECEEE=EAGDGD:FFCB?FDADEGAFGBFDAEFEEA?E?BAA?A	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:79:7894:12928/1	16	chrIV	17065	60	101M	*	0	0	TTGATTCATAGTTTTTGATTTTATCTAATGGAAGATGAAGTTTCGTGTCATTAACCATGCTCTGCCAAGAGATATTATTTTTTTAATTTTCAGGCCAAAAC	ECCCDCBAE=DEEEEEEEEEEEDDEE=EEGGEEGGGGEEGDFGGFGFEEAFCGBGGGEGGEGGGDGGEGGGGGGGGGGGGGGGGGFGFFAGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2114:3180:43522	73	chrIV	17244	60	100M	=	17244	0	GAGTACAATCCTGTAATAAAATTTTCAAAAATTCGTTTTCCCGCTGACTAATCAGTGATAAACGGTTTTCAAACATATTTTCTCAAATTGTCATTATTGA	BCCBDEFFHHHHHBGGGHIJJJIJJJJIGFFA<AGFGHIJIJJJJJJJEIHIJJJHIJIIIIJIJDEFHHHFHFFFFFFDEEEEDECDCC@CDECDDDCC	PG:Z:SNAP	RG:Z:000	NM:i:2
+BS-DSFCONTROL03:121:D0M8KACXX:5:2114:3180:43522	133	chrIV	17244	0	*	=	17244	0	TTATTATATAATCCTTAACCGTTCACATTTTTGTCTCATGGGAAGTAAAACAGTTCACTGGTTGTTACTACAACATTTATTCTCAGCTTCTCTCTACAGA	CEEDEDEDDDCCCEEDDDFHHGHAHIGIJIGFJIJJIEJJJJJIJIIIJIJJJJIIIIJIGJJIIIIJIHIJJJIJJJJJJJJJIJJGHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:1304:3598:47398	165	chrIV	17460	0	*	=	17460	0	GGCTTATACAAGGACTGAAGTTATATTTTGAAATTTTTCAGCTGATATAAGTGAAAAAAGGAAGCATAAATTGTTTTCGCAATGTGCCCTTTATGCTCGA	CCCFDFFFHHHHHJJJJGIIHGIJJJIJJDIJJJJJJJEHIHJJIIIJJJIHIJIIIIIIIIJJJJEGJJBHEEEAEHE9AACCACDDDDDCDBACDDD?	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:1304:3598:47398	89	chrIV	17460	60	35M1D28M2I35M	=	17460	0	GTTGGCTCGATTCCCCCAACTGACAAATTTTTTTTGGGTAATTGGGATTCGAATAGAAATGCTCGATTTTAAGCAAAATATGAGTGGGTCTCCTCTCCTC	BDDDDDDDDB@5B@DCCDCDEDCCCCDBDEHEHEJJIHGGIGHGHEHGEBIGEDGGIJJJIGIHIGJJIDJJIIJJJHBHEGGGIIJHGHGHFFDDDCB@	PG:Z:SNAP	RG:Z:000	NM:i:5
+EAGLE:1:76:16063:11662/1	0	chrIV	18079	60	101M	*	0	0	AATATACAAGAAAGAGCTCTCTGATGAAAAAATTGACCCTGAAATAAAAAAAATAGTAATTTGGTACTTTGCAAGGAAGACCTACTTGATATACTGACATG	GGGGGGGGGGGGGGGGGDGFGGGGGGGGGGFGGFGEGGGGGGGGGGGGGEEEEDEGEGGGBFFFCGGEEGGGEEEGEDGEEEEEGEEEEEEEEEEEEEEED	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:58:12937:19702/1	0	chrIV	18223	60	101M	*	0	0	AACATTAAAACTGTCTTTATCTGAAACATTAAAAGATTGGAGTAATAAAGAACAATGTGACGGATATATTCAGTCATGATTTGATTACCGACTGAGCTGCT	EGGEGEGGGGGGGFGGEGGGGGGGGDGEGGGGDDGCGGGGGGEGFGGFGFGGGGGGGEFFGDGFEFGGGGGFGEGGGGEEEGGFEGGGFGEEGGEF=AGBD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:42:10933:8029/1	0	chrIV	18242	60	101M	*	0	0	TCTGAAACATTAAAAGATTGGAGTAATAAAGAACAATGTGACGGATATATTCAGTCATGATTTGATTACCGACTGAGCTGCTATTACCGACTGAGCTACTT	FGGGDGGGGGGEGAGGFGGGGGGEFDGDGGFEGGGGGGEGGGGGGGGFEGGGGGEGGGGEFGGD=DCDFFGFGEFEGFEGFGEGBGDFGEBGEDEDEEEEG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:57:16376:15904/1	16	chrIV	19300	60	101M	*	0	0	ATCTCTTTTCTCGTAACTGCTGTAAAAAGGAACGAAGCACAGCATATAGGCAAACAGAAAAATGAATAAGACTCGGAAGATCTGGAAATAACATTATTATT	:DFBFEDEBGFFEGGEGEGGGGDGG?FGEEDEGGBGGDGEGGEGGGGGGGGGEGDGGGGGGGGGGFGGGGEGGGGGGGGGGGGGGGGGEGGGFGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:82:4792:16460/1	0	chrIV	19447	60	101M	*	0	0	ATCAATGATTTGTTACAAGAAATTTTAGAATTTGTCCGACACAATTCTTAGATTTTTGAAAATACTGTTCAAACATTATAAAAATATATTTAAGTTGCACT	DGGGFGBFGGDGGGFGGFGGGGGGGGGEFGGFGEEGGGFGFFDGEGGGCGGGFGGGGEEGGGEFGBFAEFGGFGEEEFEEEEEE>C@EDEEEGEECEF=@C	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2112:12255:61856	99	chrIV	19979	60	100M	=	20074	195	TCTTGATACTGCATGAATTTTCTTTCTGCCAAAAATGAAAAAGTTGAGGCATAATCAGCTTTCAATGCTTCCATCGCTTGACGCGGTCCCTTGACTCCAT	BBBFDDDFHHHHHJIJIIIJJHIJJJIGIJIJJIJJJJJIJJIGHGJIJJGHHIJIJGBGIGIIJJJJJJEHHJJJHGHEFFFFDDBB;BCCDDDDDDDD	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2112:12255:61856	147	chrIV	20074	60	100M	=	19979	-195	TCCATTCAAGTTGAGATGATTGATAACTTTACACGCGAGTTCTTCAAGTTGTCTTTAAAATCAGAAATGGCCGTACACTTGCAAACGAGAAAACCTACTC	AC@DDDDDCDDDDDDDDDDDEDEDCDCCCCAEEGJJJJIHEJIJJJJJJJJJIJJIJJJJGJJIJJJIJJJIIIHHCJJJJJJJJIJHHHHHDFFDBC@B	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2307:13742:62661	99	chrIV	20173	60	100M	=	20424	334	TCTTGAGTTGAGCGTAATCATTGCGCTTTAAAACTTGCCCGAAATCCTTAGCGTCTAATAATCGTTTCATAGAATGCGCCACGAAGCACATTACCATTAT	?@@DDBDADDHFBE<CEBHBHICH?FAEH@@?CGH@F<9BFEF@HGECEGI>B';AEFEFEHC?;B566>;;>6>;5'9''3;?5?9CCCCCACCC>:A3	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:2307:13742:62661	147	chrIV	20424	60	17S83M	=	20173	-334	AAAAAATGACGACACCAACACTTTGAATTTAGTACGTACTCTTGCTTTTTGCATTGCAAATTTTTCTTAATTTGAATGTTGAGTTCATCCTTTCTACAAT	#################@;)CHC;CC=:ED@EECHDCB2G>F?(0)?9?9E??:IHDH?);HE9BEFBH@IHFA<3CFFA3322++<++:<HFDB=BBB8	PG:Z:SNAP	RG:Z:000	NM:i:1
+EAGLE:2:75:4143:14945/1	0	chrIV	22397	60	101M	*	0	0	GTTCAAATCAACCAAAGTTCAGTTACGCAGTGTAAAACTGGATTTTGACCATGGTGCATCTATTGAAAGTTTGTATATTGTCTCACCAAAAAATCGTTTCA	GGGGGGDGGGGGGGGGGFGGGFFGDFEFAFEFFCFGGGGGGFGEGGAEGGGFGGCFFFEFGEEGGEEG?@EFADEFGEEFEFEGEEFEEDFDCBEE>C=E@	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1207:8708:89857	99	chrIV	22773	60	100M	=	23094	421	ATACAGTATATTAGTTTTTAAAAGTTTTGTGCCAAACGAAATTTTGTCGAAAGTTGACAACTTTTTTGTCTAGAATTGGCAATTTTTTAAAAATTTAAAT	CCCFFFFFHHHHHJHGIJJJJJJJJJJJJHJJJJJJJJJJJJIJJJIJIIHIIIIIJJJJJJJJJJHHHHFFFFFFB@CEE>ACCDDDDDDDDDDDDEDD	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1207:8708:89857	147	chrIV	23094	60	100M	=	22773	-421	GTTTTTGAATTGAAATTGAAAGAAATCGAGCAACTTGAAGCCGAGGCCGAAAACACAATTCCAAAAATACCCAAGAAAGCAGCTGGCTCATCTTCAGAGC	@DDDDCCEEDDCDEEDCDDDDDBCA<DFFFFFDHHHHHJJIJJJJJJIJJJJJIHJJJIGJJJJJIHF?JJJJJJJJJJJIJJIJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2108:16786:19522	99	chrIV	23918	60	100M	=	24045	227	GTAATGTACGTATCAAAAATTTTTCTATTATATATTATTTATAGTAATTTGTTTGCGACCCTATCGTATTTTAATGAAGGATGTAAAGTAACAGACTACA	BBCFFFFFHHFHHJJJJJIJJJJJJJJJJIJJJJJJJIJJIJJJIICIIJJJJJJJJJJJJJJJJJHIIJJHHHHHHHFFFFFEEEECCEDDDCDDDDDD	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2108:16786:19522	147	chrIV	24045	60	100M	=	23918	-227	CCATTTTGCAAACTTTCAGTAGAAAATTTTCAACTTTCTAAAGCACAACTTTGAGTCGATAACAAACAAAAAAGATATCCCTCATTTATTTTTCCACACT	ADDDDCDCCDDCDDDDDEDEDECDEFDFFFFHHHHHHJIJJJJJIJGIHIJJJJIJJIJJIHJJIIIJJJJJJJIIIIJJJIJJJJJHGHHHFFDADBB@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:49:4402:14093/1	16	chrV	2002	60	101M	*	0	0	ATTAGTCTTGCATGCAAGACTAATTTTCAATTGACCCGTAGGGGTGCAAGACAAATAGGGGTGCAAGACTAATAGAGGCTGCAAGACTAATAGAGGAAATA	DBEDEEDCCEA@DEDDEGEEGDDEED=FEDGEFBEDFGFEGGGGGGEGFEECGFGGFFGGGGGFGGGEGGGGGFGGGG=GGGGGGGFGGGEGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:42:2639:3086/1	16	chrV	2123	60	101M	*	0	0	GCGATAGCATTTCGAGAGAAAAATATTCTTAAATGTGCTCAATATGGCTGATACAGATAGGTTTTTCATTACAGACAGGTTTTTCATTTCAATTTTAAAAA	EBEEEBEEB:BEBEEA;A=EEBE?CEE?EEEEED?D?EEE:DDEEEEEEE=DAD:DEE=DEBEEAEEEDEEEBDEBDEEDEAEEEEGGEGFGGGGD;FD?F	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2310:20321:98700	99	chrV	3178	60	100M	=	3335	257	GTCTATGAAAATGCTTTTATTTGGTATTACAGTTCTTTTCAAAGGCACACATATTTTTCCCATTTAACAAAAAATCGTCGTGACCTTATATCGTATTTTC	CCCFFFFFHHHHHJJJJJIJJJJGCHIJJJJJIGIJJJJIIJJJJJJJIIDHIJJJJJJJJJJJJJJJGHJJJJJGHFFEDDEDECDDDDFEDCDDDEEE	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2310:20321:98700	147	chrV	3335	60	100M	=	3178	-257	GCAATAATACAACACCGGGTCTCGACGTGGCAAACGATTGTTTATTGATTTTCACGGGATTCTCGCCTTTCTCATTGAATTTTCGCGCTCCATTGGCAAT	CDEEDEDDCDD@@<DDDDDDDDDBBDDDDCDDDDDDDDDDDEEEEECFFFFFHHHJJJJJJJJJIJIJJJJIJJJJJJJIJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2201:16390:41463	99	chrV	3761	60	100M	=	3998	337	AAATAGAATTCATGATACACGATCTTGAGAAGAGCTCTGTCCTCCAGGCTGCGAACTGGAGCGTAGAAAGTTTTCATATTGTTAAAAACTGCAAAGAAAT	CCCFFFFFHHHHHJJIJJJJJJJJJJJJIJJIJJGIIJHHIJJJJJJJJIHIJJJJJJJIJJHHFFFFEECEEEDDEEFEEEDDEDDDDDDDD@CCC1?>	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:71:9405:4095/1	0	chrV	3773	60	101M	*	0	0	TGATACACGATCTTGAGAAGAGCTCTGTCCTCCAGGCTGCGAACTGGAGCGTAGAAAGTTTTCATATTGTTAAAAACTGCAAAGAAATGTTCGATTCCTTG	DA=DADDBB:;?@@@=DDDDDDD?DDDBDDCDDDDDDDDDDB?C=DDADDD=DDB?AA:ADCDDCCBCDB=:@ACDBCA=ABCBDB@>A@ABC??BB=BBB	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:65:16164:2544/1	16	chrV	3846	60	101M	*	0	0	AAACTGCAAAGAAATGTTCGATTCCTTGTTCTGACGTGTGATGCTTTCTCAAATATGGAATTAAATGACAGACAAGGAGATGAAGTTTCGGAGTGACTCAT	@?@C@B;>EDDBDDBEEEABBA@B=C:EFEFFDBFEEEEEEADDCBEDBDBFD5FFFFFBFFFEBEFEAFFFAFFFFFFFFEEDEE?D=GGGGFGGGFGFG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1214:8214:10158	99	chrV	3997	60	100M	=	4220	323	GTTAAAGCAAAACTTCAATTTTGTCAATCTCTTGGTCTAAATACGTTCTATCGTTAGATAAATCTAACTATCTAACTGAAACGAGTACAACTATATTGTA	C@@FFFFFHGHHHJJJJIJJJJJIIJIJJJJJJJJFHIJJJJJIJGIJJJIJJJJJJJJJJJJJJIJIIIJIIIIDGDHHEHGFFB>CCCEEDDDDEEAC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2201:16390:41463	147	chrV	3998	60	100M	=	3761	-337	TTAAAGCAAAACTTCAATTTTGTCAATCTCTTGGTCTCAATACGTTCTATCGTTAGATAAATCTAACTATCTAACTGAAACGAGTACAACTATATTGTAA	DDCCC>DC@ECEEC@EEFFEFHHE>HGIIIGHCEC=/)FIHFHIJIIGIHIJJJIHIIIJJIIIIGGJIJJJJJJIIJIHIJJIIGGHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:1214:8214:10158	147	chrV	4220	60	100M	=	3997	-323	AAAATTAGTTTAATCTCCTCGCAAGATTTTTTTCTCCGAAATTTAAGATTGGGCGCTTAGTGATATCATAACTCTGCAGCCATCAGAAAAACTGAAATGT	CDEDDDDCCDCCA@BBDD@BC>CCDB@CEFHHFIJIJHIIIGJIJIIIIHJJIJGHIIJJIGJJIJIHGHHGFJJJJJIJJIEJJJJHGHHGFDFFF@@@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:76:4095:13262/1	16	chrV	4714	60	101M	*	0	0	ATCGTAAGGCGGTACTTTTTAAAATTTCGTTTCCATTCTTCCCCAGGGATCCCGTAAAAAACCAATTTTTCGACTAGGTCTCACATTTTGCCCTACAAGAG	B=BC:CACBCACA=ECD:CDGA?AA:ADA:A@EEEBCGDAGGGGEGGGEDGGGGFFGGFGBFFFFFFGGEGGGGEGGGGGFGDGGGGFGGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:89:14779:18797/1	0	chrV	4998	60	101M	*	0	0	TGTGTTTGGTTTCATTCAGACGCTACTTGCCTGATCTATCAGTCTAACAATTTTTGTTTAATTTTCTTCACTATCCGCCGTTTATAGTTCGTTCTTCCGTA	EEE=FEFEEEFFGGGFGGFGGGGDGGGGGGGFD?DFFEFCFGDGGEGGEEGGGGGGEGG=FGGFFFGEEGGFEGGEGGGGEGG@EDDADDEEEBGDDEDCE	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:1302:16740:19201	99	chrV	5929	60	100M	=	6103	274	CTCCAGGAAGACGAAAATTCCACAGACTGAGTATCCACGTAAGTATACGATTTTTCTATGCGCTATAAAATTCTTTAAAATCCGCTATTTTCCAGCTGAT	CCCFFFFFGHGHGJJJJJJJJJJJIIJIIGGCGIIJIIJHIJJDHIIJJIIIJJJJJJJJJJJHGFFFFFFFCEEEEEEDEDDDDD=BDEEEDEDDDDCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2115:16987:87976	99	chrV	5959	60	82M18S	=	6130	228	GAATCCACGTAAGTATACGATTTTTCTATGCGCTATAAAATTCTTTAAAATCCGCTATTTTCCAGCTGATTACGCATCTAACGACCTGATGAACAGTTTC	1++=+2+22+2A:HD?E,A+++32+++2A@*:8C):BBGD4*9B9*?*099/?6(6@'.@E;)).==)7;7?),5;ACA53@##################	PG:Z:SNAP	RG:Z:000	NM:i:1
+EAGLE:2:85:10419:2317/1	0	chrV	5963	60	101M	*	0	0	CCACGTAAGTATACGATTTTTCTATGCGCTATAAAATTCTTTAAAATCCGCTATTTTCCAGCTGATTACGCATCTAACGACCTGATGAACAGTTTCAATGG	GGGGGGGGGEFFFFFBDGGGGGGGGGGGGGGGGGEGGDGGGGGFEFGFEFEEBEGFDGEEGEGGBEGEBGGDEEEEBEEE?DCDCEE?EECE;CCACCCC?	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1302:16740:19201	147	chrV	6103	60	100M	=	5929	-274	CACCTATGCGTCACGCTATGGTCCCGCATATCTTCGCGAACGATTCGGAAGCCATGATTCCCAACTTGAGCGTATGCTTTATGGCGGCAGTGGACCACAG	@:CCDDBBDDDB<BDDDDDDDDBBBDCC@CDDDDDDFFFEHHHJHIJJJJIJJJJJIGHJIGIJJJJJJJJJJJJIJJJJJJJJJJJHHHHHFDFDFCCB	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2115:16987:87976	147	chrV	6130	60	43S57M	=	5959	-228	CACTTCGCGAGCTTGCCCCCTATGCGTCACGCTATGGTCCCGCATATCTTCGCGAACGATTCGGAAGCCATGATTCCCAACTTGAGCGTATGCTTTATGG	###########################################D@;'/FFF;-(/8(0)B:):9:)8:??*1?DG@C<A<:IEE?@;DBAC?DDDD?@?8	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1213:12756:79582	165	chrV	7559	0	*	=	7559	0	GATGAGGAACGAGCCGTCGCGTCCTTGGGAACTTCATATCTGGAAATCATTTACAAAGATAGCTTATAATAAAATTGAAAAATTTCCTCATAATAACAGT	:+1++2+++<)<0B<C2@D)1:0889B3(8?#####################################################################	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:1213:12756:79582	89	chrV	7559	60	100M	=	7559	0	GTGATTGTACAGCGACCAGAAGTCCTAGACAATGTCGAAAAGTGAAACCAACTGGAAAAAAACGATAAAAGGCGTATTTAAAAATCTTCTTCCTTCAGAC	########################################################?);?00:*?*DC:):)FA,CBA3A:C<<A2;:<2+B?;A7B?:?	PG:Z:SNAP	RG:Z:000	NM:i:6
+EAGLE:2:47:13418:12913/1	16	chrV	8644	60	101M	*	0	0	CGTCGTCGTCAGGAATTCATGAGCCGTCCCGGTGTCTCGAGCTCTTGTGAGCATACACACAGACACATACAGCAGCAGCCTCCACAACATGCACATATAAA	A=6=857::?9AAAAB?????8@=:>5A:?BADADA???5@DA:EEBEFBFBBBACAEA?EEBCBBBABEEFGBGGGFGGGFFEFFDBGGGGFDGFGFGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:47:2986:6932/1	16	chrV	10038	60	101M	*	0	0	GAAGATCTAATAGCTTTGTAAAATTGATAATTTATCGTCAAAAATTTGACTTGAAACAGTCTAAATCGTGCAAAATACATACTTTTCTATTGTTTTATCAA	EEEEEEEEEEEBBFEBGEEEGEFF?EEGEEGGGGEGGGEGFEEE?EFFCFFFF=FCGFGGGGFGG?GGGGGECCEDEGGFDGDGFFGDGEFGGGGGFFGGF	PG:Z:SNAP	RG:Z:266-1	NM:i:2
+EAGLE:2:104:13973:5102/1	0	chrV	10071	60	101M	*	0	0	ATTATCAAAAATTTGACTTGAAACAGTCTAAATCGTGAAAAATACATACTTTTCTATTGTTTTATCAATTATTTGAACACTTTAAAATTTAATTTAGCTAA	FGGGGGGGGGGGEGGGGGEGGFGGG=EFFFEGGFGGGGBGGGGAGGFFGGGGFGGGGGGEGGGGFGFGGGGGGG?DDGEGGBGFGGFFGAGFGGGGFFGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:113:10088:6922/1	0	chrV	10199	60	101M	*	0	0	CAAAATGCTGCTGGTCTGGCAAGAATAGGGCTGCAAGACTATTACCGTATTTCCTCTATTAGTCTTGCATGCAATACTAACAGAGGCTGCAAGACTAATTT	GGGGFGGGGGGGGGEGGGGGGFFGGGFGGFGGGFDFGEGEGDGGGFFDBFGEGGEE=E?EFGBGBEEEEDFCEEEEEEBDGEE?EEE@ED:@B59ADDACD	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+BS-DSFCONTROL03:121:D0M8KACXX:5:2313:7454:29242	99	chrV	11315	60	100M	=	11637	422	GTCTACGGGAGGGGGATCCTCTTGTGTAATACGTTATTAAATTATGCCTGTCAGAGCAGCAACACACACAGACAAGTGAGTCGTCGACTCAAAAAAAGAA	CCCFFFFFHHFHHJJGIHIGIJIICHGGIIJJGEHIIJGGIDHIJGIIGIHHHCEHFFFFFEEEDDDDDDBDBDCACDCDACBBDDDDDBDDDD8>>5??	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2313:7454:29242	147	chrV	11637	60	100M	=	11315	-422	ATGCGGGAGGAAATCGTAGACATCTCAACTGATTTCGCATGGTTAAGAGTGTGCTGACGTCACAATTTTTCTGGGAAAAAATTCCGCATTTTTGTAGATC	@DD@BCDDEDDB?=BDEDDDDDDDDCDEDDDDDDFFDDGGHHHHJJJIGHIJJJJIIGGJJGIJJJJIIJJJJIJJJJJJJHJJJJJHHHHHFFFFF@@C	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1303:5368:22825	73	chrV	11689	60	36M1I5M1I17M40S	=	11689	0	GGTGACGTTACAATTTTTCTGGGAAAAAATTCCCCAATTTTTTGCAGATGAAACCGCAATGAGACTTTCTGATAAAACGTCAGTACTCCACACGGCCGAA	++:+AD7D<+A,++AFE:F:,+++2++<:DB4:?)))0*000?'=8=/CFC;@@6@:A6B########################################	PG:Z:SNAP	RG:Z:000	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:1303:5368:22825	133	chrV	11689	0	*	=	11689	0	TTTCTCAAAAATGTCACGTCAGCACGTTCTTCATAATGAAAAATCAATTGAGAATCCCGCATTTGTTTGTAGATCTCCGTAGATCAAACCGAAATCGGGC	################################@@DAA>D?@??;)==.)==.7E@8FD@FFFB>BBB::0?01))1)?CF<94BE?E<<FDFDD=?D@@@	PG:Z:SNAP	RG:Z:000
+BS-DSFCONTROL03:121:D0M8KACXX:5:2202:14381:50677	99	chrV	12587	60	100M	=	12708	221	CTCAACATCCTGAAATAAGTTTTTGTCAGTTTGGTCTCCATTTTTTTGTCTGCAAACGATAAAATCTAAAGATCCAAAAATAAAATTAGTTAGTTTTAAC	CCCFFFFFHHHHHJJJIJJHHIJJGGIIJHIJJIFHIJJIIJJJJJIJIJJJGIIIGIJ?EEFHGGEFFFFFDCC@CEDDDDDDDEDDDDDED>CDDDDD	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2202:14381:50677	147	chrV	12708	60	100M	=	12587	-221	TTAAAAAAAACAATGTGACTTGACATCACCGTCTGTGTTCGTCTAATGAAAAACAGCAGACCGAAAAAAAAACGGGTCTGTTTTAATTAAAAACTGATAG	:1DB@DCC>@>AADCCA@CC>:>>>22&902?A<?@B@@DAC>:E@;;D@FEEHE>@GEDF;;JJIIGGGGEIJJJIJJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:100:2124:19174/1	0	chrV	13308	60	101M	*	0	0	TTTGATTTTCCTCTAGAATGTTGCTTCCGTCTCCTCAATCCATTTTCATTCCACATAGATTCTAAAACAGAAAAAAATCGATTCAGAAAAAAGTTAACAAA	?EEDEAFFFFFFGEGGAGDGGGGFGGGGGDEGGGDGGGDDE5EDEEFEAFGGGDDB?GGFGFGDDGGFGA?GGBFCDAEAGEEFBAED?EA>A=ADDAEEG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2305:14519:87038	73	chrV	14262	60	100M	=	14262	0	ATATTAGGTCTCCAAATAAGTTCCGGGTCAAAAATCATAACTTTGTTCGCTGCGTATCGATTTTTATGAAATTGTGGGAATTTATGTTATCAACCATGAT	CCCFFFFFHHHHHJJJJJJJJIJJJJJHIJJJJJJJJJJJJJJJJJJJJJJJJJHIJJJEHHHHFFFFEDEEEEEABDBDDDDDFDFEEECDDDDDDDDC	PG:Z:SNAP	RG:Z:000	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:2305:14519:87038	133	chrV	14262	0	*	=	14262	0	TTCATTTGACAATACTCACAAAATTTTTTTGCCGTCCGAAGTGCCCTAACTCGGAGCCAAATTTTTCAGGCATTTTTCAGATCTCGCTTCTTTTACGCTT	DDDDDDEEEDEDC@DDDDDDDDDDDDDDDDDDDDFFFHHHHJJJJJIIGFIJJJIJJJJJJIJJJJJJJJJJJJJJJJJJJIIHJJJHHHHHFDDDFCC@	PG:Z:SNAP	RG:Z:000
+EAGLE:1:73:5284:8999/1	0	chrV	14265	60	101M	*	0	0	TTAGGTCTCCAAATAAGTTCCGGGTCAAAAATCATAACTTTGTTCGCTGCGTATCGATTTTTATGAAACTTTGGGAATTTATGTTATCAACCATGATCTTT	GGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGGGGGGGGGGGEGCGGGGGGGGGGFFDGGFGGGDGEDBGGGGGEGGGGCGGGGGGEBFFGF	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:83:17647:3047/1	0	chrV	14268	60	101M	*	0	0	GGTCTCCAAATAAGTTCCGGGTCAAAAATCATAACTTTGTTCGCTGCGTATCGATTTTTATGAAACTTTGGGAATTTATGTTATCAACCATGATCTTTCAT	=E?DEDE?EDBDDD=DBDBDAAAACDFFFFFDEFF?CDD@DE=?BAA??A=EEEE?FFFF@EFFDBFBEFFEDF=BE?BE=@C?CBE@EEEEBE@EEADDD	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:24:16337:8220/1	16	chrV	14278	60	101M	*	0	0	TAAGTTCCGGGTCAAAAATCATAACTTTGTTCGCTGCGTATCGATTTTTATGAAACTGTGGGAATTTATGTTATCAACCATGATCTTTCATTTGACAATAG	=DD@ECEBCEC?DFGGFFEFFEE5DEFEDDDGEGGGDGBGGGFFGGEGFGFGGGEGAGGGGEEEE?DGGGGFGGFGGGGGGGGDGGGAGGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:7:10444:9975/1	16	chrV	14287	60	101M	*	0	0	GGTCAAAAATCATAACTTTGTTCGCTGCTTATCGATTTTTATGAAACTGTATGAATTTACGTTATCAATCATGATCTTTCATTTGACAATAGTCACAAAAT	BBEEDEEBEEDBEE:E=EEEEAD:BD?EEAEEEBABEEDBDDDEA?EEDEADBDBDDEBEBEE?EEE==EECA=;=DDB?AEBC=EDDD=DE:EBEDE=AE	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:59:15165:16923/1	0	chrV	14304	60	88M1I12M	*	0	0	TTGTTCGCTGCGTATCGATTTTTATGAAACTTTGGGAATTTATGTTATCAACTATGATCTTTCATTTGACAATAGTCACAACATTTTTTGACCACCCCAAG	EGFGGEGDEDFFFBFGGAGGFGGGG@FFEFGGGAGGEFDGGBGGGG=GEFGFGCGAGFEGGFBGGCEEEE?C@BBAFEEFFFEBFFGDB?=FEDEGEEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:42:11999:2677/1	0	chrV	14317	60	75M1I25M	*	0	0	ATCGATTTTTATGAAACTTTGGGAATTTATGTTATCAACCATGATCTTTCAATTGACAATAGTCACAACATTTTTTGACCACCCCAAGTGTCCTAACTCGG	GBGFDGGGDFAECFEFFGGFFEFDFEFF?EGAFGGGGGGG=EBBBEFFFFABCC=EDFEDEE@EBEFEEDEEEDGEB:?EEEEB=8BB:E5AA@:@@AAAD	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:2:11:9361:9333/1	16	chrV	14441	60	53M1D48M	*	0	0	CACGGCAAATTCGGCAAATTTGCCGTGCTTAACAAACTCCAAAAAATGTGACATTTTTTGATGTTTTTTGGAGCACCAAAACTACTGAGTTCTGAACAAAA	=CEEDBGFGEBGEEFGEGDEEGGGGDGGGDFEGGGEGFGGGGGGGGGGGGGGGGGE=FGGGGGGFGDGGGGEGAEFGGGGGAGGGGGGGGGGGGFGGGG?F	PG:Z:SNAP	RG:Z:266-1	NM:i:7
+EAGLE:1:82:11123:16509/1	16	chrV	14444	60	53M1D13M1I34M	*	0	0	GGCAAATTCGGCAAATTTACCGTGCTTAACAAACTCCGAAAAATTTGATACTTTTTGATGTTTTTTTGGAGCACCAAAACTACTAAATTCTGAACAAAAAG	BCBCACB=EEEAFGFEDFDFGGFFGFFF?FFEBEEADGGDGGGGGFGGGEGBDDGGGF@GDC@C@@5EFFFGFEEGDFFFFEFDFF=EEEBCCCEDGFGDD	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+EAGLE:2:120:10149:2029/1	16	chrV	17145	60	101M	*	0	0	TTTTTTTTGTTGTTTTTTTGTTTTGCGAAAAACTTTTGAAAGTGTGATCCCACGTTCTTTTCTTGTCTGCGTCTCTTTTAAATTTGGGTTCTCTCGATAAA	<C<8;AD?ACCC?ACA:AEGFEGDFFBFFFFBEEAAEDDDBGGGEGBFFEFCEAB?FDFGFGGGFAFGDGGGFGFGGGDDDE?FGGGEEFDFFFFFBCAEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:3:16562:7761/1	0	chrV	18570	60	101M	*	0	0	ATTTTCAACTCCGGCAAATTGCCGATTTGCCGTTTGCCGGAAATTTTTAGAGAAATTTTTATAAGACGGAAGCATTTAAAGCTATATCATTCGGAAGAATT	GGFGGGGGGEGGGGGGGFGGGGGGGGGGGGGGGGGGGGFGFGGGGGGGGEBGEGGGGGGGGGF=EEFGGFGGFEGGGGFGGGGEDFGEEGEFGFEBGDDFG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:88:4071:9423/1	0	chrV	19829	60	101M	*	0	0	ATTGAGTTTTTCTCCCCATAAAAGAAGCGTTGACTAGGAAAAATGAATGCAATTTTTTAGTTAGCAGGTACACATTATTATTCAAAAGTATTTAGAAGTTT	EFFFFBBDEECDB>?DDCDDADEE?CCED:DD5DDEF?FE?DEAEFFF:F??:DDFDFDFFF==DCBEEEB?EDEFD=DEFBFDFFFF?FFAFDFD:EB5C	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:42:18060:2997/1	16	chrV	23388	60	101M	*	0	0	TTTTTCCGATTTAATTTTTGATAGGTTTTCTCCGCAAATTTTCCACTTCAGTGCATGGCACACAAACGGCATGTTATAGTAATTTTCCAATTTTCCAAAAG	FDDECFADDB@FEEGED?EGGGFBEGFG?GEGGFGGFFGFGGGDCGGCGGEFGGDGGGFEGDFFFDGGGGEGGGFFGGGGDGGGGGGGGFGGEGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:13:6904:13626/1	0	chrV	24494	60	101M	*	0	0	GGTTTTTCTTGAAATATCGTTTTTAAGGTCAATTGATATGATAATAAAATATATTGTTGACCTTAAAACACATTAAAAAAACTTCGACAAGAACCGACAAA	EDEDABDA8BCF=FF=EEEDFFDFFFDA=FFEDEFBFADF:EDEED=FEEEFFD?DAEEB=DEDEFEDFCEABEEFFE?B8CDBFAFDFFFEFEBFEFEDE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:47:11060:17697/1	0	chrV	24606	60	101M	*	0	0	CGGTTGAAAACGATAAAGATAAAACATTTCGTAAATATACACAAATCTCGTAAATCGACACATGGCGTTTTTGGCGCGAAAATTCGGCGTTTGAAAAACTT	GGGEGGGFAGGGGADGFDDGGGGGGGGGFGGEGEGDGGGGGGDFDGGGFEDGGGFGEFGGGGBF=EE@BCFEFFDFEFEEEEEEGEBAFB=E==BFEDE@B	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2112:2411:63807	99	chrV	24799	60	100M	=	24916	217	AAGAGTACCTAACCAAAGAGCGACTCATCAATTTTCTGAATGAAGAACAACGTGATCCACGACTCAACGAGATCCTCTTCCCATTTTTTGATTCTCAGCG	@BCFFDDFFHGHHJJJJJJIJJIIGIGGGHGIJJJJJIIJJJIJJJJJIJJICGGGHGIEHGFHFFFFFDC>BDDCDC@CCCCCDEDDDDBCDDDDDD<@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2112:2411:63807	147	chrV	24916	60	100M	=	24799	-217	AAAAACACGAAAACGACATAAAATATCAAGAAGATGGAAAAATGTCCGGTGACGGTTTTCTCCGATTCCTGATGTCAGACGAGAACCCGCCAGTGTTTCT	?CC?8<<-:BB<?AABA>4DC@::A@@CCAC>:44>(DDDC@5=;??@;;;;8?;)B6/:55()@=)@8)==.))6DB6?990B?JJHHGHHFFFFF@C@	PG:Z:SNAP	RG:Z:000	NM:i:1
+EAGLE:2:45:11954:18961/1	0	chrX	1563	60	76M2I23M	*	0	0	TCACAAAGTTGCAGCTCCGAAAAGAGAATCCGTCTGGAATTCTGGAAGTTCACAAGAATTGTGAGTACTTACCTTTTTCAAGTGAAACTAGAGGAAAAATT	GGGGGGGGGGGFGGGGGGGGGGGGFGGGGGG=GGFGGFFGGGGFGEDFBDGFGGGGAGGGFCFEFCDDEEGGGFGFGGEAFFBFCEEFEDDCDGAADEBDB	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+BS-DSFCONTROL03:121:D0M8KACXX:5:1311:7062:60667	99	chrX	5712	60	100M	=	5891	279	AATAAAGTCCTGCCGCGAATGCTTCAAGGTTACTGTAGTTTTTGGTGGTGAGACCCGGAAATTTCTTTATTTTTCAAAAATTTCAGAACCCGGTCATGAT	CCCFFFFFHHHHHJJJJJJJJJJJJJJJJGIJJJJHIJIIJJJJIHIJAGIJIHHHFFBDDDDDDEDDDDDEEDDCDDDDDDDDECDDDDDDDBBDDDDC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1311:7062:60667	147	chrX	5891	60	100M	=	5712	-279	TCTTGGTGGGTCCCGTCACGAATTTTTGGAAATTATAGTAAAACGCCAATTTCATACTGATTTTGATGAATTTTAACAATAAATTAGCAGTATTTTAAGA	DBBDDDDDDDB>DDFFFFHHHHJJIGGJJJJJIJJIJIJJJIHHJJIJJIJJJJIIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1306:15859:17118	99	chrX	5939	60	100M	=	6055	216	ATTTCATACTGATTTTGATGAATTTTAACAATAAATTAGCAGTATTTTAAGATTCTGAAATTAATTTTTTACTGAATTTGGCGAAAAATTGCAAATTTTT	CCCFFFFFGHHHHJJJIIGGIGIIJJCHHJII@HHIIJIGJJHIIJGICB?GGHIIJGHHJBGEIFIHHJIJJJIJGIJ;@CHD<><?ACCDCACC>@@A	PG:Z:SNAP	RG:Z:000	NM:i:2
+BS-DSFCONTROL03:121:D0M8KACXX:5:1306:15859:17118	147	chrX	6055	60	1M1I3M1D95M	=	5939	-216	AACCTGGTGGTTTTCGTGGCGGGACCCGGAAATTTCTGAATTTACGCAAACCTGGTCATGATTGTACTTAAATGAAAGCTCCTAGCGAACTGAAATCGAA	@CBA@BDDBDDBBB=BDBBBAAADDDDHHGHEGIGIJIIIJIGGGEHHF?IHJIIIJJJJIJJIIIHHDIIIHIIIGJIIJJJJIIHFFHGHFFDFDCCC	PG:Z:SNAP	RG:Z:000	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:1210:6622:23752	99	chrX	6879	60	100M	=	7127	348	TTTTCAAATTTTTTTGGAAGTTTTCCAAAAAATCTCTGTTTTTCCCGCCGCTCAAAACCCTCAATTTTTCAGATTTCCCTGCTGGAAATGCTCGATTGCA	CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJIJJJJJIJJJJJIJIJJJJEFFDEEEEDDDDDDDEDDDDDDCDDDDEDDDDDDDDACCACCCBBDDDA:	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:77:8575:6989/1	0	chrX	6976	60	101M	*	0	0	GCATTGAAATTATCACTGAAGAATTCAAATCTCCAAAAATGACGTCTCCGTTCTCATCGCCATCTACCCGACAATCAATCACTTATATTCAAGCGAAAATC	GGGGGGFFGFGGGGGGGGGGGGGGGGGFCGGGGGGFBGFGGFGGEGEFGFEGGEGGGGEGEEEGEEEGGECD@CCFCD@FAECC@CAACECDBCDA?CCCB	PG:Z:SNAP	RG:Z:266-1	NM:i:2
