comparison tools/peak_calling/ccat_wrapper.xml @ 0:9071e359b9a3

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0:9071e359b9a3

<tool id="peakcalling_ccat" name="CCAT" version="0.0.1">
  <description>Control-based ChIP-seq Analysis Tool</description>
  <command interpreter="python">ccat_wrapper.py '$input_tag_file' '$input_control_file' '$chromInfo' 
  #if str( $options_type[ 'options_type_selector' ] ) == 'advanced':
  '$input_advanced_config_file' 
  #else:
  '${ options_type.input_config_file.fields.path }'
  #end if
  'CCAT in Galaxy' 
  '$output_peak_file' '$output_region_file' '$output_top_file' '$output_log_file'</command>
  <requirements>
    <requirement type="binary" version="3.0">CCAT</requirement>
  </requirements>
  <inputs>
    <param name="input_tag_file" type="data" format="bed" label="ChIP-Seq Tag File" >
      <validator type="unspecified_build" />
    </param>
    <param name="input_control_file" type="data" format="bed" label="ChIP-Seq Control File" >
      <validator type="unspecified_build" />
    </param>
    <conditional name="options_type">
      <param name="options_type_selector" type="select" label="Advanced Options">
        <option value="basic" selected="True">Hide Advanced Options</option>
        <option value="advanced">Show Advanced Options</option>
      </param>
      <when value="basic">
        <param name="input_config_file" type="select" label="Select a pre-defined configuration file">
          <options from_data_table="ccat_configurations">
            <validator type="no_options" message="No configurations are available"/>
          </options>
        </param>
      </when>
      <when value="advanced">
        <param name="fragment_size" type="integer" label="Length of DNA fragment" value="200"/>
        <param name="sliding_window_size" type="integer" label="Sliding window size" value="500" help="transcription factor binding default: 300; histone modifications default: 500"/>
        <param name="moving_step" type="integer" label="Step of sliding window" value="50" help="transcription factor binding default: 10; histone modifications default: 50"/>
        <param name="is_strand_sensitive_mode" type="select" label="isStrandSensitiveMode" >
          <option value="1">Transition from sense strand to anti-sense strand</option>
          <option value="0" selected="True">Local maximum of read-enrichment profile</option>
        </param>
        <param name="min_count" type="integer" label="Minimum number of read counts at the peak" value="4"/>
        <param name="output_num" type="integer" label="Number of peaks reported in top peak file" value="100000"/>
        <param name="random_seed" type="integer" label="Random Seed" value="123456"/>
        <param name="min_score" type="float" label="Minimum score of normalized difference" value="3.0"/>
        <param name="bootstrap_pass" type="integer" label="Number of passes in the bootstrapping process" value="50"/>
      </when>
    </conditional>
  </inputs>
  <outputs>
    <data name="output_peak_file" format="interval" label="${tool.name} on ${on_string} (peaks)">
      <actions>
        <action type="metadata" name="chromCol" default="1"/>
        <action type="metadata" name="startCol" default="3"/>
        <action type="metadata" name="endCol" default="4"/>
      </actions>
    </data>
    <data name="output_region_file" format="interval" label="${tool.name} on ${on_string} (regions)">
      <actions>
        <action type="metadata" name="chromCol" default="1"/>
        <action type="metadata" name="startCol" default="3"/>
        <action type="metadata" name="endCol" default="4"/>
      </actions>
    </data>
    <data name="output_top_file" format="interval" label="${tool.name} on ${on_string} (top peaks)">
      <actions>
        <action type="metadata" name="chromCol" default="1"/>
        <action type="metadata" name="startCol" default="3"/>
        <action type="metadata" name="endCol" default="4"/>
      </actions>
    </data>
    <data name="output_log_file" format="txt" label="${tool.name} on ${on_string} (log)"/>
