diff mytools/align2database.py @ 0:39217fa39ff2

Uploaded

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/mytools/align2database.py	Tue Mar 13 23:34:52 2012 -0400
@@ -0,0 +1,104 @@
+'''
+align mulitple bed to one bed
+python align_multiple.py hmChIP_mm9_peak_bed/mm9-GSE19999_PolII_P25_all.cod.bed hmChIP_mm9_peak_bed/ test.txt test.pdf 100 5000
+'''
+
+import os,sys,random
+def main():
+    queryfile = sys.argv[1]
+    inpath = sys.argv[2]
+    outputdata = sys.argv[3]
+    outputerr = sys.argv[4]
+    barplotpdf = sys.argv[5]
+    min_feat = sys.argv[6] # min features overlap
+    windowsize = sys.argv[7]
+    anchor = sys.argv[8]
+    span = sys.argv[9] # loess smooth parameter
+        
+    inpath = inpath.rstrip('/')
+    #os.system('rm '+inpath+'/*tmp*')
+
+    infiles = os.listdir(inpath)
+
+    #print len(infiles),' files\n'
+    i = 0
+    for infile in infiles:
+        if 'tmp' in infile:
+            #os.system('rm '+inpath+'/'+infile)
+            continue
+        i = i +1
+        print i,infile
+        output = infile.split('/')[-1]+'-to-'+queryfile.split('/')[-1]#'.summary'
+        if anchor == 'database':
+            command = 'python /Users/xuebing/galaxy-dist/tools/mytools/alignr.py -b '+inpath+'/'+infile+' -a '+queryfile+' -o '+output+' --summary-only -q -w '+windowsize
+        else:
+            command = 'python /Users/xuebing/galaxy-dist/tools/mytools/alignr.py -a '+inpath+'/'+infile+' -b '+queryfile+' -o '+output+' --summary-only -q -w '+windowsize            
+        #print command+'\n'
+        os.system(command)
+    print 'start visualization...'
+    # visualize
+    rscriptfile = 'f-'+str(random.random())+'.r'
+    r = open(rscriptfile,'w')
+    r.write("files <- dir('.','summary',full.name=T)\n")
+    #r.write("print(files)\n")    
+    r.write("x <- read.table(files[1])\n")
+    r.write("err <- read.table(gsub('summary','standarderror',files[1]))\n")
+    r.write("for (filename in files[2:length(files)]){\n")
+    r.write("   x <- rbind(x,read.table(filename))\n")
+    r.write("   err <- rbind(err,read.table(gsub('summary','standarderror',filename)))\n")    
+    r.write("}\n")
+    r.write("x <- x[x[,2] > "+min_feat+",]\n")
+    r.write("err <- err[err[,2] > "+min_feat+",]\n")    
+    r.write("name <- as.character(x[,1])\n")
+    r.write("nfeat <- x[,2]\n")
+    r.write("x <- x[,3:ncol(x)]\n")
+    r.write("err <- err[,3:ncol(err)]\n")    
+    r.write("for (i in 1:nrow(x)) {\n")
+    r.write("    name[i] <- strsplit(name[i],'-to-')[[1]][1]\n")
+    r.write("}\n")
+    # remove rows that have no variation, which cause problem in heatmap. This is the case when align to itself
+    r.write("toremove <- seq(nrow(x))\n")
+    r.write("for (i in 1:nrow(x)){\n")
+    r.write("    toremove[i] <- all(x[i,] == x[i,1])\n")
+    r.write("}\n")
+    r.write("x <- x[!toremove,]\n")
+    r.write("err <- err[!toremove,]\n")
+    r.write("name <- name[!toremove]\n")
+    r.write("nfeat <- nfeat[!toremove]\n")
+    r.write("write.table(cbind(name,nfeat,x),file='"+outputdata+"',sep ='\\t',quote=F,row.names=F,col.names=F)\n")
+    r.write("write.table(cbind(name,nfeat,err),file='"+outputerr+"',sep ='\\t',quote=F,row.names=F,col.names=F)\n")
+        
+    r.write("pdf('"+barplotpdf+"')\n")
+    r.write("heatmap(t(scale(t(as.matrix(x,nc=ncol(x))))),Colv=NA,labRow=name,labCol=NA,margins=c(1,8),cexRow=0.02+1/log(nrow(x)),col=heat.colors(1000))\n")
+
+    if windowsize != '0' :
+        r.write("xticks <- seq(-"+windowsize+","+windowsize+",length.out=100)\n")
+        r.write("xlab <- 'relative position (bp)'\n")
+    else:
+        r.write("xticks <- seq(100)\n")
+        r.write("xlab <- 'relative position (bin)'\n")
+        
+    #r.write("plot(xticks,colSums(t(scale(t(as.matrix(x,nc=ncol(x)))))),xlab='relative position (bp)',type='l',lwd=2,main='total signal')\n")
+    r.write("for (i in 1:nrow(x)) {\n")
+    r.write("   avg <- x[i,]/nfeat[i]\n")
+    #r.write("   maxv <- max(avg)\n")
+    #r.write("   minv <- min(avg)\n")
+    #r.write("   medv <- median(avg)\n")
+    #r.write("   if (maxv < "+fold+"*medv | minv*"+fold+">medv){next}\n")
+    #smooth
+    if float(span) >= 0.1:
+        r.write("   avg = loess(as.numeric(avg)~xticks,span="+span+")$fitted\n")
+        r.write("   err[i,] = loess(as.numeric(err[i,])~xticks,span="+span+")$fitted\n")
+    r.write("   par(cex=1.5)\n")
+    r.write("   plot(xticks,avg,ylab='average coverage',main=paste(name[i],'\n n=',nfeat[i],sep=''),xlab=xlab,type='l',lwd=1,ylim=c(min(avg-err[i,]),max(avg+err[i,])))\n")   
+    r.write("   polygon(c(xticks,rev(xticks)),c(avg+err[i,],rev(avg-err[i,])),col='slateblue1',border=NA)\n")
+    r.write("   lines(xticks,avg,type='l',lwd=1)\n")   
+    r.write("}\n")
+    r.write("dev.off()\n")
+    r.close()
+    os.system("R --vanilla < "+rscriptfile)    
+    os.system('rm '+rscriptfile)
+    os.system('rm *.summary')
+    os.system('rm *.standarderror')
+
+main()