+BS-DSFCONTROL03:121:D0M8KACXX:5:1210:6622:23752	147	chrX	7127	60	100M	=	6879	-348	CACAATGTCATCGAAACCACTCTCTTAAAACTGCAAATTTCTGGATTTTCGACCTTAAAATTTTTAAAAATTCCCAAAAAATCAAATTTCTGTCAAAATT	CEDDDDDDDDDDDDDCDCCDEEEEDEFFFDFFFHHHHHHJJJJJJIJIIHGIJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:118:7183:17666/1	16	chrX	7624	60	101M	*	0	0	AAACTTTCTTGGGTTATGAGTCTCAACGTATCCACGTGCATGACGGAAGAATTGGCTCAAATGCTCGCGAAGCTGCTCGGCAGACGTGTAAACGTCCAAAA	9=:?7@?C@CACA?ECCC=CCC:B?5B@BA:A=CCEACDDBEEFFGFBBGGEGGDDGGGGF=EGFGFFGGGGGGEGGBEGGGGGGGGGGDGGGGFGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+EAGLE:2:55:11225:9062/1	16	chrX	7903	60	101M	*	0	0	CAAAAAATGAAAATTTGGGCATAGCTTCCGCATCTCCAGTGACAAATGTGCTCTATTGCACAAATTGTTGGTTGGAATACGGTATTTATTGCATGTTTCAG	EGEEEEEEEEEADBBDGGFEEEDBEBCEFDGEEGEGGGFAFEEGGGFGGGEGFGGEFGGEGFEEFGFGGGGAGGGGGFGGGGFGGGEGGFGGGDGFDGFGG	PG:Z:SNAP	RG:Z:266-1	NM:i:3
+EAGLE:2:78:6338:2856/1	0	chrX	7939	60	101M	*	0	0	CGGTGACAAATGTGCTCTATTGCACAAATTGTTGGTTGGAATACGGTATTTATTGCATATTTCAGCCGAGAATACCACTATTCAAACCATCAATTTGGATA	DDD@DEEEEEGBDEGFGGFGGGGEGGFGEGGEBFEBEFFADDEFFFEEEEEDEEEEEDEEEEGBEEEEEEEEEEEEEEAEEEEEEEEE@EEEEAECCCABE	PG:Z:SNAP	RG:Z:266-1	NM:i:2
+BS-DSFCONTROL03:121:D0M8KACXX:5:1115:15278:33162	73	chrX	7942	60	100M	=	7942	0	TGACAAGTGTGCTCTATTGCACAAATTGTTGGTTGGAATACGGTATTTATTGCATATTTCAGCCGAGAATACCACTATTCAAACCATCAATTCGGATAGA	@@@FDDDDFDDDAGFHJJGIDHICFHEFCBFGGBDA@BDDGEH:?GA>GEFG<?DGHDFGGGGEB<BHHHEEC>DCDEEEFDEE??>CA@A@C@B@DBA>	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:1115:15278:33162	133	chrX	7942	0	*	=	7942	0	TAATAAGCCACTTTTCAAATAATCGCTGAATTCTAAGTAAAACATGTTTATTTTCAGTATATTTATCAGATTTAAAAAAAAAAGTGAGATTGTGTTAGTT	##########A:3CC;<DC?A?B@CCDCDCC@;C>AB;)7)))3=.7.))=D=.))));=;=)8B<FB89FB(GDHFA@HCA2E4?A+2+3FA+2A+1+1	PG:Z:SNAP	RG:Z:000
+EAGLE:1:50:11162:12440/1	16	chrX	7960	60	101M	*	0	0	GCACAAATTGTCGGTTGGAATACGGTATTTATTGAATATTTCAGCCGAGAATACCACTATTCAAACCATCAATTCGGATAGAGAAACACACAAGTCACAGA	FAEEGGDGFFEGGGGGGGGGGEGGDGDGGFGGGFGEGFGGGEFFAFGGGGFGEGFEDFFFCBGGEFGFGEGEGGFGGGGGGGGGGGFGFGGGGGEGGGEGE	PG:Z:SNAP	RG:Z:266-1	NM:i:2
+EAGLE:2:103:14619:17650/1	0	chrX	8476	60	101M	*	0	0	ATTGTTTAATTTTTCCGGAAAAAAACTGAAAAATCCAAAATTTTTGAATTTTCTTCATAAAAAGTCGGGAAAACCCAGAAATTTTCCAATTTTTTCCGGAA	GGGGGGGGBGGGGEGGGEGGFGGFGGGGDGGGGFGGGGEGGFGGGFGGGGGGGGGGGGG?FACEDGGEGDDGBGGGFFFGCFEGGGGGBGGGGGGE?DGEG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:5:12119:5229/1	0	chrX	8820	60	101M	*	0	0	TTTCTGAAATCCATAATTTTTCCAATTTTTTTGGGAAAAAATCCAGTAAAAGCCAGTTTTTCGCAAATTTCCATCCAATTTTCACCCTTAACGGCACAACA	EG?EGGAFGGGDGDDAGGGDFFFEGGGGFGGEGEEGEGGEGDEGGGFGDFFFFDGGFGAGFDG?GEB?FFGFGGGDDEGEEAFDF5DECE:CE=E=CCECD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:73:11201:4957/1	0	chrX	10521	60	1D6M1I94M	*	0	0	GTTTTCCGGGAAAACTGAAAATTTGGTCATAGCTTCCGCATCTCCGAGTGACAAATGTGCTCTATTGTCAAATTGTTGGCAGAAATGCGGTATTTATTCAC	GGGGEGGFGEBCECEGGDGGGGDGGGGGGDGGEFGEGGGDGEGGGFFFFFDFFFFGEGFFFGFGGGGEFGBC?DDFFGDFFFAFFEEAEE?ADE=DEEEDE	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:46:5340:5404/1	0	chrX	10534	60	101M	*	0	0	AAAGAAAATTTGGGCATAGCTTCCGCATGTCCGAGTGACAAATGTGCTCTATTGTCAAATTGTTGCCAGAAATACGGTATTTATTCACATTTTCAGCCCAA	GGFGGDFGDG?F=FFGEEGGEGGGGBCDGEGGEEFEFCFDEEEEBGFGEGGFEFEGGBEFFF@DEFFFDD??BBC???@?EEEEEBBBBBEEEE?EEEEEB	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:2:10:12798:8231/1	0	chrX	10540	60	90M1D11M	*	0	0	AATTTGGGCATAGCTTCCGCATCTCCGAGTGACAAATGTGCTCTGTTGTCAAATTGTTGGCAGAAATACGGTATTTATTCACATTTTAAGCCAATTTAAGG	GGGGGFFFFFGGGGGGGGGGGGGGGGGFGEEGGGGFGGEGGFGGGGGGGGGGGGGGGGGGGGGEE?EEEEGEGGGGGGFGGGDGGEDGBAEGGEFDGEAGG	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:113:16640:19734/1	0	chrX	10540	60	90M1D11M	*	0	0	ATTTTGGGCATAGCTTCCGCATCTCCGAGTGACAAATGTGCTCCATTGCCAAATTGTTGGCAGAAATACGGTATTTATTCCCATTTTCAGCCAATTTAAGG	FGGGGGGFGAFDFFFGGGGGDGGGGGGGGDFEEDBFFFEFGGGGFGGFGGBBGGGGGGGGFBGEEGFFECF??EECCEECEFEEEEE>CB:@BAACEEDEF	PG:Z:SNAP	RG:Z:266-1	NM:i:6
+EAGLE:1:75:12666:4476/1	0	chrX	10543	60	87M1D12M1I1M	*	0	0	TTGGGCATAGCTTCCGCATGTCCGAGTGACAAATGTGCTCTATTGTCAAATTGTTGGCAGAAATACGGTATTTATTCACATTTTCAGCCAATTTAAGGCTG	GGGFDGFFFGGGGGGGGGGFDGGGGGBGGGFFFFFFFDGGGGGGFGGFGFGEDGGAEGGGDGGGGGFFEEFFFEDGGEGGGGGGGDFGGEBDFG=EEFFCB	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:56:15981:20326/1	0	chrX	10549	60	81M1D20M	*	0	0	ATAGGTTCCGCATCTCCGAGTGACAAATGTGCTCTATTGTCAAATTGTTGGCAGAAATACGGTATTTATTCACATTTTCAGCCAATTTAAGGCTGAAAATG	FFFFFEGGGEFGGGGGGGCEBGGGGGDFEDGFFEGFGFGEEEEEGGGEEEBFAEADDBDBEE>EEEEBEE?:???ABA??AAA@AABB?B88A>?BBBB<A	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:26:17260:3333/1	16	chrX	10550	60	101M	*	0	0	TAGCTTCCGCATCTCCGAGTGACAAATGTGCTCTATTGTCAAATTGTTGGCAGAAATACGGTATTTATTCACATTTTCAGGCCAATTTAAGACTGAAAATG	BB=BB?=B=BB:B=BDCBB:B?BABBCD?BDBAC=BBDCAAA:AD:DCBDBB@?BDA>C:C;C?@;:C?:@CCAC?CA>AA=5BB;:5A?:D=?DDDD5?D	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:2213:8832:90154	99	chrX	11838	60	100M	=	12309	571	GTCTCCAGCTTCAAAATGACCCCCATCATGCCCTGTTTCCGTGCAATTTGAAAAATTCGGGTCTCGCCACGCGTTTTTTTTTGCAATTTTCGAACGAACT	CCCFFFFFHHGHHJJIJJJJJJJJJJJIIIIJJIJFHIJJIIIIHIIIJJBGGGJJJJFIIEHGEFHFFFDDDD6;@BDBDDDDECDDDDCBBBDDDDBA	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2213:8832:90154	147	chrX	12309	60	100M	=	11838	-571	TCAAAAATATTGAAAAGCCACCGATAGAGAGGATTTAGTGTGAAGAAAAAGGGGTGAAAAAACACCGGAATTGGGAGAGATATAGGACACGTCCATATCT	05CDEECD@C:5C>??@BD?@DCCEEEEDDFFCDFHHHHHED@7)JJJIIJJIJIJJIHGIGHGIHGJIJIJJJIGIIIJIGIJIIJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:1101:20052:62684	99	chrX	12814	60	94M6S	=	12857	139	CTGTATAACAGTGTGTAATTTCAAAAAAATCGGACGCACACACGCTGACTTGTGAGGCTTCAAAAAAAAAGTTGAAATAGTCAAAAAAAAAATCCATCAT	CCCFFFFFHHHFHHHIHIJJJJJJJJJJJHGHJIJJJJJJJJIHIHHHFEFFFFDEECDDDDDDDDDDDB>:CCC@CACC:CCDDDDDDDDD<A######	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1101:20052:62684	147	chrX	12857	60	4S96M	=	12814	-139	CCACGCTGACTTGTGAGGCTTCAAAAAAAAAGTTGAAATAGTCAAAAAAAAAATCCATCATTTTTCTGCCCAAAAATTGAAAAAACGGGCACCTATGCAC	####CC@::<DCDCCCA>>:8-BDDDDDDDDCCA>DDC:C><=DDFHHGFG@IGGJJJJJJJGHEIGIIHBIJJJJIIIGJJIHGIIHHHFHFFFFDB@@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:61:3791:13082/1	0	chrX	13027	60	101M	*	0	0	AAAATATGAAAATGTGATAGAGTGAATCATTGCGATTCCAATGAGGTAAAAAACTTGGCAAAATTCGACAAAAATTTCATTTTGACACTAAACACTTTATT	GGGGGEFE?GEEDBEEEEEEFGFGGEFFEADGGGGE?GFFGGFGGGDGFGFFGFFFFBGGBFEEFGGGFGGDGEEBGEEEFFGDBEBGFEGFEEEGEEBGD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2313:12490:88378	99	chrX	13038	60	100M	=	13177	239	ATGTGATAGAGTGAATCATTGCGATTCCAATGAGGTAAAAAACTTGGCAAAATTCGACAAAAATTTCATTTTGACACTAAACACTTTATTCAAAACACTA	CCCDFFFFHHHHHJJJJIJJJIJJJJJJJJJJJJJHIJJJJJJJJJJJJJJJJJI7FHJJJIJJHHHHHHHFFFFFFEEEEEDDDDDDDDEEEDDDDDDD	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2313:12490:88378	147	chrX	13177	60	100M	=	13038	-239	TATTTCTCAAAAAAGATTATCTTAAAATGTTGAAAATGATTATCTTAAAGATTATCTTAAAATGTTGAAAAAAAAAACACCGACTACCGTAACCCAGAAC	@DCCBB?BDDDDDDDDDDDCDCDDDDCDDDDEEFDCCCDDDDDEEDEEECEFFFEFFHHHHEAGIGGJJJJJJJJGGGFJIIIIHGJHHHHFFFFFFBBB	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:84:6328:7727/1	0	chrX	15454	60	49M1I37M2I12M	*	0	0	CAGTAACCTGACGCTGAATTTCGCGGCGAGACCCAAAAATTCCGAAAAAACCATCAAAAACAATCAAAATCTGCCAAGAAGGAAGGGGGTTTGATTTAAGT	GFGFGGGGGGEGGGGGGFDFGGGGBGGFFBEGEGBGGGFBBGFEDGFGBG?GGEFFGGGFGEE7EBDEFDDDECFEECEE@EACCCCCC6:AA@:AC?C@;	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+BS-DSFCONTROL03:121:D0M8KACXX:5:1205:12522:62956	99	chrX	21169	60	100M	=	21409	340	GTATAATCATGACTAGGTTCCGGTAATTTCGGAAAATATTGGGTCCCGAAGCGAAATTCCCCGGGTTACTGTAGGCCTTCGCGGCGAGACCCAAAAATTT	@?@FFFFFHHHGHJIJJCFIIJFIJIGIJIIGDGGIFIIJGIJDFHIIBG>HEDGHFHFFFFDDBBBDDDCCC>C>>CAC@BB9999>09@ABBDDA<:>	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1205:12522:62956	147	chrX	21409	60	100M	=	21169	-340	ACCTAAAATTTCGCGAGAAATTCAATTTTCAAGCGAAAAATTTTAATGTCCGAACGCAGATCGTCGAGAGCTTTCATTTAAGTATAACCATGCCTTAGTT	CCC@DC@B>@@BB?<ECCA@>>CCCADDDDDDBEEFCEAEECDJIIGHGGIHF?GEGJIIEIHD<EBGFGIIIHIJ@GGIJIIIGBAGGHHGFFFFFCB@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2112:18264:40909	99	chrX	23955	60	100M	=	24075	220	ACCGTAACCCGAAATGTTCAAAATTTCGCCGCGCCGGGGGTGAAAAATGACGGAAAAATTGAATTTCGCACTACTTTTTGCGTATTTCGATTGGATTTCG	CCCFFFFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJHFD=@DDDDDDDDCBDDDDDDDDDDDDEDDDDDDDDDDDEDDDDDDBDDEEDDDDDDACDDDA	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2112:18264:40909	147	chrX	24075	60	100M	=	23955	-220	TTCTTAATTGGCGGATATTCGACTTTGAGCGGATTTGAGGTTCTAACAATATCAGAGTCGGCGAGCGACAGCCGCGAGAAGTTGTTCCAGTCTTTGTGCA	CCACDDDDDDDDCCEDDDDDCADDCDDDDDDDDDDCDDDDDDDDDEEFEEECEEDFFEGJJIJIJIIIJJJJJJIJJJJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:62:3773:4884/1	0	chrX	24873	60	101M	*	0	0	CCCGAAAATTTGAAAATGTCCGCTAGAGACCCGGTAAAAAGACGTTCTAATGTGAAATTTGGCGAGGAATTCGAATTTCATATTAAAATATGTTAACATCA	GGGGGGGGGG?FFEFEGGFGGGGFGF?DFFFFFFCGGGFGGEGFEAEEBEDFCEBEEDDE5BEECCEACCCDECAEEEEEECDEEBEDCCACACCEEE@EE	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:2:111:17901:21150/1	16	MtDNA	148	60	101M	*	0	0	TAGGATTATTAGATATATTAATATTGACCCTATAAAAAGAAGTTTTTTTCTTATCTTTTCACTACTTTTTAGTATACCAGTTATTTCAATGAGAATACATA	CB???CA=AC=BBCADBDC?B?B?BA5DCAA8C?ADDABDC?CAACDDDDBDDDDDDADDBD=5DDDDBDDCDDDBDDDDDDDBDD=DDBD:DDDB>AACC	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:24:11474:6931/1	16	MtDNA	422	60	101M	*	0	0	GGTTTAAGAGGTTTTTATTATAGTATTTACTGGTTTATTTTTTGTTTTATTTTAGTATGTTTATTATTTTTTATAAATTTTAGTAGTTATTTTTTACATTT	DCCCA5:DDBC>BCD5D:DD=:D6DDDDBDD?CCCCACCCCCDC<CC>B=DDDBDDCDDDDBCCCC<CBDBDADDDD=::D=EED@CBBBE:DBDD5CC@A	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:1215:12199:50114	99	MtDNA	542	60	100M	=	651	209	GTTTAAAATTATGTTTTTATTTGTTAGATTATTTATATTTATTTTTAAATGACAACGTTTAATTTTTATTCTAATTTCTTTAGAATTTATAATGTTGAGA	@@CFFFFFHHHHHJJJJJJJJJJJJJJGIJJJJJJJIIJJIIJJJJJIIJJIIIHIIIJJJIIJIJIJJJHJJIJHHGIJJJIDFHHGFHFDEFFFFEC=	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:37:4190:5792/1	0	MtDNA	597	60	101M	*	0	0	CGTTTAATTTTTATTCTAATTTCTTTAGAATTTATAATGTTGAGATTATTTTTAAAATTTTCTTATGTTTTAGGGGAAATAATGTTTTTTTATTTTATGTG	GDEGGGGGFGGGEFGGFGFGGFGGGEGEGGGGGGGDFGGFGGEFGGGEFGGGGGGGGGGEGGGEEFGEGGGGGGGGEEEGEDGG?EEEAAE7EEEC?EFAA	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1215:12199:50114	147	MtDNA	651	60	100M	=	542	-209	AAATTTTCTTATGTTTTAGGGGAAATAATGTTTTTTTATTTTATGTGTTTTTCTGTTATTTCAAGAATCCTGGGTATGGTAGTTATAGTAGGTAATATAA	ADCCDDDCDDDDDDDDDDDDEEDFDEFFFFHJJJIJJIJJJJIJJJJIIHJIIIJIIGIGDIJJIJIGIJJJJJJIJJJJIJJJIJJHHHHFFDDFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:51:1652:7346/1	16	MtDNA	726	60	101M	*	0	0	ATGGTAGTTATAGTAGGTAATATAAAATTTTTTGGTAGTGATAATTGTATTTTTTAGTAACAGATATAAGTTAAGTTTAAACTATTGATCTTCAAAATCAA	BEEFEFEEEEBEFBFEEEAFEFFEFFAFFF?EFFFFFFEFFFFFFFFFFAFFFFBFFEFFEFFFBFFEFFEDEDFDFAFFFFFFFFF?FFEFFD?FEFFFF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2207:3477:80131	99	MtDNA	800	60	100M	=	937	237	GTTTAAACTATTGATCTTCAAAATCAAAAATTTATTTCTGTAGAGATAATAGTATAAATAAGTATGTTTCTTTTTCGCAGAAATGGTTTTTTATCTTATA	CBCFFFFFHHHHHJJJJJJJJJIIJJJJJIJJJIJJJJJJGIJJJJJJJJJJIJJJJJJJJJHFHIGIJJIJJJJJIIIIIHHHHHFFFFDDDDDDDDCD	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:77:16898:20473/1	0	MtDNA	800	60	101M	*	0	0	GTTTAAACTATTGATCTTCAAAATCAAAAATTTATTTCTGTAGAGATAATAGTATAAATAAGTATGTTTCTTTTTCGCAGAAATGGTTTTTTATCTTATAA	ECEE?FFFFFGGGGGGGGGGFGGGGGGGGGGGGFGGGFGGFFGEFEGFEGEGFDGEEFGEEGEEEEEEFEFGEFEEEEBG?ACDEFBDDEEDCB=FEEFEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2207:3477:80131	147	MtDNA	937	60	100M	=	800	-237	TAAGATGATTTAAAATGTTATTATCTAAATTTGATTTACAGAGTAGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAAT	DDDDDCCECDFEEDEEEEEEFFFFFFFHHHHHHJJJJJJJJJJJIIIHIGJJJJJJIHF?IJJJJJIJJJJJJJJIJJJJJJJJIGCHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:1
+EAGLE:2:46:4245:14190/1	16	MtDNA	952	60	101M	*	0	0	TGGTATTATCTAAATTTGATTTACAGAGTAGGCAATAAAAATTTACCTCGGCAATTTATCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACTTGT	CGBEEDEDEFEGEGEGEDDGFEEBDGGGDBGEE@EFGGGGGGFECEGGGGEBFFGGFGFGGGGGGFGGDGGEFFFFEBGGFGGFFEDFGGGFGFGEFGGGF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:27:7941:4769/1	16	MtDNA	1010	60	101M	*	0	0	TCGCTTGTAAAATACTTGTTCCAGAATAATCGGCTAGACTTGTTAAAGCTTGTACTTTAATTGATGTTAATTATGAAATTATTATATTTTCTTTTAGATCT	CG?BGGGAFGFDDCFBGGGFFEGGGGDGGEFGEGFGGGGGGGFGFGGBEGGFFDADGGEGGGGGGDGGGGGGGGGFGGGFGGGGFGGGGFGGGGGFGGGFG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:84:2306:5557/1	16	MtDNA	1132	60	101M	*	0	0	TTAGTGAATTTTCATAATTTTAAGATTTGTTGAACAAAGCAGATTAGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAAACTGAA	CCE?EEACBBEBFFFBEEE5EABF?FFEFFFEDAE=EFFECEDEEDDD@DDEEEDDDECFFFFAFFDEBE?DFFDFFFFFDDEEDA=EEEED:DDDEEBEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:111:11302:7630/1	16	MtDNA	1137	60	101M	*	0	0	GAATTTTCATAATTTTAAGATTTGTTGAACAAAGCAGATTAGTACCTGGTTAGACAAAAATTAAAAGAGCAGGAGTAAAGTTGTATTTAAACTGAAAAGAT	AEEAEE:EAECD?BB?DGGGAGDFAGGDDGGGGGGGFF:FFGGDGGEEEDEAGGEGGGFGDGEFDGGAFGFFGFGGDEDGGGGDDGGAG=GGGGFGEGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1205:2947:30776	99	MtDNA	1208	60	100M	=	1331	223	GGAGTAAAGTTGTATTTAAACTGAAAAGATATTGGCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTT	BCCFDDFFHHHHHJJJJJJJJJJJJJJJJJIIJJJJJJJJJJJIIIJJIJJJJJJJJJJJJJJJIJFFHHIIJJIHHIJHHHHFFFFFDEEDDEDEDEEE	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:43:15776:12730/1	16	MtDNA	1242	60	101M	*	0	0	GCAGACATTCTAAATTATCTTTGGAGGCTGAGTAGTAACTGAGAACCCTCATTAACTACTTAATTTTTTGACTCGTGTATGATCGTTTATTTTATTCTTAA	EDDC:EDDCEBGEEEEDEEEEEGFGEEEGGGEFGGGGDGFGFFACEAGGDFFFECFGCGEGCEGGFEF?GDGGGFGGGGGGFFGGFGDFGGGGGEGGGDGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1205:2947:30776	147	MtDNA	1331	60	100M	=	1208	-223	TTTTATTCTTAAGGATTATAATAAAAAATTTTTAATTTATTAAAATAGATATATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATAT	@DDDDCDACDDEDEFEEEFFFFFHHJJJIJJJIJJJIIJJJJJJJJIIHDJIGD?JJIJJJJJJJJGJJJJJJJJJJJJJJJJJJIHHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1208:16741:52367	99	MtDNA	1375	60	100M	=	1446	171	ATAGATATATACCCGGTTTATGATTTAAGAAACATTTGGCCTACAATATTTTATATTATGGATTTTAGTTTTAGTTAACTAAATGAAATTGTAAAAGACA	CCCFFFFFHHHHHJIICHHJJJIJJJIIGIJJICHJJIJGJGIJJJJEHIJIHIJJIIIIIJJIIIJJFHIJJIDCEEEHHHHFFFFFFEEEEECECBDC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:51:9770:4736/1	0	MtDNA	1428	60	101M	*	0	0	TATTATGGATTTTAGTTTTAGTTAACTAAATGAAATTGTAAAAGACAGTAAAAAATTCTTAATGTATTTTTGAAGATTATCTAGAAGTGGTACAAATCATC	FFEEFDFDFFFFFDFBD5DDEEEEEEEE=EEEEEEEEE?EEEEEEEEEEEEEEEBCEEEDEEDEEEEEEEEDEABEDDEEEEEDEEEBEEDEEEEEAEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1208:16741:52367	147	MtDNA	1446	60	100M	=	1375	-171	TAGTTAACTAAATGAAATTGTAAAAGACAGTAAAAAATTCTTAATGTATTTTTGAAGATTATCTAGAAGTGGTACAAATCATCCATCAATTGCCCAAAGG	DDDEEECEEEFEFFFFFFFHHH@FFHJJJJJJJIIJJJJIJJJJJJJIIIJJIJJJJJJJIJJIGIJJJJJIGGJIJJJJIIJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2306:21063:70183	99	MtDNA	1578	60	96M4S	=	1595	108	ACCTGAATCTAGTAATAAAACTATTTTTAAATATGTTTTGAAAACATGTTTTGAGGTAACTCGTAGTTTTTAAGAGTTAGTTTAATATAGAATTGTTGAT	@CCFFFFFGHFGBGGGHJGFHIHGIJJICGHIEIHGIJGIFDDDGHIG@D?FEHHIHHHHIJGGB@CF@GC@DHE3.?EHHHCF7@37.6>6>>;@####	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2306:21063:70183	147	MtDNA	1595	60	9S91M	=	1578	-108	CTAGTAATAAAACTATTTTTAAATATGTTTTGAAAACATGTTTTGAGGTAACTCGTAGTTTTTAAGAGTTAGGTTAATATAGAATTGTTGACTGTTAATC	#########@B>67;3@;B?<HC;==JJIHGGFFHF=89B@GGHFB8DBB?8)?*C<>JIIIGFC9A2?C<++JJJJIIEIHFC;EJHHHHHFFEDDBB@	PG:Z:SNAP	RG:Z:000	NM:i:1
+EAGLE:2:62:8987:8931/1	0	MtDNA	1620	60	101M	*	0	0	AACATGTTTTGAGGTAACTCGTAGTTTTTAAGAGTTAGTTTAATATAGAATTGTTGACTGTTAATCAAAAGGTGTACCTCTTAATATAAGAGTTTAGTTTA	GGGGGGGGGGGGGGFGGGGGGGGGEFGGGGEGDGEFFFDFGGGGGGGGGGGGGEGFEGGGEFDFGGFFFEFFAFECEEEFEGEEGEGEEGBFBEEEDCDEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:58:15307:9170/1	0	MtDNA	1657	60	101M	*	0	0	GTTTAATATAGAATTGTTGACTGTTAATCAAAAGGTGTACCTCTTAATATAAGAGTTTAGTTTAAGTTAAAACGTTAGATTGTAAATCTAAAGATTATTGC	DBDD:CDCBCEBAEEEC=EEEBCBBB=BBB@>>CC5C>AA?>>;>@@FBD@@E:B@@@BB@@5B?B>BBBAAA:A@BB@@@D@@@@@A?BA?@>@@@@@@?	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:87:9699:18394/1	16	MtDNA	1707	60	101M	*	0	0	AAGAGTTTAGTTTAAGTTAAAACGTTAGATTGTAAATCTAAAGATTATTGCTCTTGATAATTTTAGTTTTACTTATAGTTATTTTAATGATGATTTTTATT	BEFGEBEEGEEEEEEGBEGGEEFGEGGGGGGGGGDGFEGEGGGGGFDGGGGGGGDGGBGGBGGGGGFGGGEGGGEGGGGGGGGFGGGGGGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:100:3634:12538/1	16	MtDNA	1809	60	101M	*	0	0	TTCAAAGAATCGCTTTTATTACTCTATATGAGGGTCATTTATTGGGAAGAAGACAAAATCGTCTAGGGCCCACCAAGGTTACATTTATGGGATTAGCACAA	EA=EE??ED:EA<?:A=EED5EEE=E<<>B>8;;9?EE?EEEEEDDDEAEEEBEDEEEEEEEEEEEEEEEEEEEEEEEEEEEEEA?FGGGGDDGEGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:1111:21372:42529	99	MtDNA	1832	60	100M	=	1878	-100	CTATATGAGCGTCATTTATTGGGAAGAAGACAAAATCGTCTAGGGCCCACCAAGGTTACATTTATGGGATTAGCACAAGCTTTATTGGATGGGGTTAAAC	@B@FFFFFHHHHHJIIJFHIIJJHGIIHHGJJJJJIIHGHIIJJJBFHJIFGGFHEIHIIIHHACHHFEFFFEEEECEBBCDDCDDDCCDDD@DBB?CAC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1111:21372:42529	147	MtDNA	1878	60	46S53M1S	=	1832	-100	NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCACCAANGTTACATTTATGGGATTAGCACAAGCTTTATTGGATGGGGTTAAAN	##############################################IHGFF?0#IIGEIIJIIJJJJJIIIJJJIIJJJIHHHHIJJHHGHHFDFDD=1#	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:1109:13191:17203	99	MtDNA	1898	60	100M	=	1981	183	GGATTAGCACAAGCTTTATTGGATGGGGTTAAACTTTTAAAAAAAGAACAAATAACACCCTTAAATTCCTCTGAAGTATCATTTTTACTTGTACCAGGAA	BCCFFFFFHHHHHJJJJIJJJJJJJJJJFHIJJJIIIJIIIJJIJJJJJJGIIJJJJJHHHFFFFFFEEEEEDEDDCDFEEEEEEDBDDDDDCEDDDDD<	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:73:15682:20928/1	0	MtDNA	1914	60	101M	*	0	0	TATTGGATGGGGTTAAACTTTTAAAAAAAGAACAAATAACACCCTTAAATTCCTCTGAAGTATCATTTTTACTTGTACCAGGAATTTCTTTTGTTGTAATA	GGGGGGGGGGGGEGGGGDGGGGFGGGEGEGEGGEGGFGFGGGGGBE?GFFEEGEGEECEC=CCCCEEEEECC@D?ACEC:CDACCEEEEEEE;EE6<AA??	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2108:8075:44880	99	MtDNA	1923	60	100M	=	2060	237	GGGTTAAACTTTTAAAAAAAGAACAAATAACACCCTTAAATTCCTCTGAAGTATCATTTTTACTTGTACCAGGAATTTCTTTTGTTGTAATATATTTAGA	@@@DDDFFFHHFHGIIJJIJIGGIJJJGIIIIIDIIIDGEHIGEGGIECHHFHACFHIIIIJGIFFCEEHEFFE>;ACECCCACBCBDDDDDFDDEDDDA	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:64:1358:10984/1	0	MtDNA	1931	60	101M	*	0	0	CTTTTAAAAAAAGAACAAATAACACCCTTAAATTCCTCTGAAGTATCATTTTTACTTGTACCAGGAATTTCTTTTGTTGTAATATATTTAGAATGATTTAC	GGGGGGGGEGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGEGFGGGGGGGFGGGGEEGGEGGEEEGEGGEGGAEF@D@CDDE@FDFEDDDDE@EEC?@	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2301:3386:87325	99	MtDNA	1965	60	100M	=	2135	270	CCTCTGAAGTATCATTTTTACTTGTACCAGGAATTTCTTTTGTTGTAATATATTTAGAATGATTTACGTTACCATATTTTTTTGATTTTATTAGTTTTGA	CCCFFFFFHFHHHIJJJJJJJJJJIIJJJJJJJJJJJIJJJJIJJIJJJJJJJJJJJJIJJIJJJJJJJJJJJJJIJIJIIHDDBECEDECDCDCDDDD<	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1109:13191:17203	147	MtDNA	1981	60	100M	=	1898	-183	TTTACTTGTACCAGGAATTTCTTTTGTTGTAATATATTTAGAATGATTTACGTTACCATATTTTTTTGATTTTATTAGTTTTGAGTATTCAGTTTTATTT	DDCCDDDDDCDDDDDCEEDDEFFDBHFHEHHHJJJJJJJIJJJJIHFGHFIIIHGJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:118:10385:6513/1	16	MtDNA	2048	60	101M	*	0	0	GATTTTATTAGTTTTGAGTATTCAGTTTTATTTTTTTTATGTTTAATTGGATTTTCTGTTTATACAACTTTAATTAGCGGTATCGTAAGAAAATCAAAATA	DEEFGGFGABGGEEFGEGGFGEGGGGGGGEGEAGGEFGGGGGDGGAGGGGGGGGGGGGGGGGFGGGGGGGGGGGDGGGGGGGGGGGGGGGGGGGGGGGGFG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2108:8075:44880	147	MtDNA	2060	60	100M	=	1923	-237	TTTGAGTATTCAGTTTTATTTTTTTTATGTTTAATTGGATTTTCTGTTTATACAACTTTAATTAGCGGTATCGTAAGAAAATCAAAATATGGTATAATTG	>>CCDCEDCC@CCDDDCDDBDEGHHHHHGCGGHIGHGGIIHEIGIGHGIFHHIGGHIGIIH@GIIHGGGGHJIIIJJIIHEA4IIIIFHHHHFFFFF@CC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2301:3386:87325	147	MtDNA	2135	60	100M	=	1965	-270	AGAAAATCAAAATATGGTATAATTGGGGCCATCCGTGCTAGAAGACAAAGAATTTCTTATGAAATTGCTTTTTCTTTATATGTTTTGTGTATTATTATTC	CCCEDDDDEEEDDCCEEEEECCBFFHHHHJJIIJJJIIJIJJJJIJJJJJJJJJJJJJJJJJJJIHGJJJJJJIJJJJJJJJJJJJJHHHHGFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:44:5556:17346/1	0	MtDNA	2177	60	101M	*	0	0	AGACAAAGAATTTCTTATGAAATTGCTTTTTCTTTATATGTTTTGTGTATTATTATTCATAATAATGTTTTTAATTTTGTTTCAAAATTTAATTTGAGACT	FFEGGGDFGGGGGGGGDGGGEGEGGGEDGFFGFGGFGFDGGGEGGEDFGGGGGG?GGGGEDEFGGGEGGGGGGGGGFFGEGGE?EGEFGGEEGGDFBBAGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:90:16871:20538/1	0	MtDNA	2198	60	101M	*	0	0	ATTGCTTTTTCTTTATATGTTTTGTGTATTATTATTCATAATAATGTTTTTAATTTTGTTTCAAAATTTAATTTGAGACTTTTAATTATTTACATCCCATT	FGGGGGEGGGGFGGGGGGGGGGGGGGGGGGGGG?GFGGGGGGFGGGGGGGGFGGGGF?GGGGDGDGGGEGGEFGGEGEGGGGGACGGGGFGDGGGFGFGFG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:1:15979:20440/1	0	MtDNA	2341	60	101M	*	0	0	TTTTTCTGAAGGTGAAAGGGAGTTAGTTAGAGGATTTAATGTGGAGTTTGCCAGAGTAGCTTTTGTTTTATTATTTTTAAGGGAATATGGAAGATTAATTT	GGGGGGGGEFGGEGEEFEEEFFFFFFFFDFFGGEGGGGDGGFGFEFEFFFGGDGDGCEGFGDGGGEEGGEGG=FGGEG?AGGG?DAEBEE=CFAEDDDDEG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2114:8192:26387	99	MtDNA	2360	60	100M	=	2687	427	GAGTTAGTTAGAGGATTTAATGTGGAGTTTGCCAGAGTAGCTTTTGTTTTATTATTTTTAAGGGAATATGGAAGATTAATTTTTTTTAGGGTACTTTCTT	B@BDFDEEHGHHHIFHIJIIIJBHHFHGFHIJJIIFHCBHGIJJJJGIJJHHJIJJJJJIEIJIEBGHIJJHHGEGHIEGGHGHDDDBCDD>CDDEEDC@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:86:11924:13707/1	0	MtDNA	2394	60	101M	*	0	0	GAGTAGCTTTTGTTTTATTATTTTTAAGGGAATATGGAAGATTAATTTTTTTTAGGGTACTTTCTTCTGCTATATTTTTTAAATTTTCAATTTTTATAGCA	GGFGGGGFGEFGDFGGFGGEGGGGGGFGGGEDFFGFDDEFGGGFFGGGGGGGGEGDFAFFFGGGGGGEGGEDFDDGGGGGEEEEGFGGEEEGEFGDC:=F5	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:23:12390:3932/1	16	MtDNA	2447	60	101M	*	0	0	AGGGTACTTTCTTCTGCTATATTTTTTAAATTTTCAATTTTTATAGCATTTAGTATTTTTTCATTATTAATTTTTATTCGTAGTTCATACCCTCGTTATCG	@A?A?:EE:?E==FGBDGEEGFFFB?DFBDEGG:FD>EGGBFGGFEFFGGEG@GEEFFE:BGFDFG?GDFGEGGGEEFFFEEF?FFEE@BDDFDFFDFDFF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:69:4343:18981/1	0	MtDNA	2510	60	101M	*	0	0	TTATTAATTTTTATTCGTAGTTCATACCCTCGTTATCGTTATGATTTAATAATAAGTTTATTTTGATTTAAACTTTTACCAATCTCTTTAATTATATTGTG	EECCCDDEEEFFEEECDDADEEEEDFGGFFGGGGAGGGGFEEE:EEEEEEF?GDDGEGGGGGGDGBFGDFFEGGGFGBGCDDFDFGEDGGAGGAGBFEBE?	