annotate tools/picard/picard_BamIndexStats.xml @ 2:c2a356708570

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<tool name="BAM Index Statistics" id="picard_BamIndexStats" version="0.2.0">
  <requirements><requirement type="package">picard</requirement></requirements>
  <command interpreter="python">
    picard_wrapper.py
      --input "$input_file"
      --bai-file "$input_file.metadata.bam_index"
      -t "$htmlfile"
      -d "$htmlfile.files_path"
      -j "${GALAXY_DATA_INDEX_DIR}/shared/jars/BamIndexStats.jar"
  </command>
  <inputs>
    <param format="bam" name="input_file" type="data"  label="BAM dataset to generate statistics for"
      help="If empty, upload or import a BAM dataset" />
  </inputs>
  <outputs>
    <data format="html" name="htmlfile" label="${tool.name}_on_${on_string}.html" />
  </outputs>
  <tests>
    <test>
      <!-- Command
      java -jar BamIndexStats.jar I=test-data/picard_input_tiny_coord.bam > picard_BIS_output1.txt
      picard_input_tiny_coord.bam can be created from picard_input_tiny_coord.sam
      -->
      <param name="input_file" value="picard_input_tiny_coord.bam" ftype="bam" />
      <output name="htmlfile" file="picard_BIS_output1.txt" ftype="html" compare="contains" lines_diff="12"/>
    </test>
    <test>
      <!-- Command
      java -jar BamIndexStats.jar I=test-data/picard_BIS_input1.bam > picard_BIS_output2.txt
      picard_BIS_input1.bam can be created from picard_BIS_input1.sam
      -->
      <param name="input_file" value="picard_BIS_input1.bam" ftype="bam" />
      <output name="htmlfile" file="picard_BIS_output2.txt" ftype="html" compare="contains" lines_diff="12" />
    </test>
  </tests>
  <help>

.. class:: infomark

**Purpose**

Generate Bam Index Stats for a provided BAM file.

**Picard documentation**

This is a Galaxy wrapper for BamIndexStats, a part of the external package Picard-tools_.

 .. _Picard-tools: http://www.google.com/search?q=picard+samtools

------

.. class:: infomark

**Inputs and outputs**

The only input is the BAM file you wish to obtain statistics for, which is required.
Note that it must be coordinate-sorted. Galaxy currently coordinate-sorts all BAM files.

This tool outputs an HTML file that contains links to the actual metrics results, as well
as a log file with info on the exact command run.

.. class:: warningmark

**Warning on SAM/BAM quality**

Many SAM/BAM files produced externally and uploaded to Galaxy do not fully conform to SAM/BAM specifications. Galaxy deals with this by using the **LENIENT**
flag when it runs Picard, which allows reads to be discarded if they're empty or don't map. This appears
to be the only way to deal with SAM/BAM that cannot be parsed.

------

**Example**

Given a BAM file created from the following::

  @HD    VN:1.0     SO:coordinate
  @SQ    SN:chr1    LN:101
  @SQ    SN:chr7    LN:404
  @SQ    SN:chr8    LN:202
  @SQ    SN:chr10   LN:303
  @SQ    SN:chr14   LN:505
  @RG    ID:0       SM:Hi,Mom!
  @RG    ID:1       SM:samplesample    DS:ClearDescription
  @PG    ID:1       PN:Hey!   VN:2.0
  @CO    Just a generic comment to make the header longer
  read1     83    chr7      1    255    101M             =       302     201    CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN    )'.*.+2,))II'I*/)-I*-)I.-)I)I),/-II..)./.,.).*II,I.II-)III0*IIIIIIII/32/,01460II/6/*0*/2/283//36868/I    RG:Z:0
  read2     89    chr7      1    255    101M             *         0       0    CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN    )'.*.+2,))II'I*/)-I*-)I.-)I)I),/-II..)./.,.).*II,I.II-)III0*IIIIIIII/32/,01460II/6/*0*/2/283//36868/I    RG:Z:0
  read3     83    chr7      1    255    101M             =       302     201    CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN    )'.*.+2,))II'I*/)-I*-)I.-)I)I),/-II..)./.,.).*II,I.II-)III0*IIIIIIII/32/,01460II/6/*0*/2/283//36868/I    RG:Z:0
  read4    147    chr7     16    255    101M             =        21     -96    CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN    )'.*.+2,))II'I*/)-I*-)I.-)I)I),/-II..)./.,.).*II,I.II-)III0*IIIIIIII/32/,01460II/6/*0*/2/283//36868/I    RG:Z:0
  read5     99    chr7     21    255    101M             =        16      96    CAACAGAAGCNGGNATCTGTGTTTGTGTTTCGGATTTCCTGCTGAANNGNTTNTCGNNTCNNNNNNNNATCCCGATTTCNTTCCGCAGCTNACCTCCCAAN    )'.*.+2,))II'I*/)-I*-)I.-)I)I),/-II..)./.,.).*II,I.II-)III0*IIIIIIII/32/,01460II/6/*0*/2/283//36868/I    RG:Z:0
  read6    163    chr7    302    255    101M             =         1    -201    NCGCGGCATCNCGATTTCTTTCCGCAGCTAACCTCCCGACAGATCGGCAGCGCGTCGTGTAGGTTATTATGGTACATCTTGTCGTGCGGCNAGAGCATACA    I/15445666651/566666553+2/14/I/555512+3/)-'/-I-'*+))*''13+3)'//++''/'))/3+I*5++)I'2+I+/*I-II*)I-./1'1    RG:Z:0
  read7    163    chr7    302    255    10M1D10M5I76M    =         1    -201    NCGCGGCATCNCGATTTCTTTCCGCAGCTAACCTCCCGACAGATCGGCAGCGCGTCGTGTAGGTTATTATGGTACATCTTGTCGTGCGGCNAGAGCATACA    I/15445666651/566666553+2/14/I/555512+3/)-'/-I-'*+))*''13+3)'//++''/'))/3+I*5++)I'2+I+/*I-II*)I-./1'1    RG:Z:0
  read8    165       *      0      0    *                chr7      1       0    NCGCGGCATCNCGATTTCTTTCCGCAGCTAACCTCCCGACAGATCGGCAGCGCGTCGTGTAGGTTATTATGGTACATCTTGTCGTGCGGCNAGAGCATACA    I/15445666651/566666553+2/14/I/555512+3/)-'/-I-'*+))*''13+3)'//++''/'))/3+I*5++)I'2+I+/*I-II*)I-./1'1    RG:Z:0

The following metrics file will be produced::

  chr1 length=    101    Aligned= 0    Unaligned= 0
  chr7 length=    404    Aligned= 7    Unaligned= 0
  chr8 length=    202    Aligned= 0    Unaligned= 0
  chr10 length=   303    Aligned= 0    Unaligned= 0
  chr14 length=   505    Aligned= 0    Unaligned= 0
  NoCoordinateCount= 1

  </help>
</tool>