# HG changeset patch # User wolma # Date 1418509206 18000 # Node ID 37e44e7e2d508697741003e8386f7d9f750e0c81 Imported from capsule None diff -r 000000000000 -r 37e44e7e2d50 sam_header.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/sam_header.xml Sat Dec 13 17:20:06 2014 -0500 @@ -0,0 +1,128 @@ + + Create a SAM format header from run metadata for sample annotation. + + mimodd + + mimodd version -q + + mimodd header + + --rg-id "$rg_id" + --rg-sm "$rg_sm" + + #if $str($rg_cn): + --rg-cn "$rg_cn" + #end if + #if $str($rg_ds): + --rg-ds "$rg_ds" + #end if + #if $str($rg_date): + --rg-dt "$rg_date" + #end if + #if $str($rg_lb): + --rg-lb "$rg_lb" + #end if + #if $str($rg_pl): + --rg-pl "$rg_pl" + #end if + #if $str($rg_pi): + --rg-pi "$rg_pi" + #end if + #if $str($rg_pu): + --rg-pu "$rg_pu" + #end if + + --ofile $outputfile + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +.. class:: infomark + + **What it does** + +This tool takes the user-provided information about a next-generation sequencing run and constructs a valid header in the SAM file format from it. + +The result file can be used by the tools *Convert* and *Reheader* or in the *SNAP Read Alignment* step to add run metadata to sequenced reads files (or to overwrite pre-existing information). + +**Note:** + +**MiModD requires run metadata for every input file at the Alignment step !** + +**Tip:** + +While you can do Alignments from fastq file format by providing a custom header file directly to the *SNAP Read Alignment* tool, we **recommend** you to first convert all input files to and archive all datasets in SAM/BAM format with appropriate header information prior to any downstream analysis. Although a bit more time-consuming, this practice protects against information loss and ensures that the input datasets will remain useful for others in the future. + + + + diff -r 000000000000 -r 37e44e7e2d50 tool_dependencies.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Sat Dec 13 17:20:06 2014 -0500 @@ -0,0 +1,6 @@ + + + + + +