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:106:17241:4836/1	0	MtDNA	2631	60	101M	*	0	0	ATTATTAATCAAGTTTATTTTTTAGACATTTTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAA	GGGGGGEEFGGGGGGGDGGGGGGGEGGGGGGGGAFGGFGEGDGGGFFGGFFEFGGGGGGDGGFFGGGGGFDGG?ACEEFFGDGEDFDD=FBAEEEFBF?FF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:118:1597:14954/1	16	MtDNA	2633	60	101M	*	0	0	TATTAATCAAGTTTATTTTTTAGACATTTTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAAT	FGGGEEGGBECG?GEGGGGGFGGBGEGGBFGGGEFGGFFGEAEDAEBGDGGGEGFGGGGFGGGBGGEEGDFGAGGFGFGFDFGGGEGFGEGGGEFGDGAGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:30:9587:17311/1	0	MtDNA	2647	60	101M	*	0	0	ATTTTTTAGACATTTTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTA	GGGGGGFFEDGGEGEGFGGGGGGGG;EEEEFGGGGGGGEGGEGGEEGFGEGGGGEGDGDGCCEEBFDFFFGGGDGGGGGGFGGDGGGFGGFGGGGGGFDGC	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:90:16079:8007/1	16	MtDNA	2653	60	101M	*	0	0	TAGACATTTTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGA	GEEBEA5GFGGGGGAEGGGGGGGGGFGFDFGGGGCGDG>GEGGFGGAGGGGDFDGGGGGFGFFFFBFFFDFGGGGFGGGGFGGFGGG?GDGDDGGGGEGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:48:1800:14293/1	16	MtDNA	2661	60	101M	*	0	0	TTTATATTTGTTTTTGTTTTACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGAGTATTTAG	FFDFBAC?ACC>E5FFFFFFFEFACCACE?C@ADE=FEFFFFFDFFFDFFFFFFFFFDEBFFFFFB?FFDF?F=ADFFFFFEEAEEDEEEFEFABDGGDG=	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:83:16330:7076/1	16	MtDNA	2681	60	101M	*	0	0	ACAATTTTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGAGTATTTAGATATACAAATACTTTACCAT	BEE@@<AC??C>>?FD:FCFFBFEFDFFFDFFBFFFBFFFF=FFBFFFFFFFDFBFFDFFFD=D=:FFDEFFFDFF?FFFFE=EEDDDEEEC@?ACCCBD=	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2114:8192:26387	147	MtDNA	2687	60	100M	=	2360	-427	TTTGTTTTATTTTAAAGAAAGTATATTAAATACTTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGAGTATTTAGATATACAAATACTTTACCATTAAGG	ECDEEFFFFFFHHHHFGHJIIIGHEEIJIJHHJIIJJHGJJJJJJJIIJIIIJJJJIIJIJJJJJJJIFIIIIJJJIJHJJJJIHFJGHFFHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:23:17204:14243/1	0	MtDNA	2720	60	101M	*	0	0	TTTAGTGAAAAAATTTCTTAATAGGTTAGTAGGAGTATTTAGATATACAAATACTTTACCATTAAGGTCAGTAATTTCTATTTTTACTTTTATTGTTCTTT	;;;9;:===:C;CCA=?DDDDDDDD6>C>>@@??B:D=DDDDDDDDDDD=DDDDDDDDDDDDDDD=DADDDABBBDDD=5DCD?C<DD?:DDDCCB=DCDD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:42:2835:3423/1	0	MtDNA	2726	60	101M	*	0	0	GAAAAAATTTCTTAATAGGTTAGTAGGAGTATTTAGATATACAAATACTTTACCATTAAGGTCAGTAATTTCTATTTTTACTTTTATTGTTCTTTTAACTT	@C@@=AAACCEDDEED?A=?C?A>C>AAABCDDDD=CCCA:AA?CDDDDDDABDBDDB?DC=C@??ACDD:CA9ABDC=>CCCDD=D8>BBBBBDC5BBCD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:73:15087:6864/1	0	MtDNA	2787	60	101M	*	0	0	TCAGTAATTTCTATTTTTACTTTTATTGTTCTTTTAACTTGTTGTTTTGGAGGTTATTTTACTTACTCTTTTTGTCCTTGTGGAATGGTTGAATTTACTTT	==?;B;C>=;CC?CC?>:8CA=?C:;==CBD?DDCB?B?DDBDD@ABA>ABDDBBCDAD>>CDDCDCDDDCD@A>>CDDDCDA5BC=D5DDB:B5@=>CCD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:41:4283:1044/1	16	MtDNA	2804	60	101M	*	0	0	TACTTTTATTGTTCTTTTAACTTGTTGTTTTGGAGGTTATTTTACTTACTCTTTTTGTCCTTGTGGAATGGTTGAATTTACTTTTGTTTATGCTGCTGTAG	FBCCFDEEGEEAEGEEEEFEGEGGGFGFGGGGGFFFAGGGGFFCFFDEGGGGFGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGFFGGGFGFGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2212:11575:96550	99	MtDNA	2839	60	100M	=	2949	210	GTTATTTTACTTACTCTTTTTGTCCTTGTGGAATGGTTGAATTTACTTTTGTTTATGCTGCTGTAGCGTGATTAAGTACTTTGTTAACTTTTATTTCAAG	@@@DFFFFHHHHHGIGHIIJJJIIIJIIDHGHDHHGEGHIEIJJIGIIIJGHGGGIIIGEEHEDGGI<AAHHIIFHHFHFHG>DFD;7>CEECACDCDCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:83:6736:2885/1	0	MtDNA	2866	60	101M	*	0	0	GTGGAATGGTTGAATTTACTTTTGTTTATGCTGCTGTAGCGTGATTAAGTACTTTGTTAACTTTTATTTCAAGAGAAAAATTTTCAGTTTATATAAGAAAA	EEEEEFFFFDDGEGFGGGGGGGGFEEEEEGGEBGEGEEFGGEGEEEEEBBCEDEEEEEEEEEEGEEEEEEEEEEEGEBEEEEEEEEEEEEEEEEDEEEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:62:11372:10324/1	0	MtDNA	2896	60	101M	*	0	0	CTGCTGTAGCGTGATTAAGTACTTTGTTAACTTTTATTTCAAGAGAAAAATTTTCAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGA	F?FFBDB?=D@:@@@CC:>5:;A;;DC>DDDD:ADDA@>B=DA?D?DDC@>=?AD?DBCBBDDCDBCBDB>>DA=??5?B?C?@?@=>@CBBBBC=>@>>B	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:91:13282:2427/1	16	MtDNA	2915	60	101M	*	0	0	TACTTTGTTAACTTTTATTTCAAGAGAAAAATTTTCAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGAATGCTATTAATTGAAATCG	CEEEEEEEEEEEEEEEEBBEEEEEEEEEEEEGGEEGEGGEGEGF>EFGEGEEEEEFGFGEGEGGGDEGEGGGFEFGGGGGGGGGGFGGGGGGEEGFGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:53:2586:12076/1	0	MtDNA	2916	60	101M	*	0	0	ACTTTGTTAACTTTTATTTCAAGAGAAAAATTTTCAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGAATGCTATTAATTGAACTCGT	GGGGGFGGFBGGGGGGGGGGFGGGGFGGD8EEEFFGGGEGGGGGG?GGGFFGGGGGFGGGGG?GAGEGGEDAFGFFEGGGEEEBGEAEEEEEEDE=::?>C	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:2212:11575:96550	147	MtDNA	2949	60	100M	=	2839	-210	TCAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGAATGCTATTAATTGAAATCGTTAGAGAATTTTCTCGTCCACTTGCTTTAACAG	ACDDDDDDDDEEECDEFDFFFHHHHHHCJIJIJIJJIHG@GHCJJJIHGGCIGIJJIIGIIJIHHGGJJIIIDIGGIGGEIIGIHG@HGHHFFFFFF@C@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:54:12110:15974/1	0	MtDNA	2950	60	101M	*	0	0	CAGTTTATATAAGAAAACCAGGAGACACATATTTGAAAACTCTTAGAATGCTATTAATTGAAATCGTTAGAGAATTTTCTCGTCCACTTGCTTTAACAGTG	GFGGGGGGGGGGDGGGGGGEFGGGDFGFGGGGGFGGFEEGFFGGEGEEBGFEGFGDGGFGEGFGGEEEDEEEEAEEEFEEGEEEEEEEEEEEEEBCCADA?	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:80:9039:4187/1	16	MtDNA	3058	60	101M	*	0	0	CAGTTAATATTACTGTTGGTCATTTAGTTAGAATAATGCTTTATCAAGGATTAGAATTAAGAATAGGTGATCAGTATATTTGATTATCAATTTTAGCCATT	:@?A@<BB;BB@AAAABBBB><AAAA@AAAAEE<EEAAAAAAAAAAEEE?EBBBB?EBEEEEEEBEBAAAAA?EBAE@EEEECEEEEEEEECBCAB=<;>@	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:24:5066:4097/1	0	MtDNA	3060	60	101M	*	0	0	GTTAATATTACTGTTGGTCATTTAGTTAGAATAATGCTTTATCAAGGATTAGAATTAAGAATAGGTGATCAGTATATTTGATTATCAATTTTAGCCATTAT	DGGGGGGGEAFFFFFFFFFFEAEEEEF?FEFEBFFFFGBBDEEEBGGEE=FFDFFGGEGEFFEFFAFDFEBFAEEDGEFGEEBGEGGEGEGDEDEEEFBEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:105:7673:2329/1	16	MtDNA	3249	60	101M	*	0	0	GTTAACTTAAGTTTTAAAGTGCCAAACTTTTAATTTGGAAATGGTGGACCACATCTTAGTTGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGA	EE5DEE5FFEEFBAEFD@?==D5:C?F5FFFGGBGFFFDFF=EEEECBEEBDE:DD?:GGGDGGEGGGGGEGGGGDGGGGGFGGGGGGGGDGFFGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1113:17155:40031	99	MtDNA	3295	60	100M	=	3421	226	GACCACATCTTAGTTGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGT	@BCFFFFFHHHHHHIJJJJJJJJJJJJJIIIEHIJJJJJEHIJJJJJJJJJJJJJJJIJJJHIJJIIGHHGDFBCAEEEEEDDDDDACEEDDDEDDEDDE	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1305:16341:33179	99	MtDNA	3300	60	100M	=	3308	108	CATCTTAGTTGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGTTCTAG	CCCFFFFFHHHHHJJJJJJJJIJIJJH>HIIJJJHIIJEGJIJJJJJJJIIJJJIJIJIJJIJJJJHHADFFFFFDCECEECDDEDDDEDDEDDDDDEED	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1305:16341:33179	147	MtDNA	3308	60	100M	=	3300	-108	TTGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGTTCTAGGTTAAATC	CEEEEEDEDDDDDDCDDDC@EEEDFFFFFFHHIFJIJJJJJJJIJJJIHHJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFC@C	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:61:8761:13140/1	0	MtDNA	3309	60	101M	*	0	0	TGATATAGCATAAGAAGTGCATTTGTTTTAAGCGCAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGTTCTAGGTTAAATCCT	FFFFF?EGGGGD=FGGFFGGDFDFFGGGGDFDGGGD?BDFFGBGGGGDGFDFFFFGGBDDEFEFFGDEFEEGBGEBBC?BBBBBCADBEDCB:CA?@B@@:	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:72:9498:11197/1	16	MtDNA	3343	60	101M	*	0	0	CAAAAGATATCCGTCAACTAACGAGTTCATAAAGCAAGTCTTCTAAATTTGTTCTAGGTTAAATCCTGCTCGTTTTTGATTGTTTTTATTTCTTTATTTAC	EED=DF?>>A?A=>5@?CDBBFB=EFFEEDBBFDDFEDFFFFFDDFFD;FF?FEDFDFDFFEEEBDEFDFDDBFFFDFDFEFFFFFD>EFAB>EAADDDCB	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:30:13606:9054/1	16	MtDNA	3373	60	101M	*	0	0	AAAGCAAGTCTTCTAAATTTGTTCTAGGTTAAATCCTGCTCGTTTTTGATTGTTTTTATTTCTTTATTTACCTTGTTTTTAACATTATTAAGAATTTTGAC	EA5B??:EEEEEEEDEDE=DBBEEB=FBEDEEEEEFDEGGEEGGFEEGA5DEGEGGEAEGGECB=FEFF=FFGEGFGFFDGFFFFDEFGFDGGGGGFGDGF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1113:17155:40031	147	MtDNA	3421	60	100M	=	3295	-226	ATTGTTTTTATTTCTTTATTTACCTTGTTTTTAACATTATTAAGAATTTTGACTAATAACGTTATTGTTTGATGAAGAATTTTTTTATTGATAACTGTAG	CDDDDDDDDDDDDEEEEEEEDCFFFHHHHJIJJIJJJJJJJJJJJJJHGDIIJJIIJIHJJJJIJIJJJJJJJJJIGIIJJJJJJJJHHHHHDDFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:79:5551:14500/1	16	MtDNA	3442	60	101M	*	0	0	ACCTTGTTTTTAACATTATTAAGAATTTTGACTAATAACGTTATTGTTTGATGAAGAATTTTTTTATTGATAACTGTAGTTTTTATTCTATTAAATAAAAG	5DGEGGGGEGGGEGFGGFFFGFGGGGGGGGEFGFGGGEFGGFGGGGEDGGFGGGFGDEGGGEGEGFGGGGGFAGGGGGGGGGGGGGCGGEGCGGGGGGGGF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:118:9818:14692/1	0	MtDNA	3448	60	101M	*	0	0	TTTTTAACATTATTAAGAATTTTGACTAATAACGTTATTGTTTGATGAAGAATTTTTTTATTGATAACTGTAGTTTTTATTCTATTAAATAAAAGCAGCAA	GGEGGEGGGEFGGGBGGFGGGGGGGGFGBFEGGGGFFGGGGGGEGGGGGGEGEGADGGGEGFEGGGGGBGEFFEFGGFGGGGEGGGGGG?GFGAGEGGGCB	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:50:8060:11985/1	0	MtDNA	3466	60	101M	*	0	0	ATTTTGACTAATAACGTTATTGTTTGATGAAGAATTTTTTTATTGATAACTGTAGTTTTTATTCTATTAAATAAAAGCAGCAAGAGATATACCAGAATTTT	EEAEAC=D=D:AACA@;@=@>@<@@DDDD?CA5C?DDDDDCABCADD=DDDDDDDA=ADA?>><DCCD:DD:DD>AADDACCCCBBCAD?CBCDC?B:=BD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:56:13819:18497/1	0	MtDNA	3542	60	101M	*	0	0	GCAGCAAGAGATATACCAGAATTTTTAATTATTTTGTTATTCAAGAGTCTTTAGGTTTATTATTTCTTCTTTGTAGAGGAGGTCTATTACAATTTTTTATT	GGGGGGFGGGGGFGGGGGGEGGGGGGGGGGGGGGGGGGGGGGGGGEGFGGGGGGGEGGGGGGGGGGGGGGGGGFGGBEGEGEEGEEGFEFEEEFEGEEFEA	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:69:5893:18114/1	0	MtDNA	3759	60	101M	*	0	0	TATTTTATTACAAATTTTTTGGTTAAGATCCGTGTATATTTTGTTATTTGGTTTATTGATTTGTTATGTTCAAATTTTTGTCATAAAAAGTTATAAAAATT	FGGGGGGGGGGGFGGGGGGGGGGGGFGGGGGGGGDAGGGGFFFEEAGGGDGEDGDGGGDGGGFBGDFGFGGDAGGGGGGGGGGGAEFGGFEFBGGEGDCCG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:67:1744:8605/1	0	MtDNA	3773	60	101M	*	0	0	TTTTTTGGTTAAGATCCGTGTATATTTTGTTATTTGGTTTATTGATTTGTTATGTTCAAATTTTTGTCATAAAAAGTTATAAAAATTTGTTAATTATTTCA	GGGGGGGGGGFGGEGFFFFFBDEBEGGGGFG8DGGDE@EEEEEE?GGGFFG?GBEGFEGEEGGGGFCCAGEEFFEDDBEFEGEFEEGGGEGEEGEEAFGFB	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1310:8213:55897	99	MtDNA	3943	60	100M	=	4215	372	ATTTATTACTTTGTATTAATAGTTTTATTAATTTCTAAGTTTTCTAAAACTAGGGGTTATAATTTTATTAATTGAGAAACAACATTAGTATTTTTAAATA	CCCFFFFFHHHHHFHHIJJJJJJIJJJJJJIJJJJIJJJHIIJJJJIJJJIJIJJJGIIIJHIIJJIIJJIIIJEGGIJJIIHHHHHHCEFFFFFDEEEC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:77:12526:7315/1	0	MtDNA	3981	60	101M	*	0	0	GTTTTCTAAAACTAGGGGTTATAATTTTATTAATTGAGAAACAACATTAGTATTTTTAAATATTCCATTTAGAGTTTCATTTTTTGTAAAAATTTTCTCAT	EEEEEEEDEBDDFFFFEFAEFFFFFFFFFFFFFFFFFDFFEFFFFFEFFDEFEFEFFFFFFDEFFFEFFFAEBE@EEFEFFFFFEBEEFEEDFEEEE?EBF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2311:10246:30315	99	MtDNA	3984	60	100M	=	4165	281	TTCTAAAACTAGGGGTTATAATTTTATTAATTGAGAAACAACATTAGTATTTTTAAATATTCCATTTAGAGTTTCATTTTTTGTAAAAATTTTCTCATTG	CCCFFFFFHHHHHJJIIJJJIJJJJJJJJIJJIJJJJJJJJJGJJJIBGIJJJJJJJJJJJJJIJJJJIJIHIJJEHIJJHFFFEDDEEEDDDE@CCDEC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:12:4535:13471/1	16	MtDNA	4089	60	101M	*	0	0	AATTTTTAAATATGATAGATTCTTTACTCTATTTTTGCTTTTTACAATATTTTTATCTGTATTGGCATTTAGATTTTGATTAATTAACTTGAGAATGAAAA	GGGGDADGFGFEGEBEFEFFFEEEEBEBC@EGGGGGEGFGGGGFGFG<GGGEEGGGGGGFGGGGFGGGGEGGGGGGGGGGGGGGGGGGGGGGGGGGGFEGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2311:10246:30315	147	MtDNA	4165	60	100M	=	3984	-281	TGATTAATTAACTTGAGAATGAAAAATAATGAAGAAACTTCAAATAATAATAAAATAAATTATTTTATTATTTTTCCGTTAATAGTTATTTCTATTATTT	EEEDFFFDFFHFGHHHHHHGHJJIJJIIHDIJJJJIHJJJJJJJJJJJIIJJJJJJIJJJJJJJJIJJJJJJJJHJIJJJJJIJIJJHHHGHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1310:8213:55897	147	MtDNA	4215	60	100M	=	3943	-372	TAAAATAAATTATTTTATTATTTTTCCGTTAATAGTTATTTCTATTATTTAATTACTTTTCTAGTAAAATATATTATATTATCTTGATAAGGTAAAGTTC	@AECC@BDEDFFFGHHECHJIJJJJJJIEJIHJIJHEGIJJJJIHIJJIIJIIHFIJIJIJJJJJJJJJJJJJJJJIIJJJIIJJJIHHGHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:6:13139:10288/1	16	MtDNA	4217	60	101M	*	0	0	AAATAAATTATTTTATTATTTTTCCGTTAATAGTTATTTCTATTATTTAATTACTTTTCTAGTAAAATATATTATATTATCTTGATAAGGTAAAGTTCCAG	EDCE=C==?B=DD?5@=CDDDBBDEEDEECE5EGGDEBDFEEBFGBEEEECE=AEEADGFFGF?GFGGGGFB?BE=BGDEFGGGFDFGGGDDFFFF?DEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:35:11810:7389/1	16	MtDNA	4285	60	101M	*	0	0	ATATTATATTATCTTGATAAGGTAAAGTTCCAGTTGGGAGAAGTAAGATGTAAAATAGATATTACTATGTTTGGTTACGGTCCAAAAAGATGCACATCTTT	DB===?B?EEB5B5EDC?:@EABB:?B@BCBAF5BFFFFFD=DDDFEFDFFFDDFEEE:BECE?=C=AE?EABE5EEFFFEEA8EE=BECEEBB=EFFEFF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:9:13349:5289/1	16	MtDNA	4305	60	101M	*	0	0	GGTAAAGTTCCAGTTGGGAGAAGTAAGATGTAAAATAGATATTACTATGTTTGGTTACGGTCCAAAAAGATGCACATCTTTGCGATCTAGTTTAGAAAAAA	=GEGGFGGEEGGGGFAFFGEGGGFGEGGAFGGGGFGDGGFEGFGGGFFGGGGGGAEFFFFBDGGGGGGGGDGFGGDGGGGGGGGGGGEGGGFAGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:31:10170:14522/1	16	MtDNA	4388	60	101M	*	0	0	GATCTAGTTTAGAAAAAATATTTGTTTTTGGTGCAAAAGAGTTTGATTGCATTTAGTTTACTCTTTTAGTTTATAATTAAAATATGGCCCTGAAGAGGCTA	EEE?DE5FDECCCC=FEE?AGEGGFFGGGFGGGFFGFGGEBBGGGGFGGBGGGDGGDDGGGGGGEGDGFGGDGGGFGDGGGGGFEGGGEGGGFGGGEGEGF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:59:7488:8665/1	0	MtDNA	4539	60	101M	*	0	0	TGGGATGTTGGTGACATTGCCATCTAGAAAAACTTTAACATTAAGATGAAATTTTGGTAGTATATTGGGTATAGTTTTAATCTTTCAGATTTTAACAGGTA	GGGFGFGFFGFEFDFFDFFEGEGGGGFGEFGGGD?GGDGGGGGGGGFFGAGEFDGDDBEEDFFFEGGGE@DEEEBCDFBEFGGFGFGFEDE?E?GFDEFAF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:49:13216:3175/1	0	MtDNA	4636	60	90M1D11M	*	0	0	GGTACATTTTTAGCATTTTATTATACGCCCGATAGGTTAATAGCATTTTCAACAGTGCAGTATATTATGTATGAGGTAAATTTTGGATGAACTTTTTAATT	DD@DDAEEEDGGFDGFGGGDGGGGGGEGFGBDFEFEDFFFEFFFDGGGFGADFFFBDDCECEECEFEFDCEFGEGEA?C=DDECED?EF@EAEEEEEEEFB	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:21:4522:8165/1	0	MtDNA	4827	60	101M	*	0	0	AGTATGAATGTCTGGTTTAACAATTTATTTATTAGTAATAATAGAAGCTTTTATAGGTTATGTTTTAGTTTGAGCTCAAATAAGATTTTGAGCAGCAGTAG	DEEDECBEEEBEEE=?EEEEFDAFFEFEE<EEDEEFEFDFDFFF?FFFBFEDDEEFFBF5D:BDDD>=ADEC:DAFFE=FFFBFBF?FFD:FDFFFDFADE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:84:1391:9200/1	0	MtDNA	4874	60	101M	*	0	0	CTTTTATAGGTTATGTTTTAGTTTGAGCTCAAATAAGATTTTGAGCAGCAGTAGTTATTACTAGACTTTTAAGAGTTATTCCAATTTGAGGGCCAACGATT	GFGGGFFGDDEBEEEFFFFEFFFFFGGGGGGGGEDGFGGFGGGGEGBGGGGFGGGGAG?EFFDGGFGGGG:EEAD=ECEEEGFGGFFFFEA?CEABB7BB@	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+EAGLE:2:107:7520:19689/1	0	MtDNA	4896	60	101M	*	0	0	TTGAGCTCAAATAAGATTTTGAGCAGCAGTAGTTATTACTAGACTTTTAAGAGTTATTCCAATTTGAGGGCCAACTATTGTTACTTGAATTTGAAGAGGTT	BGGGGADGGGGDGGFFAGGF:FFFFGFGFFFACDEFFFEBGF?GGGFGBEEC5DED==D??ECEEEFGGGFFEFBD=BAEDDBFBD:?BDEEDB?===D@?	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:31:10465:15263/1	0	MtDNA	4937	60	101M	*	0	0	GACTTTTAAGAGTTATTCCAATTTGAGGGCCAACTATTGTTACTTGAATTTGAAGAGGTTTTGGTGTTACAGGGGCAACATTAAAATTCTTTTTTGTATTA	GGGGGGGGDGGGEGGE=EEEDGDGGFGDGGGBGFGGGGGEGDGEEGEGGGGEDEGEEE=EEEDE=FAEFAEEFDEBGFEDEEEEBEBEEBEGEE7C:=5AB	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:29:14125:10562/1	16	MtDNA	5109	60	101M	*	0	0	AACATCTAGGTTATATTGCCACGGTGATTATGATAAAGTTTGTTTTAGACCTGAGTACTTAGGTAAAGATGCTTATAATATTGTTATTTGATTATTATTTA	ECGDEGEGEGB?FBGFEGGGCGEGDGFGFG?GGGGFEGG6GGGGDGGGEGGEEEE=EGGGEGGGGFBEGEGGBFGGGGDFFGFFGGGEDGGEGGGGGGGFG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:37:12921:1122/1	16	MtDNA	5174	60	101M	*	0	0	AAGATGCTTATAATATTGTTATTTGATTATTATTTATTGTGTTAAGGTTGATTTACCCGTTTAATTTAGGTGATGCAGAGATGTTTATTGAAGCTGACCCT	DAB=?B=BGGEGBEDGEGEGGGGDGEGGFAFBGEGGG5EGGEBGGGCE?E?GGECGDEFGGEGGGGFGGDGGGFGDGGGGFFGGGGDGFGGGFFGGDFFFF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2215:6882:11082	99	MtDNA	5381	60	100M	=	5391	110	GGATCGTAACATTTTATTTTTTTGCTTTAGTTAATAATTATACCTCTTGTTTAACCAAATTAAATAAATTTTTAGTATTTATATTTATTATCTCATCTAC	@@=DDDDDFHFDHIIHIJJJJJIJIIIJJJGIGIJGJJJIJJJJJIIJJH=FGHJICHHIJJGIJJIIHEEHHHF@EFFFFEEEEEEEEEFDDDCCCDD:	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2215:6882:11082	147	MtDNA	5391	60	100M	=	5381	-110	ATTTTATTTTTTTGCTTTAGTTAATAATTATACCTCTTGTTTAACCAAATTAAATAAATTTTTAGTATTTATATTTATTATCTCATCTACTATTTTAAGA	=CCCCBBBEEHEHCFIEAGGHIFGC@IGGHFCIIIGIGD@IHGIGIHEEIHFGIIIIIDIIIIIIIIGFC:IIIIHECIGEFEC<GIHFFB?DDDDD@@@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:70:6358:2346/1	0	MtDNA	5567	60	101M	*	0	0	CTTATTTAATATTGTTTATTTTTATAAGAAGTAAATTGTTATTTAAATAATAAAACATGTCTAGTATAAGTTAATACATTAGATTTAGGTTCTAAAGATTT	FFGBFGFGGDGGDFGGGDEACCACAGGGGEDEGFGGDFFGDEGGDGGFG?GGGGGGEGGAEEDEBFDFDFEEGDBFFFFFDGEEDEBEE@EGEEEDEDDEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:5:6773:20401/1	0	MtDNA	5728	60	101M	*	0	0	ATTTTTTGCCTCAGCCGGAATGTTAAGATCTTTAGTAATATTTTTTAAATTTGGACTATACGAATTATTTATTTTTACACTATTTTCTGTGTTATTTATTT	GGGFFFEFFGGGDGGGGGGFGGGFGGGGGGGGGEFFGGFGGGGGGGGDFGFFFFDEFDFBEEEDBEEEEEAGGGGDCEGGEDGGDGGDGFGGGDGGGDGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:39:10545:13693/1	16	MtDNA	5756	60	101M	*	0	0	TCTTTAGTAATATTTTTTAAATTTGGACTATACGAATTATTTATTTTTACACTATTTTCTGTGTTATTTATTTCTTTTGCTTGAGGTAAGGATATTGCTAT	FADAAEEEF;CDFAEEEEFEAEEEB@>CCCA:EE?EF=A@ECFFFFD?EEEEDEE:EFFEFEEEBAC;FECCDBDEEEBA??F?DFAFFFEEDDE=DDAD:	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:96:18019:19876/1	16	MtDNA	5769	60	101M	*	0	0	TTTTTAAATTTGGACTATACGAATTATTTATTTTTACACTATTTTCTGTGTTATTTATTTCTTTTGCTTGAGGTAAGGATATTGCTATAGAAGGTTTAAGA	GEBBFGGFEEGEE=EBGFEGGEEEEADDGGGEEEEBEAFFEFFDEDGG?GGGFGFG@DGGFGGBGFGGEGFFGGGGGGDFFGBGBGGGGGFGFGGFDDFEF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:28:5015:7424/1	0	MtDNA	5798	60	101M	*	0	0	ATTTTTACACTATTTTCTGTGTTATTTATTTCTTTTGCTTGAGGTAAGGATATTGCTATAGAAGGTTTAAGAGGTTACCATAATTTTTTCGTTATAGACGG	GGGGGGFGDGGGGGGGGGGGGGGGGGGGGGGGGEGGGGGGGGGGBFFFFFGGGGGGGGGGGGGGGBGGFGFEFFCFAEFFDDGFGGFGFEGEGFFDFDDFF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:30:5276:1934/1	0	MtDNA	5801	60	101M	*	0	0	TTTACACTATTTTCTGTGTTATTTATTTCTTTTGCTTGAGGTAAGGATATTGCTATAGAAGGTTTAAGAGGTTACCATAATTTTTTCGTTATAGACGGGTT	GGGDGGGGEGGGGGGGFGGGEGGGGGGGGGFGGGGGGGGGGDGDGFCFCFGGGGGGDGDGGGDGG=EDBEFAFDEDEFBEGGGGEGEGEFCECEC?EEECC	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1104:14433:23059	99	MtDNA	5853	60	100M	=	5996	243	CTATAGAAGGTTTAAGAGGTTACCATAATTTTTTCGTTATAGACGGGTTTAAATTCGGAGTAATTTTATTTGTGTTTAGGGAGTTTATATTCTTCTTTTG	BCCFFFFFHHDFHIJJIHJFHJJJJJJJJJJJJJJJIJIJJJIJJII@FIHIIIIIJJHH?AEHHHFDFFFFFEA6@>CDD;?<CCCDDDFEEEDDCDD:	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:83:3624:6531/1	16	MtDNA	5868	60	101M	*	0	0	GAGGTTACCATAATTTTTTCGTTATAGACGGGTTTAAATTCGGAGTAATTTTATTTGTGTTTAGGGTGTTTATATTCTTCTTTTGTATTTTTTGAACATTT	BEEDFDEGDEEFEEDBDFEGFGGFGGFBGFGFAGGFGGGFGGGEGGG>EGGGGGGFGGGGFEEEEF;EFEEGGGGFGGGGGGGGGGFGGGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+EAGLE:1:76:4139:2647/1	16	MtDNA	5966	60	101M	*	0	0	TTTTTTGATGCTGATCTAGTACCAGTACACGAGTTGGGAGAGACTTGATCACCTTTTGGAATGCACTTAGTTAATCCATTTGGTGTACCGTTATTAAATAC	EBBEEEEEEEE@B@BEB@@B?BC?BB@B?DEEEDEFDBEEEB=CCCE@EE@FFFFDECEEDEDEBEFFEFDFFFEFEAFDFFEFCFBDFFFFFFAFGGGBG	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:1104:14433:23059	147	MtDNA	5996	60	100M	=	5853	-243	GAGTTGGGAGAGACTTGATCACCTTTTGGAATGCACTTAGTTAATCCATTTGGTGTACCGTTATTAAATACAATTATTTTATTAAGGAGTGGTGTTACAG	CDDDCCAECEC@EEFFFFFDAHHGJJJJJIJIJJIJJJJJJJJJJJJIGHHGJJJIGJIJJJJJIJJIIHJJJJJJJJJJIJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:99:8553:16567/1	0	MtDNA	6020	60	101M	*	0	0	TTTGGAATGCACTTAGTTAATCCATTTGGTGTACCGTTATTAAATACAATTATTTTATTAAGGAGTGGTGTTACAGTAACTTGAGCACATCACAGATTACT	:FDGBFGFGDDGFDGFFGGFBGGDGFFFF=EAEEEDEEAEFDFDFGFG?GG?FEGGGFGGGGGFFDFFEFEAFFFGEGGGGAGEFGEFGGFGGECBEDDFG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2201:8667:82934	99	MtDNA	6028	60	100M	=	6185	257	GCACTTAGTTAATCCATTTGGTGTACCGTTATTAAATACAATTATTTTATTAAGGAGTGGTGTTACAGTAACTTGAGCACATCACAGATTACTTAGAAAT	CCCFFFFFHHHHHJJJJJJJJFHGHIIJIJJJJGJJIIJJJIIJJJJJIJJIIJJJJBFIBFGHIEIGEGHJJJJJHHHHGHFFFFFDECECEEEDDDDC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:83:16211:16889/1	0	MtDNA	6029	60	101M	*	0	0	CACTTAGTTAATCCATTTGGTGTACCGTTATTAAATACAATTATTTTATTAAGGAGTGGTGTTACAGTAACTTGAGCACATCACAGATTACTTAGAAATAA	EAEEBGFGGFGGGGEFFFAFEFFGEGFEDGDEECCGFDFBGGEGFGG@FGCBFDDFDFDBBDDBBBEBEFBAEEBFBDFEEGEEEB=B@BBEB?BAABDBE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:8:8735:6148/1	16	MtDNA	6076	60	101M	*	0	0	ATTAAGGAGTGGTGTTACAGTAACTTGAGCACATCACAGATTACTTAGAAATAAAAGATGTACTAATAGTATAATTTTAACATGTTTATTGGCAGCTTATT	DB:DDDB?D:BDCDDDD?DDDD=DADDADDBDDDDBDDDDDDADDDBC>CDDADADDD:DBCCBDDDDADDDDDBD5DDDDE?DEE:EFFEEEBDD;EEE:	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2201:8667:82934	147	MtDNA	6185	60	100M	=	6028	-257	ATTCAATTAATAGAGTATATAGAAGCTAGATTTTCTATTGCAGACGGAGTATTTGGAAGGATTTTTTATTTATCTACGGGATTTCACGGAATTCATGTCT	CCACEEFEECDCDEEEEEEEDEFFFFFFEGHHHHIJJIGJJHHDJIJJIHHJJJJJJJIGGJJJJJJJIJJJIIHGJIJJJJIIIGJHGHGHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:105:1340:12432/1	0	MtDNA	6235	60	101M	*	0	0	ATTTGGAAGGATTTTTTATTTATCTACGGGATTTCACGGAATTCATGTCTTGTGTGGTGGTTTGTTCTTAGCATTTAATTTTTTACGTCTTCTAAAAAATC	FFFD@DGGEEEFGGGGGGDFGGGGGGGEGGFEFFEEDEE:EADDEFF5FBEE=EEDDADDB?DE=><:6>EEDEE:D5EDEEBB5:ECAC:?B:CEBEEEC	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:54:12468:5717/1	16	MtDNA	6304	60	101M	*	0	0	AGCATTTAATTTTTTACGTCTTCTAAAAAATCATTTTAATTATAATCATCATCTGGGGTTGGAATTTGCTATTTTATATTGACATTTTGTTGATGTAGTGT	BBB:DDC?CBCB@AB:BDDDD@C?>CACB::?DCD=CC:?A=D:DB?DDAD:?CCCD=D:DDDDA?ADDDA;C:AAC?=A??:@<DC5CC=A5CCA6;@=;	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:41:9266:7772/1	0	MtDNA	6313	60	101M	*	0	0	TTTTTTACGTCTTCTAAAAAATCATTTTAATTATAATCATCATCTGGGGTTGGAATTTGCTATTTTATATTGACATTTTGTTGATGTAGTGTGATTATTTT	GGGGGGGGGGEDFGGGGGFGGDGGEGFFEGFGFGEGGGGGGFGGGGGGGEFGFDEDGGGGGEFGGFBGGGGGAEBBFFFEEEEBEEBBEAEAEABGEEDGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:65:9093:5871/1	16	MtDNA	6392	60	101M	*	0	0	GTTGATGTAGTGTGATTATTTTTATTTGTGTTTGTTTATTGATGATCTTACTAATATAGCTATGATAGTTTAATTTAGAATATATAACTTGTAATTATAAG	FBEEBE??:B@D:FAFFEAAFFFFFFFFFAEFFEFCFFFFF?FEFFDEEEDDFFEFCCCC:DDDDADEEEEDDBEEEEED=FFFFEEFDGGGGDGGEGGBG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:89:15660:12072/1	16	MtDNA	6413	60	101M	*	0	0	TTATTTGTGTTTGTTTATTGATGATCTTACTAATATAGCTATGATAGTTTAATTTAGAATATATAACTTGTAATTATAAGGTTTCTAGTAGCTTTGTTAGA	BEEAEEEGEGEEFGEGFGBGDFGEBFGEEGFGGFGGGGGFFFFEDCEGGGGEDEFFGGGGDEEED?AGFGGEGDGFGEFGGEGGGFGGGGGDGBGGGAFGF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1210:8873:86961	99	MtDNA	6488	60	100M	=	6541	153	ATAAGGTTTCTAGTAGCTTTGTTAGAATTTCTTTTTATCTCCTTATTATGACTTTTTAAACCTATTTATTTTTTATTATTTACAGTGATGTTTAGGTTTT	@B@FFFBDBHHFHEGGIGIECEHIJGHIIGGIIIJIGIJIJGDHHHIJJEDEEGIIIIIIJJJJJJJJJIGGIIJJIHDECHEEE?DFF@?CFECE>CCB	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:35:14137:12613/1	0	MtDNA	6539	60	101M	*	0	0	CTTTTTAAACCTATTTATTTTTTATTATTTACAGTGATGTTTAGGTTTTTAATTTTTAATAATTTTTCATGAGGCGGACTATTTTTAGTATTAGATTCATA	GGGGGGGGGGGGDGGGGGFGGGGGGGGGGFFGFGDGCGGECFFFF?FEFDEAGGGAGGGGGGGGGGDGFGGEFFGGGEEGEGDGGE@GEEGEEFEEAEED@	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1210:8873:86961	147	MtDNA	6541	60	100M	=	6488	-153	TTTTAAACCTATTTATTTTTTATTATTTACAGTGATGTTTAGGTTTTTAATTTTTAATAATTTTTCATGAGGCGGACTATTTTTAGTATTAGATTCATAT	CACCCCCCCDA@CCA<EEEHFHEA=>CAIGIGH@;F9EEFC1EIHHCEHGBCIHFAGD9HD?9DCFFCGCCA@A,ACGEIIGGGEDE?F?DFABD7D@@@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:80:12688:9364/1	0	MtDNA	6564	60	101M	*	0	0	TATTTACAGTGATGTTTAGGTTTTTAATTTTTAATAATTTTTCATGAGGCGGACTATTTTTAGTATTAGATTCATATAGATTTATTTTATTGATTGTTATA	GGGEGAFCDDFAFDFEECDE@EEDEEFFFBDDDD:A?AC?GDGEFFGFEACEB:C=EEEE>:C@BEEEE=DEFA?DFEE?DE??EFC>5B;?AAEDAD9D=	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:83:3138:12153/1	16	MtDNA	6612	60	101M	*	0	0	GCGGACTATTTTTAGTATTAGATTCATATAGATTTATTTTATTGATTGTTATAAGATTATTTATTTTAGGTATTATTGTTATTAGAGAAAAAAATAATAAT	GGGFEEGFEEEBEDGGFGFFGGGGGFGFGGEGFGDGGGGGGAGGFGGGGGGGGGGGGGGFGGGFGGGGGGGDGGGGFGGBFGGGGFGGGGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:59:15380:6072/1	16	MtDNA	6635	60	101M	*	0	0	TCATATAGATTTATTTTATTGATTGTTATAAGATTATTTATTTTAGGTATTATTGTTATTAGAGAAAAAAATAATAATTTATTGATTTTATCTGAAATTTT	EDGEEBEE@EEGEEEEEEEAEEE?EEEEAEBFEEFEEEGGGEEGDEEFEDDGFEECGGEDGEGEDGEGDGEGFGEGGGFEECDCD;G=GFFAGGGGGGGGF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2109:14592:38248	73	MtDNA	6659	60	87M1I5M1I1M1I4M	=	6659	0	GTTATAAGATTATTTATTTTAGGTATTATTGTTATTAGAGAAAAAAATAATAATTTATTGATTTTATCTGAAATTTTAGTATTTATTCTGTCTCTTATAC	CCCFFFFFHHHHHJJIJJJJIIICHJJIJJIJJIJJJJJJJJJJJJJJIIJJIJJJJJJIEIJJIIJJJJIGGHJGHHHFHHHFFFFFFFEEEEEEDEDD	PG:Z:SNAP	RG:Z:000	NM:i:6