  </outputs>
  <configfiles>
    <configfile name="input_advanced_config_file">#if str( $options_type['options_type_selector' ] ) == 'advanced':
fragmentSize	${options_type[ 'fragment_size' ]}
slidingWinSize	${options_type[ 'sliding_window_size' ]}
movingStep	${options_type[ 'moving_step' ]}
isStrandSensitiveMode	${options_type[ 'is_strand_sensitive_mode' ]}
minCount	${options_type[ 'min_count' ]}
outputNum	${options_type[ 'output_num' ]}
randomSeed	${options_type[ 'random_seed' ]}
minScore	${options_type[ 'min_score' ]}	
bootstrapPass	${options_type[ 'bootstrap_pass' ]}
#end if</configfile>
  </configfiles>
  <tests>
    <test>
      <param name="input_tag_file" value="chipseq_enriched.bed.gz" ftype="bed" dbkey="hg18" />
      <param name="input_control_file" value="chipseq_input.bed.gz" ftype="bed" dbkey="hg18" />
      <param name="options_type_selector" value="advanced" />
      <param name="fragment_size" value="200" />
      <param name="sliding_window_size" value="500" />
      <param name="moving_step" value="50" />
      <param name="is_strand_sensitive_mode" value="0" />
      <param name="min_count" value="4" />
      <param name="output_num" value="100000" />
      <param name="random_seed" value="123456" />
      <param name="min_score" value="5.0" />
      <param name="bootstrap_pass" value="50" />
      <output name="output_peak_file" file="peakcalling_ccat/3.0/ccat_test_peak_out_1.interval.re_match" compare="re_match" />
      <output name="output_region_file" file="peakcalling_ccat/3.0/ccat_test_region_out_1.interval.re_match" compare="re_match" />
      <output name="output_top_file" file="peakcalling_ccat/3.0/ccat_test_top_out_1.interval.sorted.re_match" compare="re_match" sort="True" />
      <output name="output_log_file" file="peakcalling_ccat/3.0/ccat_test_log_out_1.txt" />
    </test>
    <test>
      <param name="input_tag_file" value="chipseq_enriched.bed.gz" ftype="bed" dbkey="hg18" />
      <param name="input_control_file" value="chipseq_input.bed.gz" ftype="bed" dbkey="hg18" />
      <param name="options_type_selector" value="basic" />
      <param name="input_config_file" value="ccat_3.0_histone_config" />
      <output name="output_peak_file" file="peakcalling_ccat/3.0/ccat_test_peak_out_1.interval.re_match" compare="re_match" />
      <output name="output_region_file" file="peakcalling_ccat/3.0/ccat_test_region_out_1.interval.re_match" compare="re_match" />
      <output name="output_top_file" file="peakcalling_ccat/3.0/ccat_test_top_out_1.interval.sorted.re_match" compare="re_match" sort="true" />
      <output name="output_log_file" file="peakcalling_ccat/3.0/ccat_test_log_out_1.txt" />
    </test>
    <!-- Test below gives different results on different architectures, 
    e.g.: x86_64 GNU/Linux gave an extra line (additional peak called) when compared to the version running on 10.6.0 Darwin i386 
    slidingWinSize was fixed to be 1000, default as per readme.txt
    -->
    <!--
    <test>
      <param name="input_tag_file" value="chipseq_enriched.bed.gz" ftype="bed" dbkey="hg18" />
      <param name="input_control_file" value="chipseq_input.bed.gz" ftype="bed" dbkey="hg18" />
      <param name="options_type_selector" value="basic" />
      <param name="input_config_file" value="ccat_3.0_histone_config_readme" />
      <output name="output_peak_file" file="peakcalling_ccat/3.0/ccat_test_peak_out_2.interval.re_match" compare="re_match" />
      <output name="output_region_file" file="peakcalling_ccat/3.0/ccat_test_region_out_2.interval.re_match" compare="re_match" />
      <output name="output_top_file" file="peakcalling_ccat/3.0/ccat_test_top_out_2.interval.sorted.re_match" compare="re_match" sort="true" />
      <output name="output_log_file" file="peakcalling_ccat/3.0/ccat_test_log_out_2.txt" />
    </test>
  -->
  </tests>
  <help>
**What it does**

This tool allows ChIP-seq peak/region calling using CCAT.

View the original CCAT documentation: http://cmb.gis.a-star.edu.sg/ChIPSeq/paperCCAT.htm.
  </help>
</tool>