+BS-DSFCONTROL03:121:D0M8KACXX:5:2109:14592:38248	133	MtDNA	6659	0	*	=	6659	0	AATAAATACTAAAATTTCAGATAAAATCAATAAATTATTATTTTTTTCTCTAATAACAATAATACCTAAAATAAATAATCTTATAACCTGTCTCTTATAC	CCCFFFFFHHHHHJJJJJJJIJJJJJJIJJJJJJJJJJJJJJJJJJJJJJHIJJJJJJJJJJJJJJJJIJJJJHHHHHHHFFFFFFFDDECEDDDDDDDC	PG:Z:SNAP	RG:Z:000
+EAGLE:1:39:10037:14104/1	16	MtDNA	6823	60	101M	*	0	0	TATAATTCTAGGTTATGGTTCTCAAATTGAAAAAATTAACTCCTCTTATTATTTAATATTTTATGCAGCTTTTTGTTCATTCCCATTTTTATTTGTATATT	E?F?FDFGF?FGAGAGFFFEDGGDGGGFGFGFFF?FG?EGGDDBDBAF>EFEGGGGCGDGFGGFGGFGGGFGGFGGFGGGEDGGFGGDDGFFGEGFFGFDF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:51:9949:15014/1	16	MtDNA	6862	60	1M1D6M1I87M1I5M	*	0	0	CGATCTTTATTATTTAATATTTTATGCAGCTTTTTGTTCATTCCCATTTTTATTTGTATATTTTAAGAGTAATTTTTTATTGGTTTTTTCTTACTTATTAC	EEEEEEEBEEEEEE=EEEEEBEEEEEEEE=C?EEDDEEDEE?EEECCCCEDDEEEEBEEEEEEEEAECEBACAC<EEEEE=ECDEED?DDBE5AEEEEEDE	PG:Z:SNAP	RG:Z:266-1	NM:i:8
+BS-DSFCONTROL03:121:D0M8KACXX:5:2210:19195:39418	83	MtDNA	6948	60	100M	=	6949	101	GTACTTACTATAATTTTGTTATTTCTTGAGAAATATTTTTTATTCTTAGTTTAAGATTTATAATAAAATTTCCAATTTATTTTTTACATCTTTGATTACC	CCBDFDDDEEEDEDEFFFFFFHHFHHIJJJJIJJJJIJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJIHIJHHHHHFFFDD@BB	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:2210:19195:39418	163	MtDNA	6949	60	100M	=	6948	-101	TACTTACTATAATTTTGTTATTTCTTGAGAAATATTTTTTATTCTTAGTTTAAGATTTATAATAAAATTTCCAATTTATTTTTTACATCTTTGATTACCC	CCCFFFFFHHHHHJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHIGIJJJJJJJJJJJJJJJJJJJIIJJJJIJJJJJFFFFFFEEEECEEEC>;	PG:Z:SNAP	RG:Z:000	NM:i:1
+EAGLE:1:86:3647:16273/1	16	MtDNA	6985	60	101M	*	0	0	TTTTATTCTTAGTTTAAGATTTATAATAAAATTTCCAATTTATTTTTTACATCTTTGATTACCTAAAGCTCATGTAGAGGCTCCTACAACAGCTAGAATAC	<DEBBBEEEEBEBBEEEEEEEEEEEG?FGEEDD?EBEEEEEEEFEDEDAFEBDDEEEDFFADCBCCFABFEGFGDGEFEDGEFFFEFDFFFEFFFFDEEBE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1204:10706:29128	99	MtDNA	7118	60	100M	=	7195	177	GCGGGATTTTTACGTATTTTAGGTAGTTTAAGATTTGTAAAAAACAATGTATGAATTTTTATTGCATTTTTAGGGATAATTTTTGGATTTTTTTGTTGTT	;;<@################################################################################################	PG:Z:SNAP	RG:Z:000	NM:i:6
+EAGLE:2:99:13522:17668/1	0	MtDNA	7120	60	101M	*	0	0	GGGATTTTTACGTATTTTAGGTAGTTTAAGATTTGTACACAACAATGTATGAATTTTAATTGCATTTTTAGGGATAATTTTAGGATCTTTTTGTTGTGTGT	GFDGFGGEAFEFBFF?FFFFDBDF?ECECEEGDFGEGGGGFEEDDFGEGEBDEFDBEEFBEGFDEFEEE9EEC@?EEEEEDEGEEEEFEEGGEEEABBAFF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1204:10706:29128	147	MtDNA	7195	60	100M	=	7118	-177	AATTTTAGGATCTTTTTGTTGTGTGTTTCAAAGAGATTCAAAGGCTTTAGCAGCTTATTCTTCAGTCACTCATATAAGGTTTTTATTATTATCACTGGTA	CEDDDCC@;DBCCDEBFDFHFFJGEGIGGIJJJIEIIGHEIHGJGJJIHFIHACGGEGIJIGIIHHEFIGEJIJGIHEJIJJIGIGGGGHHHED>FDC@@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:102:7363:18384/1	0	MtDNA	7214	60	101M	*	0	0	TGTGTGTTTCAAAGAGATTCAAAGGCTTTAGCAGCTTATTCTTCAGTCACTCATATAAGGTTTTTATTATTATCACTGGTATTTATCACAATGAGTAGTAA	D:BDDCEDEEBFFFFGGEGGGGGGGGGGG:EEEEEGGGGFGFGGCGEGGFGDGGGGGEEGBEEEEFGGEGEAFE:FFDFCGGGGGFFADDFFFFFAFFEEF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:38:3103:6670/1	16	MtDNA	7304	60	101M	*	0	0	ATGAGTAGTAAAATTAGAAGAGTTATGCTTATGCTAGCACACGGTTATACATCTACACTTATGTTTTACCTTATTGGTGAATTTTATCATACAACTGAAAA	FGGEGGGGGGGGGAGGGGFGGGEEGGGGGGEEGGGGGGFGGGGGGGGGFGGFFFDFEGGGGFGGGGGEGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+EAGLE:1:55:13359:5768/1	0	MtDNA	7379	60	101M	*	0	0	GGTGAATTTTATCATACATCTGGGAGGCGTATAATTTATTTTATAAGTAGATTTTTTAGATCTAGAATAATTATAGGTATTTTGTTTTCAGTTGTATTTTT	FBFEFFFADEGBGDGDGGGGGFFGGGGFGDGEBBGGGFFGGGGAGEEBFFFGEGFGGEBEGGGBGEEGDFDGFGEGGEAGFGGGDGFEEBCEEECEFGGG?	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:40:8204:14676/1	16	MtDNA	7463	60	101M	*	0	0	TTTTCAGTTGTATTTTTATCAAATAGGGGTGTACCACCTTCTTTGTCATTTTTATCAGAATTTTTAGTTATTTCTAATAGTATATTAATTAGAAAAAGTAT	DDBEBGED?FGGECGGECDE;FGEGGFGGDGFDFFCFF>FFGGGGGDGGGEGGGGGGGGGFDGGDGGD?GGDGGGGGGGGGFGGGGGGGEGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2202:17139:67717	99	MtDNA	7469	60	100M	=	7536	167	GTTGTATTTTTATCAAATAGGGGTGTACCACCTTCTTTGTCATTTTTATCAGAATTTTTAGTTATTTCTAATAGTATATTAATTAGAAAAAGTATATTTG	CBCFFFFFHHHHHIJJJJJJJJJEHGHIIIIJIIJJJJJIJJJJJJJJJIIIIHIJJIJIIGIJJJJJJEGHGGCCEHHHHHHHFFDFDDCD;CCBDDE>	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2202:17139:67717	147	MtDNA	7536	60	100M	=	7469	-167	CTAATAGTATATTAATTAGAAAAAGTATATTTGTAATAATTTTTATTTATTTTGTAGTATCTTTTTACTATTCTTTATTTTTGATTACAAGATCTTTAAT	DEEEEFFFFFFFFHHHHHHJJJJIJIJJJJJJJJJJJJJIJJJJJJJJJJJIJJJJJJIIJJJJJIGJJJJJJJJJJJJJJJJJJJIHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:57:7347:8572/1	0	MtDNA	7620	60	101M	*	0	0	TTACAAGATCTTTAATAGGAAAAGGTTACCATAATTTTAATACTTGAAATGTAGGGTTTTCAGCACCATTAGTCTTAATAATATATAATGTATTTTGATTA	FEFFDEBE??EEDEAFEDFDFFFDEBBDEEC?CCD=EEEBFFEEFF?FF=?:?ACE?BCEFFFFDFEFDEFEEEDEBDDEEEDEEEDDED@B=ABEDDBEB	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2114:12015:97588	99	MtDNA	7885	60	100M	=	7994	209	GATTAGAGAGATCTAATCATAAAGATATCGGAACTCTTTATTTTATTTTTGGACTTTGATCTGGTATGGTTGGTACTAGATTTTCTTTATTAATTCGTTT	B@@FFDFE>FFHHIJJIGIIJJIGHGJJJFIIIJJJJJJHJIJJGIJJJIGHIGIIJJIIIJIGCGHGDEHIE?CEFFFEFFEDEEEEDEDCDEEDDDDD	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1314:9064:66647	99	MtDNA	7912	60	100M	=	8034	222	TCGGAACTCTTTATTTTATTTTTGGACTTTGATCTGGTATGGTTGGTACTAGATTTTCTTTATTAATTCGTTTAGAATTAGCTAAACCAGGTTTTTTTCT	BB<1:=@BFHFH>CFHB<F<CHD@:FB?HEA<?CADHI@DFHHBEB0?FGCFHEAFEEEHI@FFDHJJ@;@=@@>>EC;=;@EEB;@>ACD555=?=BDC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2114:12015:97588	147	MtDNA	7994	60	100M	=	7885	-209	TAAACCAGGTTTTTTTCTTAGGAATGGACAGTTGTATAATTCAGTTATTACAGCTCATGCTATTTTAATAATTTTTTTTATGGTAATACCTACTATAATC	CBC@DBCBDDDDDEDEEEEFFFFC?BDAFHHEIJJJJIHGGGIIGIIIIHJIIIGFJIIGJJJJJJIIGIIIJJJIIIIJIJJJJIGFGHHFFFFFFC@C	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1314:9064:66647	147	MtDNA	8034	60	100M	=	7912	-222	TCAGTTATTACAGCTCATGCTATTTTAATAATTTTTTTTATGGTAATACCTACTATAATCGGTGGTTTTGGTAACTGATTATTACCACTTATGTTAGGAG	:::@@B;5AA:@:@5:A>>A>;B@@@>DA>@?6AC;7C@=8B<FCF>D<FF?IIG@GG?@DGEGD@EA<4@F?<AC3,<F:<:A@C:FBF8?AD?DB@@=	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:86:14256:19867/1	16	MtDNA	8225	60	101M	*	0	0	TATAGGTTGTGGGACTAGGTGAACAGTCTACCCACCTTTAAGAACAATGGGGCACCCTGGAAGTAGAGTAGATTTAGCTATTTTTAGTTTACATGCAGCAG	=BBB@BBBBBDBDBBBAD@ABDADBDABA6BBDBDBDDDDEEGEEGDDFEFEE@EEEGGGFDGGFGGGGGGGGGFGGGFGGEGGGGGEGGFGGGEGGEGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2214:18868:93712	99	MtDNA	8250	60	100M	=	8386	236	GTCTACCCACCTTTAAGAACAATGGGGCACCCTGGAAGTAGAGTAGATTTAGCTATTTTTAGTTTACATGCAGCAGGGTTAAGATCTATCTTAGGTGGTA	11:BA::2220CFH@>94:<<:H@BBEHHGHHGGI?GEH>?FADFGIGIICGGEGIBAHGIICFHEEC>DGEHGGHFF@BDEE@CACCCCDDDDD::<?3	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2214:18868:93712	147	MtDNA	8386	60	100M	=	8250	-236	GTTCTATTTCATTAGAACATATAACTTTATTTGTTTGAACTGTGTTTGTAACAGTGTTTTTACTGGTTTTATCTCTACCGGTTTTAGCAGGGGCTATTAC	AB>:>A>@A>@;EEEEDDEEEDAB@DDDDDB;?=?:IEECAEC;@CBDF==FCFB?FBBD<G=BDDDBAGEG@FGC<GFAHI<AEA;C:<==;D?DB@=@	PG:Z:SNAP	RG:Z:000	NM:i:1
+EAGLE:2:39:11175:12286/1	0	MtDNA	8405	60	101M	*	0	0	TATAACTTTATTTGTTTGAACTGTGTTTGTAACAGTGTTTTTACTGGTTTTATCTCTACCGGTTTTAGCAGGGGCTATTACTATGTTGTTAACTGATCGTA	BDDD?BDDDDEEEEFFFFEFFFFBFEEDEBEEEEEDFEFFFCCF:EE@EEFDEFDFFEFFFFDEFBEDEBDD:FBEEEEBEEEEAADDBABDADFB?C=<B	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+EAGLE:2:113:8946:21007/1	0	MtDNA	8550	60	101M	*	0	0	CTTATTTATCAACATTTGTTTTGATTTTTTGGTCATCCTGAAGTATATATTTTGATTTTACCAGCTTTTGGTATTGTCAGACAATCTACACTTTATTTAAC	GGDGGGGGGGDAGBEGGGFGGFGGFGGGEGDGEG=FFFEFGEFGEGFEEGGGGGFDGGGGG?FGEGGGGGECEFFFEEEGBEE?DFEADC?DEE=BE?<B5	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:81:3391:15213/1	16	MtDNA	8553	60	101M	*	0	0	ATTTATCAACATTTGTTTTGATTTTTTGGTCATCCTGAAGTATATATTTTGATTTTACCAGCTTTTGGTATTGTCAGACAATCTACACTTTATTTAACAGG	?DEDDDGEBE@EEFEFFGGGEGGGFGGGGEFEEGGGGGFGEGGGGGGGGGGFGGGGEGGFEEFFGGGGDGGGGFGGFEGGGGGGEFFGGDEGGGGGGFEGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1207:2101:11733	99	MtDNA	8591	60	100M	=	8633	142	AGTATATATTTTGATTTTACCAGCTTTTGGTATTGTCAGACAATCTACACTTTATTTAACAGGAAAAAAAGAAGTTTTTGGTGCTTTGGGTATAGTTTAT	BC@DFFFFHHHHGGJGJJJJJJIJJJJJJGDHIJGIEGIIIHGGIICGCDDG>GIJIIGG@DHHG9FGIBHFBEDDFEDD?BDDDDDDCB5>>CEDCDCA	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1207:2101:11733	147	MtDNA	8633	60	100M	=	8591	-142	ATCTACACTTTATTTAACAGGAAAAAAAGAAGTTTTTGGTGCTTTGGGTATAGTTTATGCAATTTTAAGAATTGGTTTAATTGGTTGTGTAGTATGAGCT	DDA>@C@DCAEECC>CDCDDDDDHHHHHHEGHEEGGJJIJHGGJIGGGIIHCIGIIJIIGHAIJJIJJJJIGIIIJJJJJIIIJJJIHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:25:12122:5259/1	0	MtDNA	8665	60	101M	*	0	0	TTTTTGGTGCTTTGGGTATAGTTTATGCAATTTTAAGAATTGGTTTAATTGGTTGTGTAGTATGAGCTCACCATATGTATACAGTAGGTATAGATTTGGAT	FGFAGFDBFD:EEEEEB5AEEEEEAFFFAFDF=FEFEFFFFDAEEFFFFFD:FFACEB:E?:BD?B?EBAFADBBDDBCEBBBAEBEE@EBBE@BFFE?AF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:82:4393:14891/1	0	MtDNA	8674	60	101M	*	0	0	CTTTGGGTATAGTTTATGCAATTTTAAGAATTGGTTTAATTGGTTGTGTAGTATGAGCTCACCATATGTATACAGTAGGTATAGATTTGGATTCACGTGCT	DGFDDEEAEEEECEEFFEDFGGGGGGEGDFEEEDEDEDBEGEGDGEEEEEFDFFFGGGCBFGGGBFFFDDFDFEFCEEE5@B?D:?CBDB5@B?@CFCF?B	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:3:4300:2958/1	0	MtDNA	8776	60	101M	*	0	0	ATTTTTCGGCTGCTACTATAGTTATTGCAGTGCCAACAGGTGTTAAAGTGTTTAGATGATTGGCTACATTATTTGGTATAAAAATGGTATTTAATCCACTT	GGGGGGGGGGGGGGGGGGGFGFGGGGGGGDDFFGGEEEEECEEEEECE@EFFFBFAEGBFGGFFFFDEDGCGGGBD=@@D:AA;9EE??=AA@C55>;<?E	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:75:3836:14170/1	0	MtDNA	8880	60	101M	*	0	0	TTGTGAGTATTGGGTTTTATTTTTTTGTTTACTTTAGGTGGGTTGACAGGTGTTGTATTATCTAATTCAAGATTGGATATTATTTTACATGATACTTATTA	EEECEEEEEEDDDDBGGGDGGGGGEFGDFGFGGGFGFG@ACC<CCBEEEE?EEAEDFFFFGGGFGGGGFEGBEGGDEGGEGEGGGGGGGGGEGGFFGGFGD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:57:17429:11489/1	0	MtDNA	8902	60	101M	*	0	0	TTTTGTTTACTTTAGGTGGGTTGACAGGTGTTGTATTATCTAATTCAAGATTGGATATTATTTTACATGATACTTATTATGTAGTTAGACATTTTCATTAT	GGGGGGGGEGGGFAGFDFFFEEFFFFFFCFFFFDFEBBFEGFGGGEEEGECGGGCGEGEAEGGEGGBGGEDEEFEDGGDGACCECFDBBCFEFEFD@AFA@	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2312:8770:13442	99	MtDNA	9086	60	100M	=	9229	243	ACTTATGATATCTGCAGTATTTATTTTATTATTTATTGGGGTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATAT	CCCFFFFFHHHHHJJJJFHHJJJJJJJIJJIJJJJJJJJJJCFHIJJJJIGJJJJJJJJJIJJIJJJJJJIIIGHEHHHFFFFDDDDDDDDDDD?ABFDC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1314:14393:27976	99	MtDNA	9087	60	100M	=	9151	164	CTTATGATATCTGCAGTATTTATTTTATTATTTATTGGGGTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATATT	@C@FFFFFFHHHHJJJGIIIJIJIIJGHIGIGIIIIJGIJ?AGHJGIIJHIIJIJJJJJIJJIJJJJJJIGIGGEAEHEDFDDD>?BDDCDDBDDBDEDC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:50:14046:18753/1	16	MtDNA	9087	60	101M	*	0	0	CTTATGATATCTGCAGTATTTATTTTATTATTTATTGGGGTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATATTT	BEEEEEEEEEGEEEEEEEE?D@EGGGEEEEGEEEEEGEGEEGDEGGEEEGEGGFGEGGGGGGEGGGGGDGGGGGEGGEGGGGGGGGGGGGGGGGGGGAGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2202:10705:30822	99	MtDNA	9102	60	100M	=	9126	124	GTATTTATTTTATTATTTATTGGGGTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATATTTAGATTACCCTGATG	CBCFFFFFHHHHHJIJJJIJJJJJJCFHIJJJJJGHIJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJIHBEFFFEBBDDDDEEFFEFDDDEDDDDDDDDA	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:72:10661:11179/1	16	MtDNA	9109	60	101M	*	0	0	TTTTATTATTTATTGGGGTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATATTTAGATTACCCTGATGTTTATTCG	;EEBEEEEEEEAEEEEEEEEEEEEEEBEDDD@E?EEGEEBEEDEEGDEEDEDCFFCECFFEGGEEGDEEGEFGGGGEFGFGGFGCGGDGGGGGGFGGFFGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2202:10705:30822	147	MtDNA	9126	60	100M	=	9102	-124	GTAAATTTAACATTTTTCCCGCTACATTTTGCAGGACTACACGGGTTCCCACGTAAATATTTAGATTACCCTGATGTTTATTCGGTATGAAATATTATTG	EDDEEDEEEDDDDDDBBDBAADEFFFFFBHHHHHHGJIHIIJJJJJJIIHJJJJJJJJJJJJJJIHG?JJJJJJJJJJJJIJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1314:14393:27976	147	MtDNA	9151	60	100M	=	9087	-164	ATTTTGCAGGACTACACGGGTTCCCACGTAAATATTTAGATTACCCTGATGTTTATTCGGTATGAAATATTATTGCCTCTTATGGTTCTATTATTAGAAC	CCC@DDCDDEDCCDDBDDDFDBFGFEIJIIGIGJJIJJIHGC=HIIIGFCJJJIIIIGJFIJJIJJJJFIHGJIGDHGIIIGHGIGIHHHHFFFFDD@@@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:52:2501:13323/1	0	MtDNA	9191	60	101M	*	0	0	TTACCCTGATGTTTATTCGGTATGAAATATTATTGCCTCTTATGGTTCTATTATTAGAACTGCAGGACTATTCTTATTTATTTATGTATTATTAGAGTCTT	GGFFGGFGEGGGFGGAFFFFGGGGGGGGEGGGGDGGGGGGGGGGFEGGGGGGGGGGGGGGGGGGGGEGGEGGGGGGGGGEGGGEGGEGGGGGGEFEFCFGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:46:14009:4930/1	0	MtDNA	9196	60	101M	*	0	0	CTGATGTTTATTCGGTATGAAATATTATTGCCTCTTATGGTTCTATTATTAGAACTGCAGGACTATTCTTATTTATTTATGTATTATTAGAGTCTTTCTTT	EEDBEDEFEFFFFFFEDEEBFFFDFECBE?BEEEEDCCEAEGDG=FEEDFAA:BEB=BBCA=AAAEAFBFFEGGBDF?=FABCCBEAGD=????@EEABE;	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2312:8770:13442	147	MtDNA	9229	60	100M	=	9086	-243	CTTATGGTTCTATTATTAGAACTGCAGGACTATTCTTATTTATTTATGTATTATTAGAGTCTTTCTTTAGTTATCGTTTAGTAATTAGAGATTATTATTC	CDEEDDEFFFFFFFHHFHHHFJJJJJJIHJJJJJJJJJJJJJJJJJJIIIJJJJJJJJJJJJIJJJJJJJJJJJJIJJJJJJJJJJJHHHHGFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2202:8985:17141	99	MtDNA	9258	60	100M	=	9340	182	CTATTCTTATTTATTTATGTATTATTAGAGTCTTTCTTTAGTTATCGTTTAGTAATTAGAGATTATTATTCTAATAGAAGACCTGAGTATTGTATGAGTA	CC@FFFFFHGHHHJJJIJJJGJJIJJIIJJHJJJJJIJJHIHIJIJIHHIHIIJJJJJIHIJJJJJJJGIJJGHGHIDHIIIIIIGIEHDHFHGFFDFDC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:77:4177:11048/1	16	MtDNA	9303	60	101M	*	0	0	CGTTTAGTAATTAGAGATTATTATTCTAATAGAAGACCTGAGTATTGTATGAGTAATTATGTATTTGGTCACAGTTATCAGTCTGAGATTTATTTTAGAAC	DGEGEEGDEEDGFGEGGEGGGFFGDGGFFFFFFFFAGFDGGFGGDFGGGGGGGGGFGFGGGGGFDGGGGGEGGGGFGGGGGGGGGGGGGGFGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2202:8985:17141	147	MtDNA	9340	60	100M	=	9258	-182	CTGAGTATTGTATGAGTAATTATGTATTTGGTCACAGTTATCAGTCTGAGATTTATTTTAGAACTACTAGATTAAAAAATTAGAAACCTTAGTATAATTT	CDDEEEEEFFFFFFFHHHHHHJIHDJJIJJJJIHJJJJJJJJJJJIJJJJJJJJJIJJJJIIIJIHJJJJJJJJJJJJJIIJJJIHJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1305:20758:28262	99	MtDNA	9506	60	100M	=	9991	585	GTTCATACCCTTGAGGTGGTTTTCTCTTATTAAAAGTTTTAGTATAAAAAAGTATATTTTATTGTCAATAAAAAGGTAAAAACTTTAGATTCTTTAGTAT	CCCFFFFFHHHHHFHJAEHCDHIGJJIJHIJGIHIJFHIJHJGIIJDGHJJGBFHIJJJIGHIGIIIHBDHGGIEACHHFFFFFEEEEEEEDCEDDCCA;	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:76:8573:4029/1	0	MtDNA	9566	60	101M	*	0	0	ATTGTCAATAAAAAGGTAAAAACTTTAGATTCTTTAGTATAATTCAGTATGTTTGACTTCCAATCAAAAGGTTTTGAGGATTAATTAATAATTTTTTTCAA	DDDDDDD=DDDAB>CD?DDDDDDDDDDBDDDDDDDDDBDDCDDDDDDBDDDBDDDDDADDDDCCDBB=BDDBDDDD?DDBBCCCCCCDDBCBCDBDBCCCB	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:59:16574:9800/1	0	MtDNA	9610	60	101M	*	0	0	CAGTATGTTTGACTTCCAATCAAAAGGTTTTGAGGATTAATTAATAATTTTTTTCAAGGATATAATTTACTATTTCAGCATAGTTTATTTGCTAGTTATAT	GFAFGFDFFEFFFDDFGGGGGFGCGED=EEDDDDDEGGCGEGGGGGDFGDEFFCE?EGFEGGGDGGFGBGGCGEDGGFG?FDFBFFDGC=AEEAEDDFFDD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:39:12621:19315/1	0	MtDNA	9636	60	101M	*	0	0	GTTTTGAGGATTAATTAATAATTTTTTTCAAGGATATAATTTACTATTTCAGCATAGTTTATTTGCTAGTTATATAGATTGATTTCATAGGTTTAATTGTA	DBEEEEEEEB?E:EEFFFFB:CDCEFFACFAEEBEBCB?CFEFEFEFFFFFFFDBAC@BC=C;?<EBBEEBDCBBDFDEEB5ACC??B:BE@E?@@A@AA:	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:97:3517:19360/1	16	MtDNA	9687	60	101M	*	0	0	GCATAGTTTATTTGCTAGTTATATAGATTGATTTCATAGGTTTAATTGTAGTTTATTGTTGGGAGTTTTAGTGTTTGTTACTTTATTGTTTGGATATTTAA	=DF=DEGGGGFGDGFGFF?FGGEGFG?GGGFFGGGGDBGGGFFFEGGGGGGGGGGGFGEEGGFGGGGFGGGGGGGFGGGEGGGGGGGGGFFGGAGGGGGGF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:113:4491:15082/1	16	MtDNA	9710	60	101M	*	0	0	TAGATTGATTTCATAGGTTTAATTGTAGTTTATTGTTGGGAGTTTTAGTGTTTGTTACTTTATTGTTTGGATATTTAATTTTTGGTACTTTTTATTTTAAA	=BEE?EEE=EEA=5EEEEEEEAEEEEDD;EE;AEE?=EEDDCE=EED?BEEEEE?EEEBDEEEE?A=DEE6EEE=EEEAEEDDEEE?@EEEGFGDGEFF:F	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:73:1194:5250/1	16	MtDNA	9720	60	101M	*	0	0	TCATAGGTTTAATTGTAGTTTATTGTTGGGAGTTTTAGTGTTTGTTACTTTATTGTTTGGATATTTAATTTTTGGTACTTTTTATTTTAAAAGAAAAAAAA	FF=FBFEEFFFEFFFDFDFCFEEFFDEFFFBFFAFFDFFFFFFFFFEEFFFFFEFFFFFBFFFDFFDFFFDEEEEEEECDEFFFFDFDFFFFFFFFAFFDD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:24:12750:7270/1	16	MtDNA	9752	60	1I5M1D95M	*	0	0	GATTTAACTTTGTTACTTTATTGTTTGGATATTTAATTTTTGGTACTTTTTATTTTAAAAGAAAAAAAATTGAGTATCAGTTTGGTGAATTATTGTGTAGT	=5EAEE@EEE?FFFAFED:DBEGDEEGDFFGBFEGGFBFFFEED?>C:E8FG:GDCGGG<GF<GAGBGGDGGDGGGD=GGFFGGGFGGGGGGEBGGGGDGG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:1:39:6442:9317/1	16	MtDNA	9797	60	101M	*	0	0	CTTTTTATTTTAAAAGAAAAAAAATTGAGTATCAGTTTGGTGAATTATTGTGTAGTATTTTTCCAACTATTATTTTATTAATACAAATAGTTCCTTCACTT	D8@=CADGEBDGGEEEGGFFEEEFAFG=EFEGGFFDGGGGDGGGGGGEAGGGFGGGFFGGGFFFEDBEGEGGGEGGGFGGDGDGGGEGFGGEGGGGFDFFF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1305:20758:28262	147	MtDNA	9991	60	100M	=	9506	-585	GATATTCCTGGGTTAGAATTTGACTCTTATATAAAATCACTAGATCAATTAAGTTTAGGTGAACCACGTTTATTAGAAGTTGATAATCGTTGTGTTATTC	DEDDDDBBB?;DFFFFFFFEHHEGHIHEGIJJJJIIGHHIGIJIGIJJIGJJJJGHJJIIHHBIGFGBJJJIHEIFJJJJJJJIJJIHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2105:2690:5126	99	MtDNA	10115	60	100M	=	10123	108	GTATTACATCTGCTGATGTTATTCATGCTTGGGCATTAAATTCTTTATCTGTAAAATTAGATGCTATAAGAGGAATTTTAAGAACATTTAGTTACAGGTT	C@@FFFFFHHHFHJJJJJJIIJJ>FHIIJJEHIIJIJJJJJJIJIJJJJJIFGGIIIJIIIJIIJJIJJIHHGGIFHIIIIHCAEHFHEFFEFFEDEC>@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2105:2690:5126	147	MtDNA	10123	60	100M	=	10115	-108	TCTGCTGATGTTATTCATGCTTGGGCATTAAATTCTTTATCTGTAAAATTAGATGCTATAAGAGGAATTTTAAGAACATTTAGTTACAGGTTTCCTATAG	DEEDEDFFFFFFHHGFGHJGGIJHGDJIJJJJIGJJJIIIJHIIJJJJIGJJJIJJJJJJJIJJJJJIJJIJJJIHJJIJJJJJJJJHHHFBFFFFFCC@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:95:13542:11427/1	0	MtDNA	10154	60	101M	*	0	0	ATTCTTTATCTGTAAAATTAGATGCTATAAGAGGAATTTTAAGAACATTTAGTTACAGGTTTCCTATAGTGGGAGTTTTTTATGGTCAATGTTCAGAGATT	GGGGDFGFDGEGGGGGFDGGGGGGGGEFGDGGDGFGEGFGFGGGGGGGDGFFEEGEFGBCGGGGGEGFBEEEECEBEEFFGE=EGEECDEEEEGGDCEDDB	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:56:15270:6191/1	16	MtDNA	10392	60	101M	*	0	0	ATATAGAGCTTTAAATTTTACTTGTTTAAATATTGGTATTGCATACTATTACAATAAAATTTCATGTTAATGAAAAATAGAAACAAAGGGTAGAGTAAATA	GEEEEGEEEEEDBGEFEGEEGGFEGGFGGEEFEGGGEGFGGDEGEGGEGCDFGGGGGGGGGGGGGGFAGGGGGGGGGGGGGGGGFDGGGGGGGEGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:51:14694:9582/1	16	MtDNA	10415	60	101M	*	0	0	GTTTAAATATTGGTATTGCATACTATTACAATAAAATTTCATGTTAATGAAAAATAGAAACAAAGGGTAGAGTAAATATTAGTTTTATTGTTTCATACTAA	DEDEFEFEDAEEFF?EFFFEEBEAEE=ECCDDFFFFFFFFEFFEFFD=E@DFFFFFFFFFFFDBFFFEAFEFDFFFDEFFFEGGEGGFEGGGGGFFDFDDE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:69:16487:9482/1	0	MtDNA	10798	60	101M	*	0	0	TAGGCTTTATATTAAAGCTGGCTTCTGCCCTATGATATTTAAATGGCAGTCTTAGCGTGAGGACATTAAGGTAGCAAAATAATTTGTGCTTTTATTGAGTT	GGGGGGEGGGDEGFGGGGGEGGFGGGGGGGGGGFGDGFGGDGGGEGGFGEGGGGDFEDFBFDEEEEGEFFGCDDFEEGEEEE=GGB@FFDFDGAGAA5EC5	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:65:19066:9962/1	16	MtDNA	10886	60	101M	*	0	0	CTTTTATTGAGTTCCAGTATGAATGAAGTTATTGGTTAGTTCTATTTATGTTTTATGTTTGAATTTAATTTTTATTTAAGAAAAAATAAATATATTTATAC	E=@BBD@BEEEE@EE@EEEEEEEBEEBDDDE?FEEBEFFFDDBEBFEEFEDEEEFEBBEAEEFDADFF8CCEEE=??CCCCAFFBFCBEDCB=DEEEEEEE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:57:11714:20002/1	0	MtDNA	11269	60	101M	*	0	0	TTTATCTTGATAAATATTTATATTTAATACATTTAGTACGAAAGGAACATTGTAAAAGTTTTAAACTTTAAAGATTTTGAAATCTTTATTTTAGTGCTATT	FFFFFEFFFAFFF?FFFFFFFFFFFDFFFFFEFFFFEFFFFDFFBDEFFDFDFFFFFFFFFFFEBFFFFDF=FFFFFFCEEEEFEFFFEFEEE=@BECBBD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:39:7418:8959/1	0	MtDNA	11394	60	101M	*	0	0	TTTATTATTTGCTTTTTATTTGATTAATTTTTTATTAAGAATTAAGGATATAGGAAAAAATAAAATTAGAGCGTTTGAATGTGGTTTTGTAAGAGTTGGAA	GGGDGG?GGFFGGEGFFDFEFFFAFFFEFFFFFEFFFFFDEFFFDFFFF:E?EEEFACFFFFEEEFFFFAFFBFBFFBFFEBEC=ECEEBBBA=D=?CDAF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:41:12959:4517/1	16	MtDNA	11405	60	101M	*	0	0	CTTTTTATTTGATTAATTTTTTATTAAGAATTAAGGATATAGGAAAAAATAAAATTAGAGCGTTTGAATGTGGTTTTGTAAGAGTTGGAAAAATATGATTT	EGGEFGGEBGFDGGGGG@GFGGGFEGGGFGGGDGGGGGGDFGGGGGFEGBGFFFD?GFGFEGGGEGGGGEGGGGGGFGDGFGGGGGGGGEGGFGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:5
+EAGLE:2:94:12001:2695/1	0	MtDNA	11494	60	101M	*	0	0	AAAATTCAAAATTCTTTTAGAATTCATTTTTTTATTATGATATTGATATTTGTTATTTTTGATTTAGAAATTGTTATGTTTTTAGGTATTTTAGTATCAGA	?EEDEAEEEEDFEFFDEFEDFFEAFDFFFFFFCFDFCFEFF?FFFFFFFAFFDFAFFDFFFFFDFFFDFFFFF=F?DFEFFFA@FF?EFFF?FFEFFF=?:	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:26:8245:3800/1	0	MtDNA	11527	60	101M	*	0	0	ATTATGATATTGATATTTGTTATTTTTGATTTAGAAATTGTTATGTTTTTAGGTATTTTAGTATCAGATTTAAGTTCGTATATCAGGTTTTTAATAATATT	:FGEEFGFFGGEGGGEFFFFDFFFFGFEGBFFEFEEEEEEDE?EEDEEEBAE?EAEEEECFEDFF=FBFEEA??EEEEEEFEEG?DFF?DEFF?AB;EDDD	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:70:14381:7815/1	0	MtDNA	11624	60	101M	*	0	0	TATTCATCTTCATCTTGGGAGGATTTTACATAGAGTGATGATATGGTAAATTAGTTTGAGTAATTTAATTAATATTTCTATTTTTTTGATTGGATTTGTTT	GGGGGEGGGGGGFGGEGGGGFGFGGGGAGGGFGGGEGFGFGFEGFGEG?GGGFGFFFFBF@EEDEGEEGBADGFGGGGGGGBGGGDGC@F?EECDFFFDAC	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:41:9505:15324/1	16	MtDNA	11679	60	101M	*	0	0	TTGAGTAATTTAATTAATATTTCTATTTTTTTGATTGGATTTGTTTTTTTTATAGGTGGAATTAGTGTTTGGCTTATACCCACATTTAAATTAGGAATCTT	EBE=E=EEEEEEEEEE=BEEDEEEEEDEEB?EEEEEEE>EEECCC;EEEEEEEDEEEEE5EDEEEEDDAEEEDDEEDADDD:EEDEDF?AFEBDEDDDBD?	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:1:16170:13381/1	0	MtDNA	11707	60	101M	*	0	0	TTTTGATTGGATTTGTTTTTTTTATAGGTGGAATTAGTGTTTGGCTTATACCCACATTTAAATTAGGAATCTTTTTTTTAGAATGAGATTTTTTAAGGTTA	FFFFFBFFFFFDFDFGEGGGDG>@GAED=EDCCDA:DADCE?EEEFD==FFFEDB?FFFFC:EC:ECCBBC?EEECAFC8=C:=D?E5ECFEFDB:E:CAE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:85:11274:20902/1	0	MtDNA	11790	60	101M	*	0	0	TGAGATTTTTTAAGGTTAAAATTTAATTTTTATTTTAATAGAATCTTATTTTCGTTTATTCTTTTTTTGGTAACGTTTAGAGTTTTAGTTTTTAGTAGTTA	GGGGFGGGGGGGGGGDGGGFGGGGGFGGFGGGGGGGGGGGGEGGGGFEGGGGGGGGGEGGGGGGGGGGEFCEFFGFFEEEEGEGGGCFDEGFEEF=:6??:	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+EAGLE:1:45:6186:13482/1	0	MtDNA	11795	60	101M	*	0	0	TTTTTTAAGGTTAAAATTTAATTTTTATTTTAATAGAATCTTATTTTCGTTTATTCTTTTTTTGGTAACGTTTAGAGTTTTAGTTTTTAGTACTTATTATT	DFFFEBAEEEDDADDEDED==CCCCE=EEEEEEDBEEE?EEDDEEAB@EEDDEEDEEAEECEECA>:ACC@DCCB?DAABEBEBCEEEB?EBBEEB=E@EE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:26:5376:8009/1	0	MtDNA	11861	60	101M	*	0	0	AACGTTTAGAGTTTTAGTTTTTAGTACTTATTATTTAAATAGTGAGTTAAACTTTAATTATTATTATTTTGTATTGTTAATTTTCGTAGGTAGAATGTTTA	ADC<DCEDBEAF?D:DBEEAFBBDBAE?EEDG?GEEGEEFEE??ED:AD?EEEEEBBGGD?FADFDGGGFEBDEDDEAEEDGFGBE5CEE=DD=?:B>ABB	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1301:9999:63927	99	MtDNA	11891	60	100M	=	11969	178	TTATTTAAATAGTGAGTTAAACTTTAATTATTATTATTTTGTATTGTTAATTTTCGTAGGTAGAATGTTTAGGCTAAATTTTAGAAACAGTATTTTTACA	CCCFFFFFHHHHFHIJJJIJGIJJJJIJJJJJJJJJJJJJJIJJJJIJJJJJJJJJHIJIFGIIJJJIJJJIJIJHGIJJJJJIIHHHHHADFFFFFDCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2202:18436:71346	99	MtDNA	11949	60	100M	=	12056	207	GGTAGAATGTTTAGGCTAAATTTTAGAAACAGTATTTTTACAATGTTACTAAGATGAGATTTATTGGGTATTTCTAGGTTTTTTTTAGTTTTATTTTATA	BCBDFFFFHHHHHJJJJJIJJJJJIIIJJJJJCHIIJJJIIIJJJGIIJJJJIIJIGIFHHIHIJIJIFHHJJJJEIJEHIJHFDDDDDDEDDDDEEEDD	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:74:13913:12442/1	16	MtDNA	11959	60	101M	*	0	0	TTAGGCTAAATTTTAGAAACAGTATTTTTACAATGTTACTAAGATGAGATTTATTGGGTATTTCTAGGTTTTTTTTAGTTTTATTTTATAATAATTGAGAT	GGGEEEFFBEEDGFGGFEDGFGGFGDGGGEGGGGGGGEGGFGFGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGFGGCAAA@D9EEEGGGGGGGGGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1301:9999:63927	147	MtDNA	11969	60	100M	=	11891	-178	TTTTAGAAACAGTATTTTTACAATGTTACTAAGATGAGATTTATTGGGTATTTCTAGGTTTTTTTTAGTTTTATTTTATAATAATTGAGATAGATGTAGG	ADEEDDDCCEDDDDDDEEEEDEEEFFFFFFFHHHHHHJJJJJJJJJJJIIJJJIJJJJIIIJJJJJJIJJJJJJJJJJJIIIJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:27:3003:10682/1	16	MtDNA	12043	60	101M	*	0	0	TTTATAATAATTGAGATAGATGTAGGGGTGCAATAAATACAGCATTAACTAATCGTCTAGGTGATTATTTTATATTTGTCTTTTTTGGTTTGTCGGTTTTT	FB?CEACEE=EDEEE==?EE=DFFEAEEEE;FCEFEBAF?F?FFDFBEDGGFBEGGGGFGGGGEGFF?;FFFFDDDGDEGEFGEGGGGDAFGGGFGDFGGB	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:2202:18436:71346	147	MtDNA	12056	60	100M	=	11949	-207	AGATAGATGTAGGGGTGCAATAAATACAGCATTAACTAATCGTCTAGGTGATTATTTTATATTTGTCTTTTTTGGTTTATCGGTTTTTAGAGGTTATTAT	DDEDDDDDDDDDDDDDDDDFEFEDCDDDDDEEDCCEDFFFHHHHJJJJJJJJJJJJJIJJIIJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1116:18352:37917	99	MtDNA	12193	60	100M	=	12359	266	TATTATTACTTTTAACAGCTTTTACTAAAAGAGCACAATTTCCATTTAGATCTTGGTTACCCAAAGCTATAAGAGCCCCCACACCGGTGAGGTCTTTGGT	@@@FFFDDHGHHHII@FGGGGGGIIIIIIJJIIGGIIJIJFHIGGIJFEHDBGHHGBGIJIHGIAEHIIIJCG@ACCDHEB<?A=>@,8<995<CCDD?B	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2313:14435:43021	99	MtDNA	12276	60	100M	=	12492	316	CCGGTGAGGTCTTTGGTTCATAGTAGAACTTTAGTTACAGCAGGATTAATTCTATTAATAAATTTTAATAATTTAGTAATACAGAAAGATTTTATCAGTT	B@@DADBDFCFHHIGGIIJGIJJJJJJJJJJJGIHGIGHJJIIJJJGHIIFFFICIIJIJICHJGGGFHHIIJIIIHIIJIHGGEHGF@BCFFECCDE@@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1116:18352:37917	147	MtDNA	12359	60	100M	=	12193	-266	GAAAGATTTTATCAGTTTTGTTCTGATTATTGGCCTATTTACTATATTTTTTTCTAGCTTAGCAAGTTTGGTCGAAGAAGATTTGAAGAAGGTGGTAGCC	DDDDCEDDDCCADCDDDDCAC>CACA@C>FFFFHGHHHEE@>CDAHFEGIEIJIGIGHIIGIGHGJIIIHIGIHIJGGHDJJIIHEIHHHHHFDFDF@@@	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:4:6493:13587/1	0	MtDNA	12373	60	101M	*	0	0	GTTTTGTTCTGATTATTGGCCTATTTACTATATTTTTTTCTAGCTTAGCAAGTTTGGTCGAAGAAGATTTGAAGAAGGTGGTAGCCTTGAGGACACTTTCA	B88:7D??>BGFGDFEDDEFEDBEEEGEEBE=DEEEEEEEEEEGED@EDE5BABBEE>AAA?@?@@?5??BB?BBABA>B?7:??AAABAAAA?A<:>A>?	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:1:5:16925:17939/1	0	MtDNA	12403	60	101M	*	0	0	TATTTTTTTCTAGCTTAGCACGTTTGGTCGAAGAAGATTTGAAGAAGGTGGTAGCCTTGAGGACACTTTCACAAATAGGTTTTTCAATAGTTACTTTGGGC	FFF5FFEEECGGGEGEDEEE=EEEEEEEEEEEDEEEEEEEEEEEEEEDDEEE=E?BEEEEEEDEEEEEEEEEEEE6CCB>AEEEEEE?BADEEBEEAE=EE	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+EAGLE:1:79:6338:4546/1	0	MtDNA	12420	60	101M	*	0	0	GCAAGTTTGGTCGAAGAAGATTTGAAGAAGGTGGTAGCCTTGAGGACACTTTCACAAATAGGTTTTTCAATAGTTACTTTGGGCCTAGGGCTTAGGTTTAT	FFDFFFFFFDEFBFFGGGGGFGEEGGEFFFECEECFFFFFGGGGFGGGGGGGGGFGEFEGGGEGEFFF?DFDFCFEEFEFEBFGEEEEBGEEEEDGEEEEF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1313:2251:84798	99	MtDNA	12449	60	100M	=	12482	133	GGTGGTAGCCTTGAGGACACTTTCACAAATAGGTTTTTCAATAGTTACTTTGGGCCTAGGGCTTAGGTTTATTTCATTTATTCATTTAGTTAGACATGCT	BCBDFDFFHHHHHJIJIJJJJJJJJJJJIJJIJEFHJJJGIGHJIIJJIJJJIIGHJIIIJJJJJJIEHHIGHHHFHHHFFFFFFFFEEEEEEEDCDDDC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1313:2251:84798	147	MtDNA	12482	60	100M	=	12449	-133	TTTTTCAATAGTTACTTTGGGCCTAGGGCTTAGGTTTATTTCATTTATTCATTTAGTTAGACATGCTTTGTTCAAAAGATGTTTATTTATACAAGTAGGT	ADDDDDEECEC>CDFFFDHHHHIJJJJJIIJJJJJJJJJJJIJJJJJJJJJJJJIJJIJIIJJJIJJJIIGIJJJJJJJJJJJJJJJHHGHGFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2313:14435:43021	147	MtDNA	12492	60	100M	=	12276	-316	GTTACTTTGGGCCTAGGGCTTAGGTTTATTTCATTTAATCATTTAGTTAGACATGCTTTGTTCAAAAGATGTTTATTTATACAAGTAGGTTACATTATTC	ACC@CEDCDBDDFFHHHFIGHGIIGIJGIIIJHGHD?4JJJJIHIJJIGIHJIJJJJJIJIIJJJJIJJJJJJJJJJJJIHJJJIJJHHHHHFFFFFC@C	PG:Z:SNAP	RG:Z:000	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:1303:13602:38947	99	MtDNA	12495	60	100M	=	12781	386	ACTTTGGGCCTAGGGCTTAGGTTTATTTCATTTATTCATTTAGTTAGACATGCTTTGTTCAAAAGATGTTTATTTATACAAGTAGGTTACATTATTCATT	?????DD::C:2<3C?;EECFFEIEFIEDE9?@E@DA<DDCDCDEEDAECDDDDA@DEDCEAC>C;CA7=ACCEDCEIEED>)=?A7?7?DDD@>BD@AD	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:102:10210:8668/1	0	MtDNA	12538	60	101M	*	0	0	TTAGACATGCTTTGTTCAAAAGATGTTTATTTATACAAGTAGGTTACATTATTCATTGTTCATTTGGACAACAAGATGGACGTAATTATAGGAATAATGGT	GFGGGGGGGG:FF?FGGGFGGGFGEBFGGGGFFDDGGGGGGGGAGDEEEEGFFBGGDED;EAGGGDGEEG?FDFFBEG5EEDCEEFDA5FEEBEF?EEB::	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2214:8352:14578	99	MtDNA	12620	60	100M	=	12676	156	TAAGTATAGGAATAATGTTAATTTACCAAATTTTATTCAGTTACAAATATTAGTAACCCTATTTTGTTTATGTGGATTAATTTTCTCAAGGGGTGCTGTA	@CC1BDFFHHHHHJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJIJJJJJJJJJIJJJJJJJJJJJJJJIGHJGIIIGHIFGIJJJJIGHHHFDBABCDDD	PG:Z:SNAP	RG:Z:000	NM:i:2
+BS-DSFCONTROL03:121:D0M8KACXX:5:2214:8352:14578	147	MtDNA	12676	60	100M	=	12620	-156	CCCTATTTTGTTTATGTGGATTAATTTTCTCAAGGGGTGCTGTAAGAAAAGATTTTATTTTAGAATTATTTTTTTCTAATAACTATATAATGTTTTTTAG	?CCDDBDDDDDDDDDDDDDEDDEDEEEAFFFFFHHIIEJJJGIJJJJIHFGJJIJJIIGIJJJJIIJJJIJJJJJJJJJIJJJJJJJHHHHHFFFFFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:101:7524:6107/1	0	MtDNA	12685	60	101M	*	0	0	GTTTATGTGGATTAATTTTCTCAAGGGGTGCTGTAAGAAAAGATTTTATTTTAGAATTATTTTTTTCTAATAACTATATAATGTTTTTTAGATTAATATTT	BEEEEDD5DDEEEDEFEDFFAFBDFFFFDFFFFAFEED=E:CBEEFFEDFFEEFEDFFFFDFFFFDF@EFBDFFBEFEFABCD:EFFCCCDD:EADFEFFF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:73:3706:15683/1	0	MtDNA	12685	60	101M	*	0	0	GTTTATGTGGATTAATTTTCTCAAGGGGTGCTGTAAGAAAAGATTTTATTTTAGAATTATTTTTTTCTAATAACTATATAATGTTTTTTAGATTAATATTT	DDDD:DDBDBBEEEEGFFFGGGFGGGFGDF?ADCEFF=EB?CE:BFF=FCCE>FDEDEAAGAGCCEEGEEGGFEFEFEGD@EFADEFCC>E?E=BBBAEBF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:35:14999:3587/1	0	MtDNA	12745	60	101M	*	0	0	TTTTTTCTAATAACTATATAATGTTTTTTAGATTAATATTTTTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTC	DEFFFFFFFFBFD?FFFBFFFFFAFEEBECFDFFFFFDAFFFFFFCFFFBECECEFFEC?FBFFFDDEE??CDBEFFFFEEFFFFABFFFE8CFFFFFFE;	PG:Z:SNAP	RG:Z:266-1	NM:i:1
+BS-DSFCONTROL03:121:D0M8KACXX:5:1101:18737:28666	99	MtDNA	12755	60	100M	=	12783	128	TAACTATATAATGTTTTTTAGATTAATATTTTTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTAAGGTTTAAT	CCCFFFFEHHHHHJJJJJJGJGHJJJIGIIJJJJJHIJIIIGHIJJJJJHIIJJJJJIJJJJJIGHHHHFFFFEECECEDDBDDDDDDDDBCCDCCDDDA	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:1:53:16100:4061/1	0	MtDNA	12755	60	101M	*	0	0	TAACTATATAATGTTTTTTAGATTAATATTTTTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTAAGGTTTAATA	FFDFFDEFDFAFE=FDFE@AFB?DFFF5FF?D@<@ACAEEE:ECEEEAA=FFDFDFADDFEEE@EEEEE@AABBB=AEEEDCCFDBEFE@@@DBADEDBBF	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1303:13602:38947	147	MtDNA	12781	60	100M	=	12495	-386	TATTTTTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTAAGGTTTAATAAAGTAATAAATCATTACAGTAGCAC	:?BBBB?CC@5CCCA9'=>>5(;>C;;A>>?;B@BFEFHEBG@=@>FB;BCGB?<GDEDC9<F<EEA<4D>HEGJHE<C<?<?A<2,,?B=2A4DDD8@?	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1101:18737:28666	147	MtDNA	12783	60	100M	=	12755	-128	TTTTTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTAAGGTTTAATAAAGTAATAAATCATTACAGTAGCACAG	BDBDDDDCCDDDDBBDDDDEDCDDDDEEEFFFFFFHHFIJJJJJJJJIIHJIIJJJJJJJHGJJJJIJIJJIJJJJIJJJJJJJJJJGHHHHFFFDFCCC	PG:Z:SNAP	RG:Z:000	NM:i:0
+EAGLE:2:109:6839:9395/1	16	MtDNA	12786	60	101M	*	0	0	TTTGTTTCAGTGTTTTTGACTTTTGGTTACAGTTTTCGTCTTTGAAAAAGATTTTTTTTAAGGTTTAATAAAGTAATAAATCATTACAGTAGCACAGTATT	GGGGGFEFFGGGGGGDGGDGFDGGGGGGFGGGGGGFFGGGFGGGGGGGGGGGGGGGGGGFBGGGGGDGGGGGGGGGGGGGGFGGGGGGGGEGGFGGGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:89:16184:15896/1	16	MtDNA	12850	60	101M	*	0	0	TTAATAAAGTAATAAATCATTACAGTAGCACAGTATTTATAAATTTTTTAAGTTTAGTATTAGTTATTTTTTCTATTAGATTTTTATGATGAATAAATTTT	EEEEFEEDFEEEGEBEEGEEBEGEEFGEGEGDGBFGFGGFGDFGEGGFEGGGFGEGGGGGFFGCEGGGGGGDGFFGDEGDGGGEGFDGGGGEGFGFGGGGG	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+EAGLE:2:73:18789:5004/1	0	MtDNA	12862	60	97M1I3M	*	0	0	TAAATCATTACAGTAGCACAGTATTTATAAATTTTTTAAGTTTAGTATTAGTTATTTTTTCTATTAGATTTTTATGATGAATAAATTTTAATCTAATAGAA	GFEGFGGF=FEADEEEEEEDEEDEEFFFFFGGGGGGDGGGEGDFABE:EEBA=BBFFCCCGE@FF?BBBCACCB8>BEEEFFFGGFFGGE?EEEBD5?BBE	PG:Z:SNAP	RG:Z:266-1	NM:i:4
+BS-DSFCONTROL03:121:D0M8KACXX:5:1114:11635:22812	99	MtDNA	12940	60	100M	=	13083	243	GAATAAATTTTAATCTTCTTAACATCCCAAGACTTTTCTTATACGTAGATTTTTTTGGTCCTCTAGTATTTTTATTTATAATAATTTTTTTATCTTTTTT	CCCFFFFFHHHHHJJJJIJJJJJJJIJJJJJJJJJJJJJJJJIIJHIJIJGHJJJJJIHHIJJJFHEHHHHHFFFFFFFEEFEEEFEDDDDDDDDDDDD<	PG:Z:SNAP	RG:Z:000	NM:i:1
+EAGLE:1:50:13913:15312/1	16	MtDNA	13005	60	101M	*	0	0	GTATTTTTATTTATAATAATTTTTTTATCTTTTTTAATTTTAAAAATATTATTTAAGGAGTTAATATACAAGTTTTTAGTTGATTATTTGGCTAAAAATAG	?>:>;::BCBB::FFF@@8<96BABC=@DFFBF=EDFDEFCAE=?EE?FAFEEC?=EEEC=FDEEBEEDAFF?EFFEBEDDEEEEBCDDD?E@<DDDEECE	PG:Z:SNAP	RG:Z:266-1	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:1114:11635:22812	147	MtDNA	13083	60	100M	=	12940	-243	GTTGATTATTTGGCTAAAAATAGTATTTATAAAATAAAGAATTTAAAATTTATAGATTTATTTTTAAATAATATTAATTCTAAGGGGTACACCTTATTTT	@CFEDEFFFHGHGIJJJJJJJIHEGJJIHCJJJJJJJJJJJJJJJJJJJJJJJIJJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHDHFFFFFCC@	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2115:12001:40456	99	MtDNA	13159	60	100M	=	13242	183	ATTCTAAGGGGTACACCTTATTTTTAAGCAGTGGTATATTTAAAAATTACTATTTAAAAAGGTTAAATTTTAATAGTGTAGTAGTTTTAATTTTTATTTT	@@@FFFFDFFHDFGGEB;AB<EHGGGBH@FH?CC?F@GGIII>DGHGEDBDHHEDHGHIIGCHGI@@FGHIHGGIC==EC>)=A?CD>DDDEEECAACDD	PG:Z:SNAP	RG:Z:000	NM:i:0
+BS-DSFCONTROL03:121:D0M8KACXX:5:2115:12001:40456	147	MtDNA	13242	60	100M	=	13159	-183	GTTTTAATTTTTATTTTTTTTATAATTTGTTAAGGGATTTTAGTTTAATAAAAATATATGTTTTGCATACATAAGATAATAATTCTAGATAGTTTTACAC	@DDDDDDDCACDDDFGJIIJIJIIG@HIIJIHIHJJIIGJJIHGGIEIIGIIIIJIGIJJJJIGGCIIIGJIJJJIJJJIHIIJJJJHHFDGFFDDD@@@	PG:Z:SNAP	RG:Z:000	NM:i:0
diff -r 000000000000 -r f0f2795de2c7 test-data/a.vcf
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/a.vcf	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,192 @@
+##fileformat=VCFv4.2
+##FILTER=<ID=PASS,Description="All filters passed">
+##reference=/home/wolma/galaxy_2017/database/files/000/dataset_35.dat
+##contig=<ID=chrI,length=25050>
+##contig=<ID=chrII,length=25050>
+##contig=<ID=chrIII,length=25050>
+##contig=<ID=chrIV,length=25050>
+##contig=<ID=chrV,length=25050>
+##contig=<ID=chrX,length=25050>
+##contig=<ID=MtDNA,length=13794>
+##ALT=<ID=X,Description="Represents allele(s) other than observed.">
+##INFO=<ID=INDEL,Number=0,Type=Flag,Description="Indicates that the variant is an INDEL.">
+##INFO=<ID=IDV,Number=1,Type=Integer,Description="Maximum number of reads supporting an indel">
+##INFO=<ID=IMF,Number=1,Type=Float,Description="Maximum fraction of reads supporting an indel">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Raw read depth">
+##INFO=<ID=VDB,Number=1,Type=Float,Description="Variant Distance Bias for filtering splice-site artefacts in RNA-seq data (bigger is better)",Version="3">
+##INFO=<ID=RPB,Number=1,Type=Float,Description="Mann-Whitney U test of Read Position Bias (bigger is better)">
+##INFO=<ID=MQB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality Bias (bigger is better)">
+##INFO=<ID=BQB,Number=1,Type=Float,Description="Mann-Whitney U test of Base Quality Bias (bigger is better)">
+##INFO=<ID=MQSB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality vs Strand Bias (bigger is better)">
+##INFO=<ID=SGB,Number=1,Type=Float,Description="Segregation based metric.">
+##INFO=<ID=MQ0F,Number=1,Type=Float,Description="Fraction of MQ0 reads (smaller is better)">
+##INFO=<ID=ICB,Number=1,Type=Float,Description="Inbreeding Coefficient Binomial test (bigger is better)">
+##INFO=<ID=HOB,Number=1,Type=Float,Description="Bias in the number of HOMs number (smaller is better)">
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes for each ALT allele, in the same order as listed">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=DP4,Number=4,Type=Integer,Description="Number of high-quality ref-forward , ref-reverse, alt-forward and alt-reverse bases">
+##INFO=<ID=MQ,Number=1,Type=Integer,Description="Average mapping quality">
+##FORMAT=<ID=PL,Number=G,Type=Integer,Description="List of Phred-scaled genotype likelihoods">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Number of high-quality bases">
+##FORMAT=<ID=DPR,Number=R,Type=Integer,Description="Number of high-quality bases observed for each allele">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Phred-scaled Genotype Quality">
+##source=MiModD varcall (version 0.1.8.0)
+##MiModDCommand=mimodd varcall /home/wolma/galaxy_2017/database/files/000/dataset_35.dat /home/wolma/galaxy_2017/database/files/000/dataset_54.dat --index-files /home/wolma/galaxy_2017/database/files/_metadata_files/000/metadata_14.dat --ofile /home/wolma/galaxy_2017/database/files/000/dataset_58.dat --depth 250 --verbose --quiet
+##samtoolsCommand=n.a. (wrapped by MiModD)
+##bcftools_callCommand=n.a. (wrapped by MiModD)
+##rginfo=<ID=0,Rgid="000",Name="N2">
+##rginfo=<ID=1,Rgid="266-1",Name="ot266",Description="mutant strain carrying the vab-3 allele ot266">
+##samtoolsVersion=1.2+htslib-1.2.1
+##bcftools_callVersion=1.2+htslib-1.2.1
+##bcftools_concatVersion=1.2+htslib-1.2.1
+##bcftools_viewVersion=1.2+htslib-1.2.1
+##bcftools_viewCommand=view /home/wolma/galaxy_2017/database/files/000/dataset_58.dat
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	N2	ot266
+chrI	855	.	G	C	231.0	.	DP=27;VDB=2.48853e-13;SGB=13.8822;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,11,13;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:255,72,0:24:0,24:86
+chrI	885	.	t	tTCT	69.0	.	INDEL;IDV=4;IMF=0.0769231;DP=56;VDB=2.27029e-09;SGB=1.27626;MQSB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=16,17,3,1;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,3,60:1:1,0:11	0/1:105,0,255:36:32,4:96
+chrI	912	.	A	T	195.0	.	DP=92;VDB=0.233506;SGB=-8.71232;RPB=0.810719;MQB=1;MQSB=1;BQB=0.974933;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=30,38,9,13;MQ=60	GT:PL:DP:DPR:GQ	0/1:26,0,54:2:1,1:25	0/1:204,0,255:88:67,21:127
+chrI	921	.	G	A	341.0	.	DP=101;VDB=0.0381167;SGB=14.497;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;AC=4;AN=4;DP4=0,1,41,52;MQ=60	GT:PL:DP:DPR:GQ	1/1:110,6,0:2:0,2:25	1/1:255,239,0:92:1,91:127
+chrI	942	.	A	T	182.0	.	DP=147;VDB=0.486211;SGB=-13.0084;RPB=0.177913;MQB=1;MQSB=1;BQB=0.00895523;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=42,63,15,21;MQ=60	GT:PL:DP:DPR:GQ	0/1:20,0,116:4:3,1:17	0/1:198,0,255:137:102,35:127
+chrI	945	.	G	A	396.0	.	DP=144;VDB=0.936097;SGB=17.553;RPB=0.198891;MQB=1;MQSB=1;BQB=0.921548;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=7,15,50,66;MQ=60	GT:PL:DP:DPR:GQ	1/1:177,12,0:4:0,4:19	0/1:255,0,86:134:22,112:78
+chrI	962	.	G	T	379.0	.	DP=141;VDB=0.0554366;SGB=20.8365;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,58,75;MQ=60	GT:PL:DP:DPR:GQ	1/1:148,12,0:4:0,4:36	1/1:255,255,0:129:0,129:127
+chrI	973	.	A	G,T	329.0	.	DP=140;VDB=0.0208629;SGB=18.7462;RPB=0.14071;MQB=1;MQSB=1;BQB=0.978744;MQ0F=0;ICB=0.3;HOB=0.125;AC=3,0;AN=4;DP4=7,12,44,71;MQ=60	GT:PL:DP:DPR:GQ	1/1:110,6,0,110,6,110:2:0,2,0:14	0/1:255,0,65,255,255,255:132:19,112,1:56
+chrI	974	.	A	T	9.21175	.	DP=139;VDB=0.000145084;SGB=10.9211;RPB=0.0841531;MQB=1;MQSB=1;BQB=0.939818;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=44,72,7,11;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,6,110:2:2,0:14	0/1:45,0,255:132:114,18:36
+chrI	998	.	A	T	99.0	.	DP=98;VDB=0.00196934;SGB=10.9211;RPB=0.976264;MQB=1;MQSB=1;BQB=0.993458;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=33,40,5,13;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,6,110:2:2,0:12	0/1:134,0,255:89:71,18:127
+chrI	1000	.	A	T	93.0	.	DP=108;VDB=0.000325892;SGB=11.6141;RPB=0.945574;MQB=1;MQSB=1;BQB=0.923217;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=34,48,9,10;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,6,110:2:2,0:13	0/1:128,0,255:99:80,19:120
+chrI	1028	.	G	A	105.0	.	DP=63;VDB=0.000108954;SGB=6.07412;RPB=0.732968;MQB=1;MQSB=1;BQB=0.91987;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=18,30,6,5;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,6,110:2:2,0:12	0/1:140,0,255:57:46,11:127
+chrI	1041	.	C	T	6.52941	.	DP=40;VDB=0.00951951;SGB=1.94933;RPB=0.416648;MQB=1;MQSB=1;BQB=0.652425;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=13,21,3,2;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,6,71:2:2,0:14	0/1:42,0,255:37:32,5:33
+chrI	1045	.	T	A	273.0	.	DP=36;VDB=1.8168e-09;SGB=2.16465;RPB=0.578971;MQB=1;MQSB=1;BQB=0.637911;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=4,1,11,18;MQ=60	GT:PL:DP:DPR:GQ	1/1:54,6,0:2:0,2:13	0/1:255,0,51:32:5,27:43
+chrI	1048	.	G	A	266.0	.	DP=30;VDB=1.83438e-10;SGB=2.65329;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,10,18;MQ=60	GT:PL:DP:DPR:GQ	1/1:35,3,0:1:0,1:20	1/1:255,81,0:27:0,27:98
+chrI	1053	.	c	cT	8.64418	.	INDEL;IDV=1;IMF=0.047619;DP=21;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:32,3,0:1:0,1:16
+chrI	1054	.	A	T	231.0	.	DP=18;VDB=4.22339e-07;SGB=7.05893;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,5,9;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:255,42,0:14:0,14:54
+chrI	1412	.	T	C	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrI	1414	.	G	A	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrI	1421	.	G	A	10.6576	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:35,3,0:1:0,1:4
+chrI	2752	.	C	T	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrI	13851	.	T	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrI	13856	.	T	A	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrI	13874	.	A	G	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrI	13876	.	C	T	10.6576	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:35,3,0:1:0,1:4
+chrI	13901	.	C	A	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrI	13919	.	C	A	9.75336	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:34,3,0:1:0,1:4
+chrI	13921	.	T	C	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	13944	.	T	A	11.8912	.	DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:6
+chrI	13952	.	G	T	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrI	13958	.	C	T	8.01478	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:32,3,0:1:0,1:4
+chrI	13970	.	C	T	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	13993	.	A	G	10.6576	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:35,3,0:1:0,1:4
+chrI	14201	.	G	A	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	14223	.	A	G	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	14242	.	T	C	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	14246	.	C	T	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	14247	.	T	C	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	22325	.	T	C	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	23943	.	g	gG	41.4034	.	INDEL;IDV=2;IMF=1;DP=2;VDB=0.94;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:66,6,0:2:0,2:9
+chrII	239	.	A	G	9.75336	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:34,3,0:1:0,1:4
+chrII	252	.	A	T	44.4523	.	DP=11;VDB=0.0411132;SGB=0.620439;RPB=0.916482;MQB=0.916482;MQSB=1.00775;BQB=0.366593;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,3,2,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:75,0,160:10:7,3:74
+chrII	265	.	A	G	161.0	.	DP=14;VDB=0.00228484;SGB=4.69269;RPB=0.965069;MQB=1;MQSB=0.966012;BQB=0.686908;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,1,3,6;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:191,0,96:14:5,9:96
+chrII	278	.	A	T	167.0	.	DP=14;VDB=0.000574122;SGB=4.69269;RPB=0.249352;MQB=1;MQSB=0.966012;BQB=0.867428;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=2,3,5,4;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:197,0,111:14:5,9:111
+chrII	279	.	A	T,G	38.2143	.	DP=14;VDB=0.0501588;SGB=1.94933;RPB=0.448332;MQB=1;MQSB=0.966012;BQB=0.118179;MQ0F=0;ICB=1;HOB=0.5;AC=1,0;AN=2;DP4=7,2,0,5;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0,0,0,0:0:0,0,0:0	0/1:69,0,186,71,175,247:14:9,4,1:67
+chrII	291	.	A	G	86.0	.	DP=14;VDB=0.0340507;SGB=1.94933;RPB=1;MQB=1;MQSB=0.966012;BQB=0.197899;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=6,3,1,4;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:116,0,189:14:9,5:116
+chrII	300	.	A	C	231.0	.	DP=14;VDB=3.24567e-05;SGB=7.05893;MQSB=0.966012;MQ0F=0;AC=2;AN=2;DP4=0,0,7,7;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:255,42,0:14:0,14:53
+chrII	304	.	A	T	137.0	.	DP=14;VDB=0.000156924;SGB=4.00342;RPB=0.991701;MQB=1;MQSB=0.966012;BQB=0.703221;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,5,6,2;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:167,0,107:14:6,8:108
+chrII	305	.	A	T	27.2811	.	DP=14;VDB=0.0785113;SGB=0.620439;RPB=0.973096;MQB=1;MQSB=0.966012;BQB=0.738577;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=6,5,1,2;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:59,0,236:14:11,3:56
+chrII	317	.	A	G	101.0	.	DP=14;VDB=0.00324086;SGB=2.63044;RPB=0.94925;MQB=1;MQSB=0.966012;BQB=0.997919;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,4,3,3;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:131,0,172:14:8,6:127
+chrII	331	.	A	T	121.0	.	DP=14;VDB=0.0126037;SGB=3.31571;RPB=0.497296;MQB=0.966012;MQSB=0.966012;BQB=0.834543;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=5,2,2,5;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:151,0,154:14:7,7:127
+chrII	343	.	A	G	90.0	.	DP=9;VDB=0.00476095;SGB=1.97062;RPB=0.346719;MQB=0.924584;MQSB=0.924584;BQB=0.462292;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,2,5,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:120,0,74:9:3,6:74
+chrII	11921	.	A	T	7.18923	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:31,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
+chrII	17381	.	G	A	4.14838	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:32,0,34:2:1,1:32	./.:0,0,0:0:0,0:0
+chrII	17518	.	A	T	14.9987	.	DP=3;VDB=0.5;SGB=0.0985265;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,2,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:45,0,29:3:1,2:30	./.:0,0,0:0:0,0:0
+chrII	19959	.	T	A	7.18923	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:31,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
+chrIII	7994	.	C	T	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrIII	8004	.	T	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrIII	8023	.	A	G	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrIII	8026	.	A	G	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrIII	8027	.	A	G	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrIII	8039	.	C	A	9.75336	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:34,3,0:1:0,1:4
+chrIII	9659	.	C	T	9.75336	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:34,3,0:1:0,1:4
+chrIII	9662	.	G	T	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrIII	9700	.	A	T	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrIV	9450	.	A	G	4.78389	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:33,0,27:2:1,1:28	./.:0,0,0:0:0,0:0
+chrIV	16467	.	T	C	4.14182	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:32,0,25:2:1,1:26	./.:0,0,0:0:0,0:0
+chrIV	16478	.	C	G	4.58205	.	DP=3;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=0,2,1,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:35,0,23:2:1,1:28	0/0:0,3,29:1:1,0:5
+chrIV	16499	.	A	G	3.42278	.	DP=3;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=1,1,0,1;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,6,79:2:2,0:4	0/1:35,3,0:1:0,1:3
+chrIV	16537	.	aaga	a	25.8002	.	INDEL;IDV=1;IMF=1;DP=3;VDB=0.206307;SGB=-0.516033;MQSB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=1,1,0,1;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,6,120:2:2,0:4	0/1:60,3,0:1:0,1:3
+chrIV	16633	.	C	A	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrIV	16697	.	C	T	52.0	.	DP=2;VDB=0.48;SGB=-0.759771;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:40,3,0:1:0,1:9	1/1:36,3,0:1:0,1:9
+chrIV	16709	.	C	G	5.30774	.	DP=3;VDB=0.98;SGB=0.0985265;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=0,1,2,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:0,3,39:1:1,0:3	1/1:38,6,0:2:0,2:4
+chrIV	16729	.	C	T	4.14943	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=0,1,1,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:0,3,37:1:1,0:3	0/1:35,3,0:1:0,1:3
+chrIV	16769	.	A	T	13.7861	.	DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:6
+chrIV	16814	.	A	G	3.03341	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:30,0,31:2:1,1:30
+chrIV	16815	.	C	A	48.4033	.	DP=2;VDB=0.42;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:73,6,0:2:0,2:9
+chrIV	16823	.	A	C	4.14995	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:32,0,28:2:1,1:29
+chrIV	16829	.	T	C	3.56356	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:31,0,32:2:1,1:31
+chrIV	16833	.	A	T,G	14.5409	.	DP=2;VDB=0.42;SGB=0.0985265;MQSB=1;MQ0F=0;AC=1,1;AN=2;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0,0,0,0:0:0,0,0:0	1/2:65,34,31,34,0,31:2:0,1,1:29
+chrIV	16834	.	T	A	3.56452	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:31,0,27:2:1,1:28
+chrIV	16894	.	T	C	55.0	.	DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	1/1:41,3,0:1:0,1:9	1/1:38,3,0:1:0,1:9
+chrIV	16899	.	T	A	49.5769	.	DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	1/1:41,3,0:1:0,1:9	1/1:33,3,0:1:0,1:9
+chrIV	16902	.	T	G	55.0	.	DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	1/1:41,3,0:1:0,1:9	1/1:38,3,0:1:0,1:9
+chrIV	16921	.	A	G	68.0	.	DP=3;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	1/1:54,3,0:1:0,1:11	1/1:38,3,0:1:0,1:11
+chrIV	16951	.	C	A	76.0	.	DP=3;VDB=0.740702;SGB=-0.8159;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,2,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:35,3,0:1:0,1:11	1/1:65,6,0:2:0,2:13
+chrIV	17000	.	T	C	149.0	.	DP=7;VDB=0.103091;SGB=-0.680382;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,6,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:41,3,0:1:0,1:14	1/1:132,18,0:6:0,6:29
+chrIV	17020	.	C	G,A	99.0	.	DP=8;VDB=0.391417;SGB=2.55596;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0;AC=1,1;AN=4;DP4=0,1,7,0;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,3,34,3,34,34:1:1,0,0:3	1/2:150,33,15,125,0,122:7:0,6,1:10
+chrIV	17024	.	T	C	14.4249	.	DP=7;VDB=0.48;SGB=0.00473136;RPB=0.6;MQB=1;BQB=0.8;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=5,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:45,0,103:7:5,2:44
+chrIV	17037	.	T	A	37.8983	.	DP=7;VDB=0.460446;SGB=0.620439;RPB=1.01283;MQB=1.01283;BQB=0.810265;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,0,3,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:68,0,95:7:4,3:69
+chrIV	17051	.	C	T	103.0	.	DP=6;VDB=0.534935;SGB=1.41704;MQ0F=0;AC=2;AN=2;DP4=0,0,5,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:127,15,0:5:0,5:22
+chrIV	17112	.	T	A	3.03494	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:30,0,30:2:1,1:30
+chrV	5001	.	T	G	4.94666	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:28,3,0:1:0,1:4
+chrV	10045	.	C	T	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrV	10095	.	T	A	50.0	.	DP=2;VDB=0.56;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:75,6,0:2:0,2:9
+chrV	10108	.	C	A	4.15167	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:32,0,32:2:1,1:32
+chrV	10158	.	C	T	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrV	10166	.	G	A	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrV	10205	.	A	G	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrV	10229	.	G	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrV	10249	.	G	T	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrV	10250	.	C	T	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrV	10251	.	T	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrV	10257	.	A	T	6.39934	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:30,3,0:1:0,1:4
+chrV	10279	.	T	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrV	14271	.	T	C	39.4036	.	DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:64,6,0:2:0,2:9
+chrV	14273	.	G	C	39.4036	.	DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:64,6,0:2:0,2:9
+chrV	14285	.	T	C	76.0	.	DP=3;VDB=0.074936;SGB=0.461295;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,2,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:100,9,0:3:0,3:13
+chrV	14335	.	T	G	19.5632	.	DP=6;VDB=0.18;SGB=0.00473136;RPB=1;MQB=1;MQSB=1;BQB=0.875;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,0,0,2;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:50,0,98:6:4,2:49
+chrV	14337	.	A	G	100.0	.	DP=6;VDB=0.405004;SGB=1.41704;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,4,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:132,0,14:6:1,5:9
+chrV	14339	.	A	G	154.0	.	DP=6;VDB=0.189016;SGB=1.97062;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,4,2;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:178,18,0:6:0,6:25
+chrV	14346	.	C	T	100.0	.	DP=6;VDB=0.405004;SGB=1.41704;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,4,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:132,0,18:6:1,5:14
+chrV	14367	.	C	A	104.0	.	DP=5;VDB=0.220638;SGB=0.908071;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,2,2;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:128,12,0:4:0,4:19
+chrV	14445	.	A	G	21.4343	.	DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,0,2;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:46,6,0:2:0,2:9
+chrV	14481	.	G	A	4.15167	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:32,0,32:2:1,1:32
+chrV	14488	.	G	T	4.15167	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:32,0,32:2:1,1:32
+chrV	14528	.	G	A	3.56356	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:31,0,32:2:1,1:31
+chrV	14530	.	A	G	4.13076	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:32,0,22:2:1,1:23
+chrV	14538	.	T	C	32.4055	.	DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,0,2;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:57,6,0:2:0,2:9
+chrX	1592	.	T	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrX	1622	.	G	T	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrX	1648	.	A	C	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrX	1650	.	T	A	10.6576	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:35,3,0:1:0,1:4
+chrX	6015	.	A	T	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	1/1:38,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
+chrX	6032	.	G	A	8.01478	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	1/1:32,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
+chrX	6058	.	ta	t	15.3937	.	INDEL;IDV=1;IMF=1;DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:40,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
+chrX	6118	.	A	G	15.394	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:40,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
+chrX	6143	.	G	A	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:37,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
+chrX	6984	.	T	A	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrX	7025	.	A	G	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrX	10527	.	c	cC	8.86989	.	INDEL;IDV=1;IMF=1;DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:33,3,0:1:0,1:4
+chrX	10535	.	T	C	11.8912	.	DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:6
+chrX	10562	.	C	G	18.2649	.	DP=7;VDB=0.18;SGB=0.00473136;RPB=0.8;MQB=1;MQSB=1;BQB=0.9;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,1,2,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:49,0,131:7:5,2:48
+chrX	10595	.	T	G	148.0	.	DP=7;VDB=0.00429645;SGB=2.55596;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,6,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:172,21,0:7:0,7:29
+chrX	10629	.	gt	g	45.8065	.	INDEL;IDV=4;IMF=0.666667;DP=6;VDB=0.14;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:70,6,0:2:0,2:13
+chrX	10630	.	T	C,G	10.4616	.	DP=2;VDB=0.32;SGB=0.0985265;MQSB=1;MQ0F=0;AC=1,1;AN=2;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0,0,0,0:0:0,0,0:0	1/2:58,29,26,32,0,29:2:0,1,1:25
+chrX	10643	.	G	T	34.5919	.	DP=3;VDB=0.02;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:59,6,0:2:0,2:11
+chrX	15480	.	T	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrX	15495	.	G	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrX	24890	.	C	G	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrX	24935	.	A	C	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+MtDNA	954	.	G	T	4.79534	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=0,1,0,1;MQ=60	GT:PL:DP:DPR:GQ	0/1:36,3,0:1:0,1:3	0/1:0,3,33:1:1,0:3
+MtDNA	5979	.	C	A	7.18923	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:31,3,0:1:0,1:4
+MtDNA	8429	.	A	G	38.5774	.	DP=2;VDB=0.36;SGB=-0.759771;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:26,3,0:1:0,1:9	1/1:37,3,0:1:0,1:9
+MtDNA	12134	.	A	G	6.22224	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=0,1,0,1;MQ=60	GT:PL:DP:DPR:GQ	0/1:0,3,41:1:1,0:3	0/1:38,3,0:1:0,1:3
+MtDNA	12637	.	G	T	8.72328	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=1,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:41,3,0:1:0,1:3	0/1:0,3,25:1:1,0:3
+MtDNA	12998	.	C	T	14.4264	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	1/1:39,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
diff -r 000000000000 -r f0f2795de2c7 test-data/a_part1.bam
Binary file test-data/a_part1.bam has changed
diff -r 000000000000 -r f0f2795de2c7 test-data/a_part2.bam
Binary file test-data/a_part2.bam has changed
diff -r 000000000000 -r f0f2795de2c7 test-data/a_part2.bcf
Binary file test-data/a_part2.bcf has changed
diff -r 000000000000 -r f0f2795de2c7 test-data/ce11ToCe10.over.chain
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/ce11ToCe10.over.chain	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,871 @@
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diff -r 000000000000 -r f0f2795de2c7 test-data/header_only.bam
Binary file test-data/header_only.bam has changed
diff -r 000000000000 -r f0f2795de2c7 test-data/header_only.sam
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/header_only.sam	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,2 @@
+@HD	VN:1.5
+@RG	ID:000	SM:N2	DS:C. elegans wt	CN:ABC	DT:2017-06-27	LB:XYZ	PI:400	PL:ILLUMINA	PU:SEQ123
diff -r 000000000000 -r f0f2795de2c7 test-data/reads_1_w_header.bam
Binary file test-data/reads_1_w_header.bam has changed
diff -r 000000000000 -r f0f2795de2c7 test-data/reads_1and2_w_header.bam
Binary file test-data/reads_1and2_w_header.bam has changed
diff -r 000000000000 -r f0f2795de2c7 test-data/rebased.vcf
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/rebased.vcf	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,186 @@
+##fileformat=VCFv4.2
+##FILTER=<ID=PASS,Description="All filters passed">
+##reference=/home/wolma/galaxy_2017/database/files/000/dataset_35.dat
+##contig=<ID=chrI,length=15072423>
+##contig=<ID=chrII,length=15279345>
+##contig=<ID=chrIII,length=13783700>
+##contig=<ID=chrIV,length=17493793>
+##contig=<ID=chrM,length=13794>
+##contig=<ID=chrV,length=20924149>
+##contig=<ID=chrX,length=17718866>
+##ALT=<ID=X,Description="Represents allele(s) other than observed.">
+##INFO=<ID=INDEL,Number=0,Type=Flag,Description="Indicates that the variant is an INDEL.">
+##INFO=<ID=IDV,Number=1,Type=Integer,Description="Maximum number of reads supporting an indel">
+##INFO=<ID=IMF,Number=1,Type=Float,Description="Maximum fraction of reads supporting an indel">
+##INFO=<ID=DP,Number=1,Type=Integer,Description="Raw read depth">
+##INFO=<ID=VDB,Number=1,Type=Float,Description="Variant Distance Bias for filtering splice-site artefacts in RNA-seq data (bigger is better)",Version="3">
+##INFO=<ID=RPB,Number=1,Type=Float,Description="Mann-Whitney U test of Read Position Bias (bigger is better)">
+##INFO=<ID=MQB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality Bias (bigger is better)">
+##INFO=<ID=BQB,Number=1,Type=Float,Description="Mann-Whitney U test of Base Quality Bias (bigger is better)">
+##INFO=<ID=MQSB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality vs Strand Bias (bigger is better)">
+##INFO=<ID=SGB,Number=1,Type=Float,Description="Segregation based metric.">
+##INFO=<ID=MQ0F,Number=1,Type=Float,Description="Fraction of MQ0 reads (smaller is better)">
+##INFO=<ID=ICB,Number=1,Type=Float,Description="Inbreeding Coefficient Binomial test (bigger is better)">
+##INFO=<ID=HOB,Number=1,Type=Float,Description="Bias in the number of HOMs number (smaller is better)">
+##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes for each ALT allele, in the same order as listed">
+##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
+##INFO=<ID=DP4,Number=4,Type=Integer,Description="Number of high-quality ref-forward , ref-reverse, alt-forward and alt-reverse bases">
+##INFO=<ID=MQ,Number=1,Type=Integer,Description="Average mapping quality">
+##FORMAT=<ID=PL,Number=G,Type=Integer,Description="List of Phred-scaled genotype likelihoods">
+##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Number of high-quality bases">
+##FORMAT=<ID=DPR,Number=R,Type=Integer,Description="Number of high-quality bases observed for each allele">
+##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
+##FORMAT=<ID=GQ,Number=1,Type=Integer,Description="Phred-scaled Genotype Quality">
+##source=MiModD varcall (version 0.1.8.0)
+##MiModDCommand=mimodd varcall /home/wolma/galaxy_2017/database/files/000/dataset_35.dat /home/wolma/galaxy_2017/database/files/000/dataset_54.dat --index-files /home/wolma/galaxy_2017/database/files/_metadata_files/000/metadata_14.dat --ofile /home/wolma/galaxy_2017/database/files/000/dataset_58.dat --depth 250 --verbose --quiet
+##samtoolsCommand=n.a. (wrapped by MiModD)
+##bcftools_callCommand=n.a. (wrapped by MiModD)
+##rginfo=<ID=0,Rgid="000",Name="N2">
+##rginfo=<ID=1,Rgid="266-1",Name="ot266",Description="mutant strain carrying the vab-3 allele ot266">
+##samtoolsVersion=1.2+htslib-1.2.1
+##bcftools_callVersion=1.2+htslib-1.2.1
+##bcftools_concatVersion=1.2+htslib-1.2.1
+##bcftools_viewVersion=1.2+htslib-1.2.1
+##bcftools_viewCommand=view /home/wolma/galaxy_2017/database/files/000/dataset_58.dat
+#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	N2	ot266
+chrI	855	.	G	C	231.0	.	DP=27;VDB=2.48853e-13;SGB=13.8822;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,11,13;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:255,72,0:24:0,24:86
+chrI	885	.	t	tTCT	69.0	.	INDEL;IDV=4;IMF=0.0769231;DP=56;VDB=2.27029e-09;SGB=1.27626;MQSB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=16,17,3,1;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,3,60:1:1,0:11	0/1:105,0,255:36:32,4:96
+chrI	912	.	A	T	195.0	.	DP=92;VDB=0.233506;SGB=-8.71232;RPB=0.810719;MQB=1;MQSB=1;BQB=0.974933;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=30,38,9,13;MQ=60	GT:PL:DP:DPR:GQ	0/1:26,0,54:2:1,1:25	0/1:204,0,255:88:67,21:127
+chrI	921	.	G	A	341.0	.	DP=101;VDB=0.0381167;SGB=14.497;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;AC=4;AN=4;DP4=0,1,41,52;MQ=60	GT:PL:DP:DPR:GQ	1/1:110,6,0:2:0,2:25	1/1:255,239,0:92:1,91:127
+chrI	942	.	A	T	182.0	.	DP=147;VDB=0.486211;SGB=-13.0084;RPB=0.177913;MQB=1;MQSB=1;BQB=0.00895523;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=42,63,15,21;MQ=60	GT:PL:DP:DPR:GQ	0/1:20,0,116:4:3,1:17	0/1:198,0,255:137:102,35:127
+chrI	945	.	G	A	396.0	.	DP=144;VDB=0.936097;SGB=17.553;RPB=0.198891;MQB=1;MQSB=1;BQB=0.921548;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=7,15,50,66;MQ=60	GT:PL:DP:DPR:GQ	1/1:177,12,0:4:0,4:19	0/1:255,0,86:134:22,112:78
+chrI	962	.	G	T	379.0	.	DP=141;VDB=0.0554366;SGB=20.8365;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,58,75;MQ=60	GT:PL:DP:DPR:GQ	1/1:148,12,0:4:0,4:36	1/1:255,255,0:129:0,129:127
+chrI	973	.	A	G,T	329.0	.	DP=140;VDB=0.0208629;SGB=18.7462;RPB=0.14071;MQB=1;MQSB=1;BQB=0.978744;MQ0F=0;ICB=0.3;HOB=0.125;AC=3,0;AN=4;DP4=7,12,44,71;MQ=60	GT:PL:DP:DPR:GQ	1/1:110,6,0,110,6,110:2:0,2,0:14	0/1:255,0,65,255,255,255:132:19,112,1:56
+chrI	974	.	A	T	9.21175	.	DP=139;VDB=0.000145084;SGB=10.9211;RPB=0.0841531;MQB=1;MQSB=1;BQB=0.939818;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=44,72,7,11;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,6,110:2:2,0:14	0/1:45,0,255:132:114,18:36
+chrI	998	.	A	T	99.0	.	DP=98;VDB=0.00196934;SGB=10.9211;RPB=0.976264;MQB=1;MQSB=1;BQB=0.993458;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=33,40,5,13;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,6,110:2:2,0:12	0/1:134,0,255:89:71,18:127
+chrI	1000	.	A	T	93.0	.	DP=108;VDB=0.000325892;SGB=11.6141;RPB=0.945574;MQB=1;MQSB=1;BQB=0.923217;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=34,48,9,10;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,6,110:2:2,0:13	0/1:128,0,255:99:80,19:120
+chrI	1028	.	G	A	105.0	.	DP=63;VDB=0.000108954;SGB=6.07412;RPB=0.732968;MQB=1;MQSB=1;BQB=0.91987;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=18,30,6,5;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,6,110:2:2,0:12	0/1:140,0,255:57:46,11:127
+chrI	1041	.	C	T	6.52941	.	DP=40;VDB=0.00951951;SGB=1.94933;RPB=0.416648;MQB=1;MQSB=1;BQB=0.652425;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=13,21,3,2;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,6,71:2:2,0:14	0/1:42,0,255:37:32,5:33
+chrI	1045	.	T	A	273.0	.	DP=36;VDB=1.8168e-09;SGB=2.16465;RPB=0.578971;MQB=1;MQSB=1;BQB=0.637911;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=4,1,11,18;MQ=60	GT:PL:DP:DPR:GQ	1/1:54,6,0:2:0,2:13	0/1:255,0,51:32:5,27:43
+chrI	1048	.	G	A	266.0	.	DP=30;VDB=1.83438e-10;SGB=2.65329;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,10,18;MQ=60	GT:PL:DP:DPR:GQ	1/1:35,3,0:1:0,1:20	1/1:255,81,0:27:0,27:98
+chrI	1053	.	c	cT	8.64418	.	INDEL;IDV=1;IMF=0.047619;DP=21;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:32,3,0:1:0,1:16
+chrI	1054	.	A	T	231.0	.	DP=18;VDB=4.22339e-07;SGB=7.05893;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,5,9;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:255,42,0:14:0,14:54
+chrI	1412	.	T	C	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrI	1414	.	G	A	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrI	1421	.	G	A	10.6576	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:35,3,0:1:0,1:4
+chrI	2752	.	C	T	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrI	13851	.	T	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrI	13856	.	T	A	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrI	13874	.	A	G	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrI	13876	.	C	T	10.6576	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:35,3,0:1:0,1:4
+chrI	13901	.	C	A	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrI	13919	.	C	A	9.75336	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:34,3,0:1:0,1:4
+chrI	13921	.	T	C	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	13944	.	T	A	11.8912	.	DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:6
+chrI	13952	.	G	T	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrI	13958	.	C	T	8.01478	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:32,3,0:1:0,1:4
+chrI	13970	.	C	T	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	13993	.	A	G	10.6576	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:35,3,0:1:0,1:4
+chrI	14201	.	G	A	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	14223	.	A	G	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	14242	.	T	C	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	14246	.	C	T	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	14247	.	T	C	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	22325	.	T	C	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrI	23943	.	g	gG	41.4034	.	INDEL;IDV=2;IMF=1;DP=2;VDB=0.94;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:66,6,0:2:0,2:9
+chrII	239	.	A	G	9.75336	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:34,3,0:1:0,1:4
+chrII	252	.	A	T	44.4523	.	DP=11;VDB=0.0411132;SGB=0.620439;RPB=0.916482;MQB=0.916482;MQSB=1.00775;BQB=0.366593;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,3,2,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:75,0,160:10:7,3:74
+chrII	265	.	A	G	161.0	.	DP=14;VDB=0.00228484;SGB=4.69269;RPB=0.965069;MQB=1;MQSB=0.966012;BQB=0.686908;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,1,3,6;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:191,0,96:14:5,9:96
+chrII	278	.	A	T	167.0	.	DP=14;VDB=0.000574122;SGB=4.69269;RPB=0.249352;MQB=1;MQSB=0.966012;BQB=0.867428;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=2,3,5,4;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:197,0,111:14:5,9:111
+chrII	279	.	A	T,G	38.2143	.	DP=14;VDB=0.0501588;SGB=1.94933;RPB=0.448332;MQB=1;MQSB=0.966012;BQB=0.118179;MQ0F=0;ICB=1;HOB=0.5;AC=1,0;AN=2;DP4=7,2,0,5;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0,0,0,0:0:0,0,0:0	0/1:69,0,186,71,175,247:14:9,4,1:67
+chrII	291	.	A	G	86.0	.	DP=14;VDB=0.0340507;SGB=1.94933;RPB=1;MQB=1;MQSB=0.966012;BQB=0.197899;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=6,3,1,4;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:116,0,189:14:9,5:116
+chrII	300	.	A	C	231.0	.	DP=14;VDB=3.24567e-05;SGB=7.05893;MQSB=0.966012;MQ0F=0;AC=2;AN=2;DP4=0,0,7,7;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:255,42,0:14:0,14:53
+chrII	304	.	A	T	137.0	.	DP=14;VDB=0.000156924;SGB=4.00342;RPB=0.991701;MQB=1;MQSB=0.966012;BQB=0.703221;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,5,6,2;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:167,0,107:14:6,8:108
+chrII	305	.	A	T	27.2811	.	DP=14;VDB=0.0785113;SGB=0.620439;RPB=0.973096;MQB=1;MQSB=0.966012;BQB=0.738577;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=6,5,1,2;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:59,0,236:14:11,3:56
+chrII	317	.	A	G	101.0	.	DP=14;VDB=0.00324086;SGB=2.63044;RPB=0.94925;MQB=1;MQSB=0.966012;BQB=0.997919;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,4,3,3;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:131,0,172:14:8,6:127
+chrII	331	.	A	T	121.0	.	DP=14;VDB=0.0126037;SGB=3.31571;RPB=0.497296;MQB=0.966012;MQSB=0.966012;BQB=0.834543;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=5,2,2,5;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:151,0,154:14:7,7:127
+chrII	343	.	A	G	90.0	.	DP=9;VDB=0.00476095;SGB=1.97062;RPB=0.346719;MQB=0.924584;MQSB=0.924584;BQB=0.462292;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,2,5,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:120,0,74:9:3,6:74
+chrII	11921	.	A	T	7.18923	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:31,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
+chrII	17381	.	G	A	4.14838	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:32,0,34:2:1,1:32	./.:0,0,0:0:0,0:0
+chrII	17518	.	A	T	14.9987	.	DP=3;VDB=0.5;SGB=0.0985265;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,2,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:45,0,29:3:1,2:30	./.:0,0,0:0:0,0:0
+chrII	19959	.	T	A	7.18923	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:31,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
+chrIII	7994	.	C	T	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrIII	8004	.	T	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrIII	8023	.	A	G	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrIII	8026	.	A	G	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrIII	8027	.	A	G	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrIII	8039	.	C	A	9.75336	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:34,3,0:1:0,1:4
+chrIII	9659	.	C	T	9.75336	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:34,3,0:1:0,1:4
+chrIII	9662	.	G	T	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrIII	9700	.	A	T	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrIV	9450	.	A	G	4.78389	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:33,0,27:2:1,1:28	./.:0,0,0:0:0,0:0
+chrIV	16467	.	T	C	4.14182	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:32,0,25:2:1,1:26	./.:0,0,0:0:0,0:0
+chrIV	16478	.	C	G	4.58205	.	DP=3;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=0,2,1,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:35,0,23:2:1,1:28	0/0:0,3,29:1:1,0:5
+chrIV	16499	.	A	G	3.42278	.	DP=3;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=1,1,0,1;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,6,79:2:2,0:4	0/1:35,3,0:1:0,1:3
+chrIV	16537	.	aaga	a	25.8002	.	INDEL;IDV=1;IMF=1;DP=3;VDB=0.206307;SGB=-0.516033;MQSB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=1;AN=4;DP4=1,1,0,1;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,6,120:2:2,0:4	0/1:60,3,0:1:0,1:3
+chrIV	16633	.	C	A	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrIV	16697	.	C	T	52.0	.	DP=2;VDB=0.48;SGB=-0.759771;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:40,3,0:1:0,1:9	1/1:36,3,0:1:0,1:9
+chrIV	16709	.	C	G	5.30774	.	DP=3;VDB=0.98;SGB=0.0985265;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.3;HOB=0.125;AC=3;AN=4;DP4=0,1,2,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:0,3,39:1:1,0:3	1/1:38,6,0:2:0,2:4
+chrIV	16729	.	C	T	4.14943	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=0.5;HOB=0.5;AC=2;AN=4;DP4=0,1,1,0;MQ=60	GT:PL:DP:DPR:GQ	0/1:0,3,37:1:1,0:3	0/1:35,3,0:1:0,1:3
+chrIV	16769	.	A	T	13.7861	.	DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:6
+chrIV	16814	.	A	G	3.03341	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:30,0,31:2:1,1:30
+chrIV	16815	.	C	A	48.4033	.	DP=2;VDB=0.42;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:73,6,0:2:0,2:9
+chrIV	16823	.	A	C	4.14995	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:32,0,28:2:1,1:29
+chrIV	16829	.	T	C	3.56356	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:31,0,32:2:1,1:31
+chrIV	16833	.	A	T,G	14.5409	.	DP=2;VDB=0.42;SGB=0.0985265;MQSB=1;MQ0F=0;AC=1,1;AN=2;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0,0,0,0:0:0,0,0:0	1/2:65,34,31,34,0,31:2:0,1,1:29
+chrIV	16834	.	T	A	3.56452	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=1,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:31,0,27:2:1,1:28
+chrIV	16894	.	T	C	55.0	.	DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	1/1:41,3,0:1:0,1:9	1/1:38,3,0:1:0,1:9
+chrIV	16899	.	T	A	49.5769	.	DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	1/1:41,3,0:1:0,1:9	1/1:33,3,0:1:0,1:9
+chrIV	16902	.	T	G	55.0	.	DP=2;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	1/1:41,3,0:1:0,1:9	1/1:38,3,0:1:0,1:9
+chrIV	16921	.	A	G	68.0	.	DP=3;VDB=0.58;SGB=-0.759771;MQ0F=0;AC=4;AN=4;DP4=0,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	1/1:54,3,0:1:0,1:11	1/1:38,3,0:1:0,1:11
+chrIV	16951	.	C	A	76.0	.	DP=3;VDB=0.740702;SGB=-0.8159;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,2,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:35,3,0:1:0,1:11	1/1:65,6,0:2:0,2:13
+chrIV	17000	.	T	C	149.0	.	DP=7;VDB=0.103091;SGB=-0.680382;MQSB=1;MQ0F=0;AC=4;AN=4;DP4=0,0,6,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:41,3,0:1:0,1:14	1/1:132,18,0:6:0,6:29
+chrIV	17020	.	C	G,A	99.0	.	DP=8;VDB=0.391417;SGB=2.55596;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0;AC=1,1;AN=4;DP4=0,1,7,0;MQ=60	GT:PL:DP:DPR:GQ	0/0:0,3,34,3,34,34:1:1,0,0:3	1/2:150,33,15,125,0,122:7:0,6,1:10
+chrIV	17024	.	T	C	14.4249	.	DP=7;VDB=0.48;SGB=0.00473136;RPB=0.6;MQB=1;BQB=0.8;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=5,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:45,0,103:7:5,2:44
+chrIV	17037	.	T	A	37.8983	.	DP=7;VDB=0.460446;SGB=0.620439;RPB=1.01283;MQB=1.01283;BQB=0.810265;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,0,3,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:68,0,95:7:4,3:69
+chrIV	17051	.	C	T	103.0	.	DP=6;VDB=0.534935;SGB=1.41704;MQ0F=0;AC=2;AN=2;DP4=0,0,5,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:127,15,0:5:0,5:22
+chrIV	17112	.	T	A	3.03494	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:30,0,30:2:1,1:30
+chrV	5001	.	T	G	4.94666	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:28,3,0:1:0,1:4
+chrV	10045	.	C	T	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrV	10095	.	T	A	50.0	.	DP=2;VDB=0.56;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:75,6,0:2:0,2:9
+chrV	10108	.	C	A	4.15167	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:32,0,32:2:1,1:32
+chrV	10158	.	C	T	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrV	10166	.	G	A	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrV	10205	.	A	G	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrV	10229	.	G	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrV	10249	.	G	T	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrV	10250	.	C	T	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
+chrV	10251	.	T	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrV	10257	.	A	T	6.39934	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:30,3,0:1:0,1:4
+chrV	10279	.	T	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrV	14271	.	T	C	39.4036	.	DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:64,6,0:2:0,2:9
+chrV	14273	.	G	C	39.4036	.	DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:64,6,0:2:0,2:9
+chrV	14285	.	T	C	76.0	.	DP=3;VDB=0.074936;SGB=0.461295;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,2,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:100,9,0:3:0,3:13
+chrV	14335	.	T	G	19.5632	.	DP=6;VDB=0.18;SGB=0.00473136;RPB=1;MQB=1;MQSB=1;BQB=0.875;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,0,0,2;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:50,0,98:6:4,2:49
+chrV	14337	.	A	G	100.0	.	DP=6;VDB=0.405004;SGB=1.41704;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,4,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:132,0,14:6:1,5:9
+chrV	14339	.	A	G	154.0	.	DP=6;VDB=0.189016;SGB=1.97062;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,4,2;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:178,18,0:6:0,6:25
+chrV	14346	.	C	T	100.0	.	DP=6;VDB=0.405004;SGB=1.41704;RPB=1;MQB=1;MQSB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,4,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:132,0,18:6:1,5:14
+chrV	14367	.	C	A	104.0	.	DP=5;VDB=0.220638;SGB=0.908071;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,2,2;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:128,12,0:4:0,4:19
+chrV	14445	.	A	G	21.4343	.	DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,0,2;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:46,6,0:2:0,2:9
+chrV	14481	.	G	A	4.15167	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:32,0,32:2:1,1:32
+chrV	14488	.	G	T	4.15167	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:32,0,32:2:1,1:32
+chrV	14528	.	G	A	3.56356	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:31,0,32:2:1,1:31
+chrV	14530	.	A	G	4.13076	.	DP=2;SGB=-0.516033;RPB=1;MQB=1;BQB=1;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=0,1,0,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:32,0,22:2:1,1:23
+chrV	14538	.	T	C	32.4055	.	DP=2;VDB=0.06;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,0,2;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:57,6,0:2:0,2:9
+chrX	1592	.	T	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrX	1622	.	G	T	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrX	1648	.	A	C	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrX	1650	.	T	A	10.6576	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:35,3,0:1:0,1:4
+chrX	6015	.	A	T	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	1/1:38,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
+chrX	6032	.	G	A	8.01478	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	1/1:32,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
+chrX	6058	.	ta	t	15.3937	.	INDEL;IDV=1;IMF=1;DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:40,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
+chrX	6118	.	A	G	15.394	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:40,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
+chrX	6143	.	G	A	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,0,1;MQ=60	GT:PL:DP:DPR:GQ	1/1:37,3,0:1:0,1:4	./.:0,0,0:0:0,0:0
+chrX	6984	.	T	A	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrX	7025	.	A	G	12.5173	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:37,3,0:1:0,1:4
+chrX	10527	.	c	cC	8.86989	.	INDEL;IDV=1;IMF=1;DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:33,3,0:1:0,1:4
+chrX	10535	.	T	C	11.8912	.	DP=2;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:6
+chrX	10562	.	C	G	18.2649	.	DP=7;VDB=0.18;SGB=0.00473136;RPB=0.8;MQB=1;MQSB=1;BQB=0.9;MQ0F=0;ICB=1;HOB=0.5;AC=1;AN=2;DP4=4,1,2,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	0/1:49,0,131:7:5,2:48
+chrX	10595	.	T	G	148.0	.	DP=7;VDB=0.00429645;SGB=2.55596;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,6,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:172,21,0:7:0,7:29
+chrX	10629	.	gt	g	45.8065	.	INDEL;IDV=4;IMF=0.666667;DP=6;VDB=0.14;SGB=0.0985265;MQ0F=0;AC=2;AN=2;DP4=0,0,2,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:70,6,0:2:0,2:13
+chrX	10630	.	T	C,G	10.4616	.	DP=2;VDB=0.32;SGB=0.0985265;MQSB=1;MQ0F=0;AC=1,1;AN=2;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0,0,0,0:0:0,0,0:0	1/2:58,29,26,32,0,29:2:0,1,1:25
+chrX	10643	.	G	T	34.5919	.	DP=3;VDB=0.02;SGB=0.0985265;MQSB=1;MQ0F=0;AC=2;AN=2;DP4=0,0,1,1;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:59,6,0:2:0,2:11
+chrX	15480	.	T	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrX	15495	.	G	C	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrX	24890	.	C	G	13.4669	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:38,3,0:1:0,1:4
+chrX	24935	.	A	C	11.5799	.	DP=1;SGB=-0.157211;MQ0F=0;AC=2;AN=2;DP4=0,0,1,0;MQ=60	GT:PL:DP:DPR:GQ	./.:0,0,0:0:0,0:0	1/1:36,3,0:1:0,1:4
diff -r 000000000000 -r f0f2795de2c7 test-data/so_coordinate.bam
Binary file test-data/so_coordinate.bam has changed
diff -r 000000000000 -r f0f2795de2c7 test-data/so_queryname.sam
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/so_queryname.sam	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,2 @@
+@HD	VN:1.5	SO:queryname
+@RG	ID:000	SM:N2	DS:C. elegans wt	CN:ABC	DT:2017-06-27	LB:XYZ	PI:400	PL:ILLUMINA	PU:SEQ123
diff -r 000000000000 -r f0f2795de2c7 test-data/split_pair_reads_1.fastqsanger
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/split_pair_reads_1.fastqsanger	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,20 @@
+@HWI-EAS91_1_30788AAXX:7:21:1542:1758/1
+GTCAATTGTACTGGTCAATACTAAAAGAATAGGATC
++HWI-EAS91_1_30788AAXX:7:21:1542:1758/1
+hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh
+@HWI-EAS91_1_30788AAXX:7:22:1621:462/1
+ATAATGGCTATTATTGTGGGGGGGATGATGCTGGAA
++HWI-EAS91_1_30788AAXX:7:22:1621:462/1
+hhhhhhhhhhhhQAhh@hhhhNhhhfhMbCIScC?h
+@HWI-EAS91_1_30788AAXX:7:45:408:807/1
+TACCCGATTTTTTGCTTTCCACTTTATCCTACCCTT
++HWI-EAS91_1_30788AAXX:7:45:408:807/1
+hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh
+@HWI-EAS91_1_30788AAXX:7:49:654:1439/1
+CTAACTCTATTTATTGTATTTCAACTAAAAATCTCA
++HWI-EAS91_1_30788AAXX:7:49:654:1439/1
+hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh
+@HWI-EAS91_1_30788AAXX:7:64:947:234/1
+TATCAAAAAAGAATATAATCTGAATCAACACTACAA
++HWI-EAS91_1_30788AAXX:7:64:947:234/1
+hhhhhhhhhhhhhhhhhhhhhhhRhhehhahhhhhJ
diff -r 000000000000 -r f0f2795de2c7 test-data/split_pair_reads_2.fastqsanger
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/split_pair_reads_2.fastqsanger	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,20 @@
+@HWI-EAS91_1_30788AAXX:7:21:1542:1758/2
+GCTCCTAGCATCTGGAGTCTCTATCACCTGAGCCCA
++HWI-EAS91_1_30788AAXX:7:21:1542:1758/2
+hhhhhhhhhhhhhhhhhhhhhhhh`hfhhVZSWehR
+@HWI-EAS91_1_30788AAXX:7:22:1621:462/2
+ACTAGCCCCAATATCAATCCTATATCAAATCTCACC
++HWI-EAS91_1_30788AAXX:7:22:1621:462/2
+hJhhhhChhhJhhhRhhKhePhc\KhhV\KhXhJhh
+@HWI-EAS91_1_30788AAXX:7:45:408:807/2
+ATGAGTGCTAGGATCAGGATGGAGAGGATTAGGGCT
++HWI-EAS91_1_30788AAXX:7:45:408:807/2
+hhhhhhhhhhhhhhhhhh`hhhZh`hhhhhRXhhYh
+@HWI-EAS91_1_30788AAXX:7:49:654:1439/2
+TAGGTTTATTGATAGTTGTGTTGTTGGTGTAAATGG
++HWI-EAS91_1_30788AAXX:7:49:654:1439/2
+hhhhhhhhhhhhhhhhhhhhhhhhhdhh_hG\XhU@
+@HWI-EAS91_1_30788AAXX:7:64:947:234/2
+CCTATTAGTGTGTAGAATAGGAAGTAGAGGCCTGCG
++HWI-EAS91_1_30788AAXX:7:64:947:234/2
+hhhhhhhh^hPhWfhhhhThWUhhfhh_hhNIVPUd
diff -r 000000000000 -r f0f2795de2c7 test-data/vaf_linkage.pdf
Binary file test-data/vaf_linkage.pdf has changed
diff -r 000000000000 -r f0f2795de2c7 tool-data/all_fasta.loc.sample
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool-data/all_fasta.loc.sample	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,18 @@
+#This file lists the locations and dbkeys of all the fasta files
+#under the "genome" directory (a directory that contains a directory
+#for each build). The script extract_fasta.py will generate the file
+#all_fasta.loc. This file has the format (white space characters are
+#TAB characters):
+#
+#<unique_build_id> <dbkey> <display_name> <file_path>
+#
+#So, all_fasta.loc could look something like this:
+#
+#apiMel3 apiMel3 Honeybee (Apis mellifera): apiMel3 /path/to/genome/apiMel3/apiMel3.fa
+#hg19canon hg19 Human (Homo sapiens): hg19 Canonical /path/to/genome/hg19/hg19canon.fa
+#hg19full hg19 Human (Homo sapiens): hg19 Full /path/to/genome/hg19/hg19full.fa
+#
+#Your all_fasta.loc file should contain an entry for each individual
+#fasta file. So there will be multiple fasta files for each build,
+#such as with hg19 above.
+#
diff -r 000000000000 -r f0f2795de2c7 tool_data_table_conf.xml.sample
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_data_table_conf.xml.sample	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,7 @@
+<tables>
+    <!-- Locations of all fasta files under genome directory -->
+    <table name="all_fasta" comment_char="#">
+        <columns>value, dbkey, name, path</columns>
+        <file path="tool-data/all_fasta.loc" />
+    </table>
+</tables>
diff -r 000000000000 -r f0f2795de2c7 varextract.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/varextract.xml	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,184 @@
+<tool id="mimodd_varextract" name="MiModD Extract Variant Sites" 
+version="@MIMODD_WRAPPER_VERSION@">
+    <description>from a BCF file</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command><![CDATA[
+	mimodd varextract '$ifile'
+	  #if $len($sitesinfo)
+	    -p
+	    #for $source in $sitesinfo
+          '${source.pre_vcf}'
+	    #end for
+	  #end if
+	  --ofile '$output_vcf'
+	  $keep_alts
+	  --verbose
+    ]]></command>
+
+    <inputs>
+        <param name="ifile" type="data" format="bcf" label="BCF input file"
+        help="Use the MiModD Variant Calling tool to generate the input for this tool."/>
+        <repeat name="sitesinfo" title="include information from pre-calculated vcf dataset" default="0">
+	        <param name="pre_vcf" type="data" format="vcf"
+	        label="independently generated vcf datset" />
+        </repeat>
+        <param name="keep_alts" type="boolean" truevalue="-a" falsevalue="" checked="false"
+        label="keep all sites with alternate bases"
+        help="If selected, the VCF output will include ALL sites for which non-reference bases have been observed, i.e., even those not considered allelic sites by the variant caller." />
+    </inputs>
+    <outputs>
+        <data name="output_vcf" format="vcf"
+        label="Variants extracted with MiModd from ${on_string}"/>
+    </outputs>
+
+    <tests>
+        <test>
+            <param name="ifile" value="a.bcf" />
+            <output name="output_vcf" ftype="vcf">
+                <assert_contents>
+                    <has_line_matching expression="#CHROM.POS.ID.REF.ALT.QUAL.FILTER.INFO.FORMAT.N2.ot266" />
+                </assert_contents>
+            </output>
+            <assert_command>
+                <not_has_text text="-a" />
+            </assert_command>
+        </test>
+        <test>
+            <param name="ifile" value="a_part2.bcf" />
+            <param name="keep_alts" value="true" />
+            <param name="pre_vcf" value="a.vcf" />
+            <output name="output_vcf" ftype="vcf">
+                <assert_contents>
+                    <has_line_matching expression="#CHROM.POS.ID.REF.ALT.QUAL.FILTER.INFO.FORMAT.ot266.external_source_1_N2.external_source_1_ot266" />
+                </assert_contents>
+            </output>
+            <assert_command>
+                <has_text text="-a" />
+            </assert_command>
+        </test>
+    </tests>
+    <help><![CDATA[
+.. class:: infomark
+
+   **What it does**
+
+The tool takes as input a BCF dataset like the ones produced by the
+*MiModD Variant Calling* tool, extracts just the variant sites from it and
+reports them in VCF format.
+
+If the BCF input file specifies multiple samples, sites are included if they qualify as variant sites in at least one sample.
+
+----------
+
+**Options:**
+
+**keep all sites with alternate bases**
+
+By default, a variant site is considered to be a position in the genome for
+which a non-reference allele appears in the inferred genotype of any sample. 
+
+You can select the *keep all sites with alternate bases* option, if instead
+you want to extract all sites, for which at least one non-reference base has
+been observed (whether resulting in a non-reference allele call or not).
+Using this option should rarely be necessary, but could be occassionally
+helpful for closer inspection of candidate genomic regions.
+
+ 
+**include information from pre-calculated vcf dataset**
+
+During the process of variant extraction the tool can take into account
+genome positions specified in one or more independently generated VCF datasets.
+If such additional VCF input is provided, the tool output will contain the
+samples found in these files as additional samples and sites from the main BCF
+dataset will be included not only if they qualify as variant sites in at least
+one sample specified in the BCF, but also if they are listed in any of the
+additional VCF datasets.
+
+Optional VCF input can be particularly useful in one of the following
+situations:
+   
+1) you have prior information that leads you to think that certain genome
+   positions are of special relevance for your project and, thus, you are
+   interested in the statistics produced by the variant caller for these
+   positions even if they are not considered variant sites. In this case you
+   can use a minimal VCF dataset to guide the variant extraction process to
+   include these positions. This dataset needs a minimal header of the form:
+
+   ``##fileformat=VCFv4.2``
+
+   followed by positional information like in this example::
+ 						     
+     #CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO
+     chrI	1222	.	.	.	.	.	.
+     chrI	2651	.	.	.	.	.	.
+     chrI	3659	.	.	.	.	.	.
+     chrI	3731	.	.	.	.	.	.
+  
+   , where columns are tab-separated and . serves as a placeholder for missing
+   information.
+  
+2) you have actual variant calls from an additional sample, but you do not
+   have access to the original sequenced reads data (if you had, the
+   recommended approach would be to include that data in the
+   *MiModD Variant Calling* step. 
+
+   This situation is often encountered with published datasets. Assume you
+   have obtained a list of known single nucleotide variants (SNVs) found in
+   one particular strain of your favorite model organism and you would like
+   to know which of these SNVs are present in the related strains you have
+   sequenced. You have aligned the sequenced reads from your samples and have
+   used the *MiModD Variant Calling* tool, which has generated a BCF dataset
+   ready for variant extraction. If the SNV list for the previously sequenced
+   strain is in VCF format already, you can now just plug it into the
+   analysis process by specifying it in the tool interface as an
+   *independently generated vcf dataset*.
+   The resulting vcf output will contain all SNV sites along with the variant
+   sites found in the BCF alone. You can then proceed to the
+   *MiModD VCF Filter* tool to look at the original SNV sites only or to
+   investigate any other interesting subset of sites. If the SNV list is in
+   some other format, you will have o convert it to VCF first. At a minimum,
+   the dataset must have a ``##fileformat`` header line like the previous
+   example and have the ``REF`` and ``ALT`` column filled in like so::
+
+     #CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO
+     chrI	1897409	.	A	G	.	.	.
+     chrI	1897492	.	C	T	.	.	.
+     chrI	1897616	.	C	A	.	.	.
+     chrI	1897987	.	A	T	.	.	.
+     chrI	1898185	.	C	T	.	.	.
+     chrI	1898715	.	G	A	.	.	.
+     chrI	1898729	.	T	C	.	.	.
+     chrI	1900288	.	T	A	.	.	.
+  
+   , in which case the tool will assume that the corresponding sample is
+   homozygous for each of the SNVs.
+   If you need to distinguish between homozygous and heterozygous SNVs you
+   will have to extend the format to include a format and a sample column
+   with genotype (GT) information like in this example::
+
+     #CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	sampleX
+     chrI	1897409	.	A	G	.	.	.	GT	1/1
+     chrI	1897492	.	C	T	.	.	.	GT	0/1
+     chrI	1897616	.	C	A	.	.	.	GT	0/1
+     chrI	1897987	.	A	T	.	.	.	GT	0/1
+     chrI	1898185	.	C	T	.	.	.	GT	0/1
+     chrI	1898715	.	G	A	.	.	.	GT	0/1
+     chrI	1898729	.	T	C	.	.	.	GT	0/1
+     chrI	1900288	.	T	A	.	.	.	GT	0/1
+  
+   , in which sampleX would be heterozygous for all SNVs except the first.
+
+.. class:: warningmark
+   
+   If the optional VCF input contains INDEL calls, these will be ignored by the
+   tool.
+
+@HELP_FOOTER@
+    ]]></help>
+    <expand macro="citations" />
+</tool>
diff -r 000000000000 -r f0f2795de2c7 varreport.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/varreport.xml	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,142 @@
+<tool id="mimodd_varreport" name="MiModD Report Variants" 
+version="@MIMODD_WRAPPER_VERSION@">
+    <description>
+    in a human-friendly format that simplifies data exploration
+    </description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command><![CDATA[
+    #if $str($formatting.oformat) == 'html':
+      mkdir '$ofile.files_path' &&
+    #end if
+  	mimodd varreport
+  	  '$inputfile' --oformat ${formatting.oformat}
+	  #if $str($formatting.oformat) == 'html':
+	    #if $str($formatting.species):
+	      --species '${formatting.species}'
+	    #end if
+	    #if $formatting.link_formatter:
+	      --link '${formatting.link_formatter}'
+	    #end if
+	    --ofile '${ofile.files_path}/variant_report.html'
+	    && echo
+	    '<html><body><p>MiModD has generated the variant report you requested, but for technical reasons, we have to take you through this little detour to display the report in Galaxy.</p><p>Proceed to <a href="variant_report.html">the variant report</a>.</p></body></html>'
+	    > '$ofile'
+	  #else
+        --ofile '$ofile'
+	  #end if
+    ]]></command>
+
+    <inputs>
+        <param name="inputfile" type="data" format="vcf"
+        label="The VCF input with the variants to be reported" />
+        <conditional name="formatting">
+            <param name="oformat" type="select"
+            label="Format to use for the report">
+                <option value="html">HTML</option>
+                <option value="text">Tab-separated plain text</option>
+            </param>
+            <when value="html">
+                <param name="species" type="text" label="Species"
+                help="If you declare the species your input data comes from, variant reports in html format can have hyperlinks to species-specific databases and genome browsers added. If you have used the MiModD Variant Annotation tool to generate the input dataset, the species information will already be recorded in the dataset, but you can overwrite it if you wish.">
+                    <expand macro="lex_sam_header" message="Non-ASCII characters are not allowed in species names." />
+                </param>
+                <param name="link_formatter" type="data" format="txt" optional="true"
+                label="Optional file with species-specific hyperlink formatting instructions"
+                help="If the tool has no built-in support for your species, i.e., it does not know how to generate hyperlinks for it, you can provide a custom recipe here." />
+            </when>
+            <when value="text">
+                <param name="species" type="hidden" value="" />
+            </when>
+        </conditional>
+    </inputs>
+
+    <outputs>
+        <data name="ofile" format="html">
+            <change_format>
+	            <when input="formatting.oformat" value="text" format="tabular"/>
+            </change_format>
+        </data>
+    </outputs>
+
+    <tests>
+        <test>
+            <param name="inputfile" value="a.vcf" />
+            <conditional name="formatting">
+                <param name="oformat" value="html" />
+                <param name="species" value="C. elegans" />
+            </conditional>
+            <output name="ofile" ftype="html">
+                <extra_files type="file" name="variant_report.html" ftype="html">
+                    <assert_contents>
+                        <has_line_matching expression=".+tools/genome/gbrowse/c_elegans_PRJNA13758.+" />
+                    </assert_contents>
+                </extra_files>
+            </output>
+        </test>
+        <test>
+            <param name="inputfile" value="a.vcf" />
+            <conditional name="formatting">
+                <param name="oformat" value="text" />
+            </conditional>
+            <output name="ofile" ftype="tabular">
+                <assert_contents>
+                    <has_n_columns n="11" />
+                    <has_line_matching expression="Chromosome&#009;Position&#009;Affected Gene&#009;Transcript&#009;Effects&#009;genotype N2&#009;.+genotype ot266&#009;.+" />
+                    <has_line_matching expression="chrI&#009;.+" />
+                </assert_contents>
+            </output>
+        </test>
+    </tests>
+    
+    <help><![CDATA[
+.. class:: infomark
+
+   **What it does**
+
+The tool turns a variant list in VCF format into a more readable summary table
+listing variant sites and effects.
+
+
+**Html output enriched with species-specific hyperlinks**
+
+If you select html as the output format, the tool can insert species- and
+variant-specific hyperlinks to public genome browsers and databases into the
+report. This is a useful feature to explore medium-size lists of variants, but
+requires that the tool knows:
+
+1) the species that you are analyzing data for
+
+   The tool can autodetect the species if the input dataset has been generated
+   with the *MiModD Variant Annotation* tool. Alternatively, you can declare
+   the species you are working with explicitly.
+   
+2) how to generate hyperlinks for this species
+   
+   The tool has built-in support for a number of standard model organisms.
+   If your organism is not in that list or if you find that the default
+   hyperlinks for a supported species are outdated, you can provide your own
+   recipe to generate correct hyperlinks through a `custom hyperlink template
+   file`_.
+      
+   **TIP:**
+   MiModD's built-in hyperlink formatting tables are actively maintained and
+   extended with every new version! If you find the tool produces outdated
+   hyperlinks for any supported species or if you would like to see additional
+   species supported, do not hesitate to `tell us about it`_.
+   If you have a custom hyperlink template file that is working for you, that
+   is even better. We may use it as a starting point for a built-in recipe and,
+   while we are working on that and with your permission, we can post it on the
+   package home page for other users who may need it.
+
+.. _custom hyperlink template file: http://mimodd.readthedocs.io/en/@MIMODD_REAL_VERSION@/recipes.html#hyperlink-template-file
+.. _tell us about it: mailto:mimodd@googlegroups.com
+
+@HELP_FOOTER@
+    ]]></help>
+    <expand macro="citations" />
+</tool>
diff -r 000000000000 -r f0f2795de2c7 vcf_filter.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/vcf_filter.xml	Tue Dec 19 04:54:04 2017 -0500
@@ -0,0 +1,205 @@
+<tool id="mimodd_vcf_filter" name="MiModD VCF Filter" version="@MIMODD_WRAPPER_VERSION@">
+    <description>
+    extracts lines from a vcf variant file based on field-specific filters
+    </description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="stdio" />
+    <expand macro="version_command" />
+    <command><![CDATA[
+	mimodd vcf-filter
+	    '$inputfile' -o '$outputfile'
+	  #if len($datasets):
+	    -s
+	    #for $i in $datasets
+		  '$i.sample'
+	    #end for
+	    --gt
+	    #for $i in $datasets
+	      ## remove whitespace from free-text input
+	      '#echo ("".join($i.GT.split()) or "ANY")#'
+	      #echo " "
+	    #end for
+	    --dp
+	    #for $i in $datasets
+	      $i.DP
+	    #end for
+	    --gq
+	    #for $i in $datasets
+	      $i.GQ
+	    #end for
+	    --af
+	    #for $i in $datasets
+	      '#echo ($i.AF or "::")#'
+	    #end for
+	  #end if
+	  #if len($regions):
+	    -r
+	    #for $i in $regions
+	      #if $i.stop:
+	        '$i.chrom:$i.start-$i.stop'
+	      #else:
+	        '$i.chrom:$i.start'
+	      #end if
+	    #end for
+	  #end if
+	  #if $vfilter:
+	    --vfilter
+	    ## remove ',' and replace with ' '
+	    '#echo ('" "'.join($vfilter.split(',')))#'
+	  #end if
+	  $vartype
+    ]]></command>
+  
+    <inputs>
+        <param name="inputfile" type="data" format="vcf" label="VCF input file" />
+        <repeat name="datasets" title="Sample-specific Filter" default="0" min="0">
+            <param name="sample" type="text" label="sample"
+            help="name of a sample as it appears in the VCF input file and that indicates the sample that this filter should be applied to.">
+                <expand macro="lex_sam_header" message="Non-ASCII characters are not valid in sample names." />
+            </param>
+	        <param name="GT" type="text" 
+	        label="genotype pattern(s) for the inclusion of variants"
+	        help="keep only variants for which the genotype of the sample matches the specified pattern; format: x/x where x = 0 is wildtype and x = 1 is mutant. Multiple genotypes can be specified as a comma-separated list.">
+	            <validator type="expression" message="Malformed genotype pattern">not value or all(c.isdigit() or c in './|' for token in value.split(',') for c in token.strip(' '))</validator>
+	        </param>
+	        <param name="DP" type="integer" value="0" 
+	        label="depth of coverage for the sample at the variant site"
+	        help="keep only variants with at least this sample-specific coverage at the variant site" />
+        	<param name="GQ" type="integer" value="0"
+        	label="genotype quality for the variant in the sample"
+        	help="keep only variants for which the genotype prediction for the sample has at least this quality" />
+	        <param name="AF" type="text"
+	        label="allelic fraction filter"
+	        help="expected format: [allele number]:[minimal fraction]:[maximal fraction]; keep only variants for which the fraction of sample-specific reads supporting a given allele number is between minimal and maximal fraction; if allele number is omitted, the filter operates on the most frequent non-reference allele instead">
+	            <validator type="expression" message="Malformed allelic fraction filter">not value or all(c.isdigit() or c in '.:' for c in value)</validator>
+	        </param>
+        </repeat>
+        <repeat name="regions" title="Region Filter" default="0" min="0"
+        help="Filter variant sites by their position in the genome. If multiple Region Filters are specified, all variants that fall in ONE of the regions are reported.">
+            <param name="chrom" type="text" label="Chromosome">
+                <expand macro="lex_sam_header" message="Non-ASCII characters are not valid in chromosome names." />
+            </param>
+            <param name="start" type="text" label="Region Start">
+                <validator type="expression" message="an integer number is required">not value or value.isdigit()</validator>
+            </param>
+            <param name="stop" type="text" label="Region End">
+                <validator type="expression" message="an integer number is required">not value or value.isdigit()</validator>
+            </param>
+        </repeat>
+        <param name="vartype" type="select" 
+        label="Select the types of variants to include in the output">
+            <option value="">all types of variants</option>
+            <option value="--no-indels">exclude indels</option>
+            <option value="--indels-only">only indels</option>
+        </param>
+        <param name="vfilter" type="text" label="sample"
+        help="Filter output by sample name; only the sample-specific columns with their sample name matching any of the comma separated filters will be retained in the output.">
+            <expand macro="lex_sam_header" message="Non-ASCII characters are not valid in sample names." />
+        </param>
+    </inputs>
+  
+    <outputs>
+        <data name="outputfile" format="vcf" />
+    </outputs>
+
+    <tests>
+        <test>
+            <param name="inputfile" value="a.vcf" />
+            <repeat name="datasets">
+                <param name="sample" value="N2" />
+                <param name="GT" value="0/0" />
+            </repeat>
+            <output name="outputfile" ftype="vcf" compare="diff">
+                <assert_contents>
+                    <has_text text="GT:PL:DP:DPR:GQ&#009;0/0" />
+                    <not_has_text text="GT:PL:DP:DPR:GQ&#009;1/1" />
+                    <not_has_text text="GT:PL:DP:DPR:GQ&#009;0/1" />
+                </assert_contents>
+            </output>
+        </test>
+        <test>
+            <param name="inputfile" value="a.vcf" />
+            <repeat name="regions">
+                <param name="chrom" value="chrX" />
+            </repeat>
+            <output name="outputfile" ftype="vcf">
+                <assert_contents>
+                    <has_text text="chrX&#009;" />
+                    <not_has_text text="chrI&#009;" />
+                    <not_has_text text="chrII&#009;" />
+                    <not_has_text text="chrIII&#009;" />
+                    <not_has_text text="chrIV&#009;" />
+                    <not_has_text text="chrV&#009;" />
+                </assert_contents>
+            </output>
+        </test>
+        <test>
+            <param name="inputfile" value="a.vcf" />
+            <param name="vartype" value="--no-indels" />
+            <param name="vfilter" value="ot266" />
+            <output name="outputfile" ftype="vcf">
+                <assert_contents>
+                    <not_has_text text="INDEL;" />
+                    <has_line line="#CHROM&#009;POS&#009;ID&#009;REF&#009;ALT&#009;QUAL&#009;FILTER&#009;INFO&#009;FORMAT&#009;ot266" />
+                </assert_contents>
+            </output>
+        </test>
+    </tests>
+    
+    <help><![CDATA[
+.. class:: infomark
+
+   **What it does**
+
+The tool filters a variant file in VCF format to generate a new VCF file with only a subset of the original variants.
+
+The following types of variant filters can be set up:
+
+1) Sample-specific filters:
+   
+   Filter variants based on their characteristics in the sequenced reads of a specific sample. Multiple sample-specific filters are combined by logical AND, i.e., only variants that pass ALL sample-specific filters are kept.
+   
+2) Region filters:
+   
+   Filter variants based on the genomic region they affect. Multiple region filters are combined by logical OR, i.e., variants passing ANY region filter are kept.
+   
+3) Variant type filter:
+
+   Filter variants by their type, i.e. whether they are single nucleotide variations (SNVs) or indels
+   
+In addition, the *sample* filter can be used to reduce the samples encoded in a multi-sample VCF file to just those specified by the filter.
+The *sample* filter is included mainly for compatibility reasons: if an external tool cannot deal with the multisample file format, but instead looks only at the first sample-specific column of the file, you can use the filter to turn the multi-sample file into a single-sample file. Besides, the filter can also be used to change the order of the samples since it will sort the samples in the order specified in the filter field.
+
+**Examples of sample-specific filters:**
+
+*Simple genotype pattern*
+
+genotype pattern: 1/1 ==> keep all variants in the vcf input file for which the specified sample's genotype is homozygous mutant
+
+*Complex genotype pattern*
+
+genotype pattern: 0/1, 0/0 ==> keep all variants for which the sample's genotype is either heterozygous or homozygous wildtype
+
+*Multiple sample-specific filters*
+
+Filter 1: genotype pattern: 0/0, Filter 2: genotype pattern 1/1:
+==> keep all variants for which the first sample's gentoype is homozygous wildtype **and** the second sample's genotype is homozygous mutant
+
+*Combining sample-specific filter criteria*
+
+genotype pattern: 1/1, depth of coverage: 3, genotype quality: 9
+==> keep variants for which the sample's genotype is homozygous mutant **and** for which this genotype assignment is corroborated by a genotype quality score of at least 9
+**and** at least three reads from the sample cover the variant site
+
+**TIP:**
+
+As in the example above, genotype quality is typically most useful in combination with a genotype pattern.
+It acts then, effectively, to make the genotype filter more stringent.
+
+@HELP_FOOTER@
+    ]]></help>
+    <expand macro="citations" />
+</tool>